Continuous fermentation tank for genetically engineered protein high-yield strain

Abstract

The utility model provides a continuous fermentation tank for genetic engineering protein high-yield strains, which belongs to the technical field of protein strain fermentation and comprises a fermentation tank body. The stirring and lifting assembly comprises a limiting rotating shaft rotationally connected to the top of the fermentation tank body, the outer wall of the limiting rotating shaft is slidably connected with a limiting sleeve, and the outer wall of the limiting sleeve is fixedly connected with a plurality of stirring blades. According to the utility model, fermentation liquor can be uniformly stirred and mixed by driving the plurality of stirring blades to rotate, and meanwhile, the plurality of stirring blades can be driven to move upwards, so that the stirring blades are prevented from occupying the fermentation space of strains, and the fermentation effect and the protein yield of the high-yield protein strains are ensured; fermentation products at the bottom of the fermentation tank body can be scraped by driving the cleaning scraper, so that the subsequent cleaning and maintenance work of the fermentation tank body is facilitated, and the subsequent fermentation work of high-yield protein strains is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of protein strain fermentation, in particular to a continuous fermentation tank for a genetic engineering protein high-yield strain. Background Art

[0002] A high-protein-yielding strain refers to a microbial strain that has been improved and optimized through genetic engineering or breeding. The high-protein-yielding strain has a high yield of specific proteins and can therefore be used for large-scale production of specific proteins. In the process of breeding genetically engineered strains for the production of recombinant protein thymosin-interferon fusion protein, the fermentation of the high-protein-yielding strain can fully test the protein production capacity of the strain.

[0003] The existing technology often has the following problems when used:

[0004] The fermentation liquid used for continuous fermentation of high-protein-yielding strains needs to be evenly mixed before use. If a stirring rod is directly arranged at the bottom of the fermentation tank, it will occupy part of the fermentation space of the strain, resulting in poor fermentation effect of the high-protein-yielding strain and affecting the protein yield of the strain. After the fermentation of the strain is completed, fermentation products and various wastes will exist at the bottom of the fermentation tank, making the cleaning and maintenance of the fermentation tank inconvenient and affecting the subsequent fermentation operations of the high-protein-yielding strain. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides a continuous fermentation tank for a genetically engineered protein high-yield strain.

[0006] The embodiment of the utility model provides a continuous fermentation tank for a genetically engineered protein high-yield strain, comprising:

[0007] Fermentation tank body;

[0008] A stirring lifting assembly, the stirring lifting assembly comprises a limit rotating shaft rotatably connected to the top of the fermentation tank body, the outer wall of the limit rotating shaft is slidably connected to a limit sleeve, the outer wall of the limit sleeve is fixedly connected to a plurality of stirring blades, and the top of the fermentation tank body is fixedly connected to a driving component;

[0009] The scraper assembly comprises two sliding brackets fixedly connected to the lower end of the outer wall of the limiting sleeve, the two sliding brackets are both slidably connected with straight rods, and the lower ends of the two straight rods are fixedly connected with cleaning scrapers.

[0010] Furthermore, the driving component includes a rotating motor fixedly connected to the top of the fermentation tank body, the output end of the rotating motor is fixedly connected to the upper end of the limiting rotating shaft, the top surface of the fermentation tank body is rotatably connected to a bearing through a rotating bracket, two electric lifting rods are fixedly connected to the inner side of the bearing, and two adapter blocks are fixedly connected to the upper end of the outer wall of the limiting sleeve, and the output ends of the two electric lifting rods are respectively fixedly connected to the two adapter blocks.

[0011] Furthermore, a protrusion is fixedly connected to the outer wall of the limiting rotating shaft, and a groove is formed on the inner wall of the limiting sleeve, and the protrusion matches the groove.

[0012] Furthermore, a collar is fixedly connected to the outer wall of the straight rod, and a compression spring is fixedly connected between the collar and the sliding bracket.

[0013] Furthermore, a feed inlet is provided at the top of the fermentation tank body, a discharge port is provided at the lower end of the side wall of the fermentation tank body, and a closed cover plate is movably connected to the feed inlet and the discharge port.

[0014] Furthermore, a reinforcement base is fixedly connected to the bottom of the fermentation tank body.

[0015] Compared with the prior art, the utility model has the following beneficial effects:

[0016] The utility model is provided with a stirring lifting assembly, which drives a plurality of stirring blades to rotate by driving a limit rotating shaft and a limit sleeve, and utilizes the rotating plurality of stirring blades to evenly stir and mix the fermentation liquid, and can drive the limit sleeve to move upward relative to the limit rotating shaft, so as to avoid the stirring blades occupying the fermentation space of the strain, thereby ensuring the fermentation effect and protein yield of the high-yield protein strain;

[0017] The utility model provides a scraper assembly, which can drive the cleaning scraper to rotate relative to the inner bottom surface of the fermentation tank body after the fermentation of the strain is completed, so that the fermentation product at the bottom of the fermentation tank body can be scraped off by the rotating cleaning scraper, thereby facilitating the subsequent cleaning and maintenance work of the fermentation tank body, which is beneficial to the subsequent fermentation operation of the high-protein yield strain. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the three-dimensional structure of a continuous fermentation tank for a genetically engineered protein high-yielding strain described in an embodiment of the utility model.

[0019] Figure 2 This is a schematic diagram of the internal structure of a continuous fermentation tank for a genetically engineered protein high-yielding strain described in an embodiment of the utility model.

[0020] Figure 3 for Figure 2 Enlarged view of point A.

[0021] Figure 4 It is an exploded view of the structure of the limiting rotating shaft and the limiting sleeve part in a continuous fermentation tank for a genetically engineered protein high-yielding strain described in an embodiment of the utility model.

[0022] In the above drawings: 1 fermentation tank body, 2 limiting rotating shaft, 3 limiting sleeve, 4 stirring blade, 5 sliding bracket, 6 straight rod, 7 cleaning scraper, 8 rotating motor, 9 bearing, 10 electric lifting rod, 11 protrusion, 12 compression spring, 13 feed port, 14 discharge port, 15 reinforcement base. DETAILED DESCRIPTION

[0023] The technical solution of the present utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0024] like Figure 1-Figure 4 As shown, the embodiment of the utility model proposes a continuous fermentation tank for high-yield strains of genetically engineered protein, including a fermentation tank body 1, a stirring and lifting assembly and a scraping assembly: a feed inlet 13 is provided at the top of the fermentation tank body 1, a discharge port 14 is provided at the lower end of the side wall of the fermentation tank body 1, and a closed cover plate is movably connected at the feed inlet 13 and the discharge port 14, and a reinforcement base 15 is fixedly connected at the bottom of the fermentation tank body 1; the stirring and lifting assembly includes a limit shaft 2 rotatably connected to the top of the fermentation tank body 1, a limit sleeve 3 is slidably connected to the outer wall of the limit shaft 2, a protrusion 11 is fixedly connected to the outer wall of the limit shaft 2, a groove is provided on the inner wall of the limit sleeve 3, the protrusion 11 matches the groove, a plurality of stirring blades 4 are fixedly connected to the outer wall of the limit sleeve 3, and a driving component is fixedly connected to the top of the fermentation tank body 1, and the driving component includes a rotating motor 8 fixedly connected to the top of the fermentation tank body 1, and the output end of the rotating motor 8 is fixedly connected to the upper end of the limit shaft 2;

[0025] The limiting rotating shaft 2 and the limiting sleeve 3 are driven to rotate by the rotating motor 8, so that the stirring blades 4 on the outer wall of the limiting sleeve 3 start to rotate, and the fermentation mixture is fully stirred by the rotating stirring blades 4 so that the fermentation liquid can be evenly mixed.

[0026] like Figure 2 and Figure 4 As shown, the inner top surface of the fermentation tank body 1 is rotatably connected to a bearing 9 through a rotating bracket, two electric lifting rods 10 are fixedly connected to the inner side of the bearing 9, and two adapter blocks are fixedly connected to the upper end of the outer wall of the limiting sleeve 3, and the output ends of the two electric lifting rods 10 are respectively fixedly connected to the two adapter blocks;

[0027] When the rotating motor 8 drives the limit shaft 2 and the limit sleeve 3 to rotate, the two electric lifting rods 10 inside the bearing 9 can rotate synchronously, so as to control the rotation and lifting movement of the limit sleeve 3 through the electric lifting rods 10;

[0028] By controlling the two electric lifting rods 10 to drive the limit sleeve 3 to move upward, the limit sleeve 3 can move upward relative to the limit shaft 2, thereby allowing a number of stirring blades 4 to move above the fermentation liquid, thereby preventing the stirring blades 4 from occupying the fermentation space of the strain, thereby ensuring the fermentation effect and protein yield of the high-protein yield strain.

[0029] like Figure 2 and Figure 3 As shown, the scraper assembly includes two sliding brackets 5 fixedly connected to the lower end of the outer wall of the limiting sleeve 3, and the two sliding brackets 5 are slidably connected with straight rods 6. The lower ends of the two straight rods 6 are fixedly connected with cleaning scrapers 7, and the outer wall of the straight rod 6 is fixedly connected with a ring, and a compression spring 12 is fixedly connected between the ring and the sliding bracket 5.

[0030] The limiting rotating shaft 2 and the limiting sleeve 3 are driven to rotate by rotating the motor 8, and the limiting sleeve 3 is driven to move down to a suitable position by controlling the electric lifting rod 10. At this position, the compression spring 12 is in a compressed and force-storing state. The compression spring 12 can elastically push the cleaning scraper 7, so that the cleaning scraper 7 can be fully rotated relative to the inner bottom surface of the fermentation tank body 1, so that the fermentation product at the bottom of the fermentation tank body 1 can be scraped off by the rotating cleaning scraper 7, thereby facilitating the subsequent cleaning and maintenance work of the fermentation tank body 1, and is beneficial to the subsequent fermentation operation of the high-protein-yielding strain.

[0031] The detailed working process of the utility model is as follows:

[0032] 1. When in use, the fermentation mixture is introduced into the fermentation tank body 1 through the feed port 13, and the rotary motor 8 is controlled to start, and the limiting rotating shaft 2 and the limiting sleeve 3 are driven to rotate by the rotary motor 8, so that the plurality of stirring blades 4 on the outer wall of the limiting sleeve 3 start to rotate, and the fermentation mixture is fully stirred by the rotating plurality of stirring blades 4, so that the fermentation liquid can be evenly mixed;

[0033] 2. After the fermentation liquid is stirred and mixed, the two electric lifting rods 10 can be controlled to drive the limit sleeve 3 to move upward, so that the limit sleeve 3 can move upward relative to the limit rotating shaft 2, and then the plurality of stirring blades 4 can be moved to the top of the fermentation liquid, thereby preventing the stirring blades 4 from occupying the fermentation space of the strain, thereby ensuring the fermentation effect and protein yield of the high-yield protein strain;

[0034] 3. After the fermentation of the strain is completed, the limit shaft 2 and the limit sleeve 3 can also be driven to rotate. The limit sleeve 3 can be driven to move down to a suitable position by controlling the electric lifting rod 10. At this position, the compression spring 12 is in a compressed and force-storing state. The compression spring 12 can be used to elastically push the cleaning scraper 7, so that the cleaning scraper 7 can be fully rotated relative to the inner bottom surface of the fermentation tank body 1, so that the fermentation product at the bottom of the fermentation tank body 1 can be scraped off by the rotating cleaning scraper 7, thereby facilitating the subsequent cleaning and maintenance work of the fermentation tank body 1, which is beneficial to the subsequent fermentation operation of the high-protein-yielding strain.

[0035] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the utility model, which should be included in the scope of the claims of the utility model.

Claims

1. A continuous fermentation tank for a genetically engineered protein high-yield strain, characterized in that: include: Fermentation tank body (1); A stirring lifting assembly, the stirring lifting assembly comprising a limit rotating shaft (2) rotatably connected to the top of a fermentation tank body (1), a limit sleeve (3) slidably connected to the outer wall of the limit rotating shaft (2), a plurality of stirring blades (4) fixedly connected to the outer wall of the limit sleeve (3), and a driving component fixedly connected to the top of the fermentation tank body (1); A scraper assembly, the scraper assembly comprising two sliding brackets (5) fixedly connected to the lower end of the outer wall of the limiting sleeve (3), the two sliding brackets (5) are both slidably connected to a straight rod (6), and the lower ends of the two straight rods (6) are fixedly connected to a cleaning scraper (7).

2. The continuous fermentation tank for a genetically engineered protein high-yielding strain according to claim 1, characterized in that: in: The driving component comprises a rotating motor (8) fixedly connected to the top of the fermentation tank body (1); the output end of the rotating motor (8) is fixedly connected to the upper end of the limiting rotating shaft (2); the inner top surface of the fermentation tank body (1) is rotatably connected to a bearing (9) via a rotating bracket; two electric lifting rods (10) are fixedly connected to the inner side of the bearing (9); the upper end of the outer wall of the limiting sleeve (3) is fixedly connected to two adapter blocks; the output ends of the two electric lifting rods (10) are respectively fixedly connected to the two adapter blocks.

3. The continuous fermentation tank for a genetically engineered protein high-yielding strain according to claim 1, characterized in that: in: A protrusion (11) is fixedly connected to the outer wall of the limiting rotating shaft (2), and a groove is formed on the inner wall of the limiting sleeve (3), and the protrusion (11) matches the groove.

4. The continuous fermentation tank for a genetically engineered protein high-yielding strain according to claim 1, characterized in that: in: A collar is fixedly connected to the outer wall of the straight rod (6), and a compression spring (12) is fixedly connected between the collar and the sliding bracket (5).

5. The continuous fermentation tank for a genetically engineered protein high-yielding strain according to claim 1, characterized in that: in: The top of the fermentation tank body (1) is provided with a feed inlet (13), and the lower end of the side wall of the fermentation tank body (1) is provided with a discharge port (14), and the feed inlet (13) and the discharge port (14) are both movably connected with a closed cover plate.

6. The continuous fermentation tank for a genetically engineered protein high-yielding strain according to claim 1, characterized in that: in: A reinforcement base (15) is fixedly connected to the bottom of the fermentation tank body (1).

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