Patch clamp perfusion device

Abstract

The utility model discloses a patch clamp perfusion device, which comprises a control module, telescopic electric push rods and a fixed support, the control module comprises a multi-loop time controller and a continuously variable transmission, the fixed support simultaneously fixes a plurality of injectors, one telescopic electric push rod is arranged above a push rod of each injector, and the telescopic electric push rods are connected with the fixed support. A needle cylinder head of each injector corresponds to a needle cylinder head flow guide pipe, each needle cylinder head flow guide pipe is connected with a pipeline, and all the pipelines are converged to a needle head; the telescopic electric push rods are electrically connected with the control module, and under the control of the multi-loop time controller and the continuously variable transmission, the telescopic electric push rods mechanically compress the injector push rods, so that medicine in the injector needle cylinder reaches the needle head through a pipeline. According to the device, regular and constant-speed perfusion administration can be realized; the whole injector can be replaced, a pipeline is convenient to clean, and cross contamination is avoided; meanwhile, the whole device is high in integration degree, small in size, small in occupied space, simple in structure, short in pipeline and free of medicine waste.

Description

Technical Field

[0001] The utility model relates to the technical field of electrophysiological patch clamp, and particularly relates to an electrophysiological patch clamp perfusion device. Background Art

[0002] The electrophysiological patch clamp is a device used for measuring the cell membrane surface. It can confirm the existence of ion channels. Perfusion operation is required during the measurement of the cell membrane. Therefore, strict requirements are imposed on the electrophysiological patch clamp perfusion device. There are still some deficiencies in the existing electrophysiological patch clamp perfusion devices, and the following problems exist during use:

[0003] 1. During use, the perfusion speed and perfusion time cannot be controlled according to requirements, resulting in inconvenient use;

[0004] 2. During use, the pipeline is too long, which not only wastes more reagents, but also requires a longer time to flush the pipeline;

[0005] 3. During use, it is inconvenient to change drugs, and it is easy to cause cross-contamination when different reagents are used in one passage. Content of the Utility Model

[0006] Aiming at the above problems existing in the prior art, the purpose of the utility model is to provide an electrophysiological patch clamp perfusion device capable of perfusing and administering drugs at a fixed time and a fixed speed, so as to solve the problems that the traditional device cannot control the perfusion speed and perfusion time, and is prone to cause cross-contamination and drug waste.

[0007] The technical solution of the utility model is as follows:

[0008] A patch clamp perfusion device includes a control module, a telescopic electric push rod and a fixed bracket. Among them, the control module includes a multi-loop time controller and a stepless speed changer; the fixed bracket fixes multiple syringes at the same time. A telescopic electric push rod is arranged above the push rod of each syringe, and a syringe head guide pipe corresponds to the syringe head of each syringe. Each syringe head guide pipe is connected to a pipeline, and all pipelines converge to a needle head; the telescopic electric push rod is electrically connected to the control module. The start time of each telescopic electric push rod is controlled by the multi-loop time controller, and the propulsion speed of each telescopic electric push rod is controlled by the stepless speed changer. The telescopic electric push rod mechanically compresses the syringe push rod, so that the drug in the syringe barrel reaches the needle head through the pipeline, realizing the perfusion or drug addition function.

[0009] Further, the stepless speed changer in the control module controls the propulsion speed of the telescopic electric push rod by controlling the motor speed in the telescopic electric push rod, so as to regulate the drug outflow speed in the syringe.

[0010] Further, in the above patch clamp perfusion device, a plurality of parallel perfusion channels are provided on the fixed bracket. Each perfusion channel successively includes a telescopic electric push rod, a syringe, a syringe head guide pipe, and a pipeline from top to bottom. After the pipelines are fixed by pipeline fixing seats, they are collectively connected to a needle head.

[0011] The syringe consists of a syringe barrel and a syringe push rod that matches it. In some embodiments of the present invention, a telescopic electric push rod, a syringe limit baffle, a syringe barrel fixator, a syringe head guide pipe, and a pipeline fixing seat are successively arranged on the fixed bracket from top to bottom. The telescopic electric push rod is aligned with the push rod of the syringe. The syringe barrel of the syringe is fixed on the syringe barrel fixator. The syringe limit baffle catches the protruding edge of the upper end face of the syringe barrel. The syringe head is aligned with the syringe head guide pipe, and the syringe head guide pipe is connected to the pipeline. The pipeline fixing seat is provided with card slots corresponding to the number of pipelines. After fixing the pipelines, they are collectively connected to the needle head.

[0012] Further, the syringe barrel fixator consists of two arc-shaped elastic pieces and a fixing bolt. The syringe barrel of the syringe is clamped between the two arc-shaped elastic pieces. A screw hole is provided on one of the arc-shaped elastic pieces, and the syringe barrel is fixed by the fixing bolt.

[0013] In some embodiments of the present invention, the fixed bracket is an inclined plane that forms a certain angle α with the horizontal plane, where 45° ≤ α ≤ 90°. The telescopic electric push rod, the syringe limit baffle, the syringe barrel fixator, the syringe head guide pipe, and the pipeline fixing seat are all fixed on this inclined plane.

[0014] During use, after the syringe draws the drug, it is fixed on the bracket. The control module is turned on. The flow rate is adjusted through the stepless speed changer. The telescopic electric push rod is controlled by the multi-loop time controller. The telescopic electric push rod mechanically compresses the syringe push rod. The drug in the syringe barrel reaches the needle head through the pipeline, realizing the perfusion / drug addition function. At the same time, the multi-loop time controller can arbitrarily control the opening and closing of a certain channel and has the function of timed drug addition.

[0015] The patch clamp perfusion device of the present invention has the following beneficial effects compared with the prior art: This device can realize timed and constant-speed perfusion drug administration; the syringe can be replaced as a whole, and the pipeline is convenient to clean, avoiding the problem of cross-contamination; the whole device has a high degree of integration, is small in size, and does not occupy space; at the same time, the patch clamp perfusion device has a simple structure and short pipelines, and will not cause the problem of drug waste. Description of the Drawings

[0016] Figure 1 is the plane structure diagram of the patch clamp perfusion device of the present invention.

[0017] Figure 2 is the schematic structural diagram of the fixed bracket of the patch clamp perfusion device of the present invention.

[0018] Figure 3 It is a schematic structural diagram of fixing a syringe to a bracket in the patch clamp perfusion device of the present utility model.

[0019] In the figure: 1. Telescopic electric push rod; 2. Syringe push rod; 3. Syringe limit baffle; 4. Fixing bolt; 5. Syringe barrel fixer; 6. Syringe barrel; 7. Syringe head guide tube; 8. Pipeline; 9. Pipeline fixing seat; 10. Multi-loop time controller; 11. Needle head; 12. Power switch; 13. Stepless speed changer; 14. Bracket. Specific embodiments

[0020] In order to make the technical problems to be solved, the technical solutions adopted and the beneficial effects obtained by the present utility model clearer and more understandable, the present utility model will be described in detail below with reference to the accompanying drawings through embodiments. It should be understood that the embodiments described herein are only used to explain the present utility model and are not used to limit the scope of the present utility model.

[0021] As Figure 1 shown, the patch clamp perfusion device includes a plurality of perfusion / drug addition channels arranged in parallel. Each perfusion / drug addition channel is sequentially provided with a telescopic electric push rod 1, a syringe, a syringe head guide tube 9, and a pipeline 8 from top to bottom. Each pipeline 8 converges and connects to a needle head 11 at the pipeline fixing seat 9. Among them, the syringe is composed of a syringe barrel 6 and a syringe push rod 2 matching therewith; the telescopic electric push rod 1, the syringe limit baffle 3, the syringe barrel fixer 5, the syringe head guide tube 7, and the pipeline fixing seat 9 are all fixedly arranged on the bracket. As Figure 2 shown, the bracket is an inclined plane at a certain angle α with the horizontal plane, 45° ≤ α ≤ 90°. Each syringe barrel fixer 5 includes two arc-shaped elastic pieces for clamping the syringe barrel 6 and a fixing bolt 4, and a screw hole is provided on one of the arc-shaped elastic pieces. A syringe limit baffle 3 is arranged above the syringe barrel fixer 5 for clamping the protruding edge of the upper end face of the syringe barrel 6; a syringe head guide tube 7 is fixed below the syringe barrel fixer 5 for connecting the injection head at the lower end face of the syringe barrel 6. After the syringe extracts the drug, it is fixed on the bracket 14 through the syringe limit baffle 3, the fixing bolt 4, and the syringe barrel fixer 5. The upper part of the syringe push rod 2 is directly opposite to the telescopic electric push rod 1, and the syringe needle head corresponds to the syringe head guide tube 7; the lower end of the syringe head guide tube 7 is connected to the pipeline 8, and each pipeline 8 is fixed in the card slot of the pipeline fixing seat 9 and then converges and connects to the needle head 11. Each telescopic electric push rod 1 is electrically connected to the control module, and each telescopic electric push rod 1 is controlled regularly through the multi-loop time controller 10 of the control module; the stepless speed changer 13 of the control module controls the motor speed in the telescopic electric push rod 1, thereby controlling the pushing speed of the telescopic electric push rod and realizing the regulation of the flow rate.

[0022] In use, turn on the power switch 12 of the control module, adjust the flow rate through the stepless speed variator 13, control the telescopic electric push rod 1 through the multi-loop time controller 10. The telescopic electric push rod 1 mechanically compresses the syringe push rod 2, and the drug in the syringe barrel 6 reaches the needle 11 through the pipeline 8, realizing the perfusion / drug addition function. At the same time, the multi-loop time controller 10 can arbitrarily control the opening and closing of a certain channel and has the function of timed drug addition.

[0023] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A patch clamp perfusion device, characterized in that: It includes a control module, a telescopic electric push rod and a fixed bracket, wherein the control module includes a multi-circuit time controller and a continuously variable transmission; the fixed bracket fixes multiple syringes at the same time, a telescopic electric push rod is arranged above the push rod of each syringe, the syringe head of each syringe corresponds to a syringe head guide tube, each syringe head guide tube is connected to a pipeline, and all pipelines are gathered into a needle; the telescopic electric push rod is electrically connected to the control module, the starting time of each telescopic electric push rod is controlled by the multi-circuit time controller, the propulsion speed of each telescopic electric push rod is controlled by the continuously variable transmission, and the telescopic electric push rod mechanically compresses the syringe push rod so that the medicine in the syringe barrel reaches the needle through the pipeline.

2. The patch clamp perfusion device according to claim 1, characterized in that: A plurality of parallel perfusion channels are arranged on the fixed bracket, and each perfusion channel is provided with a telescopic electric push rod, a syringe, a syringe head guide tube, and a pipeline from top to bottom. The pipelines are fixed by a pipeline fixing seat and then converged to connect the needle.

3. The patch clamp perfusion device according to claim 2, characterized in that: A telescopic electric push rod, a syringe limit baffle, a syringe holder, a syringe head guide tube and a pipeline fixing seat are arranged in sequence from top to bottom on the fixed bracket, the telescopic electric push rod is aligned with the push rod of the syringe, the syringe barrel of the syringe is fixed on the syringe holder, the syringe limit baffle clamps the protruding edge of the upper end surface of the syringe, the syringe head is aligned with the syringe head guide tube, and the syringe head guide tube is connected to the pipeline; the pipeline fixing seat is provided with slots corresponding to the number of pipelines, and the pipelines are fixed and then gathered together to connect the needles.

4. The patch clamp perfusion device according to claim 3, characterized in that: The syringe holder is composed of two arc-shaped elastic sheets and a fixing bolt. The syringe of the syringe is clamped between the two arc-shaped elastic sheets. A screw hole is provided on one of the arc-shaped elastic sheets, and the syringe is fixed by the fixing bolt.

5. The patch clamp perfusion device according to claim 3, characterized in that: The fixed bracket is an inclined plane which forms a certain angle α with the horizontal plane, 45°≤α≤90°, and the telescopic electric push rod, syringe limit baffle, syringe holder, syringe head guide tube and pipeline fixing seat are fixed on the inclined plane.

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