3D (three-dimensional) organoid culture plate
By using technical means such as transverse tubes and leaking holes on the organoid culture plate, the rapid addition of culture medium is achieved, and the time-consuming addition of culture medium in the existing technology is solved, which improves work efficiency and is easy to use.
Patent Information
- Application Number
- CN202421896291.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-06
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2034-08-06
AI Technical Summary
During the use of existing organoid culture plates, the culture medium needs to be manually added every once in a while, which takes a long time and leads to inefficient work.
A 3D organoid culture plate was designed, using technical means such as horizontal tube, leakage hole, L-shaped limiting plate, through hole, right-angle triangle block, first spring, bump, press block, insert rod, barrier ring and second spring. By rotating the through tube and horizontal tube, the culture liquid can be quickly and evenly added to the culture hole.
The rapid addition of culture medium is achieved, the user's work efficiency is improved, and the equipment is easy to use. The rubber sleeve and anti-slip texture are set to prevent the culture plate from sliding, increasing the convenience of use.
Smart Images
Figure CN222948376U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of organoid culture, in particular to a 3D organoid culture plate. Background Art
[0002] Organoids are a type of three-dimensional cell culture that is formed by differentiation of stem cells or progenitor cells in an in vitro culture environment and has certain organ structure and functional characteristics. Organoids are generally cultured on culture plates, which can provide cells with a suitable growth environment and improve the success rate and quality of organoid culture.
[0003] In the prior art, when the existing organoid culture plate is in use, culture fluid needs to be added to the culture wells at regular intervals. Since there are a large number of culture wells, it takes a long time to add culture fluid on each side, resulting in reduced work efficiency and inconvenience for users. For this reason, a 3D organoid culture plate is designed. Utility Model Content
[0004] The utility model aims to solve the shortcomings in the prior art and proposes a 3D organoid culture plate.
[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:
[0006] A 3D organoid culture plate comprises a culture plate body and a cover plate, wherein a plurality of culture holes are equidistantly arranged on the top circumference of the culture plate body, a buckle groove is arranged on the circumferential edge of the top circumferential edge of the culture plate body, and the buckle groove is matched with the cover plate, both ends of the buckle groove are fixedly connected to limit bars, a slot matched with the limit bar is arranged on the inner wall of the circumference of the bottom circumference of the cover plate, a through pipe is rotatably sleeved on the middle position of the top of the cover plate, a circular hole matched with the through pipe is arranged in the middle of the cover plate, a transverse tube is fixedly connected to the bottom of the through pipe, three leakage holes are equidistantly arranged on the bottom of the transverse tube, and the leakage holes are aligned with the limit bar. The corresponding culture holes are adapted, and L-shaped limit plates are fixedly connected on both sides of the bottom of the horizontal tube. The same sliding bar is fixedly connected between the two L-shaped limit plates. The sliding bar is provided with three through holes at equal distances, and the through holes are adapted to the sliding bar. The two L-shaped limit plates are fixedly connected to the same baffle plate at one end close to the through tube, and the sliding bar is fixedly connected to a fixed block at one end close to the baffle. The fixed block is fixedly connected to a sliding rod at one end close to the baffle, and the other end of the sliding rod passes through the baffle and is fixedly connected to a protrusion, and the first spring is sleeved on one end of the sliding rod close to the fixed block.
[0007] As a further solution of the utility model, an upper hopper is fixedly connected to the top of the through pipe, and a rubber cover is provided on the upper hopper.
[0008] As a further solution of the utility model, a rotating block is fixedly sleeved on the circumferential surface of the through pipe.
[0009] As a further solution of the utility model, a plurality of trigger mechanisms are arranged at equal distances at the top edge of the cover plate, and the trigger mechanism includes an insertion rod slidably sleeved on the edge of the cover plate, the insertion rod is located at the bottom of the cover plate and a retaining ring is fixedly sleeved on the circumferential surface, a pressing block is fixedly connected to the top of the insertion rod, the insertion rod is located at the top of the cover plate and a sliding ring is slidably sleeved, and a second spring is sleeved on the insertion rod between the pressing block and the sliding ring.
[0010] As a further solution of the utility model, one end of the sliding bar away from the through pipe is fixedly connected to a right-angled triangle block, and the right-angled triangle block is adapted to the insertion rod.
[0011] As a further solution of the utility model, a rubber sleeve is provided at the bottom of the culture plate body, and the outer circumferential surface of the rubber sleeve has anti-slip patterns.
[0012] The beneficial effects of the utility model are:
[0013] The utility model: due to the adoption of technical means such as a transverse tube, a leakage hole, an L-shaped limit plate, a through hole, a right-angle triangle block, a first spring, a protrusion, a push block, an insertion rod, a retaining ring and a second spring, the through tube rotates with the transverse tube by rotating the rotating block, so that the transverse tube is located at the top of a group of culture holes to which culture fluid needs to be added, and the culture fluid of each group of culture holes can be quickly added by pressing the push block, which effectively solves the problems raised in the background technology, thereby realizing the technical effect of quickly and conveniently adding culture fluid to the culture holes, improving the work efficiency of users, and making the device easy to use.
[0014] The utility model provides a rubber sleeve and anti-skid patterns, so that the culture plate body is not easy to slide when placed on a desktop or a workbench, and the anti-skid patterns play an anti-skid role when the user holds it up. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 This is a schematic diagram of the overall disassembly structure of a 3D organoid culture plate proposed in the utility model;
[0016] Figure 2 This is a schematic cross-sectional structure diagram of a cover plate of a 3D organoid culture plate proposed in the utility model;
[0017] Figure 3 This is a schematic diagram of the structure of the bottom of the horizontal tube of a 3D organoid culture plate proposed by the utility model;
[0018] Figure 4 A 3D organoid culture plate proposed in the utility model Figure 3 A schematic diagram of the structure enlarged at A;
[0019] Figure 5 This is a schematic structural diagram of the insertion rod of a 3D organoid culture plate proposed in the utility model.
[0020] In the figure: 1. rubber sleeve; 2. anti-slip pattern; 3. culture plate body; 4. culture hole; 5. buckle groove; 501. limit strip; 6. cover plate; 7. rotating block; 8. through pipe; 9. upper hopper; 10. rubber cover; 11. horizontal pipe; 12. leakage hole; 13. L-shaped limit plate; 14. baffle; 15. sliding strip; 16. through hole; 17. right-angle triangle block; 18. fixed block; 19. sliding rod; 20. first spring; 21. protrusion; 22. push block; 23. plug rod; 24. sliding ring; 25. baffle ring; 26. second spring. DETAILED DESCRIPTION
[0021] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.
[0022] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present utility model will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.
[0023] Reference Figure 1-Figure 5 A 3D organoid culture plate comprises a culture plate body 3 and a cover plate 6. A plurality of culture wells 4 are equidistantly arranged on the top circumference of the culture plate body 3. A buckle groove 5 is arranged on the circumferential edge of the top of the culture plate body 3, and the buckle groove 5 is matched with the cover plate 6. Both ends of the buckle groove 5 are fixedly connected with a limiting strip 501. The setting of the limiting strip 501 ensures that when the cover plate 6 is covered, each insertion rod 23 is located at each corresponding group of culture wells 4. A slot matched with the limiting strip 501 is arranged on the inner wall of the circumference of the bottom of the cover plate 6. A through pipe 8 is rotatably sleeved on the middle position of the top of the cover plate 6. A circular hole matched with the through pipe 8 is arranged in the middle of the cover plate 6. A transverse tube 11 is fixedly connected to the bottom of the through pipe 8. Three equidistant holes are arranged on the bottom of the transverse tube 11. Leakage holes 12 are provided, and the leakage holes 12 are matched with the corresponding culture holes 4. L-shaped limiting plates 13 are fixedly connected to both sides of the bottom of the transverse tube 11. A sliding bar 15 is fixedly connected between the two L-shaped limiting plates 13. Three through holes 16 are provided on the sliding bar 15 at equal distances, and the through holes 16 are matched with the sliding bar 15. The two L-shaped limiting plates 13 are fixedly connected to a baffle 14 at one end close to the through tube 8. A fixed block 18 is fixedly connected to the end of the sliding bar 15 close to the baffle 14. A sliding rod 19 is fixedly connected to the end of the fixed block 18 close to the baffle 14, and the other end of the sliding rod 19 passes through the baffle 14 and is fixedly connected to a protrusion 21. A first spring 20 is sleeved on the end of the sliding rod 19 close to the fixed block 18.
[0024] In this embodiment, an upper hopper 9 is fixedly connected to the top of the through pipe 8, and a rubber cover 10 is provided on the upper hopper 9. The rubber cover 10 can seal the upper hopper 9 when no culture solution is added.
[0025] In this embodiment, a rotating block 7 is fixedly sleeved on the circumferential surface of the through pipe 8 , and the rotating block 7 is convenient for the user to rotate to adjust the direction of the transverse pipe 11 .
[0026] In this embodiment, multiple trigger mechanisms are equidistantly arranged at the top edge of the cover plate 6, and the trigger mechanism includes an insertion rod 23 slidably sleeved on the edge of the cover plate 6, the insertion rod 23 is located at the bottom of the cover plate 6 and a retaining ring 25 is fixedly sleeved on the circumferential surface, a pressing block 22 is fixedly connected to the top of the insertion rod 23, and a sliding ring 24 is slidably sleeved at the top of the cover plate 6. A second spring 26 is sleeved on the insertion rod 23 between the pressing block 22 and the sliding ring 24.
[0027] In this embodiment, the end of the sliding bar 15 away from the through tube 8 is fixedly connected to a right-angled triangle block 17, and the right-angled triangle block 17 is adapted to the insertion rod 23. By pressing the pressing block 22, the insertion rod 23 pushes the right-angled triangle block 17, thereby realizing the movement of the sliding bar 15 and the dripping of the culture solution.
[0028] In this embodiment, a rubber sleeve 1 is provided at the bottom of the culture plate body 3, and an anti-slip pattern 2 is provided on the circumferential outer surface of the rubber sleeve 1. The arrangement of the rubber sleeve 1 and the anti-slip pattern 2 makes the culture plate body 3 not easy to slide on a desktop or workbench.
[0029] Working principle: When using, when organ cell culture is carried out through multiple culture wells 4, the cells to be cultured and the nutrient solution are added to the culture wells 4, and then the cover 6 is closed. The cover 6 is a transparent acrylic structure, and observation can be made through the cover 6. When the culture solution needs to be added later, the amount of the culture solution is calculated according to the number of each group of culture wells 4, and the rubber cover 10 is opened. An appropriate amount of culture solution is passed through the cross tube 11 of the upper hopper 9. The cross tube 11 is rotated by the rotating block 7 so that the cross tube 11 is located above the group of culture wells 4 to which the culture solution needs to be added. At this time, the right-angled triangle block 17 will be located at the bottom of the corresponding insertion rod 23. At this time, the pressing block 22 is pressed to make the insertion rod 23 The rod 23 descends, compressing the second spring 26. The bottom of the rod 23 contacts the inclined surface of the right-angled triangle block 17, so that the right-angled triangle block 17 moves with the sliding bar 15 and compresses the first spring 20. At this time, each through hole 16 of the sliding bar 15 is aligned with the leakage hole 12. At this time, the culture fluid will drip into the corresponding culture wells 4 through the sliding bar 15 and the through holes 16 at the same time. The rotating block 7 is continuously rotated to quickly and evenly add the culture fluid to each group of culture wells 4, thereby realizing the rapid addition of the culture fluid. The setting of the rubber sleeve 1 and the anti-slip pattern 2 makes it difficult for the culture plate body 3 to slide when placed on a desktop or workbench, and the anti-slip pattern 2 plays an anti-slip role when the user holds it up.
[0030] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.
[0031] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.
[0032] The above description is only the preferred embodiment of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.
Claims
1. A 3D organoid culture plate, comprising a culture plate body (3) and a cover plate (6), characterized in that: The top circumference of the culture plate body (3) is provided with a plurality of culture holes (4) at equal distances. The top circumferential edge of the culture plate body (3) is provided with a buckle groove (5), and the buckle groove (5) is matched with the cover plate (6). Both ends of the buckle groove (5) are fixedly connected with a limit strip (501). The inner wall of the bottom circumference of the cover plate (6) is provided with a slot matched with the limit strip (501). A through pipe (8) is rotatably sleeved at the middle position of the top of the cover plate (6). A circular hole matched with the through pipe (8) is provided in the middle of the cover plate (6). The bottom of the through pipe (8) is fixedly connected with a transverse pipe (11). The bottom of the transverse pipe (11) is provided with three leakage holes (12) at equal distances, and the leakage holes (12) are matched with the corresponding culture holes (4). The bottom of the transverse pipe (11) has two sides. Both are fixedly connected with an L-shaped limit plate (13), a same sliding bar (15) is fixedly connected between the two L-shaped limit plates (13), three through holes (16) are opened at equal distances on the sliding bar (15), and the through holes (16) are adapted to the sliding bar (15), the two L-shaped limit plates (13) are fixedly connected with a same baffle (14) at one end close to the through pipe (8), a fixed block (18) is fixedly connected at one end of the sliding bar (15) close to the baffle (14), a sliding rod (19) is fixedly connected at one end of the fixed block (18) close to the baffle (14), and the other end of the sliding rod (19) penetrates the baffle (14) and is fixedly connected with a protrusion (21), and a first spring (20) is sleeved on one end of the sliding rod (19) close to the fixed block (18).
2. The 3D organoid culture plate according to claim 1, characterized in that The top of the through pipe (8) is fixedly connected to an upper hopper (9), and a rubber cover (10) is provided on the upper hopper (9).
3. The 3D organoid culture plate according to claim 1, characterized in that: A rotating block (7) is fixedly sleeved on the circumferential surface of the through pipe (8).
4. The 3D organoid culture plate according to claim 1, characterized in that: A plurality of trigger mechanisms are arranged at equal distances at the top edge of the cover plate (6), the trigger mechanism comprising an insertion rod (23) slidably sleeved on the edge of the cover plate (6), a retaining ring (25) is fixedly sleeved on the circumferential surface of the insertion rod (23) located at the bottom of the cover plate (6), a pressing block (22) is fixedly connected to the top of the insertion rod (23), a sliding ring (24) is slidably sleeved on the insertion rod (23) located at the top of the cover plate (6), and a second spring (26) is sleeved on the insertion rod (23) located between the pressing block (22) and the sliding ring (24).
5. The 3D organoid culture plate according to claim 1, characterized in that: One end of the sliding bar (15) away from the through pipe (8) is fixedly connected to a right-angled triangle block (17), and the right-angled triangle block (17) is adapted to the insertion rod (23).
6. The 3D organoid culture plate according to claim 1, characterized in that: The bottom of the culture plate body (3) is covered with a rubber sleeve (1), and the outer circumferential surface of the rubber sleeve (1) is provided with anti-slip grooves (2).