Elisa plate and enzyme immunoassay detector

Abstract

The utility model is suitable for the field of medical experiment equipment, and particularly relates to an elisa plate and an enzyme immunoassay detector. The base is provided with a cup position for placing the independent reaction cup, and the bottom of the cup position is provided with a shielding piece for preventing the independent reaction cup from falling off. According to the ELISA plate disclosed by the utility model, the independent reaction cups are adopted, and the shielding piece is arranged at the bottom of the base, so that the independent reaction cups can be taken under the condition that the anti-falling effect of the ELISA plate is ensured.

Description

Technical Field

[0001] The utility model belongs to the field of medical experimental equipment, and particularly relates to an ELISA plate and an enzyme immunoassay detector. Background Art

[0002] At present, as an important carrier for enzyme-linked immunosorbent assay, the ELISA plate not only serves as a reaction vessel, but also coats antigens / antibodies in its reaction micro-wells and participates in the test as part of the reagent.

[0003] ELISA plates are usually divided into detachable and non-detachable types. Among them, in the non-detachable ELISA plate, the reaction cups of the whole plate are connected as a whole, while in the detachable ELISA plate, the reaction cups are arranged in strips, and multiple strips are connected respectively to form a plate strip. Whether it is the reaction cups of the non-detachable ELISA plate or the detachable ELISA plate, generally multiple reaction cups need to be taken at one time during use. When the sample volume for detection is small, it is very easy to cause waste of reaction cups. Summary of the Utility Model

[0004] An embodiment of the utility model provides an ELISA plate, aiming to solve the problem of waste of reaction cups in the existing ELISA plates.

[0005] In a first aspect, the ELISA plate provided by the embodiment of the utility model includes:

[0006] At least one independent reaction cup, with reagents coated on the bottom of the independent reaction cup, and different types of reagents are coated for different detection items; and

[0007] A base, on which cup positions for placing the independent reaction cups are provided, and a shielding member for preventing the independent reaction cups from falling out is provided at the bottom of the cup positions. The distance between the shielding member and the entrance of the cup position is a fixed value, and the fixed value is less than the height of the independent reaction cup.

[0008] Optionally, the inner diameter of the inscribed circle defined by the inner wall of the cup position is greater than the outer diameter of the independent reaction cup;

[0009] A limiting structure is provided on the inner wall of the cup position, and the inscribed circle defined by the limiting structure is in interference fit with the independent reaction cup.

[0010] Optionally, the inner diameter of the inscribed circle defined by the inner wall of the cup position is greater than the outer diameter of the independent reaction cup;

[0011] A limiting structure is provided on the outer wall of the independent reaction cup, and the inscribed circle defined by the limiting structure and the inner wall of the cup position is in interference fit.

[0012] Optionally, the limiting structure is arranged close to the shielding member.

[0013] Optionally, the shielding member is a circular boss provided along the bottom of the cup position.

[0014] Optionally, the shielding member is a boss extending from one side wall of the cup position to the opposite side wall.

[0015] Optionally, the shielding member is a crossbar connecting one side wall of the cup position and the opposite side wall.

[0016] Optionally, the cup positions are arranged in a rectangular array.

[0017] Optionally, the outer wall of the reaction cup is frustum-shaped, and the angle between the generatrix of the frustum and the vertical direction is less than or equal to 2°.

[0018] In a second aspect, an enzyme immunoassay detector provided by an embodiment of the present invention includes the above-mentioned enzyme label plate used for the detection of the enzyme immunoassay detector.

[0019] The beneficial effects achieved by the present invention:

[0020] By adopting independent reaction cups and providing a shielding member at the bottom of the cup position, the reaction cups in the enzyme label plate can be independently taken out, and the independent reaction cups will not fall out from the bottom of the cup position when located in the base cup position, facilitating the access to individual reaction cups. Moreover, the distance between the shielding member and the entrance of the cup position is a fixed value, restricting the depth of the reaction tube placed in the tube rack to be consistent, thereby ensuring the accuracy of reagent coating during the production process and the reaction cup scheduling process during use. It solves the problem of waste of reaction cups or reagents caused during the detection of small sample volumes. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is an overall and corresponding cross-sectional structure schematic diagram of an enzyme label plate provided by an embodiment of the present invention.

[0022] Figure 2 It is an overall and corresponding cross-sectional structure schematic diagram of another enzyme label plate provided by an embodiment of the present invention.

[0023] Figure 3 It is an overall and corresponding cross-sectional structure schematic diagram of yet another enzyme label plate provided by an embodiment of the present invention.

[0024] Reference numerals in the drawings: 100, enzyme label plate; 10, base; 11, cup position; 111, shielding member; 112, limiting structure; 20, independent reaction cup. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0025] In order to make the objectives, technical solutions and advantages of the present utility model more clear and understandable, the present utility model will be further described in detail below in conjunction with the accompanying drawings and embodiments. The examples of the embodiments are shown in the accompanying drawings, where the same or similar reference numerals denote the same or similar elements or elements with the same or similar functions from beginning to end. The embodiments described below by referring to the accompanying drawings are exemplary and are only used to explain the present utility model and should not be construed as a limitation to the present utility model. In addition, it should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0026] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "left", "right", "horizontal", "top", "bottom", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present utility model.

[0027] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of the said features. In the description of the present utility model, "a plurality" means two or more, unless otherwise specifically defined.

[0028] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection or an integral connection; it may be a mechanical connection, an electrical connection or a connection capable of mutual communication; it may be directly connected or indirectly connected through an intermediate medium, and may be the internal communication of two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0029] In the present utility model, unless otherwise clearly defined and limited, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through other features therebetween. Moreover, the first feature being "above", "over" and "on the top of" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "below", "under" and "beneath" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely indicates that the horizontal height of the first feature is lower than that of the second feature.

[0030] The following disclosure provides many different embodiments or examples for implementing different structures of the present utility model. To simplify the disclosure of the present utility model, the components and settings of specific examples are described hereinafter. Of course, they are merely examples and are not intended to limit the present utility model. In addition, the present utility model may repeat reference numerals and / or reference letters in different examples. Such repetition is for the purpose of simplification and clarity, and does not itself indicate the relationship between various embodiments and / or settings discussed. In addition, the present utility model provides examples of various specific processes and materials, but those of ordinary skill in the art can be aware of the application of other processes and / or the use of other materials.

[0031] The enzyme-labeled plate provided by the present utility model, by adopting independent reaction cups and arranging a shielding member at the bottom of the cup position, enables the reaction cups in the enzyme-labeled plate to be independently taken out, and the independent reaction cups will not fall out from the bottom of the cup position when located in the cup positions of the base, facilitating the access to individual reaction cups. Moreover, the distance between the shielding member and the entrance of the cup position is a fixed value, restricting the depth of the reaction tubes inserted into the tube rack to be consistent, thereby ensuring the accuracy of reagent coating during the production process and the reaction cup scheduling process during use. It solves the problem of waste of reaction cups or reagents during small sample volume detection.

[0032] Embodiment

[0033] The present utility model provides an enzyme-labeled plate 100, comprising:

[0034] At least one independent reaction cup 20, with reagents coated on the bottom of the independent reaction cup 20, and different reagent types are coated for different detection items; and

[0035] A base 10, on which cup positions 11 for placing the independent reaction cups 20 are arranged, and a shielding member 111 for preventing the independent reaction cups 20 from falling out is arranged at the bottom of the cup positions 11. The distance between the shielding member 111 and the entrance of the cup position 11 is a fixed value, and this fixed value is less than the height of the independent reaction cup 20.

[0036] In the prior art, the reaction cups in the microtiter plate 100 are usually in a structure where multiple cups are connected. That is, at the position near the cup mouth of each reaction cup, there is an extension part connected to the adjacent reaction cup. The shape of the extension part can be a flat and long strip or the like. The adjacent reaction cups are connected to each other through the extension part, so that multiple reaction cups are arranged in a strip shape or in a rectangular array. Therefore, when in use, taking out one reaction cup will bring out the whole strip or the whole plate of reaction cups connected to it.

[0037] In contrast, at the cup mouth of the independent reaction cup 20, there is no extension part for connecting adjacent reaction cups. Therefore, it can be taken out separately from the microtiter plate 100.

[0038] The microtiter plate 100 provided in this embodiment has a base 10 for placing the independent reaction cup 20. In an experiment where the number of reaction cups required is small, the independent reaction cup 20 can be taken out separately from the microtiter plate 100 for use.

[0039] Specifically, please refer to Figure 1 , the base 10 provides limit and support for the reaction cup 20. The base 10 can be a flat plate with a certain thickness, and there are vertical through holes penetrating the upper and lower surfaces of the flat plate. A number of cup positions 11 can be set and are evenly arranged on the base 10. Since in actual use, the number of reaction cups required for different experiments is different, according to the number of reaction cups specifically needed in the experiment, an appropriate number of reaction cups are placed in the positions, and the cup positions without reaction cups placed are left vacant. It can be understood that the inner diameter of the cup position 11 is larger than the outer diameter of the independent reaction cup 20 to ensure that the independent reaction cup 20 can be smoothly placed in the cup position 11.

[0040] In some embodiments, the cup position 11 can be a cylindrical through hole. In other embodiments, the cup position 11 can also be a rectangular through hole or a triangular through hole or other polygonal through holes, which are not specifically limited here.

[0041] In this embodiment, a blocking member 111 for preventing the reaction cup from coming out is further provided at the bottom of the cup position 11. When the cup position 11 is a blind hole, the blocking member 111 is the bottom of the blind hole. When the cup position 11 is a through hole, the blocking member 111 is arranged at the bottom of the through hole to form a block for the bottom of the through hole.

[0042] The outer diameter of the independent reaction cup 20 is smaller than the inner diameter of the cup position 11. When the independent reaction cup 20 is placed in the cup position 11, there is a risk of directly coming out of the cup position 11. The blocking member 111 is arranged at the bottom of the cup position 11 to form an obstacle on the possible sliding path of the independent reaction cup 20, so as to limit the independent reaction cup 20 and prevent the independent reaction cup 20 from coming out from the bottom of the cup position 11.

[0043] The distance between the shielding member 111 and the entrance of the cup position 11 is a fixed value, and the fixed value is less than the height of the independent reaction cup 20, that is, the shielding member 111 limits the depth of the reaction tube placed in the tube rack to remain consistent, thereby ensuring the accuracy of the reagent coating process during the production process and the reaction cup scheduling process during use.

[0044] Based on the above structure, different reaction cups in the ELISA plate 100 are independent of each other, that is, each reaction cup is not provided with a connecting structure, so that the reaction cup can be taken individually and the waste caused by the reaction cup not being able to be taken as needed is avoided; at the same time, a shielding member 111 is also provided at the bottom of the cup position 11 where the independent reaction cup 20 is placed. When the independent reaction cup 20 enters the cup position 11, the bottom of the independent reaction cup 20 is blocked by the shielding member 111 when it reaches the bottom of the cup position 11, and stops sliding down, thereby avoiding the independent reaction cup 20 directly falling out from the bottom of the cup position 11.

[0045] In some embodiments, the diameter of the inscribed circle defined by the inner wall of the cup position 11 is larger than the outer diameter of the independent reaction cup 20;

[0046] A limiting structure 112 is disposed on the inner wall of the cup position 11 , and the inscribed circle defined by the limiting structure 112 is interference-fitted with the independent reaction cup 20 .

[0047] In this embodiment, the area enclosed by the inner wall of the cup position 11 should be larger than the area enclosed by the outer wall of the independent reaction cup 20. When the cup position 11 is a circular hole, the diameter of the circular hole is larger than the outer diameter of the independent reaction cup 20. When the cup position is a square hole, a triangular hole or other hole with straight edges, the diameter of the circle tangent to the inner wall of the cup position 11 is larger than the outer diameter of the independent reaction cup 20.

[0048] The limiting structure 112 can be set on the inner wall of the cup position 11, the limiting structure 112 protrudes from the inner wall of the cup position 11, and the inscribed circle defined by the limiting structure 112 is interference-fitted with the independent reaction cup 20, so that the reaction cup 20 is clamped between the limiting structures. The limiting structure 112 can be a convex point, a convex ring, or other structures protruding from the inner wall of the cup position 11.

[0049] Specifically, when the cup seat 11 is a circular hole, the limiting structure 112 may be at least two protrusions arranged along the inner wall of the circular hole. When the cup seat 11 is a square hole or a triangular hole, the limiting structure 112 may be one protrusion arranged on each side wall.

[0050] The limiting structure 112 can be integrally formed with the cup seat 11 .

[0051] Based on the above structure, when the independent reaction cup 20 is placed in the cup position 11, the independent reaction cup 20 is in interference fit with the limiting structure 112, and the independent reaction cup 20 is limited, so as to prevent the independent reaction cup 20 from shaking when the microplate is moved. Moreover, the outer wall of the independent reaction cup 20 forms a point contact with the limiting structure 112, with a small contact area and small friction, making it easier and more labor-saving to access the independent reaction cup 20.

[0052] In some embodiments, the diameter of the inscribed circle defined by the inner wall of the cup position 11 is greater than the outer diameter of the independent reaction cup 20;

[0053] A limiting structure 112 is provided on the outer wall of the independent reaction cup 20, and the limiting structure 112 is in interference fit with the inscribed circle defined by the inner wall of the cup position 11.

[0054] The limiting structure 112 can be provided on the outer wall of the independent reaction cup 20. The limiting structure 112 protrudes from the outer wall of the reaction cup 20, and the limiting structure 112 is in interference fit with the inscribed circle defined by the inner wall of the cup position 11, clamping the reaction cup 20 between the inner walls of the cup position 11. The limiting structure 112 can be a convex point, a convex ring or other structures protruding from the inner wall of the cup position 11.

[0055] Specifically, the limiting structure 112 can be provided with at least two convex points along the outer wall of the reaction cup 20, or can be a convex ring provided in a circle along the outer wall of the reaction cup.

[0056] Among them, the limiting structure 112 can be integrally formed with the reaction cup 20.

[0057] In some embodiments, the limiting structure 112 is provided near the bottom of the cup position 11, and the height of the protrusion of the limiting structure 112 is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0058] In this embodiment, the limiting structure 112 is provided on the inner wall of the cup position 11, and the limiting structure 112 is located at one end of the inner wall of the cup position 11 close to the cup position 11. The limiting structure 112 can be four convex points evenly distributed around the inner wall. In other embodiments, the limiting structure can be other structures.

[0059] The shielding member 111 blocks the independent reaction cup 20 to prevent the independent reaction cup 20 from moving downward continuously. If the limiting structure 112 is provided in the upper part of the cup position 11, when the independent reaction cup 20 is placed, the contact path between the independent reaction cup 20 and the limiting structure 112 is long, the friction is large, and it is easy to scratch the outer wall of the independent reaction cup 20. The limiting structure 112 is provided at a position close to the shielding member 111, the contact path between the independent reaction cup 20 and the limiting structure 112 is short, which is convenient for the independent reaction cup 20 to be pulled out, and also avoids the limiting structure 112 from scratching the outer wall of the reaction cup 20.

[0060] The height of the protrusion of the limiting structure 112 is greater than or equal to 0.1 mm and less than or equal to 0.5 mm. If the height of the protrusion of the limiting structure 112 is less than 0.1 mm, it cannot effectively have an interference fit with the independent reaction cup 20. If the height of the protrusion of the limiting structure 112 is greater than 0.5 mm, it may block the placement of the independent reaction cup 20 into the cup position 11.

[0061] Based on the above structure, when the reaction cup enters the cup position 11, it does not come into contact with the limiting structure 112 first. It is not until the reaction cup reaches the bottom of the cup position 11 that the reaction cup comes into contact with the limiting structure 112. The cup position 11 fixes the reaction cup through the limiting structure 112, avoiding the premature contact between the reaction cup and the limiting structure 112, reducing unnecessary friction, and making the access of the reaction cup more convenient and labor-saving.

[0062] In some embodiments, the limiting structure 112 is arranged near the mouth of the independent reaction cup 20, and the height of the protrusion of the limiting structure 112 is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

[0063] In this embodiment, the limiting structure 112 is arranged on the outer wall of the independent reaction cup 20, and the limiting structure 112 is located at one end of the inner wall of the cup position 11 close to the shielding member 111. The limiting structure 112 can be four bump points evenly distributed around the inner wall. In other embodiments, the limiting structure can be other structures.

[0064] The shielding member 111 blocks the independent reaction cup 20 to prevent the independent reaction cup 20 from moving downward continuously. If the limiting structure 112 is arranged at the upper part of the cup position 11, the contact path between the inner wall of the cup position 11 and the limiting structure 112 is short, which is convenient for the independent reaction cup 20 to be pulled out and also avoids the inner wall of the cup position 11 being scratched by the limiting structure 112.

[0065] The height of the protrusion of the limiting structure 112 is greater than or equal to 0.1 mm and less than or equal to 0.5 mm. If the height of the protrusion of the limiting structure 112 is less than 0.1 mm, it cannot effectively have an interference fit with the independent reaction cup 20. If the height of the protrusion of the limiting structure 112 is greater than 0.5 mm, it may block the placement of the independent reaction cup 20 into the cup position 11.

[0066] Based on the above structure, when the reaction cup enters the cup position 11, the inner wall of the cup position 11 does not come into contact with the limiting structure 112 first. It is not until the reaction cup reaches the bottom of the cup position 11 that the inner wall of the cup position 11 comes into contact with the limiting structure 112. The independent reaction cup 20 is fixed to the cup position 11 through the limiting structure 112, avoiding the premature contact between the inner wall of the cup position 11 and the limiting structure 112, reducing unnecessary friction, and making the access of the reaction cup more convenient and labor-saving.

[0067] In some embodiments, the shielding member 111 is a circular protrusion arranged along the bottom of the cup position 11.

[0068] Please refer to Figure 1 , in one embodiment, the blocking member 111 may be a boss that protrudes inwardly in a circle on the inner wall of the cup position 11 and is located at one end near the bottom in the cup position 11.

[0069] Specifically, the area enclosed by the top of the boss should be smaller than the area enclosed by the outer diameter of the independent reaction cup 20. If the area enclosed by the top of the boss is greater than or equal to the area enclosed by the outer diameter of the independent reaction cup 20, the reaction cup may slip out of the gap of the blocking member 111, causing the blocking member 111 to lose its anti-slip function.

[0070] Based on the above structure, when the reaction cup enters the cup position 11 and reaches the bottom, since the space enclosed by the boss is smaller than the space enclosed by the outer wall of the reaction cup, the anti-slip function of preventing the reaction cup from slipping out is achieved only by the circle of bosses at the bottom of the cup position 11, reducing the material loss of producing the blocking member 111 and saving the processing cost of the ELISA plate 100.

[0071] In some embodiments, the blocking member 111 is a boss that extends from one side wall of the cup position 11 to the opposite side wall.

[0072] Please refer to Figure 2 , in one embodiment, the blocking member 111 may be a boss that extends inwardly in a section on the inner wall of the cup position 11, and the boss may be located on one side at one end near the bottom in the cup position 11.

[0073] Specifically, in this embodiment, the length of the protruding part of the blocking member 111 may extend from one side to the center position of the hole, covering half of the space at the bottom of the cup position 11. The blocking member 111 may also extend from one side to the other side to completely enclose the bottom of the cup position 11. In other embodiments, the blocking member 111 may also protrude different lengths to achieve different blocking effects on the bottom of the cup position 11, but it is necessary to ensure that the reaction cup will not slip out along the remaining space at the bottom of the cup position 11, which is not specifically limited herein.

[0074] Based on the above structure, when the reaction cup reaches the bottom of the cup position 11, since there is a boss extending inwardly at the bottom of the cup position 11, the remaining space at the bottom of the cup position 11 cannot allow the reaction cup to pass through. Therefore, the effect of preventing the reaction cup from slipping out is achieved by setting the blocking member 111 as a boss extending from the bottom of the cup position 11.

[0075] In some embodiments, the blocking member 111 is a crossbar connecting one side wall of the cup position 11 and the opposite side wall.

[0076] Please refer to Figure 3In one embodiment, the blocking member 111 may be a crossbar connecting two opposite sidewalls. In this way, the bottom of the cup position 11 can be divided into two blocks, and the area of any block is smaller than the area of the bottom of the outer diameter of the independent reaction cup 20, so that the outer diameter of the independent reaction cup 20 cannot escape therefrom. The crossbar may also have a certain width, so that a plane can be formed at the top to provide support when the independent reaction cup 20 is placed. When the cup position 11 is square, the blocking member may connect the two diagonals of the square to form a blockage.

[0077] In another embodiment, the blocking member 111 may be connected to the bottom of the cup position 11 by a snap connection. The two ends of the blocking member 111 are respectively connected to both sides of the inner wall of the cup position 11. In other embodiments, the blocking member 111 may also be of other shapes, such as a flat and long plate shape, etc., and the connection method may also be other methods, such as an integrated design, etc.

[0078] Based on the above structure, when the bottom of the reaction cup reaches the bottom of the cup position 11, the bottom of the reaction cup contacts the blocking member 111, and the reaction cup stops sliding down to prevent the reaction cup from escaping. Using a crossbar as the blocking member 111 facilitates connecting the blocking member 111 and the cup position 11 by a snap connection to achieve a detachable connection, so that the blocking member 111 can be replaced when it is damaged.

[0079] In some embodiments, the cup positions 11 are arranged in a rectangular array.

[0080] In this embodiment, a number of longitudinal auxiliary lines parallel to the width direction may be provided in the base 10, and the distances between the number of longitudinal auxiliary lines are equal, and this distance should be greater than the length occupied by one cup position 11; similarly, a number of transverse auxiliary lines parallel to the length direction are provided in the base 10, and the distances between the number of transverse auxiliary lines are equal, and this distance should also be greater than the width occupied by one cup position 11. The cup positions 11 can be approximately arranged at the intersections of the above lines. In other embodiments, other methods may also be used to position the cup positions 11 to achieve a rectangular array arrangement.

[0081] Based on the above structure, reaction cups in different situations can be classified according to rows or columns, providing clear guidance for the placement and retrieval positions of the independent reaction cups 20, and improving the efficiency of retrieving and labeling the independent reaction cups 20.

[0082] In one embodiment, 8 rows can be arranged in the width direction at the bottom, and 6 cup positions 11 are provided in each row and are equal. The distances between the respective cup positions 11 can be approximately equal, or the row spacing and column spacing can be arranged in an equal manner respectively, and no specific limitation is made here.

[0083] In one embodiment, 8 rows can be arranged in the width direction at the bottom, and 12 cup positions 11 are provided in each row. The cup positions 11 are equal, and the distances between the cup positions 11 can be approximately equal, or they can be arranged in a manner where the row spacing and column spacing are respectively equal. No specific limitation is made here.

[0084] Based on the above structure, it can be applied to most enzyme immunoassay detectors of disease control and scientific research institutes, etc. Using the enzyme label plate 100 of this embodiment does not require the configuration of a special model of enzyme immunoassay detector, reducing the usage cost.

[0085] In some embodiments, the outer wall of the independent reaction cup 20 is frustum-shaped, and the angle between the generatrix of the frustum and the vertical direction is less than or equal to 2°.

[0086] In this embodiment, the large end of the frustum formed by the outer wall of the independent reaction cup 20 is close to the cup mouth of the reaction cup, and the small end is close to the cup bottom of the reaction cup. The angle between the generatrix of the frustum and the vertical direction does not exceed 2°. A certain slope is set on the outer wall of the independent reaction cup 20 to facilitate the insertion or removal of the independent reaction cup 20. And because the diameter of the independent reaction cup 20 is small, if the angle between the generatrix of the frustum and the vertical direction is too large, the bottom area of the independent reaction cup 20 will be too small, which is not conducive to coating the reaction reagent. The angle between the generatrix of the frustum and the vertical direction does not exceed 2° to ensure that the bottom area of the independent reaction cup 20 is sufficient to coat the reaction reagent.

[0087] In some embodiments, the outer diameter of the bottom of the independent reaction cup 20 is greater than or equal to 7.5 mm and less than or equal to 8.5 mm; the inner diameter of the bottom of the independent reaction cup 20 is greater than or equal to 5.7 mm and less than or equal to 6.7 mm; the diameter of the inscribed circle defined by the inner wall of the cup position 11 is greater than or equal to 7.5 mm and less than or equal to 8.5 mm.

[0088] The frictional force between the independent reaction cup and the cup position is greater than or equal to 0.5 N and less than or equal to 2 N. If the frictional force is too large, the reaction cup cannot be taken out; if the frictional force is too small, the reaction cup cannot be clamped tightly and is likely to come out.

[0089] Preferably, the frictional force between the independent reaction cup and the cup position is greater than or equal to 0.5 N and less than or equal to 1 N.

[0090] The dimensions defined in this embodiment are adapted to the registration standards of existing enzyme label plates, without the need for re-registration, and the application cost is low.

[0091] In other embodiments, if re-registration is required, it is not limited to the above dimensions.

[0092] The present utility model provides an enzyme immunoassay detector, comprising:

[0093] A plate placement device for loading the above-mentioned enzyme label plate;

[0094] A cup-taking device is used to take out a corresponding number of independent reaction cups from an ELISA plate according to the number of samples to be detected, so as to pour the samples to be detected into the independent reaction cups for reaction and detection. The number of samples to be detected by the ELISA detector corresponds to the number of reaction cups taken out from the ELISA plate.

[0095] In this embodiment, the ELISA plate 100 is used for the detection of the ELISA detector. The ELISA plate is placed on the plate placement device. The cup-taking device takes out a corresponding number of reaction cups from the ELISA plate according to the number of samples detected by the ELISA detector, places them on the empty detection plate, and then uses the detection plate carrying the reaction cups for detection. The number of reaction cups used matches the number of samples, without causing waste of reaction cups. It can be understood that those skilled in the art can combine various implementation manners in the above embodiments under the guidance of the above embodiments to obtain technical solutions of various implementation manners.

[0096] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An ELISA plate, characterized in that: include: At least one independent reaction cup, the bottom of the independent reaction cup is coated with reagents, and different detection items are coated with different types of reagents; as well as A base is provided with a cup position for placing the independent reaction cup, a shielding member is provided at the bottom of the cup position to prevent the independent reaction cup from escaping, and the distance between the shielding member and the cup position entrance is a fixed value, and the fixed value is less than the height of the independent reaction cup.

2. The ELISA plate according to claim 1, characterized in that The diameter of the inscribed circle defined by the inner wall of the cup position is greater than or equal to the outer diameter of the independent reaction cup; A limiting structure is provided on the inner wall of the cup, and the inscribed circle defined by the limiting structure has an interference fit with the independent reaction cup, or a limiting structure is provided on the outer wall of the independent reaction cup, and the limiting structure has an interference fit with the inscribed circle defined by the inner wall of the cup.

3. The ELISA plate according to claim 2, characterized in that The limiting structure is arranged close to the bottom of the cup, and the height of the protrusion of the limiting structure is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

4. The ELISA plate according to claim 2, characterized in that The limiting structure is arranged close to the mouth of the independent reaction cup, and the height of the protrusion of the limiting structure is greater than or equal to 0.1 mm and less than or equal to 0.5 mm.

5. The ELISA plate according to claim 1, characterized in that The shielding member is a circle of bosses arranged along the bottom of the cup.

6. The ELISA plate according to claim 1, characterized in that The shielding member is a boss extending from one side wall of the cup position to the opposite side wall.

7. The ELISA plate according to claim 1, characterized in that The shielding member is a horizontal piece connecting a side wall and an opposite side wall of the cup position.

8. The ELISA plate according to claim 1, characterized in that The outer wall of the independent reaction cup is in the shape of a truncated cone, and the angle between the generatrix of the truncated cone and the vertical direction is less than or equal to 2°.

9. The ELISA plate according to claim 1, characterized in that The outer diameter of the bottom of the independent reaction cup is greater than or equal to 7.5 mm and less than or equal to 8.5 mm; The inner diameter of the bottom of the independent reaction cup is greater than or equal to 5.7 mm and less than or equal to 6.7 mm; The diameter of the inscribed circle defined by the inner wall of the cup is greater than or equal to 7.5 mm and less than or equal to 8.5 mm.

10. An enzyme immunoassay tester, characterized in that: The enzyme immunoassay detector comprises: A plate placement device, used for loading the ELISA plate as claimed in any one of claims 1 to 9; The cup taking device is used to take out a corresponding number of independent reaction cups from the ELISA plate according to the number of samples to be tested, so as to add the samples to be tested into the independent reaction cups for reaction and detection.

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