Thermal cover assembly for PCR (polymerase chain reaction) and PCR analyzer

Abstract

The utility model relates to a thermal cover assembly for PCR reaction and a PCR analyzer, the PCR analyzer comprises the thermal cover assembly, the thermal cover assembly comprises a thermal cover and a sealing insertion plate, the edge of the thermal cover is provided with a buckle, the edge of the sealing insertion plate is provided with an insertion plate pin, the insertion plate pin is provided with a clamping groove with an opening, and the insertion plate pin can move relative to the sealing insertion plate. Therefore, the buckle can extend into the clamping groove from the opening of the clamping groove. After the plugboard pin is reset, the buckle can be clamped on the side wall of the clamping groove, so that the sealing plugboard can be fixedly mounted on the heat cover; and after the plugboard pin is moved again, the sealing plugboard can be conveniently taken down from the heat cover. Thus, on one hand, when the heat cover assembly is replaced, only the sealing insertion plate needs to be replaced, and therefore the maintenance cost can be reduced; on the other hand, a user can conveniently install the sealing insertion plate, so that the heat cover assembly has a very high error-tolerant rate, the sealing performance of a PCR experiment can be greatly improved, and evaporation of a test sample is effectively reduced.

Description

Technical Field

[0001] The present application relates to the technical field of biological detection, and particularly to a sealing cover plate for PCR reaction and a PCR analyzer. Background Art

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication in vitro, and its greatest feature is that it can greatly increase trace amounts of DNA. A PCR analyzer is an extremely important tool in molecular biology research and has been widely used in laboratories around the world. It can be applied to a variety of experimental applications, such as molecular cloning, gene expression analysis, genotyping, sequencing, and mutation.

[0003] Under normal circumstances, a thermal cover assembly is generally provided on a PCR analyzer for covering a full skirt plate containing a test sample in the PCR analyzer. The full skirt plate will be set in different temperature environments for temperature amplification experiments; and the thermal cover assembly can prevent the water vapor in the full skirt plate from condensing on the PCR tubes in the full skirt plate to ensure the stability of the reaction system and the efficient acquisition of reaction products. However, the thermal cover assemblies in existing PCR analyzers are usually integrally made and have a relatively complex structure. When replacing the thermal cover assembly, only the whole can be replaced, resulting in a relatively high maintenance cost; and the existing thermal cover assemblies are complex to install. Once installed improperly, it is impossible to effectively seal the test sample during the experiment and reduce its evaporation, resulting in a relatively large potential safety hazard. Summary of the Utility Model

[0004] Based on this, it is necessary to provide a thermal cover assembly for PCR reaction and a PCR analyzer including the thermal cover assembly, which have a simple structure and installation and can solve the above problems, aiming at the problems of the complex structure and installation of the thermal cover assembly in the existing PCR analyzer, resulting in a relatively high cost and the inability to effectively seal the test sample.

[0005] According to one aspect of the present application, a thermal cover assembly for PCR reaction is provided, including:

[0006] A thermal cover, one end edge of the thermal cover has a buckle;

[0007] A sealing plug board, one end edge of the sealing plug board along a first direction is provided with a plug board pin. The plug board pin is provided with a slot having an opening, and the plug board pin can move relative to the sealing plug board along a second direction perpendicular to the first direction, so that the buckle can extend into the slot from the opening of the slot, and after the plug board pin is reset, the buckle can be clamped to the slot wall of the slot, so that the sealing plug board can be fixedly installed on the thermal cover.

[0008] In one embodiment, the buckle includes a body and a clamping portion connected to each other. The clamping portion is bent from one end of the body along the second direction. The slot includes a first slot body and a second slot body that communicate with each other. The second slot body extends from one end of the first slot body along the second direction. The size of the first slot body in the second direction is greater than the size of the body in the second direction.

[0009] In one embodiment, a first support member and a second support member are provided at one end edge of the sealing plug plate. The first support member and the second support member are spaced apart in the second direction. The plug pin is movably inserted through the first support member, and one end of the plug pin is inserted into the second support member.

[0010] In one embodiment, an elastic element is provided in the second support member. One end of the plug pin inserted into the second support member abuts against the elastic element. The elastic element is configured to provide an elastic force for resetting the plug pin after moving along the second direction.

[0011] In one embodiment, the buckle has a first inclined surface, and the opening edge of the slot has a second inclined surface parallel to the first inclined surface. The first inclined surface is used to abut against the second inclined surface so that the plug pin can move relative to the buckle along the second direction.

[0012] In one embodiment, the thermal cover has sliding rails disposed oppositely at both ends in the second direction. Each sliding rail is provided with a chute having an opening facing the other sliding rail. The sealing plug plate is slidably limited at the edges of both ends in the second direction in the chute of a corresponding one of the sliding rails.

[0013] In one embodiment, a counterbore is provided on the side of the sealing plug plate facing away from the thermal cover, and a sealing gasket is embedded in the counterbore; the sealing plug plate is provided with an ejection hole penetrating through opposite sides thereof, and the ejection hole is used for inserting an ejector pin so that the ejector pin can separate the sealing gasket from the sealing plug plate.

[0014] According to another aspect of the present application, there is provided a PCR analyzer, including:

[0015] A base, on which a temperature control module is provided;

[0016] A full skirt plate, disposed above the temperature control module, and the full skirt plate is used for storing a sample to be tested;

[0017] An electric heat cover, comprising a heat cover assembly as described in any of the above schemes and two movable plates arranged relatively spaced apart along the second direction, the movable plates being slidably connected to a base and provided with an inclined groove inclined relative to the first direction, the heat cover assembly being provided with sliding pins at both ends of the second direction, the sliding pins being slidably limited in the inclined groove of one of the movable plates;

[0018] When the electric heat cover moves along the first direction, the sliding pin can slide in the inclined groove, so that the heat cover assembly can move relative to the moving plate along a vertical direction that is perpendicular to both the first direction and the second direction, so that it can cover the full skirt board or detach from the full skirt board.

[0019] In one of the embodiments, a limit pin is provided on one side of each movable plate facing the other movable plate, and the full skirt plate has step surfaces on two opposite sides in the second direction, respectively, and the step surfaces are located below the limit pin in the vertical direction. The limit pin is configured to abut against the step surface when the thermal cover assembly moves upward and is about to be separated from the full skirt plate, so as to prevent the full skirt plate from moving upward together with the thermal cover assembly.

[0020] In one embodiment, the base has a baffle at one end in the first direction, and the baffle is used to abut against the thermal cover assembly when the electric thermal cover moves a distance along the first direction, so that the thermal cover assembly can move relative to the moving plate.

[0021] The above-mentioned hot cover assembly and PCR analyzer for PCR reaction are provided with a hot cover and a sealing plug plate that are detachably connected to each other in the hot cover assembly, specifically, a buckle is provided on the edge of one end of the hot cover, and a plug plate pin that can move along a second direction perpendicular to the first direction is provided on the edge of one end of the sealing plug plate, and the plug plate pin is provided with a slot with an opening, so that when the plug plate pin moves along the second direction, the buckle can extend into the slot from the opening of the slot, and after the plug plate pin is reset, the buckle can be engaged with the slot wall of the slot, so that the sealing plug plate can be fixedly installed on the hot cover, and after the plug plate pin is moved again, the sealing plug plate can be conveniently removed from the hot cover. In this way, on the one hand, the hot cover assembly is not an integral structure, so when the hot cover assembly is replaced, only the sealing plug plate needs to be replaced, thereby reducing maintenance costs; on the other hand, the user can conveniently engage the sealing plug plate on one side of the hot cover without falling off, so that the hot cover assembly has a high fault tolerance rate, can greatly improve the sealing of the PCR experiment, and effectively reduce the evaporation of the test sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is an isometric view of a PCR analyzer provided in one embodiment of the present application.

[0023] Figure 2 A front view of a PCR analyzer provided in accordance with an embodiment of the present application.

[0024] Figure 3 for Figure 2 A magnified schematic diagram of area A in the middle.

[0025] Figure 4 An isometric view of a thermal cover assembly according to an embodiment of the present application.

[0026] Figure 5 Axial side view of a sealing insert in a thermal cover assembly provided in an embodiment of the present application Figure 1 .

[0027] Figure 6 for Figure 4 A magnified schematic diagram of area B.

[0028] Figure 7 for Figure 4 A magnified perspective view of area B.

[0029] Figure 8 The axial side view of the sealing plug plate in the thermal cover assembly of one embodiment of the present application Figure 2 .

[0030] Description of reference numerals:

[0031] 10. PCR analyzer; 100. Temperature control module; 200. Full skirt; 201. Step surface; 300. Electric hot cover; 310. Hot cover assembly; 311. Hot cover; 3111. Buckle; 3111a. Body; 3111b. Clamping part; 3111c. First inclined surface; 3112. Slide rail; 3112a. Slide groove; 312. Sealing plug plate; 312a. Sink; 3121. First support member; 3122. Second support member; 3123. Elastic element; 313. Board pin; 3131. Clamping groove; 3131a. First trough body; 3131b. Second trough body; 3131c. Second inclined surface; 320. Moving plate; 321. Inclined groove; 330. Sliding pin; 340. Limit pin. DETAILED DESCRIPTION

[0032] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0033] In the description of the present application, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or part referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0034] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0035] In this application, unless otherwise clearly specified and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two parts or the interaction relationship between two parts, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0036] In the present application, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0037] It should be noted that if a component is referred to as "fixed to" or "disposed on" another component, it can be directly on the other component or there may be an intermediate component. If a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be an intermediate component at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are only for illustrative purposes and do not represent the only implementation.

[0038] The present application provides a thermal cover assembly for PCR reaction and a PCR analyzer. The PCR analyzer includes a thermal cover assembly. The PCR analyzer is used to simulate the DNA replication process in vivo in vitro based on the amplification principle of polymerase chain reaction (PCR). The thermal cover assembly is used to cover the full skirt plate containing the test sample during the PCR reaction, so as to be able to seal the test sample and prevent the water vapor generated during the reaction from condensing inside the full skirt plate, thereby ensuring the smooth progress of the PCR reaction.

[0039] The structures of the PCR analyzer and the thermal cover assembly in the present application will be described below. It can be understood that in other embodiments, the thermal cover of the present application is not limited to being used in the PCR analyzer to seal the test sample in the full skirt plate, and can also be used to cover any other reaction plate containing the test sample to seal the test sample, which is not limited herein.

[0040] Refer to Figure 1 and Figure 2 , FIGS. 1 and Figure 2 show the PCR analyzer 10 in an embodiment of the present application. The PCR analyzer 10 provided by the embodiment of the present application includes a base (not shown in the figure), a temperature control module 100, a full skirt plate 200 and an electric thermal cover 300. The temperature control module 100 is disposed on the base, the full skirt plate 200 is disposed above the temperature control module 100, and the electric thermal cover 300 is slidably connected to the base. The base is used to play a supporting role in the entire PCR analyzer 10; the full skirt plate 200 is used to hold the test sample; the temperature control module 100 is used to heat the full skirt plate 200 to be able to heat the test sample contained in the full skirt plate 200 to different temperatures, so as to realize the rapid amplification of the target DNA fragment through the cycle of high-temperature denaturation, low-temperature annealing and appropriate-temperature extension; the electric thermal cover 300 can move relative to the base along the first direction (i.e., the X direction shown in the figure) to perform the actions of closing and opening the cover, so that the operator can perform secondary liquid addition or take out the full skirt plate 200. After the electric thermal cover 300 performs the cover closing action, the electric thermal cover 300 can seal the test sample contained in the full skirt plate 200 during the PCR reaction to keep the test sample in the full skirt plate 200 warm, prevent heat loss, and avoid the volatilization of the test sample into the air.

[0041] Specifically, the electric heat cover 300 includes a heat cover assembly 310 and two moving plates 320 that are relatively spaced apart in a second direction perpendicular to the first direction (i.e., the Y direction shown in the figure). The moving plates 320 are slidably connected to the base, and each moving plate 320 is provided with an inclined slot 321 that is inclined with respect to the first direction. In the direction from the inside to the outside in the figure, the inclined slot 321 is inclined upward away from the full skirt plate 200. Correspondingly, the heat cover assembly 310 is respectively provided with sliding pins 330 at both ends in the second direction, and the sliding pins 330 are slidably limited in the inclined slots 321 of the corresponding moving plate 320.

[0042] In this way, when the electric heat cover 300 moves in the first direction, the sliding pin 330 can slide in the inclined slot 321, so that the heat cover assembly 310 can move relative to the moving plate 320 in a vertical direction perpendicular to both the first direction and the second direction (i.e., the Z direction shown in the figure), so as to be able to cover or disengage from the full skirt plate 200.

[0043] Preferably, in order to enable the electric heat cover 300 to automatically perform the action of opening to disengage from the full skirt plate 200 or closing to press against the full skirt plate 200 while moving in the first direction, one end of the base in the first direction has a baffle (not shown in the figure), and the baffle is used to abut against the heat cover assembly 310 when the electric heat cover 300 moves outward in the first direction in the figure for a certain distance, so that the heat cover assembly 310 can move relative to the moving plate 320 to cover the full skirt plate 200.

[0044] More preferably, as shown in Figure 2 and Figure 3 , a limiting pin 340 is provided on one side of each moving plate 320 facing the other moving plate 320. The full skirt plate 200 respectively has a stepped surface 201 on opposite sides in the second direction, and the stepped surface 201 is located below the limiting pin 340 in the vertical direction. The limiting pin 340 is configured to abut against the stepped surface 201 when the heat cover assembly 310 moves upward and is about to disengage from the full skirt plate 200, so as to prevent the full skirt plate 200 from moving upward together with the heat cover assembly 310.

[0045] Thus, with the above settings, when the electric hot cover 300 moves in the first direction from outside the paper plane to inside the paper plane in the figure to perform the opening cover action so that the hot cover assembly 310 is about to disengage from the full skirt plate 200, since the limit pin 340 abuts against the step surface 201 of the full skirt plate 200, the detachment force between the hot cover assembly 310 and the full skirt plate 200 can be greater than the adsorption force between the hot cover assembly 310 and the full skirt plate 200. At this time, the hot cover assembly 310 and the full skirt plate 200 can be easily separated from each other, so that there is no need to forcibly separate the hot cover assembly 310 and the full skirt plate 200 artificially. Furthermore, the PCR analyzer 10 is stable and reliable during use. It can not only facilitate the secondary liquid addition to the full skirt plate 200, but also avoid damaging the full skirt plate 200 or splashing the test samples in the full skirt plate 200, resulting in sample contamination.

[0046] In the specific structure of the hot cover assembly 310, as Figure 4 and Figure 5 shown, the hot cover assembly 310 includes a hot cover 311 and a sealing plug plate 312. The sealing plug plate 312 is detachably connected to the side of the hot cover 311 facing the full skirt plate 200. The sealing plug plate 312 is used to cover the top surface of the full skirt plate 200 to seal the test samples in the full skirt plate 200. An electric heating wire is provided in the hot cover 311, which is used to heat the sealing cover plate when the sealing cover plate covers the full skirt plate 200, so that heat can be transferred to the top surface of the full skirt plate 200 through the sealing cover plate, preventing the test samples from evaporating into the air and preventing the test samples from condensing when encountering cold during the reaction process.

[0047] More specifically, the hot cover 311 has a buckle 3111 at one end edge along the first direction. The plug plate pin 313 is movably provided at one end of the sealing plug plate 312 along the first direction. The plug plate pin 313 is provided with a card slot 3131 having an opening, and the plug plate pin 313 can move relative to the sealing plug plate 312 along the second direction so that the buckle 3111 can extend into the card slot 3131 from the opening of the card slot 3131. After the plug plate pin 313 is reset, the buckle 3111 can be clamped to the side wall of the card slot 3131 so that the sealing plug plate 312 can be fixedly installed on the hot cover 311.

[0048] In one embodiment, as Figure 6As shown, the buckle 3111 is generally L-shaped and includes a body 3111a and a clamping portion 3111b that are connected to each other. The clamping portion 3111b is bent from one end of the body 3111a along the second direction. The clamping groove 3131 is also correspondingly generally L-shaped and includes a first groove body 3131a and a second groove body 3131b that are communicated with each other. The second groove body 3131b extends from the first groove body 3131a along the second direction. The dimension of the first groove body 3131a in the second direction is greater than the dimension of the body 3111a of the buckle 3111 in the second direction, so that the buckle 3111 can extend into the clamping groove 3131.

[0049] Further, in order to enable the plug pin 313 to be movably connected to the sealing plug 312, the sealing plug 312 is provided with a first support member 3121 and a second support member 3122 at one end along the first direction. The first support member 3121 and the second support member 3122 are spaced apart in the second direction. The plug pin 313 is movably inserted through the first support member 3121, and one end of the plug pin is inserted into the second support member 3122.

[0050] It can be understood that the first support member 3121 and the second support member 3122 can be integrally connected to the sealing cover plate, or can be connected to the sealing cover plate by screws as shown in the figure, which is not limited herein.

[0051] Furthermore, in order to enable the plug pin 313 to automatically reset after moving in the second direction relative to the sealing cover plate, as Figure 7 shown, an elastic element 3123 such as a spring is provided in the second support member 3122. One end of the plug pin 313 inserted into the second support member 3122 abuts against the elastic element 3123. The elastic element 3123 is configured to provide an elastic force for resetting the plug pin 313 after moving in the second direction.

[0052] In this way, when the plug pin 313 moves a certain distance in the second direction so that the buckle 3111 extends into the buckle 3111, under the action of the elastic force provided by the elastic element 3123, the plug pin 313 can move in the opposite direction and automatically reset, so that the clamping portion 3111b of the buckle 3111 abuts against the groove wall of the second groove body 3131b, thereby enabling the sealing cover plate to be firmly locked with the heat cover 311. When it is necessary to separate the two, similarly, as long as the plug pin 313 is moved a certain distance in the second direction, the buckle 3111 can be taken out from the clamping groove 3131 of the plug pin 313, so that the sealing cover plate can be conveniently separated from the heat cover 311.

[0053] Please continue to refer to Figure 6, as an improvement to the above embodiments, the engaging portion 3111b of the buckle 3111 has a first inclined surface 3111c, and the opening edge of the slot 3131 has a second inclined surface 3131c parallel to the first inclined surface 3111c. The first inclined surface 3111c is used to abut against the second inclined surface 3131c so that when the plug pin 313 contacts the buckle 3111, relative movement can occur between the first inclined surface 3111c and the second inclined surface 3131c. At this time, the plug pin 313 can automatically move relative to the buckle 3111 in the second direction. Therefore, there is no need to manually pull the plug pin 313 to move separately.

[0054] Please continue to refer to Figure 4 , in order to further facilitate the mutual insertion of the sealing cover plate and the thermal cover 311 and prevent the two from falling off, the thermal cover 311 has oppositely arranged slide rails 3112 at both ends in the second direction. Each slide rail 3112 is provided with a chute 3112a with an opening facing the other slide rail 3112. The edges at both ends of the sealing plug 312 in the second direction are respectively slidably limited in the chutes 3112a of the corresponding one of the slide rails 3112. In this way, when it is necessary to connect the sealing plug 312 and the thermal cover 311 to each other, only the edges at both ends of the sealing plug 312 need to be respectively inserted into the chutes 3112a of the corresponding one of the slide rails 3112, and then the sealing plug 312 is pushed in, so that the buckle 3111 and the plug pin 313 can contact each other. Furthermore, the buckle 3111 can extend into the slot 3131 of the plug pin 313 and abut against the slot wall of the slot 3131, so that the sealing plug 312 and the thermal cover 311 can be conveniently locked to each other; conversely, when it is necessary to remove the sealing plug 312 from the thermal cover 311 for replacement, as long as the plug pin 313 is slightly pulled and then the sealing plug 312 is pulled in the opposite direction, the sealing plug 312 and the thermal cover 311 can be separated from each other. Therefore, it can greatly facilitate the operation of the operator.

[0055] In addition, in order to enhance the sealing effect of the sealing cover plate, a sealing gasket (not shown in the figure) is provided on the side of the sealing plug 312 facing away from the thermal cover 311. One side of the sealing gasket fits against the sealing plate body, and the other side is used to fit against the top surface of the full skirt plate 200. And in order to better fix the sealing gasket on the sealing cover plate and further improve the sealing performance of the PCR experiment, thereby effectively reducing the evaporation of the sample, refer to Figure 8 , a sunk groove 312a is provided on the side of the sealing cover plate facing the full skirt plate 200, and a part of the sealing gasket is embedded in the sunk groove 312a. And furthermore, the sealing plug 312 is provided with an ejection hole (not shown in the figure) penetrating through its opposite sides. The ejection hole is used for inserting an ejector pin so that the sealing gasket can be separated from the sealing cover plate by using the ejector pin.

[0056] As can be seen, the thermal cover assembly 310 provided by the present application is not an integrally formed structure compared with the existing thermal cover assembly 310. Therefore, when replacing the thermal cover assembly 310, only the sealing plug 312 needs to be replaced, thereby reducing the maintenance cost. Moreover, the user can easily snap the sealing plug 312 on one side of the thermal cover 311 without falling off, making the thermal cover assembly 310 have a high fault tolerance rate, greatly improving the sealing performance of the PCR experiment, and effectively reducing the evaporation of the test sample. In addition, when the thermal cover assembly 310 is lifted upward and is about to be separated from the full skirt 200, since the limiting pin 340 is provided on the moving plate 320, the limiting pin 340 can abut against the stepped surface 201 of the full skirt 200, so that the full skirt 200 will not be lifted upward together with the thermal cover assembly 310. Furthermore, the PCR analyzer 10 is stable and reliable during use, effectively avoiding damage to the full skirt 200 or splashing of the test sample in the full skirt 200, resulting in sample contamination.

[0057] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0058] The above-described embodiments only represent several implementation manners of the present application, and the description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the appended claims.

Claims

1. A thermal cover assembly for PCR reaction, characterized in that: include: A thermal cover, wherein one end edge of the thermal cover along a first direction has a buckle; A sealing plug plate, wherein a plug plate pin is provided at one end edge of the sealing plug plate along the first direction, the plug plate pin is provided with a card slot with an opening, and the plug plate pin can move relative to the sealing plug plate along a second direction perpendicular to the first direction, so that the buckle can extend into the card slot from the opening of the card slot, and after the plug plate pin is reset, the buckle can be engaged with the groove wall of the card slot, so that the sealing plug plate can be fixedly installed on the thermal cover.

2. The thermal cover assembly according to claim 1, characterized in that The buckle includes a main body and a clamping portion that are connected to each other, the clamping portion is bent from one end of the main body along the second direction, the clamping slot includes a first slot body and a second slot body that are connected to each other, the second slot body extends from one end of the first slot body along the second direction, and the size of the first slot body in the second direction is larger than the size of the main body in the second direction.

3. The thermal cover assembly according to claim 1, characterized in that A first support member and a second support member are provided at one end edge of the sealing plug plate, the first support member and the second support member are spaced apart in the second direction, the plug plate pin can be movably inserted into the first support member, and one end of the plug plate pin is inserted into the second support member.

4. The thermal cover assembly according to claim 3, characterized in that: An elastic element is disposed in the second support member, one end of the latch pin inserted in the second support member abuts against the elastic element, and the elastic element is configured to provide an elastic force for restoring the latch pin after it moves along the second direction.

5. The thermal cover assembly according to claim 4, characterized in that The buckle has a first inclined surface, and the opening edge of the slot has a second inclined surface parallel to the first inclined surface. The first inclined surface is used to abut against the second inclined surface so that the latch pin can move relative to the buckle along the second direction.

6. The thermal cover assembly according to claim 1, characterized in that The heat cover has oppositely arranged slide rails at both ends in the second direction, each slide rail has a slide groove opening toward the other slide rail, and the two end edges of the sealing plug plate in the second direction are slidably limited in the slide groove of the corresponding one of the slide rails.

7. The thermal cover assembly according to claim 1, characterized in that The sealing plug plate is provided with a recessed groove on the side away from the heat cover, and a sealing gasket is embedded in the recessed groove; the sealing plug plate is provided with ejection holes penetrating through two opposite sides thereof, and the ejection holes are used for inserting an ejector pin so that the ejector pin can separate the sealing gasket from the sealing plug plate.

8. A PCR analyzer, characterized in that: include: A base, wherein a temperature control module is provided on the base; A full skirt board is provided on the temperature control module, and the full skirt board is used to store samples to be tested; An electric heat cover, comprising a heat cover assembly according to any one of claims 1 to 7 and two movable plates arranged relatively spaced apart along the second direction, the movable plates being slidably connected to a base and provided with an inclined groove inclined relative to the first direction, the heat cover assembly being provided with sliding pins at both ends of the second direction, the sliding pins being slidably limited in the inclined groove of one of the movable plates; When the electric heat cover moves along the first direction, the sliding pin can slide in the inclined groove, so that the heat cover assembly can move relative to the moving plate along a vertical direction that is perpendicular to both the first direction and the second direction, so that it can cover the full skirt board or detach from the full skirt board.

9. The PCR analyzer according to claim 8, characterized in that: A limit pin is provided on one side of each movable plate facing the other movable plate, and the full skirt plate has step surfaces on two opposite sides in the second direction, respectively, and the step surfaces are located below the limit pin in the vertical direction. The limit pin is configured to abut against the step surface when the thermal cover assembly moves upward and is about to be separated from the full skirt plate, so as to prevent the full skirt plate from moving upward together with the thermal cover assembly.

10. The PCR analyzer according to claim 8, characterized in that: The base has a baffle at one end in the first direction, and the baffle is used to abut against the thermal cover assembly when the electric thermal cover moves a distance along the first direction, so that the thermal cover assembly can move relative to the moving plate.

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