Auxiliary device for accurately cutting gel
By designing an accurate cutting auxiliary device including a cassette, glass plate, press plate, light source, rotating shaft, light shielding roll and sliding components, the problem of inaccurate gel cutting in western blotting experiments was solved, and high-precision gel cutting was achieved, reducing experimental errors.
Patent Information
- Application Number
- CN202421664178.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-15
- Publication Date
- 2025-05-30
- Estimated Expiration
- 2034-07-15
AI Technical Summary
In western blot experiments, due to inaccurate measurements during the electrophoretic gel cleavage, secondary processing or target proteins are often cleaved, which affects the experimental process and results.
An auxiliary device for accurately cutting gel is designed, including a cassette, glass plate, press plate, light source, rotary shaft, light shielding roll and sliding components, which are accurately cut through the presentation of light strips to avoid direct measurement errors.
Accurate cutting of the gel is achieved, experimental errors are reduced, the smooth progress of the experiment is ensured, and secondary processing or target protein is cleaved.
Smart Images
Figure CN222926660U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of protein immunoblotting, and particularly relates to an auxiliary device for precisely cutting gels. Background Art
[0002] The protein immunoblotting experiment is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. This method is a new immunochemical technology developed on the basis of gel electrophoresis and solid-phase immunoassay techniques. Due to the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassay in immunoblotting, it has now become a conventional technique for protein analysis.
[0003] In the protein immunoblotting experiment, during the process of cutting the electrophoresis gel, there are often situations where secondary processing occurs due to inaccurate measurement or the target protein is cut off, thus affecting the experimental process and results. Summary of the Utility Model
[0004] In order to solve the problem that in the existing protein immunoblotting experiment, during the process of cutting the electrophoresis gel, there are often situations where secondary processing occurs due to inaccurate measurement or the target protein is cut off, the utility model provides an auxiliary device for precisely cutting gels.
[0005] The technical solution adopted by the utility model to solve the technical problem is as follows:
[0006] An auxiliary device for precisely cutting gels of the utility model mainly includes:
[0007] An auxiliary device for precisely cutting gels, characterized by including:
[0008] A light-tight box;
[0009] A left rotating shaft and a right rotating shaft installed on the front and rear box walls of the light-tight box;
[0010] A left light-shielding cloth reel installed on the left rotating shaft;
[0011] A left cross bar installed at the edge of the light-shielding cloth at the front end of the left light-shielding cloth reel;
[0012] A right light-shielding cloth reel installed on the right rotating shaft;
[0013] A right cross bar installed at the edge of the light-shielding cloth at the front end of the right light-shielding cloth reel;
[0014] A rear rotating shaft and a front rotating shaft installed on the left and right box walls of the light-tight box;
[0015] A rear light-shielding cloth reel installed on the rear rotating shaft;
[0016] A rear cross bar installed at the edge of the light-shielding cloth at the front end of the rear light-shielding cloth reel;
[0017] The front light-shielding cloth reel installed on the front rotating shaft;
[0018] The front cross bar installed at the edge of the light-shielding cloth at the front end of the front light-shielding cloth reel;
[0019] The light source installed on the rear inner box wall of the cassette;
[0020] The glass plate installed at the upper end of the cassette;
[0021] The pressing plate installed above the glass plate;
[0022] The fixing groove provided on the pressing plate;
[0023] The horizontal movement groove provided on the pressing plate, the horizontal movement groove is located on the front side of the fixing groove and penetrates through the pressing plate and the glass plate;
[0024] The vertical movement groove provided on the pressing plate, the horizontal movement groove is located on the left side of the fixing groove and penetrates through the pressing plate and the glass plate;
[0025] The first sliding assembly and the second sliding assembly installed in the horizontal movement groove, the first sliding assembly is connected to the left cross bar, and the second sliding assembly is connected to the right cross bar;
[0026] The third sliding assembly and the fourth sliding assembly installed in the vertical movement groove, the third sliding assembly is connected to the rear cross bar, and the fourth sliding assembly is connected to the front cross bar.
[0027] Furthermore, the present utility model further includes that the structural dimensions of the first sliding assembly, the second sliding assembly, the third sliding assembly and the fourth sliding assembly are all the same.
[0028] Furthermore, the present utility model further includes that the first sliding assembly is composed of a connecting plate, a connecting rod, a slider, a slider bottom plate, a slider top plate, a slider handle and a pointer. The slider is arranged in the horizontal movement groove. A slider top plate is installed above the slider, and a pointer is provided on the slider top plate. The edge of the slider top plate is located above the surface of the pressing plate. A slider bottom plate is installed below the slider, and the edge of the slider bottom plate is located below the glass plate. The slider, the slider bottom plate and the slider top plate are in an I-shaped structure and slide in the horizontal movement groove.
[0029] Furthermore, the present utility model further includes that the connecting plate on the first sliding assembly is connected to the left cross bar, the connecting plate on the second sliding assembly is connected to the right cross bar, the connecting plate on the third sliding assembly is connected to the rear cross bar, and the connecting plate on the fourth sliding assembly is connected to the front cross bar.
[0030] Furthermore, the present utility model further includes that the light source is a long straight strip-shaped light-emitting body with uniform brightness, and the light source is horizontally installed on the rear inner box wall of the cassette.
[0031] Furthermore, the utility model further includes that the glass plate is made of rectangular transparent material.
[0032] Furthermore, the utility model further includes that the pressing plate is made of non-transparent material, and the length and width of the pressing plate are the same as those of the glass plate and are attached to the glass plate.
[0033] Furthermore, the utility model further includes that horizontal scale lines are arranged on the edge of the horizontal movement slot near the fixed slot, and vertical scale lines are arranged on the edge of the vertical movement slot near the fixed slot.
[0034] Furthermore, the utility model further includes that the pointers of the first sliding component and the second sliding component point to the horizontal scale lines, and the pointers of the third sliding component and the fourth sliding component point to the vertical scale lines.
[0035] The beneficial effects of the utility model are as follows:
[0036] An auxiliary device for precisely cutting a gel of the utility model mainly includes a dark box, a glass plate, a pressing plate, a light source, a rotating shaft, a light-shielding cloth reel, a cross bar and a sliding component. During the process of cutting an electrophoresis gel in a protein immunoblotting experiment, it is not necessary to remove the gel from the gel-making glass plate, and the gel is cut according to the range of the presented light band, so as to realize the precise cutting of the gel, avoid the situation of inaccurate measurement when cutting the gel, resulting in secondary processing or the target protein being cut off, reduce experimental errors, and thus ensure the smooth progress of the experiment. Description of the Drawings
[0037] Figure 1 It is a three-dimensional perspective schematic diagram of an auxiliary device for precisely cutting a gel of the utility model.
[0038] Figure 2 It is a top view of an auxiliary device for precisely cutting a gel of the utility model.
[0039] Figure 3 It is a three-dimensional structural schematic diagram of the sliding component of an auxiliary device for precisely cutting a gel of the utility model.
[0040] Figure 4 It is a three-dimensional structural schematic diagram of the slider part of the slider component of an auxiliary device for precisely cutting a gel of the utility model.
[0041] In the figure, 1 is a cassette, 2 is a glass plate, 3 is a pressing plate, 4 is a fixing groove, 5 is a horizontal moving groove, 6 is a vertical moving groove, 7 is a horizontal scale line, 8 is a vertical scale line, 9 is a light source, 101 is a rear rotating shaft, 102 is a front rotating shaft, 103 is a left rotating shaft, 104 is a right rotating shaft, 111 is a rear light-shielding cloth reel, 112 is a front light-shielding cloth reel, 113 is a left light-shielding cloth reel, 114 is a right light-shielding cloth reel, 121 is a rear cross bar, 122 is a front cross bar, 123 is a left cross bar, 124 is a right cross bar, 13 is a connecting plate, 14 is a connecting rod, 15 is a slider, 16 is a slider bottom plate, 17 is a slider top plate, 18 is a slider handle, 19 is a pointer. Detailed implementation mode
[0042] The present utility model will be further described in detail below with reference to the accompanying drawings.
[0043] As Figures 1 to 4 shown, an auxiliary device for precisely cutting gel of the present utility model mainly includes the following components:
[0044] Cassette 1, glass plate 2, pressing plate 3, fixing groove 4, horizontal moving groove 5, vertical moving groove 6, horizontal scale line 7, vertical scale line 8, light source, rear rotating shaft 101, front rotating shaft 102, left rotating shaft 103, right rotating shaft 104, rear light-shielding cloth reel 111, front light-shielding cloth reel 112, left light-shielding cloth reel 113, right light-shielding cloth reel 114, rear cross bar 121, front cross bar 122, left cross bar 123, right cross bar 124, connecting plate 13, connecting rod 14, slider 15, slider bottom plate 16, slider top plate 17, slider handle 18, pointer 19, first sliding component A, second sliding component B, third sliding component C, fourth sliding component D.
[0045] The cassette 1 is a cuboid structure with a hollow interior and no upper cover. There are 2 rotating shaft holes provided on each of the front, rear, left, and right box walls of the cassette 1, and the rotating shaft holes on the front and rear box walls correspond to each other, and the rotating shaft holes on the left and right box walls correspond to each other.
[0046] The left rotating shaft 103 and the right rotating shaft 104 pass through the front and rear box walls of the cassette 1 through the rotating shaft holes, and the left rotating shaft 103 and the right rotating shaft 104 are parallel to each other.
[0047] The left light-shielding cloth reel 113 is fixedly installed at the middle position of the left rotating shaft 103, and the left rotating shaft 103 rotates with the rotation of the left light-shielding cloth reel 113.
[0048] The left cross bar 123 is fixedly installed at the edge of the light-shielding cloth at the front end of the left light-shielding cloth reel 113.
[0049] The right light-shielding cloth reel 114 is fixedly installed at the middle position of the right rotating shaft 104, and the right rotating shaft 104 rotates with the rotation of the right light-shielding cloth reel 114.
[0050] A right cross bar 124 is fixedly installed at the edge of the light-shielding cloth at the front end of the right light-shielding cloth reel 114.
[0051] The rear rotating shaft 101 and the front rotating shaft 102 pass through the left and right box walls of the light-tight box 1 through the rotating shaft holes, and the rear rotating shaft 101 and the front rotating shaft 102 are placed in parallel.
[0052] A rear light-shielding cloth reel 111 is fixedly installed at the middle position of the rear rotating shaft 101, and the rear rotating shaft 101 rotates as the rear light-shielding cloth reel 111 rotates.
[0053] A rear cross bar 121 is fixedly installed at the edge of the light-shielding cloth at the front end of the rear light-shielding cloth reel 111.
[0054] A front light-shielding cloth reel 112 is fixedly installed at the middle position of the front rotating shaft 102, and the front rotating shaft 102 rotates as the front light-shielding cloth reel 112 rotates.
[0055] A front cross bar 122 is fixed at the edge of the light-shielding cloth at the front end of the front light-shielding cloth reel 112.
[0056] A light source 9 is horizontally installed on the rear inner box wall of the light-tight box 1, and the light source 9 is a long straight strip-shaped light-emitting body with uniform brightness.
[0057] The glass plate 2 is installed at the upper end of the light-tight box 1. The four edges of the glass plate 2 are connected to the four sides of the upper end of the light-tight box 1, and the glass plate 2 is made of rectangular transparent material.
[0058] The pressing plate 3 is fixedly installed above the glass plate 2. The pressing plate 3 is made of non-transparent material, and the length and width of the pressing plate 3 are the same as those of the glass plate 2 and are in fit with the glass plate 2. A fixing groove 4 is provided at the middle position of the pressing plate 3. The shape of the fixing groove 4 is rectangular, and the internal space of the light-tight box 1 can be seen through the fixing groove 4 and the glass plate 2.
[0059] A horizontal moving groove 5 is provided on the pressing plate 3. The horizontal moving groove 5 is located on the front side of the fixing groove 4 and penetrates through the pressing plate 3 and the glass plate 2.
[0060] A vertical moving groove 6 is provided on the pressing plate 3. The vertical moving groove 6 is located on the left side of the fixing groove 4 and penetrates through the pressing plate 3 and the glass plate 2.
[0061] A horizontal scale line 7 is provided at the edge of the horizontal moving groove 5 close to the fixing groove 4.
[0062] A first sliding assembly A and a second sliding assembly B are installed in the horizontal moving groove 5.
[0063] A vertical scale line 8 is provided at the edge of the vertical moving groove 6 close to the fixing groove 4.
[0064] A third sliding assembly C and a fourth sliding assembly D are installed in the vertical moving groove 6.
[0065] The structural dimensions of the first sliding component A, the second sliding component B, the third sliding component C, and the fourth sliding component D are all the same. Now, the first sliding component A will be taken as an example for illustration.
[0066] The first sliding component A is composed of a connecting plate 13, a connecting rod 14, a slider 15, a slider bottom plate 16, a slider top plate 17, a slider handle 18, and a pointer 19.
[0067] The slider 15 is arranged on the left side inside the horizontal movement groove 5. A slider top plate 17 is installed above the slider 15, and the edge of the slider top plate 17 is located above the surface of the pressure plate 3. A slider bottom plate 16 is installed below the slider 15, and the edge of the slider bottom plate 16 is located below the glass plate 2. The slider 15, the slider bottom plate 16, and the slider top plate 17 are in an I-shaped structure and slide inside the horizontal movement groove 5.
[0068] An installation hole is provided on the slider 15, and the upper end of the connecting rod 14 passes through the slider bottom plate 16 and is fixedly installed in the installation hole of the slider 15.
[0069] An installation hole is provided at the edge of the connecting plate 13, and the lower end of the connecting rod 14 is fixedly installed in the installation hole of the connecting plate 13.
[0070] A pointer 19 is provided on the slider top plate 17.
[0071] A slider handle 18 is fixedly installed on the upper surface of the slider top plate 17, and an operator can control the position of the first sliding component A inside the horizontal movement groove 5 by operating the slider handle 18.
[0072] The connecting plate 13 on the first sliding component A is connected and fixed to the left cross bar 123. The connecting plate 13 has the same width as the left cross bar 123. The tip of the pointer 19 on the first sliding component A is vertically directly above the right end edge of the left cross bar 123.
[0073] The connecting plate 13 on the second sliding component B is connected and fixed to the right cross bar 124. The connecting plate 13 has the same width as the right cross bar 124. The tip of the pointer 19 on the second sliding component B is vertically directly above the left end edge of the right cross bar 124.
[0074] The connecting plate 13 on the third sliding component C is connected and fixed to the rear cross bar 121. The connecting plate 13 has the same width as the rear cross bar 121. The tip of the pointer 19 on the third sliding component C is vertically directly above the front end edge of the rear cross bar 121.
[0075] The connecting plate 13 on the fourth sliding component D is connected and fixed to the front cross bar 122. The connecting plate 13 has the same width as the front cross bar 122. The tip of the pointer 19 on the fourth sliding component D is vertically directly above the rear end edge of the front cross bar 122.
[0076] The pointers 19 of the first sliding component A and the second sliding component B point to the horizontal scale line 7.
[0077] The pointers 19 of the third sliding component C and the fourth sliding component D point to the vertical scale line 8.
[0078] An auxiliary device for precisely cutting gels of the present utility model is mainly for cutting electrophoresis gels in protein immunoblotting experiments.
[0079] The working process of an auxiliary device for precisely cutting gels of the present utility model is as follows:
[0080] During the process of cutting electrophoresis gels in protein immunoblotting experiments, the experimenter places the gel-making glass plate in the fixing groove 4, and drives the four sliding components to move to the target positions respectively by operating the slider handles 18 on the first sliding component A, the second sliding component B, the third sliding component C, and the fourth sliding component D. The pointers 19 of the first sliding component A and the second sliding component B point to the target scale of the horizontal scale line 7, and the scale difference between the two pointers is the width of the target size of the experimental gel. The pointers 19 of the third sliding component C and the fourth sliding component D point to the target scale of the vertical scale line 8, and the scale difference between the two pointers is the length of the target size of the experimental gel. The connecting plate 13 on the first sliding component A drives the left cross bar 123 and the light-shielding cloth to move to the target position, the connecting plate 13 on the second sliding component B drives the right cross bar 124 and the light-shielding cloth to move to the target position, the connecting plate 13 on the third sliding component C drives the rear cross bar 121 and the light-shielding cloth to move to the target position, the connecting plate 13 on the fourth sliding component D drives the front cross bar 122 and the light-shielding cloth to move to the target position, and a closed light-transmitting square area of the target size is formed in the middle of the rear cross bar 121, the front cross bar 122, the left cross bar 123, and the right cross bar 124. The light emitted by the light source 9 passes through the closed light-transmitting square area and irradiates on the gel of the gel-making plate, and a light strip of the target size appears on the gel. The experimenter cuts the gel according to the range of the light strip to achieve precise cutting operation of the gel. After cutting is completed, the experimenter removes the gel-making glass plate. After obtaining the gel in the target area, by rotating the rear rotating shaft 101, the front rotating shaft 102, the left rotating shaft 103, and the right rotating shaft 104, the light-shielding cloth is driven to wind back onto the rear light-shielding cloth reel 111, the front light-shielding cloth reel 112, the left light-shielding cloth reel 113, and the right light-shielding cloth reel 114 to realize the reset of the light-shielding cloth for convenient use in the next time.
[0081] An auxiliary device for precisely cutting gels of the present utility model is mainly for directly and precisely cutting gels without removing the gels from the gel-making glass plates during the process of cutting electrophoresis gels in protein immunoblotting experiments, thereby reducing experimental errors.
[0082] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be construed as a limitation on the protected content of the present utility model.
[0083] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present utility model, rather than to limit them; although the present utility model has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present utility model.
Claims
1. An auxiliary device for precise gel cutting, characterized in that: include: cassette; A left rotating shaft and a right rotating shaft installed on the front and rear box walls of the dark box; A left blackout cloth reel mounted on a left rotating shaft; A left crossbar installed on the edge of the blackout cloth at the front end of the left blackout cloth reel; A right shade cloth reel mounted on a right rotating shaft; A right crossbar installed on the edge of the shade cloth at the front end of the right shade cloth reel; A rear shaft and a front shaft installed on the left and right walls of the dark box; A rear blackout cloth reel mounted on a rear rotating shaft; A rear crossbar installed on the edge of the blackout cloth at the front end of the rear blackout cloth reel; A front blackout cloth reel mounted on a front rotating shaft; A front crossbar installed on the edge of the blackout cloth at the front end of the front blackout cloth reel; A light source installed on the inner box wall behind the dark box; A glass plate mounted on the upper end of the cassette; A pressure plate mounted above the glass panel; A fixing groove disposed on the pressing plate; A transverse moving groove is arranged on the pressing plate, the transverse moving groove is located in front of the fixed groove and passes through the pressing plate and the glass plate; A vertical moving groove is arranged on the pressing plate, and the horizontal moving groove is located on the left side of the fixed groove and passes through the pressing plate and the glass plate; A first sliding assembly and a second sliding assembly installed in the transverse moving groove, wherein the first sliding assembly is connected to the left cross bar, and the second sliding assembly is connected to the right cross bar; A third sliding assembly and a fourth sliding assembly are installed in the vertical moving groove, wherein the third sliding assembly is connected to the rear cross bar, and the fourth sliding assembly is connected to the front cross bar.
2. The auxiliary device for precise gel cutting according to claim 1, characterized in that: The first sliding assembly, the second sliding assembly, the third sliding assembly and the fourth sliding assembly have the same structural dimensions.
3. The auxiliary device for precise gel cutting according to claim 1, characterized in that: The first sliding component is composed of a connecting plate, a connecting rod, a slider, a slider bottom plate, a slider top plate, a slider handle, and a pointer. The slider is arranged in a transverse moving groove, a slider top plate is installed above the slider, a pointer is arranged on the slider top plate, an edge of the slider top plate is located above the pressure plate surface, a slider bottom plate is installed below the slider, an edge of the slider bottom plate is located below the glass plate, and the slider, the slider bottom plate and the slider top plate are in an I-shaped structure and slide in the transverse moving groove.
4. An auxiliary device for precise gel cutting according to claim 1 or 2, characterized in that: The connecting plate on the first sliding assembly is connected to the left cross bar, the connecting plate on the second sliding assembly is connected to the right cross bar, the connecting plate on the third sliding assembly is connected to the rear cross bar, and the connecting plate on the fourth sliding assembly is connected to the front cross bar.
5. The auxiliary device for precise gel cutting according to claim 1, characterized in that: The light source is a long straight strip luminous body with uniform brightness, and the light source is horizontally installed on the rear inner box wall of the dark box.
6. The auxiliary device for precise gel cutting according to claim 1, characterized in that: The glass plate is made of a rectangular transparent material.
7. The auxiliary device for precise gel cutting according to claim 1, characterized in that: The pressing plate is made of non-transparent material, and the length and width of the pressing plate are the same as those of the glass plate and are in close contact with the glass plate.
8. The auxiliary device for precise gel cutting according to claim 1, characterized in that: The edge of the horizontal moving slot close to one side of the fixed slot is provided with a horizontal scale line, and the edge of the vertical moving slot close to one side of the fixed slot is provided with a vertical scale line.
9. The auxiliary device for precise gel cutting according to claim 3, characterized in that: The pointer of the first sliding assembly and the pointer of the second sliding assembly point to the horizontal scale line, and the pointer of the third sliding assembly and the pointer of the fourth sliding assembly point to the vertical scale line.