Telaprevir intermediate fermentation liquor separation and purification device

Abstract

The utility model is applicable to the technical field of telaprevir intermediate fermentation liquor preparation, and provides a telaprevir intermediate fermentation liquor separation and purification device which comprises a raw material tank, a centrifugal device, a ceramic membrane device and an ultrafiltration membrane device, a second channel is communicated between the centrifugal device and the ceramic membrane device; the ceramic membrane device and the ultrafiltration membrane device are communicated with a third channel; a ceramic membrane is arranged in the ceramic membrane device, and an ultrafiltration membrane is arranged in the ultrafiltration membrane device. The utility model provides a telaprevir intermediate fermentation liquor separation and purification device, which reduces the production cycle, improves the efficiency and is safe and reliable. Membrane filtration is adopted, equipment parameters are easy to adjust, and the product content can be kept at a stable level; and compared with the traditional natural sedimentation process, the centrifugal device is adopted, so that the efficiency is greatly improved, and the pollution is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of the preparation of Telaprevir intermediate fermentation broth, and particularly relates to a separation and purification device for Telaprevir intermediate fermentation broth. Background Art

[0002] Telaprevir, English name: Telaprevir, molecular formula C36H35N7O6, molecular weight 679.85. Telaprevir is an effective and selective reversible HCV NS3-4A protease inhibitor, acting on the NS3 protease domain of genotype 1 (H strain) and the NS4A cofactor peptide, and is a new drug for the treatment of hepatitis C.

[0003] Most of the existing devices for preparing Telaprevir intermediate fermentation broth have complex processes and structures; after fermentation, natural sedimentation is adopted, which takes a long time, chemical agents are used during the sedimentation process, and the dosage is large, which is easy to cause environmental pollution. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a separation and purification device for Telaprevir intermediate fermentation broth to solve the problems in the above background art.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: A separation and purification device for Telaprevir intermediate fermentation broth, including a raw material tank, a centrifugal device, a ceramic membrane device and an ultrafiltration membrane device. A first pipeline is connected and communicated between the raw material tank and the centrifugal device, and a second channel is connected and communicated between the centrifugal device and the ceramic membrane device; a third channel is connected and communicated between the ceramic membrane device and the ultrafiltration membrane device; a ceramic membrane is arranged inside the ceramic membrane device, and an ultrafiltration membrane is arranged inside the ultrafiltration membrane device.

[0006] Preferably, the centrifugal device includes a tank body and a bracket. A sleeve is arranged at the top of the bracket. A first annular clamping groove is formed in the side wall of the sleeve. An annular block is arranged on the side wall of the tank body, and the annular block is connected and matched with the first annular clamping groove. A second annular clamping groove is arranged on the inner wall of the top of the tank body, and a top plate is clamped inside the second annular clamping groove. The first pipeline passes through the top plate. A liquid outlet pipe is connected and communicated with the side wall of the tank body. A water storage tank is arranged on the side wall of the bracket below the liquid outlet pipe. The second channel is communicated with the water storage tank. A toothed ring is arranged on the side wall of the top of the tank body. A motor is arranged on one side of the top surface of the sleeve. A gear is arranged at the output end of the motor, and the gear is meshed with the toothed ring.

[0007] Preferably, an annular baffle is arranged on the inner wall of the tank body below the liquid outlet pipe, and the free end of the annular baffle is inclined downward.

[0008] Preferably, the bottom of the tank body is conical, and a discharge pipe is arranged on the bottom surface of the tank body.

[0009] Preferably, the ceramic membrane separation aperture used in the ceramic membrane device is 200 nm.

[0010] The utility model has at least the following beneficial effects:

[0011] (1) The utility model provides a separation and purification device for telaprevir intermediate fermentation broth, which reduces the production cycle, improves efficiency, and is safe and reliable;

[0012] (2) The utility model provides a separation and purification device for telaprevir intermediate fermentation broth, which adopts membrane filtration, is simple in adjusting equipment parameters, and the product content can be kept at a stable level;

[0013] (3) The utility model provides a separation and purification device for telaprevir intermediate fermentation broth, which adopts a centrifugal device, greatly improves efficiency compared with the traditional natural sedimentation process, and reduces pollution. Description of the Drawings

[0014] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0015] Figure 2 It is a schematic diagram of the internal structure of the centrifugal device in the utility model;

[0016] Figure 3 It is a schematic process diagram of the utility model.

[0017] In the reference numerals of the drawings: 1, raw material tank; 2, centrifugal device; 3, ceramic membrane device; 4, ultrafiltration membrane device; 5, first pipeline; 6, water storage tank; 7, second channel; 8, third channel; 9, bracket; 10, sleeve; 11, first annular clamping groove; 12, annular block; 13, toothed ring; 14, gear; 15, motor; 16, second annular clamping groove; 17, top plate; 18, annular baffle. Detailed Embodiments

[0018] Next, the technical solutions in the embodiments of the utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only a part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the utility model.

[0019] Embodiment

[0020] Please refer to Figure 1 、 Figure 2 and Figure 3, the present utility model provides a technical solution: a separation and purification device for telaprevir intermediate fermentation broth, which includes a raw material tank 1, a centrifugation device 2, a ceramic membrane device 3, and an ultrafiltration membrane device 4. A first pipeline 5 is connected in communication between the raw material tank 1 and the centrifugation device 2, and a second channel 7 is connected in communication between the centrifugation device 2 and the ceramic membrane device 3; a third channel 8 is connected in communication between the ceramic membrane device 3 and the ultrafiltration membrane device 4; a ceramic membrane is provided inside the ceramic membrane device 3, and an ultrafiltration membrane is provided inside the ultrafiltration membrane device 4. Specifically, the spiral wound ultrafiltration membrane used in the ultrafiltration membrane device 4 has a molecular weight cut-off of 2500 Daltons.

[0021] In this embodiment, the telaprevir intermediate fermentation broth in the raw material tank 1 enters the centrifugation device 2 through the first pipeline 5. After centrifugation by the centrifugation device 2, the supernatant enters the ceramic membrane device 3 through the second pipeline 7;

[0022] The ceramic membrane device 3 filters the centrifuged supernatant with a 200-nm ceramic membrane device at a temperature of 40 °C and a pressure of 0.3 MPa; the dialysate obtained after the treatment by the ceramic membrane device 3 enters the ultrafiltration membrane device 4 through the third pipeline 8.

[0023] Furthermore, the centrifugation device 2 includes a tank body and a bracket 9. A sleeve 10 is provided at the top of the bracket 9. Specifically, the sleeve 10 is fixedly connected to the bracket 9. A first annular clamping groove 11 is formed on the side wall of the sleeve 10. An annular block 12 is provided on the side wall of the tank body. Specifically, the annular block 12 is fixedly connected to the tank body. The annular block 12 is in fit connection with the first annular clamping groove 11. Specifically, the annular block 12 slides inside the first annular clamping groove 11. A second annular clamping groove 16 is provided on the inner wall of the top of the tank body, and a top plate 17 is clamped inside the second annular clamping groove 16. Specifically, the top plate 17 slides inside the second annular clamping groove 16. The first pipeline 5 passes through the top plate 17. Specifically, the first pipeline 5 is fixedly connected to the top plate 17. A liquid outlet pipe is communicated with the side wall of the tank body. A water storage tank 6 is provided on the side wall of the bracket 9 below the liquid outlet pipe. Specifically, the water storage tank 6 is fixedly connected to the bracket 9. The second channel 7 is communicated with the water storage tank 6. A toothed ring 13 is provided on the side wall of the top of the tank body. Specifically, the toothed ring 13 is fixedly connected to the tank body. A motor 15 is provided on one side of the top surface of the sleeve 10. Specifically, the motor 15 is fixedly connected to the top surface of the sleeve 10. A gear 14 is provided at the output end of the motor 15. Specifically, the gear 14 is fixedly connected to the output end of the motor 15. The gear 14 meshes with the toothed ring 13

[0024] In this embodiment, the telaprevir intermediate fermentation broth in the raw material tank 1 enters the tank body of the centrifugal device 2 through the first pipeline 5. The motor 15 is started, and the motor 15 drives the gear 14 to rotate, driving the toothed ring 13 to rotate, and then driving the tank body to rotate. During the rotation of the tank body, the annular block 12 rotates along the first annular card slot 11. The rotation speed of the centrifugal device 2 is 3000 - 5000 rpm. Solid impurities accumulate at the bottom of the tank body. When the centrifugal device 2 stops rotating, the liquid outlet pipe just stops above the reservoir 6, and the supernatant is released into the water storage tank 6 through the liquid outlet pipe.

[0025] Furthermore, an annular baffle 18 is provided on the inner wall of the tank body below the liquid outlet pipe. Specifically, the annular baffle 18 is fixedly connected to the inner wall of the tank body, and the free end of the annular baffle 18 is inclined downward.

[0026] In this embodiment, the annular baffle 18 is provided to prevent a large amount of impurities accumulated at the bottom of the tank body from being carried out when releasing the supernatant. The annular baffle 18 has a certain blocking effect, and the free end of the annular baffle 18 is inclined downward, which can guide the solid impurities to the bottom of the tank body.

[0027] Furthermore, the bottom of the tank body is conically arranged, and a discharge pipe is provided on the bottom surface of the tank body.

[0028] In this embodiment, the discharge pipe on the bottom surface of the tank body facilitates the discharge of impurities.

[0029] Furthermore, the ceramic membrane separation aperture adopted by the ceramic membrane device 3 is 200 nm.

[0030] In this embodiment, the ceramic membrane separation aperture of 200 nm is convenient for filtration and dialysis.

[0031] The working principle and usage process of the present utility model: After the present utility model is installed, it works according to the above embodiment until all working steps are completed.

[0032] The test results of the device in this embodiment are as follows:

[0033] 1) Ceramic membrane filtration

[0034] The supernatant after centrifugation is filtered with a 200 nm ceramic membrane at a temperature of 40 °C and a pressure of 0.3 MPa, and 3 experiments are carried out before and after.

[0035]

[0036]

[0037] Analyzing the data of 3 batches shows that the ceramic membrane has good filtration effect, high concentration multiple, high product yield and other characteristics when filtering the supernatant.

[0038] 2) Ultrafiltration membrane device

[0039] The supernatant is filtered through a ceramic membrane to obtain the dialysate. Since the ceramic membrane has no interception effect on high molecular proteins and pigments, which will affect the final purity of the subsequent product, an ultrafiltration membrane is used for removal.

[0040]

[0041] Analyzing the above three batches of data, it can be obtained that the protein removal rate of the ceramic membrane dialysate can reach over 90%, the light transmittance is above 96% for all, and the product yield is about 98% for all, which is beneficial to the purification of the subsequent process.

[0042] The above shows and describes the basic principles, main features and advantages of the present invention. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention, and any reference signs in the claims should not be regarded as limiting the claims involved.

[0043] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A telaprevir intermediate fermentation broth separation and purification device, characterized in that: The invention comprises a raw material tank (1), a centrifugal device (2), a ceramic membrane device (3) and an ultrafiltration membrane device (4); a first pipeline (5) is provided between the raw material tank (1) and the centrifugal device (2); a second channel (7) is provided between the centrifugal device (2) and the ceramic membrane device (3); a third channel (8) is provided between the ceramic membrane device (3) and the ultrafiltration membrane device (4); a ceramic membrane is provided inside the ceramic membrane device (3), and an ultrafiltration membrane is provided inside the ultrafiltration membrane device (4).

2. A telaprevir intermediate fermentation broth separation and purification device according to claim 1, characterized in that: The centrifugal device (2) comprises a tank body and a bracket (9). A sleeve (10) is provided on the top of the bracket (9). A first annular groove (11) is provided on the side wall of the sleeve (10). An annular block (12) is provided on the side wall of the tank body. The annular block (12) is matched and connected with the first annular groove (11). A second annular groove (16) is provided on the top inner wall of the tank body. A top plate (17) is connected inside the second annular groove (16). The first pipeline (5) passes through the top plate (17). A liquid outlet pipe is provided on the side wall of the tank body. A water storage tank (6) is provided on the side wall of the bracket (9) below the liquid outlet pipe. The second channel (7) is connected with the water storage tank (6). A gear ring (13) is provided on the top side wall of the tank body. A motor (15) is provided on one side of the top surface of the sleeve (10). A gear (14) is provided at the output end of the motor (15). The gear (14) is meshed with the gear ring (13).

3. A telaprevir intermediate fermentation broth separation and purification device according to claim 2, characterized in that: An annular baffle (18) is provided on the inner wall of the tank body below the liquid outlet pipe, and the free end of the annular baffle (18) is arranged to be inclined downward.

4. A telaprevir intermediate fermentation broth separation and purification device according to claim 3, characterized in that: The bottom of the tank body is conical, and a discharge pipe is arranged on the bottom surface of the tank body.

5. The device for separating and purifying telaprevir intermediate fermentation broth according to claim 1, characterized in that: The ceramic membrane separation pore size used in the ceramic membrane device (3) is 200 nm.

Images