Cell immunochemical staining box
By adopting fixed components and reduced flow components in the cell immunochemical staining box, the cell destruction problem caused by bumps and liquid injection impact during cell culture is solved, and the stability of the cell environment and the safety of liquid injection are achieved.
Patent Information
- Application Number
- CN202421738137.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-22
- Publication Date
- 2025-06-17
- Estimated Expiration
- 2034-07-22
AI Technical Summary
During cell culture, physical pressure caused by bumps can damage the cells, and the impact of liquid injection can also damage the cells.
A cellular immunochemical staining box was designed to solve the problem using fixation components and reduced flow components. The fixing assembly ensures that the test tube is stable during movement through the cooperation of the spring and the clamping plate; the flow reduction assembly adjusts the inflow rate of liquid by rotating the blocking rod and entering the water tank to avoid physical impact on the cells.
It effectively reduces the physical damage caused by cells during bumps, ensures the stability of the cellular environment, and prevents direct physical impact during liquid injection, reducing the possibility of cell damage.
Smart Images
Figure CN222994109U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of chemistry, and particularly relates to a cell immunochemistry staining box. Background Technique
[0002] Cell immunochemistry, also known as immunohistochemistry, its core concept is to use specific labeled antibodies or antigens to accurately identify and locate the corresponding antigens or antibodies in cells. Through delicate chemical color reactions, these markers can be clearly visualized under a microscope or an electron microscope, so as to realize the qualitative, localization and even quantitative detection of target substances.
[0003] During the process of cell culture, a lot of operations need to be carried out, so the cells need to be moved many times. If there is jolting during the movement, the physical impact of the jolting will cause the cell culture container to vibrate, thus causing the cells to be directly physically stressed. This kind of stress may damage the cells. For this reason, we propose a cell immunochemistry staining box. Content of the Utility Model
[0004] The purpose of the utility model is to provide a cell immunochemistry staining box to solve the problem that the pressure caused by jolting in the above-mentioned background technique may damage the cells. To achieve the above purpose, the utility model provides the following technical scheme: a cell immunochemistry staining box, including a staining box body, a fixing component is arranged on the side surface of the staining box body, the fixing component includes a rectangular groove one, the rectangular groove one is opened on the side surface of the staining box body, rectangular grooves two are opened on the inner walls of both sides of the staining box body, a spring is fixedly connected to one side of the rectangular groove two, and one end of the spring is fixedly connected to a clamping plate. By installing the fixing component, then inserting the rectangular test tube into the rectangular groove one, when the rectangular test tube touches the clamping plate and continues to be pushed inward, the two clamping plates will be squeezed to both sides until the rectangular test tube is completely inserted. Through the resilience of the spring, the two clamping plates are forced to clamp the rectangular test tube inward. When the rectangular test tube is pulled out by the pull ring, through the resilience of the spring, the two clamping plates are reset, and the rectangular test tube is firmly fixed by the clamping plates. During the movement, if there is jolting, it can also ensure that the cell environment in the test tube will not be interfered by the outside world, thus greatly reducing the possibility of cell damage.
[0005] Further preferably, a flow reduction component is arranged inside the dyeing box body. The flow reduction component includes a liquid injection port which is opened at the top of the dyeing box body and penetrates through the dyeing box body. A water blocking rod is movably connected to the inner wall of the liquid injection port by threads. A knob is fixedly connected to the bottom of the water blocking rod. By installing the flow reduction component, first, the water blocking rod is rotated and fixed by the knob. After all the rectangular test tubes are inserted, liquid is injected into the liquid injection port. The liquid first falls above the water blocking rod. When more liquid is injected, the liquid will slowly rise inside the liquid injection port. When the liquid level reaches the height of the water inlet tank, the liquid will enter the rectangular test tube through the water inlet tank. When the slide needs to be taken out, the liquid inside the rectangular test tube will flow back into the liquid injection port through the water inlet tank, preventing the direct physical impact on the cells caused by excessive water flow. Such an impact has significant potential hazards. It may not only cause the rupture of the cell membrane but also lead to the damage of cell organelles and even directly cause cell death in severe cases.
[0006] Further preferably, a storage component is arranged inside the first rectangular groove. The storage component includes a rectangular test tube which is movably connected inside the first rectangular groove. A retaining groove opening is formed inside the rectangular test tube. A second fixing rod is fixedly connected to one side of the first rectangular groove and penetrates through the first rectangular groove. A pull ring is movably connected to the side surface of the second fixing rod. A water inlet tank is formed at the bottom of the rectangular test tube and penetrates through the rectangular test tube.
[0007] Further preferably, two fixing blocks are fixedly connected to the top of the dyeing box body, and the two fixing blocks are symmetrically distributed. A first fixing rod is fixedly connected inside the two fixing blocks and penetrates through the fixing blocks. A handle is movably connected to the side surface of the first fixing rod.
[0008] Further preferably, the four first rectangular grooves are distributed in a circular array. There are four springs and two clamping plates inside each first rectangular groove.
[0009] Further preferably, the water inlet tank is communicated with the liquid injection port, and the first groove is communicated with the liquid injection port.
[0010] Compared with the prior art, the beneficial effects of the present utility model are:
[0011] In the present utility model, for this cell immunochemical staining kit, during use, by installing a fixing component, when inserting a rectangular test tube into the first rectangular groove, when the rectangular test tube touches the clamping plate and continues to be pushed inward, the two clamping plates will be squeezed to both sides until the rectangular test tube is completely inserted. Due to the resilience of the spring, the two clamping plates are forced to clamp the rectangular test tube inward. When the pull ring pulls out the rectangular test tube, due to the resilience of the spring, the two clamping plates are reset, and the rectangular test tube is firmly fixed by the clamping plates. During the movement process, if there is jolting, it can also ensure that the cell environment in the test tube will not be interfered by the outside world, thereby greatly reducing the possibility of cell damage.
[0012] In the present utility model, for this cell immunochemical staining kit, during use, by installing a flow-reducing component, first, through the action of the knob, the water-blocking rod is rotated and fixed. After all the rectangular test tubes are inserted, liquid is injected into the liquid injection port. The liquid will first fall above the water-blocking rod. Continuing to inject liquid, the liquid will slowly rise inside the liquid injection port. When the liquid level reaches the height of the water inlet tank, the liquid will enter the rectangular test tube through the water inlet tank. When it is necessary to take out the glass slide, the liquid inside the rectangular test tube will flow back into the liquid injection port through the water inlet tank, preventing the direct physical impact on the cells caused by excessive water flow. Such an impact has significant potential harmfulness, which may not only cause the rupture of the cell membrane but also lead to the damage of cell organelles, and even directly cause cell death in severe cases. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 is a three-dimensional structural schematic diagram of the present utility model;
[0014] Figure 2 is an exploded structural schematic diagram of the present utility model;
[0015] Figure 3 is of the present utility model Figure 1 magnified structural schematic diagram at A;
[0016] Figure 4 is a top three-dimensional structural schematic diagram of the present utility model;
[0017] Figure 5 is a bottom three-dimensional structural schematic diagram of the present utility model;
[0018] Figure 6 is a sectional structural schematic diagram of the present utility model.
[0019] In the figure: 1. Dyeing box body; 2. Fixing component; 3. Flow reduction component; 4. Storage component; 5. Fixed block; 6. First fixing rod; 7. Handle; 201. First rectangular groove; 202. Second rectangular groove; 203. Spring; 204. Clamping plate; 301. Liquid injection port; 302. Water blocking rod; 303. Knob; 401. Rectangular test tube; 402. Stopping groove opening; 403. Second fixing rod; 404. Pulling ring; 405. Water tank. Specific implementation manner
[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.
[0021] Please refer to Figures 1-6 , the present invention provides a technical solution: a cell immunochemical staining box, including a dyeing box body 1, a fixing component 2 is arranged on the side surface of the dyeing box body 1, the fixing component 2 includes a first rectangular groove 201, the first rectangular groove 201 is opened on the side surface of the dyeing box body 1, second rectangular grooves 202 are opened on both inner walls of the dyeing box body 1, a spring 203 is fixedly connected to one side of the second rectangular groove 202, one end of the spring 203 is fixedly connected to a clamping plate 204. By installing the fixing component 2, and then inserting the rectangular test tube 401 into the first rectangular groove 201, when the rectangular test tube 401 touches the clamping plate 204 and continues to be pushed inward, the two clamping plates 204 will be squeezed to both sides until the rectangular test tube 401 is completely inserted. By the resilience of the spring 203, the two clamping plates 204 are forced to clamp the rectangular test tube 401 inward. When the pulling ring 404 pulls out the rectangular test tube 401, by the resilience of the spring 203, the two clamping plates 204 are reset.
[0022] In this embodiment, as Figure 1 , Figure 2 and Figure 4As shown in the figure, a flow reduction component 3 is arranged inside the dyeing box body 1. The flow reduction component 3 includes a liquid injection port 301 which is opened at the top of the dyeing box body 1 and penetrates through the dyeing box body 1. The inner wall of the liquid injection port 301 is movably connected with a water blocking rod 302 through threads. The bottom of the water blocking rod 302 is fixedly connected with a knob 303. By installing the flow reduction component 3, first, under the action of the knob 303, the water blocking rod 302 is rotated and fixed. After all the rectangular test tubes 401 are inserted, liquid is injected into the liquid injection port 301. The liquid will first fall above the water blocking rod 302. When more liquid is injected, the liquid will slowly rise inside the liquid injection port 301. When the liquid level reaches the height of the water inlet tank 405, the liquid will enter the inside of the rectangular test tube 401 through the water inlet tank 405. When it is necessary to take out the glass slide, the liquid inside the rectangular test tube 401 will flow into the inside of the liquid injection port 301 through the water inlet tank 405 again.
[0023] In this embodiment, as Figure 1 , Figure 2 and Figure 3 shown, a storage component 4 is arranged inside the rectangular groove 201. The storage component 4 includes a rectangular test tube 401 which is movably connected inside the rectangular groove 201. A retaining groove 402 is opened inside the rectangular test tube 401. A second fixing rod 403 is fixedly connected to one side of the rectangular groove 201. The second fixing rod 403 penetrates through the rectangular groove 201. A pull ring 404 is movably connected to the side surface of the second fixing rod 403. A water inlet tank 405 is opened at the bottom of the rectangular test tube 401. The water inlet tank 405 penetrates through the rectangular test tube 401. By installing the storage component 4, then the glass slide is inserted into the inside of the rectangular test tube 401 through the retaining groove 402. When it is necessary to take out the rectangular test tube 401, the rectangular test tube 401 is pulled out through the pull ring 404.
[0024] In this embodiment, as Figure 1 , Figure 2 and Figure 4 shown, two fixing blocks 5 are fixedly connected to the top of the dyeing box body 1, and the two fixing blocks 5 are symmetrically distributed. A first fixing rod 6 is fixedly connected inside the two fixing blocks 5. The first fixing rod 6 penetrates through the fixing blocks 5. A handle 7 is movably connected to the side surface of the first fixing rod 6. By lifting the handle 7, the dyeing box body 1 is moved.
[0025] In this embodiment, as Figure 1 , Figure 2 and Figure 3 shown, the four rectangular grooves 201 are distributed in a circular array. There are four springs 203 inside each rectangular groove 201 and two clamping plates 204 inside each rectangular groove 201.
[0026] In this embodiment, as Figure 1 ,Figure 2 and Figure 4 As shown in Figure 4 , the water inlet tank 405 is communicated with the liquid injection port 301, and the first groove 201 is communicated with the liquid injection port 301.
[0027] The usage method and advantages of the present utility model: When using this cell immunochemical staining kit, the working process is as follows:
[0028] As Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 As shown in Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 , when in use, the user fixes the staining cassette body 1 in a suitable position, installs the storage component 4, then inserts the slide through the retaining slot 402 into the interior of the rectangular test tube 401. When it is necessary to take out the rectangular test tube 401, the rectangular test tube 401 is pulled out through the pull ring 404. By installing the fixing component 2, when the rectangular test tube 401 is inserted into the first rectangular groove 201, when the rectangular test tube 401 touches the clamping plate 204 and continues to be pushed inward, the two clamping plates 204 will be squeezed to both sides until the rectangular test tube 401 is completely inserted. Due to the resilience of the spring 203, the two clamping plates 204 are forced to clamp the rectangular test tube 401 inward. When the pull ring 404 pulls out the rectangular test tube 401, due to the resilience of the spring 203, the two clamping plates 204 are reset. By installing the flow reduction component 3, first, through the action of the knob 303, the water blocking rod 302 is rotated and fixed. After all the rectangular test tubes 401 are inserted, liquid is injected into the liquid injection port 301. The liquid will first fall above the water blocking rod 302. Continuing to inject liquid, the liquid will slowly rise inside the liquid injection port 301. When the liquid level reaches the height of the water inlet tank 405, the liquid will enter the interior of the rectangular test tube 401 through the water inlet tank 405. When it is necessary to take out the slide, the liquid inside the rectangular test tube 401 will flow through the water inlet tank 405 and into the interior of the liquid injection port 301. By lifting the handle 7, the staining cassette body 1 is moved.
[0029] The above shows and describes the basic principles, main features and advantages of the present utility model. Those skilled in the art of this industry should understand that the present utility model is not limited by the above embodiments. The above embodiments and the descriptions in the specification are only the preferred examples of the present utility model and are not used to limit the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed. The scope of protection claimed by the present utility model is defined by the appended claims and their equivalents.
Claims
1. A cell immunochemical staining box, comprising a staining box body (1), characterized in that: A fixing component (2) is provided on the side surface of the dyeing box body (1), and the fixing component (2) comprises a rectangular groove 1 (201), the rectangular groove 1 (201) is provided on the side surface of the dyeing box body (1), and rectangular grooves 2 (202) are provided on the inner walls on both sides of the dyeing box body (1), a spring (203) is fixedly connected to one side of the rectangular groove 2 (202), and a clamping plate (204) is fixedly connected to one end of the spring (203).
2. A cell immunochemical staining kit according to claim 1, characterized in that: A flow reduction component (3) is arranged inside the dyeing box body (1), and the flow reduction component (3) comprises a liquid injection port (301), the liquid injection port (301) is opened at the top of the dyeing box body (1), the liquid injection port (301) passes through the dyeing box body (1), the inner wall of the liquid injection port (301) is movably connected to a water blocking rod (302) via a thread, and the bottom of the water blocking rod (302) is fixedly connected to a knob (303).
3. A cell immunochemical staining kit according to claim 2, characterized in that: A storage component (4) is arranged inside the rectangular groove one (201), and the storage component (4) comprises a rectangular test tube (401), the rectangular test tube (401) is movably connected inside the rectangular groove one (201), a retaining notch (402) is provided inside the rectangular test tube (401), a fixing rod two (403) is fixedly connected to one side of the rectangular groove one (201), the fixing rod two (403) passes through the rectangular groove one (201), a pull ring (404) is movably connected to the side surface of the fixing rod two (403), and a water inlet groove (405) is provided at the bottom of the rectangular test tube (401), and the water inlet groove (405) passes through the rectangular test tube (401).
4. A cell immunochemical staining kit according to claim 3, characterized in that: The top of the dyeing box body (1) is fixedly connected to two fixing blocks (5), and the two fixing blocks (5) are symmetrically distributed. The insides of the two fixing blocks (5) are fixedly connected to a fixing rod 1 (6), the fixing rod 1 (6) passes through the fixing blocks (5), and the side surface of the fixing rod 1 (6) is movably connected to a handle (7).
5. A cell immunochemical staining kit according to claim 4, characterized in that: The four rectangular grooves (201) are distributed in a circular array, each of the rectangular grooves (201) has four springs (203) inside, and each of the rectangular grooves (201) has two clamping plates (204) inside.
6. A cell immunochemical staining kit according to claim 3, characterized in that: The water inlet groove (405) is connected to the liquid injection port (301), and the groove one (201) is connected to the liquid injection port (301).