Alternating electric field generation electrode plate and TTF cell culture device

Abstract

The utility model relates to an alternating electric field generating electrode plate and a TTF cell culture device, the alternating electric field generating electrode plate comprises a plate body, a power supply interface and at least one electric field generating assembly, the power supply interface comprises a first electrode and a second electrode which are opposite in polarity; the electric field generating assembly comprises a plurality of electrode rings, the electrode rings are connected to the plate body and are coaxially arranged, and the distances between every two adjacent electrode rings are equal; the electrode ring is sequentially and alternately connected with the first electrode and the second electrode from outside to inside. The TTF cell culture device comprises a culture dish, an alternating power supply and the alternating electric field generating electrode plate. The device has the advantages of wide electric field coverage range, uniform field intensity distribution, good safety, simplicity and convenience in operation, good repeatability and low experimental pollution risk.

Description

Technical Field

[0001] The utility model relates to the technical field of biological test equipment, in particular to an alternating electric field generating electrode plate and a TTF cell culture device. Background Art

[0002] In recent years, tumor treating fields (TTFields for short), as a new physical therapy technology, use medium-frequency and low-intensity alternating electric fields to effectively inhibit tumor growth while reducing the occurrence of adverse effects. TTFields are currently widely used in clinical studies of various tumor treatments, including glioblastoma, mesothelioma, non-small cell lung cancer, pancreatic cancer, ovarian cancer, liver cancer, gastric cancer, etc. This technology inhibits tumor growth by applying biophysical forces to interfere with the biological functions of tumor cells, but the specific molecular mechanism is still unclear. To study the molecular mechanism of TTFields in tumor treatment, researchers have designed and established different electrodes and alternating electric field generating platforms to study the mechanism of action of TTFields on tumor cells in vitro.

[0003] Existing TTF cell culture devices basically include the following structures: a culture dish, a circuit board, and several pairs of electrode plates. The electrode plates of the same pair are arranged oppositely, and the working voltage of these electrode plates is applied through the circuit board. When starting to cultivate cells in the culture dish, the electrode plates are placed in the culture dish to apply an alternating electric field, such as the utility model patent with the authorization announcement number CN216663117U - the TTF cell culture device. However, this approach is prone to the problem of uneven electric field intensity distribution in the culture dish, and the cells in the same culture dish receive inconsistent electric field stimulation. For example, the cells close to the electrode plates receive a greater electric field stimulation, while the cells farther away from the electrode plates receive a smaller electric field stimulation. In addition, before and after the experiment, the electrode plates need to be specially cleaned, the operation is relatively troublesome, and there is a risk of electric leakage and short circuit in the electrode plates, resulting in poor experimental safety and stability. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an alternating electric field generating electrode plate and a TTF cell culture device, which can make the electric field distribution in the culture dish uniform, and the operation is simple and convenient, with strong safety and convenient cleaning, and even can be not cleaned.

[0005] To achieve the above purpose, the utility model discloses an alternating electric field generating electrode plate, which includes a plate body, a power supply interface, and at least one electric field generating component. The power supply interface includes a first electrode and a second electrode with opposite polarities; the electric field generating component includes several electrode rings, the electrode rings are connected to the plate body and arranged coaxially, and the distances between two adjacent electrode rings are equal; the electrode rings are alternately connected to the first electrode and the second electrode in sequence from outside to inside.

[0006] With the above settings, several pairs of positive and negative electrodes of the electric field (one less than the number of electrode rings) can be formed. After connecting the power interface to the alternating power supply, an electric field with a wide coverage area and uniform field strength distribution can be formed in the area of the plate body corresponding to each electric field generating component. At this time, only need to place the culture dish on the plate body and make the culture holes on it face an electric field generating component, then stable and uniform electric field stimulation can be carried out on the cells in the culture holes. The operation is convenient and the repeatability is good. Since the electrode rings do not need to extend into the culture dish, the pollution risk of the culture dish is low, and the cleaning of the alternating electric field generating electrode plate is more convenient, and even it can be not cleaned. In addition, the liquid is not easy to contact the electrodes and electrode rings, and the safety is better.

[0007] Preferably, the width of the electrode ring is 0.1 - 1 mm, and the distance between two adjacent electrode rings is 0.5 - 2 mm. Such settings can ensure the formation and stability of the electric field, and also ensure the structural stability of the present invention.

[0008] Preferably, it further includes a first connecting member and a second connecting member; define the electrode ring connecting the first electrode as the first electrode ring, and define the electrode ring connecting the second electrode as the second electrode ring. The first electrode ring is connected to the first electrode through the first connecting member, and the second electrode ring is connected to the second electrode through the second connecting member. By providing the first connecting member and the second connecting member, it is convenient to connect the electrode ring to the power interface.

[0009] Preferably, the first connecting member, the second connecting member and the electrode ring are all located on the same surface of the plate body. The first electrode ring is provided with a first notch for avoiding the second connecting member, and the orientations of all the first notches are the same; the second electrode ring is provided with a second notch for avoiding the first connecting member, and the orientations of all the second notches are the same; the orientations of the first notch and the second notch are different. After such settings, the setting of the electric field generating component is simpler, which is beneficial to the processing and manufacturing of the present invention.

[0010] Or, the electrode ring is located on the first surface of the plate body, the first connecting member and the second connecting member are located on the second surface of the plate body, and the first surface and the second surface are in opposite directions; through holes corresponding to the electrode rings one by one are provided on the plate body, the first connecting member passes through the through hole to connect the first electrode ring, and the second connecting member passes through the through hole to connect the second electrode ring. After such settings, the first connecting member and the second connecting member will not interfere with the arrangement of the electrode rings, and a more uniform electric field can be formed.

[0011] Preferably, the outside of the electrode ring is wrapped with an insulating layer; or, the electrode ring is connected to the surface layer of the plate body, and an insulating layer is covered on the electrode ring and the surface layer of the plate body. After such settings, the use safety of the present invention can be further ensured, which is beneficial to cleaning and maintenance.

[0012] Preferably, the electric field generating components are arranged in an array on the plate body. Such an arrangement facilitates the use of the present utility model.

[0013] The present utility model also discloses a TTF cell culture device, which includes a culture dish, an alternating current power supply, and the above-mentioned alternating electric field generating electrode plate. The culture dish is placed on the plate body; the alternating current power supply is connected to the power interface. After the above arrangement, the waveform, voltage, and frequency of the electric field can be adjusted through the alternating current power supply to meet different test requirements, with strong controllability. Moreover, it also has the characteristics of convenient and simple operation, wide electric field coverage, uniform field strength distribution, and high safety. The present utility model is an external electric field generating device for in vitro cell experiments, which will not damage the closed environment where the cells in the culture dish are located, and the electrodes do not directly contact the culture medium and cells in the culture dish, with a low pollution risk.

[0014] Preferably, at least one culture dish is provided, and each culture dish is provided with at least one culture hole. The total number of the culture holes is equal to and corresponds one by one to the total number of the electric field generating components. Such an arrangement can conduct multiple groups of repetitive experiments simultaneously according to different test requirements, which is beneficial to ensuring the reliability of the experiments.

[0015] Preferably, the culture dish includes a bottom dish and a dish cover. The culture holes are arranged on the bottom dish, and the dish cover is covered on the bottom dish. Since the electrode ring does not need to extend into the culture dish, the culture dish can be relatively enclosed, so as to further reduce the experimental pollution risk and ensure the reliability of the experiment.

[0016] Preferably, a first positioning member is provided on the plate body, and a second positioning member cooperating with the first positioning member is provided on the culture dish. Such an arrangement can ensure the accurate alignment of the culture dish and the plate body, ensure the reliable progress of the experiment, and is also convenient for operation.

[0017] The present utility model has the following beneficial effects:

[0018] The present utility model can simultaneously provide at least one uniform and stable electric field, which is beneficial to the progress of repetitive experiments and has good repeatability. Moreover, the present utility model has the advantages of wide electric field coverage, uniform field strength distribution, good safety, simple operation, and low experimental pollution risk. Description of the Drawings

[0019] Figure 1 It is a schematic diagram of Example 1.

[0020] Figure 2 It is a perspective view of Example 1.

[0021] Figure 3 It is an exploded schematic diagram of Example 1.

[0022] Figure 4Schematic plan view of the alternating electric field generating electrode plate in the first embodiment.

[0023] Figure 5 Schematic diagram of the electric field generating assembly in the first embodiment.

[0024] Figure 6 Partial schematic view of the alternating electric field generating electrode plate in the second embodiment (first side of the plate body).

[0025] Figure 7 Partial schematic view of the alternating electric field generating electrode plate in the second embodiment (second side of the plate body).

[0026] Description of main component symbols:

[0027] Bottom dish 11, dish cover 12, culture well 13;

[0028] Alternating current power supply 20;

[0029] Alternating electric field generating electrode plate 30, plate body 31, power supply interface 32, first electrode 33, second electrode 34, electric field generating assembly 35, first electrode ring 36, second electrode ring 37, first connecting piece 38, second connecting piece 39. Specific implementation mode

[0030] In order to make the purpose, technical solution and advantages of the present utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments.

[0031] First embodiment

[0032] As Figures 1 to 5 shown, this embodiment discloses a TTF cell culture device, which includes a culture dish, an alternating current power supply 20 and an alternating electric field generating electrode plate 30. The alternating current power supply 20 is electrically connected to the alternating electric field generating electrode plate 30 to provide power input for it. The alternating current power supply 20 can be used to adjust the waveform, voltage and frequency. The alternating current power supply 20 is prior art and will not be elaborated here. At least one electric field generating assembly 35 for forming an alternating electric field is provided on the alternating electric field generating electrode plate 30. In this case, each electric field generating assembly 35 is the same, that is, the same alternating electric field generating electrode plate 30 generates multiple identical alternating electric fields, so that multiple groups of repetitive experiments can be carried out simultaneously.

[0033] Generally speaking, it is better to have one alternating electric field corresponding to one culture group, and the culture group is usually one culture well 13. In this case, it is described with one electric field generating component 35 corresponding to one culture well 13. In this case, six sets of electric field generating components 35 are provided on one alternating electric field generating electrode plate 30. Correspondingly, one culture dish is provided, and six culture wells 13 are provided thereon. As an alternative, two culture dishes can be provided, with three culture wells 13 provided on each culture dish; or, three culture dishes can be provided, with two culture wells 13 provided on each culture dish; or, six culture dishes can be provided, with one culture well 13 provided on each culture dish; adaptive adjustments can be made according to needs. This case is described by taking one culture dish as an example.

[0034] The culture dish includes a bottom dish 11 and a dish cover 12. The culture wells 13 are provided on the bottom dish 11 and are arranged in a 2*3 array. The dish cover 12 is then placed on the bottom dish 11, and the dish cover 12 can cover the culture wells 13 to form a relatively closed culture environment and reduce the risk of contamination.

[0035] The alternating electric field generating electrode plate 30 specifically includes a plate body 31, a power supply interface 32, a first connecting piece 38, a second connecting piece 39, and six sets of electric field generating components 35. The electric field generating components 35 are provided on the plate body 31. The culture dish is placed on the plate body 31, and the electric field generating components 35 are placed at the bottom of the culture dish and correspond to the culture wells 13 one by one, that is, the electric field generating components 35 are also arranged in a 2*3 array. A first positioning piece can be provided on the plate body 31, and a second positioning piece cooperating with the first positioning piece is provided on the culture dish to ensure accurate alignment of the culture dish and the plate body 31, ensure reliable conduct of the experiment, and facilitate operation at the same time. The first positioning piece can be a hole, and the second positioning piece is a column adapted to the hole, or the first positioning piece is a column, and the second positioning piece is a hole.

[0036] The power interface 32 includes a first electrode 33 and a second electrode 34 with opposite polarities. For example, the first electrode 33 is the positive electrode and the second electrode 34 is the negative electrode. The power interface 32 can be a socket, and the first electrode 33 and the second electrode 34 are the terminal blocks in the socket. The electric field generating component 35 includes a number of electrode rings. The electrode rings are connected to the plate body 31 and arranged coaxially. The distance between two adjacent electrode rings is equal. These electrode rings are alternately connected to the first electrode 33 and the second electrode 34 in sequence from the outside to the inside. Here, the electrode ring connected to the first electrode 33 is defined as the first electrode ring 36, and the electrode ring connected to the second electrode 34 is defined as the second electrode ring 37. Then, the arrangement of the electrode rings is that the first electrode ring 36 and the second electrode ring 37 are alternately arranged in sequence. All the first electrode rings 36 are connected to the first electrode 33 through the first connecting piece 38, and all the second electrode rings 37 are connected to the second electrode 34 through the second connecting piece 39. In this case, the first connecting piece 38, the second connecting piece 39 and all the electrode rings are on the same plane. To ensure the circuit is established, a first notch for avoiding the second connecting piece 39 is provided on the first electrode ring 36, and the orientations of all the first notches are the same; a second notch for avoiding the first connecting piece 38 is provided on the second electrode ring 37, and the orientations of all the second notches are the same; the orientations of the first notch and the second notch are different, and in this case, they are opposite to each other. After such an arrangement, the setting of the electric field generating component 35 is simpler, which is beneficial to the processing and manufacturing of the present utility model.

[0037] Through the above settings, eight pairs of electric field positive and negative poles (one less than the number of electrode rings) can be formed. After connecting the power interface 32 to the alternating power supply 20, an electric field with a wide coverage area and uniform field strength distribution can be formed in the area of the plate body 31 corresponding to each electric field generating component 35. At this time, only need to place the culture dish on the plate body 31 and make the culture holes 13 on it face an electric field generating component 35, then stable and uniform electric field stimulation can be carried out on the cells in the culture holes 13. The operation is convenient and the repeatability is good. Because the electrode rings do not need to extend into the culture dish, the pollution risk of the culture dish is low, and the cleaning of the alternating electric field generating electrode plate 30 is more convenient, and even it can be not cleaned. In addition, the liquid is not easy to contact the electrodes and the electrode rings, and the safety is better.

[0038] To ensure the formation and stability of the electric field and ensure safety, in this case, the width of the electrode ring is limited to 0.1 - 1 mm, and further limited to 0.5 mm. The distance between two adjacent electrode rings is 0.5 - 2 mm, and further limited to 1 mm. Such an arrangement can ensure that each electric field generating component 35 forms an electric field with a wide coverage area and uniform field strength distribution.

[0039] In addition, to avoid short - circuit problems, it is required that the outside of the electrode coil is wrapped with an insulating layer; or, the electrode coil is connected to the surface layer of the plate body 31, and an insulating layer is covered on the surface layers of both the electrode coil and the plate body 31. After such a setting, the use safety of the present utility model can be further ensured, which is beneficial to the cleaning and maintenance of the electrode plate.

[0040] Embodiment Two

[0041] As Figures 6 to 7 shown, the difference between this embodiment and Embodiment One is that the first electrode coil 36 and the second electrode coil 37 are arranged on the first surface (top surface) of the plate body 31, while the first connecting member 38 and the second connecting member 39 are arranged on the second surface (bottom surface) of the plate body 31, and the first surface and the second surface are in opposite directions. Through - holes corresponding to the electrode coils one by one are provided on the plate body 31. The first connecting member 38 is connected to the first electrode coil 36 by passing through the through - hole on the plate body 31, and the second connecting member 39 is connected to the second electrode coil 37 by passing through the through - hole on the plate body 31. In Figure 6 and Figure 7 the solid dots represent the connection nodes between the electrode coils and the connecting members (collectively referred to as the first connecting member 38 and the second connecting member 39). After such a setting, the electrode coil is a closed - loop circle without gaps, and the formed electric field is more uniform.

[0042] The above is only the preferred specific embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present utility model should be covered within the protection scope of the present utility model.

Claims

1. An alternating electric field generating electrode plate, characterized in that: It includes a board body, a power interface and at least one electric field generating component, wherein the power interface includes a first electrode and a second electrode with opposite polarities; the electric field generating component includes a plurality of electrode rings, which are connected to the board body and coaxially arranged, and the spacing between two adjacent electrode rings is equal; the electrode rings are alternately connected to the first electrode and the second electrode from the outside to the inside.

2. The alternating electric field generating electrode plate according to claim 1, characterized in that: The width of the electrode ring is 0.1-1 mm, and the distance between two adjacent electrode rings is 0.5-2 mm.

3. The alternating electric field generating electrode plate according to claim 1, characterized in that: It also includes a first connecting member and a second connecting member; the electrode circle connected to the first electrode is defined as the first electrode circle, and the electrode circle connected to the second electrode is defined as the second electrode circle. The first electrode circle is connected to the first electrode through the first connecting member, and the second electrode circle is connected to the second electrode through the second connecting member.

4. The alternating electric field generating electrode plate according to claim 3, characterized in that: The first connecting member, the second connecting member and the electrode ring are all located on the same surface of the plate body; the first electrode ring is provided with a first notch to avoid the second connecting member, and all the first notches are oriented in the same direction; the second electrode ring is provided with a second notch to avoid the first connecting member, and all the second notches are oriented in the same direction; the first notch and the second notch are oriented in different directions; Alternatively, the electrode ring is located on the first surface of the plate body, the first connecting member and the second connecting member are located on the second surface of the plate body, and the first surface is in opposite directions to the second surface; the plate body is provided with via holes corresponding to the electrode rings one by one, the first connecting member is connected to the first electrode ring through the via holes, and the second connecting member is connected to the second electrode ring through the via holes.

5. The alternating electric field generating electrode plate according to claim 1, characterized in that: The electrode ring is wrapped with an insulating layer outside; or, the electrode ring is connected to the surface layer of the plate body, and the surface layer of the electrode ring and the plate body is covered with an insulating layer.

6. The alternating electric field generating electrode plate according to claim 1, characterized in that: The electric field generating components are arranged in an array on the plate.

7. A TTF cell culture device, characterized in that: It comprises a culture dish, an alternating power supply and an alternating electric field generating electrode plate as claimed in any one of claims 1 to 6, wherein the culture dish is placed on the plate body; and the alternating power supply is connected to a power interface.

8. The TTF cell culture device according to claim 7, characterized in that: At least one culture dish is provided, and each culture dish is provided with at least one culture hole. The total number of the culture holes is equal to the total number of the electric field generating components and corresponds one to one.

9. The TTF cell culture device according to claim 8, characterized in that: The culture dish comprises a bottom dish and a dish cover, the culture holes are arranged on the bottom dish, and the dish cover is arranged on the bottom dish.

10. The TTF cell culture device according to claim 7, characterized in that: The plate body is provided with a first positioning piece, and the culture dish is provided with a second positioning piece matched with the first positioning piece.

Images