Short nucleic acid molecule extraction device for biological information analysis

Abstract

The utility model relates to the technical field of short nucleic acid molecules, in particular to a short nucleic acid molecule extraction device for biological information analysis, which comprises a bottom plate and first vertical plates, the upper end of the bottom plate is symmetrically and fixedly connected with the first vertical plates, and the upper ends of the first vertical plates are fixedly connected with a top plate. The rear side of the upper end of the bottom plate is fixedly connected with a supporting plate, an angle fixing device is arranged on the outer wall of the supporting plate, and according to the short nucleic acid molecule extraction device for biological information analysis, through cooperation of the auxiliary pulling device and the angle fixing device, the front end of the sliding plate and the front side surface of the supporting plate can be located on the same plane; a first motor is stopped, a straight plate is tightly attached to the surface of a sliding plate, the material box can be in an inclined state, an operator can conveniently clean the inner wall of the material box, the interior of the material box can be cleaned, residues of internal sample reagents are reduced, and the next extraction and processing effect is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of short nucleic acid molecules, in particular to a short nucleic acid molecule extraction device for biological information analysis. Background Art

[0002] Through special silicon matrix adsorption materials, DNA can be specifically adsorbed, while RNA and protein pass smoothly. Then, high salt and low pH are used to bind nucleic acids, and low salt and high pH are used to elute. Nucleic acids are separated and purified by centrifugation. When extracting short nucleic acid molecules, different extraction agents are needed to carry out targeted treatment of different short nucleic acid molecules in the sample reagents.

[0003] For example, the short nucleic acid molecule extraction device for bioinformatics analysis with the authorization announcement number "CN220335165U" can be easily separated and taken out by detachably setting the extraction tank on the rotating support structure. The sample reagent can seep out from the through grooves on the outer wall of the inner feeding box and fall into the extraction tank, and the stirring ridges formed on the inner wall of the extraction tank can be used to stir and mix the reagent inside. However, the device is not convenient for cleaning the container containing the sample reagent. After the extraction of the short nucleic acid molecules is completed, the operator needs to clean it. If it is not cleaned for a long time or is not cleaned thoroughly, the residual sample reagent will deteriorate, which will affect the new sample reagent when used next time, resulting in poor extraction effect of the device when used. Utility Model Content

[0004] The utility model aims to solve the problem that the device is inconvenient and the cleaning leads to poor extraction effect, and proposes a short nucleic acid molecule extraction device for biological information analysis.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A short nucleic acid molecule extraction device for bioinformatics analysis is designed, comprising a bottom plate and a first vertical plate, wherein the upper end of the bottom plate is symmetrically fixedly connected to the first vertical plate, the upper ends of the first vertical plates are fixedly connected to the top plate, the rear side of the upper end of the bottom plate is fixedly connected to a support plate, the outer wall of the support plate is provided with an angle fixing device, the upper end of the support plate is provided with an auxiliary pulling device, the upper end of the bottom plate is symmetrically fixedly connected to the second vertical plate, and the second vertical plates on both sides are movably connected to the protruding part of the material box through bearings.

[0007] Preferably, the angle fixing device includes a slide plate and a rubber block, the outer wall of the slide plate is slidably connected to the through hole opened on the surface of the support plate, the protruding part of the upper end of the slide plate is fixedly connected to the rubber block, the front end of the rubber block is in contact with the support plate, the outer wall of the slide plate and the support plate are respectively fixedly connected to the two ends of the spring, the rear side of the slide plate is fixedly connected to a handle, the slide plate is movably connected to the rotating rod through a pin shaft, and the rear side of the support plate is fixedly connected to a groove block.

[0008] Preferably, the top plate is fixedly connected to the first box body, the inner wall of the first box body is fixedly connected to a hydraulic cylinder, the telescopic end of the hydraulic cylinder passes through the first box body, and the lower end of the material box is fixedly connected to a straight plate.

[0009] Preferably, the telescopic end of the hydraulic cylinder is fixedly connected to a second box body, the inner wall of the second box body is fixedly connected to the first motor via a motor frame, and multiple groups of through holes are provided on the surfaces of the first box body and the second box body.

[0010] Preferably, the output shaft of the first motor is rotatably connected to the second housing via a bearing, the output shaft of the first motor passes through the second housing, the output shaft of the first motor is fixedly connected to the extraction tank via bolts, and a plurality of liquid passage grooves are provided on the surface of the extraction tank.

[0011] Preferably, the auxiliary pulling device includes a drum and a bracket, one end of the bracket on both sides is fixedly connected to the support plate, and the brackets are rotatably connected to the rotating part of the drum through bearings, one side of the bracket is fixedly connected to the second motor through a motor frame, and the output shaft of the second motor is fixedly connected to the rotating part of the drum, a pull rope is wrapped around the outer wall of the drum, one end of the pull rope is fixedly connected to the connecting block through a fixed pulley, the pulley frame of the fixed pulley is fixedly connected to the upper end of the support plate, and the connecting block is fixedly connected to the material box.

[0012] The utility model proposes a short nucleic acid molecule extraction device for bioinformatics analysis, and its beneficial effects are as follows: through the cooperation of the auxiliary pulling device and the angle fixing device, the output shaft of the second motor rotates to drive the reel to move synchronously, so that the sample reagent inside the material box can be slowly poured out and collected. At the same time, the operator pulls the slide plate through the handle to move, so that the slide plate stretches the spring, and at the same time pulls the rotating rod to a horizontal state, and releases the handle. Under the action of the spring, one end of the rotating rod can be inserted into the groove block, so that the position of the slide plate is fixed, so that the front end of the slide plate can be in the same plane with the front side surface of the support plate, so that the material box can be rotated to a state beyond the horizontal state. According to the above operation, the slide plate is quickly returned to its original position, the first motor is stopped, and the straight plate is tightly attached to the surface of the slide plate, so that the material box can be in an inclined state, which is convenient for the operator to clean the inner wall of the material box. The inside of the material box can be cleaned by spraying water or the like, so as to reduce the residual sample reagent inside and ensure the effect of the next extraction processing. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the structure of the utility model;

[0014] Figure 2 for Figure 1 A front cross-sectional view of

[0015] Figure 3 for Figure 1 A partial side sectional view of

[0016] Figure 4 for Figure 3 Schematic diagram of the middle angle fixing device in the opened state;

[0017] Figure 5 It is a three-dimensional schematic diagram of the liquid channel and the extraction tank;

[0018] Figure 6 for Figure 2 Schematic diagram of part A;

[0019] Figure 7 for Figure 3 Schematic diagram of part B.

[0020] In the figure: 1, bottom plate, 2, auxiliary pulling device, 201, second motor, 202, reel, 203, bracket, 204, fixed pulley, 205, pull rope, 206, connecting block, 3, support plate, 4, straight plate, 5, material box, 6, second vertical plate, 7, first vertical plate, 8, liquid trough, 9, angle fixing device, 901, handle, 902, slide plate, 903, rubber block, 904, groove block, 905, rotating rod, 906, spring, 10, extraction tank, 11, second box body, 12, top plate, 13, first box body, 14, hydraulic cylinder, 15, first motor. DETAILED DESCRIPTION

[0021] The utility model is further described below in conjunction with the accompanying drawings:

[0022] See attached Figure 1-7 : In this embodiment, a short nucleic acid molecule extraction device for bioinformatics analysis includes a bottom plate 1 and a first vertical plate 7, the upper end of the bottom plate 1 is symmetrically fixedly connected to the first vertical plate 7, the upper ends of the first vertical plates 7 are fixedly connected to the top plate 12, the rear side of the upper end of the bottom plate 1 is fixedly connected to a support plate 3, the outer wall of the support plate 3 is provided with an angle fixing device 9, the upper end of the support plate 3 is provided with an auxiliary pulling device 2, the upper end of the bottom plate 1 is symmetrically fixedly connected to the second vertical plate 6, the second vertical plates 6 on both sides are movably connected to the protruding part of the material box 5 through bearings, and the second vertical plates 6 on both sides can support the material box 5 to rotate;

[0023] The top plate 12 is fixedly connected to the first box body 13, and the inner wall of the first box body 13 is fixedly connected to the hydraulic cylinder 14. The hydraulic cylinder 14 can meet the work needs according to actual needs. The hydraulic cylinder 14 can drive the second box body 11 to rise and fall, and the telescopic end of the hydraulic cylinder 14 passes through the first box body 13. The lower end of the material box 5 is fixedly connected to the straight plate 4, and the telescopic end of the hydraulic cylinder 14 is fixedly connected to the second box body 11. The inner wall of the second box body 11 is fixedly connected to the first motor 15 through the motor frame. The first motor 15 can meet the work needs according to actual needs. The first motor 15 can drive the extraction tank 10 to rotate. The surfaces of the first box body 13 and the second box body 11 are provided with multiple groups of through holes, and the output shaft of the first motor 15 is rotatably connected to the second box body 11 through a bearing. The output shaft of the first motor 15 passes through the second box body 11, and the output shaft of the first motor 15 is fixedly connected to the extraction tank 10 through bolts. The extraction tank 10 can be removed by rotating and removing the two bolts on the surface of the first motor 15. The surface of the extraction tank 10 is provided with multiple groups of liquid grooves 8.

[0024] The angle fixing device 9 includes a slide plate 902 and a rubber block 903. The outer wall of the slide plate 902 is slidably connected to the through hole opened on the surface of the support plate 3. The protruding part of the upper end of the slide plate 902 is fixedly connected to the rubber block 903. The rubber block 903 can prevent the slide plate 902 from quickly colliding with the support plate 3 during resetting and causing damage. The front end of the rubber block 903 fits with the support plate 3. The outer wall of the slide plate 902 and the support plate 3 are respectively fixedly connected to the two ends of the spring 906. The elastic coefficient of the spring 906 can meet the working needs according to actual needs. The spring 906 can drive the slide plate 902 to reset. The rear side of the slide plate 902 is fixedly connected to the handle 901. The slide plate 902 is movably connected to the rotating rod 905 through a pin shaft. The rear side of the support plate 3 is fixedly connected to the groove block 904. The groove opened on the surface of the groove block 904 is the same shape as the end of the rotating rod 905, so that one end of the rotating rod 905 can be inserted into the interior of the groove block 904.

[0025] The auxiliary pulling device 2 includes a reel 202 and a bracket 203. One end of the brackets 203 on both sides is fixedly connected to the support plate 3. The brackets 203 are rotatably connected to the rotating parts of the reel 202 through bearings to fix the rotation center of the reel 202. One side bracket 203 is fixedly connected to the second motor 201 through a motor frame. The second motor 201 can meet the work needs according to actual needs. The second motor 201 can drive the reel 202 to rotate. The output shaft of the second motor 201 is connected to the rotating part of the reel 202. The drum 202 is fixedly connected, and a pull rope 205 is wound around the outer wall of the drum 202. The drum 202 can reel in the pull rope 205 so that the pull rope 205 can pull the connecting block 206 through the fixed pulley 204 to move, so that the material box 5 can rotate toward a horizontal state. One end of the pull rope 205 is fixedly connected to the connecting block 206 through the fixed pulley 204. The fixed pulley 204 can change the pulling direction of the pull rope 205. The pulley frame of the fixed pulley 204 is fixedly connected to the upper end of the support plate 3, and the connecting block 206 is fixedly connected to the material box 5.

[0026] Working principle:

[0027] When extracting short nucleic acid molecules:

[0028] The sample reagent is added to the inside of the material box 5, and the extractant is added to the inside of the extraction tank 10 through the opening on the surface of the extraction tank 10. At the same time, the external power supply of the hydraulic cylinder 14 is turned on, and the telescopic end of the hydraulic cylinder 14 is started to extend to drive the second box body 11 to move synchronously, so that the second box body 11 can drive the extraction tank 10 to move synchronously, so that the extraction tank 10 enters the inside of the material box 5. The liquid level of the sample reagent in the material box 5 needs to be lower than the opening on the surface of the extraction tank 10. The external power supply of the first motor 15 is turned on, and the output shaft of the first motor 15 is started to rotate to drive the extraction tank 10 to rotate slowly, so that the liquid groove 8 provided on the surface of the extraction tank 10 can be more fully in contact with the sample reagent in the material box 5 (the function of the liquid groove 8 is the same as that in the authorization announcement number "CN220335165U"), so that the short nucleic acid molecules required in the sample reagent can penetrate into the extraction tank 10, and the extractant in the extraction tank 10 can extract the short nucleic acid molecules that penetrate into the extraction tank 10.

[0029] Analysis process:

[0030] After use, according to the above operation, the extraction tank 10 is restored to its original position, the bolts on the surface of the output shaft of the first motor 15 are rotated and removed, so that the extraction tank 10 can be disassembled, and the reagent inside the extraction tank 10 can be subjected to biological analysis.

[0031] Cleaning process:

[0032] The external power supply of the second motor 201 is turned on, and the output shaft of the second motor 201 is started to rotate and drive the reel 202 to move synchronously, so that the reel 202 can reel the pull rope 205, so that the pull rope 205 can pull the connecting block 206 to move through the fixed pulley 204, so that the connecting block 206 can drive the material box 5 to rotate slowly around the hinges with the second vertical plates 6 on both sides, so that the sample reagents in the material box 5 can be slowly poured out and collected. At the same time, the operator pulls the slide plate 902 through the handle 901 to move, so that the slide plate 902 presses the spring 90 6 is stretched, and the rotating rod 905 is pulled to a horizontal state, and the handle 901 is released. Under the action of the spring 906, one end of the rotating rod 905 can be inserted into the groove block 904, so that the position of the slide plate 902 is fixed, and the front end of the slide plate 902 can be in the same plane with the front side surface of the support plate 3, so that the material box 5 can be rotated to a state beyond the horizontal state. According to the above operation, the slide plate 902 is quickly returned to its original position, and the first motor 15 is stopped, so that the straight plate 4 is closely attached to the surface of the slide plate 902, so that the material box 5 can be in an inclined state, which is convenient for the operator to clean the inner wall of the material box 5.

[0033] Although the present invention has been shown and described with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein within the scope of the claims.

Claims

1. A short nucleic acid molecule extraction device for biological information analysis, comprising a bottom plate (1) and a first vertical plate (7), wherein the upper end of the bottom plate (1) is symmetrically fixedly connected to the first vertical plate (7), characterized in that: The upper ends of the first vertical plates (7) are fixedly connected to the top plate (12); the rear side of the upper end of the bottom plate (1) is fixedly connected to a support plate (3); the outer wall of the support plate (3) is provided with an angle fixing device (9); the upper end of the support plate (3) is provided with an auxiliary pulling device (2); the upper end of the bottom plate (1) is symmetrically fixedly connected to a second vertical plate (6); the second vertical plates (6) on both sides are movably connected to the protruding parts of the material box (5) through bearings.

2. A short nucleic acid molecule extraction device for biological information analysis according to claim 1, characterized in that: The angle fixing device (9) comprises a slide plate (902) and a rubber block (903); the outer wall of the slide plate (902) is slidably connected to a through hole provided on the surface of the support plate (3); the protruding portion at the upper end of the slide plate (902) is fixedly connected to the rubber block (903); the front end of the rubber block (903) is in contact with the support plate (3); the outer wall of the slide plate (902) and the support plate (3) are respectively fixedly connected to the two ends of a spring (906); the rear side of the slide plate (902) is fixedly connected to a handle (901); the slide plate (902) is movably connected to a rotating rod (905) via a pin shaft; and the rear side of the support plate (3) is fixedly connected to a groove block (904).

3. A short nucleic acid molecule extraction device for biological information analysis according to claim 1, characterized in that: The top plate (12) is fixedly connected to a first box body (13), the inner wall of the first box body (13) is fixedly connected to a hydraulic cylinder (14), the telescopic end of the hydraulic cylinder (14) passes through the first box body (13), and the lower end of the material box (5) is fixedly connected to a straight plate (4).

4. A short nucleic acid molecule extraction device for biological information analysis according to claim 3, characterized in that: The telescopic end of the hydraulic cylinder (14) is fixedly connected to a second box body (11), the inner wall of the second box body (11) is fixedly connected to the first motor (15) via a motor frame, and the surfaces of the first box body (13) and the second box body (11) are both provided with a plurality of through holes.

5. A short nucleic acid molecule extraction device for biological information analysis according to claim 4, characterized in that: The output shaft of the first motor (15) is rotatably connected to the second housing (11) via a bearing, the output shaft of the first motor (15) passes through the second housing (11), the output shaft of the first motor (15) is fixedly connected to the extraction tank (10) via bolts, and a plurality of liquid passage grooves (8) are provided on the surface of the extraction tank (10).

6. The short nucleic acid molecule extraction device for biological information analysis according to claim 1, characterized in that: The auxiliary pulling device (2) comprises a drum (202) and a bracket (203), one end of the bracket (203) on both sides is fixedly connected to the support plate (3), and the bracket (203) is rotatably connected to the rotating part of the drum (202) through bearings. The bracket (203) on one side is fixedly connected to the second motor (201) through a motor frame, and the output shaft of the second motor (201) is fixedly connected to the rotating part of the drum (202). A pull rope (205) is wound around the outer wall of the drum (202), and one end of the pull rope (205) is fixedly connected to a connecting block (206) through a fixed pulley (204), the pulley frame of the fixed pulley (204) is fixedly connected to the upper end of the support plate (3), and the connecting block (206) is fixedly connected to the material box (5).

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