Cholic acid chromatographic separation device
By designing a bile acid chromatography separation device with sliding rod and adjustment ring, the problems of low experimental efficiency and unsatisfactory results caused by manual operation and open environment of existing devices are solved, and more efficient separation and more accurate experimental results are achieved.
Patent Information
- Application Number
- CN202422220608.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-11
- Publication Date
- 2025-06-20
- Estimated Expiration
- 2034-09-11
AI Technical Summary
The existing bile acid chromatography separation device requires manual operation and the test tube is an open environment, which is susceptible to external interference, resulting in unsatisfactory experimental results and the debugging requires a lot of time.
A bile acid chromatography separation device is designed, including a support plate, a sliding rod, a adjustment ring and a separation tube. Through the cooperation of the sliding rod and the adjustment ring, the test tube can be flexibly adjusted and sealed, and external interference is reduced.
It improves the work efficiency and separation efficiency of the experiment, reduces the uncertainty of the experimental results, and reduces the debugging time.
Smart Images
Figure CN222998321U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of chromatography separation devices, in particular to a bile acid chromatography separation device. Background Art
[0002] Bile acids are components of bile produced in living organisms, which play important physiological roles in bile, including promoting fat digestion and absorption. A bile acid chromatography separation device is a special equipment for separating bile acids and their derivatives in a sample, which is realized through liquid chromatography technology. The main reason for using a bile acid chromatography separation device is to accurately separate and analyze bile acids and their derivatives in order to study their metabolism, physiological functions and relationships with diseases in living organisms, providing important data and information for research in fields such as medicine and biochemistry.
[0003] Existing bile acid chromatography separation devices usually include a chromatography column, packing (stationary phase), mobile phase, detector and data processing system. The chromatography column is filled with the stationary phase. When a sample passes through the column, bile acids and their derivatives move at different rates on the stationary phase and are separated according to their different interaction forces with the stationary phase. By continuously changing conditions such as the composition, flow rate and temperature of the mobile phase, the interaction between the stationary phase and the target analyte is adjusted, thereby achieving the effective separation of bile acids and their derivatives. The detector can detect the separated components.
[0004] However, the traditional bile acid chromatography separation device is manually operated by staff, and the inside of the test tube is in an open environment, making the separation experiment vulnerable to external interference, resulting in unsatisfactory experimental results. Moreover, different types of reaction test tubes are required for samples of different volumes, and debugging takes a lot of time. Therefore, a bile acid chromatography separation device is proposed. Summary of the Utility Model
[0005] To make up for the above deficiencies, the utility model provides a bile acid chromatography separation device, aiming to improve the problem that the existing device requires a long time to adjust different types of test tubes, resulting in low experimental efficiency.
[0006] To achieve the above object, the utility model provides the following technical solution: A bile acid chromatography separation device, including a support plate, the lower surface of the support plate is fixedly connected with anti-slip pads, a fixed rod is fixedly connected inside the support plate, a sliding rod is slidably connected to the outer wall of the fixed rod, a plurality of grooves two are formed inside the sliding rod, an adjusting ring is fixedly connected to the outer wall of the sliding rod, a hinge seat is fixedly connected to the outer wall of the adjusting ring, a rotating rod is rotatably connected inside the hinge seat, a spring catch is arranged inside the rotating rod, a plug is slidably connected inside the adjusting ring, the outer wall of the plug is slidably connected to the inner wall of one of the grooves two, a test tube clamp is fixedly connected inside the sliding rod, a separation tube is slidably connected inside the test tube clamp, and a fixing component is arranged inside the separation tube.
[0007] Further, the fixing component includes a guiding block, one side of the outer wall of the guiding block is fixedly connected to the inner wall of the separation tube, and an opening is formed inside the guiding block.
[0008] Further, a sealing cover is threadedly connected to the outer wall of the separation tube, and a liquid inlet pipe is fixedly connected inside the sealing cover.
[0009] Further, a funnel-shaped outlet is fixedly connected inside the liquid inlet pipe, and a sealing rubber strip is fixedly connected to the outer wall of the separation tube.
[0010] Further, a hand brake is threaded inside the sealing rubber strip, and a pressure pipeline is slidably connected to the upper surface of the support plate.
[0011] Further, a first groove is formed inside the support plate, and a clamping groove is formed inside the support plate.
[0012] Further, the outer wall of the spring catch is slidably connected to the inner wall of the clamping groove, and one side of the outer wall of the rotating rod is rotatably connected to the inner wall of the first groove.
[0013] Further, a sample pipeline is fixedly connected inside the separation tube, and a pressure pipeline is fixedly connected inside the separation tube.
[0014] The utility model has the following beneficial effects:
[0015] 1. In the utility model, by pulling the sliding rod to slide on the outer wall of the fixed rod, the slot in the adjusting ring is aligned with the groove at an appropriate position and fixed by a pin. The spring catch connected to the rotating rod is stuck in the clamping groove, which can support the sliding rod and also assist the sliding rod to make further adjustments, reducing the operation difficulty of the device and achieving the effect of improving work efficiency.
[0016] 2. In the utility model, when the reaction liquid is introduced into the separation tube through the funnel-shaped outlet and the guiding block, the reaction liquid can slide down along the inner wall of the separation tube, achieving the effect of reducing the bubbles of the reaction liquid, improving the experimental reaction rate. The sample enters the interior of the reaction liquid through the sample pipeline, avoiding the situation that the sample stays on the surface layer of the reaction liquid, and achieving the effect of improving the separation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 is a three-dimensional structure diagram of the bile acid chromatography separation device proposed by the utility model;
[0018] Figure 2 is a partial structural schematic diagram of the liquid inlet pipe of the bile acid chromatography separation device proposed by the utility model;
[0019] Figure 3 is Figure 2 an enlarged view of part A in
[0020] Legend Explanation:
[0021] 1. Support plate; 2. Anti-slip cushion block; 3. Fixed rod; 4. Sliding rod; 5. Adjusting ring; 6. Hinge seat; 7. Rotating rod; 8. Spring catch; 9. First groove; 10. Card slot; 11. Second groove; 12. Plug; 13. Test tube clamp; 14. Hand brake; 15. Sealing strip; 16. Sealing cover; 17. Funnel-shaped outlet; 18. Guide block; 19. Opening; 20. Liquid inlet pipe; 21. Pressurizing pipe; 22. Sample pipe; 23. Separation tube. Specific Embodiment
[0022] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0023] Refer to Figure 1 and Figure 2 As shown in [drawings not specified in the original text], an embodiment provided by the present invention: a cholate chromatography separation device includes a support plate 1. A non-slip cushion block 2 is fixedly connected to the lower surface of the support plate 1. A fixed rod 3 is fixedly connected inside the support plate 1. A sliding rod 4 is slidably connected to the outer wall of the fixed rod 3. A plurality of second grooves 11 are formed inside the sliding rod 4. An adjusting ring 5 is fixedly connected to the outer wall of the sliding rod 4. A hinge seat 6 is fixedly connected to the outer wall of the adjusting ring 5. A rotating rod 7 is rotatably connected inside the hinge seat 6. A spring catch 8 is arranged inside the rotating rod 7. A plug 12 is slidably connected inside the adjusting ring 5. The outer wall of the plug 12 is slidably connected to the inner wall of one of the second grooves 11. A test tube clamp 13 is fixedly connected inside the sliding rod 4. A separation tube 23 is slidably connected inside the test tube clamp 13. A fixing component is arranged inside the separation tube 23.
[0024] Specifically, a sliding rod 4 is added to the outer wall of the fixed rod 3. By pulling the adjusting ring 5, it can be aligned with the first groove 9 at an appropriate height. The plug 12 can fix the sliding rod 4 at a specific height. By pulling the adjusting ring 5, the spring catch 8 connected to the rotating rod 7 can be engaged inside different card slots 10, further adjusting the device, improving the flexibility of the separation device, and better meeting the requirements of different sample volumes.
[0025] Refer to Figure 1 、 Figure 2 and Figure 3, The fixing component includes a guiding block 18. One side of the outer wall of the guiding block 18 is fixedly connected to the inner wall of the separation tube 23. An opening 19 is provided inside the guiding block 18. A sealing cover 16 is threadedly connected to the outer wall of the separation tube 23. An inlet tube 20 is fixedly connected inside the sealing cover 16. A funnel-shaped outlet 17 is fixedly connected inside the inlet tube 20. A sealing rubber strip 15 is fixedly connected to the outer wall of the separation tube 23. A hand brake 14 is threaded inside the sealing rubber strip 15. A pressure pipeline 21 is slidably connected to the upper surface of the support plate 1. A first groove 9 is provided inside the support plate 1. A clamping groove 10 is provided inside the support plate 1. The outer wall of the spring catch 8 is slidably connected to the inner wall of the clamping groove 10. One side of the outer wall of the rotating rod 7 is rotatably connected to the inner wall of the first groove 9. A sample pipeline 22 is fixedly connected inside the separation tube 23. A pressure pipeline 21 is fixedly connected inside the separation tube 23.
[0026] Specifically, the reaction solution is introduced into the separation tube 23 through the inlet tube 20. The sealed environment inside the separation tube 23 can improve the separation efficiency, reduce the risk of sample contamination, and enhance the experimental accuracy. The reaction solution enters the separation tube 23 through the opening 19 of the guiding block 18 and flows along the wall surface, effectively reducing bubbles and improving the separation efficiency. The sample is introduced into the middle of the reaction solution through the sample pipeline 22 to prevent retention and further improve the separation efficiency. The pressure pipeline 21 is connected to a pressurizing device to increase the air pressure inside the separation tube 23 and promote the increase of the reaction rate.
[0027] Working principle: When the cholate chromatography separation device needs to be used, first, the reaction solution is introduced into the separation tube 23 through the inlet tube 20. The sealed environment inside the separation tube 23 can ensure that the separation process is not interfered by other factors and does not require manual operation, greatly improving the separation efficiency and reducing the possibility of sample contamination, and can improve the accuracy of the experiment. The introduced reaction solution can only enter the separation tube 23 through the opening 19 provided inside the guiding block 18. Such an introduction method can ensure that the reaction solution flows along the wall surface of the separation tube 23, reduce the generation of bubbles, and achieve the effect of improving the separation efficiency. Then, the sample is connected through the sample pipeline 22 to make the sample enter the middle of the reaction solution, avoiding the retention of the sample on the upper surface of the reaction solution and achieving the effect of improving the separation efficiency. The pressurizing device connected to the pressure pipeline 21 can increase the air pressure inside the separation tube 23, thereby achieving the effect of increasing the reaction rate. Due to the different volumes of the samples, many existing separation devices do not have the function of flexibly adjusting the bracket. Therefore, a sliding rod 4 is slidably connected to the outer wall of the fixing rod 3. The adjusting ring 5 can be aligned with the second groove 11 at an appropriate height by pulling the adjusting ring 5, so that the sliding rod 4 can be fixed at a specific height by the pin 12. Then, by pulling the adjusting ring 5, the spring catch 8 connected to the rotating rod 7 can be engaged inside different clamping grooves 10 for further adjustment to achieve the effect of improving the flexibility of the device.
[0028] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.
Claims
1. A bile acid chromatography separation device, comprising a support plate (1), characterized in that: The lower surface of the support plate (1) is fixedly connected with an anti-slip pad (2); the support plate (1) is fixedly connected with a fixed rod (3) inside; the fixed rod (3) is slidably connected with a sliding rod (4) on its outer wall; a plurality of grooves (11) are provided inside the sliding rod (4); an adjusting ring (5) is fixedly connected with its outer wall; a hinge seat (6) is fixedly connected with the outer wall of the adjusting ring (5); a rotating rod (7) is rotatably connected inside the hinge seat (6); a spring block (8) is provided inside the rotating rod (7); a latch (12) is slidably connected inside the adjusting ring (5); the outer wall of the latch (12) is slidably connected to the inner wall of one of the grooves (11); a test tube clamp (13) is fixedly connected inside the sliding rod (4); a separation tube (23) is slidably connected inside the test tube clamp (13); a fixing component is provided inside the separation tube (23).
2. The bile acid chromatography separation device according to claim 1, characterized in that: The fixing assembly comprises a guide block (18), one side of the outer wall of the guide block (18) is fixedly connected to the inner wall of the separation tube (23), and an opening (19) is provided inside the guide block (18).
3. The bile acid chromatography separation device according to claim 2, characterized in that: The outer wall of the separation tube (23) is threadedly connected to a sealing cover (16), and the interior of the sealing cover (16) is fixedly connected to a liquid inlet pipe (20).
4. The bile acid chromatography separation device according to claim 3, characterized in that: The liquid inlet pipe (20) is fixedly connected to a funnel-shaped outlet (17) inside, and the outer wall of the separation pipe (23) is fixedly connected to a sealing strip (15).
5. The bile acid chromatography separation device according to claim 4, characterized in that: The inner thread of the sealing rubber strip (15) is provided with a hand brake (14), and the upper surface of the support plate (1) is slidably connected with a pressurized pipe (21).
6. The bile acid chromatography separation device according to claim 1, characterized in that: A groove (9) is provided inside the support plate (1), and a clamping slot (10) is provided inside the support plate (1).
7. The bile acid chromatography separation device according to claim 6, characterized in that: The outer wall of the spring clamping block (8) is slidably connected to the inner wall of the clamping groove (10), and one side of the outer wall of the rotating rod (7) is rotatably connected to the inner wall of the groove one (9).
8. The bile acid chromatography separation device according to claim 1, characterized in that: The separation tube (23) is fixedly connected to a sample tube (22) inside, and the separation tube (23) is fixedly connected to a pressurized tube (21) inside.