Multi-mode fluorescence imaging mechanism
By automatically replacing the fluorescent light source components with position adjustment components in fluorescence microscopes, the problems of cumbersome operation and change of sample position angle caused by repeated replacement of fluorescent modules in the prior art are solved, and efficient and accurate multi-mode fluorescence imaging is achieved.
Patent Information
- Application Number
- CN202421665056.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-12
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2034-07-12
AI Technical Summary
When switching between various fluorescence imaging modes, existing fluorescence microscopes need to repeatedly replace the fluorescence module, which is complicated to operate and affect the position and angle of the sample, resulting in low detection efficiency and poor observation effect.
A multi-mode fluorescence imaging mechanism is designed, and a position adjustment component is used to control multiple fluorescence light source components to automatically alternately enter the imaging optical path of the microscope imaging component to achieve high-precision light source position adjustment.
The detection efficiency and accuracy of multi-fluorescence imaging of samples are improved, the changes in sample position and angle are avoided, and the stability of the observation effect is ensured.
Smart Images
Figure CN222952542U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of fluorescence imaging, in particular to a multi-mode fluorescence imaging mechanism. Background Art
[0002] Currently, microscopes are divided into two categories, namely fluorescence microscopes and ordinary optical microscopes. Ordinary optical microscopes observe samples with ordinary light sources, and can image samples in color and black and white. Unlike ordinary optical microscopes, fluorescence microscopes use light of a certain wavelength (ultraviolet light, blue-violet light) to excite the fluorescent substances in the specimens under the microscope to emit fluorescence. The role of the light source of a fluorescence microscope is not direct illumination, but as an energy source to excite the fluorescent substances in the specimens. The specimens are observed through the fluorescence phenomenon presented by the fluorescent substances in the specimens after absorbing the excitation light energy. The light source of a fluorescence microscope can supply a large amount of excitation light in a specific wavelength range, so that the fluorescent substances in the specimens under examination can obtain the necessary intensity of excitation light.
[0003] After observing and measuring the fluorescence pattern of a sample through a fluorescence microscope, users often need to observe reaction images of other different colors of fluorescence, which requires replacing the fluorescence module in the imaging system. When performing cell imaging on a large number of samples, repeated replacement of the fluorescence module will seriously affect the sample detection efficiency. It may also cause the position and angle of the sample to change during the operation, seriously affecting the observation effect.
[0004] Therefore, a microscopic imaging mechanism having multiple fluorescence imaging modes at the same time is very necessary. Utility Model Content
[0005] The purpose of the utility model is to overcome the above technical deficiencies and provide a multi-mode fluorescence imaging mechanism, which is simple to operate, has high efficiency in multi-fluorescence imaging detection of samples, and avoids the problem that the position and angle of the sample are changed, which seriously affects the observation effect.
[0006] In order to achieve the above-mentioned technical objectives, the technical solution of the utility model provides a single-channel sample sorting device, which includes: a microscope imaging component, a plurality of fluorescent light source components and a position adjustment component, the microscope imaging component has an imaging light path, and the plurality of fluorescent light source components are arranged on the position adjustment component, and the position adjustment component is configured to drive the plurality of fluorescent light source components to be inserted into the imaging light path of the microscope imaging component or to be moved out of the imaging light path of the microscope imaging component, and to make one of the plurality of fluorescent light source components enter the imaging light path of the microscope imaging component.
[0007] Preferably, the microscope imaging assembly includes a microscopic camera, an objective lens and a stage, the microscopic camera and the objective lens are arranged on the same axis, and the focal points of the microscopic camera and the objective lens also fall on the same axis.
[0008] Preferably, the position adjustment component includes a displacement track that vertically passes through the imaging light path of the microscope imaging component, and the fluorescent light source component is movably mounted on the displacement track through a slider, and the displacement track is configured to drive the fluorescent light source component to move into the imaging light path of the microscope imaging component or move out of the imaging light path of the microscope imaging component.
[0009] Preferably, the displacement track vertically passes through the displacement track of the imaging light path of the microscope imaging assembly.
[0010] Preferably, both sides of the displacement guide rail are provided with limit buffer components for limiting the displacement path of the fluorescent light source component from exceeding the displacement stroke of the displacement guide rail.
[0011] Preferably, the limit buffer component includes a hydraulic buffer and a rubber-coated screw provided on both sides of the displacement guide rail, wherein the hydraulic buffer is mounted between the parallel displacement guide rails, and the rubber-coated screw is arranged in the gap between the parallel displacement guide rails.
[0012] Preferably, a position sensor is provided at the end point of the displacement track for sensing the distance between the current position of the fluorescent light source component and the end point of the displacement track.
[0013] Preferably, a transmission channel is provided in the fluorescent light source component, and the transmission channel is configured so that when the fluorescent light source component is inserted into the imaging light path of the microscope imaging component, the transmission channel is coaxially arranged with the imaging light path of the microscope imaging component.
[0014] Preferably, a semi-transparent and semi-reflective film is provided in the transmission channel, and the semi-transparent and semi-reflective film is configured to reflect the fluorescent light to an angle consistent with the direction of the imaging light path, and to allow the reflected light of the measured sample to penetrate and be incident on the microscopic camera.
[0015] Preferably, a triangular prism is provided in the transmission channel, and the semi-transparent and semi-reflective film is arranged on the hypotenuse of the triangular prism.
[0016] Compared with the prior art, the beneficial effects of the utility model include:
[0017] The utility model provides a multi-mode fluorescence imaging mechanism, in which, under the control of the position adjustment component, multiple fluorescent light source components can automatically realize position adjustment, and are alternately replaced to enter the imaging light path of the microscope imaging component, and the operation is simple and convenient, and can realize high-precision control through a computer or electrical module, which greatly improves the efficiency and accuracy of sample multi-fluorescence imaging detection. In addition, during the process of replacing the fluorescent light source, only the fluorescent light source component undergoes position change, avoiding affecting the observation effect of the measured sample due to the position and angle of the measured sample being affected.
[0018] The fluorescent light source component of the utility model is mounted on the storage table through a position adjustment component, and there is no contact between the fluorescent light source component and the storage table. Therefore, during the replacement of the fluorescent light source, the position adjustment component drives the fluorescent light source component to change its position, and will not produce any contact effect on the sample to be tested placed on the storage table, that is, it will not change the position and angle of the sample to be tested, thereby avoiding any impact on the observation effect.
[0019] The utility model sets a position sensor on the displacement track, and senses the distance between the current position of the fluorescent light source component and the end point of the displacement track through the position sensor. When the position sensor senses that the distance between the fluorescent light source component and the end point of the displacement track is the same as the track distance between the end point of the displacement track and the light-through channel, it can be determined that the fluorescent light source component has moved to the light-through channel. According to the preset position coordinates of the multiple fluorescent light source components, one of the multiple fluorescent light source components can be automatically adjusted to move to the light-through channel, and its transmission channel is coaxially arranged with the light-through channel, so that the microscopic camera can obtain different fluorescent reaction images. BRIEF DESCRIPTION OF THE DRAWINGS
[0020] Figure 1 It is a three-dimensional structural schematic diagram of the multi-mode fluorescence imaging mechanism described in an embodiment of the utility model.
[0021] Figure 2 It is a three-dimensional structural schematic diagram of a fluorescent light source component in a multi-mode fluorescent imaging mechanism described in an embodiment of the utility model, which is arranged on a position adjustment component.
[0022] Figure 3 It is a three-dimensional structural schematic diagram of a fluorescent light source component in a multi-mode fluorescent imaging mechanism according to an embodiment of the utility model;
[0023] Figure 4 It is a three-dimensional structural schematic diagram of a microscope imaging component in a multi-mode fluorescence imaging mechanism described in an embodiment of the utility model.
[0024] The markings of the components in the accompanying drawings are as follows:
[0025] 1. Microscope imaging component; 2. Fluorescent light source component; 3. Position adjustment component; 4. Base; 11. Micro camera; 12. Objective lens; 13. Storage table; 131. Storage slot; 21. Transmission channel; 22. Semi-transparent and semi-reflective film; 23. Triangular prism; 31. Light source positioning base; 32. Light source protection cavity; 33. Displacement track; 321. Light channel; 34. Hydraulic buffer; 35. Rubber-coated screws; 36. Position sensor; 41. Horizontal base; 42. Longitudinal support. DETAILED DESCRIPTION
[0026] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.
[0027] Based on the different fluorescence detection effects of various components in the sample being tested, it is necessary to perform multiple fluorescence reaction tests on the sample being tested during the detection process. In the prior art, when performing multiple fluorescence reaction detection imaging on a large number of samples, it is necessary to repeatedly replace the fluorescence module, which is cumbersome and seriously affects the sample detection efficiency. It is also possible that the position and angle of the sample will change during the operation, which will seriously affect the observation effect.
[0028] Based on this, the utility model provides a multi-mode fluorescence imaging mechanism, such as Figure 1-4 As shown, it includes a microscope imaging component 1, a plurality of fluorescent light source components 2 and a position adjustment component 3. The microscope imaging component 1 has an imaging light path. The plurality of fluorescent light source components 2 are all arranged on the position adjustment component 3. The position adjustment component 3 is configured to drive the plurality of fluorescent light source components 2 to be inserted into the imaging light path of the microscope imaging component 1 or to be removed from the imaging light path of the microscope imaging component 1, and to make one of the plurality of fluorescent light source components 2 enter the imaging light path of the microscope imaging component 1. Under the control of the position adjustment component 3, the plurality of fluorescent light source components 2 can automatically realize position adjustment and alternately replace and enter the imaging light path of the microscope imaging component 1. The operation is simple and convenient, and high-precision control can be realized through a computer or an electrical module, which greatly improves the efficiency and accuracy of the multi-fluorescence imaging detection of the sample. In addition, in the process of replacing the fluorescent light source, only the fluorescent light source component 2 changes its position, so as to avoid affecting the observation effect of the measured sample due to the position and angle of the measured sample being affected.
[0029] It is understandable that the microscope imaging assembly 1 can adopt any mechanism or device that can realize microscopic imaging in the existing microscopic imaging system. Figure 1 and Figure 2As shown, the microscope imaging assembly 1 includes a base 4, a micro camera 11, an objective lens 12 and a stage 13. The base 4 includes a horizontal base 41 and a longitudinal support 42 perpendicular to the horizontal base 41. The stage 13 is arranged on the horizontal base 41. The objective lens 12 is fixedly connected to the longitudinal support 42 through a fixing set. The micro camera 11 is coaxially arranged on the objective lens 12, and its image acquisition end is connected to the rear lens end of the objective lens 12. The front lens end of the objective lens 12 is arranged toward the stage 13. The focal points of the micro camera 11 and the objective lens 12 also fall on the same axis. The stage 13 is provided with a placement slot 131 for limiting the displacement of the slice of the sample to be tested.
[0030] It should be noted that, in order to ensure the detection accuracy, when switching between different fluorescence imaging modes, it is best to avoid moving the sample body to be tested; in some preferred embodiments, the plurality of fluorescence light source components 2 are controlled by the position adjustment component 3 to be inserted into or removed from the imaging light path of the microscope imaging component 1. Figure 2 and Figure 4 As shown, the position adjustment component 3 includes a displacement track 33 that vertically passes through the imaging light path of the microscope imaging component 1. Specifically, the displacement track 33 horizontally passes through the imaging light path between the objective lens 12 and the stage 13; multiple fluorescent light source components 2 are movably mounted on the displacement track 33 through a slider. Driven by the power module, the displacement track 33 drives the fluorescent light source components 2 to move into or out of the imaging light path of the microscope imaging component 1, and at the current moment, only one of the multiple fluorescent light source components 2 enters the imaging light path of the microscope imaging component 1. Figure 2 As shown, the fluorescent light source component 2 is mounted on the storage table 13 through the position adjustment component 3, and there is no contact between it and the storage table 13. Therefore, during the replacement of the fluorescent light source, the position adjustment component 3 drives the fluorescent light source component 2 to change its position, and will not have any contact effect on the sample to be tested placed on the storage table 13, that is, it will not change the position and angle of the sample to be tested, thereby avoiding any impact on the observation effect.
[0031] The light outlet of the fluorescent light source component 2 inserted into the imaging light path of the microscope imaging assembly 1 is located on the same axis as the camera, the objective lens 12, the transmission window and the bright field light source. Figure 2As shown, in some preferred embodiments, the position adjustment component 3 includes a light source positioning base 31, a light source protection cavity 32, a displacement track 33, a plurality of fluorescent light source components 2, and a limit buffer component, wherein one end of the light source positioning base 31 is fixed on the longitudinal support 42, and the other end extends horizontally and vertically passes through the imaging light path between the objective lens 12 and the placement table 13, and the light source protection cavity 32 is fixed on the light source positioning base 31, one end of which is set in the imaging light path between the objective lens 12 and the placement table 13, and a light passage 321 is provided relative to the imaging light path. Two parallel displacement guide rails are laid on one side of the light source protection cavity 32, and one end of the plurality of fluorescent light source components 2 is simultaneously mounted on the parallel displacement guide rail through a slider, and the other end extends into the light source protection cavity 32. When one of the multiple fluorescent light source components 2 is driven by the power component of the displacement guide to move into the light channel 321 and make its transmission channel 21 coaxial with the light channel 321, at this time, the fluorescent light emitted by the fluorescent light source component 2 is reflected by the semi-transparent and semi-reflective film 22 and projected onto the slice of the sample to be tested along the imaging light path direction, the cells of the sample to be tested produce a fluorescent reaction, and the fluorescent response light is reflected by the semi-transparent and semi-reflective film 22 to the microscopic camera 11, so that the microscopic camera 11 collects the corresponding sample fluorescence reaction image.
[0032] In some more preferred embodiments, both sides of the displacement guide rail are provided with limit buffer components, which are used to limit the displacement path of the fluorescent light source component 2 from exceeding the displacement stroke of the displacement guide rail, and at the same time, buffer the fluorescent light source component 2 that runs to the end of the displacement guide rail, and use a flexible buffering method to stop the fluorescent light source component 2 from continuing to move. While preventing the displacement path of the fluorescent light source component 2 from exceeding the displacement stroke of the displacement guide rail, it also avoids rigid impact on the fluorescent light source component 2, minimizes the vibration impact on the fluorescent light source component 2, and ensures the service life of the fluorescent light source component 2. In some more preferred embodiments, the limit buffer component includes an oil pressure buffer 34 and a rubber-coated screw 35 provided on both sides of the displacement guide rail, wherein the oil pressure buffer 34 is mounted between the parallel displacement guide rails, and performs flexible limit buffering on the body of the fluorescent light source component 2 to achieve a first-level limit buffer; the rubber-coated screw 35 is set in the gap between the parallel displacement guide rails, and performs buffering and limit on the slider below the fluorescent light source component 2 to achieve a second-level limit buffer. In addition, the oil pressure buffer 34 can also be connected to the central control module for communication. When the oil pressure buffer 34 senses the abutment pressure of the fluorescent light source component 2, it means that the fluorescent light source component 2 is reset to the end of the travel of the displacement track 33, thereby timely locking the current position of the fluorescent light source component 2 and providing a positioning basis for its subsequent displacement control.
[0033] For the displacement control of the fluorescent light source component 2, a panoramic coordinate system can be pre-designed, and the displacement stroke of the fluorescent light source component 2 can be set by the position coordinates of the fixed components; for example, the position coordinates of the end point of the displacement track 33 and the light passage 321 are both fixed coordinates, and the distance between them is also a fixed distance. Therefore, when the fluorescent light source component 2 is reset to the end point of the displacement track 33, based on the track distance between the end point of the displacement track 33 and the light passage 321, the fluorescent light source component 2 is controlled to move a preset distance along the displacement track 33 to the bottom of the light passage 321. In addition, a position sensor 36 can also be set on the displacement track 33, and the position sensor 36 senses the distance between the current position of the fluorescent light source component 2 and the end point of the displacement track 33. When the position sensor 36 senses that the distance between the fluorescent light source component 2 and the end point of the displacement track 33 is the same as the track distance between the end point of the displacement track 33 and the light passage 321, it can be determined that the fluorescent light source component 2 has moved to the bottom of the light passage 321. According to the preset position coordinates of the multiple fluorescent light source components 2, one of the multiple fluorescent light source components 2 can be automatically adjusted to move into the light channel 321, and its transmission channel 21 is coaxially arranged with the light channel 321, so that the microscopic camera 11 can obtain different fluorescent reaction images.
[0034] When the fluorescent light source component 2 is inserted into the imaging light path of the microscope imaging assembly 1, it is necessary not only to ensure that the fluorescent light is projected onto the sample to be tested along the imaging light path, but also to ensure that the fluorescent light reflected by the sample can pass through the fluorescent light source component 2 and be incident on the image acquisition end of the microscopic camera 11 through the objective lens 12. Figure 3 As shown, in some preferred embodiments, a transmission channel 21 is provided in the fluorescent light source component 2, and a semi-transparent and semi-reflective film 22 is provided in the transmission channel 21. When the fluorescent light source component 2 is inserted into the imaging light path of the microscope imaging assembly 1, the transmission channel 21 is coaxially arranged with the imaging light path, and the semi-transparent and semi-reflective film 22 provided in the transmission channel 21 reflects the fluorescent light to an angle consistent with the direction of the imaging light path, and allows the reflected fluorescence to penetrate and be incident on the camera. In some more preferred embodiments, a triangular prism 23 is provided in the transmission channel 21, and the semi-transparent and semi-reflective film 22 is provided on the hypotenuse of the triangular prism 23; without affecting the optical performance of the semi-transparent and semi-reflective film 22, a stable angle support is provided for it.
[0035] In summary, the embodiment of the utility model provides a multi-mode fluorescence imaging mechanism, in which, under the control of the position adjustment component 3, multiple fluorescent light source components 2 can automatically adjust their positions and alternately enter the imaging light path of the microscope imaging component 1. The operation is simple and convenient, and high-precision control can be achieved through a computer or electrical module, which greatly improves the efficiency and accuracy of sample multi-fluorescence imaging detection. In addition, during the replacement of the fluorescent light source, only the fluorescent light source component 2 changes its position, avoiding affecting the observation effect of the sample being tested due to the position and angle of the sample being tested being affected.
[0036] The above are only preferred embodiments of the present invention, and are not intended to limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not intended to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.
Claims
1. A multi-mode fluorescence imaging mechanism, characterized in that: include: A microscope imaging component, a plurality of fluorescent light source components and a position adjustment component, wherein the microscope imaging component has an imaging light path, the plurality of fluorescent light source components are arranged on the position adjustment component, and the position adjustment component is configured to drive the plurality of fluorescent light source components to be inserted into or removed from the imaging light path of the microscope imaging component, and to enable one of the plurality of fluorescent light source components to enter the imaging light path of the microscope imaging component.
2. The multi-mode fluorescence imaging mechanism according to claim 1, characterized in that: The microscope imaging component includes a microscopic camera, an objective lens and a stage. The microscopic camera and the objective lens are arranged on the same axis, and the focal points of the microscopic camera and the objective lens also fall on the same axis.
3. The multi-mode fluorescence imaging mechanism according to claim 1, characterized in that: The position adjustment component includes a displacement track that vertically passes through the imaging light path of the microscope imaging component. The fluorescent light source component is movably mounted on the displacement track through a slider. The displacement track is configured to drive the fluorescent light source component to move into the imaging light path of the microscope imaging component or move out of the imaging light path of the microscope imaging component.
4. The multi-mode fluorescence imaging mechanism according to claim 3, characterized in that: The displacement track vertically passes through the displacement track of the imaging light path of the microscope imaging component.
5. The multi-mode fluorescence imaging mechanism according to claim 3, characterized in that: Position limiting buffer components are provided on both sides of the displacement track, which are used to limit the displacement path of the fluorescent light source component from exceeding the displacement stroke of the displacement track.
6. The multi-mode fluorescence imaging mechanism according to claim 5, characterized in that: The limit buffer component includes a hydraulic buffer and a rubber-coated screw provided on both sides of the displacement track, wherein the hydraulic buffer is mounted between the parallel displacement tracks, and the rubber-coated screw is arranged in the gap between the parallel displacement tracks.
7. The multi-mode fluorescence imaging mechanism according to claim 3, characterized in that: A position sensor is provided at the end point of the displacement track for sensing the distance between the current position of the fluorescent light source component and the end point of the displacement track.
8. The multi-mode fluorescence imaging mechanism according to claim 1, characterized in that: A transmission channel is provided in the fluorescent light source component, and the transmission channel is configured so that when the fluorescent light source component is inserted into the imaging light path of the microscope imaging component, the transmission channel is coaxially arranged with the imaging light path of the microscope imaging component.
9. The multi-mode fluorescence imaging mechanism according to claim 8, characterized in that: A semi-transparent and semi-reflective sheet is arranged in the transmission channel, and the semi-transparent and semi-reflective sheet is configured to reflect the fluorescent light to an angle consistent with the direction of the imaging light path, and to allow the reflected light of the measured sample to penetrate and be incident on the microscopic camera.
10. The multi-mode fluorescence imaging mechanism according to claim 9, characterized in that: A triangular prism is arranged in the transmission channel, and the semi-transparent and semi-reflective sheet is arranged on the hypotenuse of the triangular prism.