Immunohistochemical decoloring device

Abstract

The utility model relates to the technical field of immunohistochemistry, and provides an immunohistochemical decoloring device which comprises a buffer pool, a decoloring pool and a buffer part, and the buffer pool is connected with a water source; the decoloring pool is arranged on one side, far away from a water source, of the buffer pool, a water outlet is formed in one side, far away from the buffer pool, of the decoloring pool, and the decoloring pool is used for decoloring a sample; the buffer part is arranged between the decolorizing pool and the buffer pool, and the buffer part is used for slowing down the water flow speed between the buffer pool and the decolorizing pool. According to the immunohistochemical decoloring device provided by the utility model, the buffer pool and the decoloring pool which are communicated with each other are arranged, and running water is introduced to decolor a sample, so that the cost of the whole device can be reduced, and the device is easy to produce, popularize and use; in addition, the device is small, portable and easy to carry, can move according to the requirements of the experiment process, can cover the whole experiment process, and can reduce the experiment cost and improve the experiment efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of immunohistochemistry, in particular to an immunohistochemical decolorization device. Background Art

[0002] Immunohistochemistry technology is a technology based on the principle of specific binding of antigen and antibody, which is used to locate and detect the expression of specific antigens in tissue samples. It is still a common and important basic experimental method in scientific research. Multiple staining and decolorization processes are involved in the immunohistochemistry experiment. If the operation is improper during the decolorization process, it is easy to cause all or part of the tissue to detach from the slide, affecting the aesthetics and reliability of the experimental results. Although there are already fully automatic devices in the immunohistochemistry technology that can cover the entire experimental process, the instrument equipment and supporting reagent consumables are relatively expensive and cannot be widely used. Therefore, a portable immunohistochemical decolorization device has become a technical problem to be solved urgently. Summary of the Utility Model

[0003] The utility model provides an immunohistochemical decolorization device to solve the defects of the existing immunohistochemical decolorization experimental device. By setting a buffer pool and a decolorization pool that are interconnected and introducing flowing water to decolorize the sample, the cost of the overall device can be reduced, making it easy to produce and promote; in addition, the device is small and portable, easy to carry, and can be moved according to the requirements of the experimental process. In other words, it can cover the entire experimental process, reduce the experimental cost, and improve the experimental efficiency.

[0004] The immunohistochemical decolorization device provided by the utility model includes:

[0005] A buffer pool, connected to a water source;

[0006] A decolorization pool, arranged on the side of the buffer pool away from the water source. An outlet is provided on the side of the decolorization pool away from the buffer pool, and the decolorization pool is used to decolorize the sample;

[0007] A buffer component, arranged between the decolorization pool and the buffer pool, and the buffer component is used to slow down the water flow rate between the buffer pool and the decolorization pool.

[0008] According to the immunohistochemical decolorization device provided by the utility model, the buffer component includes a partition plate, the partition plate is arranged between the decolorization pool and the buffer pool, and a first grid is provided on the partition plate, and the first grid is used to connect the buffer pool and the decolorization pool.

[0009] According to the immunohistochemical decolorization device provided by the utility model, the unit width of the first grid is a, where the value range of a is 3-7 mm.

[0010] According to the immunohistochemical decolorization device provided by the present utility model, a plurality of water passing holes are spaced on the buffer member.

[0011] According to the immunohistochemical decolorization device provided by the present utility model, it further includes a water outlet grid plate, the water outlet grid plate is arranged at the water outlet, and a second grid is arranged on the water outlet grid plate.

[0012] According to the immunohistochemical decolorization device provided by the present utility model, the unit width of the second grid is b, wherein the value range of b is 3-7 mm.

[0013] According to the immunohistochemical decolorization device provided by the present utility model, it further includes a fixing bracket, the fixing bracket is arranged in the decolorization pool, and the fixing bracket is provided with a plurality of limit grooves for placing the sample.

[0014] According to the immunohistochemical decolorization device provided by the present utility model, the fixing bracket is detachably arranged in the decolorization pool.

[0015] According to the immunohistochemical decolorization device provided by the present utility model, the fixing bracket includes one of a polytetrafluoroethylene bracket, a polyetheretherketone bracket and a polyimide bracket.

[0016] According to the immunohistochemical decolorization device provided by the present utility model, the decolorization pool includes one of a polytetrafluoroethylene decolorization pool, a polyetheretherketone decolorization pool and a polyimide decolorization pool.

[0017] For the immunohistochemical decolorization device provided by the present utility model, by providing a buffer pool and a decolorization pool that are interconnected and introducing flowing water to flush them, the water flow is continuously updated. In this way, when it is necessary to decolorize a sample, the sample is directly placed in the decolorization pool, and the decolorization treatment of the sample can be realized. In addition, the existence of the buffer pool and the buffer member can buffer the water flow from the water source and slow down its flow speed, so that the water flow slowly passes through the sample, reducing the direct scouring of the water flow on the sample and avoiding damage to the sample. Compared with the prior art, the immunohistochemical decolorization device provided by the present utility model has low operation difficulty, is simple to use, and the overall device has low cost, is easy to produce and popularize. In addition, the device is small and portable, easy to carry, and can be moved according to the requirements of the experimental process. In other words, it can cover the entire experimental process, reduce the experimental cost, and improve the experimental efficiency. Description of the Drawings

[0018] To more clearly illustrate the technical solutions in the present utility model or the prior art, the following will briefly introduce the attached drawings required for the description of the embodiments or the prior art. Obviously, the attached drawings in the following description are some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other attached drawings can also be obtained based on these drawings.

[0019] Figure 1 It is a schematic diagram of the overall structure of the immunohistochemical decolorization device provided by the embodiment of the present utility model.

[0020] Reference numerals:

[0021] 10: buffer pool; 20: decolorization pool; 21: water outlet; 22: water outlet grid plate; 30: buffer component; 31: partition board; 32: first grid; 40: fixing bracket. Detailed implementation manners

[0022] To make the objectives, technical solutions and advantages of the present utility model clearer, the following will clearly and completely describe the technical solutions in the present utility model with reference to the attached drawings in the present utility model. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present utility model.

[0023] In the description of the embodiments of the present application, it should be noted that unless otherwise clearly defined and limited, the terms "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to specific situations.

[0024] In the embodiments of the present application, unless otherwise clearly defined and limited, the first feature being "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is at a higher horizontal height than the second feature. The first feature being "under", "beneath" and "under" the second feature can be that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is at a lower horizontal height than the second feature.

[0025] In the description of this specification, the description referring to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the embodiments of this application. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0026] Figure 1 is a schematic diagram of the overall structure of the immunohistochemical decolorization device provided by the embodiments of the present utility model.

[0027] Refer to Figure 1 , the present utility model provides an immunohistochemical decolorization device. The immunohistochemical decolorization device includes a buffer pool 10, a decolorization pool 20, and a buffer component 30. The buffer pool 10 is connected to a live water source. As Figure 1 shown, a water inlet can be opened on the side wall of the buffer pool 10, and the buffer pool 10 is connected to the water source through the water inlet. In an alternative embodiment of the present utility model, the buffer pool 10 can also be placed below the water source, and any flowing water flows into the buffer pool 10 from top to bottom.

[0028] The decolorization pool 20 is provided on the side of the buffer pool 10 away from the water source. The two can be integral parts, which can avoid water seeping out from the gap between the two. Integral molding can be achieved by injection molding or casting, etc. In an alternative embodiment of the present utility model, the decolorization pool 20 and the buffer pool 10 can also be independent parts, and the two can be fixedly connected by means of clamping, bonding, or welding. In this case, a sealing groove and a sealing member can be provided at the connection between the two to enhance the sealing performance of the connection.

[0029] An outlet 21 is provided on the side of the decolorization pool 20 away from the buffer pool 10 to realize the flowing circulation of live water. The decolorization pool 20 is used for decolorizing the samples placed inside the decolorization pool 20. The buffer component 30 is provided between the decolorization pool 20 and the buffer pool 10, and the buffer component 30 is used to slow down the water flow rate between the buffer pool 10 and the decolorization pool 20. Specifically, when it is necessary to decolorize the samples, the samples can be first placed in the decolorization pool 20, and the valve of the water source is opened. The water flow will flow from the buffer pool 10 to the decolorization pool 20, and the decolorization of the samples can be realized by continuously flushing the samples with live water.

[0030] Refer to Figure 1, It can be understood that the immunohistochemical decolorization device provided by the embodiments of the present invention, by providing a buffer pool 10 and a decolorization pool 20 that are interconnected and introducing flowing water to flush them, enables the water flow to be continuously updated. In this way, when it is necessary to perform decolorization treatment on a sample, the sample can be directly placed in the decolorization pool 20 to achieve the decolorization treatment of the sample. In addition, the presence of the buffer pool 10 and the buffer component 30 can buffer the water flow from the water source and slow down its flow rate, so that the water flow can slowly flow through the sample, reducing the direct scouring of the water flow on the sample and avoiding damage to the sample. Compared with the prior art, the immunohistochemical decolorization device provided by the embodiments of the present invention has a low operation difficulty, is simple to use, and the overall cost of the device is low, making it easy to produce and promote. In addition, the device is small and portable, easy to carry, and can be moved according to the requirements of the experimental process. In other words, it can cover the entire experimental process, reduce the experimental cost, and improve the experimental efficiency.

[0031] Continue to refer to Figure 1 , In an alternative embodiment of the present invention, the buffer component 30 further includes a partition 31. The partition 31 is disposed between the decolorization pool 20 and the buffer pool 10. The partition 31 is provided with a first grid 32. The first grid 32 is used to connect the buffer pool 10 and the decolorization pool 20. It can be understood that the grid gaps on the first grid 32 allow the water flow to pass through and can slow down the flow rate of the water flow. In other words, the first grid 32 can be used to connect the buffer pool 10 and the decolorization pool 20.

[0032] It can be understood that by providing the partition 31 and setting the first grid 32 on the partition 31, on the one hand, the first grid 32 can be used to conduct the buffer pool 10 and the decolorization pool 20, and on the other hand, it can play a role in slowing down the water flow rate. In this way, during the process of decolorizing the sample, the damage to the sample caused by the water flow can be reduced, and the success rate and efficiency of the experiment can be improved.

[0033] Continue to refer to Figure 1 , In an alternative embodiment of the present invention, the first grid 32 includes a plurality of grid units, and the width of each unit is a. Among them, the value range of a is 3 to 7 mm. For example, a can be set to 3 mm, 4 mm, 5 mm, 6 mm, or 7 mm. The specific value of a can be adaptively selected according to the actual situation. It can be understood that by limiting the unit width of the first grid 32 within the above value range, the water flow rate can be controlled within a suitable range, and damage to the sample caused by excessive water flow rate can be avoided.

[0034] In an alternative embodiment of the present utility model, different from the above embodiment, the first grid 32 on the partition plate 31 can be replaced by a plurality of water passing holes. In other words, a plurality of water passing holes can be arranged at intervals on the buffer member 30. The number, size, and arrangement mode of the water passing holes can all be adaptively set according to the actual situation, and the embodiments of the present utility model will not elaborate too much here. It can be understood that the plurality of water passing holes can have an effect similar to that of the aforementioned first grid 32. That is, on the one hand, the water passing holes can be used to conduct the buffer pool 10 and the decolorization pool 20, and on the other hand, they can also play a role in slowing down the water flow velocity, so as to reduce the damage to the sample caused by the water flow during the decolorization process of the sample.

[0035] Continue to refer to Figure 1 , in an alternative embodiment of the present utility model, the immunohistochemical decolorization device further includes a first grid plate 32 for water outlet, and the first grid plate 32 for water outlet is arranged at the water outlet 21. It can be understood that the sample may move along with the water flow. By arranging the first grid plate 32 for water outlet, it can play a role in blocking the sample and avoid the sample being carried out of the decolorization pool 20 by the water flow.

[0036] In an alternative embodiment of the present utility model, similar to the foregoing embodiment, a plurality of grid units are also arranged on the water outlet grid plate 22, and the unit width of the grid is b, where the value range of b is 3 to 7 mm. For example, b can be set to 3 mm, 4 mm, 5 mm, 6 mm, or 7 mm, and the specific value of b can be adaptively selected according to the actual situation. It can be understood that by limiting the unit width of the water outlet grid plate 22 within the above value range, based on the cooperation with the aforementioned first grid 32, the water flow velocity for entering and leaving the decolorization pool 20 can be limited within a suitable range, so that the water flow velocity in the decolorization pool 20 can be kept within a suitable range, and damage to the sample caused by excessive water flow velocity can be avoided.

[0037] Continue to refer to Figure 1 , in an alternative embodiment of the present utility model, the immunohistochemical decolorization device further includes a fixing bracket 40, and the fixing bracket 40 is arranged in the decolorization pool 20. A plurality of limiting grooves are arranged on the fixing bracket 40. It can be understood that during use, the glass slide carrying the sample can be placed in the limiting grooves, and the sample can be supported and fixed by the limiting grooves, so as to avoid the sample being carried and moved by the water flow. In addition, by adjusting the direction of the fixing bracket 40, the angle between the sample and the water flow direction can also be adjusted, thereby controlling the decolorization effect of the water flow on the sample, and improving the flexibility during the experiment.

[0038] In an alternative embodiment of the present utility model, the fixing bracket 40 is detachably arranged in the decolorization tank 20. It can be understood that during the experiment of batch decolorization, such an arrangement is conducive to replacing samples at any time, which can greatly improve the efficiency of the decolorization experiment; in addition, the detachable connection between the fixing bracket 40 and the decolorization tank 20 is also conducive to the production and later maintenance of both of them.

[0039] In an alternative embodiment of the present utility model, the fixing bracket 40 includes one of a polytetrafluoroethylene bracket, a polyether ether ketone bracket, and a polyimide bracket. It can be understood that polytetrafluoroethylene, polyether ether ketone, and polyimide are all organic solvent-resistant materials. Using such materials to make the fixing bracket 40 can improve the service life of the fixing bracket 40.

[0040] In an alternative embodiment of the present utility model, the decolorization tank 20 includes one of a polytetrafluoroethylene decolorization tank, a polyether ether ketone decolorization tank, and a polyimide decolorization tank. In other words, similar to the foregoing embodiment, the decolorization tank 20 can also be made of any one of polytetrafluoroethylene, polyether ether ketone, and polyimide, which can improve the service life of the decolorization tank 20.

[0041] It should be noted that the technical solutions in the various embodiments of the present utility model can be combined with each other, but the basis for the combination is that those skilled in the art can implement it; when the combination of technical solutions results in contradictions or cannot be implemented, it should be considered that such a combination of technical solutions does not exist, that is, it does not fall within the protection scope of the present utility model.

[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present utility model, and are not intended to limit them; although the present utility model has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the various embodiments of the present utility model.

Claims

1. An immunohistochemical decolorization device, characterized in that: include: A buffer tank (10) connected to a water source; A decolorization tank (20) is arranged on a side of the buffer tank (10) away from the water source, and a water outlet (21) is arranged on the side of the decolorization tank (20) away from the buffer tank (10). The decolorization tank (20) is used to perform a decolorization process on the sample; A buffer component (30) is provided between the decolorizing tank (20) and the buffer tank (10), and the buffer component (30) is used to slow down the water flow speed between the buffer tank (10) and the decolorizing tank (20).

2. The immunohistochemical decolorization device according to claim 1, characterized in that: The buffer component (30) comprises a partition (31), the partition (31) being arranged between the decolorizing tank (20) and the buffer tank (10), the partition (31) being provided with a first grid (32), the first grid (32) being used to connect the buffer tank (10) and the decolorizing tank (20).

3. The immunohistochemical decolorization device according to claim 2, characterized in that: The unit width of the first grid (32) is a, wherein the value range of a is 3-7 mm.

4. The immunohistochemical decolorization device according to claim 1, characterized in that: The buffer component (30) is provided with a plurality of water holes at intervals.

5. The immunohistochemical decolorization device according to any one of claims 1 to 4, characterized in that: It also comprises a water outlet grid plate (22), wherein the water outlet grid plate (22) is arranged at the water outlet (21), and a second grid is arranged on the water outlet grid plate (22).

6. The immunohistochemical decolorization device according to claim 5, characterized in that: The unit width of the second grid is b, wherein the value range of b is 3-7 mm.

7. The immunohistochemical decolorization device according to any one of claims 1 to 4, characterized in that: It also comprises a fixed support (40), the fixed support (40) being arranged in the decolorization tank (20), the fixed support (40) being provided with a plurality of limiting grooves, the limiting grooves being used to place the sample.

8. The immunohistochemical decolorization device according to claim 7, characterized in that: The fixing bracket (40) is detachably arranged in the decolorizing tank (20).

9. The immunohistochemical decolorization device according to claim 7, characterized in that: The fixing bracket (40) comprises one of a polytetrafluoroethylene bracket, a polyetheretherketone bracket and a polyimide bracket.

10. The immunohistochemical decolorization device according to any one of claims 1 to 4, characterized in that: The decolorization tank (20) comprises one of a polytetrafluoroethylene decolorization tank, a polyetheretherketone decolorization tank and a polyimide decolorization tank.

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