Micro-fluidic chip
By designing a microfluidic chip including injection area, reaction area, mixing area and detection area, the detection problem of professional equipment and environment in the prior art is solved, and simple and rapid pathogenic microbial detection is realized in non-laboratory environments, which is suitable for home-style and field environment detection.
Patent Information
- Application Number
- CN202420854756.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-23
- Publication Date
- 2025-05-16
- Estimated Expiration
- 2034-04-23
AI Technical Summary
The existing detection methods that combine ring-mediated isothermal amplification technology with microfluidic chips require professional equipment and laboratory environments, and cannot conduct simple and rapid pathogenic microbial testing in non-laboratory environments, and require professional operations, so home-based testing cannot be achieved.
A microfluidic chip is designed, including a chip cover plate and a chip substrate, and is equipped with a sample injection area, a reaction area, a mixing area and a detection area. The overall structure is simple and has a high degree of integration, so it can be tested without the need for professional laboratory environment and professional training.
It realizes simple and rapid pathogenic microbial testing in a non-laboratory environment, which is suitable for ordinary people to conduct home-based testing and in the absence of professional personnel and equipment, improving the convenience and usability of testing.
Smart Images
Figure CN222877915U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microfluidics, in particular to a microfluidics chip. Background Art
[0002] Loop-mediated isothermal amplification (LMAP) is a commonly used nucleic acid amplification technology that can quickly and efficiently amplify target DNA or RNA sequences under constant temperature conditions. The basic principle of LMAP is to combine multiple primers and special DNA polymerases to cause continuous DNA synthesis reactions on the target sequence to form characteristic ring structures. These ring structures can be continuously generated during the reaction and can be visualized after the amplification reaction. Compared with traditional polymerase chain reaction, it has the following characteristics: isothermal reaction, rapid and efficient, high specificity and direct visualization.
[0003] Deficiencies of existing technology:
[0004] Currently, many studies have combined loop-mediated isothermal amplification technology with microfluidic chips to achieve simple and rapid detection of pathogenic microorganisms. However, this research still requires a variety of auxiliary equipment such as imports and water-soluble pots to provide constant temperature. It cannot be tested in a non-laboratory environment and requires professional operation, which cannot achieve home testing. Utility Model Content
[0005] The purpose of the utility model is to provide a microfluidic chip to solve the problems raised in the above background technology.
[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a microfluidic chip, comprising a chip cover plate and a chip substrate, the chip cover plate is provided with a first upper surface and a first lower surface, the chip substrate is provided with a second upper surface and a second lower surface, the first lower surface and the second upper surface are bonded to each other, and the length direction, width direction and height direction of the chip cover plate and the chip substrate are respectively a first direction, a second direction and a third direction; the chip cover plate is provided with a first sample injection area, and the chip substrate is provided with a second sample injection area, a reaction area, a mixing area and a detection area.
[0007] Preferably, the first sample introduction area includes a first pool groove and a first circular groove group, the first pool groove is arranged on the first upper surface, the first circular groove group is arranged on the first lower surface, the first circular groove group includes three upper circular grooves, the three upper circular grooves are arranged at equal intervals in the second direction and installed on the first lower surface; the second sample introduction area is a second circular groove group, the second circular groove group includes three lower circular grooves, the three lower circular grooves are arranged at equal intervals in the second direction and installed on the second upper surface, the three upper circular grooves and the three lower circular grooves are of the same size and are aligned in the third direction.
[0008] Preferably, the reaction zone is a second tank group, which includes three reaction tanks, which are arranged at equal intervals in the second direction and installed on the second upper surface, and the three reaction tanks are connected to the three lower circular tanks through straight grooves; a temperature control module is installed at the bottom of the second tank group.
[0009] Preferably, the mixing zone includes a third circular groove and a curved groove, the third circular groove and the curved groove are arranged in the first direction and are both installed on the second upper surface, the third circular groove and the second pool groove group are connected through the straight groove, and the curved groove and the third circular groove are connected through the straight groove.
[0010] Preferably, the detection area includes a fourth circular groove and a third pool groove, the fourth circular groove and the third pool groove are installed on the second upper surface, and the fourth circular groove and the curved groove are connected through the straight groove.
[0011] Preferably, a through hole is provided on the second lower surface.
[0012] Preferably, the first direction, the second direction and the third direction are perpendicular to each other.
[0013] Compared with the prior art, the beneficial effects of the utility model are:
[0014] 1. This microfluidic chip is provided with a first sample injection area, a second sample injection area, a reaction area, a mixing area and a detection area. The liquid to be tested enters the mixing area after being heated in the reaction area, and finally enters the detection area. The overall device has a simple structure, high integration, and requires less detection liquid. It does not require a professional laboratory environment or professional training. It is simple to operate and is suitable for ordinary people to conduct home-based testing, as well as testing in some field environments or under conditions where there is a lack of professionals and equipment.
[0015] 2. This microfluidic chip is provided with a through hole, and the detected waste liquid is discharged to an external device through the through hole. A pumping and pressure device can also be installed at the through hole. After the detected liquid enters the chip, the reaction of the liquid is controlled by pumping and pressure. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0017] Figure 2 It is an exploded schematic diagram of the overall structure of the utility model;
[0018] Figure 3 This is a specific schematic diagram of the chip substrate of the utility model;
[0019] In the figure: 110, chip cover plate; 120, chip substrate; 111, first upper surface; 112, first lower surface; 121, second upper surface; 122, second lower surface; x, first direction; y, second direction; z, third direction; 131, first pool groove; 132, first circular groove group; 1321, three upper circular grooves; 133, second circular groove group; 1331, three lower circular grooves; 141, second pool groove group; 1411, three reaction grooves; 150, straight groove; 161, third circular groove; 162, curved groove; 171, fourth circular groove; 172, third pool groove; 180, through hole. DETAILED DESCRIPTION
[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0021] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicating orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0022] In the description of this patent, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "setting" should be understood in a broad sense, for example, it can be fixed connection, setting, or detachable connection, setting, or integrated connection, setting. For ordinary technicians in this field, the specific meanings of the above terms in this patent can be understood according to specific circumstances.
[0023] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present utility model, the meaning of "several" is two or more, unless otherwise clearly and specifically defined.
[0024] Example
[0025] See also Figure 1-3As shown, the utility model provides a microfluidic chip technical solution: including a chip cover plate 110 and a chip substrate 120, the chip cover plate 110 and the chip substrate 120 are equal in size, the chip cover plate 110 is provided with a first upper surface 111 and a first lower surface 112, the chip substrate 120 is provided with a second upper surface 121 and a second lower surface 122, the first lower surface 112 of the chip cover plate 110 and the second upper surface 121 of the chip substrate 120 are bonded by a film, and the length direction, width direction and height direction of the chip cover plate 110 and the chip substrate 120 are set to be a first direction x, a second direction y and a third direction z, respectively, and the first direction x, the second direction y and the third direction z are respectively Two vertical; the chip cover plate 110 is provided with a first sample inlet area, and the chip substrate 120 is provided with a first sample inlet area, a second positive inlet area, a reaction area, a mixing area and a detection area; during operation, the first lower surface 112 and the second upper surface 121 are bonded by a film, and the liquid to be tested is introduced into the first sample inlet area, the liquid to be tested enters the second sample inlet area, and then flows to the reaction area. The liquid to be tested enters the mixing area after the reaction is heated, and finally enters the detection area. The overall device has a simple structure, high integration, and requires less detection liquid. It does not require a professional laboratory environment or professional training. It is simple to operate and is suitable for ordinary people to conduct home-based testing, as well as testing in some field environments or under conditions where there is a lack of professionals and equipment.
[0026] Furthermore, the first sample introduction area includes a first pool groove 131 and a first circular groove group 132, the first pool groove 131 is arranged on the first upper surface 111, the first circular groove group 132 is arranged on the first lower surface 112, the first circular groove group 132 includes three upper circular grooves 1321, the three upper circular grooves 1321 are arranged at equal intervals in the second direction y and are installed on the first lower surface 112, and the three upper circular grooves 1321 are communicated with the first pool groove 131; the second sample introduction area is a second circular groove group 133, the second circular groove group 133 includes three lower circular grooves 1331, the three lower circular grooves 1331 are arranged at equal intervals in the second direction y and are installed on the second upper surface 121, the three lower circular grooves 1331 and the three upper circular grooves 1321 are of the same size and are aligned and fitted in sequence in the third direction z. The first pool 131 covers the first circular groove group 132 and the second circular groove group 133, so that the chip cover 110 and the chip substrate 120 are connected and the first circular groove group 132 and the second circular groove group 133 can be distributed to the object to be tested simultaneously and evenly. The liquid to be tested is added to the first injection area and flows into the second injection area, and enters the three upper circular grooves 1321 from the first pool 131. The three upper circular grooves 1321 divide the liquid to be tested into three parts, and flow to the three lower circular grooves 1331 of the chip substrate 120 respectively. There is no need to add the test liquid multiple times or add the test liquid to multiple reagent tubes. Only one injection operation is required to achieve simultaneous amplification of multiple targets, which simplifies the operation process of the staff and greatly improves convenience and usability.
[0027] Furthermore, the reaction zone is a second pool group 141, and the second pool group 141 includes three reaction grooves 1411. The three reaction grooves 1411 are arranged at equal intervals in the second direction y and installed on the second upper surface 121. The three reaction grooves 1411 are connected to the three lower circular grooves 1331 through the straight groove 150, and a temperature control module is installed at the bottom of the second pool group 141. The liquid to be tested flows from the first sampling area into the second sampling area and then into the reaction area. Before the first lower surface 112 and the second upper surface 121 are bonded, the reaction enzyme is stored in the three reaction grooves 1411, so that the liquid to be tested reacts with the enzyme, and the liquid to be tested is heated to a preset temperature by the installed temperature control module. The preset temperature range is 60°C to 67°C, which reduces the overall space size, makes the chip structure simpler, and is more convenient to manufacture.
[0028] Furthermore, the mixing zone includes a third circular groove 161 and a curved groove 162, the third circular groove 161 and the curved groove 162 are arranged in the first direction x and are both installed on the second upper surface 121, the third circular groove 161 is connected to the second pool group 141 through the straight groove 150, and the curved groove 162 and the third circular groove 161 are connected through the straight groove 150. When the liquid to be tested is heated to a set temperature in the reaction zone, the liquid to be tested in the three reaction grooves 1411 is introduced into the third circular groove 161 for mixing through an external driving device, and then enters the curved groove 162 for mixing, so that the mixing is more complete.
[0029] Furthermore, the detection area includes a fourth circular groove 171 and a third pool groove 172, and the fourth circular groove 171 and the third pool groove 172 are installed on the second upper surface 121, and the fourth circular groove 171 and the curved groove 162 are connected by a straight groove 150. When the liquid to be tested enters the mixing area and is evenly mixed, it will enter the fourth circular groove 171 for detection, and the third pool groove 172 stores the detection test paper.
[0030] Furthermore, a through hole 180 is provided on the second lower surface 122 , and the detected waste liquid is discharged to an external device through the through hole 180 .
[0031] Furthermore, the test method is that the test paper adopts the colloidal gold double antibody sandwich method, the test strip uses Fam monoclonal antibody and chicken IgY antibody for colloidal gold labeling and capture, the detection line is coated with Biotin (T3) / TAMRA (T2) / DIG (T1) monoclonal antibody, and the quality control line is coated with sheep anti-chicken IgY (C) antibody. When the sample to be tested contains Fam and Biotin / TAMRA / DIG modified target detection objects, they will bind to the gold-labeled antibody and then bind to the antibody on the NC membrane to form a gold-labeled antibody-target detection object-antibody complex, thereby displaying a purple-red band at the detection line, and negative samples will only display a purple-red band at the quality control line (C).
[0032] Furthermore, the chip cover plate 110 and the chip substrate 120 are both made of polymethyl methacrylate.
[0033] Furthermore, the chip can be driven by extrusion and pumping. An extrusion piece is installed at the first tank 131. After the measured liquid enters the first tank 131, the reaction of the liquid is controlled by extrusion. The through hole 180 ensures that the flow of the measured liquid will not be obstructed during the extrusion process. In addition, a pumping device can be installed at the through hole 180 of the second lower surface 122 of the chip substrate 120. After the measured liquid enters the chip, the reaction of the liquid is controlled by pumping.
[0034] The working principle of the utility model is as follows:
[0035] When a microfluidic chip of the present embodiment is in use, the chip cover plate 110 is provided with a first sample inlet area, and the chip substrate 120 is provided with a first sample inlet area, a second positive inlet area, a reaction area, a mixing area and a detection area; during operation, the first lower surface 112 and the second upper surface 121 are bonded by a film, and the liquid to be tested is introduced into the first sample inlet area, the liquid to be tested enters the second sample inlet area, and then flows to the reaction area. The liquid to be tested enters the mixing area after being heated for reaction, and finally enters the detection area. The overall device has a simple structure, high integration, and requires less detection liquid. It does not require a professional laboratory environment or professional training. It is simple to operate and is suitable for ordinary people to perform home-based testing, as well as testing in some field environments or under conditions where there is a lack of professionals and equipment.
[0036] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.
Claims
1. A microfluidic chip, comprising a chip cover plate (110) and a chip substrate (120), characterized in that: The chip cover plate (110) is provided with a first upper surface (111) and a first lower surface (112); the chip substrate (120) is provided with a second upper surface (121) and a second lower surface (122); the first lower surface (112) and the second upper surface (121) are bonded to each other; the length direction, width direction and height direction of the chip cover plate (110) and the chip substrate (120) are respectively a first direction (x), a second direction (y) and a third direction (z); the chip cover plate (110) is provided with a first sample injection area; the chip substrate (120) is provided with a second sample injection area, a reaction area, a mixing area and a detection area; The detection area comprises a fourth circular groove (171) and a third pool groove (172), and the fourth circular groove (171) and the third pool groove (172) are installed on the second upper surface (121); A through hole (180) is provided on the second lower surface (122).
2. A microfluidic chip according to claim 1, characterized in that: The first sample introduction area includes a first pool groove (131) and a first circular groove group (132), the first pool groove (131) is arranged on the first upper surface (111), the first circular groove group (132) is arranged on the first lower surface (112), the first circular groove group (132) includes three upper circular grooves (1321), the three upper circular grooves (1321) are arranged at equal intervals in the second direction (y) and are installed on the first lower surface (112); the second sample introduction area is a second circular groove group (133), the second circular groove group (133) includes three lower circular grooves (1331), the three lower circular grooves (1331) are arranged at equal intervals in the second direction (y) and are installed on the second upper surface (121), the three upper circular grooves (1321) and the three lower circular grooves (1331) are of the same size and are aligned in the third direction (z).
3. A microfluidic chip according to claim 2, characterized in that: The reaction zone is a second tank group (141), and the second tank group (141) includes three reaction tanks (1411). The three reaction tanks (1411) are arranged at equal intervals in the second direction (y) and installed on the second upper surface (121). The three reaction tanks (1411) are connected to the three lower circular grooves (1331) through straight grooves (150); a temperature control module is installed at the bottom of the second tank group (141).
4. A microfluidic chip according to claim 3, characterized in that: The mixing zone includes a third circular groove (161) and a curved groove (162); the third circular groove (161) and the curved groove (162) are arranged in the first direction (x) and are both installed on the second upper surface (121); the third circular groove (161) and the second pool group (141) are connected via the straight groove (150); and the curved groove (162) and the third circular groove (161) are connected via the straight groove (150).
5. A microfluidic chip according to claim 4, characterized in that: The fourth circular groove (171) and the curved groove (162) are connected via the straight groove (150).
6. A microfluidic chip according to claim 1, characterized in that: The first direction (x), the second direction (y) and the third direction (z) are perpendicular to each other.