Intelligent device for rapidly detecting fruit quality

Abstract

The utility model relates to intelligent equipment for rapidly detecting fruit quality. The intelligent equipment is provided with intelligent detection equipment based on a paper-based micro-fluidic chip and a detection paper-based micro-fluidic chip which is matched with the intelligent detection equipment and can respectively correspond to various different fruit nutrients and develop colors, the intelligent detection equipment comprises a cover body, a box body and a pipette tip storage box, and a shell is provided with a capacitive touch screen, a pipette, a sample buffer solution and a color developing agent; a white light source, a colorimetric groove, a colorimetric detection module and a single chip microcomputer are arranged in the shell, wherein the colorimetric detection module and the single chip microcomputer are sequentially connected. According to the utility model, on-site real-time testing is realized and operation is very convenient; an equipment user interface is simple and clear, operation is easy, and a detection result is visual and clear; the economical efficiency is good; the six fruit nutrients are tested at the same time, and rapidness and simplicity are achieved; the storage box is arranged, so that multiple times of detection can be performed; interference is avoided through image acquisition and data processing in the device; an independent light source is arranged for each detection channel groove, the uniform illumination area is large enough, and accurate detection is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of fruit quality detection, and in particular to intelligent equipment for rapidly detecting fruit quality based on a paper-based microfluidic chip. Background Art

[0002] Fresh fruits play an important role in people's diet. Fruits are rich in sugar, organic acids, proteins, vitamins, total flavonoids, anthocyanins, polyphenols and other ingredients. The antioxidants and phytochemicals in them have great potential benefits. These substances can help remove free radicals in the body, slow down cell aging and oxidative damage, and prevent the occurrence of many chronic diseases. Proper intake of fruits is of great significance to maintaining human health and preventing foodborne diseases. In recent years, with the improvement of people's living standards and the demand for diversified consumption, consumers have higher and higher requirements for fruit quality, from low-level price competition to comprehensive competition of quality, brand and price. Quality has become the factor that consumers pay most attention to, especially the intrinsic quality of fruits.

[0003] Existing methods for detecting the internal quality of fruits mainly include near-infrared spectroscopy, hyperspectral technology, nuclear magnetic resonance technology, dielectric properties technology, etc. The near-infrared spectroscopy detection method has a fast detection speed and does not damage the sample, but it is easily affected by the detection environment. Hyperspectral imaging technology has the advantages of strong detection capability, high image resolution, and rich spectral information, but it cannot perform real-time analysis on site. Nuclear magnetic resonance detection technology has high resolution and accuracy, but the use of this technology is limited by the high cost of equipment. Dielectric properties detection technology has the advantages of simple operation, strong anti-interference ability, and less information redundancy, but its detection sensitivity and accuracy need to be improved. Therefore, it is of great significance to develop a fast, high-throughput, online, and intelligent fruit nutrient component detection equipment. Summary of the invention

[0004] The purpose of the utility model is to overcome the unsatisfactory defects of the equipment and methods for detecting fruit quality in the prior art, such as time-consuming and detection costs, and to provide a fast, high-throughput, online intelligent equipment for rapid detection of fruit and vegetable quality.

[0005] The purpose of this utility model is achieved through the following technical solutions:

[0006] The intelligent equipment for rapid detection of fruit quality includes an intelligent detection equipment based on a paper-based microfluidic chip and a matching detection paper-based microfluidic chip that can correspond to and display colors for various fruit nutrients;

[0007] The intelligent detection equipment includes a cover body, a box body and a pipette tip storage box, and the shell body is provided with a capacitive touch screen, a pipette, a sample buffer and a color developer; the shell body is provided with a white light source, a colorimetric groove, a colorimetric detection module and a single-chip microcomputer connected in sequence;

[0008] A pipette gun, sample buffer and color developer can be placed on the left side of the box body, and a cover body made of an opaque material can be rotated to cover it; a capacitive touch screen is arranged on the right side of the box body; a drawer-type colorimetric groove is arranged on the side of the box body, and an acrylic plate of the same size is covered on it. A white light source is arranged in the box body to illuminate the colorimetric groove directly, and the colorimetric groove is connected to a colorimetric detection module. The colorimetric sensor of the colorimetric detection module is connected to a single-chip microcomputer via an A / D converter, and the capacitive touch screen is connected to the input and output ports of the single-chip microcomputer;

[0009] The single chip microcomputer is arranged on an integrated circuit board, and the integrated circuit board is also provided with a battery charging circuit, a power switch circuit, a buck-boost circuit, a TFT display circuit, a light source circuit and a colorimetric sensor circuit;

[0010] There is a power button and a USB port on the back of the shell that can be used for charging and data transmission.

[0011] In a preferred embodiment, the box body is provided with storage positions for a pipette, a pipette tip, a sample buffer and a color developer.

[0012] In a preferred embodiment, a fracture gap is provided between adjacent channels of the paper-based microfluidic chip.

[0013] Since the channel material is permeable to liquids and the channels are very close to each other, cross-contamination between channels due to fluid penetration will occur, causing the detection to lose accuracy. Therefore, micromachining is used to separate adjacent channels to form gaps in order to achieve the effect of blocking penetration rather than just reducing it.

[0014] In a preferred embodiment, the colorimetric groove has an acrylic plate as a hydrophobic substrate, and another acrylic plate of the same size is covered on top.

[0015] The inherent hydrophobic properties of the acrylic sheet make the boundaries between the hydrophilic and hydrophobic areas clear, allowing the solution to flow accurately from the target channel to the reaction channel, achieving efficient directional wetting and rapid detection. Covering the top with an acrylic sheet of the same size can prevent the paper-based microfluidic chip from moving.

[0016] In a preferred embodiment, the colorimetric groove has a snowflake-like structure and is consistent with the paper-based microfluidic chip.

[0017] The preferred solution is that the colorimetric sensor uses a high-sensitivity color acquisition sensor TCS34725.

[0018] In a preferred embodiment, the paper-based microfluidic chip with a snowflake-like structure is provided with six detection channels corresponding to six fruit nutrients, namely fructose, glucose, sucrose, vitamin C, amino acids and acidity, and reacting with respective color developers respectively.

[0019] In a preferred embodiment, the light source is an incandescent lamp, and a plurality of light sources are arranged in one-to-one correspondence with a plurality of colorimetric grooves.

[0020] Preferably, the white light source illuminates the sample through a condenser, and the uniformly illuminated area is larger than the area of ​​the colorimetric sensor projected on the object through the condenser.

[0021] When constructing a color sensor, the most important things are the lighting source and the focusing system. To effectively form a lighting point on an object, it is most convenient to use an incandescent lamp as a light source. The continuous spectrum characteristics of an incandescent lamp are more adaptable to any color detection than other line spectrum characteristics. As for the focusing system, attention must be paid to the focal length of the condenser and the diameter of the lighting point. The area of ​​the uniform lighting point should be much larger than the area projected on the object by the condenser than the size of the colorimetric sensor. Otherwise, the brightness on the photosensitive element of the colorimetric sensor will be uneven, and the color cannot be distinguished well. If necessary, this intelligent device can be equipped with a light source focus adjustment mechanism.

[0022] Preferably, the intelligent detection equipment is provided with Bluetooth communication that can be connected to a mobile phone.

[0023] The technical principle of the intelligent equipment for rapid detection of fruit quality in this application: This intelligent equipment is based on a paper-based microfluidic chip, and reacts six fruit nutrients, including fructose, glucose, sucrose, vitamin C, amino acids and acidity, with their respective color developers to obtain paper-based microfluidic chips of different colors and grayscales. The paper-based microfluidic chip is placed in a colorimetric groove, and image acquisition and data processing inside the device are used to achieve rapid and accurate detection of fruit quality.

[0024] The application of this intelligent device can realize equipment control, parameter setting, concentration calibration and other tasks through the touch screen.

[0025] The colorimetric sensor converts the received signal into a digital signal and inputs it into the single-chip microcomputer. The single-chip microcomputer system establishes a concentration table based on the collected information and the corresponding concentration, and realizes the detection of fruit quality by establishing a data table model. The single-chip microcomputer integrates the microprocessor and memory on a circuit chip to realize the dual-mode conversion of concentration calibration and real-time detection. The output results of the single-chip microcomputer system are finally displayed on the capacitive touch screen and can also be transmitted to the mobile phone via Bluetooth.

[0026] The beneficial effects of the utility model are:

[0027] 1. On-site real-time testing, very convenient operation;

[0028] 2. The user interface of the equipment is concise and easy to operate, and the test results are intuitive and clear;

[0029] 3. Good economy;

[0030] 4. Test six kinds of fruit nutrients at the same time, fast and simple;

[0031] 5. Equipped with a storage box, which can be tested multiple times;

[0032] 6. Avoid interference through image acquisition and data processing inside the device;

[0033] 7. An independent light source is set for each detection channel groove, and the uniform illumination area is large enough to achieve accurate detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the overall structure appearance of the utility model;

[0035] Figure 2 Schematic diagram of the patterned hydrophobic boundaries of the channels of paper-based microfluidic chips.

[0036] In the figure: detection device 1; paper-based microfluidic chip 2; hydrophobic boundary 3; cover 4; shell 5; pipette tip storage box 6; pipette 7; sample buffer and color developer 8; capacitive touch screen 9; white light source 10; acrylic plate 11; colorimetric groove 12; colorimetric sensor 13; single chip computer 14; microprocessor 15; memory 16; integrated circuit board 17; power button 18; USB port 19. DETAILED DESCRIPTION

[0037] The following is a detailed description of an embodiment of the utility model in conjunction with the accompanying drawings: This embodiment is implemented on the premise of the technical solution of the utility model, and a detailed implementation method and a specific operation process are given, but the protection scope of the utility model is not limited to the following embodiments.

[0038] The purpose of the utility model is achieved through the following technical solutions.

[0039] Embodiment 1: A smart device for rapid detection of fruit quality comprises a detection smart device 1 based on a paper-based microfluidic chip and a matching detection paper-based microfluidic chip 2 that can correspond to and display colors for various fruit nutrients.

[0040] As the core technology of the miniature total analysis system, the paper-based microfluidic chip 2 constructs the biochemical analysis process on a chip of a few square centimeters or even smaller, controls the delivery of reagents through microfluidic channels, and realizes operations such as sample preparation, reaction and detection. Paper-based microfluidic analysis technology uses various micro-fabrication technologies to replace traditional materials with cheap and readily available cellulose filter paper, build a fluid channel network and related analysis devices on the filter paper, and establish a "paper micro-laboratory". The working principle of the paper-based microfluidic chip 2 is to make a patterned hydrophobic boundary 3 on the hydrophilic paper base, thereby forming a microfluidic channel to control the delivery of liquids, perform one or more steps of biochemical reaction processes, and then complete the entire detection and diagnosis process. This high-throughput miniaturized device successfully combines the characteristics of filter paper, which is cheap and easy to obtain, has strong biocompatibility, and is simple to prepare, with the advantages of automation and integration of traditional microfluidic chips. This technology can not only greatly reduce the consumption of expensive reagents, but also double the analysis speed and realize the simultaneous detection of multiple samples. See Figure 1 .

[0041] The intelligent detection equipment 1 includes a cover 4, a box 5 and a pipette tip storage box 6. The housing 5 is provided with a capacitive touch screen 9, a pipette 7, a sample buffer and a color developer 8. The housing 5 is provided with a white light source 10, a colorimetric groove 12, a colorimetric detection module and a single-chip computer 14 connected in sequence. The acrylic plate 11 covers the colorimetric groove 12, and the colorimetric groove 12 is shaped like a snowflake and matches the paper-based microfluidic chip 2. Figure 2 .

[0042] A pipette gun 7, sample buffer and color developer 8 can be placed on the left side of the box body 5, and a cover body 4 made of an opaque material can be rotatably covered on it for light shielding and dust prevention; a capacitive touch screen 9 is provided on the right side of the box body 5; a drawer-type colorimetric groove 12 is provided on the side of the box body 5, and a white light source 10 is provided in the box body 5 to illuminate the colorimetric groove 12. The colorimetric groove 12 is connected to a colorimetric detection module, a colorimetric sensor 13 of the colorimetric detection module is connected to a single-chip microcomputer 14 via an A / D converter, and the capacitive touch screen 9 is connected to the input and output ports of the single-chip microcomputer 14.

[0043] The capacitive touch screen can be used to control the equipment, set parameters, calibrate concentrations, etc. This intelligent equipment integrates Bluetooth communication function to connect to the mobile phone.

[0044] On the integrated circuit board 17 on which the single chip microcomputer 14 is arranged, a battery charging circuit, a power switch circuit, a buck-boost circuit, a TFT display circuit, a light source circuit and a colorimetric sensor 13 circuit are also arranged;

[0045] The single chip computer 14 integrates the microprocessor 15 and the memory 16 on a circuit chip to realize the dual mode conversion of concentration calibration and real-time detection.

[0046] The device is based on the color reaction between the previous sample buffer solution and the specific color developer, combined with the optical colorimetric principle, and the detection system hardware and software adopts a modular design, including detection mode and concentration calibration mode. The colorimetric detection module uses a highly sensitive color acquisition sensor TCS34725 as a photosensitive element, and the white light source 10 passes through the paper-based microfluidic chip 2 that has undergone a color reaction to produce light color absorption. The data acquisition and storage module converts the received signal of the colorimetric sensor 13 into a digital signal and inputs it into the single-chip microcomputer 14. The single-chip microcomputer 14 system establishes a concentration table with the collected information and the corresponding concentration. By establishing a data table model and storing it, qualitative and quantitative detection of fruit nutrients is achieved. The output results of the single-chip microcomputer 14 system are finally displayed on the display screen and can also be transmitted to the mobile phone via Bluetooth.

[0047] A power button 18 and a USB port 19 which can be used for charging or data transmission are provided on the back of the housing 5 .

[0048] A break gap is provided between adjacent channels of the paper-based microfluidic chip 2 to prevent the sample buffer solution from penetrating, and the colorimetric groove 12 is based on an acrylic plate as a hydrophobic substrate, and another acrylic plate 11 is covered on top.

[0049] Since the channel material is permeable to liquids and the channels are very close to each other, cross-contamination will occur between channels due to fluid penetration, causing the detection to lose accuracy. Therefore, micromachining is used to split the adjacent channels to form gaps to achieve the maximum result of preventing penetration. The inherent hydrophobic properties of the acrylic plate make the boundaries between the hydrophilic and hydrophobic areas clear, allowing the solution to flow accurately from the target channel to the reaction channel, achieving efficient directional wetting and rapid detection. Covering the colorimetric groove with an acrylic plate can prevent the movement of the paper-based microfluidic chip.

[0050] The paper-based microfluidic chip 2 with a snowflake-like structure corresponds to six detection channels, which correspond to six fruit nutrients, namely fructose, glucose, sucrose, vitamin C, amino acids and acidity. The pipette 7 is equipped with a pipette tip 6, and the sample buffer solution and a specific color developer 8 are absorbed onto the paper-based microfluidic chip 2 to obtain paper-based microfluidic chips 2 of different colors and grayscales. The paper-based microfluidic chip 2 is placed in the colorimetric groove 12 of the device, and accurate detection of the types and contents of fruit nutrients is achieved through image acquisition and data processing inside the device.

[0051] The white light source 10 illuminates the sample through the condenser lens, and the uniformly illuminated area is larger than the area projected by the colorimetric sensor 13 on the object through the condenser lens.

[0052] Embodiment 2: The light source of this smart device is an incandescent lamp, which is the most convenient light source. In terms of spectrum, incandescent lamps are closest to sunlight than other so-called white light. For example, the effect of illuminating a colored object with a fluorescent lamp is different from the effect of looking at a colored object under sunlight. This smart device is equipped with multiple light sources and is arranged one by one with the same multiple colorimetric grooves. The rest of the structure is the same as that of Embodiment 1.

[0053] When constructing the colorimetric sensor 13, the most important thing is the illumination light source and the focusing system, which should effectively form an illumination point on the object. As for the focusing system, attention must be paid to the focal length of the condenser and the diameter of the illumination point. The uniformly illuminated area should be much larger than the area projected on the object by the condenser of the size of the colorimetric sensor 13. Otherwise, the brightness on the color discrimination element of the colorimetric sensor 13 will be uneven, and the color cannot be well distinguished. If necessary, the intelligent device can be equipped with a light source focus adjustment mechanism to achieve not only detection, but also fine and accurate detection.

[0054] Embodiment 3: The colorimetric sensor 13 uses 2S4C as the color discrimination element. The rest of the structure is the same as that of Embodiment 1 or Embodiment 2.

[0055] The operation process of the present application is as follows: turn on the power 18, open the cover 4, install the pipette 7 with the gun head 6, and respectively absorb the sample buffer and the specific color developer 8 to be tested and drip them on the six channels of the paper-based microfluidic chip 2. Put the paper-based microfluidic chip 2 into the colorimetric groove 12, cover it with the acrylic plate 11, and push the box body 5. The white light source 10 in the box body 5 passes through the paper-based microfluidic chip 2 where the color development reaction has occurred, and the colorimetric sensor 13 absorbs and generates a light signal. The microprocessor 15 and the memory 16 convert the received light signal into a digital signal and input it into the single-chip microcomputer 14 on the integrated circuit board 17. The single-chip microcomputer 14 system establishes a concentration table with the collected information and the corresponding concentration. By establishing a data table model and storing it, the qualitative and quantitative detection of fruit nutrients is realized. The output results of the single-chip microcomputer 14 system are finally displayed on the capacitive touch screen 9, and can also be transmitted to the mobile phone via Bluetooth.

[0056] The above shows and describes the basic principles, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only for explaining the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. An intelligent device for rapid detection of fruit quality, characterized in that: There is an intelligent detection device based on a paper-based microfluidic chip and a matching detection paper-based microfluidic chip that can correspond to and display colors for various fruit nutrients; The intelligent detection equipment includes a cover body, a box body and a pipette tip storage box, and the shell body is provided with a capacitive touch screen, a pipette, a sample buffer and a color developer; the shell body is provided with a white light source, a colorimetric groove, a colorimetric detection module and a single-chip microcomputer connected in sequence; A pipette gun, sample buffer and color developer can be placed on the left side of the box body, and a cover body made of an opaque material can be rotated to cover it; a capacitive touch screen is arranged on the right side of the box body; a drawer-type colorimetric groove is arranged on the side of the box body, and an acrylic plate of the same size is covered on it. A white light source is arranged in the box body to illuminate the colorimetric groove directly, and the colorimetric groove is connected to a colorimetric detection module. The colorimetric sensor of the colorimetric detection module is connected to a single-chip microcomputer via an A / D converter, and the capacitive touch screen is connected to the input and output ports of the single-chip microcomputer; The single chip microcomputer is arranged on an integrated circuit board, and the integrated circuit board is also provided with a battery charging circuit, a power switch circuit, a buck-boost circuit, a TFT display circuit, a light source circuit and a colorimetric sensor circuit; There is a power button and a USB port on the back of the shell that can be used for charging and data transmission.

2. According to claim 1, the intelligent equipment for rapid detection of fruit quality is characterized in that: The box body is provided with storage positions for a pipette, a pipette tip, a sample buffer and a color developer.

3. The intelligent equipment for rapid detection of fruit quality according to claim 1 is characterized in that: A fracture gap is arranged between adjacent channels of the paper-based microfluidic chip.

4. The intelligent equipment for rapid detection of fruit quality according to claim 1 is characterized in that: The colorimetric groove uses an acrylic plate as a hydrophobic base, and another acrylic plate of the same size is covered on top.

5. The intelligent equipment for rapid detection of fruit quality according to claim 1 is characterized in that: The shape of the colorimetric groove is a snowflake-like structure and is consistent with the paper-based microfluidic chip.

6. The intelligent equipment for rapid detection of fruit quality according to claim 1 is characterized in that: The colorimetric sensor uses the highly sensitive color acquisition sensor TCS34725.

7. The intelligent equipment for rapid detection of fruit quality according to claim 1 is characterized in that: The snowflake-shaped paper-based microfluidic chip has six detection channels corresponding to the six fruit nutrients: fructose, glucose, sucrose, vitamin C, amino acids and acidity, which react with their respective color developers.

8. The intelligent equipment for rapid detection of fruit quality according to claim 1 is characterized in that: The light source is an incandescent lamp, and a plurality of light sources are arranged in one-to-one correspondence with a plurality of colorimetric grooves.

9. The intelligent equipment for rapid detection of fruit quality according to claim 1 is characterized in that: A white light source illuminates the sample through a condenser, and the uniformly illuminated area is larger than the area projected on the object by the condenser than the size of the colorimetric sensor.

10. The intelligent equipment for rapid detection of fruit quality according to claim 1 is characterized in that: The detection intelligent equipment is provided with Bluetooth communication which can be connected with a mobile phone terminal.

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