Antibody purification device

Abstract

The utility model provides an antibody purification device, which belongs to the technical field of purification devices and comprises a centrifugal sleeve, a plurality of supports fixedly connected in the centrifugal sleeve, a plurality of first rotating shafts fixedly connected to the upper ends of the supports respectively, a plurality of first rotating arms fixedly connected to the upper ends of the first rotating shafts respectively, and a plurality of second rotating shafts fixedly connected to the lower ends of the first rotating shafts respectively. By means of the device, the problem that a traditional body purification device cannot deal with unexpected conditions is solved, for example, damage caused by accidental falling of the device causes leakage of antibodies stored in the device is solved. And the device is additionally provided with an anti-impact structure, so that the problem of leakage of antibodies stored in the device caused by accidental falling is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of purification devices, and particularly relates to an antibody purification device. Background Art

[0002] In the prior art, during the preparation of antibodies, purification operations are required before the antibodies are applied. In other words, before the antibodies are applied, it is necessary to purify and separate antibody molecules from serum or ascites containing complex protein components, and this process is antibody purification. In the process of antibody purification using the centrifugation method in the prior art, the inner liner tube adopts the existing connection method and is prone to detachment from the surface of the centrifugation sleeve. Some inner liner tubes use in-tube clamping or locking methods to achieve positioning connection with the centrifugation sleeve, resulting in low efficiency of manual locking between small-sized inner liner tubes and centrifugation sleeves.

[0003] The authorized publication number "CN 219385040 U" discloses "an antibody purification device". The utility model discloses an antibody purification device, which includes a centrifugation sleeve, an inner liner tube sleeved inside the centrifugation sleeve, and a cover body hinged to the side wall of the centrifugation sleeve. A filter membrane is arranged at the bottom of the inner liner tube. The cover body can be buckled on the centrifugation sleeve to form a sealed space inside the centrifugation sleeve. Symmetrically arranged adjusting sliding plates are provided on the side wall of the second pipe orifice at the upper section of the inner liner tube, and the outer ends of the adjusting sliding plates extend outside the pipe wall of the centrifugation sleeve. Symmetrically arranged limiting sliding grooves for guiding the rotation and sliding of the adjusting sliding plates are provided on the first pipe orifice at the upper section of the centrifugation sleeve. A limiting clamping groove is also sunken on one side of the limiting sliding groove on the second pipe orifice. The plate width of the adjusting sliding plate is smaller than the groove width of the limiting sliding groove but is equivalent to the groove width of the limiting clamping groove. The utility model can prevent the centrifugation sleeve and the inner liner tube from separating from each other during centrifugation and improve the purification efficiency of antibodies.

[0004] The above patent can solve the problem that the utility model can prevent the centrifugation sleeve and the inner liner tube from separating from each other during centrifugation and improve the purification efficiency of antibodies. However, the above patent has certain defects in use. The traditional antibody purification device does not solve the problem of coping with unexpected situations, such as damage caused by accidental falling of the device, which may lead to leakage of the antibodies stored inside the device. And this device additionally increases an anti-impact structure to prevent the problem of leakage of the antibodies stored inside the device due to accidental falling. Summary of the Utility Model

[0005] The purpose of the utility model is to provide an antibody purification device, aiming to solve the problem that the traditional antibody purification device in the prior art does not solve the problem of coping with unexpected situations, such as damage caused by accidental falling of the device, which may lead to leakage of the antibodies stored inside the device.

[0006] To achieve the above object, the utility model provides the following technical solutions:

[0007] An antibody purification device includes:

[0008] Centrifugal sleeves;

[0009] Supports, a plurality of the supports are provided, and the plurality of supports are fixedly connected inside the centrifugal sleeves;

[0010] First rotating shafts, a plurality of the first rotating shafts are provided, and the plurality of first rotating shafts are respectively fixedly connected to the upper ends of the plurality of supports;

[0011] First rotating arms, a plurality of the first rotating arms are provided, and the plurality of first rotating arms are respectively rotatably connected to the circumferential surfaces of the plurality of first rotating shafts;

[0012] Shock-proof components, a plurality of groups of the shock-proof components are provided, and the plurality of groups of shock-proof components are respectively arranged on both sides of the plurality of first rotating arms.

[0013] As a preferred solution of the utility model, each group of the shock-proof components includes a mating rotator, a second rotating shaft, a second rotating arm and a spring. The mating rotator, the second rotating shaft, the second rotating arm and the spring are all provided in plurality. The plurality of mating rotators are respectively fixedly connected to both ends of the plurality of first rotating arms, the plurality of second rotating shafts are respectively rotatably connected inside the plurality of mating rotators, the plurality of second rotating arms are respectively fixedly connected to the circumferential surfaces of the plurality of mating rotators, and the plurality of springs are respectively fixedly connected to the rear ends of the plurality of second rotating arms.

[0014] As a preferred solution of the utility model, a plurality of springs are fixedly connected inside the centrifugal sleeves.

[0015] As a preferred solution of the utility model, two buffer layers are fixedly connected inside the centrifugal sleeves.

[0016] As a preferred solution of the utility model, the front ends of the plurality of first rotating arms are all fixedly connected with inner lining tubes, and a first tube cover is slidably connected inside the inner lining tubes.

[0017] As a preferred solution of the utility model, a second tube cover is rotatably connected to the upper end of the centrifugal sleeve.

[0018] Compared with the prior art, the beneficial effects of the utility model are:

[0019] 1. In this solution, the front ends of the plurality of first rotating arms are firmly equipped with inner lining tubes, and a first tube cover is installed inside the inner lining tubes in a sliding connection manner. This structural design realizes the firm integration of the inner lining tubes and the first rotating arms, and at the same time endows the first tube cover with the ability to slide freely inside the inner lining tubes.

[0020] 2. In this solution, the use of this device solves the problem that traditional body purification devices do not address unexpected situations, such as damage caused by the accidental fall of the device, which in turn leads to the leakage of antibodies stored inside the device. This device, however, additionally incorporates an anti-impact structure to prevent the leakage of antibodies stored inside the device due to an accidental fall. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The drawings are used to provide a further understanding of the present utility model and form a part of the specification. They are used together with the embodiments of the present utility model to explain the present utility model and do not constitute a limitation to the present utility model. In the drawings:

[0022] Figure 1 is a side perspective view of the present utility model;

[0023] Figure 2 is a top perspective view of the present utility model;

[0024] Figure 3 is a first cross-sectional view of the present utility model;

[0025] Figure 4 is a second cross-sectional view of the present utility model;

[0026] In the figures: 1, centrifugal sleeve; 2, support; 3, first rotating shaft; 4, first rotating arm; 5, cooperating rotator; 6, second rotating shaft; 7, second rotating arm; 8, spring; 9, buffer layer; 10, inner lining tube; 11, first tube cap; 12, second tube cap. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the scope of protection of the present utility model.

[0028] Embodiment

[0029] Please refer to Figures 1 - 4 , the present utility model provides the following technical solutions:

[0030] An antibody purification device includes:

[0031] Centrifugal sleeve 1;

[0032] Supports 2, there are multiple supports 2, and multiple supports 2 are all fixedly connected inside the centrifugal sleeve 1;

[0033] The first rotating shaft 3, and there are multiple first rotating shafts 3, and the multiple first rotating shafts 3 are respectively fixedly connected to the upper ends of the multiple supports 2;

[0034] The first rotating arm 4, and there are multiple first rotating arms 4, and the multiple first rotating arms 4 are respectively rotatably connected to the circumferential surfaces of the multiple first rotating shafts 3;

[0035] The anti-impact assembly, and there are multiple groups of anti-impact assemblies, and the multiple groups of anti-impact assemblies are respectively arranged on both sides of the multiple first rotating arms 4.

[0036] In a specific embodiment of the present invention, a plurality of supports 2 are fixed inside the core component centrifugal sleeve 1. These supports 2 serve as the basic structure, extend upward and are respectively connected to a plurality of first rotating shafts 3. Each first rotating shaft 3 is fixedly connected to a support 2 through its upper end, and a rotatable first rotating arm 4 is installed on the circumferential surface of the rotating shaft. Such a layout enables the first rotating arm 4 to rotate synchronously around the first rotating shaft 3 when the centrifugal sleeve 1 rotates, not only effectively transmitting power but also ensuring stability during operation. In addition, to protect the system from potential mechanical impacts, multiple groups of anti-impact assemblies are specially designed. They are symmetrically arranged on both sides of each first rotating arm 4 and can absorb and disperse accidental impact forces during the rotation process, thereby improving the durability and operation efficiency of the entire device and ensuring the smoothness and safety of the antibody purification process.

[0037] Specifically, please refer to Figures 1 - 4 Each group of anti-impact assemblies includes a mating rotator 5, a second rotating shaft 6, a second rotating arm 7, and a spring 8. There are multiple mating rotators 5, second rotating shafts 6, second rotating arms 7, and springs 8. The multiple mating rotators 5 are respectively fixedly connected to both ends of the multiple first rotating arms 4. The multiple second rotating shafts 6 are respectively rotatably connected inside the multiple mating rotators 5. The multiple second rotating arms 7 are respectively fixedly connected to the circumferential surfaces of the multiple mating rotators 5. The multiple springs 8 are respectively fixedly connected to the rear ends of the multiple second rotating arms 7.

[0038] In this embodiment: The detailed structure of each anti-shock component is as follows, including a plurality of mating rotators 5, a second rotating shaft 6, a second rotating arm 7, and a spring 8. These components work together to ensure the effectiveness of the mechanical protection mechanism. Specifically, a plurality of mating rotators 5 are arranged at both ends of the first rotating arm 4 as a connecting bridge; subsequently, the second rotating shaft 6 is internally connected to these mating rotators 5 by rotation, and the second rotating arm 7 is fixed to the outer peripheral surface of the mating rotator 5 to form another level of rotating structure. The spring 8 is installed at the rear end of each second rotating arm 7, playing a role in buffering and shock absorption. This design absorbs any sudden impact force through the elastic potential energy of the spring 8, converts it into mechanical energy and then releases it, so that when the entire device faces irregular loads or accidental impacts, through the cooperation of this series of linkage components, the impact effect can be effectively alleviated, and the main structure is protected from damage.

[0039] For details, please refer to Figures 1 - 4 , and a plurality of springs 8 are fixedly connected inside the centrifugal sleeve 1.

[0040] In this embodiment: In the internal design of the centrifugal sleeve 1, a plurality of springs 8 are evenly arranged and fixedly assembled. This configuration utilizes the elastic characteristics of the springs 8 to provide dynamic support and buffering for the samples or components loaded in the sleeve during the centrifugation operation.

[0041] For details, please refer to Figures 1 - 4 , and two buffer layers 9 are fixedly connected inside the centrifugal sleeve 1.

[0042] In this embodiment: Two buffer layers 9 are fixedly installed inside the centrifugal sleeve 1. This design aims to enhance the resistance to various dynamic stresses generated during centrifugation.

[0043] For details, please refer to Figures 1 - 4 , and a lining tube 10 is fixedly connected to the front end of each of the plurality of first rotating arms 4, and a first tube cover 11 is slidably connected inside the lining tube 10.

[0044] In this embodiment: The front ends of the plurality of first rotating arms 4 are firmly equipped with a lining tube 10, and a first tube cover 11 is installed inside the lining tube 10 in a slidable connection manner. This structural design realizes the firm integration of the lining tube 10 and the first rotating arm 4, and at the same time endows the first tube cover 11 with the ability to freely slide inside the lining tube 10.

[0045] For details, please refer to Figures 1 - 4 , and a second tube cover 12 is rotatably connected to the upper end of the centrifugal sleeve 1.

[0046] In this embodiment: The top of the centrifugal sleeve 1 is equipped with a second tube cover 12 by means of a rotational connection. This design enables the second tube cover 12 to rotate and open flexibly relative to the centrifugal sleeve 1.

[0047] The working principle and usage process of the present utility model are as follows: First, load the antibody sample to be purified into the centrifuge sleeve 1. The sleeve is fixed inside by a plurality of supports 2 to enhance the structural stability. Then, the upper ends of the supports 2 are respectively connected to a plurality of first rotating shafts 3, and a rotatable first rotating arm 4 is installed on the outer periphery of the first rotating shaft 3, constituting a basic driving structure. On this basis, to reduce the possible mechanical impact during operation, each anti-impact component includes a cooperating rotator 5, a second rotating shaft 6, a second rotating arm 7, and a spring 8, which are integrated with the first rotating arm 4, and the spring 8 is used to absorb the impact energy. In addition, a multi-point spring 8 and a double-layer buffer layer 9 are added inside the centrifuge sleeve 1 to further improve the anti-vibration performance. The inner liner tube 10 assembled at the front end of the first rotating arm 4 is internally embedded with a sliding first tube cap 11 to regulate the internal flow channel. Finally, a second tube cap 12 is assembled at the top of the centrifuge sleeve 1 to achieve sealing and facilitate sample loading and unloading. By using this device, the problem that the traditional antibody purification device does not solve the problem of dealing with unexpected situations, such as breakage caused by accidental falling of the device, which in turn leads to the leakage of the antibody stored inside the device, is solved. And this device additionally increases an anti-impact structure to prevent the problem of leakage of the antibody stored inside the device due to accidental falling.

[0048] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. An antibody purification device, characterized in that include: Centrifugal cannula (1); A support (2), wherein a plurality of the supports (2) are provided, and the plurality of supports (2) are all fixedly connected to the centrifugal casing (1); A first rotating shaft (3), wherein a plurality of the first rotating shafts (3) are provided, and the plurality of the first rotating shafts (3) are respectively fixedly connected to the upper ends of the plurality of supports (2); A first rotating arm (4), wherein a plurality of the first rotating arms (4) are provided, and the plurality of the first rotating arms (4) are respectively rotatably connected to the circumferential surfaces of the plurality of first rotating shafts (3); An anti-impact component is provided, wherein the anti-impact group is provided in multiple groups, and the multiple groups of anti-impact components are respectively arranged on both sides of the multiple first rotating arms (4).

2. An antibody purification device according to claim 1, characterized in that: Each group of the anti-impact components comprises a matching rotator (5), a second rotating shaft (6), a second rotating arm (7) and a spring (8). The matching rotator (5), the second rotating shaft (6), the second rotating arm (7) and the spring (8) are provided in plurality. The matching rotators (5) are respectively fixedly connected to the two side ends of the plurality of first rotating arms (4), the second rotating shafts (6) are respectively rotatably connected in the matching rotators (5), the second rotating arms (7) are respectively fixedly connected to the circumferential surfaces of the matching rotators (5), and the springs (8) are respectively fixedly connected to the rear ends of the second rotating arms (7).

3. An antibody purification device according to claim 2, characterized in that: A plurality of springs (8) are fixedly connected inside the centrifugal casing (1).

4. An antibody purification device according to claim 3, characterized in that: Two buffer layers (9) are fixedly connected inside the centrifugal casing (1).

5. An antibody purification device according to claim 4, characterized in that: The front ends of the plurality of first rotating arms (4) are all fixedly connected to an inner liner tube (10), and a first tube cover (11) is slidably connected inside the inner liner tube (10).

6. An antibody purification device according to claim 5, characterized in that: The upper end of the centrifugal sleeve (1) is rotatably connected to a second tube cover (12).

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