Aphelenchoides besseyi inoculation culture device
By designing an inoculation and culture device for rice dry tip nematodes, the problems of inconvenience and low efficiency of existing inoculation methods are solved, and simpler and more efficient inoculation operations are achieved without affecting the growth of rice.
Patent Information
- Application Number
- CN202421733485.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-22
- Publication Date
- 2025-06-20
- Estimated Expiration
- 2034-07-22
AI Technical Summary
The existing rice dry-point nematode inoculation methods, such as leaf sheath inoculation method and seeding method, have problems such as inconvenience of operation, low inoculation efficiency and adverse effects on rice growth.
A inoculation and culture device for rice dry tip nematodes was designed, including a tube body, a bottom cover and a screen. The tube contains culture medium, the bottom cover is removable, and the screen is located between the culture medium and the bottom cover to control moisture and nematode outflow.
This device makes the inoculation operation of rice dry tip nematodes simpler and more efficient, and does not put additional burden on rice growth. By setting up a screen and bottom cover, the moisture and nematode soaking time can be effectively controlled, and the controllability of inoculation can be improved.
Smart Images
Figure CN222997231U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of nematode culture, and particularly relates to an inoculation and culture device for Aphelenchoides besseyi Background Art
[0002] The inoculation of plant parasitic nematodes is an important link in the research of nematode pathogenic mechanism in plant pathology research and teaching. Aphelenchoides besseyi is an important plant parasitic nematode that harms rice and is mainly transmitted through rice seeds. The most commonly used inoculation method for studying Aphelenchoides besseyi is the leaf sheath inoculation method. The leaf sheath inoculation method requires wrapping the leaf sheath with absorbent cotton and maintaining a high humidity after inoculation. Its disadvantages are that it is necessary to create wounds on the rice stem before inoculation, which is not conducive to the growth of rice; and the humidity on the absorbent cotton is difficult to control, resulting in low inoculation efficiency. In addition, some people also use the seed soaking method to inoculate Aphelenchoides besseyi, but the rice germination time is short, the growth of seedlings is weak, and the amount of Aphelenchoides besseyi invading is less. Therefore, developing an inoculation device with convenient operation and high inoculation efficiency is an important link in the research of the pathogenic mechanism of Aphelenchoides besseyi Summary of the Utility Model
[0003] The purpose of the utility model is to provide an inoculation and culture device for Aphelenchoides besseyi to solve the above problems and achieve the purpose of convenient inoculation operation and high inoculation efficiency of Aphelenchoides besseyi
[0004] To achieve the above purpose, the utility model provides the following scheme: An inoculation and culture device for Aphelenchoides besseyi, comprising:
[0005] A tube body, with openings respectively provided at the top and bottom of the tube body. A screen is arranged in the bottom opening of the tube body, and a culture medium is contained in the tube body;
[0006] A bottom cover, detachably connected to the bottom of the tube body, and the screen is located between the culture medium and the bottom cover.
[0007] Preferably, a plastic ring is fixedly connected in the bottom opening of the tube body, the screen is fixedly connected to the plastic ring, and the screen is arranged corresponding to the central hole of the plastic ring.
[0008] Preferably, the outer diameter of the plastic ring is 3 cm, the inner diameter is 2 cm, and the thickness is 0.5 cm.
[0009] Preferably, the diameter of the screen is 2.4 cm, and the aperture of the screen is 25 μm.
[0010] Preferably, the bottom diameter of the tube body is 3 cm, the height is 20 cm, and the thickness is 0.4 cm.
[0011] Preferably, the inner diameter of the bottom cover is 3.8 cm, and the thickness is 0.4 cm.
[0012] Preferably, the height of the culture medium contained in the tube body is half of the height of the tube body.
[0013] Compared with the prior art, the present utility model has the following advantages and technical effects:
[0014] 1. Compared with the prior art, this device has the advantages of simple structure, convenient operation, high inoculation efficiency, etc., and can be used for inoculation experiments such as the pathogenic mechanism of Aphelenchoides besseyi and the interaction mechanism with rice.
[0015] 2. By arranging a sieve mesh inside this device, it is beneficial for the excess water to flow out, but it can ensure that Aphelenchoides besseyi does not flow out with the water. The bottom cover can control the soaking time of the nematode suspension on the rice growth point at any time. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.
[0017] Figure 1 It is a schematic diagram of the inoculation device of the present utility model;
[0018] Figure 2 It is a schematic diagram of the plastic ring and sieve mesh of the present utility model;
[0019] Among them, 1, tube body; 2, sieve mesh; 3, plastic ring; 4, bottom cover; 5, culture medium. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model with reference to the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only some of the embodiments of the present utility model, rather than all of them. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present utility model.
[0021] To make the above objects, features, and advantages of the present utility model more obvious and understandable, the present utility model will be further described in detail below with reference to the drawings and specific embodiments.
[0022] Referring to Figure 1 - Figure 2 , the present utility model provides an inoculation and culture device for Aphelenchoides besseyi, including:
[0023] The tube body 1 has openings at its top and bottom respectively. A screen 2 is arranged inside the bottom opening of the tube body 1, and a culture medium 5 is contained in the tube body 1.
[0024] The bottom cover 4 is detachably connected to the bottom of the tube body 1, and the screen 2 is located between the culture medium 5 and the bottom cover 4.
[0025] The lower part of the tube body 1 mainly serves to contain the culture medium 5 to provide nutrients for the growth of rice. The upper part of the tube body 1 mainly provides space for the added suspension of rice dry tip nematodes, facilitating the inoculation of nematodes on rice. The main function of the screen 2 is to ensure that the rice dry tip nematodes in the tube body 1 do not flow out of the tube body 1 with water. The main function of the bottom cover 4 is to seal the bottom of the tube body 1. When it is necessary to drain the water in the tube body 1, this can be achieved by removing the bottom cover 4. Overall, compared with the prior art, the utility model has the advantages of simple structure, convenient operation, high inoculation efficiency, etc. It can be used for inoculation experiments such as the pathogenic mechanism of rice dry tip nematodes and the interaction mechanism with rice. At the same time, through the arranged screen, it is beneficial for the excess water to flow out, but it can ensure that the rice dry tip nematodes do not flow out of the tube with water. Through the bottom cover arranged at the bottom, the retention or outflow of the liquid in the tube body can be controlled, and the soaking time of the nematode suspension on the growth point can be controlled at any time.
[0026] In a further optimized scheme, the culture medium 5 is selected as SAP culture medium. Specifically, the culture medium 5 is configured according to the ratio of water-absorbing resin: sterile water: fine sand = 1:130:1300.
[0027] In a further optimized scheme, the tube body 1 is selected as a plastic PVC tube.
[0028] In a further optimized scheme, a plastic ring 3 is fixedly connected inside the bottom opening of the tube body 1, the screen 2 is fixedly connected to the plastic ring 3, and the screen 2 is correspondingly arranged with the central hole of the plastic ring 3.
[0029] As Figure 1 shown, the outer wall of the plastic ring 3 is adhesively bonded to the inner bottom of the tube body 1 with strong glue, and the screen 2 is pasted on the top of the plastic ring 3, so that the inner through hole of the plastic ring 3 is covered by the screen 2. At the same time, during bonding, it is ensured that the screen 2 corresponding to the central through hole of the plastic ring 3 is not covered with glue to avoid affecting the flow performance of the screen 2.
[0030] In a further optimized scheme, the outer diameter of the plastic ring 3 is 3 cm, the inner diameter is 2 cm, and the thickness is 0.5 cm.
[0031] In a further optimized scheme, the diameter of the screen 2 is 2.4 cm, and the pore diameter of the screen 2 is 25 μm.
[0032] The pore diameter of the screen 2 is selected as 25 μm, which can not only ensure that water flows out of the tube body 1, but also ensure that nematodes do not flow out of the plastic tube with water.
[0033] For a further optimized solution, the inner diameter of the bottom surface of the tube body 1 is 3 cm, the height is 20 cm, and the thickness is 0.4 cm.
[0034] For a further optimized solution, the inner diameter of the bottom cover 4 is 3.8 cm and the thickness is 0.4 cm.
[0035] For a further optimized solution, the side wall height of the bottom cover 4 is 2 cm to ensure that the bottom cover 4 is buckled on the bottom of the tube body 1 and at the same time prevent water seepage between the bottom cover 4 and the tube body 1.
[0036] The inner diameter of the bottom cover 4 is adapted to the outer diameter of the tube body 1, so that the bottom cover 4 can be directly buckled on the bottom of the tube body 1 without liquid leakage.
[0037] For a further optimized solution, the height of the culture medium 5 contained in the tube body 1 is half of the height of the tube body 1.
[0038] As Figure 1 shown, the height of the culture medium 5 is in the middle of the tube body 1, which can not only ensure sufficient growth conditions for rice seedlings, but also provide necessary space for inoculating with the suspension containing rice white tip nematodes during the inoculation and culture process.
[0039] When using the inoculation and culture device of this embodiment to inoculate rice white tip nematodes, the seed germination is combined with the original seed soaking method and leaf sheath inoculation method. The specific steps are as follows:
[0040] 1. Add the culture medium 5 to the middle position of the bottom of the tube body 1 and cover the bottom cover 4 at the bottom.
[0041] 2. Sow the disinfected rice seeds on the culture medium 5 and germinate them for 3 days under dark conditions at 28°C.
[0042] 3. After 7 days of cultivation, at the two-leaf and one-heart stage of rice seedlings, add the suspension containing 1000 rice white tip nematodes and water until the growth points of the rice seedlings are submerged.
[0043] 4. 36 hours after the rice white tip nematode suspension submerges the growth points of the rice, open the bottom cover 4 to let the excess water flow out of the tube body 1.
[0044] 5. After that, according to the conventional rice planting method, irrigate 20 mL of Hoagland nutrient solution every 3 days, and investigate the number of nematodes in the rice leaves and seeds 15 days after inoculation and at the rice filling stage respectively.
[0045] At the one-leaf-and-one-heart stage of rice, the rice white tip nematode is inoculated by soaking the growing point. The operation is simple and the inoculation efficiency can also be improved. The sieve 2 arranged in the inoculation and culture device is beneficial to the outflow of excess water, but can ensure that the rice white tip nematode does not flow out with the water. The bottom cover 4 arranged at the bottom of the pipe body 1 can control the soaking time of the nematode suspension on the growing point at any time.
[0046] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.
[0047] The embodiments described above are only for describing the preferred mode of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.
Claims
1. A rice stem nematode inoculation and cultivation device, characterized in that: include: A tube body (1), wherein the top and bottom of the tube body (1) are respectively provided with openings, a screen (2) is provided in the bottom opening of the tube body (1), and a culture medium (5) is contained in the tube body (1); A bottom cover (4) is detachably connected to the bottom of the tube body (1), and the screen (2) is located between the culture medium (5) and the bottom cover (4).
2. The inoculation and cultivation device for rice stem nematodes according to claim 1, characterized in that: A plastic ring (3) is fixedly connected to the bottom opening of the tube body (1), the screen (2) is fixedly connected to the plastic ring (3), and the screen (2) is arranged corresponding to the center hole of the plastic ring (3).
3. The inoculation and cultivation device for rice stem nematodes according to claim 2, characterized in that: The plastic ring (3) has an outer diameter of 3 cm, an inner diameter of 2 cm and a thickness of 0.5 cm.
4. The inoculation and cultivation device for rice stem nematodes according to claim 2, characterized in that: The diameter of the sieve (2) is 2.4 cm, and the pore size of the sieve (2) is 25 μm.
5. The inoculation and cultivation device for rice stem nematodes according to claim 1, characterized in that: The bottom diameter of the tube body (1) is 3 cm, the height is 20 cm and the thickness is 0.4 cm.
6. The inoculation and cultivation device for rice stem nematodes according to claim 1, characterized in that: The bottom cover (4) has an inner diameter of 3.8 cm and a thickness of 0.4 cm.
7. The inoculation and cultivation device for rice stem nematodes according to claim 1, characterized in that: The height of the culture medium (5) contained in the tube body (1) is half the height of the tube body (1).