Organ-like embedding mold

Abstract

The utility model relates to the technical field of organoids, and discloses an organoid embedding mold which comprises two closed shells, a mold shell is mounted in each closed shell, an observation cover is placed on the top sides of the two closed shells, and an exhaust pipe is fixedly communicated with the top side of the observation cover. The rotary table rotates to drive the closed shell and the inner mold shell to rotate, so that liquid paraffin liquid is driven to rotate, and the rotary table is matched with the rotatable observation cover, so that the exhaust pipe can be kept in a static state when the rotary table rotates, air in the closed shell is exhausted, escape of gas in the paraffin liquid is accelerated, and bubbles are prevented from being generated; through the arrangement of the cavity in the mold shell and the cooperation of the connecting table and the cooling pipe, after the rotary table stops rotating, a refrigerant is injected into the mold shell, solidification of paraffin liquid is accelerated under the condition that workers are in a room-temperature environment, and the safety of the workers is guaranteed to a certain extent.

Description

Technical Field

[0001] The utility model relates to the technical field of organoids, in particular to an organoid embedding mold. Background Art

[0002] Organoids are 3D culture systems generated in vitro from pluripotent or adult stem cells by mimicking human development or organ regeneration. A major advantage of this system is the ability to expand tissue-specific stem cells and their differentiated progeny from very limited starting materials (such as biopsies), facilitating in-depth analysis of stem cell behavior, drug screening, disease modeling, and genetic screening. Organoids can mimic many aspects of natural tissues, including their structure and function, and are an important bridge between traditional 2D cultures and in vivo mouse / human models, with great potential in basic research and translational applications. Paraffin embedding is a standard technique used in clinical and laboratory research to create formalin-fixed, paraffin-embedded tissue blocks. After the tissue is processed, it is embedded in paraffin to form a paraffin block, which can be stored at room temperature for several years. The paraffin block can be cut with a microtome to form thin sections for subsequent staining.

[0003] The existing organoid embedding mold (publication number: CN219084544U) has at least the following drawbacks: when making an organoid model, it is necessary to drop the melted paraffin liquid into the mold. During this process, air bubbles are inevitably generated in the paraffin liquid, affecting the quality of the finished product. Generally, the air bubbles on the liquid surface are manually sucked out with a syringe, which is rather troublesome and the removal is not thorough; at the same time, in actual use, a low-temperature environment is required to facilitate the rapid solidification of the paraffin liquid. Therefore, it is often necessary for the staff to reach into an environment such as a refrigerator to operate, which may cause the staff to be frostbitten. Summary of the Utility Model

[0004] The purpose of the utility model is to solve the deficiencies existing in the prior art and propose an organoid embedding mold.

[0005] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0006] An organoid embedding mold includes two sealed outer shells. One side of the two sealed outer shells is hinged to each other. A mold outer shell is installed inside each of the sealed outer shells. The opposite sides of the two mold outer shells can be clamped to each other. The two sealed outer shells can rotate driven by a rotation driving source. An observation cover is placed on the top sides of the two sealed outer shells. A suction pipe is fixedly communicated with the top side of the observation cover. One end of the suction pipe is communicated with a suction gas source. A cooling pipe is slidably connected to the top side of the observation cover. One end of the cooling pipe is communicated with a cold source.

[0007] As a further solution of the present utility model, a socket is fixedly connected to each side of each mold housing. A plug board is fixedly connected to the inner wall of each sealed housing facing its hinged side. A draw board is slidably connected to the side of each sealed housing facing away from the plug board, and each draw board and plug board are adapted to the socket of the corresponding mold housing.

[0008] As a further solution of the present utility model, a cavity is provided in the interior of each mold housing, and the cavities of the two mold housings can communicate with each other. A connection platform is fixedly connected to the top side of one of the mold housings. A cooling pipe is slidably connected to the top side of the observation cover. The bottom end of the cooling pipe can extend to the inner wall of the sealed housing and can be connected to the connection platform.

[0009] As a further solution of the present utility model, a forming rod is slidably connected to the top side of the observation cover. A forming platform is fixedly connected to the top side of the forming rod. A forming hammer is fixedly connected to the bottom side of the forming rod. A spring is connected between the observation cover and the forming platform, and the spring is wound around the outside of the forming rod. The forming platform can be snap-connected to the top side of the observation cover.

[0010] As a further solution of the present utility model, a plurality of limit seats can be respectively connected to the outer wall of each sealed housing facing away from its hinged side, and the limit seats on the two sealed housings correspond to each other one by one. A rotating buckle is rotatably connected between the corresponding two limit seats.

[0011] As a further solution of the present utility model, the rotation driving source is a rotating motor. A support seat is fixedly connected to the top side of the rotating motor. An output end of the rotating motor is fixedly connected to a rotating table, and the top side of the rotating table can be in contact with the sealed housing.

[0012] Compared with the prior art, the present utility model has the following beneficial effects:

[0013] 1. Through the rotation of the rotating table, the rotation of the sealed housing and the inner mold housing therein is driven, thereby driving the rotation of the liquid paraffin. In cooperation with the rotatable observation cover, the air extraction pipe can remain stationary when the rotating table rotates, and the air in the sealed housing is extracted, thereby accelerating the escape of gas in the paraffin and avoiding the generation of bubbles.

[0014] 2. Through the arrangement of the cavity in the mold housing and the cooperation of the connection platform and the cooling pipe, after the rotating table stops rotating, the refrigerant is injected into the mold housing. In the case where the staff is in a room temperature environment, the solidification of the paraffin is accelerated, and the safety of the staff is guaranteed to a certain extent. Description of the Drawings

[0015] Figure 1Isometric view of the organoid embedding mold proposed by the present utility model;

[0016] Figure 2 Schematic structural diagram of the outer shell of the organoid embedding mold proposed by the present utility model;

[0017] Figure 3 Schematic structural diagram of the mold of the organoid embedding mold proposed by the present utility model;

[0018] Figure 4 Schematic structural diagram of the observation cover of the organoid embedding mold proposed by the present utility model;

[0019] Figure 5 Schematic structural diagram of the base of the organoid embedding mold proposed by the present utility model.

[0020] In the figure: 101, airtight outer shell; 102, draw plate; 103, handle; 104, rotating buckle; 105, insertion frame; 106, insertion plate; 107, mold outer shell; 108, connecting platform; 109, cavity; 201, observation cover; 202, forming hammer; 203, exhaust pipe; 204, cooling pipe; 205, forming table; 206, spring; 301, support seat; 302, turntable; 303, rotating motor. Detailed implementation manners

[0021] In order to make the technical means, creative features, achieved purposes and functions of the present utility model easy to understand, the present utility model will be further described below in conjunction with specific implementation manners.

[0022] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer", "front end", "rear end", "both ends", "one end", "the other end", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0023] In the description of the present utility model, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0024] Reference Figures 1 - 4 , the organoid embedding mold includes two sealed outer shells 101, one side of the two sealed outer shells 101 is hinged to each other, and a mold outer shell 107 is installed inside each sealed outer shell 101. The opposite sides of the two mold outer shells 107 can be clamped to each other. The two sealed outer shells 101 can rotate driven by a rotation driving source. A viewing cover 201 is placed on the top side of the two sealed outer shells 101. A suction pipe 203 is fixedly connected to the top side of the viewing cover 201. One end of the suction pipe 203 is connected to a suction source. A cooling pipe 204 is slidably connected to the top side of the viewing cover 201. One end of the cooling pipe 204 is connected to a cold source. In this embodiment, the rotation driving source is a rotation motor 303. A support base 301 is fixedly connected to the top side of the rotation motor 303. The output end of the rotation motor 303 is fixedly connected to a turntable 302. The top side of the turntable 302 can contact the sealed outer shell 101. Therefore, the operation of the rotation motor 303 will drive the turntable 302 to rotate, thereby driving the sealed outer shell 101 and the mold outer shell 107 to rotate, causing the air in the paraffin liquid in the mold outer shell 107 to float. At the same time, through the suction source, that is, the operation of the air pump connected to the suction pipe 203, the air in the sealed outer shell 101 is pumped away, forcing the bubbles on the surface of the paraffin oil to break, so as to ensure that there are no bubbles on the surface of the paraffin oil when it solidifies. In this embodiment, the cold source is dry ice. A cavity 109 is provided inside each mold outer shell 107, and the cavities 109 of the two mold outer shells 107 can communicate with each other. A connecting platform 108 is fixedly connected to the top side of one of the mold outer shells 107. A cooling pipe 204 is slidably connected to the top side of the viewing cover 201. The bottom end of the cooling pipe 204 can extend to the inner wall of the sealed outer shell 101, and the bottom end of the cooling pipe 204 can be connected to the connecting platform 108. The staff injects dry ice into the cavity 109 through the cooling pipe 204 and the connecting platform 108 to absorb heat and solidify the paraffin oil. At the same time, it is necessary to open holes on the surface of the mold outer shell 107 to facilitate the outflow of carbon dioxide gas.

[0025] An insertion frame 105 is fixedly connected to each side of each mold outer shell 107. A insertion plate 106 is fixedly connected to the inner wall of each sealed outer shell 101 facing its hinged side. A extraction plate 102 is slidably connected to the side of each sealed outer shell 101 opposite to the insertion plate 106, and each extraction plate 102 and the insertion plate 106 are adapted to the insertion frame 105 of the corresponding mold outer shell 107. In this embodiment, through the arrangement of the insertion plate 106 and the extraction plate 102, each sealed outer shell 101 can move synchronously with a mold outer shell 107. Therefore, when the two sealed outer shells 101 are combined, the two mold outer shells 107 also close together, and can perform its function as a mold. At the same time, the viewing cover 201 can be easily removed, thereby exposing the opening on the top side of the sealed outer shell 101, facilitating the staff to insert a dropper or forceps into the mold for operation.

[0026] A forming rod is slidably connected to the top side of the observation cover 201. A forming table 205 is fixedly connected to the top side of the forming rod. A forming hammer 202 is fixedly connected to the bottom side of the forming rod. A spring 206 is connected between the observation cover 201 and the forming table 205, and the spring 206 is wound around the outside of the forming rod. The forming table 205 can be engaged with the top side of the observation cover 201. In this embodiment, after mixing paraffin oil and organoid materials, the forming hammer 202 needs to be placed on the surface of the paraffin oil and then cooled to make the surface of the paraffin oil flat. At this time, the forming table 205 and the observation cover 201 can be engaged for shaping. The spring 206 enables the forming table 205 to generate an elastic force that separates the forming hammer 202 from the surface of the paraffin oil after being released, so that the staff can slightly pry the forming table 205 or the forming hammer 202 to separate the paraffin oil.

[0027] A plurality of limit seats can be respectively connected to the outer wall of each sealed housing 101 on the side facing away from its hinged side, and the limit seats on the two sealed housings 101 correspond to each other one by one, and a rotating buckle 104 is rotatably connected between the corresponding two limit seats. In this embodiment, a handle 103 is fixedly connected to the outside of each draw plate 102, and the limit seat is engaged with the sealed housing 101, so that one side of the rotating buckle 104 contacts the surface of the handle 103 to fix the two sealed housings 101. After the organoid is formed, the rotating buckle 104 and the limit seat are removed, the draw plate 102 is pulled out a certain length, and the two sealed housings 101 are pried open by using the handle 103, and the two mold housings 107 are also separated accordingly, so that the internally formed organoid paraffin block is demolded.

[0028] From the above description, it can be seen that the above embodiments of the present utility model achieve the following technical effects: By the rotation of the turntable 302, the sealed housing 101 and the internal mold housing 107 are driven to rotate, thereby driving the liquid paraffin liquid to rotate. Cooperating with the rotatable observation cover 201, the air in the sealed housing 101 can be pumped out while the turntable 302 is rotating, so that the gas in the paraffin liquid can escape more quickly, avoiding the generation of bubbles; Through the setting of the cavity 109 in the mold housing 107 and the cooperation of the connecting table 108 and the cooling pipe 204, after the turntable 302 stops rotating, the refrigerant is injected into the mold housing 107. When the staff is in a room temperature environment, the solidification of the paraffin liquid is accelerated, which ensures the safety of the staff to a certain extent.

[0029] The above has shown and described the basic principles, main features and advantages of the present utility model. Those skilled in the art should understand that the present utility model is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed. The scope of protection claimed by the present utility model is defined by the appended claims and their equivalents.

Claims

1. An organoid embedding mold, comprising two sealed shells (101), characterized in that: One side of the two sealed shells (101) is hinged to each other, and a mold shell (107) is installed inside each of the sealed shells (101). The opposite sides of the two mold shells (107) can be locked with each other. The two sealed shells (101) can rotate under the drive of a rotating drive source. An observation cover (201) is placed on the top side of the two sealed shells (101). The top side of the observation cover (201) is fixedly connected to an exhaust pipe (203), and one end of the exhaust pipe (203) is connected to an exhaust gas source. The top side of the observation cover (201) is slidably connected to a cooling pipe (204), and one end of the cooling pipe (204) is connected to a cold source.

2. The organoid embedding mold according to claim 1, characterized in that: Each of the two sides of the mold shell (107) is fixedly connected to an insertion frame (105), and each of the sealed shells (101) is fixedly connected to an inner wall facing its hinged side with an insertion plate (106). Each of the sealed shells (101) is slidably connected to a drawer plate (102) on the side facing away from the insertion plate (106), and each of the drawer plates (102) and the insertion plate (106) is compatible with the insertion frame (105) of the corresponding mold shell (107).

3. The organoid embedding mold according to claim 1, characterized in that: A cavity (109) is provided inside each mold shell (107), and the cavities (109) of the two mold shells (107) can be interconnected, and a connecting platform (108) is fixedly connected to the top side of one of the mold shells (107), and the bottom end of the cooling pipe (204) can extend to the inner wall of the closed shell (101), and the bottom end of the cooling pipe (204) can be connected to the connecting platform (108).

4. The organoid embedding mold according to claim 1, characterized in that: The top side of the observation cover (201) is slidably connected to a forming rod, the top side of the forming rod is fixedly connected to a forming platform (205), the bottom side of the forming rod is fixedly connected to a forming hammer (202), a spring (206) is connected between the observation cover (201) and the forming platform (205), and the spring (206) is wound around the outside of the forming rod, and the forming platform (205) can be clamped with the top side of the observation cover (201).

5. The organoid embedding mold according to claim 1, characterized in that: The outer wall of each of the sealed shells (101) on one side facing away from the hinged side thereof can be respectively connected to a plurality of limit seats, and the limit seats on the two sealed shells (101) correspond to each other one by one, and a rotating buckle (104) is rotatably connected between the two corresponding limit seats.

6. The organoid embedding mold according to claim 1, characterized in that: The rotation driving source is a rotating motor (303), the top side of the rotating motor (303) is fixedly connected to a support seat (301), the output end of the rotating motor (303) is fixedly connected to a turntable (302), and the top side of the turntable (302) can be in contact with the sealed housing (101).

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