Liquid supplementing device

Abstract

The utility model discloses a liquid supplementing device which comprises a culture box, an electric push rod is fixedly connected to the top of the culture box, a liquid supplementing mechanism is arranged in the culture box, two silica gel gloves are fixedly embedded in the outer wall of the culture box, a machine box is fixedly connected to the interior of the culture box, and a motor is fixedly connected to the interior of the machine box. By arranging the electric push rod, the transparent liquid supplementing box, the piston, the transparent acrylic plate, the telescopic liquid supplementing pipe and the silica gel gloves, operation can be carried out without opening a sealing door, interference of external factors is reduced, accuracy is improved, and by arranging the machine box, the motor, the disc, the partition plate, the fan-shaped plate, the circular rubber column, the silica gel anti-skid pad and the arc-shaped plate, operation is convenient, and operation is convenient. The rotatable fan-shaped plates can be lifted for culture bags or transverse culture bottles to assist in liquid supplementing operation, liquid leakage is avoided, meanwhile, the disc rotates to drive the multiple fan-shaped plates to be switched, liquid supplementing can be conveniently conducted on the multiple culture devices, and the overall applicability is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a fluid replenishing device. Background Art

[0002] Cell culture refers to the process of separating cells from the body or outside the body and allowing them to continue to grow and reproduce in vitro through specific culture media and conditions. Cell culture is one of the most important experimental techniques in biology, medicine, and biotechnology. During the cell culture process in the culture vessel, the cells will consume the culture fluid in the incubator, so the staff needs to replenish the incubator regularly.

[0003] After searching, the patent document with publication number: CN217809495U discloses a fluid replenishing device for cell culture; it is provided with a sealing cover, a switch, a battery, a driving motor, a screw, a threaded sleeve, a rubber plate, a liquid outlet pipe, a flow detector, an infusion tube, a liquid inlet pipe, and a valve. When the culture medium is replenished to the incubator, the left end of the infusion tube is sleeved on the lower end of the liquid outlet pipe, and the right end is sleeved on the left end of the liquid inlet pipe. During the replenishing process, the incubator does not need to be opened to avoid affecting the growth of cells inside the incubator. The switch is pressed to energize the driving motor, and the driving motor drives the screw to rotate. The screw pushes the rubber plate at the lower end of the threaded sleeve downward, and at the same time the valve is opened, and the rubber plate moves downward to transport the culture medium inside the liquid storage cylinder through the infusion tube into the incubator. During the culture medium transportation process, the flow detector monitors the culture medium transportation capacity in real time, and the staff can press the switch to stop replenishing the culture medium at any time.

[0004] Although the above-mentioned device has solved some of the existing shortcomings, in actual use, since the types of culture vessels include culture bags, culture dishes and culture bottles, etc., cells need to be placed in an incubator for culture during the culture process to maintain the required temperature, humidity, oxygen and carbon dioxide conditions. After the above-mentioned device transports the culture solution to the incubator, it can only culture a single culture vessel in the incubator. When a control test is required, another identical device is also required, which has low applicability. Utility Model Content

[0005] The utility model aims to solve the shortcomings in the prior art and proposes a fluid replenishing device.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A fluid replenishment device comprises an incubator, wherein an electric push rod is fixedly connected to the top of the incubator, a fluid replenishment mechanism is arranged inside the incubator, two silicone gloves are fixedly inlaid on the outer wall of the incubator, the interior of the incubator is fixedly connected to a machine box, the interior of the machine box is fixedly connected to a motor, a disc is fixedly connected to the top of the motor output shaft, a plurality of partitions are fixedly connected to the top of the disc, a plurality of fan-shaped plates are hinged on the outer wall of the disc, and an anti-slip mechanism is arranged on the outer wall of the fan-shaped plates.

[0008] Preferably, the fluid replenishment mechanism includes a transparent fluid replenishment box, the inner wall of the incubator is fixedly connected to the top of the transparent fluid replenishment box, the inner wall of the transparent fluid replenishment box is slidably connected with a piston, the bottom of the output end of the electric push rod is fixedly connected to the top of the piston, and a telescopic fluid replenishment tube is fixedly connected to the bottom of the transparent fluid replenishment box. By setting up the telescopic fluid replenishment tube, the tube can be retracted and extended, mainly by utilizing its special structural design and material properties, so that it can retract and deform itself under the action of external forces.

[0009] Preferably, the anti-slip mechanism includes a plurality of arc-shaped plates, the outer wall of the fan-shaped plate is fixedly connected to two circular rubber columns, the circular rubber columns are squeezed against the partition, the outer wall of the fan-shaped plate is fixedly inlaid with a silicone anti-slip pad, the bottoms of the plurality of arc-shaped plates are respectively fixedly connected to the tops of the plurality of fan-shaped plates, and the culture bottles and culture bags are supported by arranging the arc-shaped plates and the silicone anti-slip pads to prevent them from slipping out of the fan-shaped plates when tilted.

[0010] Preferably, the tops of the incubator and the transparent liquid replenishment tank are both provided with through holes, the inner wall of the through hole is slidably connected to the outer wall of the output end of the electric push rod, and the electric push rod is provided to drive the piston to move up and down.

[0011] Preferably, the outer wall of the culture box is fixedly inlaid with a transparent acrylic plate, and the injection amount of the cell culture fluid in the transparent fluid replenishment box can be observed by setting the transparent acrylic plate, and the outer wall of the transparent fluid replenishment box is provided with scale lines.

[0012] Preferably, a circular hole is opened on the top of the chassis, the inner wall of the circular hole is rotatably connected to the outer wall of the motor output shaft, and the motor is provided to drive the disc to rotate.

[0013] Preferably, a ball valve is fixedly mounted on the outer wall of the telescopic infusion tube, and a sealing door is hingedly mounted on the outer wall of the incubator, and the outflow of the cell culture fluid is controlled by setting the ball valve.

[0014] Compared with the prior art, the advantages of the utility model are:

[0015] This solution uses an electric push rod, a transparent fluid replenishment tank, a piston, a transparent acrylic plate, a telescopic fluid replenishment tube, and silicone gloves, and does not require the sealed door to be opened for operation, thereby reducing interference from external factors and improving accuracy.

[0016] By arranging a chassis, a motor, a disc, a partition, a fan-shaped plate, a circular rubber column, a silicone anti-skid pad and an arc-shaped plate, the fan-shaped plate can be rotated to lift the culture bag or the horizontal culture bottle to assist the liquid replenishment operation and avoid leakage. At the same time, the rotation of the disc drives the switching of multiple fan-shaped plates, which is convenient for liquid replenishment of multiple culture vessels, thereby increasing the overall applicability. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solution of the present invention, the drawings required for use in the description of the specific implementation methods will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 This is a three-dimensional structural schematic diagram of a fluid replenishing device proposed by the utility model;

[0019] Figure 2 This is a schematic cross-sectional structure diagram of a fluid replenishing device proposed by the utility model;

[0020] Figure 3 A liquid replenishing device proposed by the utility model Figure 2 A schematic diagram of the enlarged structure of part A;

[0021] Figure 4 The utility model provides a schematic structural diagram of a disc, a partition, a fan-shaped plate, a circular rubber column and a silicone anti-skid pad of a liquid replenishing device.

[0022] In the figure: 1. incubator; 2. electric push rod; 3. transparent rehydration tank; 4. piston; 5. transparent acrylic plate; 6. telescopic rehydration tube; 7. silicone gloves; 8. chassis; 9. motor; 10. disc; 11. partition; 12. fan-shaped plate; 13. round rubber column; 14. silicone anti-slip pad; 15. curved plate. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Depend on Figure 1-Figure 4As shown, a fluid replenishment device is involved, including an incubator 1, the top of the incubator 1 is fixedly connected to an electric push rod 2, and two existing foot switches are installed on the outer wall of the incubator 1. The foot switch is based on the electromagnetic theorem and the contact control principle. When stepping on the pedal, the pedal will push the contact to close or disconnect. The closed contact will be energized to make the electrical appliance work, and the disconnected contact will stop energizing and the electrical appliance stops working. The tops of the incubator 1 and the transparent fluid replenishment tank 3 are both provided with through holes, and the inner wall of the through hole is slidably connected to the outer wall of the output end of the electric push rod 2.

[0025] Two silicone gloves 7 are fixedly inlaid on the outer wall of the incubator 1, the interior of the incubator 1 is fixedly connected to a chassis 8, the interior of the chassis 8 is fixedly connected to a motor 9, a circular hole is opened on the top of the chassis 8, the inner wall of the circular hole is rotatably connected to the outer wall of the output shaft of the motor 9, a disc 10 is fixedly connected to the top of the output shaft of the motor 9, a plurality of partitions 11 are fixedly connected to the top of the disc 10, a plurality of fan-shaped plates 12 are hinged on the outer wall of the disc 10, the operation of the motor 9 drives the disc 10 to rotate, and the rotation of the disc 10 drives the plurality of fan-shaped plates 12 to rotate.

[0026] A fluid replenishment mechanism is provided inside the incubator 1, and the fluid replenishment mechanism includes a transparent fluid replenishment tank 3, the outer wall of the transparent fluid replenishment tank 3 is provided with scale lines, the inner wall of the incubator 1 is fixedly connected to the top of the transparent fluid replenishment tank 3, the inner wall of the transparent fluid replenishment tank 3 is slidably connected to a piston 4, and the bottom of the output end of the electric push rod 2 is fixedly connected to the top of the piston 4.

[0027] The outer wall of the incubator 1 is fixedly inlaid with a transparent acrylic plate 5, the bottom of the transparent fluid replenishment tank 3 is fixedly connected with a telescopic fluid replenishment tube 6, the outer wall of the telescopic fluid replenishment tube 6 is fixedly installed with a ball valve, the ball valve controls the opening and closing of the telescopic fluid replenishment tube 6, the electric push rod 2 drives the piston 4 downward, and the cell culture fluid in the transparent fluid replenishment tank 3 is injected into the telescopic fluid replenishment tube 6, the outer wall of the incubator 1 is hinged with a sealed door, and the interior of the incubator 1 is equipped with existing temperature and humidity control equipment.

[0028] The outer wall of the fan-shaped plate 12 is provided with an anti-skid mechanism, which includes multiple arc-shaped plates 15. Two circular rubber columns 13 are fixedly connected to the outer wall of the fan-shaped plate 12. The circular rubber columns 13 are squeezed with the partition 11. The outer wall of the fan-shaped plate 12 is fixedly inlaid with a silicone anti-skid pad 14. The silicone anti-skid pad 14 increases the anti-skid property of the culture vessel. The bottoms of the multiple arc-shaped plates 15 are respectively fixedly connected to the tops of the multiple fan-shaped plates 12. The arc-shaped plates 15 prevent the culture vessel from slipping out of the fan-shaped plate 12 when the disc 10 rotates.

[0029] Working principle: When in use, the incubator 1 is opened through the sealed door, and the culture utensils can be placed on multiple fan-shaped plates 12 respectively, separated by multiple partitions 11 to avoid crowding, so as to achieve the effect of comparison, and the sealed door is closed; when performing fluid replenishment, both hands are inserted into the incubator 1 through two silicone gloves 7, and there is no need to open the sealed door to operate, so as to reduce the interference of external factors. For culture bags or horizontal culture bottles, one end of one of the fan-shaped plates 12 is lifted, so that the two circular rubber columns 13 are rotated upward and squeezed on the partition 11, and the circular rubber columns 13 and the partition 11 are interposed between the circular rubber columns 13 and the partition 11. The friction force supports the fan-shaped plate 12 and the culture vessel, opens the ball valve and aligns the telescopic infusion tube 6 with the opening position of the culture vessel, and the electric push rod 2 drives the piston 4 downward to inject the cell culture fluid in the transparent infusion tank 3 into the telescopic infusion tube 6 and flow into the culture vessel. For the culture bottle, there is no need to rotate the fan-shaped plate 12. When the next round of culture vessels are infused with fluid, the motor 9 drives the disc 10 to rotate, and the rotation of the disc 10 drives multiple fan-shaped plates 12 to rotate, and multiple culture vessels are switched so that the fan-shaped plate 12 is located below the telescopic infusion tube 6.

[0030] The standard parts used in the utility model can all be purchased from the market, special-shaped parts can be customized according to the description in the specification and the drawings, the specific connection methods of each part all adopt the conventional means such as mature bolts, rivets, welding, etc. in the prior art, the machinery, parts and equipment all adopt the conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here, and the components known to technical personnel in this field, their structures and principles can be known to these technical personnel through technical manuals or through conventional experimental methods.

[0031] Although the embodiments of the present application have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present application, and that the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A fluid replenishment device, comprising an incubator (1), characterized in that: The top of the incubator (1) is fixedly connected to an electric push rod (2), a fluid replenishing mechanism is provided inside the incubator (1), two silicone gloves (7) are fixedly inlaid on the outer wall of the incubator (1), a chassis (8) is fixedly connected inside the incubator (1), a motor (9) is fixedly connected inside the chassis (8), a disc (10) is fixedly connected to the top of the output shaft of the motor (9), a plurality of partitions (11) are fixedly connected to the top of the disc (10), a plurality of fan-shaped plates (12) are hinged on the outer wall of the disc (10), and an anti-slip mechanism is provided on the outer wall of the fan-shaped plate (12).

2. A fluid replenishing device according to claim 1, characterized in that: The liquid replenishment mechanism comprises a transparent liquid replenishment box (3), the inner wall of the incubator (1) is fixedly connected to the top of the transparent liquid replenishment box (3), the inner wall of the transparent liquid replenishment box (3) is slidably connected to a piston (4), the bottom of the output end of the electric push rod (2) is fixedly connected to the top of the piston (4), and the bottom of the transparent liquid replenishment box (3) is fixedly connected to a telescopic liquid replenishment tube (6).

3. A fluid replenishing device according to claim 1, characterized in that: The anti-skid mechanism comprises a plurality of arc-shaped plates (15), the outer wall of the fan-shaped plate (12) is fixedly connected with two circular rubber columns (13), the circular rubber columns (13) are squeezed with the partition plate (11), the outer wall of the fan-shaped plate (12) is fixedly inlaid with a silicone anti-skid pad (14), and the bottoms of the plurality of arc-shaped plates (15) are respectively fixedly connected with the tops of the plurality of fan-shaped plates (12).

4. A fluid replenishing device according to claim 2, characterized in that: The tops of the incubator (1) and the transparent liquid replenishment tank (3) are both provided with through holes, and the inner wall of the through hole is slidably connected to the outer wall of the output end of the electric push rod (2).

5. A fluid replenishing device according to claim 1, characterized in that: The outer wall of the incubator (1) is fixedly inlaid with a transparent acrylic plate (5).

6. A fluid replenishing device according to claim 1, characterized in that: A circular hole is provided on the top of the chassis (8), and the inner wall of the circular hole is rotatably connected to the outer wall of the output shaft of the motor (9).

7. A fluid replenishing device according to claim 2, characterized in that: A ball valve is fixedly mounted on the outer wall of the telescopic liquid infusion tube (6), and a sealing door is hingedly mounted on the outer wall of the incubator (1).

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