Fluorescence detection device and sample detection equipment

Abstract

The utility model provides a fluorescence detection device and sample detection equipment, the fluorescence detection device comprises a consumable seat, an excitation light path and an emission light path, the consumable seat is provided with a sample hole and two light guide grooves, and the sample hole of a focus lens is used for accommodating a reaction container; an excitation light source, an excitation collimating lens, an excitation light filter and an excitation focusing lens are sequentially arranged on the excitation light path; an emission collimating mirror, an emission optical filter, an emission focusing mirror and a detector are sequentially arranged on the emission optical path, and passbands of the excitation optical filter and the emission optical filter are not overlapped. The excitation light path and the emission light path only occupy the bottom surface and one side surface of the consumable seat, and other side surfaces are reserved for mounting the temperature control device, so that the fluorescence detection device is small, compact and practical in structure, the cost of the fluorescence detection device can be reduced, and the reliability and the sensitivity are higher.

Description

Technical Field

[0001] The utility model belongs to the technical field of sample detection, and in particular relates to a fluorescence detection device and a sample detection equipment. Background Art

[0002] PCR technology is a molecular biology technology that amplifies specific DNA fragments in vitro. Its biggest feature is that it can greatly increase the amount of DNA. PCR technology has the characteristics of strong specificity, high sensitivity, low purity requirements, simplicity and rapidity, and is therefore widely used in molecular biology detection and analysis.

[0003] At present, the mainstream PCR instrument fluorescence detection devices on the market mostly use turntable scanning and optical fiber light guidance; this type of fluorescence detection device is large in size, and the optical fiber assembly is inconvenient and expensive. Other devices use camera-based fluorescence detection methods, which have high optical path costs and large volumes, and the consumables need to be sealed before use. Both optical paths are not suitable for portable POCT application scenarios.

[0004] Some portable devices on the market use a coaxial optical path configuration, where the fluorescence excitation light path and the fluorescence emission light path are coaxially arranged in a mechanical housing, and a dichroic mirror is used to separate the laser light and the emission light. In this configuration, the fluorescence emission light is easily affected by the excitation light, and in practice, the fluorescence background is high, resulting in low system sensitivity. Utility Model Content

[0005] The main purpose of the utility model is to provide a fluorescence detection device and a sample detection equipment, aiming to solve the technical problems that the fluorescence detection device of the sample detection equipment in the prior art is large in size and high in cost.

[0006] In order to achieve the above-mentioned purpose, the utility model provides a fluorescence detection device, which includes: a consumables holder, which is provided with a sample hole and two light guide grooves, the sample hole is used to accommodate a reaction container, one of the light guide grooves is arranged at the bottom of the sample hole, and the other light guide groove is arranged at the side of the sample hole; an excitation light path is arranged corresponding to one of the light guide grooves, and an excitation light source, an excitation collimator, an excitation filter and an excitation focusing mirror are arranged on the excitation light path in sequence, and the colors of light emitted by the multiple excitation light sources are all different; an emission light path is arranged corresponding to the other light guide groove, and an emission collimator, an emission filter, an emission focusing mirror and a detector are arranged on the emission light path in sequence, wherein the passbands of the excitation filter and the emission filter have no overlapping areas.

[0007] In an embodiment of the utility model, the excitation collimator is a multi-element matrix lens, and the number of the excitation collimators and the number of the excitation light sources are the same and are arranged in a one-to-one correspondence.

[0008] In an embodiment of the utility model, the excitation filter is a single-bandpass filter, a plurality of the excitation filters are distributed in a matrix, and the number of the excitation light sources and the excitation filters are consistent and are arranged in a one-to-one correspondence.

[0009] In an embodiment of the utility model, the excitation filter is a multi-bandpass filter, and the passbands of the excitation filter correspond one-to-one to the multiple excitation light sources.

[0010] In an embodiment of the present invention, the passbands of the excitation filter and the emission filter are 10 nm or more apart.

[0011] In an embodiment of the utility model, the emission filter is a single-bandpass filter, a plurality of the emission filters are distributed in a matrix, and the number of the emission filters and the number of the excitation light sources are consistent and are arranged in a one-to-one correspondence.

[0012] In an embodiment of the utility model, the emission focusing mirrors are multiple in number and distributed in a matrix, and the number of the emission focusing mirrors and the number of the excitation light sources are consistent and are arranged in a one-to-one correspondence.

[0013] In an embodiment of the utility model, the detector is a multi-segment photodiode; or, the detector is a single-point detector, and the number of the single-point detectors and the excitation light sources is consistent and arranged in a one-to-one correspondence.

[0014] In an embodiment of the present invention, the emission filter is a multi-bandpass filter.

[0015] The utility model also provides a sample detection device, which comprises a temperature control device and the fluorescence detection device as described above, wherein the temperature control device is used to adjust the sample temperature in the reaction container.

[0016] Through the above technical solution, the fluorescence detection device provided by the embodiment of the utility model has the following beneficial effects:

[0017] When using a fluorescence detection device for fluorescence detection, the reaction container can be placed in the sample hole, so that the light emitted by the excitation light source is focused by the excitation collimator, the excitation filter, and the excitation focusing lens, and then passes through the light guide groove and the transparent reaction container to enter the reaction liquid in the reaction container, thereby exciting the reaction liquid inside the reaction container to emit fluorescence. The emission light path is installed below the bottom of the reaction container and can receive the fluorescence emitted by the reaction container. The fluorescence can be transmitted to the detector through the emission collimator, the emission filter, and the emission focusing lens in sequence, and the detector can perform fluorescence detection. The excitation light path and emission light path in the utility model only occupy the bottom surface and one side surface of the consumables holder, and the other side surface is reserved for the installation of the temperature control device, so that the fluorescence detection device has a small, compact and practical structure; and there are no moving or dynamic parts in the light path, and the passbands of the excitation filter and the emission filter have no overlapping areas, so as to avoid the use of dichroic mirrors, reduce the cost of the fluorescence detection device and avoid the situation where the emission light is affected by the excitation light, and have high reliability and sensitivity. Another fluorescence detection device can perform fluorescence detection of multiple channels on multiple excitation light sources at the same time, support multiple PCR, facilitate expansion, and support the detection of more tubes.

[0018] Other features and advantages of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings are used to provide an understanding of the present invention and constitute a part of the specification. Together with the following specific embodiments, they are used to explain the present invention, but do not constitute a limitation to the present invention. In the accompanying drawings:

[0020] Figure 1 It is a structural schematic diagram of a fluorescence detection device according to an embodiment of the utility model;

[0021] Figure 2 is a structural schematic diagram of a fluorescence detection device according to another embodiment of the utility model;

[0022] Figure 3 is a structural schematic diagram of a fluorescence detection device according to another embodiment of the utility model;

[0023] Figure 4 is a structural schematic diagram of a fluorescence detection device according to yet another embodiment of the utility model;

[0024] Figure 5 It is a structural schematic diagram of a fluorescence detection device according to an embodiment of the utility model.

[0025] Description of Reference Numerals

[0026] Label Name Label Name

[0027] 100 Fluorescence detection device 23 Excitation filter

[0028] 1 Consumables holder 24 Excitation focusing lens

[0029] 11 Sample hole 3 Emission light path

[0030] 12 Light guide slot 31 Emitting collimator

[0031] 2 Excitation light path 32 Emission filter

[0032] 21 Excitation light source 33 Emission focusing lens

[0033] 22 Excitation collimator 34 Detector DETAILED DESCRIPTION

[0034] The specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0035] The fluorescence detection device according to the present invention is described below with reference to the accompanying drawings.

[0036] like Figures 1 to 5 As shown, the fluorescence detection device 100 includes a consumable holder 1, an excitation light path 2 and an emission light path 3. The consumable holder 1 is provided with a sample hole 11 and two light guide grooves 12. The sample hole 11 is used to accommodate a reaction container, wherein one of the light guide grooves 12 is arranged at the bottom of the sample hole 11, and the other light guide groove 12 is arranged at the side of the sample hole 11; the excitation light path 2 is corresponding to one of the light guide grooves 12 and is arranged at the end of the light guide groove 12 away from the sample hole 11. The excitation light path 2 is sequentially provided with an excitation light source 21, an excitation collimator 22, an excitation filter 23 and an excitation focusing lens 24. The light emitted by the multiple excitation light sources 21 can sequentially pass through the excitation light source 21, An excitation collimator 22, an excitation filter 23, an excitation focusing mirror 24 and a light guide groove 12 are provided to excite the sample in the reaction container. The light colors emitted by the multiple excitation light sources 21 are all different. An emission light path 3 corresponds to another light guide groove 12 and is arranged at an end of the light guide groove 12 away from the sample hole 11. An emission collimator 31, an emission filter 32, an emission focusing mirror 33 and a detector 34 are sequentially arranged on the emission light path 3. The fluorescence energy in the reaction container is sequentially transmitted to the detector 34 through the emission collimator 31, the emission filter 32 and the emission focusing mirror 33, wherein the passbands of the excitation filter 23 and the emission filter 32 have no overlapping areas.

[0037] like Figures 2 to 5As shown, the light guide groove 12 in this embodiment is conical, and the conical bottom surface of the light guide groove 12 is arranged away from the sample hole 11, the tip of the light guide groove 12 is arranged close to the sample hole 11, and the top of the sample hole 11 is open, and the reaction container can be placed into the sample hole 11 through the top. The central axes of the two light guide grooves 12 are arranged perpendicular to each other, so that the fluorescence detection device 100 in this embodiment has a compact structure. The excitation light source 21 can be an LED lamp, and an LED with a low divergence angle can be used. In one embodiment, the excitation light source 21 is an LED with a divergence angle less than 15 degrees. Figure 1 As shown, the number of the excitation light sources 21 in this embodiment is four, and the four excitation light sources 21 are distributed in a matrix. In other embodiments, the number of the excitation light sources 21 is set according to actual usage requirements.

[0038] When the fluorescence detection device 100 in this embodiment is used for fluorescence detection, the reaction container can be placed in the sample hole 11, so that the light emitted by the excitation light source 21 is focused by the excitation collimator 22, the excitation filter 23, and the excitation focusing lens 24, passes through the light guide groove 12, and penetrates the transparent reaction container to enter the reaction liquid in the reaction container, thereby exciting the reaction liquid inside the reaction container to emit fluorescence. The emission light path 3 is installed below the bottom of the reaction container and can receive the fluorescence emitted by the reaction container. The fluorescence can be transmitted to the detector 34 through the emission collimator 31, the emission filter 32, and the emission focusing lens 33 in sequence, and the detector 34 can perform fluorescence detection. The excitation light path 2 and the emission light path 3 in this embodiment only occupy the bottom surface and one side surface of the consumable holder 1, and the other side surfaces are reserved for the installation of the temperature control device, so that the fluorescence detection device 100 is small, compact and practical; and there are no moving or dynamic parts in the light path, and the passbands of the excitation filter 23 and the emission filter 32 have no overlapping areas, so as to avoid the use of dichroic mirrors, which can reduce the cost of the fluorescence detection device 100 and avoid the situation where the emission light is affected by the excitation light, and the reliability and sensitivity are high. In addition, the fluorescence detection device 100 can perform fluorescence detection of multiple channels on multiple excitation light sources 21 at the same time, support multiple PCR, facilitate expansion, and support the detection of more tubes.

[0039] Among them, the emission collimator 31 and the emission focusing lens 33 can be one of a spherical surface, a spherical lens, a Fresnel lens or an array lens. In one embodiment, the emission filter 32 can be four single-bandpass filters arranged in an array, and each filter corresponds to a detection channel; in another embodiment, the emission filter 32 can also be a single-piece four-bandpass filter, each bandpass corresponds to a detection channel, and the cutoff depth of the filter reaches OD5 or more, and the passbands of the excitation filter 23 and the emission filter 32 have no overlapping area, and are at least 10nm or more apart.

[0040] In one embodiment, the excitation collimator 22 is a multi-element matrix lens, and the number of the excitation collimator 22 and the excitation light source 21 are the same and are arranged in a one-to-one correspondence.

[0041] The excitation filter 23 and the emission filter 32 are configured as follows:

[0042] Excitation filter passband Emission filter channel FAM 460nm-485nm 500nm-515nm HEX 525nm-540nm 555nm-570nm ROX 580nm-595nm 605nm-620nm CY5 635nm-655nm 670nm-690nm

[0043] The excitation light source 21 corresponding to the FAM channel is turned on. At this time, after the excitation light passes through the FAM channel excitation filter 23, the light of the spectrum between 460nm-485nm is transmitted, and the light of other spectrums is cut off by the filter;

[0044] In the corresponding emission light path 3, the multi-bandpass emission filter 32 can transmit the fluorescence of the FAM channel, allowing the fluorescence of 500-515nm to be transmitted, while cutting off the excitation light of 460-485nm; the cut-off depth is OD5 and above;

[0045] The excitation light source 21 corresponding to the HEX channel is turned on. At this time, after the excitation light passes through the excitation filter 23 of the HEX channel, the light of the spectrum between 525nm-540nm is transmitted, and the light of other spectrums is cut off by the filter;

[0046] In the corresponding emission light path 3, the multi-bandpass emission filter 32 can transmit the fluorescence of the HEX channel, allowing the fluorescence between the 555-570nm spectrum to be transmitted, and other spectra are cut off; the cut-off depth is OD5 and above;

[0047] The excitation light source 21 corresponding to the ROX channel is turned on. At this time, after the excitation light passes through the excitation filter 23 of the ROX channel, the light of the spectrum between 580nm-595nm is transmitted, and the light of other spectrums is cut off by the filter;

[0048] In the corresponding emission light path 3, the multi-bandpass emission filter 32 can transmit the fluorescence of the ROX channel, allowing the fluorescence between 605-620nm spectrum to be transmitted, and other spectrums are cut off; the cut-off depth is OD5 and above;

[0049] The excitation light source 21 corresponding to the CY5 channel is turned on. At this time, after the excitation light passes through the excitation filter 23 of the CY5 channel, the light of the spectrum between 635nm-655nm is transmitted, and the light of other spectrums is cut off by the filter;

[0050] In the corresponding emission light path 3, the multi-bandpass emission filter 32 can transmit the fluorescence of the CY5 channel, allowing the fluorescence between the 670-690nm spectrum to transmit, and cutting off other spectra; the cutoff depth is OD5 and above.

[0051] The detection channels in this embodiment include a FAM channel, a HEX channel, a ROX channel and a CY5 channel, and the four detection channels correspond to four excitation light sources 21. The excitation filter 23 is a single-bandpass filter, and the emission filter 32 is a multi-bandpass filter.

[0052] In one embodiment, the excitation filter 23 is a single bandpass filter, and a plurality of excitation filters 23 are arranged in a matrix, and the number of excitation light sources 21 and the number of excitation filters 23 are the same and arranged in a one-to-one correspondence. The use of a single bandpass filter has a lower cost, and the matrix-distributed excitation filters 23 can improve the compactness of the structure of the excitation light path 2, making the fluorescence detection device 100 small in size while reducing the production cost.

[0053] In another embodiment, the excitation filter 23 is a multi-bandpass filter, and the passbands of the excitation filter 23 correspond one-to-one to the multiple excitation light sources 21. In this embodiment, the excitation filter 23 is a single piece, and multiple excitation light sources 21 share the same excitation filter 23, which reduces the filter components and facilitates the assembly of the excitation light path 2.

[0054] Specifically, the passbands of the excitation filter 23 and the emission filter 32 are 10 nm or more apart. In this embodiment, the passbands of the excitation filters 23 and the emission filters 32 differ by at least 10 nm. Through the passband setting in this embodiment, the compactness of the structure between the excitation light path 2 and the emission light path 3 can be further improved. In the case of a small spacing, the emission light can be prevented from being affected by the excitation light, thereby ensuring the detection reliability of the fluorescence detection device 100.

[0055] In one embodiment, the emission filter 32 is a single-bandpass filter, and a plurality of emission filters 32 are arranged in a matrix, and the number of emission filters 32 and the number of excitation light sources 21 are the same and are arranged one by one. Figure 3 As shown, in this embodiment, four emission filters 32 are arranged in a matrix, and in other embodiments, the number of emission filters 32 can be set according to actual use requirements. Single-bandpass filters are relatively low in price, which can reduce the production cost of the fluorescence detection device 100.

[0056] Specifically, the number of emission focusing mirrors 33 is multiple and arranged in a matrix, and the number of emission focusing mirrors 33 and the number of excitation light sources 21 are consistent and arranged one by one. In one embodiment, the detector 34 is a multi-segment photodiode, and in another embodiment, the detector 34 is a single-point detector distributed in a matrix, and the number of single-point detectors and the excitation light sources 21 are consistent and arranged one by one. The detector 34 can be one of a photodiode single-point detector, a four-quadrant detector, a segmented photodiode detector, a CMOS, a CCD imaging device, or a spectral detection device.

[0057] In one embodiment, the emission filter is a multi-bandpass filter. In this embodiment, multiple excitation light sources 21 share one emission filter 32, which reduces the number of filter components and facilitates the assembly of the fluorescence detection device 100.

[0058] like Figure 1 As shown, in the first embodiment, the excitation light path 2 is located on the right side of the sample hole 11 in the horizontal direction, and the emission light path 3 is located below the sample hole 11 in the vertical direction. Four excitation light sources 21 correspond to four 2*2 array excitation collimators 22, four 2*2 array excitation filters 23 and an excitation focusing mirror 24, and one emission collimator 31 corresponds to four 2*2 array emission filters 32, an emission focusing mirror 33 and a single PD detector, which can perform four-channel fluorescence detection.

[0059] like Figure 2 As shown, in the second embodiment, four 2*2 array excitation light sources 21 correspond to four 2*2 array excitation collimators 22, four 2*2 array excitation filters 23 and an excitation focusing lens 24, and one emission collimator 31 corresponds to one emission filter 32, one emission focusing lens 33 and a single detector 34, so that four-channel fluorescence detection can be performed.

[0060] like Figure 3 As shown, in the third embodiment, four 2*2 array excitation light sources 21 correspond to four 2*2 array excitation collimators 22, four 2*2 array excitation filters 23 and an excitation focusing lens 24, and one emission collimator 31 corresponds to four fan-shaped and circularly distributed emission filters 32, four 2*2 array emission focusing lenses 33 and four 2*2 array detectors 34, which can perform four-channel fluorescence detection.

[0061] like Figure 4 As shown, in the fourth embodiment, four 2*2 array excitation light sources 21 correspond to four 2*2 array excitation collimators 22, four 2*2 array excitation filters 23 and an excitation focusing lens 24, and an emission collimator 31 corresponds to an emission filter 32, four 2*2 array emission focusing lenses 33 and a four-segment photodiode, which can perform four-channel fluorescence detection. The four-segment photodiode integrates four single-point detectors; each segment represents a single-point detector and outputs an independent signal; the intensities of the four output signals respectively represent the fluorescence intensities of the fluorescent probes of the four targets to be detected, such as the fluorescent probes FAM, HEX, ROX and CY5;

[0062] In one embodiment, the detector 34 may also be an array of four independent detectors. The four independent single-point detectors have greater flexibility in the configuration of fluorescent probes and are suitable for the detection of some special fluorescent probes, such as FRET fluorescent probes, and even the detection of long Stokes shift fluorescent probes.

[0063] The utility model also proposes a sample detection device, which includes a temperature control device and the above fluorescence detection device 100, and the temperature control device is used to adjust the sample temperature in the reaction container. In one embodiment, the temperature control device can adopt the heating structure in the prior art, and the sample detection device includes a plurality of fluorescence detection devices 100, each of which can perform multi-channel fluorescence detection, and the consumable holders 1 are arranged side by side, the light guide groove 12 at the bottom corresponds to the emission light path 3, and the light guide groove 12 at the side corresponds to the excitation light path 2, and the sides of two adjacent consumable holders 1 are connected. The sample detection device can perform multi-channel fluorescence detection on samples in multiple reaction containers at the same time, further improving the detection efficiency, and by arranging the consumable holders 1 in a side-by-side manner, the compactness of the structure of the sample detection device can be improved, and the miniaturization of the sample detection device can be achieved. In one embodiment, the sample detection device can be a nucleic acid amplification device.

[0064] like Figure 5 As shown, in the fifth embodiment, four excitation light sources 21, four array excitation collimators 22, four excitation filters 23 and an excitation focusing mirror 24 are arranged in sequence on the excitation light path, and an emission collimator 31, four emission filters 32, four emission focusing mirrors 33 and a detector 34 are arranged in sequence on the emission light path.

[0065] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0066] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0067] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0068] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A fluorescence detection device (100), characterized in that: The fluorescence detection device (100) comprises: A consumables holder (1) is provided with a sample hole (11) and two light guide grooves (12), wherein the sample hole (11) is used to accommodate a reaction container, wherein one light guide groove (12) is arranged at the bottom of the sample hole (11), and the other light guide groove (12) is arranged at the side of the sample hole (11); An excitation light path (2) is provided corresponding to one of the light guide slots (12), and an excitation light source (21), an excitation collimator (22), an excitation filter (23) and an excitation focusing lens (24) are sequentially provided on the excitation light path (2), and the colors of light emitted by the multiple excitation light sources (21) are all different; An emission light path (3) is arranged corresponding to another of the light guide slots (12), and an emission collimator (31), an emission filter (32), an emission focusing lens (33) and a detector (34) are arranged in sequence on the emission light path (3), wherein the passbands of the excitation filter (23) and the emission filter (32) have no overlapping regions.

2. The fluorescence detection device according to claim 1, characterized in that: The excitation collimator (22) is a multi-element matrix lens, and the number of the excitation collimator (22) and the excitation light source (21) are the same and are arranged in a one-to-one correspondence.

3. The fluorescence detection device according to claim 1, characterized in that: The excitation filter (23) is a single-bandpass filter, a plurality of the excitation filters (23) are distributed in a matrix, and the number of the excitation light sources (21) and the excitation filters (23) is the same and they are arranged in a one-to-one correspondence.

4. The fluorescence detection device according to claim 1, characterized in that: The excitation filter (23) is a multi-bandpass filter, and the passbands of the excitation filter (23) correspond one-to-one to the plurality of excitation light sources (21).

5. The fluorescence detection device according to any one of claims 1 to 4, characterized in that: The passbands of the excitation filter (23) and the emission filter (32) are separated by 10 nm or more.

6. The fluorescence detection device according to any one of claims 1 to 4, characterized in that: The emission filter (32) is a single-bandpass filter, a plurality of the emission filters (32) are distributed in a matrix, and the number of the emission filters (32) and the number of the excitation light sources (21) are consistent and are arranged in a one-to-one correspondence.

7. The fluorescence detection device according to any one of claims 1 to 4, characterized in that: The emission focusing mirrors (33) are multiple in number and distributed in a matrix, and the emission focusing mirrors (33) and the excitation light sources (21) are consistent in number and are arranged in a one-to-one correspondence.

8. The fluorescence detection device according to any one of claims 1 to 4, characterized in that: The detector (34) is a multi-segment photodiode; or, The detector (34) is a single-point detector, and the number of the single-point detectors and the number of the excitation light sources (21) are the same and are arranged in a one-to-one correspondence.

9. The fluorescence detection device according to any one of claims 1 to 4, characterized in that: The emission filter (32) is a multi-bandpass filter.

10. A sample detection device, characterized in that: The sample detection device comprises a temperature control device and the fluorescence detection device (100) according to any one of claims 1 to 9, wherein the temperature control device is used to adjust the sample temperature in the reaction container.

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