Efficient and safe EMS treatment device for mutating rice seeds

Abstract

The utility model discloses a device for efficient and safe EMS treatment to mutate rice seeds, which relates to the technical field of seed mutation, and comprises a soaking box, a seed soaking frame, a shaking mechanism and a spraying and washing mechanism, the soaking box is divided into an EMS soaking area and a clear water soaking area, the EMS soaking area and the clear water soaking area are separated by a middle arched partition plate, and the spraying and washing mechanism is arranged on the seed soaking frame. The center of the soaking box is rotationally sleeved with a center rotating shaft located above the arched partition plate, when rice seeds are suddenly soaked, automatic position changing of the seed soaking frame in a clear water soaking area and an EMS soaking area can be achieved through a stepping motor and a rotating frame, the seed soaking frame can further automatically move to the position below the spraying and flushing mechanism to be automatically flushed, and the rice seed soaking efficiency is improved. According to the seed treatment device, the residual chemical mutagenic agent is effectively removed, the purity of seeds is guaranteed, manual operation is reduced through automatic control, the seed treatment efficiency is greatly improved, the chance that operators make direct contact with the chemical mutagenic agent is reduced, and the safety risk in the operation process is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of seed mutation, and particularly relates to a device for efficiently and safely treating rice seeds with EMS to mutate them. Background Technique

[0002] The mutation of rice seeds is a process of artificially intervening and using physical or chemical mutagens to induce gene mutations in seeds, aiming to produce rice plants with new traits, and then cultivating new varieties with excellent characteristics through screening and selection. EMS (ethyl methane sulfonate) is a commonly used chemical mutagen, which can cause mutations in the DNA of the organism's genome and is often used to create genetic polymorphisms in plant breeding. In the traditional rice breeding process, seed mutation treatment is a key step, which usually uses chemical mutagens such as ethyl methane sulfonate (EMS) to treat seeds to induce gene mutations. At present, there are still some deficiencies in treating rice seed mutations: traditional methods often require manual operation, with high labor intensity, and it is difficult to ensure the consistency and repeatability of the treatment process. Moreover, the treatment efficiency is low, making it difficult to meet the needs of large-scale breeding; the seeds may not be evenly exposed to the drug during soaking, affecting the mutation effect; the chemical mutagen is toxic and carcinogenic, and there is a risk of operator exposure in the traditional operation process. To solve the above problems, the utility model proposes a device for efficiently and safely treating rice seeds with EMS to mutate them, aiming to improve the efficiency, safety and uniformity of mutation treatment. Content of the Utility Model

[0003] Aiming at the problems existing in the prior art, the utility model provides a device for efficiently and safely treating rice seeds with EMS to mutate them, which can realize the automatic transposition of seeds in the soaking area through automatic control, reduce manual participation, improve the efficiency and safety of operation, and the seeds can be shaken reciprocally during soaking, improve the uniformity of contact with the medicament, and ensure the mutation effect.

[0004] The solution adopted by the present utility model to solve its technical problems is as follows: An efficient and safe EMS device for treating mutant rice seeds, including a soaking tank, further comprising a seed soaking frame, a shaking mechanism, and a spraying and rinsing mechanism. The soaking tank is divided into an EMS soaking area and a clear water soaking area, which are separated by an arched partition plate in the middle. A central rotating shaft located above the arched partition plate is rotatably sleeved at the center of the soaking tank, and a stepping motor for controlling the rotation of the central rotating shaft is installed on the soaking tank. A rotating frame is fixedly sleeved on the central rotating shaft, and the centers of both ends of the seed soaking frame are hinged to the rotating frame. The shaking mechanism includes a transmission shaft rotatably sleeved in the EMS soaking area and the clear water soaking area. A V-shaped frame is fixedly sleeved on the transmission shaft. Push rods are arranged at both ends of the V-shaped frame, and the push rods are located below the seed soaking frame. A full gear is fixedly sleeved at the outer end of the transmission shaft. A toothed plate is horizontally slidably sleeved on the outer wall of the soaking tank. A second rack segment meshing with the full gear is arranged on the toothed plate, and a driving component for controlling the left and right movement of the toothed plate is arranged on the soaking tank. The spraying and rinsing mechanism includes a spray disc installed above the EMS soaking area through a spraying frame. Nozzles are evenly distributed below the spray disc, and a liquid inlet pipe is connected to the spray disc.

[0005] Further, the driving component includes a servo motor installed below the arched partition plate. The output end of the servo motor is fixedly sleeved with a half gear through a driving shaft. The middle of adjacent toothed plates is connected through a straight groove member. A first rack segment is horizontally arranged up and down in the straight groove member, and the first rack segment can mesh with the half gear.

[0006] Further, the liquid inlet pipe includes a clear water pipe and a neutralizing agent pipe, and pumps are installed on both the clear water pipe and the neutralizing agent pipe.

[0007] Further, horizontal sliding grooves are opened at positions on the outer wall of the soaking tank corresponding to the EMS soaking area and the clear water soaking area. Sliders matching the sliding grooves are arranged at the rear side of the toothed plate, and the toothed plate is horizontally slidably sleeved in the sliding grooves through the sliders.

[0008] Further, a counterweight is arranged at the bottom of the seed soaking frame.

[0009] Further, a layer of rubber is coated on the surface of the push rod.

[0010] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0011] The utility model realizes the automatic transposition of the seed soaking frame between the clean water soaking area and the EMS soaking area through a stepping motor and a rotating frame when rice seeds are soaked and mutated. The seed soaking frame can also automatically move under the spray rinsing mechanism for automatic rinsing, effectively removing the residual chemical mutagen, ensuring the purity of the seeds. Through automatic control, manual operation is reduced, the efficiency of seed treatment is greatly improved, and the chance for operators to directly contact the chemical mutagen is reduced, thus lowering the safety risk during the operation process;

[0012] By setting a swaying mechanism, during the soaking and mutation process of the seeds, the servo motor operates. Through a series of transmission cooperations, it can control the push rod to rotate left and right reciprocally, thereby pushing the seed soaking frame to sway left and right in the soaking area, ensuring that the seeds are evenly medicated and cleaned in the treatment liquid. Through uniform soaking and cleaning, the consistency of the mutation effect is guaranteed, and the success rate of seed mutation is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 is a three-dimensional structural schematic diagram of the utility model;

[0014] Figure 2 is a front structural schematic diagram of the utility model;

[0015] Figure 3 is a three-dimensional sectional structural schematic diagram of the utility model;

[0016] Figure 4 is a front sectional structural schematic diagram of the utility model.

[0017] In the figure: 1, soaking tank; 11, EMS soaking area; 12, clean water soaking area; 13, arched partition board; 2, central rotating shaft; 3, stepping motor; 4, rotating frame; 5, seed soaking frame; 51, counterweight; 6, swaying mechanism; 61, transmission shaft; 62, V-shaped frame; 63, push rod; 64, servo motor; 65, driving shaft; 66, semi-gear; 67, toothed plate; 68, straight groove member; 69, first rack section; 610, second rack section; 611, full gear; 612, sliding groove; 7, spray rinsing mechanism; 71, spray rack; 72, spray disc; 73, clean water pipe; 74, neutralizing agent pipe; 75, pump. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0018] The following further describes the utility model in conjunction with the drawings and embodiments.

[0019] Please refer to Figures 1-4 , the utility model provides a technical solution for a device for highly efficient and safe EMS treatment of mutated rice seeds: Embodiment

[0020] according to Figure 1 and Figure 3 As shown, an efficient and safe device for EMS treatment to mutate rice seeds includes a soaking box 1, a seed soaking frame 5 and a spraying and flushing mechanism 7. The soaking box 1 is divided into an EMS soaking area 11 and a clean water soaking area 12. The EMS soaking area 11 and the clean water soaking area 12 are separated by an arched partition plate 13 in the middle of the soaking box 1. An EMS chemical mutagen is arranged in the EMS soaking area 11. Drain pipes are arranged in both the EMS soaking area 11 and the clean water soaking area 12. The spraying and flushing mechanism 7 is located above the EMS soaking area 11. A central shaft 2 located above the arched partition plate 13 is rotatably mounted at the center of the soaking box 1. A stepper motor 3 is installed on the soaking box 1. The output end of the stepper motor 3 is connected to the central shaft 2 and can drive the central shaft 2 to rotate. A rotating frame 4 is fixedly mounted on the central shaft 2. The centers of both ends of the seed soaking frame 5 are hinged on the rotating frame 4. Figure 4 As shown, when the central shaft 2 drives the rotating frame 4 to rotate, the rotating frame 4 can drive the seed soaking frame 5 to rotate around the central shaft 2, so that the seed soaking frame 5 can be exchanged between the EMS soaking area 11 and the clean water soaking area 12, and the seed soaking frame 5 can also be rotated to the bottom of the spray flushing mechanism 7 for flushing. A frame cover is provided on the seed soaking frame 5. The frame cover can be opened to take out and put rice seeds. A counterweight body 51 is provided at the bottom of the seed soaking frame 5. The counterweight body 51 can ensure that the seed soaking frame 5 is always in a horizontal state, thereby improving the stability of the seed soaking frame 5 during rotation, exchange and flushing.

[0021] The spray flushing mechanism 7 includes a spray rack 71 arranged on the side of the EMS soaking area 11, and a spray disc 72 located above the EMS soaking area 11 is installed on the top of the spray rack 71, and nozzles are evenly distributed below the spray disc 72. A liquid inlet pipe is connected to the spray disc 72, and the liquid inlet pipe includes a clean water pipe 73 and a neutralizer pipe 74. Pumps 75 are installed on the clean water pipe 73 and the neutralizer pipe 74. When the seeds are soaked in the EMS soaking area 11, the seed soaking frame 5 can be driven by the central shaft 2 and the rotating frame 4 to move from the EMS soaking area 11 to the bottom of the spray disc 72. By connecting the clean water pipe 73 and the neutralizer pipe 74 to clean water or neutralizer respectively, the spray disc 72 can spray clean water or neutralizer to rinse the rice seeds soaked in the chemical mutagen to wash away the EMS on the seeds. The sprayed clean water or neutralizer flows directly into the EMS soaking area 11, and is subsequently processed and discharged together with the agent in the EMS soaking area 11.

[0022] All components in the device can be controlled by an external controller. The device only needs to manually take the seeds out of the seed soaking frame 5 before and after the seeds are soaked. The rest of the operations can be controlled outside the soaking room, which reduces the time workers are in contact with EMS and improves safety.

[0023] In specific use, for the device of the utility model for efficiently and safely treating mutant rice seeds by EMS, first, dry rice seeds to be mutated are placed into the seed soaking frame 5. Then, the seed soaking frame 5 is rotated into the clear water soaking area 12 by the stepper motor 3 and the rotating frame 4. The dry seeds are soaked in the clear water soaking area 12 at room temperature for 16 h, and the water is filtered. After soaking in clear water, the stepper motor 3 rotates counterclockwise by 90°, controlling the seed soaking frame 5 to move out of the clear water soaking area 12. The seed soaking frame 5 stays above the clear water soaking area 12 for water filtration. After the water filtration is completed, the stepper motor 3 rotates counterclockwise again by 180°, controlling the seed soaking frame 5 to move into the EMS soaking area 11, and soaked with a certain concentration of EMS solution (the EMS solution is prepared with 0.1 mol / L phosphate buffer, pH value 7.2) for 8 h. Then, the stepper motor 3 rotates clockwise by 90°, controlling the seed soaking frame 5 to rotate under the spraying disc 72. The clear water pipe 73 is connected to an external water source, and the corresponding pump 75 is started. Water is sprayed onto the seed soaking frame 5 through the spraying disc 72. The seeds are continuously rinsed with clear water to wash away the EMS on the seeds. After the rinsing is completed, the seed soaking frame 5 can also be controlled to move into the clear water soaking area 12 again, and soaked for 24 - 48 h as appropriate to complete the mutation work of the rice seeds. Example

[0024] Based on Example 1, as Figure 2 and Figure 3 shown, a shaking mechanism 6 is arranged in the MES soaking area and the clear water soaking area 12. The shaking mechanism 6 includes a transmission shaft 61 rotatably sleeved at the bottom of the EMS soaking area 11 and the clear soaking area. Two groups of V-shaped frames 62 are symmetrically and fixedly sleeved on the transmission shaft 61 in the front and back. Push rods 63 are fixed at both ends of the V-shaped frames 62. From Figure 4 it can be seen that when the seed soaking frame 5 is in the EMS soaking area 11 or the clear water soaking area 12, the push rod 63 is directly below the seed soaking frame 5. Since the center of the seed soaking frame 5 is hinged on the rotating frame 4, the transmission shaft 61 drives the push rod 63 to swing left and right, which can push the seed soaking frame 5 to swing left and right around the central axis, causing the seed soaking frame 5 to slightly swing left and right in the chemical mutagen solution or clear water, preventing the seeds from accumulating together and affecting the soaking and absorption effect. Further, a layer of rubber is coated on the outer surface of the push rod 63 to achieve flexible contact between the push rod 63 and the seed soaking frame 5.

[0025] From Figure 2It can be seen that a full gear 611 is fixedly sleeved on the outer end of the transmission shaft 61. A toothed plate 67 is horizontally slidably sleeved on the outer wall of the soaking tank 1. Horizontal sliding grooves 612 are formed in the outer wall of the soaking tank 1 at positions corresponding to the EMS soaking area 11 and the clear water soaking area 12. A slider matching the sliding groove 612 is arranged at the rear side of the toothed plate 67. The toothed plate 67 is horizontally slidably sleeved in the sliding groove 612 through the slider. A second rack segment 610 meshing with the full gear 611 is arranged on the toothed plate 67. A servo motor 64 is installed below the arched partition plate 13 through a motor bracket. A half gear 66 is fixedly sleeved on the output end of the servo motor 64 through a drive shaft 65. Two adjacent toothed plates 67 are connected through a straight groove member 68, and a first rack segment 69 is horizontally arranged up and down in the straight groove member 68, and the first rack segment 69 can mesh with the half gear 66. The driving assembly composed of the servo motor 64, the half gear 66 and the first rack segment 69 can control the left and right sliding of the toothed plate 67, and then drive the transmission shaft 61 to rotate reciprocally under the meshing drive of the full gear 611 and the second rack segment 610, realizing the left and right reciprocating swing of the V-shaped frame 62 to push the seed soaking frame 5 to swing left and right.

[0026] During specific use, for the device for efficiently and safely treating mutant rice seeds with EMS of the present invention, when the seeds are soaked in the clear water soaking area 12 or the EMS soaking area 11, the operation of the servo motor 64 can be controlled to drive the rotation of the half gear 66. Under the meshing action of the half gear 66 with the upper and lower first rack segments 69 in the straight groove member 68, the left and right reciprocating sliding of the two side toothed plates 67 can be controlled. Under the meshing effect of the second rack segment 610 and the full gear 611 on the toothed plate 67, the transmission shaft 61 can be controlled to drive the V-shaped frame 62 to swing left and right reciprocally, and then the seed soaking frame 5 can be pushed to swing left and right reciprocally in the soaking area through the two side push rods 63, so that the seeds can be fully and evenly contacted with the soaking liquid, improving the seed soaking effect.

[0027] The above are only the preferred embodiments of the present invention, which do not limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An efficient and safe device for EMS treatment of rice seeds for mutation, comprising a soaking box (1), characterized in that: It also comprises a seed soaking frame (5), a shaking mechanism (6) and a spraying and flushing mechanism (7); the soaking box (1) is divided into an EMS soaking area (11) and a clean water soaking area (12), the two being separated by an arched partition plate (13) in the middle; the soaking box (1) is centrally rotatably mounted with a central rotating shaft (2) located above the arched partition plate (13); a stepping motor (3) for controlling the rotation of the central rotating shaft (2) is installed on the soaking box (1); a rotating frame (4) is fixedly mounted on the central rotating shaft (2); the centers of both ends of the seed soaking frame (5) are hinged on the rotating frame (4); the shaking mechanism (6) comprises a transmission shaft (61) rotatably mounted on the EMS soaking area (11) and the clean water soaking area; the transmission shaft (61) A V-shaped frame (62) is fixedly mounted on the upper part, push rods (63) are arranged at both ends of the V-shaped frame (62), and the push rods (63) are located below the seed soaking frame (5); a full gear (611) is fixedly mounted on the outer end of the transmission shaft (61); a toothed plate (67) is horizontally slidably mounted on the outer wall of the soaking box (1); a second rack segment (610) meshing with the full gear (611) is arranged on the toothed plate (67), and a driving component for controlling the left and right movement of the toothed plate (67) is arranged on the soaking box (1); the spraying and flushing mechanism (7) comprises a spray plate (72) mounted above the EMS soaking area (11) through a spray frame (71), spray heads are evenly distributed below the spray plate (72), and a liquid inlet pipeline is connected to the spray plate (72).

2. The device for efficiently and safely treating rice seeds with EMS to mutate them according to claim 1, characterized in that: The driving assembly comprises a servo motor (64) installed below the arched partition plate (13); the output end of the servo motor (64) is fixedly sleeved with a half gear (66) via a driving shaft (65); adjacent gear plates (67) are connected in the middle via a straight groove member (68); a rack segment 1 (69) is horizontally arranged in the upper and lower parts of the straight groove member (68); and the rack segment 1 (69) can mesh with the half gear (66).

3. The device for efficiently and safely treating rice seeds with EMS to mutate them according to claim 1, characterized in that: The liquid inlet pipeline comprises a clean water pipe (73) and a neutralizer pipe (74), and a pump (75) is installed on both the clean water pipe (73) and the neutralizer pipe (74).

4. The device for efficiently and safely treating rice seeds with EMS to mutate them according to claim 1, characterized in that: A slide groove (612) is horizontally provided at a position of the outer wall of the soaking box (1) corresponding to the EMS soaking area (11) and the clean water soaking area (12); a sliding block matching the slide groove (612) is provided on the rear side of the tooth plate (67); the tooth plate (67) is horizontally slidably mounted in the slide groove (612) via the sliding block.

5. The device for efficiently and safely treating rice seeds with EMS to mutate them according to claim 1, characterized in that: A counterweight (51) is provided at the bottom of the seed soaking frame (5).

6. The device for efficiently and safely treating rice seeds with EMS to mutate them according to claim 1, characterized in that: The surface of the push rod (63) is coated with a layer of rubber.

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