Fluorescence detection member and fluorescence detection device

Abstract

The utility model belongs to the technical field of fluorescence detection, and particularly relates to a fluorescence detection piece and a fluorescence detection device.The fluorescence detection piece comprises an excitation part internally provided with an excitation channel and an emission part internally provided with an emission channel, the emission channel and the excitation channel are arranged in a separated mode, and the central axis of the emission channel intersects with the central axis of the excitation channel. During detection, a to-be-detected sample needs to be placed at the intersection of the central axis of the excitation channel and the central axis of the emission channel, then excitation light is emitted to the to-be-detected sample through the fluorescence excitation assembly in the excitation channel, and the to-be-detected sample can emit emission light after being irradiated by the excitation light; and finally, the fluorescence emission assembly in the emission channel receives the emitted light and analyzes and detects the emitted light. In the application, the central axis of the emission channel and the central axis of the excitation channel intersect to avoid coaxial arrangement of the excitation light and the emission light, so that the influence of the excitation light on the emission light is reduced, and the sensitivity of detecting the to-be-detected sample is improved.

Description

Technical Field

[0001] This application belongs to the technical field of fluorescence detection, and particularly relates to a fluorescence detection component and a fluorescence detection device. Background Art

[0002] Real-time fluorescence quantitative PCR technology is a relatively mainstream gene detection technology at present. After the sample to be tested is irradiated by the excitation light, it will emit emission light with a longer wavelength than the excitation light. The fluorescence detection device uses a detector to detect the emission light, converts the optical signal into an electrical signal, and finally obtains the intensity of the fluorescence emitted by the sample to be tested by analyzing the electrical signal.

[0003] In the prior art, an excitation light source is used to emit excitation light to the sample to be tested. The sample to be tested emits emission light while being irradiated by the excitation light. The detector needs to detect the emission light to realize the detection of the sample to be tested. Among them, the excitation light source and the detector are located in the same cavity. For the above-mentioned related technologies, the excitation light will be detected by the detector at the same time, thus affecting the detection of the emission light by the detector, and further reducing the sensitivity of the detection of the sample to be tested. Summary of the Utility Model

[0004] The purpose of this application is to provide a fluorescence detection component and a fluorescence detection device, aiming to improve the detection sensitivity.

[0005] To achieve the above purpose, on the one hand, this application provides a fluorescence detection component, including:

[0006] An excitation part, which is provided with an excitation channel inside, and a first opening for installing a fluorescence excitation component is arranged on the peripheral wall of the excitation channel; and

[0007] An emission part, which is connected to the excitation part and is provided with an emission channel inside. The emission channel is arranged separately from the excitation channel. The central axis of the emission channel intersects the central axis of the excitation channel. A second opening for installing a fluorescence emission component is arranged on the peripheral wall of the emission channel.

[0008] In some embodiments, a plurality of first insertion slots that are axially spaced and communicate with the first opening are formed on the inner peripheral wall of the excitation channel, and a plurality of second insertion slots that are axially spaced and communicate with the second opening are formed on the inner peripheral wall of the emission channel.

[0009] In some embodiments, the notch of the first insertion slot faces perpendicular to the central axis of the excitation channel, and the notch of the second insertion slot faces perpendicular to the central axis of the emission channel.

[0010] In some embodiments, the fluorescence detection member includes a plurality of the excitation portions and a plurality of the emission portions arranged in one-to-one correspondence with the plurality of the excitation portions. The plurality of the excitation portions are arranged in sequence along the radial direction of the excitation channel, and the plurality of the emission portions are arranged in sequence along the radial direction of the emission channel.

[0011] In some embodiments, the first opening is provided on the peripheral wall of the excitation channel on the side away from the emission portion, and the second opening is provided on the peripheral wall of the emission channel on the side away from the excitation portion; and / or,

[0012] A first cover body is covered on the first opening, and a second cover body is covered on the second opening

[0013] In some embodiments, the excitation portion and the emission portion are integrally formed; and / or,

[0014] The excitation portion and the emission portion are connected to form a V-shaped structure.

[0015] In some embodiments, a detection space is formed at the intersection point of the central axis of the emission channel and the central axis of the excitation channel, and the detection space is used to accommodate a detection carrier.

[0016] The second aspect of the present application provides a fluorescence detection device, including:

[0017] A fluorescence detection member;

[0018] A fluorescence excitation assembly, installed in the excitation channel; and

[0019] A fluorescence emission assembly, installed in the emission channel.

[0020] In some embodiments, the fluorescence detection device further includes a detection carrier for loading a sample to be detected. In the use state of the fluorescence detection device, the detection carrier is located in the detection space between the excitation portion and the emission portion and is arranged at an interval from the fluorescence detection member.

[0021] In some embodiments, the fluorescence excitation assembly includes an excitation light source, an excitation collimating lens, an excitation filter, and an excitation focusing lens arranged in sequence and in optical communication. The centers of the excitation light source, the excitation collimating lens, the excitation filter, and the excitation focusing lens are all located on the central axis of the excitation channel; and / or,

[0022] The fluorescence emission assembly includes an emission collimating lens, an emission filter, an emission focusing lens, and a detector arranged in sequence and in optical communication. The centers of the emission collimating lens, the emission filter, the emission focusing lens, and the detector are all located on the central axis of the emission channel.

[0023] With the above technical solutions, the fluorescence detection element and the fluorescence detection device provided by the present application have the following

[0024] Advantageous effects:

[0025] During detection, it is necessary to place the detection carrier containing the sample to be detected at the intersection of the central axis of the excitation channel and the central axis of the emission channel. Then, the fluorescence excitation component in the excitation channel emits excitation light to the sample to be detected. After being irradiated by the excitation light, the sample to be detected emits emission light with a wavelength longer than that of the excitation light. Then, the emission light enters the emission channel, and finally, the fluorescence emission component in the emission channel receives the emission light and analyzes it. In the present application, by arranging the central axis of the emission channel to intersect with the central axis of the excitation channel, the coaxial arrangement of the excitation light and the emission light is avoided, thereby reducing the influence of the excitation light on the emission light, and further improving the sensitivity of the detection of the sample to be detected.

[0026] Other features and advantages of the embodiments of the present application will be described in detail in the subsequent specific embodiment part. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The drawings are used to provide a further understanding of the embodiments of the present application, and constitute a part of the specification. They are used together with the following specific embodiments to explain the embodiments of the present application, but do not constitute a limitation to the embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on the structures shown in these drawings without creative efforts. In the drawings:

[0028] Figure 1 is a schematic structural diagram of a fluorescence detection element in a specific embodiment according to the present application;

[0029] Figure 2 is a schematic structural diagram of a fluorescence detection element including a plurality of excitation parts and a plurality of emission parts in a specific embodiment according to the present application;

[0030] Figure 3 is a schematic structural diagram of a fluorescence detection element excluding the cover in a specific embodiment according to the present application;

[0031] Figure 4 is a schematic structural diagram of another perspective of a fluorescence detection element excluding the cover in a specific embodiment according to the present application;

[0032] Figure 5 is a schematic structural diagram of a fluorescence excitation component in a specific embodiment according to the present application;

[0033] Figure 6 is a schematic structural diagram of a fluorescence emission component in a specific embodiment according to the present application;

[0034] Figure 7Schematic structural diagram of a fluorescence detection device with an integrated cartridge according to a specific embodiment of the present application;

[0035] Figure 8 Schematic structural diagram of a fluorescence detection device with a separable cartridge according to a specific embodiment of the present application;

[0036] Figure 9 Schematic optical path diagram of a fluorescence detection device according to a specific embodiment of the present application.

[0037] Description of reference numerals

[0038] 100 Fluorescence detection device 110 Fluorescence detection member

[0039] 120 Detection carrier 130 Fluorescence excitation assembly

[0040] 131 Excitation light source 132 Excitation collimating lens

[0041] 133 Excitation filter 134 Excitation focusing lens

[0042] 140 Fluorescence emission assembly 141 Emission collimating lens

[0043] 142 Emission filter 143 Emission focusing lens

[0044] 144 Detector 1 Excitation part

[0045] 2 Emission part 3 Excitation channel

[0046] 4 Emission channel 5 First opening

[0047] 6 Second opening 7 First cover

[0048] 8 Second cover 9 First insertion slot

[0049] 10 Second insertion slot 11 First mounting plate

[0050] 12 Second mounting plate 13 Detection space Detailed description of the specific implementation

[0051] The following is a detailed description of the specific implementation of the present application in conjunction with the accompanying drawings. It should be understood that the specific implementation described herein is only for the purpose of illustrating and explaining the present application and is not intended to limit the present application.

[0052] The following describes the noun parts of the fluorescence detection member 110 and the fluorescence detection device 100 according to the present application with reference to the accompanying drawings.

[0053] As Figures 1 to 6As shown in the figure, a specific embodiment of the present application provides a fluorescence detection component 110, including an excitation part 1 and an emission part 2. Among them, an excitation channel 3 is provided in the excitation part 1, and a first opening 5 for installing a fluorescence excitation component 130 is provided on the peripheral wall of the excitation channel 3; the emission part 2 is connected to the excitation part 1 and is internally provided with an emission channel 4. The emission channel 4 is arranged separately from the excitation channel 3. The central axis of the emission channel 4 intersects the central axis of the excitation channel 3. A second opening 6 for installing a fluorescence emission component 140 is provided on the peripheral wall of the emission channel 4.

[0054] When performing fluorescence detection on a sample to be tested, it is necessary to use the fluorescence excitation component 130 to emit excitation light to the sample to be tested. After the sample to be tested is irradiated by the excitation light, it will emit fluorescence. Then, use the fluorescence emission component 140 to receive the fluorescence emitted by the sample to be tested for detection. In the present application, the fluorescence excitation component 130 is located in the excitation channel 3 and the optical path of the fluorescence excitation component 130 is kept consistent with the central axis of the excitation channel 3. The fluorescence emission component 140 is located in the emission channel 4 and the optical path of the fluorescence emission component 140 is kept consistent with the central axis of the emission channel 4. By intersecting the central axis of the emission channel 4 with the central axis of the excitation channel 3, the excitation light and the emission light intersect and the sample to be tested is placed at the intersection of the excitation light and the emission light, so as to avoid coaxial setting of the excitation light and the emission light to reduce the influence of the excitation light on the emission light, thereby improving the sensitivity of detecting the sample to be tested.

[0055] Moreover, by providing the first opening 5 and the second opening 6 to install the fluorescence excitation component 130 and the fluorescence emission component 140, the disassembly and assembly of the fluorescence excitation component 130 and the fluorescence emission component 140 are facilitated, thereby improving the detection efficiency.

[0056] Unless otherwise stated, the orientation words such as "up, down, left, right, front, back" are usually in reference to the directions shown in the drawings. The position description word "inside" means located inside the component, and the position description word "outside" means located outside the component.

[0057] In some embodiments, the central axis of the emission part 2 extends in the up-down direction, and the central axis of the excitation part 1 intersects the central axis of the emission part 2 to form an intersection point and an included angle greater than 0° and less than 180°.

[0058] Specifically, the emission channel 4 penetrates through the emission part 2, and the axial direction of the emission channel 4 is consistent with the extension direction of the central axis of the emission part 2. The fluorescence emission component 140 is installed in the emission channel 4, and the optical path of the fluorescence emission component 140 coincides with the central axis of the emission part 2; the excitation channel 3 penetrates through the excitation part 1, and the axial direction of the excitation channel 3 is consistent with the extension direction of the central axis of the excitation part 1. The fluorescence excitation component 130 is installed in the excitation channel 3, and the optical path of the fluorescence excitation component 130 coincides with the central axis of the excitation part 1. By forming the fluorescence excitation channel 3 and the fluorescence emission channel 4 in the fluorescence detection component 110 to respectively install the fluorescence excitation component 130 and the fluorescence emission component 140, it is convenient for the movement and carrying of the fluorescence excitation component 130 and the fluorescence emission component 140.

[0059] Further, one end of the emission channel 4 close to the intersection point is the incident end, and one end of the emission channel 4 far from the intersection point is the detection end. The fluorescence emission component 140 receives the emission light entering from the incident end; one end of the excitation channel 3 close to the intersection point is the exit end, and one end of the excitation channel 3 far from the intersection point is the excitation end. The fluorescence excitation component 130 emits the excitation light from the exit end. A detection space 13 is formed at the intersection point of the central axis of the emission channel 4 and the central axis of the excitation channel 3. The detection space 13 is used to accommodate the detection carrier 120 containing the sample to be measured. By placing the sample to be measured at the intersection point and on the central axis of the emission channel 4, the reflected light emitted by the sample to be measured can enter the emission channel 4 as much as possible to be detected by the fluorescence emission component 140, thereby improving the detection sensitivity.

[0060] Preferably, the excitation part 1 is located on the left side of the emission part 2, and the included angle is an acute angle, so as to reduce the volume of the fluorescence detection component 110, thereby reducing the cost and facilitating carrying.

[0061] Specifically, the side walls of the excitation part 1 and the emission part 2 close to each other are connected, and the excitation part 1 and the emission part 2 are integrally formed to form a V-shaped structure. The excitation channel 3 and the emission channel 4 are independent of each other and do not communicate with each other, so as to avoid interference between the excitation light and the emission light.

[0062] Those skilled in the art can understand that the present application is not limited to the above structural form in which the excitation part 1 is arranged on the left side of the emission part 2 and extends from the lower left to the upper right. It can also be a structural form in which the excitation part 1 is arranged on any side of the emission part 2 and the extension direction of the excitation part 1 is not parallel to the extension direction of the emission part 2. Even it can be a structural form in which the excitation part 1 extends in the up and down direction and the emission part 2 is arranged on any side of the excitation part 1, and other structural forms should also fall within the protection scope of the present application.

[0063] Preferably, the first opening 5 is provided on the peripheral wall of the excitation channel 3 on the side away from the emission part 2, and the second opening 6 is provided on the peripheral wall of the emission channel 4 on the side away from the excitation part 1, so as to facilitate the installation, removal and movement of the fluorescence excitation component 130 and the fluorescence emission component 140, and thus improve the detection efficiency.

[0064] Specifically, the upper left side wall of the excitation part 1 is provided with an opening to form the first opening 5, and the right side wall of the emission part 2 is provided with an opening to form the second opening 6. By means of the excitation part 1 and the emission part 2 provided with openings, the weight of the fluorescence detection part 110 is reduced, so as to realize the light weight of the fluorescence detection part 110.

[0065] Further, a first cover body 7 is covered on the first opening 5, and a second cover body 8 is covered on the second opening 6. The first opening 5 is closed and opened by the first cover body 7, and the second opening 6 is closed and opened by the second cover body 8, so as to realize the closing and opening of the fluorescence detection part 110, and thus reduce the dust entering the excitation channel 3 and the emission channel 4.

[0066] Preferably, a first mounting plate 11 is provided at the exit end of the excitation channel 3. An exit port is formed on the first mounting plate 11, and the center of the exit port is located on the central axis of the excitation channel 3; a second mounting plate 12 is provided at the entrance end of the emission channel 4. An entrance port is formed on the second mounting plate 12, and the center of the entrance port is located on the central axis of the emission channel 4; a plugging groove is formed on the side of the first mounting plate 11 and the second mounting plate 12 facing away from each other. The first cover body 7 and the second cover body 8 are both formed with plugging protrusions that are plugged and matched with the plugging groove.

[0067] In some embodiments, both the fluorescence excitation component 130 and the fluorescence emission component 140 include a plurality of optical elements. A plurality of first plugging grooves 9 that are axially spaced and communicated with the first opening 5 are formed on the inner peripheral wall of the excitation channel 3, and a plurality of second plugging grooves 10 that are axially spaced and communicated with the second opening 6 are formed on the inner peripheral wall of the emission channel 4. The plurality of optical elements are installed through the first plugging grooves 9 and the second plugging grooves 10, so as to facilitate the installation and removal of the optical elements, and thus improve the detection efficiency.

[0068] Preferably, the notch of the first insertion slot 9 faces perpendicular to the central axis of the excitation channel 3, so that the plane where each optical element of the fluorescence excitation assembly 130 is located is perpendicular to the central axis of the excitation channel 3. At the same time, ensure that the size of the first insertion slot 9 matches the size of each optical element of the fluorescence excitation assembly 130, so that the centers of each optical element of the fluorescence excitation assembly 130 are located at the center of the excitation channel 3 after being inserted into the first insertion slot 9, and further make the optical path of the fluorescence excitation assembly 130 coincide with the central axis of the excitation channel 3; the notch of the second insertion slot 10 faces perpendicular to the central axis of the emission channel 4, so that the plane where each optical element of the fluorescence emission assembly 140 is located is perpendicular to the central axis of the emission channel 4. At the same time, ensure that the size of the second insertion slot 10 matches the size of each optical element of the fluorescence emission assembly 140, so that the centers of each optical element of the fluorescence emission assembly 140 are located at the center of the emission channel 4 after being inserted into the second insertion slot 10, and further make the optical path of the fluorescence emission assembly 140 coincide with the central axis of the emission channel 4.

[0069] In some embodiments, the fluorescence detection member 110 includes a plurality of excitation portions 1 and a plurality of emission portions 2 arranged in one-to-one correspondence with the plurality of excitation portions 1. The plurality of excitation portions 1 are arranged in sequence along the radial direction of the excitation channel 3, and the plurality of emission portions 2 are arranged in sequence along the radial direction of the emission channel 4. Adjacent excitation portions 1 are separated from each other and are independent of each other, and adjacent emission portions 2 are separated from each other and are independent of each other.

[0070] Specifically, the plurality of excitation portions 1 are arranged in sequence and connected in the front-rear direction, and the plurality of emission portions 2 are arranged in sequence and connected in the front-rear direction. Each excitation portion 1 and emission portion 2 are correspondingly arranged and are provided with a set of fluorescence excitation assembly 130 and fluorescence emission assembly 140, so as to realize the simultaneous detection of a plurality of samples to be tested, and further improve the detection efficiency.

[0071] Preferably, different types of fluorescence excitation assemblies 130 can be installed in the plurality of excitation portions 1, and different types of fluorescence emission assemblies 140 can be installed in the plurality of emission portions. Different samples to be tested are detected by different types of fluorescence excitation assemblies 130 and fluorescence emission assemblies 140, so as to reduce the time for installing and removing the optical excitation assembly and the fluorescence emission assembly 140, and further improve the detection efficiency.

[0072] As shown in the figure, a specific embodiment of the present application further provides a fluorescence detection device 100, including a fluorescence detection member 110, a fluorescence excitation assembly 130 and a fluorescence emission assembly 140. Among them, the fluorescence excitation assembly 130 is installed in the excitation channel 3; the fluorescence emission assembly 140 is installed in the emission channel 4. Since the fluorescence detection device 100 adopts all embodiments of the fluorescence detection member 110, the fluorescence detection device 100 has all the beneficial effects of the fluorescence detection member 110.

[0073] In some embodiments, the fluorescence detection device 100 further includes a detection carrier 120 for loading a sample to be detected. In the usage state of the fluorescence detection device 100, the detection carrier 120 is located in the detection space 13 between the excitation unit 1 and the emission unit 2 and is arranged at an interval from the fluorescence detection member 110.

[0074] Preferably, the central axis of the emission channel 4 is perpendicular to the detection surface of the detection carrier 120. The detection carrier 120 is an integrated cartridge, and the bottom surface of the integrated cartridge is the detection surface. By setting the detection surface perpendicular to the central axis of the emission channel 4, the reflected light emitted by the sample to be detected can enter the emission channel 4 as much as possible to be detected by the fluorescence emission component 140, thereby improving the detection sensitivity.

[0075] Specifically, the integrated cartridge includes a plurality of reaction zones arranged at intervals in the front-rear direction. The plurality of reaction zones are respectively used for loading a plurality of samples to be detected. The plurality of reaction zones are respectively arranged corresponding to multiple groups of excitation channels 3 and emission channels 4. The fluorescence excitation component 130 and the fluorescence emission component 140 in each group of excitation channels 3 and emission channels 4 are respectively used to detect the samples to be detected in different reaction zones, thereby improving the detection efficiency of the fluorescence detection device 100.

[0076] Further, when multiple reaction zones are loaded with multiple identical samples to be detected, the fluorescence excitation component 130 and the fluorescence emission component 140 in each group of excitation channels 3 and emission channels 4 are the same. Thus, it is not necessary to move the fluorescence detection device 100 to detect multiple identical samples to be detected, thereby improving the detection efficiency of the fluorescence detection device 100 for multiple identical samples to be detected; when multiple reaction zones are loaded with multiple different samples to be detected, the fluorescence excitation component 130 and the fluorescence emission component 140 in each group of excitation channels 3 and emission channels 4 are all different. Thus, it is not necessary to install and disassemble different fluorescence excitation components 130 and fluorescence emission components 140 to detect different samples to be detected, thereby improving the detection efficiency of the fluorescence detection device 100 for multiple different samples to be detected.

[0077] Those skilled in the art can understand that the present application is not limited to the above integrated cartridge, and can also be a split cartridge. One split cartridge contains one sample to be detected, and each split cartridge is arranged corresponding to the fluorescence excitation component 130 and the fluorescence emission component 140 in each group of excitation channels 3 and emission channels 4.

[0078] In some embodiments, the fluorescence excitation component 130 includes an excitation light source 131, an excitation collimating lens 132, an excitation filter 133, and an excitation focusing lens 134 that are arranged in sequence and have their optical paths connected. The centers of the excitation light source 131, the excitation collimating lens 132, the excitation filter 133, and the excitation focusing lens 134 are all located on the central axis of the excitation channel 3; the fluorescence emission component 140 includes an emission collimating lens 141, an emission filter 142, an emission focusing lens 143, and a detector 144 that are arranged in sequence and have their optical paths connected. The centers of the emission collimating lens 141, the emission filter 142, the emission focusing lens 143, and the detector 144 are all located on the central axis of the emission channel 4.

[0079] Specifically, the excitation light source 131 is arranged at the incident end of the excitation channel 3. Three first insertion slots 9 are formed on the inner peripheral wall of the excitation channel 3. The excitation collimating lens 132, the excitation filter 133, and the excitation focusing lens 134 are respectively inserted and matched with the three first insertion slots 9; the detector 144 is arranged at the detection end of the emission channel 4. Three second insertion slots 10 are formed on the inner peripheral wall of the emission channel 4. The emission collimating lens 141, the emission filter 142, and the emission focusing lens 143 are respectively inserted and matched with the three second insertion slots 10.

[0080] Furthermore, the excitation collimating lens 132, the excitation filter 133, the excitation focusing lens 134, the emission collimating lens 141, the emission filter 142, and the emission focusing lens 143 are injection-molded non-circular parts. The injection-molded non-circular parts are provided with assembly ears, and the assembly ears are inserted and matched with the slot walls of the first insertion slots 9 or the second insertion slots 10, so as to facilitate the installation and fixation of each optical element.

[0081] Among them, the excitation light source 131 can be a laser, an LED lamp, a mercury lamp, a halogen lamp, a xenon lamp, etc.; the detector 144 can be a photodiode, an avalanche diode, a photomultiplier tube, an imaging device, etc.; the structures and functions of the excitation collimating lens 132, the excitation filter 133, the excitation focusing lens 134, the emission collimating lens 141, the emission filter 142, and the emission focusing lens 143 are well-known to those skilled in the art and do not belong to the core improvement part of this application, so they will not be elaborated here.

[0082] As Figure 9 shown, the excitation optical path of the fluorescence excitation component 130 intersects with the emission optical path of the fluorescence emission component 140 in the detection space 13, and the detection carrier 120 is located in the detection space 13.

[0083] Specifically, an LED lamp is used as the excitation light source 131, and an LED driver board is provided at the bottom of the LED lamp; the excitation collimating lens 132 is an LED collimating lens, and the excitation focusing lens 134 is an LED focusing lens, which are specifically used for the optical action of the LED lamp; a photodiode is used as the detector 144, and a detector driver board is provided at the bottom of the detector 144.

[0084] Further, the excitation light emitted by the excitation light source 131 sequentially passes through the excitation collimating lens 132, the excitation filter 133, and the excitation focusing lens 134 and then irradiates the reaction solution in the detection carrier 120. After the probe or dye in the reaction solution is excited, emission light is emitted. The emission light sequentially passes through the emission collimating lens 141, the emission filter 142, and the emission focusing lens 143 and is then detected by the detector 144.

[0085] In the description of the present application, it should be understood that the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise specifically defined.

[0086] In the present application, unless otherwise clearly defined and limited, the terms "mounted", "connected", "connected", "fixed", etc. should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection, an electrical connection, or communicable with each other; it may be directly connected, or indirectly connected through an intermediate medium, and may be the internal communication of two components or the interaction relationship between two components, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0087] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples", etc. means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0088] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present application. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present application.

Claims

1. A fluorescence detection element, characterized in that: include: An excitation section (1) is provided with an excitation channel (3) therein, and a peripheral wall of the excitation channel (3) is provided with a first opening (5) for installing a fluorescence excitation component (130); and The emission part (2) is connected to the excitation part (1) and has an emission channel (4) therein; the emission channel (4) is arranged separately from the excitation channel (3); the central axis of the emission channel (4) intersects with the central axis of the excitation channel (3); and the peripheral wall of the emission channel (4) is provided with a second opening (6) for installing a fluorescent emission component (140).

2. The fluorescence detection element according to claim 1, characterized in that: The inner peripheral wall of the excitation channel (3) is formed with a plurality of first plug-in slots (9) spaced apart in the axial direction and connected to the first opening (5), and the inner peripheral wall of the emission channel (4) is formed with a plurality of second plug-in slots (10) spaced apart in the axial direction and connected to the second opening (6).

3. The fluorescence detection element according to claim 2, characterized in that: The notch of the first plug-in slot (9) is oriented perpendicularly to the central axis of the excitation channel (3), and the notch of the second plug-in slot (10) is oriented perpendicularly to the central axis of the emission channel (4).

4. The fluorescence detection element according to claim 2, characterized in that: The fluorescence detection element (110) comprises a plurality of the excitation parts (1) and a plurality of the emission parts (2) arranged one by one with the plurality of the excitation parts (1); the plurality of the excitation parts (1) are arranged in sequence along the radial direction of the excitation channel (3); and the plurality of the emission parts (2) are arranged in sequence along the radial direction of the emission channel (4).

5. The fluorescence detection element according to claim 4, characterized in that: The first opening (5) is arranged on the peripheral wall of the excitation channel (3) away from the emission portion (2), and the second opening (6) is arranged on the peripheral wall of the emission channel (4) away from the excitation portion (1); and / or, The first opening (5) is covered with a first cover body (7), and the second opening (6) is covered with a second cover body (8).

6. The fluorescence detection element according to claim 1, characterized in that: The excitation part (1) and the emission part (2) are integrally formed; and / or, The excitation part (1) and the emission part (2) are connected to form a V-shaped structure.

7. The fluorescence detection element according to claim 1, characterized in that: A detection space (13) is formed at the intersection of the central axis of the emission channel (4) and the central axis of the excitation channel (3), and the detection space (13) is used to accommodate a detection carrier (120) containing a sample to be detected.

8. A fluorescence detection device, characterized in that: include: The fluorescence detection element (110) according to any one of claims 1 to 7; A fluorescence excitation component (130) installed in the excitation channel (3); and A fluorescent emission component (140) is installed in the emission channel (4).

9. The fluorescence detection device according to claim 8, characterized in that: The fluorescence detection device (100) further comprises a detection carrier (120) for carrying a sample to be detected. When the fluorescence detection device (100) is in use, the detection carrier (120) is located in the detection space (13) between the excitation part (1) and the emission part (2) and is arranged at a distance from the fluorescence detection element (110).

10. The fluorescence detection device according to claim 8, characterized in that: The fluorescence excitation component (130) comprises an excitation light source (131), an excitation collimating lens (132), an excitation filter (133) and an excitation focusing lens (134) which are arranged in sequence and are optically connected, and the centers of the excitation light source (131), the excitation collimating lens (132), the excitation filter (133) and the excitation focusing lens (134) are all located on the central axis of the excitation channel (3); and / or, The fluorescence emission component (140) comprises an emission collimating lens (141), an emission filter (142), an emission focusing lens (143) and a detector (144) which are arranged in sequence and optically connected, and the centers of the emission collimating lens (141), the emission filter (142), the emission focusing lens (143) and the detector (144) are all located on the central axis of the emission channel (4).

Images