Forward focusing mechanism and optical detection device

Abstract

The utility model discloses a forward focusing mechanism and an optical detection device. The forward focusing mechanism comprises a focusing lens assembly; the forward collection lens bracket is provided with a guide groove which extends along the axial direction of the focal lens assembly, and the focal lens assembly is arranged in the guide groove; the adjusting assembly is connected with the forward collecting mirror bracket, and the adjusting assembly drives the focal lens assembly to move in the axial direction of the focal lens assembly relative to the forward collecting mirror bracket. The adjusting assembly enables the focal lens assembly to move in the axial direction of the focal lens assembly so as to adjust the focal length of the focal lens assembly.

Description

Technical Field

[0001] The utility model relates to the technical field of cell analysis equipment, in particular to a forward focusing mechanism and an optical detection device. Background Art

[0002] The laser system in the flow cytometer shines on the fluorescently labeled sample passing through the flow chamber. After the fluorescence is excited, it will emit a forward scattered light signal. In the forward direction, the high-intensity laser needs to be blocked to obtain more effective scattered light signals.

[0003] In the process of collecting forward scattered light, the light beam is focused and converges at the focal point. When the focal point coincides with the fluorescently labeled sample in the flow chamber, the fluorescence will be excited with maximum efficiency. At this time, there are both laser signals and excited fluorescence scattered signals at the sample in the flow chamber (the beam focus position). After these two signals pass through the optical system in the flow chamber, an elliptical light spot will be formed in the forward direction.

[0004] When the focus deviates from the sample, the fluorescence signal after excitation will be significantly weakened, thereby reducing the collection of forward signals and affecting the forward analysis results. However, due to errors in the manufacturing and installation of related parts in the system, there is an error between the focus of the light beam passing through the focusing lens assembly and the sample.

[0005] Therefore, the laser system at the front end of the flow cytometer needs to focus the beam before it hits the sample.

[0006] In the related art, the focusing lens module is installed on a V-shaped block with a guide, and the lens barrel slides in the V-shaped groove. After adjustment, it is fixed at the upper end with a pressure plate. The problem is that the processing accuracy of the V-shaped groove is difficult to ensure, and after installation with the lens barrel, it is difficult to ensure that the initial position is consistent with the designed position, which increases the adjustment range and adjustment time of the lens barrel, cannot meet the needs of fine-tuning, and the adjustment method is not flexible enough. Utility Model Content

[0007] The utility model aims to solve at least one of the technical problems existing in the prior art. To this end, the utility model proposes an adjustment component to move the focusing lens component along the axial direction of the focusing lens component to adjust the focal length of the focusing lens component.

[0008] According to the forward focusing mechanism of the embodiment of the first aspect of the utility model, it includes: a focusing mirror assembly; a forward collecting mirror frame, the forward collecting mirror frame is provided with a guide groove extending along the axial direction of the focusing mirror assembly, and the focusing mirror assembly is arranged in the guide groove; an adjusting assembly, the adjusting assembly is connected to the forward collecting mirror frame, and the adjusting assembly drives the focusing mirror assembly to move relative to the forward collecting mirror frame along the axial direction of the focusing mirror assembly.

[0009] According to the forward focusing mechanism of the embodiment of the utility model, the adjusting assembly enables the focusing mirror assembly to move along the axial direction of the focusing mirror assembly to adjust the focal length of the focusing mirror assembly.

[0010] According to some embodiments of the utility model, the adjustment assembly includes: an adjustment member and a moving member, the moving member is connected to the focusing lens assembly, the adjustment member is connected to the forward collecting lens frame, and the adjustment member drives the moving member to move axially along the focusing lens assembly so that the focusing lens assembly moves along the axial direction of the focusing lens assembly with the moving member.

[0011] According to some embodiments of the present invention, the adjustment assembly further includes: a connecting member, wherein the connecting member is connected to the forward collecting mirror frame, and the connecting member is connected to the forward collecting mirror frame.

[0012] According to some embodiments of the utility model, the adjusting member is a focusing bolt, which comprises: an adjusting portion and a threaded portion, wherein the threaded portion extends along the axial direction of the focusing lens assembly, one end of the threaded portion is connected to the adjusting portion and the other end passes through the threaded hole to abut against the movable member.

[0013] According to some embodiments of the utility model, the forward focusing mechanism also includes: a reset assembly, the reset assembly includes: an elastic part, a movable part and a fixed part, the movable part is fixedly connected to the focusing mirror assembly, the fixed part is fixedly connected to the forward collecting mirror frame, and the elastic part is connected between the movable part and the fixed part and extends along the axial direction of the focusing mirror assembly.

[0014] According to some embodiments of the utility model, a movable hole extending along the axial direction of the focusing lens assembly is provided on the forward collecting lens frame, the movable part passes through the movable hole and is fixedly connected to the focusing lens assembly, and the movable part moves in the movable hole along the axial direction of the focusing lens assembly.

[0015] According to some embodiments of the utility model, the forward focusing mechanism further includes: a first fastener, the inner wall of the guide groove is provided with a matching hole, and the first fastener abuts against the focusing mirror assembly after passing through the matching hole.

[0016] According to some embodiments of the utility model, the adjustment assembly includes: a first sliding member and a second sliding member, the first sliding member and the second sliding member extend along the axial direction of the focusing lens assembly, the first sliding member is connected to the focusing lens assembly, the second sliding member is connected to the forward collecting lens frame, and the first sliding member and the second sliding member are slidably matched.

[0017] According to the optical detection device of the second aspect of the utility model, the embodiment includes: a flow chamber, which is used for the sample to be tested to pass through and provides a place for the sample to be tested to be irradiated by a light source; a light source, which is used to emit light and is incident on the sample to be tested flowing through the flow chamber; and a forward focusing mechanism, which is used to adjust the focus system before irradiating the flow chamber.

[0018] According to some embodiments of the present invention, the optical detection device further comprises: a sample loading device, which is connected to the flow chamber and is used to draw the sample to be tested from the sample tube and transport it to the flow chamber.

[0019] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0021] Figure 1 is a schematic structural diagram of a forward focusing mechanism, a flow chamber and an observation plate according to an embodiment of the utility model;

[0022] Figure 2 It is a schematic structural diagram of a forward focusing mechanism and a flow chamber in one direction according to an embodiment of the utility model;

[0023] Figure 3 is a structural schematic diagram of a forward focusing mechanism and a flow chamber in another direction according to an embodiment of the utility model;

[0024] Figure 4 It is an exploded view of the forward focusing mechanism according to an embodiment of the utility model.

[0025] Reference numerals:

[0026] 10. forward focusing mechanism; 11. focusing mirror assembly; 111. positioning groove; 12. forward collecting mirror frame; 121. guide groove; 122. movable hole; 123. mounting step; 124. matching hole; 13. adjusting assembly; 131. moving part; 132. connecting part; 14. focusing bolt; 141. adjusting part; 142. threaded part; 15. reset assembly; 151. elastic part; 152. movable part; 153. fixing part; 16. first fastener;

[0027] 21. Flow chamber; 22. Observation plate. DETAILED DESCRIPTION

[0028] The embodiments of the present utility model are described in detail below. The embodiments described with reference to the accompanying drawings are exemplary. The embodiments of the present utility model are described in detail below.

[0029] Reference below Figure 1-Figure 4 A forward focusing mechanism 10 according to an embodiment of the present utility model is described, and an optical detection device including the forward focusing mechanism 10 is also proposed.

[0030] Combination Figure 1-Figure 4 As shown, the forward focusing mechanism 10 comprises: a focusing lens assembly 11, a forward collecting lens frame 12 and an adjusting assembly 13. The forward collecting lens frame 12 is provided with a guide groove 121 extending along the axial direction of the focusing lens assembly 11, and the focusing lens assembly 11 is arranged in the guide groove 121. The guide groove 121 is a non-rotating hole, which plays an anti-rotation role for the focusing lens assembly 11.

[0031] The adjusting assembly 13 is connected to the forward collecting mirror frame 12, and the adjusting assembly 13 drives the focusing mirror assembly 11 to move along the axial direction of the focusing mirror assembly 11 relative to the forward collecting mirror frame 12. Specifically, the adjusting assembly 13 is connected to the forward collecting mirror frame 12, and the adjusting assembly 13 can be transmission-connected with the focusing mirror assembly 11, and the adjusting assembly 13 can move the focusing mirror assembly 11 along the axial direction of the focusing mirror assembly 11 relative to the forward collecting mirror frame 12, thereby completing the focusing of the focusing mirror assembly 11.

[0032] After the laser passes through the focusing mirror assembly 11, the light is focused on the sample in the flow chamber 21. The forward collecting mirror frame 12 and the observation plate 22 are respectively arranged on both sides of the focusing adjustment system in front of the flow chamber 21. The observation plate 22 is placed on the side of the focusing adjustment system away from the forward collecting mirror frame 12 to observe the actual spot shape formed after the light is focused on the sample to be tested in the flow chamber 21. When the actual spot shape is consistent with the theoretical spot shape on the observation plate 22, it indicates that the focusing is completed; when the shape is inconsistent, the focusing mirror assembly 11 is moved along the axial direction of the focusing mirror assembly 11 by adjusting the assembly 13, so as to adjust the position of the focusing mirror assembly 11 until the actual spot shape is consistent with the theoretical spot shape. At this time, the focusing is completed.

[0033] Thus, the adjusting assembly 13 enables the focusing lens assembly 11 to move along the axial direction of the focusing lens assembly 11 to adjust the focal length of the focusing lens assembly 11 .

[0034] That is, in the forward focusing mechanism 10 of the embodiment of the utility model, during the initial installation, the focusing mirror assembly 11 is installed in the guide groove 121, limiting the installation accuracy of the focusing mirror assembly 11 in the circumferential direction; in the subsequent installation and adjustment process, it is only necessary to fine-tune the focusing mirror assembly 11 through the adjustment assembly 13 according to the morphological difference of the actual light spot on the observation plate, thereby improving the installation and adjustment efficiency. This makes the forward focusing simple, easy and efficient, and solves the problem that the optical system of the forward focusing mechanism 10 is focused inside the flow chamber and it is difficult to detect the focus position.

[0035] like Figure 1-Figure 4 As shown, the adjustment component 13 includes: an adjustment member and a moving member 131, the moving member 131 is connected to the focusing lens assembly 11, the adjustment member is connected to the forward collecting lens frame 12, and the adjustment member drives the moving member 131 to move along the axial direction of the focusing lens assembly 11, so that the focusing lens assembly 11 moves along the axial direction of the focusing lens assembly 11 with the moving member 131. Specifically, the adjustment member can be connected to the focusing lens assembly 11 through the moving member 131, the moving member 131 and the focusing lens assembly 11 move synchronously, the adjustment member can make the moving member 131 move along the axial direction of the focusing lens assembly 11, and the focusing lens assembly 11 can move along the axial direction of the focusing lens assembly 11 with the moving member 131. That is, the adjustment member can make the focusing lens assembly 11 move along the axial direction of the focusing lens assembly 11.

[0036] Furthermore, the adjustment assembly 13 also includes: a connecting piece 132, the connecting piece 132 is connected to the forward collection mirror frame 12, a threaded hole is provided on the connecting piece 132, and the threaded portion 142 is threadedly connected to the threaded hole. Specifically, the connecting piece 132 is detachably connected to the forward collection mirror frame 12, so as to facilitate the installation of the focusing lens assembly 11 in the guide groove 121. The forward collection mirror frame 12 is provided with a mounting step 123, and the mounting step 123 is arranged on the upper side of the guide groove 121. The connecting piece 132 cooperates with the mounting step 123 and is detachably connected to the forward collection mirror frame 12, and can be screwed.

[0037] In some embodiments, a mounting hole is provided on the connecting member 132 , and the adjustment assembly 13 further includes: a fourth fastener, which passes through the mounting hole and is fixedly connected to the forward collecting mirror frame 12 , thereby fixing the connecting member 132 on the forward collecting mirror frame 12 .

[0038] The connecting member 132 is perpendicular to the axial direction of the focusing lens assembly 11 , so that the focusing bolt 14 passes through the connecting member 132 and abuts against the moving member 131 , so that the focusing bolt 14 adjusts the position of the focusing lens assembly 11 .

[0039] like Figure 4As shown, the adjusting member is a focusing bolt 14. Specifically, the focusing bolt 14 is threadedly connected to the forward collecting lens frame 12, and one end of the focusing bolt 14 is connected to the moving member 131. The focusing bolt 14 is rotated, and the focusing bolt 14 can move along the axial direction of the focusing lens assembly 11. Then, the moving member 131 and the focusing lens assembly 11 move along the axial direction of the focusing lens assembly 11 under the action of the focusing bolt 14 to adjust the focal length of the focusing lens assembly 11.

[0040] The focusing bolt 14 includes: an adjusting portion 141 and a threaded portion 142, the threaded portion 142 extends along the axial direction of the focusing lens assembly 11, the threaded portion 142 is screwed to the forward collecting lens frame 12, one end of the threaded portion 142 is connected to the adjusting portion 141 and the other end passes through the forward collecting lens frame 12 and is connected to the moving member 131. Specifically, the threaded portion 142 is screwed to the forward collecting lens frame 12 so that the focusing bolt 14 moves along the axial direction of the focusing lens assembly 11. When the operator rotates the adjusting portion 141, the focusing bolt 14 gradually moves along the axial direction of the focusing lens assembly 11 under the screwing action of the threaded portion 142 and the forward collecting lens frame 12, and the moving member 131 moves along the axial direction of the focusing lens assembly 11 under the action of the focusing bolt 14, that is, the focusing lens assembly 11 also moves along the axial direction to complete the focusing of the focusing lens assembly 11.

[0041] The outer wall of the focusing lens assembly 11 is provided with a positioning groove 111, and the moving member 131 is positioned and matched with the positioning groove 111. The moving member 131 can be fixedly connected to the focusing lens assembly 11 through a third fastener, so that the focusing lens assembly 11 and the moving member 131 move synchronously. Among them, the third fastener can be a screw.

[0042] The adjustment assembly 13 further includes: a second fastener, which passes through the connecting member 132 and is fixedly connected to the forward collecting mirror frame 12 to fix the connecting member 132 to the forward collecting mirror frame 12. The second fastener can be a screw.

[0043] Furthermore, the forward focusing mechanism 10 further includes: a reset assembly 15, the reset assembly 15 includes: an elastic member 151, a movable member 152 and a fixed member 153, the movable member 152 is fixedly connected to the focusing lens assembly 11, the fixed member 153 is fixedly connected to the forward collecting lens frame 12, and the elastic member 151 is connected between the movable member 152 and the fixed member 153 and extends along the axial direction of the focusing lens assembly 11. Specifically, the movable member 152 is fixedly connected to the focusing lens assembly 11, and the movable member 152 moves along the axial direction with the focusing lens assembly 11; the fixed member 153 is fixedly connected to the forward collecting lens frame 12, and the elastic member 151 is connected between the movable member 152 and the fixed member 153. When the focusing bolt 14 moves toward the flow chamber 21, the focusing mirror assembly 11 moves toward the flow chamber 21. At this time, the movable part 152 moves toward the flow chamber 21, and the elastic part 151 is stretched; when the focusing mirror assembly 11 moves away from the flow chamber 21, the focusing bolt 14 is rotated, and the focusing bolt 14 moves toward the side away from the flow chamber 21. Under the tension of the elastic part 151, the focusing mirror assembly 11 moves with the movable part 152 toward the side away from the flow chamber 21.

[0044] According to some embodiments of the utility model, a movable hole 122 extending along the axial direction of the focusing lens assembly 11 is provided on the forward collecting lens frame 12, and the movable member 152 passes through the movable hole 122 and is fixedly connected to the focusing lens assembly 11, and the movable member 152 moves along the axial direction of the focusing lens assembly 11 in the movable hole 122. In other words, the movable hole 122 extends along the axial direction of the focusing lens assembly 11, and the movable member 152 moves along the axial direction of the focusing lens assembly 11 in the movable hole 122, and the moving range of the focusing lens assembly 11 is smaller than the size of the movable hole 122 in the axial direction, so as to limit the adjustment range of the focusing lens assembly 11.

[0045] In some other embodiments, the adjustment assembly 13 includes: a first sliding member and a second sliding member, the first sliding member and the second sliding member extend along the axial direction of the focusing lens assembly 11, the first sliding member is connected to the focusing lens assembly 11, the second sliding member is connected to the forward collecting lens frame 12, and the first sliding member and the second sliding member are slidably matched. In other words, the focusing lens assembly 11 and the forward collecting lens frame 12 are slidably connected through the first sliding member and the second sliding member, so that the focusing lens assembly 11 can move along the axial direction of the focusing lens assembly 11 relative to the forward collecting lens frame 12, thereby completing the focusing of the focusing lens assembly 11.

[0046] In some embodiments, the forward focusing mechanism 10 further includes: a first fastener 16, and a matching hole 124 is provided on the inner wall of the guide groove 121. The first fastener 16 passes through the matching hole 124 and abuts against the focusing lens assembly 11. In other words, the matching hole 124 extends in the height direction, and the first fastener 16 can pass through the matching hole 124 and abut against the focusing lens assembly 11, so as to fix the positions of the focusing lens assembly 11 and the forward collecting lens frame 12. When the focusing lens assembly 11 is adjusted to a suitable position, it is fixed by the first fastener 16 to prevent the focusing lens assembly 11 from moving axially due to interference from external factors.

[0047] The first fastener 16 may be a screw. If the focusing lens assembly 11 is to be focused, the first fastener 16 is loosened, and the focusing lens assembly 11 is moved to a corresponding position along the axial direction, and the first fastener 16 is tightened at this time to fix the focusing lens assembly 11 to the forward collecting lens frame 12 to prevent the focusing lens assembly 11 from moving axially.

[0048] According to the optical detection device of the second aspect of the utility model, the flow chamber 21 is used for the samples to be tested of the detection sample liquid to queue up and pass through under the influence of the diluent. The flow chamber 21 provides a place for the samples to be tested to be covered by a light source, so that after the light source irradiates the samples to be tested in the flow chamber 21, it is convenient to analyze the information of the samples to be tested.

[0049] The optical detection device further includes: a light source, which is used to emit light, and the optical fiber emitted by the light source is directed toward the sample to be tested flowing through the flow chamber 21, so as to facilitate subsequent analysis of information of the sample to be tested.

[0050] The optical detection device further includes: a forward focusing mechanism 10, which is used to irradiate the focusing adjustment system in front of the flow chamber 21. The light emitted by the light source is focused and the focused light is transmitted to the focusing adjustment system.

[0051] The optical detection device also includes: a sample loading device, which is connected to the sample tube and the flow chamber bracket. The sample loading device absorbs the sample to be tested in the sample tube and transports the sample to be tested to the flow chamber to facilitate the collection and analysis of information of the sample to be tested.

[0052] As a preferred implementation scheme of this embodiment, the working principle of the optical detection device is as follows: the light beam emitted by the laser forms an elliptical light spot at the center of the flow chamber, the short axis direction of the elliptical light spot is consistent with the flow direction of the sample to be tested, and the long axis direction is perpendicular to the flow direction of the sample to be tested; when the sample to be tested passes through the light beam irradiation area (the elliptical light spot at the center of the flow chamber), the forward scattering signal generated by the scattering of the sample to be tested is collected and analyzed to finally obtain the information of the sample to be tested.

[0053] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0054] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example.

[0055] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A forward focusing mechanism, characterized in that: include: Focusing lens assembly (11); A forward collecting mirror frame (12), wherein the forward collecting mirror frame (12) is provided with a guide groove (121) extending along the axial direction of the focusing mirror assembly (11), and the focusing mirror assembly (11) is arranged in the guide groove (121); An adjusting component (13), wherein the adjusting component (13) is connected to the forward collecting mirror frame (12), and the adjusting component (13) drives the focusing mirror assembly (11) to move along the axial direction of the focusing mirror assembly (11) relative to the forward collecting mirror frame (12).

2. The forward focusing mechanism according to claim 1, characterized in that: The adjusting component (13) comprises: an adjusting member and a moving member (131); the moving member (131) is connected to the focusing lens component (11); the adjusting member is connected to the forward collecting lens frame (12); the adjusting member drives the moving member (131) to move along the axial direction of the focusing lens component (11), so that the focusing lens component (11) moves along the axial direction of the focusing lens component (11) with the moving member (131).

3. The forward focusing mechanism according to claim 2, characterized in that: The adjustment assembly (13) further comprises: a connecting member (132), wherein the connecting member (132) is connected to the forward collecting mirror frame (12).

4. The forward focusing mechanism according to claim 3, characterized in that: The adjusting member is a focusing bolt (14), and the focusing bolt (14) comprises: an adjusting portion (141) and a threaded portion (142), wherein the threaded portion (142) extends along the axial direction of the focusing lens assembly (11), and a threaded hole is provided on the connecting member (132), one end of the threaded portion (142) is connected to the adjusting portion (141) and the other end passes through the threaded hole to abut against the moving member (131).

5. The forward focusing mechanism according to claim 1, characterized in that: Also includes: A reset assembly (15), the reset assembly (15) comprising: an elastic member (151), a movable member (152) and a fixed member (153), the movable member (152) being fixedly connected to the focusing lens assembly (11), the fixed member (153) being fixedly connected to the forward collecting lens frame (12), the elastic member (151) being connected between the movable member (152) and the fixed member (153) and extending along the axial direction of the focusing lens assembly (11).

6. The forward focusing mechanism according to claim 5, characterized in that: The forward collecting mirror frame (12) is provided with a movable hole (122) extending along the axial direction of the focusing mirror assembly (11); the movable member (152) passes through the movable hole (122) and is fixedly connected to the focusing mirror assembly (11); and the movable member (152) moves in the movable hole (122) along the axial direction of the focusing mirror assembly (11).

7. The forward focusing mechanism according to claim 1, characterized in that: Also includes: A first fastener (16), the inner wall of the guide groove (121) is provided with a matching hole (124), and the first fastener (16) abuts against the focusing lens assembly (11) after passing through the matching hole (124).

8. The forward focusing mechanism according to claim 1, characterized in that: The adjustment assembly (13) comprises: a first sliding member and a second sliding member, wherein the first sliding member and the second sliding member extend along the axial direction of the focusing lens assembly (11), the first sliding member is connected to the focusing lens assembly (11), the second sliding member is connected to the forward collecting lens frame (12), and the first sliding member and the second sliding member are slidably matched.

9. An optical detection device, characterized in that: include: A flow chamber (21), the flow chamber (21) is used for allowing the sample to be tested to pass through and providing a place for the sample to be tested to be irradiated by a light source; A light source, the light source is used to emit light and incident on the sample to be tested flowing through the flow chamber (21); The forward focusing mechanism (10) as claimed in any one of claims 1 to 8 is used for a focusing adjustment system before irradiating a flow chamber (21).

10. An optical detection device according to claim 9, characterized in that: Also includes: Sample loading device, The sample loading device is connected to the flow chamber (21) and is used to draw the sample to be tested from the sample tube and transport it to the flow chamber (21).

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