Efficient bioreactor for antibody production

Abstract

The utility model discloses an efficient bioreactor for antibody production, and relates to the technical field of antibody production. The device comprises a reaction tank, three feeding funnels are fixedly connected to the top of the reaction tank, a supporting frame is fixedly connected to the position, close to the center, of the top of the reaction tank, an electric push rod is fixedly connected to the top of the supporting frame, and a push rod is fixedly connected to the output end of the bottom of the electric push rod. A fixing ring is fixedly connected to the top of the inner wall of the reaction tank, and a lifting ring is arranged in the fixing ring. The sealing block is arranged, specifically, the electric push rod is started to drive the lifting ring to move upwards through the push rod, so that the sealing block enters the discharging opening to seal the discharging opening, discharging of materials is stopped, and the materials in the feeding hopper are intermittently discharged by repeating the steps, so that the situation that the reaction efficiency is affected due to the fact that the materials are added too much at a time can be avoided; and through an intermittent mode, the efficiency can be improved, and meanwhile, the material reaction is more thorough.

Description

Technical Field

[0001] The utility model belongs to the technical field of antibody production, and particularly relates to an efficient bioreactor for antibody production. Background Technique

[0002] The bioreactor for antibody production is an important technical means. A bioreactor is a device that can simulate the internal environment of an organism and realize high-density, high-throughput, and low-cost cell culture and product production. At present, many types of bioreactors have been applied to antibody production, such as rotary bioreactors, stirred bioreactors, fixed-bed bioreactors, etc.

[0003] Existing bioreactors for antibody production usually directly put a variety of materials into a stirring tank. The reaction materials include key components such as culture medium, feed, and buffer solution, and the stirring reaction is carried out by stirring. Since the materials are added simultaneously, it will cause the problem of excessive one-time addition of materials, affecting the mixing effect, and it takes a lot of time for the stirring reaction, reducing the production efficiency. Therefore, we propose an efficient bioreactor for antibody production. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an efficient bioreactor for antibody production. By setting a sealing block, specifically starting the electric push rod to drive the lifting ring upward through the push rod, so that the sealing block enters the inside of the discharge port and seals the discharge port, then the material stops feeding. In this way, the materials in the feeding funnel are intermittently fed, which can avoid excessive one-time addition of materials affecting the reaction efficiency. The intermittent method can improve the efficiency and make the material reaction more thorough, solving the problem that existing bioreactors for antibody production usually directly put a variety of materials into the stirring tank, which will cause excessive one-time addition of materials, affecting the mixing effect, and it takes a lot of time for the stirring reaction, reducing the production efficiency.

[0005] To solve the above technical problems, the utility model is realized through the following technical solutions:

[0006] The utility model is an efficient bioreactor for antibody production, including a reaction tank. Three feeding funnels are fixedly connected to the top of the reaction tank. A support frame is fixedly connected to the top of the reaction tank near the center position. An electric push rod is fixedly connected to the top of the support frame. The bottom output end of the electric push rod is fixedly connected to a push rod. A fixed ring is fixedly connected to the inner wall top of the reaction tank. A lifting ring is arranged inside the fixed ring. A stirring assembly is arranged inside the reaction tank.

[0007] Inside the fixed ring, a lifting ring is provided. Three connecting rods are fixedly connected to the outer surface of the lifting ring. The parts connected by the three connecting rods are the same. A vertical rod is fixedly connected to the top of the connecting rod. A connecting frame is fixedly connected to the top of the vertical rod. A plurality of sealing blocks are fixedly connected to the top of the connecting frame. By providing the sealing blocks, specifically, starting the electric push rod drives the lifting ring to move upward through the push rod, so that the sealing blocks enter the inside of the discharge port to seal the discharge port, and the material stops falling. In this way, the material in the feed hopper is intermittently discharged, which can avoid excessive material added at one time and affect the reaction efficiency. The intermittent method can improve the efficiency and make the material reaction more complete.

[0008] Further, the parts connected inside the three feed hoppers are the same. A fixed disk is fixedly connected to the inner wall of the feed hopper. A conical block is fixedly connected to the center of the top of the fixed disk. A plurality of discharge ports are formed inside the fixed disk. The plurality of discharge ports are arranged in a circumferential array. The discharge ports are used to discharge the material in the feed hopper. The conical block plays a role in guiding the flow, improving the emptying rate of the feed hopper.

[0009] Further, the stirring assembly includes a fixed frame. A rotating shaft is fixedly connected to the center of the fixed frame. A plurality of fixed rods are fixedly connected to the bottom of the fixed frame. Three stirring blades are fixedly connected to the outer surfaces of the plurality of fixed rods. The top of the rotating shaft penetrates through the fixed ring and the lifting ring and extends to the outside of the reaction tank. The top and bottom of the rotating shaft are rotatably connected to the reaction tank. By providing the stirring assembly, specifically, starting the motor drives the rotating shaft to rotate. Then the rotating shaft drives the fixed frame to rotate. The fixed frame drives the stirring blades to rotate through the fixed rods. The stirring blades stir and react the material in the reaction tank. The stirring blades can increase the fluidity of the material, thereby improving the reaction efficiency and making the reaction faster.

[0010] Further, a motor is fixedly connected to the center of the bottom of the reaction tank. The bottom of the rotating shaft is fixedly connected to the output end of the top of the motor. A discharge pipe is fixedly connected to the bottom of the reaction tank. A valve is arranged on the outside of the discharge pipe. An exhaust pipe is fixedly connected to the right side of the top of the reaction tank. There are distances between the central axes of the lifting ring and the fixed ring and the rotating shaft. Connect the exhaust pipe to the air filtration equipment through a conveying pipeline. The waste gas generated by the reaction is discharged through the exhaust pipe and then enters the filtration equipment through the conveying pipeline to filter the waste gas.

[0011] Further, the connecting rod penetrates through the fixed ring and extends to the outside. The connecting rod is slidably connected to the fixed ring. The sealing block is inserted into the discharge port. The bottom of the push rod is fixedly connected to the top of the lifting ring. The push rod penetrates through the reaction tank and extends to the inside. The push rod is slidably connected to the reaction tank. When the push rod moves, it will drive the lifting ring to move upward or downward, so that the sealing block opens or seals the discharge port.

[0012] The utility model has the following beneficial effects:

[0013] By arranging a sealing block, specifically, when starting the electric push rod, the push rod drives the lifting ring to move upward, so that the sealing block enters the inside of the discharge port to seal the discharge port, and the material stops feeding. In this way, the material in the feeding funnel is intermittently fed, which can avoid excessive one-time addition of materials affecting the reaction efficiency. The intermittent method can improve the efficiency and make the material reaction more complete.

[0014] By arranging a stirring component, specifically, when starting the motor to drive the rotating shaft to rotate, the rotating shaft drives the fixing frame to rotate, and the fixing frame drives the stirring blades to rotate through the fixing rods. The stirring blades stir and react the materials in the reaction tank. The stirring blades can increase the fluidity of the materials, thereby improving the reaction efficiency and making the reaction faster.

[0015] Of course, when implementing any product of the utility model, it is not necessarily required to achieve all the above-mentioned advantages simultaneously. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0017] Figure 1 is a schematic diagram of the overall structure of the present utility model;

[0018] Figure 2 is a schematic cross-sectional view of the front of the reaction tank of the present utility model;

[0019] Figure 3 For the present utility model Figure 2 is an enlarged schematic diagram of A in;

[0020] Figure 4 is a schematic diagram of the overall structure of the fixing frame of the present utility model;

[0021] Figure 5 is a schematic diagram of the internal structure of the feeding funnel of the present utility model.

[0022] In the drawings, the list of components represented by each reference numeral is as follows:

[0023] 1. Reaction tank; 11. Feeding funnel; 111. Fixed plate; 112. Conical block; 113. Discharge port; 12. Support frame; 121. Electric push rod; 122. Push rod; 13. Fixed ring; 131. Lifting ring; 132. Connecting rod; 133. Vertical rod; 134. Connecting frame; 135. Sealing block; 14. Stirring assembly; 141. Fixed frame; 142. Rotating shaft; 143. Fixed rod; 144. Stirring blade; 2. Motor; 3. Discharge pipe; 4. Exhaust pipe. Detailed implementation manners

[0024] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0025] Please refer to Figures 1-5As shown in the figure, the utility model relates to an efficient bioreactor for antibody production, which includes a reaction tank 1. Three feeding funnels 11 are fixedly connected to the top of the reaction tank 1. A support frame 12 is fixedly connected to the position near the center of the top of the reaction tank 1. An electric push rod 121 is fixedly connected to the top of the support frame 12. A push rod 122 is fixedly connected to the output end at the bottom of the electric push rod 121. A fixed ring 13 is fixedly connected to the top inner wall of the reaction tank 1. A lifting ring 131 is arranged inside the fixed ring 13. A stirring assembly 14 is arranged inside the reaction tank 1; a lifting ring 131 is arranged inside the fixed ring 13. Three connecting rods 132 are fixedly connected to the outer surface of the lifting ring 131. The parts connected by the three connecting rods 132 are the same. A vertical rod 133 is fixedly connected to the top of the connecting rod 132. A connecting frame 134 is fixedly connected to the top of the vertical rod 133. A plurality of sealing blocks 135 are fixedly connected to the top of the connecting frame 134. By setting the sealing blocks 135, specifically, when the electric push rod 121 is started, the lifting ring 131 is driven to move upward through the push rod 122, so that the sealing blocks 135 enter the discharge port 113, and the discharge port 113 is sealed, then the material stops feeding. In this way, the material in the feeding funnel 11 is intermittently fed, which can avoid excessive one-time addition of materials and affect the reaction efficiency. The intermittent method can improve the efficiency and make the material reaction more complete. The parts connected inside the three feeding funnels 11 are the same. A fixed disk 111 is fixedly connected to the inner wall of the feeding funnel 11. A conical block 112 is fixedly connected to the center of the top of the fixed disk 111. A plurality of discharge ports 113 are opened inside the fixed disk 111. The plurality of discharge ports 113 are arranged in a circumferential array. The discharge ports 113 are used to discharge the material in the feeding funnel 11. The conical block 112 plays a role in guiding the flow, which improves the emptying rate of the feeding funnel 11. The stirring assembly 14 includes a fixed frame 141. A rotating shaft 142 is fixedly connected to the center of the fixed frame 141. A plurality of fixed rods 143 are fixedly connected to the bottom of the fixed frame 141. Three stirring blades 144 are fixedly connected to the outer surfaces of the plurality of fixed rods 143. The top of the rotating shaft 142 penetrates through the fixed ring 13 and the lifting ring 131 and extends to the outside of the reaction tank 1. The top and bottom of the rotating shaft 142 are rotatably connected to the reaction tank 1. By setting the stirring assembly 14, specifically, when the motor 2 is started to drive the rotating shaft 142 to rotate, the rotating shaft 142 drives the fixed frame 141 to rotate, and the fixed frame 141 drives the stirring blades 144 to rotate through the fixed rods 143. The stirring blades 144 stir and react the material in the reaction tank 1. The stirring blades 144 can increase the fluidity of the material, thereby improving the reaction efficiency and making the reaction faster.At the center of the bottom of the reaction tank 1, a motor 2 is fixedly connected. The bottom of the rotating shaft 142 is fixedly connected to the output end at the top of the motor 2. A discharge pipe 3 is fixedly connected to the bottom of the reaction tank 1. A valve is arranged on the outer side of the discharge pipe 3. An exhaust pipe 4 is fixedly connected to the right side at the top of the reaction tank 1. There are distances between the central axes of the lifting ring 131 and the fixed ring 13 and the rotating shaft 142. Connect the exhaust pipe 4 to the air filtration equipment through a conveying pipeline. The waste gas generated by the reaction is discharged through the exhaust pipe 4 and then enters the filtration equipment through the conveying pipeline to filter the waste gas. The connecting rod 132 passes through the fixed ring 13 and extends to the outside. The connecting rod 132 is slidably connected to the fixed ring 13. The sealing block 135 is inserted into the discharge port 113. The bottom of the push rod 122 is fixedly connected to the top of the lifting ring 131. The push rod 122 passes through the reaction tank 1 and extends to the inside. The push rod 122 is slidably connected to the reaction tank 1. When the push rod 122 moves, it will drive the lifting ring 131 to move up or down, so that the sealing block 135 opens or seals the discharge port 113.

[0026] A specific application of this embodiment is:

[0027] During use, first connect the exhaust pipe 4 to the air filtration equipment through a conveying pipeline. Then, put the materials to be reacted into the multiple feeding funnels 11 respectively. Subsequently, start the motor 2 to drive the rotating shaft 142 to rotate. Then the rotating shaft 142 drives the fixed frame 141 to rotate. The fixed frame 141 drives the stirring blades 144 to rotate through the fixing rods 143. Then start the electric push rod 121 to drive the lifting ring 131 to move downward through the push rod 122. Then the connecting rod 132 and the vertical rod 133 move downward together. The connecting frame 134 drives the sealing block 135 to move downward, thus opening the discharge port 113. Then the materials in the feeding funnel 11 are discharged into the reaction tank 1 through the discharge port 113. The conical block 112 plays a role in guiding the flow, improving the emptying rate in the feeding funnel 11. After a part of the materials are discharged, start the electric push rod 121 to drive the lifting ring 131 to move upward through the push rod 122, so that the sealing block 135 enters the discharge port 113 to seal the discharge port 113, and the materials stop feeding. In this way, the materials in the feeding funnel 11 are intermittently fed, which can avoid excessive materials added at one time affecting the reaction efficiency. By the intermittent method, the efficiency can be improved while the materials react more thoroughly. The materials entering the reaction tank 1 are stirred and reacted by the stirring blades 144. The stirring blades 144 can increase the fluidity of the materials, thereby improving the reaction efficiency and making the reaction faster. The waste gas generated by the reaction is discharged through the exhaust pipe 4 and then enters the filtration equipment through the conveying pipeline to filter the waste gas. Then, open the valve on the discharge pipe 3 for the reacted materials to be discharged.

[0028] In the description of this specification, the descriptions referring to the terms "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0029] The preferred embodiments of the present utility model disclosed above are only used to help illustrate the present utility model. The preferred embodiments do not describe all the details in detail, nor do they limit the present utility model to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification in order to better explain the principle and practical application of the present utility model, so that those skilled in the art can well understand and utilize the present utility model. The present utility model is only limited by the claims and their full scope and equivalents.

Claims

1. A high-efficiency bioreactor for antibody production, comprising a reaction tank (1), wherein three feeding funnels (11) are fixedly connected to the top of the reaction tank (1), a support frame (12) is fixedly connected to the top of the reaction tank (1) near the center, and an electric push rod (121) is fixedly connected to the top of the support frame (12), characterized in that: The bottom output end of the electric push rod (121) is fixedly connected to a push rod (122); the top of the inner wall of the reaction tank (1) is fixedly connected to a fixing ring (13); a lifting ring (131) is arranged inside the fixing ring (13); and a stirring assembly (14) is arranged inside the reaction tank (1); A lifting ring (131) is arranged inside the fixed ring (13), and three connecting rods (132) are fixedly connected to the outer surface of the lifting ring (131). The three connecting rods (132) connect the same parts. The top of the connecting rod (132) is fixedly connected to a vertical rod (133), and the top of the vertical rod (133) is fixedly connected to a connecting frame (134). The top of the connecting frame (134) is fixedly connected to a plurality of sealing blocks (135).

2. A high-efficiency bioreactor for antibody production according to claim 1, characterized in that: The three feed funnels (11) have the same internally connected parts. A fixed disk (111) is fixedly connected to the inner wall of the feed funnel (11). A conical block (112) is fixedly connected to the top center of the fixed disk (111). A plurality of discharge openings (113) are provided inside the fixed disk (111). The plurality of discharge openings (113) are arranged in a circular array.

3. A high-efficiency bioreactor for antibody production according to claim 2, characterized in that: The stirring assembly (14) comprises a fixed frame (141), a rotating shaft (142) is fixedly connected at the center of the fixed frame (141), a plurality of fixed rods (143) are fixedly connected at the bottom of the fixed frame (141), and three stirring blades (144) are fixedly connected to the outer surfaces of the plurality of fixed rods (143).

4. A high-efficiency bioreactor for antibody production according to claim 3, characterized in that: The top of the rotating shaft (142) passes through the fixing ring (13) and the lifting ring (131) and extends to the outside of the reaction tank (1), and the top and bottom of the rotating shaft (142) are both rotatably connected to the reaction tank (1).

5. A high-efficiency bioreactor for antibody production according to claim 4, characterized in that: A motor (2) is fixedly connected to the center of the bottom of the reaction tank (1), and the bottom of the rotating shaft (142) is fixedly connected to the top output end of the motor (2).

6. A high-efficiency bioreactor for antibody production according to claim 5, characterized in that: A discharge pipe (3) is fixedly connected to the bottom of the reaction tank (1), a valve is arranged on the outside of the discharge pipe (3), an exhaust pipe (4) is fixedly connected to the right side of the top of the reaction tank (1), and a spacing exists between the central axis of the lifting ring (131) and the fixed ring (13) and the rotating shaft (142).

7. A high-efficiency bioreactor for antibody production according to claim 4, characterized in that: The connecting rod (132) passes through the fixing ring (13) and extends to the outside, the connecting rod (132) is slidably connected to the fixing ring (13), the sealing block (135) is plugged into the discharge port (113), the bottom of the push rod (122) is fixedly connected to the top of the lifting ring (131), the push rod (122) passes through the reaction tank (1) and extends to the inside, and the push rod (122) is slidably connected to the reaction tank (1).

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