Nucleic acid extraction and purification instrument

Abstract

The utility model discloses a nucleic acid extraction and purification instrument, which relates to the technical field of biological detection equipment and is characterized by comprising an instrument main body, a containing table, a support plate and a mounting frame are arranged in the instrument main body, the support plate is positioned between the containing table and the mounting frame, and a plurality of deep hole plates are detachably connected onto the containing table. A first control frame and a second control frame which are located above the containing table are slidably connected to the support plate, a plurality of magnetic bars and a plurality of columns of magnetic bar sleeves are detachably connected to the first control frame and the second control frame respectively, the mounting frame is fixedly connected to the support plate and horizontally slidably connected into the instrument body through a first sliding rail, and the first sliding rail is located below the mounting frame. A driver I and a driver II for respectively driving the control frame I and the control frame II are arranged in the mounting frame; and the driver I is positioned above the driver II. Through the structural arrangement, the internal structure of the nucleic acid extraction and purification instrument is compact and reasonable in arrangement, meanwhile, a large number of samples can be operated, and the extraction efficiency is relatively high.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection equipment, and more specifically, to a nucleic acid extraction and purification instrument. Background Art

[0002] Nucleic acid extraction technology is an important method to achieve precision medicine in the biological and medical fields. In order to avoid sample contamination and save labor costs, traditional manual experimental extraction has gradually been replaced by automated nucleic acid extraction, that is, automated nucleic acid extraction can be achieved through pre-set nucleic acid extraction programs.

[0003] In the prior art, the nucleic acid extractor adopts magnetic bead separation technology, and the adsorption and transfer of magnetic beads are achieved through the coordinated movement of the magnetic rod and the magnetic rod sleeve. At the same time, the liquid is repeatedly stirred by the magnetic rod sleeve to fully mix the reaction liquid. Then, after the steps of sample lysis, nucleic acid adsorption to magnetic beads, magnetic bead washing and nucleic acid elution, purified nucleic acid is finally obtained, which can be used for the extraction and purification of nucleic acid in clinical samples. However, the current nucleic acid extractor can extract at most two single-channel nucleic acids at a time, and the number of nucleic acids extracted at one time is too small, resulting in slow nucleic acid extraction and low efficiency. In addition, the current nucleic acid extraction instruments are relatively large in size and are not convenient for transportation and carrying.

[0004] Therefore, it is necessary to provide a nucleic acid extraction and purification instrument to solve the above problems. Utility Model Content

[0005] The purpose of the utility model is to provide a nucleic acid extraction and purification instrument to solve the problems raised in the above background technology.

[0006] The above technical objectives of the utility model are achieved through the following technical solutions:

[0007] A nucleic acid extraction and purification instrument comprises an instrument body, wherein a containing table, a support plate and a mounting frame are arranged in the instrument body, wherein the support plate is located between the containing table and the mounting frame, and a plurality of deep-well plates are detachably connected to the containing table.

[0008] The support plate is slidably connected to a control frame 1 and a control frame 2 located above the accommodating platform, and the control frame 1 and the control frame 2 are respectively detachably connected to a plurality of magnetic bars and a plurality of rows of magnetic bar sleeves.

[0009] The installation frame is fixedly connected to the bracket plate, and the installation frame is horizontally slidably connected to the instrument body through a slide rail 1, and the slide rail 1 is located below the installation frame. Driver 1 and driver 2 are respectively provided in the installation frame to drive the control frame 1 and the control frame 2, and the driver 1 and the driver 2 are both located above the slide rail 1, and the driver 1 is located above the driver 2.

[0010] The technical solution of the utility model is further configured as follows: the driver 1 and the driver 2 are both horizontally placed motors 1, the output shafts of the two motors 1 respectively pass through the two sides of the installation frame, rollers 1 are provided on both sides of the installation frame, the output shafts of the two motors 1 are connected to the corresponding rollers 1 through a transmission belt 1, the control frame 1 and the control frame 2 are respectively connected to the two transmission belts 1 through connecting plates, and two slide grooves 1 are provided on the bracket plate for accommodating the two connecting plates respectively.

[0011] The technical solution of the utility model is further configured as follows: the bracket plate is provided with a longitudinally arranged slide rail 2, the two connecting plates are slidably connected to the slide rail 2 at one end away from the transmission belt 1 connected to each of them, and the control frame 1 and the control frame 2 are detachably connected to the corresponding connecting plates of the two.

[0012] The technical solution of the utility model is further configured as follows: rollers 2 are provided at both ends of the slide rail 1, one of the rollers 2 is driven by a motor 2, the two rollers 2 are connected by a transmission belt 2, and the bottom end of the mounting frame is fixedly connected to the transmission belt 2.

[0013] The technical solution of the utility model is further configured as follows: the accommodating table is provided with limit strips alternately arranged with the deep-hole plates, the bottom end of the deep-hole plates is provided with a convex portion, and the limit strip is provided with an obstruction member extending from the inside thereof on one side facing the deep-hole plates, and the obstruction member is connected to the limit strip by a spring and abuts against the top end of the convex portion.

[0014] The technical solution of the utility model is further configured as follows: a plurality of heating strips are arranged between the adjacent limiting strips, the heating strips are located below the deep-hole plates, and a plurality of heating grooves are opened on the top surfaces of the heating strips.

[0015] The technical solution of the utility model is further configured as follows: a plurality of slide grooves 2 are opened at the bottom end of the control frame 2, a plurality of through grooves are opened at the top end of the control frame 2, the through grooves are located above the slide groove 2 and connected thereto, the cross-sectional shape of the slide groove 2 is T-shaped, and the magnetic rod sleeve is slidably and detachably connected in the slide groove 2.

[0016] The technical solution of the utility model is further configured as follows: a gripping portion is provided at one end of the magnetic rod sleeve in the length direction, and the width of the gripping portion is greater than the width of the second slide groove.

[0017] The technical solution of the utility model is further configured as follows: an ultraviolet lamp located above the control frame 1 is provided on the inner top wall of the instrument body.

[0018] The technical solution of the utility model is further configured as follows: a cooling fan is provided on the inner wall of the instrument body, and the cooling fan is located at a side of the installation frame away from the bracket plate.

[0019] Due to the adoption of the above technical solution, the utility model has achieved the following technical progress compared with the prior art:

[0020] Through the arrangement of the structure, the driver 1, the driver 2 and the slide rail 1 are located at different heights in the same area, so that the driving part in the instrument body is compactly arranged together, reducing the volume of the instrument body and facilitating its carrying. The installation frame is convenient for accommodating the driver 1 and the driver 2, while the driver 1 and the driver 2 can drive the two connecting plates to move from two directions respectively, thereby ensuring that the control frame 1 and the control frame 2 can work stably, and the arrangement of the slide rail 2 makes the movement of the control frame 1 and the control frame 2 more stable.

[0021] The arrangement of the deep-well plate, magnetic rod and magnetic rod sleeve enables nucleic acid extraction from a large number of samples, significantly improving the speed and efficiency of nucleic acid extraction; the heating bar located between adjacent limit bars can utilize the heating groove on its top surface to cooperate with the bottom end of the deep-well plate, making it less likely to shift or misalign the position, and is beneficial to improving the quality and efficiency of nucleic acid extraction; the holding portion on the magnetic rod sleeve makes it easy to install the magnetic rod sleeve on the control frame 2, and when the holding portion abuts against the outer wall of the control frame 2, it can be confirmed that the magnetic rod sleeve is installed in place, making it less likely to have errors, thereby ensuring that the magnetic rod sleeve and the magnetic rod are accurately matched. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The structure of the instrument body in the utility model is shown in FIG. Figure 1 ;

[0023] Figure 2 The structure of the instrument body in the utility model is shown in FIG. Figure 2 ;

[0024] Figure 3 The internal structure of the utility model is shown in FIG. Figure 1 ;

[0025] Figure 4 The internal structure of the utility model is shown in FIG. Figure 2 ;

[0026] Figure 5 The internal structure of the utility model is shown in FIG. Figure 3 ;

[0027] Figure 6 The internal structure of the utility model is shown in FIG. Figure 4 ;

[0028] Figure 7It is a structural schematic diagram of the medium and deep hole plate of the utility model.

[0029] In the figure: 1. Instrument body; 2. Accommodating table; 3. Bracket plate; 4. Mounting frame; 5. Deep hole plate; 6. Control frame 1; 7. Control frame 2; 8. Magnetic rod; 9. Magnetic rod sleeve; 10. Slide rail 1; 11. Driver 1; 12. Driver 2; 13. Roller 1; 14. Drive belt 1; 15. Connecting plate; 16. Slide groove 1; 17. Slide rail 2; 18. Roller 2; 19. Motor 2; 20. Drive belt 2; 21. Limiting strip; 22. Protrusion; 23. Obstruction piece; 24. Heating strip; 25. Heating groove; 26. Slide groove 2; 27. Through groove; 28. Grip; 29. ​​Ultraviolet lamp; 30. Cooling fan. DETAILED DESCRIPTION

[0030] In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the specific implementation methods of the utility model are further described in detail below in conjunction with the drawings and embodiments. The following embodiments are used to illustrate the utility model but are not used to limit the scope of the utility model. Example

[0031] refer to Figures 1 to 7 As shown, the utility model provides a nucleic acid extraction and purification instrument, including an instrument body 1, wherein a accommodating table 2, a bracket plate 3 and an installation frame 4 are arranged in the instrument body 1, the bracket plate 3 is located between the accommodating table 2 and the installation frame 4, the bracket plate 3 is slidably connected with a control frame 1 6 and a control frame 2 7 located above the accommodating table 2, the control frame 1 6 and the control frame 2 7 are respectively detachably connected with a plurality of magnetic rods 8 and a plurality of rows of magnetic rod sleeves 9, the installation frame 4 is fixedly connected to the bracket plate 3, and the installation frame 4 is horizontally slidably connected to the instrument through a slide rail 10. In the instrument body 1, a slide rail 10 is located below the mounting frame 4, and a driver 11 and a driver 2 12 are provided in the mounting frame 4 for driving a control frame 1 6 and a control frame 2 7 respectively, and both the driver 11 and the driver 2 12 are located above the slide rail 10, and the driver 11 is located above the driver 2 12, and an ultraviolet lamp 29 located above the control frame 1 6 is provided on the inner top wall of the instrument body 1, and a cooling fan 30 is provided on the inner wall of the instrument body 1, and the cooling fan 30 is located on the side of the mounting frame 4 away from the bracket plate 3.

[0032] By setting the above structure, the instrument body 1 is divided into a sample side and a drive side by using the bracket plate 3. The sample side has a control frame 1 6, a control frame 2 7 and a accommodating table 2, and the drive side has a mounting frame 4, a driver 11 and a driver 2 12 located in the mounting frame 4, and a slide rail 10 located below the mounting frame 4. In this way, the structure of the drive side is relatively compact, and the height does not exceed the height of the bracket plate 3, which can avoid the situation where the drive side occupies a large space in the instrument body 1, resulting in a large volume of the instrument body 1 and inconvenient to carry. In addition, the cooling fan 30 in the instrument body 1 can dissipate heat on the drive side to prevent overheating of this area.

[0033] refer to Figures 1 to 7 As shown, driver 1 11 and driver 2 12 are both horizontally placed motors 1, and the output shafts of the two motors 1 respectively pass through the two sides of the mounting frame 4, and rollers 13 are provided on both sides of the mounting frame 4. The output shafts of the two motors 1 are connected to the corresponding rollers 13 through a transmission belt 14, and the control frame 1 6 and the control frame 2 7 are connected to the two transmission belts 14 through connecting plates 15, respectively. Two slide grooves 16 for accommodating the two connecting plates 15 are provided on the bracket plate 3, and a longitudinally arranged slide rail 2 17 is provided on the bracket plate 3. One end of the two connecting plates 15 away from the transmission belt 14 connected to each other is slidably connected to the slide rail 2 17, and the control frame 1 6 and the control frame 2 7 are detachably connected to the corresponding connecting plates 15 of the two, and rollers 2 18 are provided at both ends of the slide rail 10, one of which is driven by a motor 2 19, and the two rollers 2 18 are connected through a transmission belt 20, and the bottom end of the mounting frame 4 is fixedly connected to the transmission belt 2 20.

[0034] By setting the above structure, the installation frame 4 with a square frame structure can allow the driver 1 11 and the driver 2 12 to penetrate into it, and at the same time, support the two ends of the driver 1 11 and the driver 2 12 in the length direction to ensure the stability of the two motors 1, and the output shafts of the two motors 1 are in opposite directions so that the driver 1 11 and the driver 2 12 can control the two connecting plates 15 to move up and down from two directions respectively, thereby achieving the effect of moving the control frame 1 6 and the control frame 2 7. The existence of the slide rail 2 17 restricts both ends of the connecting plate 15 and can slide smoothly along the slide rail 2 17 without bumps or shaking. The slide rail 10 located below the installation frame 4 can use the motor 2 19 to drive the installation frame 4 to slide along its length direction. The motor 2 19 can be moved as shown in the following figure. Figure 4 The reduction mechanism shown is connected to roller 2 18, so that the position of motor 2 19 can be located on one side of slide rail 10 or at one end in the length direction, and can be adjusted according to the space conditions in the instrument body 1 to ensure a reasonable distribution of space on the drive side.

[0035] refer to Figures 1 to 7 As shown, two deep-hole plates 5 are detachably connected to the accommodating table 2, and limiting strips 21 are provided on the accommodating table 2, which are alternately arranged with the deep-hole plates 5. A convex portion 22 is provided at the bottom end of the deep-hole plate 5, and two spherical obstruction members 23 extending from the inside thereof are provided on the side of the limiting strip 21 facing the deep-hole plate 5. The obstruction members 23 are connected to the limiting strip 21 through a spring and abut against the top of the convex portion 22. One end of the spring is fixedly connected in the limiting strip 21, and the other end of the spring is fixedly connected to the obstruction member 23. A plurality of heating strips 24 are provided between adjacent limiting strips 21, and the heating strips 24 are located below the deep-hole plate 5 and below the 2nd, 6th, 8th and 12th vertical rows of holes of the deep-hole plate 5. A plurality of heating grooves 25 are provided on the top surface of the heating strip 24.

[0036] By setting the above structure, when the two deep-well plates 5 are installed on the accommodating platform 2, the protrusion 22 at the bottom end of the deep-well plate 5 will contact and squeeze the obstruction member 23 on the limiting strip 21, so that the obstruction member 23 squeezes the spring and enters the limiting strip 21. After the bottom end of the deep-well plate 5 contacts the top surface of the accommodating platform 2, the obstruction member 23 can be pushed out from the limiting strip 21 again by the thrust of the spring and abut against the top end of the protrusion 22 of the deep-well plate 5, thereby achieving the limited installation effect of the deep-well plate 5. In addition, in this process, the two ends of the heating strip 24 in the length direction can abut against the inner wall of the bottom end of the deep-well plate 5, thereby further limiting the position of the deep-well plate 5 to ensure the position accuracy between it and the magnetic rod 8 and the magnetic rod sleeve 9. The heating groove 25 on the heating strip 24 is used to match the deep-well plate 5, thereby improving the heating efficiency and the extraction efficiency of nucleic acid.

[0037] refer to Figures 1 to 7 As shown, a plurality of slide grooves 26 are provided at the bottom end of the control frame 27, and a plurality of through grooves 27 are provided at the top end of the control frame 27. The through grooves 27 are located above the slide groove 26 and are connected thereto. The cross-sectional shape of the slide groove 26 is T-shaped, and the magnetic rod sleeve 9 is slidably and detachably connected in the slide groove 26. A gripping portion 28 is provided at one end in the length direction of the magnetic rod sleeve 9, and the width of the gripping portion 28 is greater than the width of the slide groove 26.

[0038] Through the arrangement of the above structure, the magnetic rod sleeve 9 can be controlled by the holding portion 28, and its installation and disassembly are relatively convenient. When the magnetic rod sleeve 9 needs to be installed on the control frame 2 7, it only needs to be inserted into the slide groove 26 and the holding portion 28 is allowed to abut against the surface of the control frame 2 7. In this way, when the control frames 1 6 and 2 7 are working, the magnetic rod 8 can accurately enter the magnetic rod sleeve 9 to ensure the stable extraction of nucleic acid.

[0039] refer to Figures 1 to 7As shown, when nucleic acid extraction is required, the deep well plate 5 containing the sample is installed on the accommodating table 2, and then the magnetic rod sleeve 9 is installed on the control frame 2 7, the hatch of the instrument body 1 can be closed, and the instrument body 1 is powered, so that the internal driver 11, driver 2 12 and motor 2 19 are powered on and start running, and the magnetic rod 8 and the magnetic rod sleeve 9 are separated to transfer the magnetic beads between the deep well plates 5 containing specific reagents to achieve the collection, release, transfer and incubation of the magnetic beads, and high-quality nucleic acids can be extracted from samples such as saliva, swabs, dried blood spots, whole blood, serum / plasma, animal and plant tissues, etc. The magnetic rod sleeve 9 has a total of 32 channels, which can process 1 to 32 samples at a time, and in the process of nucleic acid extraction, the solution volume of the previous deep well plate 5 can be larger than the volume of the next deep well plate 5, thereby achieving the purpose of concentration.

[0040] The above is only a preferred embodiment of the present invention. The protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A nucleic acid extraction and purification instrument, comprising an instrument body (1), characterized in that: The instrument body (1) is provided with a receiving platform (2), a support plate (3) and a mounting frame (4); the support plate (3) is located between the receiving platform (2) and the mounting frame (4); a plurality of deep-hole plates (5) are detachably connected to the receiving platform (2); The support plate (3) is slidably connected to a control frame 1 (6) and a control frame 2 (7) located above the accommodating platform (2); the control frame 1 (6) and the control frame 2 (7) are respectively detachably connected to a plurality of magnetic bars (8) and a plurality of rows of magnetic bar sleeves (9); The installation frame (4) is fixedly connected to the support plate (3), and the installation frame (4) is horizontally slidably connected to the instrument body (1) via a slide rail (10), and the slide rail (10) is located below the installation frame (4). The installation frame (4) is provided with a driver (11) and a driver (12) for driving the control frame (6) and the control frame (7) respectively, and the driver (11) and the driver (12) are both located above the slide rail (10), and the driver (11) is located above the driver (12).

2. A nucleic acid extraction and purification instrument according to claim 1, characterized in that: The driver 1 (11) and the driver 2 (12) are both horizontally placed motors 1, the output shafts of the two motors 1 respectively pass through the two sides of the installation frame (4), and rollers 1 (13) are provided on both sides of the installation frame (4). The output shafts of the two motors 1 are connected to the corresponding rollers 1 (13) through a transmission belt 1 (14), the control frame 1 (6) and the control frame 2 (7) are connected to the two transmission belts 1 (14) through a connecting plate (15), and two slide grooves 1 (16) are provided on the bracket plate (3) for accommodating the two connecting plates (15).

3. A nucleic acid extraction and purification instrument according to claim 2, characterized in that: The support plate (3) is provided with a longitudinally arranged slide rail 2 (17), and one end of the two connecting plates (15) away from the transmission belt 1 (14) connected to each other is slidably connected to the slide rail 2 (17), and the control frame 1 (6) and the control frame 2 (7) are detachably connected to the corresponding connecting plates (15).

4. A nucleic acid extraction and purification instrument according to claim 1, characterized in that: Both ends of the slide rail 1 (10) are provided with rollers 2 (18), one of the rollers 2 (18) is driven by a motor 2 (19), the two rollers 2 (18) are connected by a transmission belt 2 (20), and the bottom end of the mounting frame (4) is fixedly connected to the transmission belt 2 (20).

5. A nucleic acid extraction and purification instrument according to claim 1, characterized in that: The accommodating table (2) is provided with a limiting strip (21) arranged alternately with the deep hole plate (5); a convex portion (22) is provided at the bottom end of the deep hole plate (5); a blocking member (23) extending from the inside of the limiting strip (21) is provided on a surface facing the deep hole plate (5); the blocking member (23) is connected to the limiting strip (21) via a spring and abuts against the top end of the convex portion (22).

6. A nucleic acid extraction and purification instrument according to claim 5, characterized in that: A plurality of heating strips (24) are provided between adjacent limiting strips (21), the heating strips (24) are located below the deep hole plate (5), and a plurality of heating grooves (25) are provided on the top surface of the heating strips (24).

7. A nucleic acid extraction and purification instrument according to claim 1, characterized in that: The bottom end of the control frame 2 (7) is provided with a plurality of slide grooves 2 (26), and the top end of the control frame 2 (7) is provided with a plurality of through grooves (27), wherein the through grooves (27) are located above the slide groove 2 (26) and are connected thereto, and the cross-section of the slide groove 2 (26) is T-shaped, and the magnetic rod sleeve (9) is slidably and detachably connected to the slide groove 2 (26).

8. A nucleic acid extraction and purification instrument according to claim 7, characterized in that: A gripping portion (28) is provided at one end of the magnetic rod sleeve (9) in the length direction, and the width of the gripping portion (28) is greater than the width of the second slide groove (26).

9. A nucleic acid extraction and purification instrument according to claim 1, characterized in that: An ultraviolet lamp (29) is provided on the inner top wall of the instrument body (1) and is located above the control frame 1 (6).

10. A nucleic acid extraction and purification instrument according to claim 1, characterized in that: A cooling fan (30) is provided on the inner wall of the instrument body (1), and the cooling fan (30) is located on a side of the installation frame (4) away from the bracket plate (3).

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