Tetracycline detection device

Abstract

The utility model provides a tetracycline detection device. The tetracycline detection device comprises a molecularly imprinted membrane electrochemical sensor and a portable electrochemical analysis device, the molecularly imprinted membrane electrochemical sensor comprises a screen-printed electrode and a membrane layer on the surface of a working electrode in the screen-printed electrode, wherein the membrane layer is a tetracycline molecularly imprinted polymer membrane which is synthesized by an ultrasonic method and takes graphene-gold nanoparticles as a modified material; when tetracycline is detected, the molecularly imprinted membrane electrochemical sensor is connected with a portable electrochemical analysis device. The molecularly imprinted membrane electrochemical sensor disclosed by the utility model is good in stability, and the tetracycline detection device is simple and reliable in structure, can realize rapid detection of on-site water samples, and is convenient to carry and operate.

Description

Technical Field

[0001] The utility model relates to the technical field of fiber optic jumpers, and particularly to a tetracycline detection device. Background Art

[0002] In recent years, the discharge and pollution problems of emerging pollutants have received extensive attention from society. Antibiotics are one of the main types of emerging pollutants, with characteristics such as a wide range of pollution sources, low residual concentrations, and strong environmental toxicity. Among them, tetracycline (abbreviated as TC) is one of the most widely used broad-spectrum antibiotics at present, and is often used as a feed additive and veterinary drug in the livestock and aquaculture industries. The abuse of tetracycline will cause its residues in agricultural products such as eggs and milk, and then enter the water body through biological pathways, causing harm to the environment.

[0003] At present, the commonly used detection methods for tetracycline are electrochemical analysis methods. Most of the commonly used electrochemical sensors in this method choose biological recognition elements, such as enzymes, cells, aptamers, etc. Such biosensors usually rely on the interaction between the target molecule and the biological sensitive membrane, and a biocatalytic reaction occurs through molecular recognition. The chemical signal is converted into an optical signal or an electrical signal through a transducer, and then the concentration of the target molecule is analyzed.

[0004] Due to the large difference in biological activity of biological recognition elements in different environments, biosensors are easily restricted by the detection environment. When applied to actual on-site detection, the temperature, pH, interfering substances, water impurities, etc. of the sample may all affect the stability and accuracy of the sensor. In addition, the storage time of biosensors is usually short, and they need to be stored at low temperatures. Summary of the Utility Model

[0005] In view of at least one problem in the prior art, the utility model provides a tetracycline detection device, which has good stability of the molecularly imprinted membrane electrochemical sensor, a simple and reliable structure of the tetracycline detection device, can realize the rapid detection of on-site water samples, is convenient to carry and easy to operate.

[0006] To solve the above technical problems, the utility model provides the following technical solutions:

[0007] The present application provides a tetracycline detection device, including: a molecularly imprinted membrane electrochemical sensor and a portable electrochemical analysis device;

[0008] The molecularly imprinted membrane electrochemical sensor includes: a screen-printed electrode and a film layer on the surface of the working electrode in the screen-printed electrode. The film layer is a tetracycline molecularly imprinted polymer film with graphene-gold nanoparticles synthesized by ultrasonic method as a modified material; when detecting tetracycline, the molecularly imprinted membrane electrochemical sensor is connected to the portable electrochemical analysis device.

[0009] Further, the portable electrochemical analysis device includes:

[0010] a display screen, an electrochemical sensor interface, a housing, an analysis electrode disposed within the housing, a detection data storage chip, a microprocessor, and a signal converter; the display screen is embedded in the housing, and the electrochemical sensor interface is embedded at one end of the housing;

[0011] The electrochemical sensor interface, the analysis electrode, the detection data storage chip, the microprocessor, the signal converter, and the display screen are connected in sequence.

[0012] Further, when detecting tetracycline, one end of the molecularly imprinted membrane electrochemical sensor is inserted into the solution in an electrolyte storage tank, and the other end of the molecularly imprinted membrane electrochemical sensor is connected to the electrochemical sensor interface.

[0013] Further, the tetracycline detection device further includes: a sensor storage tank and an eluent storage tank, both the sensor storage tank and the eluent storage tank are fixedly connected to the surface of the housing;

[0014] The sensor storage tank is used to store the molecularly imprinted membrane electrochemical sensor, and the eluent storage tank is used to store the eluent.

[0015] Further, a power supply is further included within the housing;

[0016] The power supply is respectively connected to the detection data storage chip, the microprocessor, the signal converter, and the display screen.

[0017] Further, the portable electrochemical analysis device further includes: a voice reminder, and the voice reminder is connected to the microprocessor;

[0018] The voice reminder is used to announce the tetracycline concentration value and / or announce the operation status of the electrochemical analysis device.

[0019] Further, a buckle is provided at one end of the side wall of the housing close to the electrochemical sensor interface;

[0020] The buckle is used to fixedly connect the portable electrochemical analysis device to an electrolyte storage tank.

[0021] Further, the solution in the electrolyte storage tank includes: a water sample and an electrolyte, the electrolyte is a 0.1 mol / L polybutylene succinate solution, and the pH value of the electrolyte is 7.4; the electrolyte is used to dilute the water sample.

[0022] Further, the eluent is a sodium hydroxide solution.

[0023] Furthermore, the housing of the portable electrochemical analysis device further includes: Bluetooth; the Bluetooth is connected to the signal converter.

[0024] As can be seen from the above technical solutions, the present invention provides a tetracycline detection device, including: a molecularly imprinted membrane electrochemical sensor and a portable electrochemical analysis device; the molecularly imprinted membrane electrochemical sensor includes: a screen-printed electrode and a film layer on the surface of the working electrode in the screen-printed electrode, and the film layer is a tetracycline molecularly imprinted polymer film with graphene-gold nanoparticles synthesized by ultrasonic method as a modification material; when detecting tetracycline, the molecularly imprinted membrane electrochemical sensor is connected to the portable electrochemical analysis device. The molecularly imprinted membrane electrochemical sensor has good stability. The tetracycline detection device has a simple and reliable structure, can realize rapid detection of on-site water samples, is convenient to carry and operate; specifically, the molecularly imprinted membrane electrochemical sensor can effectively avoid the influence of environmental factors such as temperature and pH at the detection site on the detection performance, and due to the good stability of molecular imprinting and long service life, the constructed sensor can also be stored for a long time. The portable electrochemical analysis device can be used for rapid detection of on-site water samples, and is convenient to carry and operate.

[0025] It should be understood that the above general description and the following detailed description are only exemplary and do not limit the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0027] Figure 1 It is a schematic structural diagram of the tetracycline detection device in the embodiments of the present application.

[0028] Figure 2 It is a front view schematic diagram of the molecularly imprinted membrane electrochemical sensor in the embodiments of the present application.

[0029] Figure 3 It is a cross-sectional view schematic diagram of the molecularly imprinted membrane electrochemical sensor in the embodiments of the present application.

[0030] Figure 4 It is a schematic structural diagram of the portable electrochemical analysis device in the embodiments of the present application.

[0031] Figure 5 It is a schematic diagram of the relationship between the portable electrochemical analysis device and the molecularly imprinted membrane electrochemical sensor in the embodiments of the present application.

[0032] Figure 6 This is a rear view schematic diagram of the portable electrochemical analysis device in the embodiment of the present application.

[0033] Figure 7 This is a schematic diagram showing the relationship between the sensor storage tank and the sealing cover in the embodiment of the present application.

[0034] Symbol description:

[0035] 1. Molecularly imprinted membrane electrochemical sensor; 2. Portable electrochemical analysis device; 3. Screen-printed electrode; 4. Tetracycline molecularly imprinted polymer membrane; 5. Graphene-gold nanoparticles; 6. Modified electrode; 7. Standard curve; 8. Sealing cover; 9. Housing; 10. Fixed groove; 11. Buckle; 12. Sensor storage tank; 13. Eluent storage tank; 14. Electrochemical sensor interface; 15. Analytical electrode; 16. Connecting wire; 17. Detection data storage chip; 18. Voice reminder; 19. Microprocessor; 20. Signal converter; 21. Power supply; 22. Display screen; 23. Electrolyte storage tank. Detailed implementation manners

[0036] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0037] Referring to the following description and the accompanying drawings, specific embodiments of the present utility model are disclosed in detail, indicating the ways in which the principles of the present utility model can be adopted. It should be understood that the embodiments of the present utility model are not limited thereby in scope. Within the spirit and terms of the appended claims, the embodiments of the present utility model include many changes, modifications and equivalents.

[0038] Features described and / or illustrated for one embodiment can be used in the same or similar way in one or more other embodiments, combined with the features in other embodiments, or replace the features in other embodiments.

[0039] It should be emphasized that the term "comprising / including" when used herein refers to the presence of features, whole things, steps or components, but does not exclude the presence or addition of one or more other features, whole things, steps or components.

[0040] At present, the commonly used detection methods for tetracycline also include: high performance liquid chromatography, enzyme-linked immunosorbent assay, capillary electrophoresis, etc. These methods require a long detection time, and the instruments are expensive, with complex operations and are not easily popularized. The instrument and equipment for electrochemical analysis are simple, with convenient operation, fast analysis speed, good selectivity and sensitivity, and are suitable for rapid on-site detection of trace tetracycline residues. At present, the three-electrode system is one of the most commonly used devices in the electrochemical analysis method of antibiotics. The three-electrode system consists of a working electrode, a counter electrode and a reference electrode. By adjusting the control amplifier, the potential between the working electrode and the reference electrode is made to meet the experimental requirements. Then, under the set experimental conditions, parameters such as the potential and current of the working electrode are measured to obtain a steady-state polarization curve. The use of the three-electrode system often relies on an electrochemical workstation. The electrochemical workstation can organically combine a potentiostat, a galvanostat and an electrochemical impedance analyzer, and can not only detect and store parameters such as current and voltage, but also detect the impedance parameters reflecting the battery reaction mechanism. The functions of the electrochemical workstation are usually relatively comprehensive, but due to its large volume and high price and being not easily portable, its application in on-site antibiotic concentration detection is subject to certain limitations. In addition, the common commercially available electrochemical workstations currently on the market cannot be self-powered, and a stable external power supply is required when performing analysis and detection work. The present utility model intends to adopt a three-electrode system, with an electrode modified with a molecularly imprinted polymer as the working electrode, effectively avoiding the influence of environmental factors such as temperature and pH at the detection site on the detection performance. And due to the good stability and long service life of molecular imprinting, the constructed sensor can also be stored for a long time. This device intends to combine an electrochemical detection element with a micro electrochemical workstation to propose a portable tetracycline detection device, which can be used for rapid detection of on-site water samples, and the device is convenient to carry and operate.

[0041] Specifically, it is illustrated by the following examples.

[0042] The present utility model provides an example of a tetracycline detection device. The molecularly imprinted membrane electrochemical sensor 1 has good stability. The tetracycline detection device has a simple and reliable structure, can realize rapid detection of on-site water samples, is convenient to carry and operate. Refer to Figures 1 to 3 , the tetracycline detection device includes:[[]]

[0043] A molecularly imprinted membrane electrochemical sensor 1 and a portable electrochemical analysis device 2; the molecularly imprinted membrane electrochemical sensor 1 includes: a screen-printed electrode 3 and a film layer on the surface of the working electrode in the screen-printed electrode 3, and the film layer is a tetracycline molecularly imprinted polymer film 4 with graphene-gold nanoparticles synthesized by an ultrasonic method as a modification material; when detecting tetracycline, the molecularly imprinted membrane electrochemical sensor 1 is connected to the portable electrochemical analysis device 2.

[0044] Specifically, the tetracycline molecularly imprinted polymer membrane 4 can be obtained by synthesizing graphene-gold nanoparticles as a modified material through an ultrasonic method, dropping and coating the modified material on the surface of the screen-printed electrode 3 to obtain a modified electrode, and then performing an electro-polymerization treatment on the modified electrode. The molecularly imprinted membrane electrochemical sensor 1 can be a screen-printed electrode 3 modified with a tetracycline molecularly imprinted polymer membrane 4 obtained by a dropping and coating method and an electro-polymerization method. Molecular imprinting is a process in which functional monomers and functional monomers are combined through covalent bonds or non-covalent bonds and other forces to form a specific spatial structure on the surface and inside of the polymer. After eluting the template molecules, an imprinted cavity that specifically binds to the target molecule can be formed. The recognition principle of the molecularly imprinted polymer is similar to that of antigens and antibodies, so it is also called a biomimetic recognition element. Compared with other recognition elements, the molecularly imprinted polymer has the advantages of simple preparation, good stability, good reproducibility, high selectivity, and low preparation cost.

[0045] Further, for the convenience of carrying the tetracycline detection device, as Figure 4 and Figure 5 shown, the portable electrochemical analysis device 2 may include: a display screen 22, an electrochemical sensor interface 14, a housing 9, an analysis electrode 15 disposed in the housing 9, a detection data storage chip 17, a microprocessor 19, and a signal converter 20; the display screen 22 is embedded in the housing 9, and the electrochemical sensor interface 14 is embedded at one end of the housing 9; the electrochemical sensor interface 14, the analysis electrode 15, the detection data storage chip 17, the microprocessor 19, the signal converter 20, and the display screen 22 are connected in sequence.

[0046] Specifically, the microprocessor 19 can be an MCU microprocessor, and the detection data storage chip 17 can be an existing storage chip. The housing 9 may further include: a power supply 21; the power supply 21 is respectively connected to the detection data storage chip 17, the microprocessor 19, the signal converter 20, and the display screen 22. The portable electrochemical analysis device 2 may further include: a voice reminder 18, and the voice reminder 18 is connected to the microprocessor 19; the voice reminder 18 is used to broadcast the tetracycline concentration value and / or broadcast the operation status of the electrochemical analysis device; the voice reminder 18 can be connected to the microprocessor 19 via the signal converter 20. The portable electrochemical analysis device 2 may further include: a switch, which is connected to the power supply 21 and is used to control the opening and closing of the power supply 21.

[0047] Standard samples with different concentrations can be detected by linear sweep voltammetry, and a standard curve between current and concentration is constructed and stored in the detection data storage chip 17. Turn on the power supply 21 to start the tetracycline detection. The microprocessor 19 can automatically obtain the peak current of the analysis electrode 15, and then transmit the electrical signal to the detection data storage chip 17. Through the standard curve in the detection data storage chip 17, the concentration information corresponding to the electrical signal is sorted and stored by the microprocessor 19, and the signal is transmitted to the signal converter 20. The signal converter 20 records and outputs the tetracycline concentration information, which is displayed on the display screen 22, and at the same time, the voice reminder 18 can announce the tetracycline concentration value.

[0048] Further, during the tetracycline detection, one end of the molecularly imprinted membrane electrochemical sensor 1 can be inserted into the solution in an electrolyte storage tank, and the other end of the molecularly imprinted membrane electrochemical sensor 1 is connected to the electrochemical sensor interface 14. The solution in the electrolyte storage tank may include: a water sample and an electrolyte. The electrolyte is a 0.1 mol / L polybutylene succinate solution, and the pH value of the electrolyte is 7.4; the electrolyte is used to dilute the water sample.

[0049] Further, for the convenience of carrying and eluting the molecularly imprinted membrane electrochemical sensor 1, as Figure 6 shown, the tetracycline detection device further includes: a sensor storage tank 12 and an eluent storage tank 13; both the sensor storage tank and the eluent storage tank are fixedly connected to the surface of the housing 9. Both the sensor storage tank and the eluent storage tank are detachably fixedly connected to the surface of the housing 9; the eluent can be a sodium hydroxide solution. On one side of the housing 9 opposite to the side where the display screen 22 is located, a sensor storage tank 12 and an eluent storage tank 13 can be provided. The surface of the housing may also be provided with a fixing groove 10 for clamping the sensor storage tank and the eluent storage tank on the housing. The fixing groove can be U-shaped or square rubber material, and by squeezing the side of the storage tank, the storage tank is fixed on the housing. As Figure 7 shown, the sensor storage tank 12 can be provided with a sealing cover 8; the eluent storage tank 13 can also be provided with a sealing cover, and the structure can be the same as that of the sensor storage tank and its sealing cover.

[0050] Specifically, after the test, the molecularly imprinted membrane electrochemical sensor 1 is taken out from the electrolyte storage tank, washed and then put into the eluent storage tank 13 for elution for 10 minutes, and then washed with ultrapure water and dried, and put into the sensor storage tank 12 for convenient use in the next detection.

[0051] Further, in order to improve the stability of the molecularly imprinted membrane electrochemical sensor 1 during tetracycline detection and avoid the influence of the shaking of the molecularly imprinted membrane electrochemical sensor 1 on the test results, one end of the side wall of the housing 9 close to the electrochemical sensor interface 14 is fixedly connected to an electrolyte storage tank via a buckle 11.

[0052] Further, in order to facilitate the collection of the tetracycline concentration, the housing 9 of the portable electrochemical analysis device 2 may further include: Bluetooth; the Bluetooth is connected to the signal converter. The Bluetooth may be connected to the signal converter via a microprocessor. The Bluetooth may be wirelessly communicatively connected to a remote terminal device, and may send the tetracycline concentration to the remote terminal device, facilitating the collection and subsequent processing of the tetracycline concentration. The remote terminal device may be a mobile phone or a tablet computer, etc.

[0053] Based on the above, the present application also provides an application example of a tetracycline detection device. In this application example, the device includes: a molecularly imprinted membrane electrochemical sensor 1 and a portable electrochemical analysis device 2; the molecularly imprinted membrane electrochemical sensor 1 includes: a screen-printed electrode 3 and a tetracycline molecularly imprinted polymer membrane 4; the tetracycline molecularly imprinted polymer membrane 4 is obtained by drop-coating graphene-gold nanoparticles 5 synthesized by ultrasonic method as a modification material on the surface of the screen-printed electrode 3 to obtain a modified electrode 6. The purpose of electrode modification is to improve the conductivity of the electrode and provide a large specific surface area; the molecularly imprinted membrane electrochemical sensor 1 is prepared by electropolymerization on the modified electrode 6. The specific steps are as follows: Immerse the modified electrode 6 in a phosphate buffer solution with a pH of 7.4 containing pyrrole and tetracycline, perform cyclic voltammetry scanning, and then use NaOH solution as the eluent to perform cyclic voltammetry scanning within a certain potential range to obtain the molecularly imprinted membrane electrochemical sensor 1; Take the molecularly imprinted membrane electrochemical sensor 1 as the working electrode, adopt a three-electrode system, enrich for a period of time in a phosphate buffer solution with a pH of 7.4 containing different concentrations of tetracycline, and then detect by linear sweep voltammetry to obtain a standard curve 7 between the solution concentration and the current signal; The portable electrochemical analysis device 2 uses a rectangular housing 9; A fixed groove 10, a buckle 11, a sensor storage groove 12, and an eluent storage groove 13 are installed on the surface of the housing 9; The housing 9 contains an electrochemical sensor interface 14, an analysis electrode 15, a connecting wire 16, a detection data storage chip 17, a voice reminder 18, a microprocessor 19, a signal converter 20, a power supply 21, and a display screen 22; The standard curve 7 needs to be input into the detection data storage chip 17. The detection data storage chip 17 is electrically connected to the analysis electrode 15 through the connecting wire 16; The molecularly imprinted membrane electrochemical sensor 1 needs to be connected to the electrochemical sensor interface 14 to dock the molecularly imprinted membrane electrochemical sensor 1 with the analysis electrode 15; Before using the portable electrochemical analysis device 2, it is necessary to first open the sensor storage groove 12, take out the molecularly imprinted membrane electrochemical sensor 1, then add electrolyte to an external electrolyte storage groove 23, and connect it to the housing 9 through the buckle 11; During the detection process of the portable electrochemical analysis device 2, the microprocessor 19 will obtain the peak current of the working electrode, combine it with the standard curve 7, convert the electrical signal into the concentration of tetracycline, and then display the concentration information on the display screen 22 through the signal converter 20; The display screen 22, the signal converter 20, the detection data storage chip 17, the voice reminder 18, and the microprocessor 19 are electrically connected to the power supply 21.

[0054] When detecting tetracycline, first, prepare a screen-printed electrode 3 modified with a tetracycline molecularly imprinted polymer membrane 4 by drop-coating method and electropolymerization method, and then detect standard samples with different concentrations by linear sweep voltammetry to construct a standard curve 7 between current and concentration.

[0055] Secondly, prepare before detection. First, turn on the power supply 21, confirm that the display screen 22 is operating normally. Meanwhile, control the diagnosis of the electrode situation through the microprocessor 19, and broadcast through the voice reminder 18 that all devices are operating normally, then turn off the power supply. Take a water sample on-site, dilute it 5 times with 0.1 mol / L PBS (pH 7.4) solution. After immersing the molecularly imprinted membrane electrochemical sensor 1 into the dilution solution, enrich for 10 min. After taking out the molecularly imprinted membrane electrochemical sensor 1, wash it with ultrapure water and dry it. Connect the molecularly imprinted membrane electrochemical sensor 1 to the electrochemical sensor interface 14 of the portable electrochemical analysis device 2, ensure that its connection with the analysis electrode 15 is unobstructed. Then add 5 mL of 0.1 mol / L PBS (pH 7.4) solution as the electrolyte into the external electrolyte storage tank 23, and fix the electrolyte storage tank 23 to the housing 9 with the buckle 11.

[0056] Thirdly, start sample detection. Turn on the power supply 21, the microprocessor 19 automatically obtains the peak current of the working analysis electrode, and then transmits the electrical signal to the detection data storage chip 17. Through the standard curve 7 in the detection data storage chip 17, the concentration information corresponding to the electrical signal is sorted and stored by the microprocessor 19, and the signal is transmitted into the signal converter 20. The signal converter 20 records and outputs the tetracycline concentration information, which is displayed on the display screen 22, and meanwhile the voice reminder 18 broadcasts the tetracycline concentration value.

[0057] Finally, after the test is completed, take out the molecularly imprinted membrane electrochemical sensor 1 from the electrolyte storage tank 23, wash it and put it into the eluent storage tank 13 for elution for 10 min, then wash it with ultrapure water and dry it, and put it into the sensor storage tank 12 for convenient use in the next detection.

[0058] For the tetracycline detection device provided by the present utility model, the modified electrode can be prepared by the drop coating method and the electro-polymerization method. The preparation method is simple, the preparation cost is low, and the constructed molecularly imprinted polymer has good selectivity and can specifically recognize the target molecule. The constructed portable electrochemical analysis device only needs to put the electrical sensor and the electrolyte during operation, and then can start detection after turning on the switch. The operation is simple, the device is small and inexpensive, and it is suitable for on-site sample detection. The detection method based on the molecularly imprinted electrochemical sensor has a lower detection limit, good reproducibility and stability, and the lowest detection limit of the method is far lower than the specified pollutant emission concentration.

[0059] In this utility model, specific embodiments are used to elaborate on the principles and implementation manners of the utility model. The description of the above embodiments is only used to help understand the method and its core idea of the utility model; at the same time, for those of ordinary skill in the art, according to the idea of the utility model, there will be changes in the specific implementation manners and application scopes. In summary, the content of this specification should not be construed as a limitation on the utility model.

Claims

1. A tetracycline detection device, characterized in that, include: Molecular imprinted membrane electrochemical sensor and portable electrochemical analysis device; The molecular imprinting membrane electrochemical sensor comprises: a screen-printed electrode and a membrane layer on the surface of a working electrode in the screen-printed electrode, wherein the membrane layer is a tetracycline molecular imprinting polymer membrane using graphene-gold nanoparticles synthesized by ultrasonic method as a modified material; when tetracycline is detected, the molecular imprinting membrane electrochemical sensor is connected to a portable electrochemical analysis device.

2. The tetracycline detection device according to claim 1, characterized in that The portable electrochemical analysis device comprises: A display screen, an electrochemical sensor interface, a housing, an analysis electrode arranged in the housing, a detection data storage chip, a microprocessor and a signal converter; the display screen is embedded in the housing, and the electrochemical sensor interface is embedded in one end of the housing; The electrochemical sensor interface, the analysis electrode, the detection data storage chip, the microprocessor, the signal converter and the display screen are connected in sequence.

3. The tetracycline detection device according to claim 2, characterized in that During tetracycline detection, one end of the molecular imprinted membrane electrochemical sensor is inserted into a solution in an electrolyte storage tank, and the other end of the molecular imprinted membrane electrochemical sensor is connected to the electrochemical sensor interface.

4. The tetracycline detection device according to claim 2, characterized in that Also includes: A sensor storage tank and an eluent storage tank, wherein the sensor storage tank and the eluent storage tank are both fixedly connected to the surface of the housing; The sensor storage tank is used to store the molecular imprinting membrane electrochemical sensor, and the eluent storage tank is used to store the eluent.

5. The tetracycline detection device according to claim 2, characterized in that: The housing also includes: a power supply; The power supply is respectively connected to the detection data storage chip, the microprocessor, the signal converter and the display screen.

6. The tetracycline detection device according to claim 2, characterized in that: The portable electrochemical analysis device further comprises: a voice reminder connected to the microprocessor; The voice reminder is used to announce the tetracycline concentration value and / or the operation status of the electrochemical analysis device.

7. The tetracycline detection device according to claim 2, characterized in that: A buckle is provided at one end of the shell side wall close to the electrochemical sensor interface; The buckle is used to fixedly connect the portable electrochemical analysis device to an electrolyte storage tank.

8. The tetracycline detection device according to claim 3, characterized in that: The solution in the electrolyte storage tank includes: a water sample and an electrolyte, wherein the electrolyte is a 0.1 mol / L polybutylene succinate solution, and the pH value of the electrolyte is 7.4; the electrolyte is used to dilute the water sample.

9. The tetracycline detection device according to claim 4, characterized in that: The eluent is a sodium hydroxide solution.

10. The tetracycline detection device according to claim 2, characterized in that: The housing of the portable electrochemical analysis device also includes: Bluetooth; the Bluetooth is connected to the signal converter.

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