Manual sample applicator for antibiotic titer determination

Abstract

The utility model discloses a manual sample applicator for antibiotic titer determination, which comprises a shell, a storage battery mounted in the shell, a charging interface mounted on one side of the storage battery, a plurality of grooves movably connected with steel cups above a disc, a dropping mechanism arranged above the shell, a feeding mechanism arranged above the dropping mechanism, and a discharging mechanism arranged above the feeding mechanism. The outer side of the vertical cylinder is fixedly connected with the side face of the shell through a connecting rod, the lower portion of the outer side of the vertical cylinder is communicated with a plurality of inclined pipes, electric ball valves are installed at the bottoms of the inclined pipes, the bottoms of the electric ball valves are communicated with dropwise adding pipes, and the outer sides of the dropwise adding pipes are fixedly connected with a top plate. The utility model relates to the technical field of antibiotic sample application, realizes synchronous and equivalent addition of a plurality of steel cups through the matching of an electric ball valve, a dropping pipe and a top plate, and solves the problem that the existing sample application device cannot realize synchronous addition and is easy to diffuse in a sequential dropping process.

Description

Technical Field

[0001] The utility model relates to the technical field of sample spotting for determination of antibiotic potency, in particular to a manual sample spotter for determination of antibiotic potency. Background Art

[0002] Antibiotic microbiological assay, also known as antibiotic potency determination method, is a method that uses the characteristics of antibiotics that can selectively inhibit or kill microorganisms at low concentrations, and uses the antibacterial activity of antibiotics as an indicator to measure the effectiveness of the active ingredients in antibiotics. Among them, the tube-plate assay method for antibiotic microbiological assay is the agar diffusion method, which is to place a stainless steel tube (Oxford cup) on the agar medium plate on which the test bacteria are spread, and add antibiotic solution to the tube. Under the culture conditions, the test bacteria begin to grow, and the antibiotic solution diffuses spherically in the agar medium. When a certain period of time has passed, the diffusion reaches a dynamic equilibrium, and a transparent antibacterial zone is formed on the agar medium. The sizes of the inhibition zones produced by the standard and the test sample under the same experimental conditions can be compared, and then the potency of the test sample can be calculated from the known potency of the standard. For example, an antibiotic quantitative detection device with application number "CN202321353209.7" relates to the technical field, including a workbench, on which there is an SPR detector, a piston rod in the injection mechanism will compress the antibiotic sample and it will enter the SPR detector along the third V-shaped tube, the SPR detector will detect and analyze the antibiotic sample, and the SPR detector will display the detection and analysis data on a display screen.

[0003] In the actual antibiotic potency determination process, the test results are still affected by certain factors, causing the test results to deviate from the true value. This phenomenon is related to the characteristics of the tube-plate method, which has many steps, a long experimental period, and a high degree of manual dependence, which is difficult for novices to master. Among them, the standardization and proficiency of the spotting order and spotting time will have a serious impact on the potency determination results of the tube-plate method, and need to be strictly controlled from all aspects of the experiment.

[0004] However, manual spotting cannot achieve synchronous addition of solutions, and thus diffusion asynchrony is prone to occur during the process of sequentially adding solutions, resulting in deviations in the results of antibiotic potency determination. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides a manual spotter for antibiotic potency determination, which solves the problem that manual spotting cannot achieve synchronous addition of solution, and thus diffusion asynchrony is prone to occur during the process of personnel operating the sequential dropwise addition of solution, resulting in deviations in the antibiotic potency determination results.

[0006] To achieve the above-mentioned purpose, the utility model is implemented through the following technical solutions: a manual spotter for determining the potency of antibiotics, comprising a shell, a battery installed inside the shell, a control button and a console installed on the top of the shell, a disc movably connected to the groove on the top of the shell, a groove processed above the disc, a flat plate movably connected to the inner side of the groove, a plurality of steel cups placed inside the flat plate, a dripping mechanism is arranged above the shell, the dripping mechanism comprises a connecting rod, a vertical cylinder, an inclined tube, a partition, an electric ball valve, a dripping tube and a top plate, the outer side of the vertical cylinder is fixedly connected to the side of the shell through a connecting rod, a plurality of inclined tubes are connected below the outer side of the vertical cylinder, electric ball valves are installed at the bottom of the plurality of inclined tubes, the bottom of the electric ball valve is connected to the dripping tube, the outer side of the dripping tube is fixedly connected to the top plate, and a plurality of partitions are installed inside the vertical cylinder.

[0007] Preferably, a charging port is installed on one side of the battery.

[0008] Preferably, the outer side of the disc is fixedly connected to an arc-shaped plate fixed to the top of the housing, and the inner side of the arc-shaped plate is tightly pressed against the outer side of the disc by bolts.

[0009] Preferably, a liquid inlet pipe corresponding to the bottom of the partition is installed above the side of the vertical cylinder.

[0010] Preferably, screws are threadedly connected to both sides of the circular plate, two threaded holes are processed on the top of the vertical cylinder, the screws are threadedly connected to the threaded holes, and a plurality of partitions are fixedly connected to the bottom of the circular plate with straight rods fixed to the inner side.

[0011] Beneficial Effects

[0012] The utility model provides a manual spotter for measuring the potency of antibiotics. The utility model has the following beneficial effects: the manual spotter for measuring the potency of antibiotics achieves synchronous equal spotting of multiple steel cups through the cooperation between the electric ball valve, the dripping tube and the top plate, solves the problem that manual sample addition cannot achieve synchronous addition of solution, and then the diffusion is easily asynchronous during the process of sequential dripping of solution by personnel, resulting in deviation in the result of antibiotic potency measurement.

[0013] Through the cooperation between the straight rod, the partition and the circular plate, the removal and disassembly of multiple partitions are realized, which is convenient for users to clean. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the structure of the utility model;

[0015] Figure 2 for Figure 1 Schematic diagram of the structure of the middle circular plate, screws and partitions;

[0016] Figure 3 for Figure 1 Schematic diagram of the structure of the middle shell, disc and curved plate;

[0017] Figure 4 for Figure 1 Schematic diagram of the structure of the middle vertical cylinder, liquid inlet pipe and threaded hole.

[0018] In the figure: 1. outer shell; 2. battery; 3. charging port; 4. disc; 5. steel cup; 6. connecting rod; 7. vertical cylinder; 8. inclined tube; 9. electric ball valve; 10. drip tube; 11. top plate; 12. liquid inlet pipe; 13. threaded hole; 14. round plate; 15. screw; 16. partition; 17. control console; 18. control button; 19. bolt; 20. arc plate; 21. trough; 22. straight rod; 23. flat dish. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0020] Manual spotting cannot achieve synchronous addition of solutions, and diffusion asynchrony is likely to occur during the process of adding solutions in sequence, resulting in deviations in the results of antibiotic potency determination.

[0021] In view of this, a manual spotter for antibiotic potency determination is provided. Through the cooperation between the electric ball valve, the dripping tube and the top plate, synchronous and equal spotting of multiple steel cups is realized, which solves the problem that manual sampling cannot achieve synchronous addition of solution, and diffusion asynchrony is likely to occur during the process of personnel operating the dripping of solution in sequence, resulting in deviations in the antibiotic potency determination results.

[0022] Through the personnel in this field, all the electrical components in this case are connected to their corresponding power supplies through wires, and appropriate controllers and encoders should be selected according to actual conditions to meet the control requirements. The specific connection and control sequence should refer to the following working principle, and the electrical connection between the electrical components is completed in the order of working in sequence. The detailed connection means are well-known technologies in this field. The following mainly introduces the working principles and processes, and no longer explains the electrical control.

[0023] Embodiment 1: By Figure 1 , 3As shown in Figures 4 and 5, a manual spotter for determining the potency of antibiotics comprises a housing 1, a battery 2 is installed inside the housing 1, a charging interface 3 can supply power to the battery 2, a control button 18 and a console 17 are installed on the top of the housing 1, the control button 18 realizes the human-computer interaction between the console 17 and a person, and can be used to control the dripping of the electric ball valve 9, the battery 2 can supply power to the console 17, a disc 4 is movably connected to the groove on the top of the housing 1, a groove 21 is machined above the disc 4, a plate 23 is movably connected to the inner side of the groove 21, the groove 21 matches the shape of the plate 23, a plurality of steel cups 5 are placed inside the plate 23, a dripping mechanism is arranged on the top of the housing 1, the dripping mechanism comprises a connecting rod 6, a vertical cylinder 7, an inclined tube 8, a partition 16, an electric ball valve 9 , a dripping tube 10 and a top plate 11. The outer side of the vertical cylinder 7 is fixedly connected to the side of the shell 1 through a connecting rod 6. A plurality of inclined tubes 8 are connected to the lower part of the outer side of the vertical cylinder 7. The plurality of inclined tubes 8 are connected to different heights on the outer side of the vertical cylinder 7, and the connecting port position is arranged above the position of the partition 16 to avoid the accumulation of liquid between adjacent partitions 16. The samples stored between adjacent partitions 16 and the vertical cylinder 7 are different, including control and experimental samples, etc. Electric ball valves 9 are installed at the bottom of the plurality of inclined tubes 8. The control console 17 includes a controller, etc., which can synchronously control the start and stop of the plurality of electric ball valves 9, thereby realizing synchronous unloading. The model of the electric ball valve 9 is not limited. The bottom of the electric ball valve 9 is connected to a dripping tube 10. The outer side of the dripping tube 10 is fixedly connected to the top plate 11. The interior of the vertical cylinder 7 is movably connected to a plurality of partitions 16.

[0024] In the specific implementation process, it is worth pointing out that the electric ball valve 9, the dripping tube 10 and the top plate 11 are used to achieve synchronous equal amount of sample addition to multiple steel cups 5, which solves the problem that manual sample addition cannot achieve synchronous addition of solution, and then the diffusion is prone to asynchronous in the process of dripping the solution in sequence by the operator, resulting in deviation in the result of antibiotic potency determination;

[0025] Furthermore, a charging interface 3 is installed on one side of the battery 2;

[0026] In the specific implementation process, it is worth pointing out that the charging interface 3 can be connected to an external power supply wire for charging;

[0027] Furthermore, the outer side of the disc 4 is fixedly connected to an arc plate 20 fixed to the top of the housing 1, and the inner side of the arc plate 20 is tightly pressed against the outer side of the disc 4 by bolts 19;

[0028] In the specific implementation process, it is worth pointing out that the user can rotate the bolt 19 to tighten against the outer side of the disc 4 to fix it, so as to prevent the disc 4 from moving randomly;

[0029] Specifically, when using the manual spotter for antibiotic potency determination, the user can place the steel cup 5 in the corresponding groove on the disc 4, and then send a signal to the console 17 through the control button 18. The console 17 can control multiple electric ball valves 9 to start dripping into the steel cup 5 synchronously. For example, the liquid output can be controlled between 300 microliters and 320 microliters.

[0030] Embodiment 2: By Figure 1-4 It can be seen that the liquid inlet pipe 12 corresponding to the bottom of the partition 16 is installed on the upper side of the vertical cylinder 7. Each liquid inlet pipe 12 is respectively added with different types of liquids. The liquid inlet pipe 12 is arranged below the partition 16 to avoid liquid backflow and make the liquid inlet pipe 12 at the highest point.

[0031] In the specific implementation process, it is worth pointing out that the liquid inlet pipe 12 can realize the filling of liquid;

[0032] Furthermore, screws 15 are threadedly connected to both sides of the circular plate 14, two threaded holes 13 are processed on the top of the vertical tube 7, the screws 15 are threadedly connected to the threaded holes 13, a liquid inlet pipe 12 is installed on the upper side of the vertical tube 7, and a plurality of straight rods 22 fixed to the inner side of the partition 16 are fixedly connected to the bottom of the circular plate 14;

[0033] In the specific implementation process, it is worth pointing out that the circular plate 14 can be fixed on the threaded hole 13 of the vertical cylinder 7 by means of screws 15;

[0034] Specifically, on the basis of the above-mentioned first embodiment, through the cooperation between the straight rod 22, the partition 16 and the circular plate 14, the removal and disassembly of the plurality of partitions 16 are realized, which is convenient for the user to clean.

[0035] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions. The sentence "including an element defined by... does not exclude the existence of other identical elements in the process, method, article or device including the element".

[0036] In the present utility model, unless otherwise clearly stipulated and limited, the terms such as "installation", "setting", "connection", "fixation" and "screw-on" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. Unless otherwise clearly defined, ordinary technicians in this field can understand the specific meanings of the above terms in the utility model according to the specific circumstances.

[0037] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A manual spotter for determining the potency of antibiotics, comprising a housing (1), characterized in that: A storage battery (2) is installed inside the housing (1); a control button (18) and a control console (17) are installed on the top of the housing (1); a disc (4) is movably connected to a groove on the top of the housing (1); a groove (21) is machined on the top of the disc (4); a plate (23) is movably connected to the inside of the groove (21); a plurality of steel cups (5) are placed inside the plate (23); and a dripping mechanism is arranged on the top of the housing (1); The dripping mechanism comprises a connecting rod (6), a vertical cylinder (7), an inclined tube (8), a partition (16), an electric ball valve (9), a dripping tube (10) and a top plate (11); The outer side of the vertical cylinder (7) is fixedly connected to the side of the outer shell (1) through a connecting rod (6); a plurality of inclined tubes (8) are connected below the outer side of the vertical cylinder (7); an electric ball valve (9) is installed at the bottom of each of the plurality of inclined tubes (8); a dripping tube (10) is connected at the bottom of the electric ball valve (9); a top plate (11) is fixedly connected to the outer side of the dripping tube (10); and a plurality of partitions (16) are installed inside the vertical cylinder (7).

2. A manual spotter for determining the potency of antibiotics according to claim 1, characterized in that: A charging interface (3) is installed on one side of the storage battery (2).

3. A manual spotter for determining the potency of antibiotics according to claim 2, characterized in that: The outer side of the disc (4) is fixedly connected to an arc-shaped plate (20) fixed to the top of the housing (1), and the inner side of the arc-shaped plate (20) is tightly pressed against the outer side of the disc (4) via bolts (19).

4. A manual spotter for determining the potency of antibiotics according to claim 1, characterized in that: A liquid inlet pipe (12) corresponding to the bottom of the partition plate (16) is installed above the side of the vertical cylinder (7).

5. A manual spotter for determining the potency of antibiotics according to claim 1, characterized in that: Screws (15) are threadedly connected to both sides of the circular plate (14); two threaded holes (13) are processed on the top of the vertical cylinder (7); the screws (15) are threadedly connected to the threaded holes (13); and a plurality of partitions (16) and straight rods (22) fixed to the inner side are fixedly connected to the bottom of the circular plate (14).

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