Antibody neutralizing and mixing device

Abstract

The utility model discloses an antibody neutralizing and mixing device which comprises an antibody container, a neutralizing liquid container, metering pumps, a Y mixer and a reactor, the liquid inlet ends of the two metering pumps are respectively connected with the antibody container and the neutralizing liquid container, and the liquid outlet ends of the two metering pumps are communicated with two ports of the Y mixer; the rest port of the Y mixer is communicated with the reactor; and a spiral blade is arranged in the inner cavity of the reactor. The reactor is good in using effect, the flowing time of mixed liquid is prolonged through the spiral blades, the neutralizing effect is guaranteed, the mixed liquid in the reactor can be heated by heating the liquid, and therefore the purpose of neutralizing is achieved in an accelerated mode.

Description

Technical Field

[0001] The utility model relates to an antibody neutralization mixing device. Background Technique

[0002] In the existing affinity chromatography sample collection process, due to the low pH environment of the affinity chromatography elution conditions, there is a certain hydrolysis effect on antibodies, and antibodies are unstable in this environment, with the risk of flocculation and denaturation. However, during the pH adjustment process after sample collection, the antibodies will become turbid and a large amount of precipitation will occur, resulting in instability. Summary of the Invention

[0003] The utility model provides an antibody neutralization mixing device to solve the above problems existing in the prior art. According to the actual needs and the mixing ratio required, the two metering pumps are started at different speeds, and then enter the spiral reactor through the Y mixer until the neutralization reaction ends, and then the sample is collected.

[0004] The technical solution adopted by the utility model is as follows:

[0005] An antibody neutralization mixing device includes an antibody container, a neutralizing solution container, metering pumps, a Y mixer and a reactor. The liquid inlet ends of the two metering pumps are respectively connected to the antibody container and the neutralizing solution container. The liquid outlet ends of the two metering pumps are connected to two of the ports of the Y mixer, and the remaining port of the Y mixer is connected to the reactor. A spiral blade is arranged in the inner cavity of the reactor.

[0006] Further, a hollow tube is arranged at the axis of the inner cavity of the reactor. An installation hole is arranged at the center of the spiral blade, and the installation hole is fixedly connected to the hollow tube. A heating flow channel is arranged inside the side surface of the reactor, and the heating flow channel is communicated with the inner cavity of the hollow tube.

[0007] Further, the outer contour surface of the spiral blade fits with the inner cavity wall of the reactor.

[0008] Further, the reactor has a structure with a closed upper end and an open lower end. A connecting pipe is arranged on the upper end surface of the reactor, and the connecting pipe is connected to the Y mixer. A filter screen is fixed on the open lower end surface of the reactor.

[0009] The utility model has the following beneficial effects:

[0010] The utility model has good use effect. The spiral blade lengthens the flow time of the mixed liquid, ensures the neutralization effect, and the mixed liquid in the reactor can be heated by heating the liquid in the reactor, so as to accelerate the achievement of the neutralization purpose. Brief Description of the Drawings

[0011] Figure 1 It is a structural diagram of the utility model.

[0012] Figure 2 It is a structural diagram of a reactor. Specific implementation manners

[0013] The present utility model will be further described below in conjunction with the accompanying drawings.

[0014] As Figure 1 shown, an antibody neutralization mixing device of the present utility model includes an antibody container 1, a neutralizing solution container 2, metering pumps 3, a Y mixer 4 and a reactor 5. The liquid inlet ends of the two metering pumps 3 are respectively connected to the antibody container 1 and the neutralizing solution container 2 through hoses. The liquid outlet ends of the two metering pumps 3 are respectively connected to two of the ports of the Y mixer 4 through hoses, and the remaining port in the Y mixer 4 is connected to the reactor 5 through a hose.

[0015] The Y mixer 4 in the present utility model is equivalent to a tee connector, which enables the preliminary mixing of the antibody and the neutralizing solution.

[0016] As Figure 2 , a spiral blade 51 is provided in the inner cavity of the reactor 5, a hollow tube 52 is provided at the axis center of the inner cavity of the reactor 5, an installation hole is provided at the center of the spiral blade 51, and the installation hole is welded to the hollow tube 52. After the spiral blade 51 and the hollow tube are assembled, the outer contour surface of the spiral blade 51 fits with the inner cavity wall of the reactor 5. The purpose of the fitting is to prevent the liquid from flowing down through the gap between the spiral blade 51 and the inner cavity wall of the reactor 5, but to make the mixed liquid flow along the blade surface of the spiral blade 51.

[0017] To ensure that the liquid can be fully heated, a heating flow channel 50 is provided inside the side wall of the reactor 5, and the heating flow channel 50 is communicated with the inner cavity of the hollow tube 52. A liquid circulation port is provided on the outer wall of the reactor 5, and the mixed liquid in the reactor 5 is heated by heating a liquid (generally water). The heating flow channel is arranged between the outer wall of the reactor 5 and the inner cavity of the hollow tube 52, and heating is carried out "inside and outside" simultaneously, so that the heating is more uniform.

[0018] The reactor 5 in the present utility model has a structure with a closed upper end and an open lower end. A connecting pipe 53 is provided on the upper end surface of the reactor 5, and the connecting pipe 53 is connected to the Y mixer 4. A filter screen 54 is fixed on the open lower end surface of the reactor 5. The filter screen 54 can filter impurities.

[0019] It is possible to control the mixing ratio of the neutralizing solution and the antibody by controlling the rotation speeds of the two metering pumps, and further control the degree of neutralization. The control process is loose and flexible. When the antibody and the neutralizing solution are mixed in proportion in the Y mixer 4, the reaction is carried out in a continuous flow spiral reactor. The reaction time is controlled by the flow rate of the pump, the inner diameter of the reactor 5, the length of the spiral blade 51, etc., so as to control the neutralization effect. It is possible to heat the mixed liquid in the reactor 5 by heating a liquid to the reactor 5, so as to accelerate the achievement of the neutralization purpose.

[0020] The above are only the preferred embodiments of the present utility model. It should be noted that for those of ordinary skill in the art, several improvements can be made without departing from the principle of the present utility model, and these improvements should also be regarded as the protection scope of the present utility model.

Claims

1. An antibody neutralization mixing device, characterized in that: The invention comprises an antibody container (1), a neutralizing liquid container (2), a metering pump (3), a Y mixer (4) and a reactor (5), wherein the liquid inlet ends of the two metering pumps (3) are respectively connected to the antibody container (1) and the neutralizing liquid container (2), the liquid outlet ends of the two metering pumps (3) are connected to two ports of the Y mixer (4), the remaining port of the Y mixer (4) is connected to the reactor (5), and a spiral blade (51) is provided in the inner cavity of the reactor (5).

2. The antibody neutralization mixing device according to claim 1, characterized in that: A hollow tube (52) is provided at the axis center of the inner cavity of the reactor (5), a mounting hole is provided at the center of the spiral blade (51), the mounting hole is fixedly connected to the hollow tube (52), and a heating flow channel (50) is provided in the side of the reactor (5), the heating flow channel (50) is connected to the inner cavity of the hollow tube (52).

3. The antibody neutralization mixing device according to claim 2, characterized in that: The outer contour surface of the spiral blade (51) fits against the inner cavity wall of the reactor (5).

4. The antibody neutralization mixing device according to claim 1, characterized in that: The reactor (5) is a structure with a closed upper end and an open lower end. A connecting pipe (53) is provided on the upper end surface of the reactor (5), and the connecting pipe (53) is connected to the Y mixer (4). A filter screen (54) is fixed on the lower open end surface of the reactor (5).

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