Rotary uniform-mixing automatic nucleic acid extractor

Abstract

The utility model discloses a rotary uniform-mixing automatic nucleic acid extraction instrument, which comprises a vertical moving assembly comprising a magnetic bar sleeve moving assembly, a magnetic bar moving assembly and a vertical bracket for supporting the magnetic bar sleeve moving assembly and the magnetic bar moving assembly; the rotary uniform mixing assembly comprises a magnetic rod assembly provided with a magnetic rod and a magnetic rod sleeve rotating assembly provided with a magnetic rod sleeve which are arranged up and down; the magnetic bar sleeve moving assembly is connected with the magnetic bar sleeve rotating assembly and drives the magnetic bar sleeve to move downwards and stretch into the deep hole plate. The magnetic bar moving assembly is connected with the magnetic bar assembly and drives the magnetic bar to move downwards and stretch into the magnetic bar sleeve so that the magnetic beads in the deep hole plate can be attracted to the magnetic bar sleeve. The magnetic rod sleeve rotating assembly drives the magnetic rod sleeve to rotate so as to uniformly mix a nucleic acid extraction sample in the deep hole plate; the nucleic acid extraction instrument is good in uniform mixing effect, does not generate noise and bubbles, and adopts multiple channels to improve the nucleic acid extraction efficiency.

Description

Technical Field

[0001] The utility model belongs to the technical field of biomedical equipment, in particular to an automatic nucleic acid extraction instrument for rotating and mixing. Background Art

[0002] In the existing nucleic acid extraction equipment, for the magnetic bead mixing method, most of them adopt up and down shaking mixing or pipette blowing mixing. Up and down shaking mixing causes too much vibration and noise, and pipette blowing mixing is prone to bubbles, which affects the final extraction effect.

[0003] In addition, most designs are 8-channel, with low throughput of single nucleic acid extraction and low efficiency of nucleic acid extraction. Utility Model Content

[0004] The utility model is dedicated to solving the problems raised in the above background technology, and provides a linear rotary motor with large thrust and high precision, and has a compact structure and a small size.

[0005] One aspect of the utility model provides a rotary mixing automatic nucleic acid extractor, which has a good mixing effect and does not generate noise and bubbles.

[0006] The utility model discloses a rotating and mixing automatic nucleic acid extractor, which comprises:

[0007] A vertical moving assembly, comprising a magnetic rod sleeve moving assembly, a magnetic rod moving assembly and a vertical bracket supporting the two;

[0008] The rotating mixing component comprises a magnetic rod component provided with magnetic rods and a magnetic rod sleeve rotating component provided with magnetic rod sleeves arranged above and below; the magnetic rod sleeve moving component is connected to the magnetic rod sleeve rotating component and drives the magnetic rod sleeve to move downward and extend into the deep-well plate; the magnetic rod moving component is connected to the magnetic rod component and drives the magnetic rod to move downward and extend into the magnetic rod sleeve to adsorb the magnetic beads in the deep-well plate on the magnetic rod sleeve; the magnetic rod sleeve rotating component drives the magnetic rod sleeve to rotate and mix the nucleic acid extraction sample in the deep-well plate;

[0009] A heating component is arranged at the lower part of the deep well plate, and heats the nucleic acid extraction sample in the deep well plate during the nucleic acid extraction process to meet the nucleic acid extraction conditions;

[0010] A horizontal moving component drives the vertical moving component and the rotating mixing component to move horizontally to complete all steps of nucleic acid extraction;

[0011] A bottom plate supports the rotating mixing component, the vertical moving component, the horizontal moving component and the heating component.

[0012] Preferably, the magnetic rod sleeve rotating assembly comprises:

[0013] A first drive motor, the output shaft of which is arranged vertically downward;

[0014] A first primary gear connected to an output shaft of the first driving motor;

[0015] The first secondary gear and the second secondary gear are both meshed with the first primary gear;

[0016] A first row of three-stage gears and a second row of three-stage gears are arranged in parallel, the first secondary gear is meshed with one of the three-stage gears in the first row of three-stage gears, the second secondary gear is meshed with one of the three-stage gears in the second row of three-stage gears, and two adjacent three-stage gears in the first row of three-stage gears and the second row of three-stage gears are meshed, and the center of each of the three-stage gears is sleeved with the magnetic rod sleeve;

[0017] A magnetic rod sleeve support plate supports the first drive motor and the magnetic rod sleeve, and the magnetic rod sleeve support plate is connected to the magnetic rod sleeve moving assembly.

[0018] Preferably, it also includes:

[0019] a second primary gear, a third secondary gear and a fourth secondary gear, wherein the second primary gear is meshed with the third secondary gear and the fourth secondary gear, and the third secondary gear is meshed with one of the third-stage gears in the second row of third-stage gears;

[0020] The third row of three-stage gears is parallel to the second row of three-stage gears, and one of the three-stage gears in the third row is meshed with the fourth secondary gear; two adjacent three-stage gears in the third row are meshed, and the center of each of the three-stage gears in the third row is sleeved with the magnetic rod sleeve.

[0021] Preferably, the first, second and third rows of three-stage gears are each provided with 8 three-stage gears.

[0022] Preferably, the magnetic rod sleeve moving assembly includes a second drive motor and a first linear drive connected to the second drive motor, and the first linear drive is connected to the magnetic rod sleeve support plate.

[0023] Preferably, the first linear drive is a first nut-screw mechanism, which includes a first screw connected to the second drive motor and a first nut sleeved on the first screw, and the first nut is fixedly connected to the magnetic rod sleeve support plate.

[0024] Preferably, the magnetic bar moving assembly includes a third drive motor and a second linear drive connected to the third drive motor;

[0025] The magnetic bar assembly comprises a magnetic bar support and a plurality of magnetic bars arranged in parallel on the magnetic bar support;

[0026] The second linear drive is connected to the magnetic rod support.

[0027] Preferably, the second linear drive is a second nut-screw mechanism, the second nut-screw mechanism includes a second screw and a second nut sleeved on the second screw, and the second nut is fixedly connected to the magnetic rod support.

[0028] Preferably, the vertical movement assembly further comprises a slide rail assembly, which comprises a double slide rail and four sliders cooperating with the double slide rail;

[0029] The magnetic rod sleeve moving assembly further comprises a first connecting plate fixedly connected to the two sliding blocks, and the first connecting plate is fixedly connected to the magnetic rod sleeve supporting plate;

[0030] The magnetic bar moving assembly also includes a second connecting plate fixedly connected to the two sliding blocks, and the second connecting plate is fixedly connected to the magnetic bar bracket.

[0031] Preferably, the horizontal moving component includes a fourth drive motor with an output shaft horizontally arranged on the base plate, an active synchronous wheel and a driven synchronous wheel coaxially connected to the output shaft of the fourth drive motor, and a synchronous belt sleeved on the active synchronous wheel and the driven synchronous wheel, and the synchronous belt is fixedly connected to the vertical bracket.

[0032] Preferably, the horizontal moving assembly includes a fourth drive motor with an output shaft horizontally arranged on the base plate, a driving sprocket and a driven sprocket coaxially connected to the output shaft of the fourth drive motor, and a chain sleeved on the driving sprocket and the driven sprocket, and the chain is fixedly connected to the vertical bracket.

[0033] Preferably, the heating assembly includes a heating rod disposed at the bottom of the deep well plate, a heating support plate supporting the heating rod, and a temperature control plate disposed below the heating support plate for controlling the temperature of the heating rod.

[0034] By adopting the above technical solution, the utility model has the following beneficial effects compared with the prior art:

[0035] The utility model rotary mixing automatic nucleic acid extractor rotates the magnetic rod sleeve 125 extending from the first row of holes to the last row of holes of the deep-well plate with a vertical axis as the rotation axis, so that the lysis solution and the nucleic acid extraction sample in the first row of holes of the deep-well plate are evenly mixed, the lysis rate is increased, and the nucleic acid extraction rate is high; the rotation in the last row of holes of the deep-well plate allows the elution solution to fully contact with the magnetic beads, and the nucleic acid molecules on the magnetic beads are completely eluted, thereby improving the elution rate; the rotation in the middle hole of the deep-well plate allows the cleaning solution to fully contact with the magnetic beads, so that the nucleic acid molecules on the magnetic beads are cleaned more cleanly.

[0036] In addition, the utility model rotary mixing automatic nucleic acid extractor adopts 3 rows of magnetic rods and 3 rows of magnetic rod covers, each row is provided with 8 magnetic rods and magnetic rod covers, forming a total of 24 channels for simultaneous nucleic acid extraction, which greatly improves the efficiency of nucleic acid extraction.

[0037] These and other features, aspects and advantages of the present application will become better understood with reference to the following description.The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the present application and, together with the description, serve to explain the principles of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] This specification describes the complete and instructive disclosure of the present application, including its best mode of implementation, for those skilled in the art. This specification refers to the accompanying drawings, in which:

[0039] Figure 1 One of the structural schematic diagrams of a rotary mixing automatic nucleic acid extractor provided in an embodiment of the utility model;

[0040] Figure 2 A schematic diagram of the structure of a rotating mixing component of an automatic nucleic acid extractor for rotating mixing provided by an embodiment of the utility model;

[0041] Figure 3 A schematic structural diagram of a magnetic rod sleeve rotating assembly of an existing rotating and mixing automatic nucleic acid extractor provided in an embodiment of the utility model;

[0042] Figure 4 A schematic structural diagram of a vertical moving assembly of an existing rotary mixing automatic nucleic acid extractor provided in an embodiment of the utility model;

[0043] Figure 5 The second structural schematic diagram of an existing rotary mixing automatic nucleic acid extractor provided in an embodiment of the utility model. DETAILED DESCRIPTION

[0044] Now with reference to the embodiments of the present application in detail, one or more examples of the embodiments of the present application are illustrated in the drawings. Each example is provided to explain the present application, not to limit the present application. In fact, it will be clear to those skilled in the art that various modifications and changes can be made in the present application without departing from the scope or spirit of the present application. For example, the features described or described as a part of an embodiment can be used together with another embodiment to produce another embodiment. Therefore, it is hoped that the present application covers such modifications and changes, which are within the scope of the attached claims and their equivalents. As used in this specification, the terms "first", "second", etc. are used interchangeably to distinguish one component from another component without intending to indicate the position or importance of each component. As used in the specification, unless the context clearly indicates otherwise, the terms "one", "an", "the" and "said" are intended to indicate that there are one or more elements. The terms "include", "include" and "have" are intended to be inclusive and mean that there may be other elements in addition to the listed elements.

[0045] Now referring to the drawings, in which the same numerals represent the same elements in all the drawings, the present invention will be further explained below in conjunction with specific embodiments.

[0046] The utility model provides a rotary mixing automatic nucleic acid extractor with good mixing effect, low noise, no bubbles in the mixing process, multiple mixing channels and high nucleic acid extraction efficiency.

[0047] like Figure 1 As shown, it is a structural diagram of a rotary mixing automatic nucleic acid extractor, which includes a rotary mixing component 1, a vertical moving component 2 that moves the rotary mixing component 1 downward to mix the sample in the deep well plate 6, a heating component 4 that is arranged at the lower part of the rotary mixing component 1 to heat the sample in the deep well plate 6 to meet the nucleic acid extraction conditions, a horizontal moving component 3 that moves the rotary mixing component 1 and the vertical moving component 2 horizontally to cooperate with the rotary mixing component 1, the vertical moving component 2 and the heating component 4 to complete the nucleic acid extraction, and a bottom plate 5 that supports the rotary mixing component 1, the vertical moving component 2, the horizontal moving component 3 and the heating component 4.

[0048] Rotating mixing component 1

[0049] like Figure 2 and Figure 3 As shown, the rotating mixing assembly 1 includes a magnetic rod assembly 11 and a magnetic rod sleeve rotating assembly 12 which are arranged up and down.

[0050] like Figure 2 As shown, the magnetic bar assembly 11 includes a magnetic bar support 111 and three rows of magnetic bars 112 arranged in parallel on the magnetic bar support 111, and eight magnetic bars 112 are arranged in each row at intervals.

[0051] like Figure 3 The structure diagram of the magnetic rod sleeve rotating assembly 12 is shown, which includes a first driving motor 121 with an output shaft arranged vertically downward, two primary gears 122: the first primary gear and the second and gear, four secondary gears 123: the first to fourth secondary gears, three rows of parallel tertiary gears 124: the first to third rows of tertiary gears, the center of each tertiary gear 124 in each row of tertiary gears is sleeved with a magnetic rod sleeve 125, and a magnetic rod sleeve support plate 126 supporting the above first driving motor 121 and multiple gears.

[0052] The output shaft of the first driving motor 121 is fixedly connected to the first primary gear 122, the first primary gear 122 is meshed with the first secondary gear and the second secondary gear, the second primary gear 122 is meshed with the third secondary gear and the fourth secondary gear; the first secondary gear is meshed with one of the three-stage gears 124 in the first row of three-stage gears 124, the second secondary gear and the third secondary gear are meshed with one of the three-stage gears 124 in the second row of three-stage gears 124, and the fourth secondary gear is meshed with one of the three-stage gears 124 in the third row of three-stage gears 124. Two adjacent three-stage gears 124 in the first to third rows of three-stage gears 124 are meshed. In order to make the structure of the magnetic rod sleeve rotating assembly 12 simple and orderly, the structure of the magnetic rod sleeve rotating assembly 12 is symmetrical, that is, each primary gear 122 is meshed with the second-stage gear 123 at the same position, and each second-stage gear 123 is meshed with the three-stage gear 124 at the same position in each row of three-stage gears 124.

[0053] When the first driving motor 121 is started, the first primary gear 122 connected thereto is driven to rotate, and then each of the three rows of three-stage gears 124 drives the magnetic rod sleeve 125 to rotate through the meshing of the primary gear 122, the secondary gear 123 and the tertiary gear 124.

[0054] Vertical moving component 2

[0055] like Figure 4 As shown, the vertical moving assembly 2 includes a magnetic rod sleeve moving assembly 21, a magnetic rod moving assembly 22, a slide rail assembly 23 for maintaining smooth movement of the magnetic rod sleeve moving assembly 21 and the magnetic rod moving assembly 22, and a vertical bracket 24 for supporting the magnetic rod sleeve moving assembly 21, the magnetic rod moving assembly 22 and the slide rail assembly 23.

[0056] The magnetic rod sleeve moving assembly 21 includes a second drive motor 211 and a first nut-screw mechanism connected to the second drive motor 211, wherein the first nut-screw mechanism includes a first screw 212 and a first nut 213 sleeved on the first screw 212, and the first nut 213 is fixedly connected to the magnetic rod sleeve support plate 126. When the second drive motor 211 is started, the first screw 212 is driven to rotate, and the first nut 213 sleeved on the first screw 212 moves up and down along the first screw 212, thereby driving the magnetic rod sleeve rotating assembly 12 to move up and down, so that the magnetic rod sleeve 125 enters and exits the deep hole plate 6.

[0057] The magnetic bar moving assembly 22 includes a third driving motor 221 and a second nut-screw mechanism connected to the third driving motor 221, wherein the second nut-screw mechanism includes a second screw 222 and a second nut 223 sleeved on the second screw 222, and the second nut 223 is fixedly connected to the magnetic bar bracket 111. When the third driving motor 221 is started, the second screw 222 is driven to rotate, and the second nut 223 sleeved on the second screw 222 moves up and down along the second screw 222, thereby driving the magnetic bar assembly 1 to move up and down, so that the magnetic bar 112 enters and exits the magnetic bar sleeve 125.

[0058] In some embodiments, in addition to using the first nut-screw mechanism and the second nut-screw mechanism to achieve linear movement in the above-mentioned magnetic rod sleeve moving assembly 21 and magnetic rod moving assembly 22, other linear drivers can be used to achieve linear movement, such as linear electric cylinders, linear electric rods, gear racks, etc. That is, the magnetic rod sleeve moving assembly 21 includes a first nut-screw mechanism as a first linear driver, and the magnetic rod moving assembly 22 includes a second nut-screw mechanism as a second linear driver.

[0059] The magnetic rod sleeve moving assembly 21 and the magnetic rod moving assembly 22 both move up and down smoothly along the slide rail assembly 23, wherein the slide rail assembly 23 includes a double slide rail 231 and a slider matched with the double slide rail 231. The magnetic rod sleeve moving assembly 21 and the magnetic rod moving assembly 22 both move up and down respectively along the double slide rail 231, so there are 4 sliders matched with the double slide rail 231, and the magnetic rod sleeve moving assembly 21 and the magnetic rod moving assembly 22 are respectively configured with 2 sliders 232.

[0060] Furthermore, in order to facilitate the connection between the magnetic rod sleeve moving assembly 21 and the magnetic rod sleeve rotating assembly 12, the magnetic rod sleeve moving assembly 21 also includes a first connecting plate 214 connected to the two sliders 232, and the first connecting plate 214 is mechanically connected to the magnetic rod sleeve supporting plate 126.

[0061] In order to facilitate the connection between the magnetic bar moving assembly 22 and the magnetic bar assembly 11, the magnetic bar moving assembly 22 further includes a second connecting plate 224 connected to the two sliders 232, and the second connecting plate 224 is mechanically connected to the magnetic bar bracket 111.

[0062] The magnetic rod sleeves 125 and magnetic rods 112 are arranged in three rows, each row is provided with 8 holes, and the deep well plate 6 is arranged in 6 rows horizontally, and each row is provided with 8 holes vertically, which correspond to the number of magnetic rod sleeves 125 and magnetic rods 112 in each row. The first row of holes of the deep well plate 6 is pre-placed with nucleic acid extraction samples, lysate and magnetic beads, and the 2nd to 5th rows are provided with a row of empty holes for placing the magnetic rod sleeves 125, and the other rows of holes are placed with cleaning solutions for washing and removing impurities, and the last row of holes is placed with elution solution to elute the nucleic acid from the magnetic beads.

[0063] When nucleic acid extraction is performed, the magnetic rod sleeve rotating assembly 12 with the three rows of magnetic rod sleeves 125 is driven by the magnetic rod sleeve moving assembly 21 to move downward into the first row of holes of the deep well plate 6, and then the magnetic rod assembly 11 with the three rows of magnetic rods 112 is driven by the magnetic rod moving assembly 22 to move downward into the corresponding magnetic rod sleeves 125. Since magnetic beads, nucleic acid extraction samples and lysis solutions are pre-placed in the first row of holes of the deep well plate 6, the nucleic acid molecules lysed from the nucleic acid extraction samples by the lysis solution are specifically adsorbed by the magnetic beads, and after the magnetic rods are extended into the magnetic rod sleeves 125 of the first row of the deep well plate 6, the magnetic beads are magnetically attracted to the magnetic rod sleeves 125 by the magnetic rods 112. In order to evenly mix the lysis solution and the nucleic acid extraction sample, each magnetic rod sleeve 125 rotates with the vertical axis as the rotation axis under the drive of the magnetic rod sleeve rotating assembly 12, and the magnetic rod is inserted into the first row of holes of the deep well plate 6 to mix the nucleic acid extraction sample and the lysis solution evenly, so that the nucleic acid molecules in the nucleic acid extraction sample are completely lysed. This rotation mixing method has a good mixing effect, thereby a good lysis effect, and no noise and bubbles are generated.

[0064] After the nucleic acid molecules in the first row of holes in the deep-well plate 6 are completely lysed and adsorbed on the magnetic beads, the nucleic acid molecules adsorbed on the magnetic beads need to be transferred to other holes in the deep-well plate 6 for washing, such as any three rows of holes from 2 to 5. At this time, the horizontal moving component 3 needs to drive the rotating mixing component 1 and the vertical moving component 2 to carry the magnetic rod 112 and the magnetic rod sleeve 125 to adsorb the magnetic beads and transfer them to any three rows of holes from 2 to 5 for washing and impurity removal.

[0065] After the nucleic acid molecules are cleaned and purified, the magnetic rod 112 and the magnetic rod cover 125 are driven by the horizontal moving component 3 to transfer the magnetic beads together to the last row of holes in the second to fifth holes for elution.

[0066] If the nucleic acid extraction efficiency is to be improved on the above-mentioned rotating mixing component 1, more rows of magnetic bars 112 and magnetic bar sleeves 125 can be provided. For the magnetic bar sleeve mixing component 12, this can be achieved by adding a primary gear, two secondary gears and a row of tertiary gears.

[0067] If only two rows of magnetic rods 112 and magnetic rod sleeves 125 are desired on the above-mentioned rotating mixing assembly 1, this can also be achieved by reducing the second primary gear, the third secondary gear and the fourth secondary gear.

[0068] Horizontal movement component 3

[0069] like Figure 5 As shown, the horizontal moving assembly 3 includes a fourth driving motor 31 with a horizontally arranged output shaft mounted on the base plate 5, an active synchronous wheel 32 coaxially connected to the output shaft of the fourth driving motor 31, a driven synchronous wheel 33, and a synchronous belt 34 sleeved on the active synchronous wheel 32 and the driven synchronous wheel 33, wherein the synchronous belt 34 is fixedly connected to the vertical bracket 24.

[0070] When the nucleic acid adsorbed on the magnetic beads needs to be washed and cleaned, the fourth drive motor 31 is controlled to start rotating, and drives the rotary mixing component 1 and the vertical moving component 2 to move horizontally for a distance through the master and slave synchronous pulleys 31, 32 and the synchronous belt 34.

[0071] In some embodiments, a driving sprocket may be used instead of the driving synchronous wheel 32 , a driven sprocket may be used instead of the driven synchronous wheel 33 , and a chain may be used instead of the synchronous belt 34 .

[0072] Heating element 4

[0073] During the lysis and elution, the liquids in the first and last rows of wells of the deep well plate 6 need to be heated to ensure successful lysis and elution.

[0074] like Figure 1 Each deep-well plate 6 shown includes 6 rows of deep wells, and each row includes 8 deep wells. That is, nucleic acid can be extracted through 8 channels at the same time for each deep-well plate 6. Three rows of magnetic rods 112 and three rows of magnetic rod sleeves 125 are arranged on the rotating mixing component 1, so the nucleic acid extractor of the utility model can extract nucleic acid from three deep-well plates 6 through 8 channels at the same time.

[0075] However, for each of the deep-hole plates 6 above, due to the needs of the calculation and extraction program, it is necessary to heat the liquid in the first row of deep holes and the last row of deep holes: the sixth row of deep holes. Therefore, a heating rod 41 is respectively arranged at the positions corresponding to the first row of deep holes and the last row of deep holes under each deep-hole plate 6. The heating rod 41 is arranged on a heating support plate 43. A temperature control plate 42 is arranged at the lower part of the heating support plate 43 to control the temperature of the heating rod 41.

[0076] In some embodiments, heating stickers are used instead of heating rods and are attached to the bottom of the first row of holes and the last row of holes in the deep well plate 6 .

[0077] Compared with the prior art, the utility model has the following beneficial effects:

[0078] The utility model rotary mixing automatic nucleic acid extractor rotates the magnetic rod sleeve 125 extending from the first row of holes to the last row of holes of the deep-well plate with a vertical axis as the rotation axis, so that the lysis solution and the nucleic acid extraction sample in the first row of holes of the deep-well plate are evenly mixed, the lysis rate is increased, and the nucleic acid extraction rate is high; the rotation in the last row of holes of the deep-well plate allows the elution solution to fully contact with the magnetic beads, and the nucleic acid molecules on the magnetic beads are completely eluted, thereby improving the elution rate; the rotation in the middle hole of the deep-well plate allows the cleaning solution to fully contact with the magnetic beads, so that the nucleic acid molecules on the magnetic beads are cleaned more cleanly.

[0079] In addition, the utility model rotary mixing automatic nucleic acid extractor adopts 3 rows of magnetic rods and 3 rows of magnetic rod covers, each row is provided with 8 magnetic rods and magnetic rod covers, forming a total of 24 channels for simultaneous nucleic acid extraction, which greatly improves the efficiency of nucleic acid extraction.

[0080] This specification uses embodiments to disclose the present application, including the best embodiment, and also enables those skilled in the art to practice the present application, including making and using any device or system and performing any incorporated method. The embodiments of the present application and the technical solutions obtained by slightly changing the embodiments are all within the scope of protection of this patent.

Claims

1. A rotary mixing automatic nucleic acid extractor, characterized in that: It includes: A vertical moving assembly (2) comprises a magnetic rod sleeve moving assembly (21), a magnetic rod moving assembly (22) and a vertical support (24) supporting the two; The rotating mixing component (1) comprises a magnetic rod component (11) having a magnetic rod (112) and a magnetic rod sleeve rotating component (12) having a magnetic rod sleeve (125) arranged above and below; the magnetic rod sleeve moving component (21) is connected to the magnetic rod sleeve rotating component (12) and drives the magnetic rod sleeve (125) to move downward and extend into a deep-well plate (6); the magnetic rod moving component (22) is connected to the magnetic rod component (11) and drives the magnetic rod (112) to move downward and extend into the magnetic rod sleeve (125) to adsorb the magnetic beads in the deep-well plate (6) onto the magnetic rod sleeve (125); the magnetic rod sleeve rotating component (12) drives the magnetic rod sleeve (125) to rotate and mix the nucleic acid extraction sample and other reagents in the deep-well plate (6); A heating component (4) is arranged at the bottom of the deep well plate (6) and is used to heat the nucleic acid extraction sample in the deep well plate (6) during the nucleic acid extraction process to meet the nucleic acid extraction conditions; A horizontal moving component (3) drives the vertical moving component (2) and the rotating mixing component (1) to move horizontally to complete all steps of nucleic acid extraction; A bottom plate (5) supports the rotating mixing component (1), the vertical moving component (2), the horizontal moving component (3) and the heating component (4).

2. The rotary mixing automatic nucleic acid extractor according to claim 1, characterized in that: The magnetic rod sleeve rotating assembly (12) comprises: A first driving motor (121), the output shaft of which is arranged vertically downward; A first primary gear (122) connected to an output shaft of the first drive motor (121); The first secondary gear and the second secondary gear are both meshed with the first primary gear (122); A first row of three-stage gears and a second row of three-stage gears are arranged in parallel, the first secondary gear is meshed with one of the three-stage gears (124) in the first row of three-stage gears, the second secondary gear is meshed with one of the three-stage gears (124) in the second row of three-stage gears, and two adjacent three-stage gears (124) in the first row of three-stage gears and the second row of three-stage gears are meshed, and the center of each of the three-stage gears (124) is sleeved with the magnetic rod sleeve (125); A magnetic rod sleeve support plate (126) supports the first drive motor (121) and the magnetic rod sleeve (125), and the magnetic rod sleeve support plate (126) is connected to the magnetic rod sleeve moving component (21).

3. The rotary mixing automatic nucleic acid extractor according to claim 2, characterized in that: The magnetic rod sleeve rotating assembly (12) further comprises: a second primary gear, a third secondary gear and a fourth secondary gear, wherein the second primary gear is meshed with the third secondary gear and the fourth secondary gear, and the third secondary gear is meshed with one of the third-stage gears (124) in the second row of third-stage gears; The third row of three-stage gears is parallel to the second row of three-stage gears, and one of the three-stage gears (124) in the third row of three-stage gears is meshed with the fourth two-stage gear; two adjacent three-stage gears (124) in the third row of three-stage gears are meshed, and the center of each of the three-stage gears (124) in the third row of three-stage gears is sleeved with the magnetic rod sleeve (125).

4. The rotary mixing automatic nucleic acid extractor according to claim 3, characterized in that: The first, second and third rows of three-stage gears are each provided with eight three-stage gears (124).

5. The rotary mixing automatic nucleic acid extractor according to any one of claims 2 to 4, characterized in that: The magnetic rod sleeve moving assembly (21) comprises a second drive motor (211) and a first linear drive connected to the second drive motor (211), wherein the first linear drive is connected to the magnetic rod sleeve supporting plate (126).

6. The rotary mixing automatic nucleic acid extractor according to claim 5, characterized in that: The first linear drive is a first nut-screw mechanism, which comprises a first screw (212) connected to the second drive motor (211) and a first nut (213) sleeved on the first screw (212), wherein the first nut (213) is fixedly connected to the magnetic rod sleeve support plate (126).

7. The rotary mixing automatic nucleic acid extractor according to claim 5 or 6, characterized in that: The magnetic rod moving assembly (22) comprises a third driving motor (221) and a second linear driver connected to the third driving motor (221); The magnetic bar assembly (11) comprises a magnetic bar support (111) and a plurality of magnetic bars (112) arranged in parallel on the magnetic bar support (111); The second linear drive is connected to the magnetic rod support (111).

8. The rotary mixing automatic nucleic acid extractor according to claim 7, characterized in that: The second linear drive is a second nut-screw mechanism, the second nut-screw mechanism comprising a second screw (222) and a second nut (223) sleeved on the second screw (222), the second nut (223) being fixedly connected to the magnetic rod support (111).

9. The rotary mixing automatic nucleic acid extractor according to claim 7, characterized in that: The vertical moving assembly (2) further comprises a slide rail assembly (23), which comprises a double slide rail (231) and four sliding blocks (232) matched with the double slide rail (231); The magnetic rod sleeve moving assembly (21) further comprises a first connecting plate (214) fixedly connected to the two sliding blocks (232), and the first connecting plate (214) is fixedly connected to the magnetic rod sleeve supporting plate (126); The magnetic bar moving assembly (22) further comprises a second connecting plate (224) fixedly connected to the two sliding blocks (232), and the second connecting plate (224) is fixedly connected to the magnetic bar bracket (111).

10. The rotary mixing automatic nucleic acid extractor according to claim 1, characterized in that: The horizontal moving assembly (3) comprises a fourth driving motor (31) with an output shaft horizontally arranged and mounted on the base plate (5), an active synchronous wheel (32) coaxially connected to the output shaft of the fourth driving motor (31), a driven synchronous wheel (33), and a synchronous belt (34) sleeved on the active synchronous wheel (32) and the driven synchronous wheel (33), wherein the synchronous belt (34) is fixedly connected to the vertical bracket (24).

11. The rotary mixing automatic nucleic acid extractor according to claim 1, characterized in that: The horizontal moving assembly (3) comprises a fourth driving motor (31) with an output shaft horizontally arranged and mounted on the base plate (5), a driving sprocket and a driven sprocket coaxially connected to the output shaft of the fourth driving motor (31), and a chain sleeved on the driving sprocket and the driven sprocket, wherein the chain is fixedly connected to the vertical bracket (24).

12. The rotary mixing automatic nucleic acid extractor according to claim 1, characterized in that: The heating assembly (4) comprises a heating rod (41) arranged at the bottom of the deep well plate (6), a heating support plate (43) supporting the heating rod (41), and a temperature control plate (42) arranged below the heating support plate (43) for controlling the temperature of the heating rod (41).

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