Multi-mode microscopic imaging system
By designing a multi-mode micro imaging system, including white light field, fluorescence and panoramic imaging modules, the problem of sample position and angle changes when switching between different modes is solved, seamless switching and high-precision control are achieved, and observation effect and work efficiency are improved.
Patent Information
- Application Number
- CN202421659981.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-12
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2034-07-12
AI Technical Summary
When existing microscopes switch between different imaging modes, they need to reposition the sample, which causes the sample position and angle to change, affect the observation effect, and are cumbersome to operate and reduce work efficiency.
A multi-mode micro-imaging system is designed, including a white light field imaging module, a fluorescence imaging module and a panoramic imaging module. Through the design of position adjustment components and panoramic imaging positions, seamless switching between different imaging modes is achieved to avoid changes in sample position and angle.
Simple switching between different imaging modes is realized, without moving the sample body, avoiding the problem of non-coining image positions, improving observation effect and working efficiency, and high-precision control is achieved through computers or electrical modules.
Smart Images
Figure CN222952196U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microscopic imaging, in particular to a multi-mode microscopic imaging system. Background Art
[0002] Currently, microscopes are divided into two categories, namely fluorescence microscopes and ordinary optical microscopes. Ordinary optical microscopes observe samples with ordinary light sources, and can image samples in color and black and white. Unlike ordinary optical microscopes, fluorescence microscopes use light of a certain wavelength (ultraviolet light, blue-violet light) to excite the fluorescent substances in the specimens under the microscope to emit fluorescence. The role of the light source of a fluorescence microscope is not direct illumination, but as an energy source to excite the fluorescent substances in the specimens. The specimens are observed through the fluorescence phenomenon presented by the fluorescent substances in the specimens after absorbing the excitation light energy. The light source of a fluorescence microscope can supply a large amount of excitation light in a specific wavelength range, so that the fluorescent substances in the specimens under examination can obtain the necessary intensity of excitation light.
[0003] After observing and measuring the fluorescence pattern of the sample through a fluorescence microscope, the user often needs to observe the white light image and panoramic image of the sample, which requires switching to an ordinary optical microscope for operation. Replacing the sample will cause the position and angle of the sample to change, resulting in the image position observed under the ordinary optical microscope and the image position under the fluorescence microscope not overlapping, seriously affecting the observation effect; and switching between different microscopes is cumbersome and reduces work efficiency.
[0004] Therefore, a microscopic imaging system with multiple imaging modes is very necessary. Utility Model Content
[0005] The purpose of the utility model is to overcome the above-mentioned technical deficiencies and provide a multi-mode microscopic imaging system, which is simple to operate and does not require moving the sample body while ensuring the diversity of sample detection, thus avoiding the problem that the image position observed by the optical microscope in different modes and the image position of the fluorescence microscope do not overlap, which seriously affects the observation effect.
[0006] In order to achieve the above technical objectives, the technical solution of the utility model provides a single-channel sample sorting device, which includes:
[0007] A white light bright field imaging module, the white light bright field imaging module comprising a microscope imaging component and a bright field imaging position, the white light bright field imaging module being configured to perform bright field imaging of a sample to be tested disposed at the bright field imaging position through the microscope imaging component;
[0008] A fluorescence imaging module, the fluorescence imaging module comprising at least one fluorescence light source component and a position adjustment component, the fluorescence light source component being configured to be inserted into or removed from the imaging light path of the microscope imaging component through the position adjustment component;
[0009] A panoramic imaging module, the panoramic imaging module includes a panoramic camera and a panoramic imaging position, the panoramic camera is configured to perform panoramic imaging of a sample to be tested set at the panoramic imaging position in a panoramic imaging mode.
[0010] Preferably, the microscope imaging assembly includes a microscopic camera, an objective lens, a stage and a bright field light source, the microscopic camera and the objective lens are arranged on the same axis, and the focus of the microscopic camera and the focus of the objective lens are also located on the same axis.
[0011] Preferably, a storage slot with a transmission window is provided on the storage table, the objective lens and the bright field light source are arranged at the upper and lower ends of the transmission window relative to each other, and the microscopic camera, objective lens, transmission window and bright field light source are all located on the same axis.
[0012] Preferably, the position adjustment component includes a displacement track that vertically passes through the imaging light path of the microscope imaging component, and the fluorescent light source component is movably mounted on the displacement track through a slider, and the displacement track is configured to drive the fluorescent light source component to move into the imaging light path of the microscope imaging component or move out of the imaging light path of the microscope imaging component.
[0013] Preferably, a plurality of fluorescent light source components are movablely mounted on the displacement track. In the panoramic imaging mode, the displacement track is configured to drive the plurality of fluorescent light source components to move so that one of the plurality of fluorescent light source components is inserted into the imaging light path of the microscope imaging assembly.
[0014] Preferably, a transmission channel is provided in the fluorescent light source component, and the transmission channel is configured so that when the fluorescent light source component is inserted into the imaging light path of the microscope imaging assembly, the transmission channel is coaxially arranged with the imaging light path; a semi-transparent and semi-reflective film is provided in the transmission channel, and the semi-transparent and semi-reflective film is configured to reflect the fluorescent light to an angle consistent with the direction of the imaging light path, and allow the reflected light of the measured sample to penetrate and be incident on the microscopic camera.
[0015] Preferably, a triangular prism is provided in the transmission channel, and the semi-transparent and semi-reflective film is arranged on the hypotenuse of the triangular prism.
[0016] Preferably, the white light bright field imaging module is configured such that in the fluorescence imaging mode, the bright field light source arranged under the transmission window is turned off.
[0017] Preferably, a grating is provided at the light outlet of the bright field light source, and the grating is configured to be opened in the white light bright field mode and closed in the fluorescence imaging mode.
[0018] Preferably, the panoramic imaging module further includes a white light backlight source, and the white light backlight source is disposed vertically opposite to the panoramic camera.
[0019] Compared with the prior art, the beneficial effects of the utility model include:
[0020] The utility model provides a multi-mode microscopic imaging system, which ensures the diversity of sample detection by setting three imaging modules at the same time. At the same time, the fluorescent molding module can change the optical detection scene from the white light field mode to the fluorescent imaging mode by adjusting the position of the fluorescent light source component; the panoramic imaging module only needs to move the sample to be tested from the bright field imaging position to the panoramic imaging position, and the optical detection scene can be changed from the white light field mode or the fluorescent imaging mode to the panoramic imaging mode. It is simple to operate, and there is no need to move the body of the sample to be tested, which avoids the problem that the image position observed by the optical microscope in different modes and the image position of the fluorescent microscope do not overlap and seriously affect the observation effect; and both can be controlled with high precision through computers or electrical modules to further ensure the detection accuracy.
[0021] The utility model arranges a fluorescent light source component to be inserted into or moved out of the imaging light path of the microscope imaging component through a position adjustment component. Specifically, a displacement track horizontally passes through the imaging light path between the objective lens and the stage; the fluorescent light source component is movably mounted on the displacement track through a slider, and the displacement track drives the fluorescent light source component to move into or out of the imaging light path of the microscope imaging component under the drive of a power module. In this way, when the white light field imaging mode is converted to the fluorescent imaging mode, the sample body, the microscopic camera, the objective lens, and the bright field light source do not undergo any displacement changes, thereby avoiding the problem that the image position observed by the optical microscope in different modes and the image position of the fluorescent microscope do not overlap, which seriously affects the observation effect.
[0022] The utility model sets a position sensor on the displacement track, and senses the distance between the current position of the fluorescent light source component and the end point of the displacement track through the position sensor. When the position sensor senses that the distance between the fluorescent light source component and the end point of the displacement track is the same as the track distance between the end point of the displacement track and the light-through channel, it can be determined that the fluorescent light source component has moved to the light-through channel. According to the preset position coordinates of the multiple fluorescent light source components, one of the multiple fluorescent light source components can be automatically adjusted to move to the light-through channel, and its transmission channel is coaxially arranged with the light-through channel, so that the microscopic camera can obtain different fluorescent reaction images. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 It is a schematic diagram of the three-dimensional structure of the multi-mode microscopic imaging system described in an embodiment of the utility model.
[0024] Figure 2 It is a schematic diagram of the three-dimensional structure of the microscope imaging component in the multi-mode microscopic imaging system described in an embodiment of the utility model.
[0025] Figure 3 It is another three-dimensional structural schematic diagram of the multi-mode microscopic imaging system described in an embodiment of the utility model.
[0026] Figure 4 It is a schematic diagram of the three-dimensional structure of the fluorescence imaging module in the multi-mode microscopic imaging system described in an embodiment of the utility model.
[0027] Figure 5 It is a three-dimensional structural schematic diagram of the fluorescent light source component in the multi-mode microscopic imaging system described in an embodiment of the utility model.
[0028] Figure 6 It is a schematic diagram of the three-dimensional structure of the panoramic imaging module in the multi-mode microscopic imaging system described in an embodiment of the utility model.
[0029] The markings of the components in the accompanying drawings are as follows:
[0030] 1. Microscope imaging component; 2. Fluorescent light source component; 3. Position adjustment component; 4. Panoramic camera; 5. Base; 6. Light source positioning base; 7. Light source protection cavity; 11. Microscopic camera; 12. Objective lens; 13. Storage table; 14. Bright field light source; 131. Storage slot; 21. Transmission channel; 22. Semi-transparent and semi-reflective film; 23. Triangular prism; 31. Displacement track; 32. Hydraulic buffer; 33. Rubber-coated screws; 34. Position sensor; 41. Fixed seat; 42. Upper light source; 43. White light backlight; 51. Horizontal base; 52. Longitudinal support; 71. Light channel. DETAILED DESCRIPTION
[0031] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.
[0032] In the prior art, different optical detection modes require manual disassembly and replacement of optical modules, which is not only cumbersome, but re-placing the sample will also cause the position and angle of the sample to change, resulting in the image position observed under an ordinary optical microscope and the image position under a fluorescence microscope not overlapping, seriously affecting the observation effect.
[0033] Based on this, the utility model provides a multi-mode microscopic imaging system, which has three imaging modes, namely white light bright field imaging mode, fluorescence imaging mode and panoramic imaging mode; based on the three imaging modes, the multi-mode microscopic imaging system is respectively provided with three groups of imaging modules, namely white light bright field imaging module, fluorescence imaging module and panoramic imaging module. Figure 1 and Figure 3 As shown, the white light field imaging module includes a microscope imaging component 1 and a bright field imaging position, and the white light field imaging module is configured to perform bright field imaging on the sample to be tested set at the bright field imaging position through the microscope imaging component 1. The fluorescence imaging component includes at least one fluorescent light source component 2 and a position adjustment component 3, and the fluorescent light source component 2 is configured to be inserted into the imaging light path of the microscope imaging component 1 or moved out of the imaging light path of the microscope imaging component 1 through the position adjustment component 3. The panoramic imaging module includes a panoramic camera 4 and a panoramic imaging position, and the panoramic camera 4 is configured to perform panoramic imaging on the sample to be tested set at the panoramic imaging position in a panoramic imaging mode. The multi-mode microscopic imaging system has three imaging modules at the same time, among which the fluorescent molding module can change the optical detection scene from the white light field mode to the fluorescent imaging mode only by adjusting the position of the fluorescent light source component 2; and the panoramic imaging module only needs to move the sample to be tested from the bright field imaging position to the panoramic imaging position to change the optical detection scene from the white light field mode or the fluorescent imaging mode to the panoramic imaging mode. The above operation is simple and does not require moving the sample body to be tested, thus avoiding the problem that the image position observed by the optical microscope in different modes and the image position of the fluorescence microscope do not overlap, which seriously affects the observation effect; and the above operations can all be controlled with high precision through a computer or electrical module to further ensure the detection accuracy.
[0034] It can be understood that the white light field imaging mode is an imaging mode in conventional microscopic detection, and the microscope imaging component 1 used therein is also similar to the composition structure of conventional microscopic imaging instruments, such as Figure 1 and Figure 2As shown, the microscope imaging assembly 1 includes a base 5, a micro camera 11, an objective lens 12, a stage 13 and a bright field light source 14. The base 5 includes a horizontal base 51 and a longitudinal support 52 perpendicular to the horizontal base 51. The stage 13 is arranged on the horizontal base 51. The objective lens 12 is fixedly connected to the longitudinal support 52 through a fixing set. The micro camera 11 is coaxially arranged on the objective lens 12, and its image acquisition end is connected to the rear lens end of the objective lens 12. The front lens end of the objective lens 12 is arranged toward the stage 13. The focus of the micro camera 11 and the focus of the objective lens 12 are also located on the same axis. A placement slot 131 with a transmission window is provided on the stage 13, which is used to limit the displacement of the slice of the sample to be tested; the objective lens 12 and the bright field light source 14 are relatively arranged at the upper and lower ends of the transmission window, and the micro camera 11, the objective lens 12, the transmission window and the bright field light source 14 are all located on the same axis. In the white light field imaging mode, the bright field light source 14 emits light from bottom to top, the light passes through the sample slice under test, and is incident on the image acquisition end of the microscopic camera 11 through the objective lens 12. In the white light field mode, the microscopic camera 11 can acquire a white light microscopic image of the sample under test.
[0035] It should be noted that, in order to ensure the detection accuracy, when switching from the white light field imaging mode to the fluorescent imaging mode, it is best to avoid moving the sample body to be tested, and also to avoid position changes of other imaging modules; therefore, in some preferred embodiments, the fluorescent light source component 2 is controlled by the position adjustment component 3 to be inserted into the imaging light path of the microscope imaging component 1 or to be removed from the imaging light path of the microscope imaging component 1. Figure 1 and Figure 4 As shown, the position adjustment component 3 includes a displacement track 31 that vertically passes through the imaging light path of the microscope imaging component 1. Specifically, the displacement track 31 horizontally passes through the imaging light path between the objective lens 12 and the stage 13; the fluorescent light source component 2 is movably mounted on the displacement track 31 through a slider, and the displacement track 31 drives the fluorescent light source component 2 to move into the imaging light path of the microscope imaging component 1 or out of the imaging light path of the microscope imaging component 1 under the drive of the power module. In this way, when the white light field imaging mode is converted to the fluorescent imaging mode, the sample body, the microscopic camera 11, the objective lens 12 and the bright field light source 14 do not undergo any displacement changes, thereby avoiding the problem that the image position observed by the optical microscope in different modes and the image position of the fluorescent microscope do not overlap and seriously affect the observation effect.
[0036] When the fluorescent light source component 2 is inserted into the imaging light path of the microscope imaging assembly 1, it is necessary not only to ensure that the fluorescent light is projected onto the sample to be tested along the imaging light path, but also to ensure that the fluorescent light reflected by the sample can pass through the fluorescent light source component 2 and be incident on the image acquisition end of the microscopic camera 11 through the objective lens 12. Figure 4 and Figure 5As shown, in some preferred embodiments, a transmission channel 21 is provided in the fluorescent light source component 2, and a semi-transparent and semi-reflective film 22 is provided in the transmission channel 21. When the fluorescent light source component 2 is inserted into the imaging light path of the microscope imaging assembly 1, the transmission channel 21 is coaxially arranged with the imaging light path, and the semi-transparent and semi-reflective film 22 provided in the transmission channel 21 reflects the fluorescent light to an angle consistent with the direction of the imaging light path, and allows the reflected fluorescence to penetrate and be incident on the camera. In some more preferred embodiments, a triangular prism 23 is provided in the transmission channel 21, and the semi-transparent and semi-reflective film 22 is provided on the hypotenuse of the triangular prism 23; without affecting the optical performance of the semi-transparent and semi-reflective film 22, a stable angle support is provided for it.
[0037] Based on the different fluorescence detection effects of various components in the sample to be tested, it is necessary to perform multiple fluorescence reaction detections on the sample to be tested during the detection of the sample to be tested. In some preferred embodiments, a plurality of fluorescent light source components 2 can be movably mounted on the displacement track 31. In the panoramic imaging mode, the displacement track 31 is configured to drive the plurality of fluorescent light source components 2 to move, so that one of the plurality of fluorescent light source components 2 is inserted into the imaging light path of the microscope imaging component 1, and its transmission channel 21 is coaxially arranged with the imaging light path. The light outlet of the fluorescent light source component 2 inserted into the imaging light path of the microscope imaging component 1 is located on the same axis as the camera, objective lens 12, transmission window and bright field light source 14. Specifically, Figure 4 and Figure 5 As shown, the position adjustment assembly 3 includes a light source positioning base 6, a light source protection cavity 7, and a displacement track 31, wherein one end of the light source positioning base 6 is fixed on the longitudinal support 52, and the other end extends horizontally and vertically passes through the imaging light path between the objective lens 12 and the placement table 13, and the light source protection cavity 7 is fixed on the light source positioning base 6, one end of which extends into the imaging light path between the objective lens 12 and the placement table 13, and a light passage 71 is provided relative to the imaging light path. Two parallel displacement tracks 31 are laid on one side of the light source protection cavity 7, and one end of the multiple fluorescent light source components 2 are simultaneously mounted on the parallel displacement tracks 31 through a slider, and the other end extends into the light source protection cavity 7. When one of the multiple fluorescent light source components 2 is driven by the power component of the displacement track 31 to move into the light channel 71 and make its transmission channel 21 coaxially arranged with the light channel 71, at this time, the fluorescent light emitted by the fluorescent light source component 2 is reflected by the semi-transparent and semi-reflective film 22 and projected onto the slice of the sample to be tested along the imaging light path direction, the cells of the sample to be tested produce a fluorescent reaction, and the fluorescent response light is reflected by the semi-transparent and semi-reflective film 22 to the microscopic camera 11, so that the microscopic camera 11 collects the corresponding sample fluorescence reaction image.
[0038] In some preferred embodiments, both sides of the displacement track 31 are provided with limit buffer components, which are used to limit the displacement path of the fluorescent light source component 2 from exceeding the displacement stroke of the displacement track 31, and at the same time, buffer the fluorescent light source component 2 that runs to the end of the displacement track 31, and use a flexible buffering method to stop the fluorescent light source component 2 from continuing to move. While preventing the displacement path of the fluorescent light source component 2 from exceeding the displacement stroke of the displacement track 31, it also avoids rigid impact on the fluorescent light source component 2, minimizes the vibration impact on the fluorescent light source component 2, and ensures the service life of the fluorescent light source component 2. Figure 4 As shown, in some preferred embodiments, the limit buffer component includes an oil pressure buffer 32 and a rubber-coated screw 33 provided on both sides of the displacement track 31, wherein the oil pressure buffer 32 is set between the parallel displacement tracks 31, and performs flexible limit buffering on the body of the fluorescent light source component 2, thereby realizing a first-level limit buffering; the rubber-coated screw 33 is set in the gap between the parallel displacement tracks 31, and performs buffering and limit on the slider below the fluorescent light source component 2, thereby realizing a second-level limit buffering. In addition, the oil pressure buffer 32 can also be connected to the central control module for communication. When the oil pressure buffer 32 senses the abutment pressure of the fluorescent light source component 2, it means that the fluorescent light source component 2 is reset to the end of the travel of the displacement track 31, thereby timely locking the current position of the fluorescent light source component 2, and providing a positioning basis for its subsequent displacement control.
[0039] For the displacement control of the fluorescent light source component 2, a panoramic coordinate system can be pre-designed, and the displacement stroke of the fluorescent light source component 2 can be set by the position coordinates of the fixed components; for example, the position coordinates of the stroke end point of the displacement track 31 and the position coordinates of the light passage 71 are both fixed coordinates, and the distance between them is also a fixed distance. Therefore, when the fluorescent light source component 2 is reset to the stroke end point of the displacement track 31, based on the track distance between the stroke end point of the displacement track 31 and the light passage 71, the fluorescent light source component 2 is controlled to move a preset distance along the displacement track 31 to the bottom of the light passage 71. In addition, a position sensor 34 can also be set on the displacement track 31, and the position sensor 34 senses the distance between the current position of the fluorescent light source component 2 and the stroke end point of the displacement track 31. When the position sensor 34 senses that the distance between the fluorescent light source component 2 and the stroke end point of the displacement track 31 is the same as the track distance between the stroke end point of the displacement track 31 and the light passage 71, it can be determined that the fluorescent light source component 2 has moved to the bottom of the light passage 71. According to the preset position coordinates of the multiple fluorescent light source components 2, one of the multiple fluorescent light source components 2 can be automatically adjusted to move into the light channel 71, and its transmission channel 21 is coaxially arranged with the light channel 71, so that the microscopic camera 11 can obtain different fluorescent reaction images.
[0040] In order to prevent the bright field light source 14 from interfering with the low-light-level camera in collecting the fluorescence reaction image in the fluorescence imaging mode, the bright field light source 14 set under the transmission window of the white light field imaging module is turned off in the fluorescence imaging mode. A light shielding component can also be used to block the light of the bright field light source 14. In some preferred embodiments, the light shielding component can be set between the bright field light source 14 and the transmission window. When performing fluorescence imaging, the light shielding component is unfolded to block the light emitted by the bright field light source 14 to the direction of the transmission window. When performing white light field imaging, the light shielding component is retracted to project the light of the bright field light source 14 to the direction of the transmission window. The light shielding component can be any component that can achieve the light shielding function in the prior art. The expansion and contraction of the light shielding component can also be achieved by displacement or stretching and folding. In some preferred embodiments, a grating is provided at the light outlet of the bright field light source 14, and the grating is configured to be opened in the white light field mode and closed in the fluorescence imaging mode; that is, the grating is used as a light shielding component to prevent the bright field light source 14 from interfering with the low-light-level camera in collecting the fluorescence reaction image in the fluorescence imaging mode.
[0041] To minimize interference with white light bright field mode or fluorescence imaging mode, such as Figure 6 As shown, the panoramic camera 4 is arranged on one side of the microscope imaging assembly 1 through a fixed seat 41, and is arranged relative to the panoramic imaging position; the bright field imaging position and the panoramic imaging position are both arranged on the horizontal base 51. In the white light field mode or the fluorescence imaging mode, the stage 13 is arranged at the bright field imaging position. When it is necessary to switch to the panoramic imaging mode, the stage 13 needs to be moved from the bright field imaging position to the panoramic imaging position. In some preferred embodiments, a moving platform is provided below the stage 13, and the stage 13 is driven by the power component of the moving platform to change its position between the bright field imaging position and the panoramic imaging position. When it is necessary to switch to the white light field mode or the fluorescence imaging mode, the stage 13 is driven by the power component of the moving platform to move to the bright field imaging position, so that the microscopic camera 11, the objective lens 12, the transmission window and the bright field light source 14 are all located on the same axis; when it is necessary to switch to the panoramic imaging mode, the stage 13 is driven by the power component of the moving platform to move to the panoramic imaging position, so that the storage slot 131 and the panoramic camera 4 are arranged relative to each other up and down. To ensure that the panoramic image captured by the panoramic camera 4 is clearer, in some more preferred embodiments, the panoramic imaging module also includes upper and lower light sources, wherein the upper light source 42 is arranged on one side of the panoramic camera 4 to provide upper light for the panoramic imaging of the sample under test; the lower light source is a white light backlight source 43 arranged at the panoramic imaging position, which is arranged opposite to the panoramic camera 4 up and down to provide sufficient backlight for the panoramic imaging of the sample under test.
[0042] In summary, the multi-mode microscopic imaging system provided by the embodiment of the utility model ensures the diversity of sample detection by setting three imaging modules at the same time. At the same time, the fluorescent molding module can change the optical detection scene from the white light field mode to the fluorescent imaging mode only by adjusting the position of the fluorescent light source component 2; the panoramic imaging module only needs to move the sample to be tested from the bright field imaging position to the panoramic imaging position, so that the optical detection scene can be changed from the white light field mode or the fluorescent imaging mode to the panoramic imaging mode. It is simple to operate, and there is no need to move the body of the sample to be tested, which avoids the problem that the image position observed by the optical microscope in different modes and the image position of the fluorescent microscope do not overlap and seriously affect the observation effect; and both can be controlled with high precision through computers or electrical modules to further ensure the detection accuracy.
[0043] The above are only preferred embodiments of the present invention, and are not intended to limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not intended to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.
Claims
1. A multi-mode microscopic imaging system, characterized in that: include: A white light bright field imaging module, the white light bright field imaging module comprising a microscope imaging component and a bright field imaging position, the white light bright field imaging module being configured to perform bright field imaging of a sample to be tested disposed at the bright field imaging position through the microscope imaging component; A fluorescence imaging module, the fluorescence imaging module comprising at least one fluorescence light source component and a position adjustment component, the fluorescence light source component being configured to be inserted into or removed from the imaging light path of the microscope imaging component through the position adjustment component; A panoramic imaging module, the panoramic imaging module includes a panoramic camera and a panoramic imaging position, the panoramic camera is configured to perform panoramic imaging of a sample to be tested set at the panoramic imaging position in a panoramic imaging mode.
2. The multi-mode microscopic imaging system according to claim 1, characterized in that: The microscope imaging assembly includes a microscopic camera, an objective lens, a stage and a bright field light source. The microscopic camera and the objective lens are arranged on the same axis, and the focus of the microscopic camera and the focus of the objective lens are also located on the same axis.
3. The multi-mode microscopic imaging system according to claim 2, characterized in that: The platform is provided with a storage slot with a transmission window, the objective lens and the bright field light source are arranged at the upper and lower ends of the transmission window respectively, and the microscopic camera, the objective lens, the transmission window and the bright field light source are all located on the same axis.
4. The multi-mode microscopic imaging system according to claim 1, characterized in that: The position adjustment component includes a displacement track that vertically passes through the imaging light path of the microscope imaging component. The fluorescent light source component is movably mounted on the displacement track through a slider. The displacement track is configured to drive the fluorescent light source component to move into the imaging light path of the microscope imaging component or move out of the imaging light path of the microscope imaging component.
5. The multi-mode microscopic imaging system according to claim 4, characterized in that: The displacement track is movable with a plurality of fluorescent light source components. In the panoramic imaging mode, the displacement track is configured to drive the plurality of fluorescent light source components to move so that one of the plurality of fluorescent light source components is inserted into the imaging light path of the microscope imaging assembly.
6. The multi-mode microscopic imaging system according to claim 1, characterized in that: A transmission channel is provided in the fluorescent light source component, and the transmission channel is configured so that when the fluorescent light source component is inserted into the imaging light path of the microscope imaging assembly, the transmission channel is coaxially arranged with the imaging light path; a semi-transparent and semi-reflective film is provided in the transmission channel, and the semi-transparent and semi-reflective film is configured to reflect the fluorescent light to an angle consistent with the direction of the imaging light path, and allow the reflected light of the measured sample to penetrate and be incident on the microscopic camera.
7. The multi-mode microscopic imaging system according to claim 6, characterized in that: A triangular prism is arranged in the transmission channel, and the semi-transparent and semi-reflective sheet is arranged on the hypotenuse of the triangular prism.
8. The multi-mode microscopic imaging system according to claim 1, characterized in that: The white light bright field imaging module is configured such that in the fluorescence imaging mode, the bright field light source arranged under the transmission window is turned off.
9. The multi-mode microscopic imaging system according to claim 8, characterized in that: A grating is provided at the light outlet of the bright field light source, and the grating is configured to be opened in a white light bright field mode and closed in a fluorescence imaging mode.
10. The multi-mode microscopic imaging system according to claim 1, characterized in that: The panoramic imaging module also includes a white light backlight source, and the white light backlight source is arranged vertically opposite to the panoramic camera.