Fluorescent PCR instrument

Abstract

The fluorescent PCR instrument comprises a shell, a control display screen and a detection mechanism, a centrifugal tube placing frame for placing a plurality of centrifugal tubes is fixedly connected in the shell, an upper heat insulation stone is fixedly arranged above the centrifugal tube placing frame, a lower heat insulation stone is arranged below the centrifugal tube placing frame, a main heating plate is arranged on the upper surface of the lower heat insulation stone, and the main heating plate is fixedly connected with the control display screen. An in-shell temperature controller is embedded in the side wall of the centrifugal tube placement frame, a heat preservation fixing shell is arranged in the shell, an incubation cover plate is rotationally connected to the upper portion of the shell, a heating auxiliary plate and a cover plate temperature controller are fixedly connected to the interior of the incubation cover plate, and flexible heating pieces are connected to the heating auxiliary plate and the main heating plate. According to the utility model, the temperature change of high temperature, low temperature and proper temperature can be realized, the temperature can be maintained, the accuracy of a fluorescence detection analysis result is ensured, and the detection precision of a fluorescence PCR instrument is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of PCR instruments, in particular to a fluorescent PCR instrument. Background Art

[0002] A PCR instrument is an instrument that uses PCR technology to amplify specific DNA. A fluorescent PCR instrument is an instrument that uses fluorescent chemicals to measure the total amount of products after each polymerase chain reaction cycle during the PCR amplification process, and performs quantitative analysis on specific DNA sequences in the sample to be tested through internal or external reference methods. It is widely used in life science research, biochemical analysis, clinical diagnosis, drug analysis, forensic identification, and rapid epidemic testing.

[0003] The existing patent number is CN218521253U, and the name is a Chinese patent for a real-time fluorescence quantitative PCR instrument, which includes a fluorescence quantitative PCR instrument, a control screen is provided inside the front end of the fluorescence quantitative PCR instrument, a protective plate is installed at the front end of the control screen, an indicator light is provided below the protective plate, a placement chamber is provided inside the front end of the fluorescence quantitative PCR instrument, a placement device seat is installed inside the placement chamber, a push seat is provided at the front end of the placement device seat, a push handle is provided at the front end of the push seat, the protective plate is rotatably connected to the fluorescence quantitative PCR instrument through a connecting hinge, and a handle is provided at the front end of the protective plate. The patent pushes the push handle to push the placement device seat to the original position, and then the protective plate can be opened by the handle. The installed protective plate can well protect the control screen to prevent the control screen from being easily damaged due to collision, and then the fluorescence quantitative PCR instrument is controlled by the control screen for detection.

[0004] The PCR reaction of fluorescent genes needs to complete a thermal cycle of high-temperature denaturation, low-temperature renaturation, and suitable temperature extension. Although the above-mentioned existing patents can protect the control screen to avoid damage caused by collision, it cannot achieve temperature changes of high temperature, low temperature and suitable temperature, nor can it maintain the temperature, which affects the fluorescence detection and analysis results, and further affects the detection accuracy of the fluorescence PCR instrument. Utility Model Content

[0005] The purpose of the present utility model is to overcome the shortcomings of the above-mentioned traditional technology and provide a fluorescent PCR instrument that can achieve temperature changes among high temperature, low temperature and suitable temperature, maintain each temperature, ensure the accuracy of the fluorescence detection and analysis results, and improve the detection accuracy of the fluorescent PCR instrument.

[0006] The purpose of this utility model is achieved through the following technical measures:

[0007] A fluorescent PCR instrument comprises a shell, a control display screen and a detection mechanism, wherein the detection mechanism is arranged in the shell, the control display screen is arranged on the side wall of the shell, a centrifuge tube placement rack for placing multiple centrifuge tubes is fixedly connected in the shell, an upper insulation stone is fixedly arranged above the centrifuge tube placement rack, a lower insulation stone is arranged below the centrifuge tube placement rack, a main heating plate is arranged on the upper surface of the lower insulation stone, an in-shell temperature controller is embedded on the side wall of the centrifuge tube placement rack, a heat-insulating fixed shell surrounding the upper insulation stone, the lower insulation stone and the centrifuge tube placement rack is arranged in the shell, an incubation cover plate is rotatably connected above the shell, a heating auxiliary plate and a cover plate temperature controller are fixedly connected in the incubation cover plate, a flexible heating sheet is connected to the heating auxiliary plate and the main heating plate, and the detection mechanism, the heating auxiliary plate, the cover plate temperature controller, the main heating plate, the in-shell temperature controller and the flexible heating sheet are all electrically connected to the control display screen.

[0008] Further specific optimization is that a light-proof convex frame is fixedly arranged above the centrifuge tube placement rack, and a light-proof pressure groove compatible with the heat-insulating convex frame is opened on the incubation cover plate, and the light-proof convex frame is arranged above the upper insulation stone.

[0009] According to further specific optimization, the centrifuge tube placement rack is provided with a plurality of centrifuge tube placement slots, and a detection through hole is opened on the side wall of each of the centrifuge tube placement slots, and the detection through hole runs through the centrifuge tube placement rack.

[0010] Further specific optimization, the detection mechanism includes a detection motor, a transmission belt and a fluorescent detection head, the detection motor is fixed in a shell, a main transmission wheel is sleeved on the output shaft of the detection motor, a slave transmission wheel parallel to the main transmission wheel is rotatably arranged in the shell, the main transmission wheel and the slave transmission wheel are rotatably connected to the inner side of the transmission belt, the fluorescent detection head is connected to the transmission belt, and the fluorescence of the fluorescent detection head can pass through the detection through hole.

[0011] According to further specific optimization, a micro switch is fixedly arranged in the shell, a button head is connected to the micro switch, the button head can conflict with the incubation cover plate, and the micro switch is electrically connected to the control display screen.

[0012] Further specific optimization is that two limit columns are symmetrically arranged on the heat-insulating fixed shell, and a limit spring is vertically arranged in each of the limit columns. One end of each limit spring away from the limit column is fixedly connected to a limit round head, and a rotating connecting shaft is arranged on the incubation cover plate. Both ends of the rotating connecting shaft are fixedly connected with limit blocks that cooperate with the limit round head for limiting, and the limit block is provided with a limit groove used in conjunction with the limit circle.

[0013] According to further specific optimization, two magnet blocks with corresponding positions are fixedly arranged on the heat-insulating fixed shell and the incubation cover plate.

[0014] Due to the adoption of the above technical solution, compared with the prior art, the advantages of the utility model are:

[0015] The utility model discloses a fluorescent PCR instrument, which heats up and down a main heating plate and a heating auxiliary plate by turning on electricity, and stops heating by turning off electricity, and then performs heat insulation and heat preservation through an upper heat insulation stone, a lower heat insulation stone and a heat-insulating fixed shell, so as to realize the thermal cycle of high-temperature denaturation, low-temperature renaturation and suitable-temperature extension required for the PCR reaction of the fluorescent gene, realize the temperature change of high temperature, low temperature and suitable temperature, maintain each temperature, ensure the accuracy of the fluorescence detection analysis result, and improve the detection accuracy of the fluorescent PCR instrument.

[0016] The utility model is further described below in conjunction with the accompanying drawings and specific implementation methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the utility model.

[0018] Figure 2 It is a structural schematic diagram of a centrifuge tube placement rack according to an embodiment of the utility model.

[0019] Figure 3 It is a schematic diagram of the internal structure of the incubation cover plate of an embodiment of the utility model.

[0020] Figure 4 It is a structural schematic diagram of the detection mechanism of an embodiment of the utility model.

[0021] Figure 5 It is a structural schematic diagram of the main heating plate of an embodiment of the utility model.

[0022] Figure 6 It is a structural schematic diagram of a heat-insulating fixed shell according to an embodiment of the utility model.

[0023] Figure 7 It is a schematic structural diagram of a limit block and a limit column in an embodiment of the utility model.

[0024] Figure 8 It is a structural schematic diagram of a micro switch and a button head according to an embodiment of the utility model.

[0025] Fig. 9 It is a schematic diagram of the cross-sectional structure of the limiting column of an embodiment of the utility model.

[0026] Markings in the figure: 1. Shell; 2. Control display screen; 3. Centrifuge tube placement rack; 4. Main heating plate; 5. Shell temperature controller; 6. Incubation cover; 7. Heating auxiliary plate; 8. Cover temperature controller; 9. Light-proof convex frame; 10. Light-proof pressure groove; 11. Flexible heating plate; 12. Upper insulation stone; 13. Lower insulation stone; 14. Insulation fixed shell; 15. Detection through hole; 16. Detection motor; 17. Transmission belt; 18. Fluorescence detection head; 19. Main transmission wheel; 20. Slave transmission wheel; 21. Micro switch; 22. Button head; 23. Limit column; 24. Limit spring; 25. Limit round head; 26. Limit card block; 27. Magnet block; 28. Limit groove. DETAILED DESCRIPTION

[0027] In order to make the purpose and technical solution of the utility model clearly and completely described, and the advantages more clearly understood, the embodiments of the utility model are further described in detail in conjunction with the accompanying drawings. It should be understood that the specific embodiments described here are part of the embodiments of the utility model, not all of the embodiments, and are only used to explain the embodiments of the utility model, and are not used to limit the embodiments of the utility model. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0028] In the description of the present utility model, it should be noted that the terms "center", "middle", "upper", "lower", "left", "right", "inner", "outer", "top", "bottom", "side", "vertical", "horizontal" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "one", "first", "second", "third", "fourth", "fifth", "sixth" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0029] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] For the purpose of simplicity and illustration, the principles of the embodiments are mainly described by reference to examples. In the following description, many specific details are proposed to provide a thorough understanding of the embodiments. However, it is obvious that for those of ordinary skill in the art, these embodiments may not be limited to these specific details in practice. In some instances, known methods and structures are not described in detail to avoid making these embodiments unnecessarily difficult to understand. In addition, all embodiments can be used in combination with each other.

[0031] Example:

[0032] A fluorescent PCR instrument comprises a shell 1, a control display screen 2 and a detection mechanism, wherein the detection mechanism is arranged in the shell 1, the control display screen is arranged on the side wall of the shell 1, a centrifuge tube placement rack 3 for placing multiple centrifuge tubes is fixedly connected in the shell 1, an upper insulation stone 12 is fixedly arranged above the centrifuge tube placement rack 3, a lower insulation stone 13 is arranged below the centrifuge tube placement rack 3, a main heating plate 4 is arranged on the upper surface of the lower insulation stone 13, an in-shell temperature controller 5 is embedded on the side wall of the centrifuge tube placement rack 3, a heat-insulating fixed shell 14 surrounding the upper insulation stone 12, the lower insulation stone 13 and the centrifuge tube placement rack 3 is arranged in the shell 1, an incubation cover plate 6 is rotatably connected above the shell 1, a heating auxiliary plate 7 and a cover plate temperature controller 8 are fixedly connected in the incubation cover plate 6, a flexible heating plate 11 is connected to the heating auxiliary plate 7 and the main heating plate 4, and the detection mechanism, the heating auxiliary plate 7, the cover plate temperature controller 8, the main heating plate 4, the in-shell temperature controller 5 and the flexible heating plate 11 are all electrically connected to the control display screen 2.

[0033] Through the above technical scheme, the incubation cover 6 is opened to place multiple centrifuge tubes that need reaction detection on the centrifuge tube placement rack 3, and then the incubation cover 6 is closed. According to the reaction needs, the main heating plate 4, the heating auxiliary plate 7 and the flexible heating plate 11 are controlled by the control display to control the heating or disconnection, thereby controlling the reaction temperature of the centrifuge tube. The upper insulation stone 12, the lower insulation stone 13 and the heat-insulating fixed shell 14 are used to realize the thermal cycle of high-temperature denaturation, low-temperature renaturation and suitable temperature extension, and each temperature is maintained. When the reaction is completed and needs to be detected, the detection mechanism performs fluorescence detection on the centrifuge tube, thereby ensuring the accuracy of the fluorescence detection analysis results and improving the detection accuracy of the fluorescence PCR instrument.

[0034] A light-proof convex frame 9 is fixedly arranged above the centrifuge tube placement rack 3, and a light-proof pressure groove 10 adapted to the heat-insulating convex frame is provided on the incubation cover 6, and the light-proof convex frame 9 is arranged above the upper heat-insulating stone 12. When the incubation cover 6 is closed, the light-proof convex frame 9 is embedded in the corresponding light-proof pressure groove 10, thereby ensuring that the light irradiated from the transparent window on the incubation cover 6 above the centrifuge tube placement rack 3 cannot pass through, and other light sources are prevented from entering the housing 1, thereby improving the accuracy of the fluorescence detection results.

[0035] The centrifuge tube placement rack 3 is provided with a plurality of centrifuge tube placement slots, and a detection through hole 15 is provided on the side wall of each centrifuge tube placement slot. The detection through hole 15 is provided through the centrifuge tube placement rack 3 .

[0036] The detection mechanism includes a detection motor 16, a transmission belt 17 and a fluorescent detection head 18. The detection motor 16 is fixed in the shell 1. A main transmission wheel 19 is sleeved on the output shaft of the detection motor 16. A slave transmission wheel 20 parallel to the main transmission wheel 19 is rotatably arranged in the shell 1. The main transmission wheel 19 and the slave transmission wheel 20 are rotatably connected to the inner side of the transmission belt 17. The fluorescent detection head 18 is connected to the transmission belt 17. The fluorescence of the fluorescent detection head 18 can pass through the detection through hole 15.

[0037] Through the above technical solution, the detection motor 16 rotates to drive the main transmission wheel 19 and the slave transmission wheel 20 to rotate, and then drives the transmission belt 17 to rotate, and controls the fluorescent detection head 18 to emit detection light along each detection through hole 15 in sequence, irradiates the detection light through the detection through hole 15 to the separation tube, and then reflects the light through the separation tube to obtain the detection result of the separation tube, and then feeds back the fluorescent detection result to the control display screen 2 for display.

[0038] A micro switch 21 is fixedly arranged in the shell 1, and a button head 22 is connected to the micro switch 21. The button head 22 can interfere with the incubation cover 6. The micro switch 21 is electrically connected to the control display screen 2. When the incubation cover 6 is rotated and closed, the button head 22 is squeezed. When it is fully closed, the micro switch 21 is triggered, and the control display screen 2 displays that the incubation cover 6 is closed, which can ensure the dissipation of heat in the shell 1.

[0039] Two limit columns 23 are symmetrically arranged on the heat-insulating fixed shell 14, and a limit spring 24 is vertically arranged in each limit column 23. One end of each limit spring 24 away from the limit column 23 is fixedly connected to a limit round head 25. A rotating connecting shaft is arranged on the incubation cover 6, and both ends of the rotating connecting shaft are fixedly connected with limit blocks 26 that cooperate with the limit round head 25 for limiting the position. The limit block 26 is provided with a limit groove 28 used in conjunction with the limit circle. Tilting the incubation cover 6 drives the rotating connecting shaft to rotate, and then drives the limit block 26 to rotate at both ends of the rotating connecting shaft. The limit block 26 conflicts with the limit round head 25 when rotating, the limit spring 24 is squeezed, and the limit round head 25 retracts into the limit column 23. When the limit block 26 rotates to the position where the limit groove 28 is relative to the limit round tube, the limit round head 25 slides out of the limit column 23 and then enters the limit groove 28, thereby positioning and limiting the incubation cover 6, freeing the operator's hands and improving detection efficiency.

[0040] Two magnet blocks 27 corresponding to each other are fixedly arranged on the heat preservation fixed shell 14 and the incubation cover 6. When the incubation cover 6 is closed, the four magnets are adsorbed in pairs, which can ensure the stability of the incubation cover 6 when it is closed and avoid temperature loss.

[0041] When the fluorescent PCR instrument is in use, the incubation cover 6 is opened and multiple centrifuge tubes that need to be reacted and tested are placed on the centrifuge tube placement rack 3, and then the incubation cover 6 is closed. When the incubation cover 6 is closed, the button head 22 is squeezed to trigger the micro switch 21 to ensure that the incubation cover 6 is closed before the next temperature control operation can be carried out. Subsequently, according to the reaction needs, the main heating plate 4, the heating auxiliary plate 7 and the flexible heating plate 11 are controlled by the control display to heat or disconnect, thereby controlling the reaction temperature of the centrifuge tube. The upper insulation stone 12, the lower insulation stone 13 and the heat-insulating fixed shell 14 are used to realize the thermal cycle of high-temperature denaturation, low-temperature renaturation and suitable temperature extension, and each temperature is maintained. When the reaction is completed and needs to be detected, the fluorescent detection head 18 emits detection light along each detection through hole 15 in sequence, irradiates the separation tube through the detection through hole 15, and then obtains the detection result of the separation tube through the reflected light of the separation tube, thereby ensuring the accuracy of the fluorescence detection analysis result and improving the detection accuracy of the fluorescent PCR instrument.

[0042] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0043] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A fluorescence PCR instrument, comprising a housing (1), a control display screen (2) and a detection mechanism, wherein the detection mechanism is arranged in the housing (1), and the control display screen is arranged on a side wall of the housing (1), characterized in that: A centrifuge tube placement rack (3) for placing a plurality of centrifuge tubes is fixedly connected to the shell (1), an upper heat-insulating stone (12) is fixedly arranged above the centrifuge tube placement rack (3), a lower heat-insulating stone (13) is arranged below the centrifuge tube placement rack (3), a main heating plate (4) is arranged on the upper surface of the lower heat-insulating stone (13), an in-shell temperature controller (5) is embedded on the side wall of the centrifuge tube placement rack (3), and a heat-insulating device (5) is arranged in the shell (1) for placing the upper heat-insulating stone (12), the lower heat-insulating stone (13) and the centrifuge tube placement rack (3). A heat-insulating fixed shell (14) is surrounded by a rack (3); an incubation cover plate (6) is rotatably connected to the top of the shell (1); a heating auxiliary plate (7) and a cover plate thermostat (8) are fixedly connected inside the incubation cover plate (6); a flexible heating sheet (11) is connected to the heating auxiliary plate (7) and the main heating plate (4); the detection mechanism, the heating auxiliary plate (7), the cover plate thermostat (8), the main heating plate (4), the shell thermostat (5) and the flexible heating sheet (11) are all electrically connected to the control display screen (2).

2. The fluorescent PCR instrument according to claim 1, characterized in that: A light-proof convex frame (9) is fixedly arranged above the centrifuge tube placement rack (3), and a light-proof pressure groove (10) adapted to the heat-insulating convex frame is provided on the incubation cover plate (6), and the light-proof convex frame (9) is arranged above the upper heat-insulating stone (12).

3. The fluorescent PCR instrument according to claim 1, characterized in that: The centrifuge tube placement rack (3) is provided with a plurality of centrifuge tube placement slots, and a detection through hole (15) is provided on the side wall of each centrifuge tube placement slot. The detection through hole (15) is arranged through the centrifuge tube placement rack (3).

4. The fluorescent PCR instrument according to claim 3, characterized in that: The detection mechanism comprises a detection motor (16), a transmission belt (17) and a fluorescent detection head (18); the detection motor (16) is fixed in a housing (1); a main transmission wheel (19) is sleeved on the output shaft of the detection motor (16); a slave transmission wheel (20) parallel to the main transmission wheel (19) is rotatably arranged in the housing (1); the main transmission wheel (19) and the slave transmission wheel (20) are rotatably connected to the inner side of the transmission belt (17); the fluorescent detection head (18) is connected to the transmission belt (17); and the fluorescence of the fluorescent detection head (18) can pass through the detection through hole (15).

5. The fluorescent PCR instrument according to any one of claims 1 to 4, characterized in that: A micro switch (21) is fixedly arranged in the housing (1), and a button head (22) is connected to the micro switch (21). The button head (22) can be in contact with the incubation cover plate (6), and the micro switch (21) is electrically connected to the control display screen (2).

6. The fluorescent PCR instrument according to claim 1, characterized in that: Two limit posts (23) are symmetrically arranged on the heat-insulating fixed shell (14), and a limit spring (24) is vertically arranged in each of the limit posts (23). One end of each limit spring (24) away from the limit post (23) is fixedly connected to a limit round head (25). A rotating connecting shaft is arranged on the incubation cover (6), and both ends of the rotating connecting shaft are fixedly connected to limit blocks (26) that cooperate with the limit round head (25) for limiting, and a limit groove (28) used in conjunction with the limit circle is opened on the limit block (26).

7. The fluorescent PCR instrument according to claim 1, characterized in that: The heat-insulating fixed shell (14) and the incubation cover plate (6) are both fixedly provided with two magnet blocks (27) at corresponding positions.

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