Experimental device for batch research of plant mycorrhiza infection rate

Abstract

The utility model relates to an experimental device for researching the infection rate of plant mycorrhiza in batches. The experimental device comprises an experimental box and an experimental assembly, the experiment box is opened, the experiment assembly comprises a reagent groove, a sample basket and a reagent bottle, a valve is arranged on the reagent groove, the valve is connected with the reagent bottle, and the sample basket is arranged in the reagent groove. The number of the experiment assemblies is multiple, and the experiment assemblies are arranged in the experiment box. According to the experimental device, the corn root systems can be treated in batches, before an experiment, an operator can inject reagents needed to be used into all the reagent bottles according to experiment requirements, in the experiment process, experiment samples are placed on the sample baskets, the samples are sequentially placed in the experiment assemblies of the corresponding reagents according to an experiment method, and then the samples are placed in the experiment assemblies of the corresponding reagents; the utility model provides an experimental device for researching the infection rate of plant mycorrhiza in batches, which can treat samples in batches, and is convenient to operate and higher in experimental efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of plant experiments, in particular to an experimental device for batch research of plant mycorrhizal infection rates. Background Art

[0002] The observation of mycorrhizal growth and the determination of infection rates play an important role in mycorrhizal research. This process involves the treatment of plant roots, which usually requires multiple pouring and cleaning of reagents. This is not only time-consuming and labor-intensive, but also has some technical challenges.

[0003] When conducting batch mycorrhizal experiments, it is often necessary to frequently soak, pour, and replace reagents, which results in a long experiment time and poor experimental efficiency. Summary of the invention

[0004] In view of the above technical problems, the utility model provides an experimental device for batch research of plant mycorrhizal infection rate, which can process mycorrhizae in batches, is convenient to operate, and has high experimental efficiency.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] An experimental device for batch research of plant mycorrhizal infection rate, comprising:

[0007] An experimental box, wherein the experimental box is open; and

[0008] An experimental component, the experimental component comprising a reagent tank, a sample basket and a reagent bottle, the reagent tank is provided with a valve, the valve is connected to the reagent bottle, and the sample basket is arranged in the reagent tank;

[0009] The experimental components are provided in a plurality and are arranged in the experimental box;

[0010] A plurality of slots are separately arranged in the sample basket;

[0011] The experimental components are arranged side by side inside the experimental box;

[0012] The experimental box is also provided with a water tank, which is arranged along the arrangement direction of the experimental components and correspondingly arranged on the side of the reagent tank;

[0013] The water tank is provided with a water outlet valve, and the experimental box is also provided with a clean water bottle, which supplies clean water to the water outlet valve.

[0014] In one possible implementation manner, the experimental component further includes a drain valve, which is disposed outside the reagent tank to discharge the liquid inside the reagent tank.

[0015] In one possible implementation manner, a scale is also provided on the inner wall of the reagent tank.

[0016] In one possible implementation manner, the experimental component further includes an electric heating plate, which is disposed at the bottom of the reagent tank to heat the liquid inside the reagent tank.

[0017] In one possible implementation manner, a plurality of protrusions are provided at the bottom of the reagent tank, and the protrusions are used to bear the weight and support the sample basket.

[0018] The utility model adopts the above technical solution, which has the following advantages:

[0019] The experimental device can process mycorrhizae in batches. Before the experiment, the operator can inject the required reagents into each reagent bottle according to the experimental needs. During the experiment, the experimental mycorrhizae are placed on the sample basket. According to the experimental method determined in advance, the mycorrhizae are placed in the experimental components of the corresponding reagents in sequence. The utility model provides an experimental device for batch research of plant mycorrhizae infection rate, which can process mycorrhizae in batches, is convenient to operate, and has high experimental efficiency.

[0020] Efficient batch processing: The device allows multiple samples to be processed simultaneously, significantly improving experimental efficiency. Operators can inject the required reagents at one time according to experimental requirements, meeting the overall needs of the experiment;

[0021] Easy to operate: The design of the reagent bottle and valve simplifies the liquid injection process, making it easier for operators to quickly control the experimental steps, reducing experimental time and labor costs;

[0022] Flexibility: The design of the sample basket allows multiple troughs to be separated in the same sample basket, enabling simultaneous immersion of multiple mycorrhizae, increasing the scope of application and flexibility of the device;

[0023] Optimized liquid flowability: The raised design at the bottom of the reagent tank supports the sample basket, preventing the bottom of the sample basket from directly contacting the bottom of the tank, improving liquid flowability and achieving a more uniform immersion effect;

[0024] Convenient drain function: The external drain valve allows operators to quickly drain the liquid in the reagent tank, further enhancing the flexibility and convenience of use;

[0025] Intuitive observation design: The inner wall of the reagent tank is set with scales, which is convenient for operators to observe the liquid volume in real time and ensure the accuracy during the experiment;

[0026] Heating function: The electric heating plate configuration at the bottom enables the operator to heat the liquid according to the experimental requirements, optimize the immersion conditions of the liquid, and improve the practicality and reliability of the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the specific structure of an experimental device in one embodiment of the utility model;

[0028] Figure 2 This is a schematic diagram of the specific structure of an experimental device in another embodiment of the utility model;

[0029] Reference numerals:

[0030] 1. Experiment box; 2. Experimental components; 21. Reagent tank; 22. Sample basket; 221. Tank body; 23. Reagent bottle; 24. Valve; 25. Drain valve; 3. Water tank; 31. Water outlet valve. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model is described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, all other embodiments obtained by ordinary persons in the field without creative work are within the scope of protection of the utility model.

[0032] Unless otherwise defined, the technical or scientific terms used in the present invention shall have the usual meanings understood by persons with ordinary skills in the field to which the present invention belongs. The words "first", "second", "third", "fourth" and similar terms used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Words such as "include" or "comprise" and the like mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. Words such as "connect" or "connected" and the like are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

[0033] The observation of mycorrhizal growth and the determination of infection rate occupy an important position in mycorrhizal research. This process involves the treatment of plant roots, and usually requires multiple pouring and cleaning of reagents. This is not only time-consuming and labor-intensive, but also involves some technical challenges. When conducting batch mycorrhizal experiments, it is often necessary to frequently soak, pour, and replace reagents, resulting in a long experiment time and poor experimental efficiency. In view of the above-mentioned technical problems, the utility model provides an experimental device for batch research on the infection rate of plant mycorrhizae, which can process mycorrhizae in batches, is easy to operate, and has high experimental efficiency. The technical scheme of the utility model is described in detail below in conjunction with specific examples.

[0034] Reference Figure 1As shown, the experimental device for batch research of plant mycorrhizal infection rate involved in the utility model comprises an experimental box 1 and an experimental component 2. The experimental box 1 is open, and the experimental component 2 comprises a reagent tank 21, a sample basket 22 and a reagent bottle 23. The reagent tank 21 is provided with a valve 24, the valve 24 is connected to the reagent bottle 23, and the sample basket 22 is arranged in the reagent tank 21. Among them, there are several experimental components 2, which are arranged in the experimental box 1.

[0035] Specifically, in this embodiment, four experimental components 2 are provided, and the experimental components 2 are arranged side by side inside the experimental box 1 .

[0036] It should be noted that, for the convenience of operation, the reagent bottle 23 can be connected to the valve 24 via a water pump, and when liquid needs to be injected, the valve 24 can be directly operated.

[0037] Illustratively, the experimental device can process the root system in batches. Before the experiment, the operator can inject the required reagents into each reagent bottle 23 according to the experimental needs. During the experiment, the experimental samples are placed on the sample basket 22. According to the pre-determined experimental method, the samples are placed in the experimental components 2 of the corresponding reagents in sequence. The utility model provides an experimental device for batch research on the infection rate of plant mycorrhizae, which can process mycorrhizae in batches, is easy to operate, and has high experimental efficiency.

[0038] In one embodiment, the overall structure of the sample basket 22 is further refined, and a plurality of slots 221 are separately arranged in the sample basket 22. According to experimental requirements, during the design and manufacturing stage of the device, sample baskets 22 with different numbers of slots 221 can be arranged as required.

[0039] Specifically, in this embodiment, five slots 221 are specifically provided. Through the arrangement of this structure, simultaneous experimental operations on multiple samples can be performed in the same sample basket 22, thereby improving the use flexibility of the device.

[0040] In this embodiment, it is more preferred that a plurality of protrusions are provided at the bottom of the reagent tank 21 , and the protrusions are used to bear the sample basket 22 .

[0041] It should be noted that the protrusions are used to support the sample basket 22 to prevent the bottom of the sample basket 22 from directly contacting the bottom of the reagent tank 21, thereby improving the fluidity of the liquid at the bottom of the sample basket 22 and making the soaking effect of the root system more uniform.

[0042] In one embodiment, the experimental assembly 2 further includes a drain valve 25 , which is disposed outside the reagent tank 21 to drain the liquid inside the reagent tank 21 .

[0043] The provision of the drain valve 25 can facilitate the operator to drain the liquid in the corresponding reagent tank 21, further optimizing the use flexibility of the device.

[0044] In one embodiment, preferably, in order to facilitate the operator to observe the liquid capacity in the reagent tank 21 , a scale is further provided on the inner wall of the reagent tank 21 .

[0045] In one embodiment, it is more preferred that the experimental assembly 2 further includes an electric heating plate, which is disposed at the bottom of the reagent tank 21 to heat the liquid inside the reagent tank 21 .

[0046] The setting of the electric heating plate allows the operator to heat the liquid in the reagent tank 21 according to the experimental requirements, thereby optimizing the overall practicality of the device.

[0047] The utility model also provides a specific method of using the device:

[0048] The experiment requires clean water, potassium hydroxide, hydrogen chloride and triphenyl blue reagent, which are first injected into each reagent bottle 23;

[0049] Prepare the experimental mycorrhizae, cut each root sample into 1 cm long root segments, put them in a separate mesh chamber, 5 in a group, put them into the corresponding intervals of the sample baskets 22, and put them into the reagent tank 21, and take out the remaining sample baskets 22;

[0050] Add potassium hydroxide solution into the reagent tank 21, adjust the hot plate, and place in a water bath at 90°C for 60 minutes;

[0051] Then, the sample basket 22 is lifted and transferred to the reagent tank 21 corresponding to the clean water, and the sample basket 22 is cooled and the potassium hydroxide solution is washed away;

[0052] After cleaning, transfer to the reagent tank 21 corresponding to the hydrogen chloride reagent and acidify for 5 minutes;

[0053] After acidification, transfer to the reagent tank 21 corresponding to clean water for rinsing;

[0054] After cleaning, transfer to the reagent tank 21 corresponding to the Triphenyl Blue reagent, adjust the electric heating plate, and dye in a 90°C water bath for 30 minutes;

[0055] After staining, the sample was transferred to the reagent tank 21 corresponding to clean water for rinsing to remove the triphenyl blue, and then 1:1 lactic acid glycerol was added to decolorize and prepare the slices at room temperature.

[0056] Reference Figure 2As shown, the utility model also provides another experimental device for batch research of plant mycorrhizal infection rate, which is different from the above embodiment in that a water tank 3 is further provided in the experimental box 1, and the water tank 3 is arranged along the arrangement direction of the experimental components 2 and is correspondingly arranged on the side of the reagent tank 21. A water outlet valve 31 is provided on the water tank 3, and a clean water bottle is also provided in the experimental box 1, and the clean water bottle supplies clean water to the water outlet valve 31.

[0057] In this embodiment, the water tank 3 is integrally arranged at the side of each reagent tank 21. After completing the immersion of the current reagent tank 21, the operator can directly lift the sample basket 22 and put it into the water tank 3 at the side of the current reagent tank 21 for cleaning. During the overall operation, after each reagent immersion, the sample basket 22 needs to be cleaned with clean water. In the process of moving the sample basket 22, when the sample basket 22 passes over the adjacent reagent tank 21, its reagent may contaminate other reagent tanks 21. A separate water tank 3 can avoid this situation. When the sample basket 22 needs to be cleaned, the sample basket 22 can be directly placed in the water tank 3 on one side.

[0058] Among them, it is more preferred that, in order to facilitate cleaning, a water outlet valve 31 is provided in the water tank 3 corresponding to each reagent tank 21. In addition, in order to facilitate pouring out the liquid in the water tank 3, a drain valve 25 is also provided outside the water tank 3.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the utility model.

Claims

1. An experimental device for batch research of plant mycorrhizal infection rate, characterized in that: include: An experimental box, the experimental box is open; and an experimental assembly, the experimental assembly includes a reagent tank, a sample basket and a reagent bottle, the reagent tank is provided with a valve, the valve is connected to the reagent bottle, and the sample basket is arranged in the reagent tank; The experimental components are provided in a plurality and are arranged in the experimental box; A plurality of slots are separately arranged in the sample basket; The experimental components are arranged side by side inside the experimental box; The experimental box is also provided with a water tank, which is arranged along the arrangement direction of the experimental components and correspondingly arranged on the side of the reagent tank; The water tank is provided with a water outlet valve, and the experimental box is also provided with a clean water bottle, which supplies clean water to the water outlet valve.

2. The experimental device according to claim 1, characterized in that: The experimental assembly also includes a drain valve, which is arranged outside the reagent tank and is used to drain the liquid inside the reagent tank.

3. The experimental device according to claim 1, characterized in that: The inner wall of the reagent tank is also provided with scales.

4. The experimental device according to claim 1, characterized in that: The experimental assembly also includes an electric heating plate, which is arranged at the bottom of the reagent tank and is used to heat the liquid inside the reagent tank.

5. The experimental device according to claim 1, characterized in that: A plurality of protrusions are arranged at the bottom of the reagent tank, and the protrusions are used to bear the weight and support the sample basket.

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