Inoculation device of strain fermentation tank

Abstract

The utility model provides a strain fermentation tank inoculation device which comprises a barrel and a flame ring, the barrel can be arranged on an inoculation opening in a sleeving mode, a sleeve is fixed to a fermentation tank, a net ring is fixed to the inner wall of the barrel, the flame ring is annularly fixed to the upper end of the net ring, and a conical cover integrally formed with the barrel is arranged at the upper end of the barrel. The top of the conical cover is provided with a through hole capable of pouring bacterial liquid for inoculation through the conical flask. According to the inoculation device for the strain fermentation tank, when a flame inoculation method is used for strain inoculation, flames generated by igniting the flame ring are limited in the barrel, the effects that the flames cover the inoculation opening to keep a sterile environment and prevent infectious microbes from entering the fermentation tank are achieved, diffusion of surrounding flames is limited through the barrel and the conical cover, and the inoculation effect is improved. And the risk that operators are scalded is reduced.

Description

Technical Field

[0001] This application relates to the technology of strain expansion culture inoculation, and particularly to a strain fermenter inoculation device. Background Art

[0002] When producing and culturing strains, it is necessary to first load the mixed medium into the fermenter, and then perform steam sterilization on the medium through high-temperature steam. When inoculating, the bacterial liquid is poured into the fermenter through the inoculation port.

[0003] In the existing inoculation process, it is necessary to create a sterile environment when pouring the bacterial liquid into the inoculation port to prevent miscellaneous bacteria from entering the fermenter through the inoculation port. The usual flame inoculation method is to soak the flame ring with alcohol and then place it around the inoculation port, and then light the flame ring. After the burning flame covers the inoculation port, the inoculation port cover is opened in the flame. After disinfecting the flame at the mouth of the conical flask, the mouth of the conical flask is passed through the flame, and the bacterial liquid is quickly poured into the fermenter.

[0004] By covering the inoculation port with the flame, a sterile environment can be maintained at the inoculation port. In the existing inoculation process, the flame generated by the burning of the flame ring has no shielding limit. Although the operator wears fireproof gloves during the operation, there is still a risk of being scalded, and safety accidents are likely to occur. Summary of the Utility Model

[0005] This application provides a strain fermenter inoculation device to solve the problem that in the existing flame inoculation, the flame generated by the burning of the flame ring cannot be restricted and safety accidents are likely to occur.

[0006] This application provides a strain fermenter inoculation device, including a cylinder body and a flame ring. The cylinder body can be sleeved on the inoculation port, and the sleeve is fixed to the fermenter. A mesh ring is fixed on the inner wall of the cylinder body, and the flame ring is fixedly arranged at the upper end of the mesh ring. A conical cover integrally formed with the cylinder body is provided at the upper end of the cylinder body, and a through hole through which the bacterial liquid can be poured for inoculation by tipping the conical flask is provided at the top of the conical cover.

[0007] Optionally, a sleeve slidably connected to the cylinder body is sleeved at the lower end of the cylinder body. A spring for jacking it upward is fixed at the bottom of the cylinder body, and a pressing ring for pressing down is fixed on the side wall of the cylinder body.

[0008] Optionally, a sealing gasket fitting the fermenter is fixed at the bottom of the sleeve.

[0009] Optionally, a cover body for closing and extinguishing the fire is fixed on the through hole at the top of the conical cover.

[0010] Optionally, the cover body is an annular structure with a variable aperture by rotating a plurality of hinged blades.

[0011] Optionally, a guide rail is vertically fixed to the inner wall of the cylinder body. The net ring is slidably connected to the guide rail in the vertical direction through a slider, and a bolt for fixing it is threadedly connected to the slider.

[0012] Compared with the prior art, the beneficial effects of the strain fermentation tank inoculation device provided by the present application are as follows:

[0013] When inoculating strains by the flame inoculation method, the flame generated by igniting the flame ring is restricted inside the cylinder body, which not only achieves the effect of covering the inoculation port with the flame to maintain a sterile environment and prevent contaminants from entering the fermentation tank, but also restricts the spread of the surrounding flame through the cylinder body and the conical cover, reducing the risk of scalding the operator. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0015] Figure 1 The front view of the strain fermentation tank inoculation device provided by an embodiment of the present application;

[0016] Figure 2 The strain fermentation tank inoculation device provided by an embodiment of the present application Figure 1 The sectional view;

[0017] Figure 3 The enlarged view at A of the strain fermentation tank inoculation device provided by an embodiment of the present application;

[0018] Figure 4 The installation and use schematic diagram of the strain fermentation tank inoculation device provided by an embodiment of the present application;

[0019] Figure 5 The schematic diagram of the cover body with variable aperture of the strain fermentation tank inoculation device provided by an embodiment of the present application;

[0020] Figure 6 The strain fermentation tank inoculation device provided by an embodiment of the present application Figure 5 The schematic diagram after the aperture is changed.

[0021] Description of the reference numerals:

[0022] Cylinder body 1; net ring 2; flame ring 3; conical cover 4; sleeve 5; sealing gasket 6; spring 7; pressing ring 8; cover body 9; blade 10; guide rail 11; slider 12; bolt 13; fermentation tank 14; inoculation port 15; inoculation port cover 16; opening wrench 17. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be described clearly and completely below. Apparently, the described embodiments are some, rather than all, of the embodiments of this application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in this application without making creative efforts also belong to the scope of protection of this application.

[0024] As Figures 1 - 4 shown, an inoculation device for a strain fermenter according to an embodiment of this application includes a cylinder body 1 and a flame ring 3. The cylinder body 1 can be sleeved on the inoculation port 15. The sleeve 5 is fixed to the fermenter 14. A net ring 2 is fixed to the inner wall of the cylinder body 1. The flame ring 3 is fixed annularly above the net ring 2. A conical cover 4 integrally formed with the cylinder body 1 is provided at the upper end of the cylinder body 1. A through hole through which a conical flask can be tilted to pour the bacterial liquid for inoculation is provided at the top of the conical cover 4.

[0025] During inoculation, first, the air pressure in the fermenter 14 is reduced. The flame ring 3 soaked in alcohol is fixed on the top surface of the net ring 2, and then the cylinder body 1 is sleeved on the inoculation port 15 of the fermenter 14. The bottom of the cylinder body 1 is placed on the fermenter 14 so that the inoculation port 15 is aligned with the through hole at the upper end of the conical cover 4, and the flame ring 3 surrounds the periphery of the inoculation port 15.

[0026] The flame ring 3 is ignited from the through hole, and the flame covers the mouth of the inoculation port 15. The inoculation port cover 16 is opened in the flame by the opening wrench 17. The conical flask containing the bacterial liquid is opened, and the mouth of the conical flask is quickly flame-sterilized. Then, the bacterial liquid in the conical flask is poured into the fermenter 14 through the inoculation port 15 in the flame. After the inoculation is completed, the inoculation port cover 16 is quickly covered back. After confirming that the inoculation port cover 16 is tightly closed, the through hole of the conical cover 4 is blocked to extinguish the flame ring 3, and the cylinder body 1 is removed.

[0027] In this embodiment, when inoculating the strain by the flame inoculation method, the flame generated by igniting the flame ring 3 is restricted within the cylinder body 1, which not only achieves the effect of covering the inoculation port 15 with the flame to maintain a sterile environment and prevent foreign bacteria from entering the fermenter 14, but also restricts the diffusion of the surrounding flame through the cylinder body 1 and the conical cover 4, reducing the risk of scalding the operator.

[0028] In a possible implementation, a sleeve 5 slidably connected to the cylinder body 1 is sleeved on the lower end of the cylinder body 1. A spring 7 for jacking it upward is fixed to the bottom of the cylinder body 1. A pressing ring 8 for pressing downward is fixed to the side wall of the cylinder body 1.

[0029] Under normal conditions during inoculation, the cylinder body 1 is pushed up and extended by the spring 7. When pouring the bacterial liquid into the fermentation tank 14 through an Erlenmeyer flask, pressing down the cylinder body 1 through the pressing ring 8 can move the cylinder body 1 and the flame ring 3 downward, making the mouth of the Erlenmeyer flask closer to the mouth of the inoculation port 15, which is convenient for accurate operation.

[0030] In a possible implementation manner, a sealing gasket 6 that fits the fermentation tank 14 is fixed at the bottom of the sleeve 5.

[0031] The sealing gasket 6 made of rubber material can make the sleeve 5 fit more tightly and seal when placed on the fermentation tank 14. When it is convenient to block and extinguish the fire through the through hole at the upper end of the conical cover 4, a closed space is formed inside the cylinder body 1 and the sleeve 5 to isolate the air for fire extinguishing.

[0032] In a possible implementation manner, a cover body 9 for closing and extinguishing the fire is fixed on the through hole at the top of the conical cover 4.

[0033] After completing the inoculation and covering the inoculation port cover 16, the through hole at the top of the conical cover 4 is closed through the cover body 9. After closing, the cylinder body 1 and the sleeve 5 form a sealed space to isolate the air and extinguish the flame ring 3.

[0034] As Figure 5 、 Figure 6 shown, in a possible implementation manner, the cover body 9 is an annular structure with a variable aperture formed by rotating a plurality of hinged blades 10.

[0035] The cover body 9 with a variable aperture can adjust the aperture by rotating the hinged blades 10 to match the diameter of the inoculation port 15 for easy pouring. At the same time, it is adjusted according to the situation during inoculation to further limit the flames drilled out of the through hole at the top of the conical cover 4 and improve the protection effect.

[0036] In a possible implementation manner, a guide rail 11 is vertically fixed on the inner wall of the cylinder body 1. The mesh ring 2 is slidably connected to the guide rail 11 in the vertical direction through a slider 12, and a bolt 13 for fixing it is threadedly connected to the slider 12.

[0037] The height position of the mesh ring 2 can be adjusted vertically along the guide rail 11 through the slider 12, thereby controlling the height of the flame ring 3. It can be flexibly adjusted according to the length of different inoculation ports 15 to ensure that the flame covers the inoculation port 15 and maintain the effect of a sterile environment.

[0038] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than limiting them; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A bacterial strain fermentation tank inoculation device, comprising a barrel (1) and a flame ring (3), wherein the barrel (1) can be sleeved on an inoculation port (15), and the sleeve (5) is fixed to the fermentation tank (14), characterized in that: A mesh ring (2) is fixed on the inner wall of the cylinder (1), the flame ring (3) is annularly fixed on the upper end of the mesh ring (2), the upper end of the cylinder (1) is provided with a conical cover (4) integrally formed therewith, the top of the conical cover (4) is provided with a through hole capable of pouring bacterial liquid for inoculation through a conical flask, the lower end of the cylinder (1) is sleeved with a sleeve (5) slidably connected thereto, the bottom of the cylinder (1) is fixed with a spring (7) for lifting it upward, the side wall of the cylinder (1) is fixed with a pressure ring (8) for pressing downward, and the bottom of the sleeve (5) is fixed with a sealing gasket (6) that fits the fermentation tank (14).

2. The bacterial strain fermentation tank inoculation device according to claim 1, characterized in that: A cover body (9) for sealing and extinguishing fire is fixed to the through hole at the top of the conical cover (4).

3. The bacterial strain fermentation tank inoculation device according to claim 2, characterized in that: The cover body (9) is an annular structure whose aperture can be changed by rotating a plurality of hinged blades (10).

4. The bacterial strain fermentation tank inoculation device according to any one of claims 1 to 3, characterized in that: A guide rail (11) is vertically fixed to the inner wall of the cylinder (1), and the net ring (2) is slidably connected to the guide rail (11) in the vertical direction via a slider (12), and a bolt (13) for fixing the slider (12) is threadedly connected to the slider (12).

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