Microbial strain incubator

Abstract

The utility model discloses a microbial strain culture device which comprises a culture device main body, at least one group of culture parts are arranged in the culture device main body, at least one group of heating parts are arranged outside the culture device main body, and the culture parts and the heating parts are arranged at intervals. A gas collection disperser is arranged at the upper end of the incubator main body, and an aeration device is arranged at the bottom of the incubator main body; the culture part comprises a culture framework and a woven mesh arranged on the culture framework, and spherical filler is arranged on the woven mesh. According to the culture device, the multiple steel frameworks are arranged on the inner wall of the culture device main body, and the elastic filler is connected in series in the gaps of the meshes through the woven ropes, so that the distance between the elastic filler is effectively controlled, and the elastic filler is prevented from falling off; and meanwhile, the elastic filler is prevented from being too dense, and the growth environments of the respective biological membranes can be relatively independent, so that the treatment effect is improved, the service life of the filler is prolonged, and the maintenance cost is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of sewage treatment, in particular to a microbial strain culture device. Background Art

[0002] The biological method uses microorganisms to convert organic pollutants into inorganic substances to achieve water purification. In this process, microorganisms need to attach to a carrier to form a biofilm. Elastic fillers are widely used in sewage treatment due to their good elasticity and wear resistance. The biofilm formation method directly affects the effect of the biofilm. Traditional methods include natural biofilm formation and artificial biofilm formation. Natural biofilm formation relies on environmental microorganisms. The process is simple but time-consuming and the effect is unstable. Artificial biofilm formation quickly forms a biofilm by adding microorganisms, but the operation is cumbersome and costly. Existing methods often cannot evenly control the spacing between elastic fillers, resulting in different biofilm thicknesses, which in turn affects the treatment efficiency. At the same time, the complexity and high cost of the operation also limit its large-scale application. Utility Model Content

[0003] The purpose of the utility model is to provide a microbial strain culture device to solve the problems raised in the above background technology.

[0004] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0005] A microbial strain incubator comprises an incubator body, wherein at least one incubator part is provided inside the incubator body, and at least one heating part is provided outside the incubator body, wherein the incubator part and the heating part are arranged at intervals, a gas collecting and dispersing device is provided at the upper end of the incubator body, and an aeration device is provided at the bottom of the incubator body;

[0006] The culture part comprises a culture frame and a woven net arranged on the culture frame, and a spherical filler is arranged on the woven net.

[0007] As a further solution of the utility model: the culture vessel body is a cylindrical tube structure, the lower part of the culture vessel body is provided with a water inlet, the upper part of the culture vessel body is provided with a water outlet, and the water outlet is provided with a water outlet valve.

[0008] As a further solution of the utility model: the gas collecting and dispersing device is provided with a gas dispersing hole.

[0009] As a further solution of the utility model: the culture parts are provided with two groups, and the vertical distance between the two groups of culture parts is not less than half of the height of the internal space of the culture device body.

[0010] As a further solution of the utility model: the two groups of culture parts include a first culture skeleton and a second culture skeleton, the first culture skeleton and the second culture skeleton are both provided with a woven mesh, the woven mesh is provided with a plurality of nodes, and the spherical fillers are fixedly connected to the nodes.

[0011] As a further solution of the utility model: the heating part is provided with three groups and are respectively the first heating wire, the second heating wire and the third heating wire. The first heating wire, the second heating wire and the third heating wire are arranged at intervals with the first culture skeleton and the second culture skeleton. The first heating wire, the second heating wire and the third heating wire are connected to the controller through signal wires.

[0012] As a further solution of the utility model: the spherical filler includes a shell and a filler arranged in the shell, the shells are connected by a snap buckle, the snap buckle includes a hidden buckle, and an upper half serrated structure and a lower half serrated structure are provided on both sides of the hidden buckle.

[0013] As a further solution of the utility model: the aeration device includes a microporous aeration disk and an aeration hose, and the microporous aeration disk and the aeration hose are fixed to the bottom of the culture vessel body through an iron bracket.

[0014] As a further solution of the utility model: the aeration hose is connected to form a ring structure through a hose connector, and aeration holes are provided on the aeration hose.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] 1. The present application effectively controls the distance between the elastic fillers by arranging multiple steel frames on the inner wall of the culture body and connecting the elastic fillers in series in the grid gaps with braided ropes, thereby ensuring uniform thickness of the biofilm and avoiding over-density of the elastic fillers, thereby ensuring that the growth environment of each biofilm can be relatively independent, which not only improves the treatment effect, but also prolongs the service life of the fillers and reduces maintenance costs;

[0017] 2. The biofilm formation operation of this application is simple and easy, and does not require the artificial addition of microbial strains, which significantly reduces the difficulty and cost of operation and is very suitable for large-scale applications. At the same time, since the advantages of elastic fillers such as elasticity and wear resistance are fully utilized, the application range is wider;

[0018] 3. The present application sets electric heating devices at the upper, middle and lower parts of the incubator, and achieves temperature control balance of the entire system through the regulation of the temperature control system;

[0019] 4. The present application adopts a ring-mounted liftable aeration hose at the bottom, and the tiny aeration holes on the tube wall can greatly improve the aeration efficiency during the culture process and enhance the comprehensive culture efficiency level of bacteria. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the structure of the culture device in this embodiment;

[0021] Figure 2 This is a schematic diagram of the skeleton structure of this embodiment;

[0022] Figure 3 This is a schematic diagram of the structure of the aeration hose in this embodiment;

[0023] Figure 4 This is a schematic diagram of the spherical filler structure of this embodiment;

[0024] Figure 5 It is an enlarged schematic diagram of the local structure of the buckle in this embodiment.

[0025] In the figure: 1-culture vessel body, 2-gas collection disperser, 3-gas escape hole, 4-water outlet, 5-water outlet valve, 6-first culture skeleton, 7-first heating wire, 8-woven mesh, 9-spherical filler, 10-second heating wire, 11-controller, 12-signal wire, 13-second culture skeleton, 14-water inlet, 15-microporous aeration disk, 16-aeration hose, 17-iron bracket, 18-node, 19-hose connector, 20-aeration hole, 21-filler, 22-buckle, 22A-hidden buckle, 22B-upper half serrated structure, 22C-lower half serrated structure, 23-third heating wire. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] See also Figure 1-5 In an embodiment of the utility model, a microbial strain culture device includes a culture device body 1, which is a cylindrical tube structure. A water inlet 14 is provided at the lower part of the culture device body 1, a water outlet 4 is provided at the upper part of the culture device body 1, and a water outlet valve 5 is provided on the water outlet 4. A gas collecting and dispersing device 2 is provided at the upper end of the culture device body 1, and an aeration device is provided at the bottom of the culture device body 1. A gas dispersing hole 3 is provided on the gas collecting and dispersing device 2 to facilitate gas overflow; the horizontal area of ​​the gas dispersing hole 3 should be greater than or equal to two-thirds of the horizontal surface area of ​​the gas collecting and dispersing device 2.

[0028] The aeration device includes a microporous aeration plate 15 and an aeration hose 16. The microporous aeration plate 15 and the aeration hose 16 are fixed to the bottom of the culture vessel body 1 through an iron bracket 17. The aeration hose 16 is connected to form a ring structure through a hose connector 19. The aeration hose 16 is provided with aeration holes 20. The aeration hose 16 and the microporous aeration plate 15 are connected to the controller 11 through a signal wire 12.

[0029] At least one group of culture parts is provided in the culture vessel main body 1, and at least one group of heating parts is provided outside the culture vessel main body 1. The culture parts and the heating parts are arranged at intervals. In the present embodiment, two groups of culture parts are provided, and the vertical distance between the two groups of culture parts is not less than half of the height of the internal space of the culture vessel main body 1. The two groups of culture parts include a first culture skeleton 6 and a second culture skeleton 13. The heating parts are provided with three groups and are respectively a first heating wire 7, a second heating wire 10, and a third heating wire 23. The first heating wire 7, the second heating wire 10, and the third heating wire 23 are arranged at intervals from the first culture skeleton 6 and the second culture skeleton 13. The first heating wire 7, the second heating wire 10, and the third heating wire 23 are connected to the controller 11 through a signal wire 12. The culture part includes a culture skeleton and a woven mesh 8 arranged on the culture skeleton, the woven mesh 8 is provided with a spherical filler 9, the spherical filler 9 includes a shell and a filler 21 arranged in the shell, the shells are connected by a buckle 22, the buckle 22 includes a hidden buckle 22A, an upper half serrated structure 22B and a lower half serrated structure 22C are arranged on both sides of the hidden buckle 22A, the upper half serrated structure 22B and the lower half serrated structure 22C are connected in the middle by the hidden buckle 22A, the first culture skeleton 6 and the second culture skeleton 13 are both provided with a woven mesh 8, and a plurality of nodes 18 are provided on the woven mesh 8, the spherical filler 9 is fixedly connected to the node 18, the front, back, left and right nodes 18 are spaced at least 0.2m apart, the inner diameter of the spherical filler 9 is 0.1m, and the outer diameter of the filler 21 is 0.07m.

[0030] In summary, this embodiment includes the following unit structures:

[0031] Inlet and outlet unit structure: The water inlet of the microbial culture vessel is arranged on the lower part of the left wall to connect the water inlet facilities. The water inlet flow rate and time can be set according to the actual working condition analysis. The water outlet is arranged on the upper part of the right wall to connect the water outlet facilities. By connecting the valve and the control device, the output flow rate and drainage time are controlled, and finally the water level in the culture vessel is controlled.

[0032] Aeration unit: The aeration unit is installed at the bottom of the microbial culture vessel, which is divided into two parts: 1) A circular aeration hose is installed at the bottom of the structure, supported by iron brackets at both ends; 2) Multiple microporous aeration disks are arranged in the middle of the circular aeration hose to improve the overall aeration level. The dissolved oxygen level during the culture process is precisely controlled by an external control unit.

[0033] Heating unit: Three heating wires (first heating wire, second heating wire and third heating wire) are installed on the upper, middle and lower side walls of the microbial culture chamber to ensure uniform heating in the chamber, which is suitable for microbial culture that requires temperature control. The heating wires are connected to the automatic control system through wires to ensure that the temperature of the chamber is within a controllable range.

[0034] Gas treatment system: A gas collection disperser is installed on the top of the microbial culture chamber to effectively discharge the gas generated during the culture process through the gas escape holes to prevent gas accumulation from affecting the culture environment.

[0035] When using this embodiment, the incubator is first installed. After the installation is completed, the device system is adjusted, including stability adjustment and aeration system adjustment. Then the culture solution is prepared, and 5L of anaerobic ammonia oxidation seed bacteria and 5L of nutrient solution are used to prepare the mixed culture solution, and the added nutrients include ammonium chloride, sodium nitrite, sodium acetate, and ammonium bicarbonate. The dissolved oxygen (DO) of the reaction is controlled to be ≤0.5mg / L, the water temperature is maintained between 30℃ and 40℃, and the pH value of the reactor is adjusted between 7.5 and 8.5 using dilute hydrochloric acid and NaOH.

[0036] The water inlet at the bottom of the side wall is opened, and the mixed reaction liquid is injected with one reaction cycle as the time node. The reaction liquid is evenly mixed and stirred in the whole device through the aeration device at the bottom. After sufficient mixing and reaction, part of the liquid flows out from the water outlet 4 at the top of the side wall of the cylindrical reactor to ensure that the water outlet is unobstructed. If necessary, adjust the residence time of the mixed culture liquid to maintain the water level in the culture vessel at three quarters of the total height.

[0037] The controller 11 automatically adjusts the working state of the heating wire and the aeration hose according to the preset parameters to maintain the best culture environment. Microorganisms grow and reproduce on the surface of the spherical filler 9. During the culture period, part of the culture solution is regularly discharged through the water outlet 4 and fresh culture solution is added through the water inlet 14 to maintain the nutritional balance of the entire system. The operator needs to regularly check the operating status of the intelligent control device and make adjustments according to the actual situation, such as increasing the aeration volume or adjusting the pH value and temperature.

[0038] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0039] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A microbial strain incubator, comprising an incubator body (1), characterized in that: The culture vessel body (1) is provided with at least one culture section inside, and at least one heating section outside the culture vessel body (1), wherein the culture section and the heating section are arranged at intervals, and a gas collecting and dispersing device (2) is provided at the upper end of the culture vessel body (1), and an aeration device is provided at the bottom of the culture vessel body (1); The culture part comprises a culture frame and a woven net (8) arranged on the culture frame, and a spherical filler (9) is arranged on the woven net (8).

2. A microbial culture device according to claim 1, characterized in that: The culture vessel body (1) is a cylindrical structure. The lower part of the culture vessel body (1) is provided with a water inlet (14). The upper part of the culture vessel body (1) is provided with a water outlet (4). The water outlet (4) is provided with a water outlet valve (5).

3. A microbial culture device according to claim 1, characterized in that: The gas collecting and dispersing device (2) is provided with gas dispersing holes (3).

4. A microbial culture device according to claim 1, characterized in that: The culture parts are provided in two groups, and the vertical distance between the two groups of culture parts is not less than half the height of the internal space of the culture device body (1).

5. A microbial culture device according to claim 4, characterized in that: The two groups of culture parts include a first culture skeleton (6) and a second culture skeleton (13). The first culture skeleton (6) and the second culture skeleton (13) are both provided with a woven mesh (8). The woven mesh (8) is provided with a plurality of nodes (18). The spherical fillers (9) are fixedly connected to the nodes (18).

6. A microbial culture device according to claim 5, characterized in that: The heating part is provided with three groups, namely a first heating wire (7), a second heating wire (10), and a third heating wire (23); the first heating wire (7), the second heating wire (10), and the third heating wire (23) are arranged at intervals from the first culture skeleton (6) and the second culture skeleton (13); the first heating wire (7), the second heating wire (10), and the third heating wire (23) are connected to a controller (11) via a signal wire (12).

7. A microbial culture device according to claim 1, characterized in that: The spherical filler (9) comprises a shell and a filler (21) arranged in the shell. The shells are connected by a buckle (22). The buckle (22) comprises a concealed buckle (22A). An upper sawtooth structure (22B) and a lower sawtooth structure (22C) are provided on both sides of the concealed buckle (22A).

8. A microbial culture device according to claim 1, characterized in that: The aeration device comprises a microporous aeration plate (15) and an aeration hose (16). The microporous aeration plate (15) and the aeration hose (16) are fixed to the bottom of the culture vessel body (1) via an iron bracket (17).

9. A microbial culture device according to claim 8, characterized in that: The aeration hose (16) is connected to form a ring structure through a hose connector (19), and an aeration hole (20) is provided on the aeration hose (16).

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