Plant disease field detection pen

Abstract

The utility model provides a plant disease field detection pen which comprises a body, a transparent door and a display screen which are arranged on the outer surface of the body, a first accommodating space arranged above the body, an opening arranged above the first accommodating space, a first flow guide hole arranged below the first accommodating space, and a second accommodating space arranged below the first flow guide hole and used for placing a PCR (Polymerase Chain Reaction) tube, a first flow guide hole is formed in the first containing space, a microneedle patch is arranged below the body, a rotary sealing mechanism is further arranged in the second containing space and comprises a sealing disc, the sealing disc is tightly close to the lower portion of the first flow guide hole, and a second flow guide hole is formed in the position, corresponding to the first flow guide hole, of the sealing disc. The microneedle patch for collecting plant information is integrated, a PCR (polymerase chain reaction) tube placing space is built in, and a color sensor, a display screen and the like are integrated, so that the defects that traditional equipment is many, miscellaneous and not easy to carry are overcome, and the portability of the equipment is improved.

Description

Technical Field

[0001] The utility model relates to the field of plant disease detection, in particular to a plant disease on-site detection pen. Background Art

[0002] In plants, signs of diseases usually occur on leaves, fruits, buds and young branches. This condition can lead to wasting (falling) or damaged fruits. In addition, these diseases lead to the spread of new infections and diseases, such as seasonal conditions. Therefore, it is very important to identify the disease in advance and take necessary preventive measures before it spreads to other trees. Therefore, fighting plant diseases is the single most important issue in agriculture. There are many types of diseases that affect plants, each of which can cause economic, social and ecological losses. In this context, timely and accurate diagnosis of plant diseases plays an important role in preventing losses in yield and quantity of agricultural products.

[0003] The detection of plant diseases has traditionally relied on manual chemical experimental methods. The process involves not only a wide variety of chemical equipment, but also often requires professionals to go to the site in person to assess the health of the plants. However, carrying a large number of reaction equipment to the site not only increases the risk of loss, but also greatly limits the portability of the equipment, bringing many inconveniences to the detection work. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a plant disease on-site detection pen to solve the problem of plant disease on-site detection.

[0005] In order to solve the above technical problems, the present invention provides a plant disease on-site detection pen, including a main body, a transparent door and a display screen are provided on the outer surface of the main body, a first accommodating space is provided above the main body, an opening is provided above the first accommodating space, a first guide hole is provided below the first accommodating space, a second accommodating space for placing a PCR tube is provided below the first guide hole, a positioning groove is provided in the second accommodating space, the PCR tube is placed in the positioning groove, the second accommodating space corresponds to the transparent door, and a detachable microneedle patch is provided below the main body.

[0006] In a preferred solution, a rotating sealing mechanism is further provided in the second accommodating space, and the rotating sealing mechanism includes a sealing disk, and the sealing disk is tightly placed below the first guide hole. A second guide hole is provided at a position of the sealing disk corresponding to the first guide hole. When the second guide hole corresponds to the first guide hole, the liquid in the first accommodating space can flow into the PCR tube. The rotating sealing mechanism includes a mounting plate, and a DC servo motor is provided below the mounting plate. A rotating disk is connected to the protruding shaft of the DC servo motor, and positioning grooves are evenly distributed on the rotating disk. The PCR tube is placed in the positioning grooves, and the sealing disk is coaxially installed above the rotating disk. A clamping mechanism is provided at the center of the sealing disk and the rotating disk. The clamping mechanism can float up and down to press the sealing disk tightly below the first guide hole.

[0007] In a preferred embodiment, the clamping mechanism includes a fixed seat connected to the rotating disk and a movable rod fixed on the sealing disk. A socket with a first boss is provided in the fixed seat, and a fourth groove matching the first boss is provided on the movable rod. The movable rod can be inserted into the socket, and a spring is also provided between the movable rod and the fixed seat.

[0008] In a preferred solution, a first groove is provided on the outer ring of a side of the sealing disk in contact with the lower side of the first guide hole, a second groove is provided around the second guide hole, and sealing rings are provided in the first groove and the second groove.

[0009] In a preferred solution, a circular third groove is further provided below the main body, a magnet is provided in the third groove, and an iron plate is provided above the microneedle patch.

[0010] In a preferred solution, a third accommodating space is further provided in the main body, and a color sensor is provided in the third accommodating space. The color sensor is electrically connected to the controller, and the color sensor is used to detect the color of the liquid in the PCR tube.

[0011] In a preferred embodiment, a cap for covering the microneedle patch is provided below the main body, and a cover plate for covering the opening is provided above the main body.

[0012] In a preferred solution, a fourth accommodating space is provided in the cap, a third flow guide hole is provided on the side wall of the cap, a through hole is provided below the third flow guide hole, the through hole is used to connect the third flow guide hole and the fourth accommodating space, a fourth flow guide hole is also provided on the side wall of the main body, the fourth flow guide hole corresponds to the third flow guide hole, and a second boss is provided on the cover plate, and the second boss can be inserted into the fourth flow guide hole.

[0013] The beneficial effects of the present invention are as follows: since the present invention is provided with a plant disease on-site detection pen, by integrating a microneedle patch for collecting plant information, a solution reaction accommodating space, a built-in PCR tube placement space, an integrated color sensor, a display screen, etc., the disadvantages of traditional equipment that are numerous and difficult to carry are replaced, the portability of the equipment is improved, the detection is convenient and fast, the results are obtained on-site, it is compatible with the mainstream LAMP color development reaction kit, the sampling and DNA extraction process is simple, and contamination between samples is not easy. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present invention will be further described below in conjunction with the accompanying drawings and embodiments:

[0015] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present invention;

[0016] Figure 2 It is a cross-sectional schematic diagram of the main body and internal structure of an embodiment of the present invention;

[0017] Figure 3 It is a structural schematic diagram of a rotary sealing mechanism according to an embodiment of the present invention;

[0018] Figure 4 is a cross-sectional schematic diagram of a rotary sealing mechanism according to an embodiment of the present invention;

[0019] Figure 5 It is a cross-sectional schematic diagram of the installation of the fixing base and the moving rod according to an embodiment of the present invention;

[0020] Figure 6 This is a structural diagram of a microneedle patch according to an embodiment of the present invention;

[0021] Figure 7 is a cross-sectional schematic diagram of a cap according to an embodiment of the present invention;

[0022] Figure 8 A bottom view of the main body of the embodiment of the present invention;

[0023] Fig. 9 This is a structural diagram of a transparent door according to an embodiment of the present invention;

[0024] Fig.10 It is a structural diagram of the upper part of the main body of an embodiment of the present invention;

[0025] Fig.11 This is a structural diagram of a disposable container according to an embodiment of the present invention;

[0026] Fig.12 This is a structural diagram of a disposable container installed in a first accommodation space according to an embodiment of the present invention.

[0027] Reference numerals: body 1; transparent door 11; third boss 111; display screen 12; first accommodating space 13; opening 131; first guide hole 132; boss 133; bayonet 134; second accommodating space 14; third accommodating space 15; third groove 16; magnet 17; fourth guide hole 18; fourth boss 19; notch 191; rotary sealing mechanism 2; mounting plate 21; DC servo motor 22; rotating disk 23; sealing disk 24; second guide hole 241 ; second groove 242; first groove 243; extending mouth 244; positioning groove 231; clamping mechanism 3; fixing seat 31; first boss 311; plug hole 312; moving rod 32; fourth groove 321; spring 33; microneedle patch 4; microneedle 42; bottom plate 43; buckle 44; PCR tube 51; iron plate 52; cap 6; fourth accommodating space 61; third guide hole 62; through hole 63; cover plate 7; second boss 71; disposable container 8; guide tube 81. DETAILED DESCRIPTION

[0028] Embodiment 1:

[0029] See also Figure 1-5 As shown, the embodiment of the present application provides a technical solution: comprising a body 1, a transparent door 11 and a display screen 12 are provided on the outer surface of the body 1, a first accommodating space 13 is provided above the body 1, an opening 131 is provided above the first accommodating space 13, a first guide hole 132 is provided below the first accommodating space 13, a second accommodating space 14 for placing a PCR tube 51 is provided below the first guide hole 132, the second accommodating space 14 corresponds to the transparent door 11, a heating plate and a temperature sensor are further provided in the second accommodating space 14, and a heating plate and a temperature sensor are further provided in the body 1. A power supply and a controller are provided, and the power supply, the heating plate, and the temperature sensor are electrically connected to the controller. A detachable microneedle patch 4 is provided under the main body 1, and a rotating sealing mechanism 2 is also provided in the second accommodating space 14. The rotating sealing mechanism 2 includes a sealing disk 24, and the sealing disk 24 is closely placed under the first guide hole 132. A second guide hole 241 is provided at a position corresponding to the sealing disk 24 and the first guide hole 132. When the second guide hole 241 corresponds to the first guide hole 132, the liquid in the first accommodating space 13 can flow into the PCR tube 51.

[0030] The transparent door 11 can be made of glass or transparent material, so as to facilitate the observation of the color change of the PCR tube 3 in the body 1 from the outside. When the transparent door 11 is opened, the PCR tube 51 can be placed in the second accommodation space 14.

[0031] Buttons are provided on the display screen 12 , including a power on button, a button for controlling the rotation of the rotary sealing mechanism 2 , a heating button for controlling the heating plate, and a timing button for controlling the reaction time. The temperature inside the body 1 measured by the temperature sensor can be displayed on the display screen 12 .

[0032] PCR tubes are commonly used consumables in biological experiments. For example, BBSP's PCR tubes are mainly used to provide containers for PCR polymerase chain reaction experiments. They can be used in mutation, sequencing, methylation, molecular cloning, gene expression, genotyping, medicine, forensics and other fields. We directly use this standard product.

[0033] The heating plate is to install the heating wire in the plate, and the heating plate can also be replaced with a heating rod, etc., which mainly plays a heating role. The power source can be a dry battery or a rechargeable battery. The power supply is used to power the system, and the controller can be a PLC.

[0034] The microneedle patch 4 is a microneedle installed on a board. The microneedle patch 4 includes a microneedle 42 and a base plate 43. The microneedle patch 4 is a relatively common assembly in daily life. An L-shaped buckle 44 is provided under the base plate 43, a boss 133 is provided at the opening 131, and a bayonet 134 is provided on the boss. The L-shaped buckle can be snapped into the bayonet 134.

[0035] In this way, the microneedle patch 4 can be placed above the first accommodating space 13 in a relatively fixed position, and the microneedles on the microneedle patch 4 can enter the first accommodating space 13 .

[0036] A disposable container 8 may be provided in the first accommodating space 13. The disposable container 8 includes a cup body 82 and a guide tube 81 provided below the cup body 82. The guide tube 81 is a hose, similar to the water outlet pipe of a washing machine. The front end of the water outlet pipe is relatively hard and can match the lower end of the first guide hole 132. The guide tube 81 is inserted into the first guide hole 132 and is flush with the lower end of the first guide hole 132. In this way, the liquid can be in the disposable container 8 without entering the first guide hole 132. After use, the disposable container 8 can be taken out and directly replaced with a new disposable container 8, so there is no need to clean the first accommodating space 13.

[0037] The principle of the rotating sealing mechanism 2 is that when the second flow guide hole 241 is offset from the first flow guide hole 132, the bottom of the first flow guide hole 132 is blocked, and the liquid in the first accommodating space 13 cannot flow into the PCR tube 51; when the second flow guide hole 241 corresponds to the first flow guide hole 132, the liquid in the first accommodating space 13 can flow into the PCR tube 51.

[0038] In the preferred embodiment, the rotating sealing mechanism 2 includes a mounting plate 21, a DC servo motor 22 is provided below the mounting plate 21, a rotating disk 23 is connected to the extended shaft of the DC servo motor 22, positioning grooves 231 are evenly distributed on the rotating disk 23, the PCR tube 51 is placed in the positioning groove 231, the sealing disk 24 is coaxially installed above the rotating disk 23, and a clamping mechanism 3 is provided at the center position of the sealing disk 24 and the rotating disk 23. The clamping mechanism 3 can float up and down so as to press the sealing disk 24 tightly below the first guide hole 132.

[0039] In this way, under the action of the clamping mechanism 3, the sealing disk 24 is subjected to an upward pressing force, so that the sealing disk 24 is tightly pressed against the bottom of the first guide hole 132, thereby improving the tight fit between the sealing disk 24 and the bottom of the first guide hole 132, thereby preventing leakage.

[0040] Because it is a motor-controlled rotary sealing mechanism, when the flow rate of the solution needs to be quantitatively controlled, for example, 2-5 microliters of TE eluent is transferred to 51, it is only necessary to control the alignment time of the second guide hole 241 and the first guide hole 132. The solution flows downward under the action of gravity. The longer the flow time, the more solution flows. When the flow rate needs to be controlled, it is only necessary to stagger the second guide hole 241 and the first guide hole 132 at a fixed time, thereby achieving the effect of precise control of the flow rate.

[0041] In the preferred embodiment, the clamping mechanism 3 includes a fixed seat 31 connected to the rotating disk 23 and a movable rod 32 fixed on the sealing disk 24. A socket 312 with a first boss 311 is provided in the fixed seat 31, and a fourth groove 321 matching the first boss 311 is provided on the movable rod 32. The movable rod 32 can be inserted into the socket 312, and a spring 33 is also provided between the movable rod 32 and the fixed seat 31.

[0042] Because of the matching first boss 311 and fourth groove 321, the rotating disk 23 and the sealing disk 24 can rotate synchronously. When the clamping mechanism 3 is installed in the body 1, the spring 33 is in a compressed state, so that the sealing disk 24 is subjected to an upward clamping force.

[0043] In a preferred embodiment, a first groove 243 is provided on the outer ring of the sealing disk 24 that contacts the bottom of the first guide hole 132, a second groove 242 is provided around the second guide hole 241, sealing rings are provided in the first groove 243 and the second groove 242, and a protruding mouth 244 is also provided below the second guide hole 241.

[0044] The sealing ring can further improve the fit between the sealing disk 24 and the lower portion of the first guide hole 132 .

[0045] In the preferred embodiment, a circular third groove 16 is provided below the main body 1, a fourth boss 19 is provided on the third groove 16, a notch 191 is provided on the fourth boss 19, the position of the notch 191 corresponds to the position of the buckle 44, a magnet 17 is provided inside, and an iron plate 52 is provided above the microneedle patch 4.

[0046] The magnet 17 can be glued in the third groove 16, and the iron plate 52 can be glued on the top of the microneedle patch 4. In this way, the microneedle patch 4 can be fixed on the magnet 17, and the microneedle patch 4 can be taken out through the notch 191 without touching the microneedles on the microneedle patch 4.

[0047] In a preferred solution, a third accommodating space 15 is further provided in the main body 1 , and a color sensor is provided in the third accommodating space 15 . The color sensor is electrically connected to the controller, and the color sensor is used to detect the color of the liquid in the PCR tube 51 .

[0048] The controller also displays the detection value of the color sensor on the display screen 12. The color sensor 14 is used to detect the color of the solution in the PCR tube 3 after the chemical reaction.

[0049] The color sensor 14 may be a KEYENCE color sensor.

[0050] In a preferred embodiment, a cap 6 for covering the microneedle patch 4 is provided below the body 1, and a cover plate 7 for covering the opening 131 is provided above the body 1. The cap 6 may be provided with an internal thread, and the body 1 may be provided with an external thread below, and the cap 6 is connected by threads.

[0051] Embodiment 2:

[0052] Based on Example 1, further description is given:

[0053] In a preferred embodiment, a fourth accommodating space 61 is provided in the cap 6, a third flow guide hole 62 is provided on the side wall of the cap 6, a through hole 63 is provided below the third flow guide hole 62, and the through hole 63 is used to connect the third flow guide hole 62 and the fourth accommodating space 61. A fourth flow guide hole 18 is also provided on the side wall of the main body 1, and the fourth flow guide hole 18 corresponds to the third flow guide hole 62.

[0054] In this way, the third guide hole 62, the fourth accommodating space 61 and the fourth guide hole 18 are connected, and the liquid in the fourth accommodating space 61 can be introduced or absorbed by the injection guide.

[0055] A second boss 71 is provided on the cover plate 7 , and the second boss 71 can be inserted into the fourth flow guide hole 18 , so that when the fourth flow guide hole 18 is not used, the cover plate 7 can be used to cover the hole.

[0056] In a preferred solution, a third boss 111 is provided above the transparent door 11 , and a corresponding hole is provided on the body 1 , and the third boss 111 can be inserted into the hole of the body 1 .

[0057] In this way, the transparent door 11 can rotate around the third boss 111 to open or close the transparent door 11. When the transparent door 11 is opened, the PCR tube 51 can be placed in the positioning groove 231 of the rotating disk 23.

[0058] A detection method for a plant disease detection pen comprises the following steps:

[0059] Step 1: Use the four ends of the microneedle patch on the detection pen to press and insert into the plant leaf for 10 seconds;

[0060] Step 2: Remove the microneedle patch 4 from the detection pen and soak it in the TE solution in the detection pen;

[0061] Step 3: Place the test pen vertically on a vortex shaker, then place it in a centrifuge tube and centrifuge it slightly to take out the microneedle patch;

[0062] Step 4: Place the PCR tube 51 with the color-developing LAMP in the detection pen, introduce the TE solution in the first accommodation space 13 into the PCR tube 51, and shake the detection pen slightly;

[0063] Step 5: Place the detection pen into the centrifuge tube and centrifuge it. After centrifugation, start the reaction module. After 30 minutes, detect the color in the PCR tube 51 and feed it back to the display screen to directly determine whether there are pathogens in the reaction tube.

[0064] The rotary sealing mechanism 2 rotates the PCR tube 51 to below the color sensor, and the color sensor detects and feeds back the color of the solution in the PCR tube.

[0065] The disease degree of the plant can be checked according to the final color reaction. During the whole reaction process, the controller controls the depyrogenic plate and adjusts the heating plate heating according to the temperature sensor to make the equipment stay at the best reaction temperature.

[0066] The vortex oscillator, centrifuge tube and PCR tube 51 are commonly used instruments and equipment for chemical reactions. According to the previously set color and disease correspondence table, the controller can automatically determine the disease situation of the plant and feed back to the display screen.

[0067] In the above steps, the microneedle patch 4 can also be placed in the first receiving space 13 without being taken out. After the microneedle patch 4 is inserted into the plant leaf for 10 seconds, the cap 6 is covered, and the third guide hole 62 on the cap 6 corresponds to the fourth guide hole 18. Then, the TE solution is injected through the fourth guide hole 18, and the TE solution enters the fourth receiving space 61, thereby dissolving the information on the microneedle patch 4 into the solution, and then the solution is extracted through the fourth guide hole 18 and injected into the first receiving space 13. The main purpose of doing this is to prevent the microneedle patch 4 from being mixed with impurities when taking the microneedle patch 4.

[0068] TE buffer is made of Tris and EDTA. It is mainly used to dissolve nucleic acids and can stably store DNA and RNA.

[0069] The above embodiments are only preferred technical solutions of the present invention and should not be regarded as limiting the present invention. The protection scope of the present invention shall be the technical solutions recorded in the claims, including equivalent replacement solutions of the technical features in the technical solutions recorded in the claims. That is, equivalent replacement improvements within this scope are also within the protection scope of the present invention.

Claims

1. A plant disease on-site detection pen, characterized by: The invention comprises a body (1), a transparent door (11) and a display screen (12) are provided on the outer surface of the body (1), a first accommodating space (13) is provided above the body (1), an opening (131) is provided above the first accommodating space (13), a first flow guide hole (132) is provided below the first accommodating space (13), a second accommodating space (14) for placing a PCR tube (51) is provided below the first flow guide hole (132), a positioning groove (231) is provided in the second accommodating space (14), the PCR tube (51) is placed in the positioning groove (231), the second accommodating space (14) corresponds to the transparent door (11), and a detachable microneedle patch (4) is provided below the body (1).

2. A plant disease on-site detection pen according to claim 1, characterized in that: A rotary sealing mechanism (2) is also provided in the second accommodating space (14). The rotary sealing mechanism (2) comprises a sealing disk (24). The sealing disk (24) is closely located below the first flow guide hole (132). A second flow guide hole (241) is provided at a position of the sealing disk (24) corresponding to the first flow guide hole (132). When the second flow guide hole (241) corresponds to the first flow guide hole (132), liquid in the first accommodating space (13) can flow into the PCR tube (51). The rotary sealing mechanism (2) comprises a mounting A plate (21) is provided below the mounting plate (21), a DC servo motor (22) is provided, a rotating disk (23) is connected to the extended shaft of the DC servo motor (22), positioning grooves (231) are evenly distributed on the rotating disk (23), a sealing disk (24) is coaxially installed above the rotating disk (23), a pressing mechanism (3) is provided at the center of the sealing disk (24) and the rotating disk (23), and the pressing mechanism (3) can float up and down so as to press the sealing disk (24) tightly below the first guide hole (132).

3. A plant disease on-site detection pen according to claim 2, characterized in that: The clamping mechanism (3) comprises a fixed seat (31) connected to the rotating disk (23) and a moving rod (32) fixed to the sealing disk (24); a socket (312) with a first boss (311) is provided in the fixed seat (31); a fourth groove (321) matching the first boss (311) is provided on the moving rod (32); the moving rod (32) can be inserted into the socket (312); and a spring (33) is provided between the moving rod (32) and the fixed seat (31).

4. A plant disease on-site detection pen according to claim 3, characterized in that: A first groove (243) is provided on the outer ring of a surface of the sealing disk (24) in contact with the lower side of the first guide hole (132), a second groove (242) is provided around the second guide hole (241), and sealing rings are provided in the first groove (243) and the second groove (242).

5. The plant disease on-site detection pen according to claim 2, characterized in that: A circular third groove (16) is also provided below the main body (1), a magnet (17) is provided in the third groove (16), and an iron plate (52) is provided above the microneedle patch (4).

6. A plant disease on-site detection pen according to claim 5, characterized in that: A third accommodating space (15) is also provided in the body (1). A color sensor is provided in the third accommodating space (15). The color sensor is electrically connected to the controller. The color sensor is used to detect the color of the liquid in the PCR tube (51).

7. A plant disease on-site detection pen according to claim 6, characterized in that: A cover cap (6) for covering the microneedle patch (4) is also provided below the main body (1), and a cover plate (7) for covering the opening (131) is provided above the main body (1).

8. A plant disease on-site detection pen according to claim 7, characterized in that: A fourth accommodating space (61) is provided in the cover cap (6), a third flow guide hole (62) is provided on the side wall of the cover cap (6), a through hole (63) is provided below the third flow guide hole (62), the through hole (63) is used to connect the third flow guide hole (62) and the fourth accommodating space (61), a fourth flow guide hole (18) is further provided on the side wall of the body (1), the fourth flow guide hole (18) corresponds to the third flow guide hole (62), and a second boss (71) is provided on the cover plate (7), the second boss (71) can be inserted into the fourth flow guide hole (18).

9. The plant disease on-site detection pen according to claim 1, characterized in that: A third boss (111) is provided above the transparent door (11), and a corresponding hole is provided on the body (1), and the third boss (111) can be inserted into the hole of the body (1).

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