Efficient culture device for microorganisms

By designing a high-efficiency microbial culture device, and utilizing an inoculation shell and a tilting mechanism to achieve efficient inoculation under sterile conditions, the problem of multi-person coordination was solved, ensuring the efficient culture of Bacillus coagulans.

CN121450409APending Publication Date: 2026-02-03JIANGSU FOOD & PHARMA SCI COLLEGE
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Patent Information

Application Number
CN202511604611.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-11-05
Publication Date
2026-02-03

AI Technical Summary

Technical Problem

When inoculating Bacillus coagulans cultured in the laboratory into a large fermenter, multiple people need to coordinate and cooperate. Errors can easily lead to the bacterial solution being exposed to a non-sterile environment, affecting the culture results.

Method used

A highly efficient microbial culture device was designed, comprising an inoculation shell, an opening mechanism, and a microbial tilting mechanism. Through the coordinated operation of the fire ring, the opening mechanism, and the tilting plate, efficient inoculation under sterile conditions is achieved.

Benefits of technology

This technology enables efficient inoculation of microorganisms in a sterile environment, avoiding bacterial contamination caused by human error and ensuring efficient microbial cultivation.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the technical field of efficient culture of microorganisms, in particular to an efficient culture device of microorganisms, which comprises a culture tank body, a pouring-in pipe arranged on the culture tank body and a sealing cover arranged on the pouring-in pipe, and a guide-in shell is arranged on the outer side of the pouring-in pipe. The guiding-in shell is arranged on the sealing cover, the putting-in opening where the fire ring can be conveniently placed is formed in the guiding-in shell, then the fire ring can be placed at the guiding-in shell, the uncovering mechanism is arranged on the guiding-in shell, then the effect of opening the sealing cover is achieved, and when the sealing cover is opened, the microorganism pouring mechanism is arranged, so that the fire ring can be conveniently poured out. Therefore, the bottle opening of the culture bottle filled with microorganisms can be slowly rotated to the fire ring, meanwhile, the bottle plug is opened, efficient inoculation in a sterile environment is achieved, meanwhile, through high-precision cooperation of a plurality of mechanisms, the phenomena that the bottle plug is opened too slowly or the dumping angle is improper and the like are avoided, and the inoculation efficiency is improved. The effect of efficiently culturing the microorganisms is further realized.
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Description

Technical Field

[0001] This invention relates to the field of high-efficiency microbial culture technology, specifically to a high-efficiency microbial culture device. Background Technology

[0002] Bacillus coagulans is a type of microorganism, specifically belonging to the genus Bacillus in the phylum Firmicutes. It is a Gram-positive bacterium that combines the characteristics of both lactic acid bacteria and Bacillus, making it an excellent probiotic. In industrial production, Bacillus coagulans is first cultured on a small scale in a laboratory using culture flasks. Subsequently, the cultured Bacillus coagulans is inoculated into a fermenter for large-scale cultivation. By precisely controlling the temperature and dissolved oxygen levels in the fermenter, the activity of the bacteria can be maintained while avoiding excessive spore formation that could lead to a decrease in metabolic activity.

[0003] However, when culturing Bacillus coagulans in the laboratory into a large fermenter, to ensure a sterile environment for inoculation, a fire ring made of alcohol swabs is placed over the inlet of the fermenter. One person holds the handle of the fire ring while simultaneously opening the sealed cap of the inlet, while another person slowly pours the culture bottle. During this pouring, another person must open the stopper. With the fire ring burning, Bacillus coagulans is aseptically poured from the culture bottle into the fermenter. This process requires simultaneous coordination among multiple people. Any delay or error by any person (such as opening the stopper too slowly or pouring at an improper angle) will expose the bacterial solution to a non-sterile environment, further affecting the cultivation of Bacillus coagulans. Therefore, we propose a highly efficient microbial culture device. Summary of the Invention

[0004] The purpose of this invention is to provide a highly efficient microbial culture device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a high-efficiency microbial culture device, comprising a culture tank body, an inlet tube disposed on the culture tank body, and a sealing cap disposed on the inlet tube, wherein an inlet shell is provided on the outside of the inlet tube, and the bottom of the inlet shell is fixedly installed on the culture tank body;

[0006] The inlet shell is provided with an inlet, and a handle is provided at the bottom of the inlet. One end of the handle is connected to a fire ring, which is sleeved on the outside of the pouring tube.

[0007] The top of the inlet shell is provided with an opening mechanism, which is used to open the sealing cover;

[0008] A fixed frame is fixedly connected to one side of the bottom of the inlet shell. A tilting plate is rotatably connected to the fixed frame. A microbial tilting mechanism is provided on the tilting plate, and a driving component for driving the tilting plate is provided on the fixed frame. The microbial tilting mechanism cooperates with the opening mechanism to pour microorganisms into the interior of the culture tank body through the pouring tube.

[0009] Furthermore, the opening mechanism includes a support rod, a movable shell, a clamping component, a telescopic connector, and a rotating component. The support rod slides through the top of the guide shell, the movable shell is located inside the guide shell, and the movable shell is fixedly installed at the bottom of the support rod. The clamping component is located inside the movable shell and is used to clamp and fix the sealing cover.

[0010] The telescopic connector is located on the outer side of the top of the inlet shell and is used to connect the support rod. The rotating component is located on the outer side of the inlet shell and is used to drive the support rod. Through the provided opening mechanism, the sealing cover can be opened.

[0011] Furthermore, the clamping component includes a heat insulation plate, an electric push rod, a push shell, a push bracket, a push support rod, and a contact element. The heat insulation plate is fixedly installed inside the movable shell, and the electric push rod is fixedly installed at the top inside the movable shell. The output end of the electric push rod passes through the heat insulation plate and is fixedly connected to the push shell, and the output end of the electric push rod is slidably connected to the heat insulation plate.

[0012] The push bracket is provided in multiple ways, and the multiple push brackets are arranged around the bottom of the push shell. One end of the push support rod is rotatably connected to the push bracket, and the other end of the push bracket is connected to the contact member. The contact member is slidably connected to the bottom of the movable shell, and the multiple contact members lock and fix the sealing cover together. The sealing cover is clamped by the provided clamping member.

[0013] Furthermore, the contact element includes a movable frame and a clamping plate. The movable frame is slidably connected to the bottom of the movable shell, and one end of the push rod is slidably connected to the movable frame. The clamping plate is fixedly installed at the bottom of the movable frame, and one end of the bottom of the clamping plate is the clamping end. Through the provided contact element, the function of contacting and connecting the sealing cover is realized.

[0014] Furthermore, the inlet shell has an open opening on one side corresponding to the microbial pouring mechanism.

[0015] Furthermore, the microbial pouring mechanism includes a culture bottle, a stopper, a pull-out mechanism, and a fixing mechanism. The culture bottle is placed on the pouring plate, and the stopper is fitted at the mouth of the culture bottle. The pull-out mechanism is installed on the pouring plate and slowly pulls out the stopper when the culture bottle is poured towards the pouring tube. The fixing mechanism is installed on the pouring plate and is used to fix the culture bottle. Through the provided microbial pouring mechanism, the microorganisms in the culture bottle are slowly and efficiently poured into the interior of the culture tank.

[0016] Furthermore, the tilting plate is L-shaped and has an annular groove, which is used to limit the bottom of the culture bottle.

[0017] Furthermore, the pull-out mechanism includes a fixed side plate, a support member, a fixed top plate, and a pushing member. The fixed side plate is located on the outer side of the top of the culture bottle. The support member is installed on the tilting plate and is used to support the fixed side plate. The fixed top plate is provided with a fixing port, and the fixed top plate is sleeved on the outside of the bottle stopper through the fixing port. The fixed top plate is provided with two locking bolts for locking the bottle stopper.

[0018] The pusher is mounted on the fixed side plate and is used to drive the fixed top plate. Through the provided pull-out mechanism, the bottle stopper can be pulled out.

[0019] Furthermore, the pushing component includes a guide plate, a connecting pushing plate, a drive shaft, a rotating component, and a tensioning component. The guide plate is fixedly mounted on a fixed side plate and has a guide groove. A first guide rod and a second guide rod slide through the guide groove. There are two connecting pushing plates, which are located on both sides of the guide plate. The two ends of the first guide rod and the second guide rod are fixedly connected to the two connecting pushing plates.

[0020] Two connecting push plates are fixedly installed at the bottom of the fixed top plate. The drive shaft is rotatably connected to the fixed side plate, and the rotating component is used to drive the drive shaft. Two tensioning components are provided. One end of each tensioning component is connected to the drive shaft, and the other end of the tensioning component is connected to the connecting push plate. Through the provided pushing components, the fixed top plate is pushed.

[0021] Furthermore, the tensioning member includes a first rotating support rod and a second rotating support rod. One end of the first rotating support rod is fixedly sleeved on the outside of the drive shaft, and the other end of the first rotating support rod is rotatably connected to the second rotating support rod through a pin. The other end of the second rotating support rod is rotatably connected to the connecting push plate through a pin. Through the tensioning member, the function of tensioning and connecting the connecting push plate is realized.

[0022] The present invention has at least the following beneficial effects:

[0023] In use, this invention utilizes an inlet shell on the outside of the infusion tube, with an opening for placing a flame ring. The flame ring can then be placed in the inlet shell, which has an opening mechanism to open the sealed cap. Simultaneously, a microbial pouring mechanism slowly rotates the mouth of the culture bottle containing microorganisms towards the flame ring, opening the stopper. This allows for efficient inoculation under sterile conditions. Furthermore, the high-precision coordination of multiple mechanisms prevents issues such as slow stopper opening or improper pouring angles, further enhancing the efficient cultivation of microorganisms. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0025] Figure 2 This is a perspective view of the overall structure of the present invention;

[0026] Figure 3 This is a schematic diagram of the shell structure introduced in this invention;

[0027] Figure 4 This is a schematic diagram of the open structure of the present invention;

[0028] Figure 5 This is a schematic diagram of the fire ring structure of the present invention;

[0029] Figure 6 This is a schematic diagram of the clamping component structure of the present invention;

[0030] Figure 7 This is a schematic diagram of the clamping plate structure of the present invention;

[0031] Figure 8 This is a schematic diagram of the tilting plate structure of the present invention;

[0032] Figure 9 This is a schematic diagram of the culture flask structure of the present invention;

[0033] Figure 10 This is a schematic diagram of the pull-out mechanism of the present invention;

[0034] Figure 11 This is a schematic diagram of the tensioning component structure of the present invention;

[0035] Figure 12 This is a schematic diagram of the connecting push plate structure of the present invention;

[0036] Figure 13 This is a schematic diagram of the guide plate structure of the present invention;

[0037] Figure 14This is a side view of the guide plate structure of the present invention;

[0038] Figure 15 This is a schematic diagram of the culture flask structure in the tilted state of the present invention.

[0039] In the diagram: 1-Culturation tank body; 2-Pour-in tube; 21-Sealing cap; 3-Inlet shell; 31-Inlet; 32-Open opening; 4-Handle; 41-Fire ring; 5-Opening mechanism; 51-Support rod; 52-Moving shell; 53-Clamping component; 531-Heat insulation plate; 532-Electric push rod; 533-Push shell; 534-Push bracket; 535-Push support rod; 536-Contact component; 5361-Moving frame; 5362-Clamping plate; 5363-Clamping end; 54-Telescopic connector; 541-Telescopic cylinder; 542-Connecting plate; 55-Rotating component; 551-Rotating sleeve; 552-Rotating gear assembly; 6-Fixed frame; 61-Tilting plate; 611-Annular groove; 7-Microbial tilting mechanism; 71-Cultivation flask; 72-Bottler; 73-Pull-out mechanism; 731-Fixed side plate; 732-Support component; 733-Fixed top plate; 734-Push component; 7341-Guide plate; 7342-Connecting push plate; 7343-Drive shaft; 735-Locking bolt; 74-Fixing mechanism; 741-Double threaded rod; 742-Handle; 743-Moving block; 744-Fixed rod; 745-Fixed plate; 75-Rotating component; 76-Tensioning component; 761-First rotating support rod; 762-Second rotating support rod; 77-Guide groove; 78-First guide rod; 79-Second guide rod; 8-Drive component. Detailed Implementation

[0040] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0041] Example 1

[0042] Please see Figures 1 to 2 A highly efficient microbial culture device includes a culture tank body 1, an inlet tube 2 disposed on the culture tank body 1, and a sealing cap 21 disposed on the inlet tube 2. An inlet shell 3 is provided on the outside of the inlet tube 2, and the bottom of the inlet shell 3 is fixedly installed on the culture tank body 1. In this application, the inlet shell 3 is spirally and detachably installed on the culture tank body 1, which facilitates the disassembly of the inlet shell 3 and facilitates inoculation in different culture tank bodies 1.

[0043] Please see Figures 2 to 5The inlet shell 3 is provided with an inlet 31, and the bottom of the inlet 31 is provided with a handle 4. An extension plate is provided on the outside of the inlet 31, and the extension plate is provided with a groove for supporting the handle 4.

[0044] One end of the handle 4 is connected to a fire ring 41, which is fitted onto the outside of the pouring pipe 2.

[0045] Please see Figures 5 to 7 The top of the inlet shell 3 is provided with an opening mechanism 5, which is used to open the sealing cover 21. The opening mechanism 5 includes a support rod 51, a movable shell 52, a clamping member 53, a telescopic connecting member 54, and a rotating member 55. The support rod 51 slides through the top of the inlet shell 3. The movable shell 52 is located inside the inlet shell 3 and is fixedly installed at the bottom of the support rod 51. The clamping member 53 is located inside the movable shell 52 and is used to clamp and fix the sealing cover 21.

[0046] The telescopic connector 54 is located on the outer side of the top of the inlet shell 3 and is used to connect the support rod 51. The rotating part 55 is located on the outer side of the inlet shell 3 and is used to drive the support rod 51.

[0047] The clamping member 53 includes a heat insulation plate 531, an electric push rod 532, a push housing 533, a push bracket 534, a push support rod 535, and a contact member 536. The heat insulation plate 531 is fixedly installed inside the movable housing 52, and the electric push rod 532 is fixedly installed at the top inside the movable housing 52. The output end of the electric push rod 532 passes through the heat insulation plate 531 and is fixedly connected to the push housing 533, and the output end of the electric push rod 532 is slidably connected to the heat insulation plate 531.

[0048] Multiple push brackets 534 are provided, and multiple push brackets 534 are arranged around the bottom of push housing 533. One end of push support rod 535 is rotatably connected to push bracket 534, and the other end of push bracket 534 is connected to contact member 536. Contact member 536 is slidably connected to the bottom of movable housing 52, and multiple contact members 536 lock and fix the sealing cover 21 to each other.

[0049] The contact element 536 includes a movable frame 5361 and a clamping plate 5362. The movable frame 5361 is slidably connected to the bottom of the movable housing 52, and one end of the push rod 535 is slidably connected to the movable frame 5361. In this application, the movable frame 5361 is provided with a guide groove, and the bottom of the movable housing 52 is provided with a guide rail at the position corresponding to the guide groove. The guide rail and the guide groove are used to limit and guide the movable frame 5361.

[0050] The clamping plate 5362 is fixedly installed at the bottom of the movable frame 5361, and one end of the bottom of the clamping plate 5362 is the clamping end 5363.

[0051] Specific implementation process: When inoculating the inside of the culture tank body 1 with microorganisms such as Bacillus coagulans, the fire ring 41 is first placed into the inlet shell 3 through the inlet 31. The burning fire ring 41 is fitted on the outside of the pouring tube 2. At the same time, the handle 4 on the fire ring 41 is supported by the extension plate at the inlet 31.

[0052] Subsequently, the telescopic connector 54 operates, causing the movable shell 52 to move downwards along the interior of the guide shell 3 until the multiple clamping plates 5362 at the bottom of the movable shell 52 have all moved to the outside of the sealing cover 21. Then, the electric push rod 532 operates, causing the push shell 533 to drive the multiple push brackets 534 to move upwards along the interior of the movable shell 52. As the push brackets 534 move upwards, they simultaneously drive the push support rod 535 to move. The push support rod 535 drives the movable frame 5361, which in turn causes the clamping plates 5362 at the movable frame 5361 to move along the direction of the sealing cover 21 until the clamping ends 5363 of the multiple clamping plates 5362 clamp and fix the outside of the sealing cover 21, thereby achieving the function of fixing the sealing cover 21.

[0053] At this time, the rotating component 55 operates, thereby fixing the sealing cover 21 with multiple clamping plates 5362 while rotating the sealing cover 21. At the same time, the telescopic connecting component 54 operates, causing the sealing cover 21 to disengage from the pouring pipe 2 and then move vertically upward along the pouring pipe 2.

[0054] As a further explanation, the telescopic connector 54 includes a telescopic cylinder 541 and a connecting plate 542. The telescopic cylinder 541 is fixedly installed on the outer side of the top of the inlet shell 3, and the output end of the telescopic cylinder 541 is fixedly connected to the connecting plate 542. The support rod 51 is rotatably connected to the bottom of the connecting plate 542.

[0055] Then, by operating the output end of the telescopic cylinder 541, the connecting plate 542 drives the support rod 51 to move upward or downward relative to the inlet shell 3.

[0056] The rotating component 55 includes a rotating sleeve 551 and a rotating gear assembly 552. The rotating sleeve 551 is rotatably connected to the top of the inlet shell 3. A support rod 51 slides through the rotating sleeve 551 and has two synchronization grooves. A synchronization guide bar is provided on the rotating sleeve 551 at the position corresponding to the synchronization groove, and the synchronization guide bar is slidably connected to the synchronization groove. The rotating gear assembly 552 is located on the top of the inlet shell 3 and is used to drive the rotating sleeve 551. In this application, the rotating gear assembly 552 is composed of a motor, gears, and a gear chain. For details, please refer to the appendix of the specification. Figure 4 .

[0057] Please see Figures 8 to 15A fixed frame 6 is fixedly connected to one side of the bottom of the inlet shell 3. A tilting plate 61 is rotatably connected to the fixed frame 6. The tilting plate 61 is L-shaped and has an annular groove 611. The annular groove 611 is used to limit the bottom of the culture bottle 71. A microbial tilting mechanism 7 is provided on the tilting plate 61. A driving component 8 for driving the tilting plate 61 is provided on the fixed frame 6. As a further supplementary explanation, the driving component 8 includes a push cylinder. The push cylinder is rotatably connected to the bottom of the fixed frame 6 through a hinge support. The output end of the push cylinder is rotatably connected to the tilting plate 61 through the hinge support.

[0058] The microbial pouring mechanism 7 and the lid opening mechanism 5 work together to pour microorganisms from the pouring tube 2 into the interior of the culture tank body 1.

[0059] An opening 32 is provided on one side of the import shell 3 corresponding to the microbial pouring mechanism 7.

[0060] The microbial pouring mechanism 7 includes a culture bottle 71, a stopper 72, a pull-out mechanism 73, and a fixing mechanism 74. The culture bottle 71 is mounted on the pouring plate 61, and the stopper 72 is fitted at the mouth of the culture bottle 71. The pull-out mechanism 73 is mounted on the pouring plate 61, and when the culture bottle 71 is poured toward the pouring tube 2, the pull-out mechanism 73 slowly pulls out the stopper 72. The fixing mechanism 74 is mounted on the pouring plate 61 and is used to fix the culture bottle 71.

[0061] The pull-out mechanism 73 includes a fixed side plate 731, a support member 732, a fixed top plate 733, and a pusher member 734. The fixed side plate 731 is located on the outer side of the top of the culture bottle 71. The support member 732 is installed on the tilting plate 61 and is used to support the fixed side plate 731. The fixed top plate 733 is provided with a fixing port and is fitted onto the outside of the bottle stopper 72 through the fixing port. The fixed top plate 733 is provided with two locking bolts 735 for locking the bottle stopper 72.

[0062] As a further explanation, the support member 732 includes a support shell and a support cylinder. The support shell is fixedly installed on the fixed frame 6, and the support cylinder is fixedly installed inside the support shell. The output end of the support cylinder passes through the support shell and is fixedly connected to the fixed side plate 731, and the output end of the support cylinder is slidably connected to the support shell.

[0063] When the culture flask 71 is fixed to the tilting plate 61, the support cylinder operates, causing the fixing top plate 733 on the fixing side plate 731 to move downwards along the stopper 72 of the culture flask 71 until the fixing port on the fixing top plate 733 is fitted onto the outside of the stopper 72. Then, the locking bolts 735 are rotated to fix the stopper 72, simultaneously disengaging the culture flask 71 from the tilting plate 61, causing the locking threads to disengage from the stopper 72. Finally, the support cylinder operates, disengaging the fixing top plate 733 from the piston.

[0064] The pusher 734 is disposed on the fixed side plate 731 and is used to drive the fixed top plate 733.

[0065] Please see Figures 10 to 14 The pusher 734 includes a guide plate 7341, a connecting pusher plate 7342, a drive shaft 7343, a rotating member 75, and a tensioning member 76. The guide plate 7341 is fixedly installed on the fixed side plate 731, and the guide plate 7341 is provided with a guide groove 77. In this application, the guide groove 77 is composed of a vertical end and an arc-shaped end, and one end of the arc-shaped end is connected to the vertical end. A first guide rod 78 and a second guide rod 79 slide through the guide groove 77. There are two connecting pushers 7342, which are located on both sides of the guide plate 7341 respectively. The two ends of the first guide rod 78 and the second guide rod 79 are fixedly connected to the two connecting pushers 7342.

[0066] Two connecting push plates 7342 are fixedly installed at the bottom of the fixed top plate 733. The drive shaft 7343 is rotatably connected to the fixed side plate 731. The rotating component 75 is used to drive the drive shaft 7343. In this embodiment, the rotating component 75 is preferably a rotating motor, that is, the rotating motor is fixedly installed on the fixed side plate 731. The rotating motor runs to further drive the drive shaft 7343. Two tensioning components 76 are provided. One end of each tensioning component 76 is connected to the drive shaft 7343, and the other end of the tensioning component 76 is connected to the connecting push plate 7342.

[0067] The tensioning member 76 includes a first rotating support rod 761 and a second rotating support rod 762. One end of the first rotating support rod 761 is fixedly sleeved on the outside of the drive shaft 7343, and the other end of the first rotating support rod 761 is rotatably connected to the second rotating support rod 762 through a pin. The other end of the second rotating support rod 762 is rotatably connected to the connecting push plate 7342 through a pin.

[0068] Specific implementation process: When the sealing cap 21 is opened, the cylinder is driven to operate, thereby causing the tilting plate 61 to rotate relative to the fixed frame 6. This causes the culture bottle 71 on the tilting plate 61 to rotate towards the pouring tube 2 until the mouth of the culture bottle 71 enters the flame range of the burning ring 41. At this time, the rotating motor is activated, causing the rotating shaft to rotate. As the rotating shaft rotates, the first rotating support rod 761 drives the second rotating support rod 762 to rotate. Due to the limiting action of the first guide rod 78 and the second guide rod 79, the connecting push plate 7342 moves upward relative to the guide plate 7341 and then rotates. The guide rod 78 and the second guide rod 79 first move along the vertical end until the second guide rod 79 moves to the arc end. Then, with the rotation limit of the first rotating support rod 761 and the second rotating support rod 762, the second guide rod 79 rotates along the arc end, while the first guide rod 78 remains stationary. This allows the stopper 72 to move and detach along the culture bottle 71. The stopper 72 then slowly unfolds at a certain angle along the culture bottle 71, entering an open state. At this time, the mouth of the culture bottle 71 just rotates to the open pouring tube 2. The Bacillus coagulans in the tilted culture bottle 71 then slowly and stably enters the interior of the culture tank body 1 through the pouring tube 2, enabling further efficient cultivation of microorganisms.

[0069] Example 2

[0070] Please see Figures 8 to 9 Example 2 further supplements the description of the fixing mechanism 74 in Example 1. Specifically, the fixing mechanism 74 includes a bidirectional threaded rod 741, a handle 742, a moving block 743, a fixing rod 744, and a fixing plate 745. The bidirectional threaded rod is rotatably connected to one side of the tilting plate 61, and the handle 742 is fixedly installed at one end of the bidirectional threaded rod 741. There are two moving blocks 743, which are respectively threaded onto the outside of the bidirectional threaded rod and slidably connected to the tilting plate 61. One end of the fixing rod 744 is fixedly connected to the moving block 743, and the other end of the fixing rod 744 is fixedly connected to the fixing plate 745. The two fixing plates 745 clamp and fix the culture bottle 71 to each other.

[0071] The specific implementation process is as follows: When fixing the culture bottle 71 containing Bacillus coagulans in the laboratory, first place the bottom of the culture bottle 71 on the annular groove 611, and then rotate the handle 742. While the handle 742 is rotating, the bidirectional threaded rod 741 is further rotated. While the bidirectional threaded rod 741 is rotating, it provides two moving blocks 743 with driving forces in opposite directions. Then, the two moving blocks 743 drive the two fixing plates 745 to move towards each other through the fixing rod 744 until the two fixing plates 745 clamp and fix the culture bottle 71, and then stop rotating the handle 742.

[0072] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0073] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A highly efficient microbial culture device, comprising a culture tank body (1), an inlet tube (2) disposed on the culture tank body (1), and a sealing cap (21) disposed on the inlet tube (2), characterized in that: The inlet tube (2) is provided with an inlet shell (3) on the outside, and the bottom of the inlet shell (3) is fixedly installed on the culture tank body (1); The inlet shell (3) is provided with an inlet (31), and the bottom of the inlet (31) is provided with a handle (4). One end of the handle (4) is connected to a fire ring (41), and the fire ring (41) is sleeved on the outside of the pouring tube (2). The top of the inlet shell (3) is provided with an opening mechanism (5), which is used to open the sealing cover (21); A fixed frame (6) is fixedly connected to one side of the bottom of the inlet shell (3). A tilting plate (61) is rotatably connected to the fixed frame (6). A microbial tilting mechanism (7) is provided on the tilting plate (61), and a driving component (8) for driving the tilting plate (61) is provided on the fixed frame (6). The microbial tilting mechanism (7) cooperates with the opening mechanism (5) to pour microorganisms from the pouring pipe (2) into the body of the culture tank (1).

2. The high-efficiency microbial culture device according to claim 1, characterized in that: The opening mechanism (5) includes a support rod (51), a movable shell (52), a clamping member (53), a telescopic connector (54), and a rotating member (55). The support rod (51) slides through the top of the inlet shell (3). The movable shell (52) is located inside the inlet shell (3) and is fixedly installed at the bottom of the support rod (51). The clamping member (53) is located inside the movable shell (52) and is used to clamp and fix the sealing cover (21). The telescopic connector (54) is located on the outer side of the top of the inlet shell (3), and the telescopic connector (54) is used to connect the support rod (51). The rotating part (55) is located on the outer side of the inlet shell (3), and the rotating part (55) is used to drive the support rod (51).

3. The high-efficiency microbial culture device according to claim 2, characterized in that: The clamping member (53) includes a heat insulation plate (531), an electric push rod (532), a push shell (533), a push bracket (534), a push support rod (535), and a contact member (536). The heat insulation plate (531) is fixedly installed inside the movable shell (52), and the electric push rod (532) is fixedly installed at the top inside the movable shell (52). The output end of the electric push rod (532) passes through the heat insulation plate (531) and is fixedly connected to the push shell (533), and the output end of the electric push rod (532) is slidably connected to the heat insulation plate (531). The push bracket (534) is provided in multiple ways. The multiple push brackets (534) are arranged around the bottom of the push shell (533). One end of the push support rod (535) is rotatably connected to the push bracket (534), and the other end of the push bracket (534) is connected to the contact member (536). The contact member (536) is slidably connected to the bottom of the movable shell (52), and the multiple contact members (536) lock and fix the sealing cover (21) together.

4. The high-efficiency microbial culture device according to claim 3, characterized in that: The contact element (536) includes a movable frame (5361) and a clamping plate (5362). The movable frame (5361) is slidably connected to the bottom of the movable shell (52), and one end of the push rod (535) is slidably connected to the movable frame (5361). The clamping plate (5362) is fixedly installed at the bottom of the movable frame (5361), and one end of the bottom of the clamping plate (5362) is the clamping end (5363).

5. The high-efficiency microbial culture device according to claim 1, characterized in that: The inlet shell (3) has an open opening (32) on one side corresponding to the microbial pouring mechanism (7).

6. The high-efficiency microbial culture device according to claim 5, characterized in that: The microbial pouring mechanism (7) includes a culture bottle (71), a stopper (72), a pull-out mechanism (73), and a fixing mechanism (74). The culture bottle (71) is set on the pouring plate (61), and the stopper (72) is set at the mouth of the culture bottle (71). The pull-out mechanism (73) is installed on the pouring plate (61), and when the culture bottle (71) is poured toward the pouring tube (2), the pull-out mechanism (73) slowly pulls out the stopper (72). The fixing mechanism (74) is installed on the pouring plate (61), and the fixing mechanism (74) is used to fix the culture bottle (71).

7. The high-efficiency microbial culture device according to claim 5, characterized in that: The tilting plate (61) is L-shaped and has an annular groove (611) for limiting the bottom of the culture bottle (71).

8. The high-efficiency microbial culture device according to claim 6, characterized in that: The pull-out mechanism (73) includes a fixed side plate (731), a support member (732), a fixed top plate (733), and a pusher member (734). The fixed side plate (731) is located on the outside of the top of the culture bottle (71). The support member (732) is installed on the tilting plate (61) and is used to support the fixed side plate (731). The fixed top plate (733) is provided with a fixing port and is fitted onto the outside of the bottle stopper (72) through the fixing port. The fixed top plate (733) is provided with two locking bolts (735) for locking the bottle stopper (72). The pusher (734) is disposed on the fixed side plate (731) and is used to drive the fixed top plate (733).

9. The high-efficiency microbial culture device according to claim 8, characterized in that: The pusher (734) includes a guide plate (7341), a connecting pusher plate (7342), a drive shaft (7343), a rotating member (75), and a tensioning member (76). The guide plate (7341) is fixedly installed on the fixed side plate (731), and the guide plate (7341) is provided with a guide groove (77). A first guide rod (78) and a second guide rod (79) slide through the guide groove (77). There are two connecting pushers (7342), and the two connecting pushers (7342) are located on both sides of the guide plate (7341). The two ends of the first guide rod (78) and the second guide rod (79) are fixedly connected to the two connecting pushers (7342). Two connecting push plates (7342) are fixedly installed at the bottom of the fixed top plate (733). The drive shaft (7343) is rotatably connected to the fixed side plate (731), and the rotating part (75) is used to drive the drive shaft (7343). Two tensioning parts (76) are provided. One end of each tensioning part (76) is connected to the drive shaft (7343), and the other end of the tensioning part (76) is connected to the connecting push plate (7342).

10. A high-efficiency microbial culture device according to claim 9, characterized in that: The tensioning member (76) includes a first rotating support rod (761) and a second rotating support rod (762). One end of the first rotating support rod (761) is fixedly sleeved on the outside of the drive shaft (7343), and the other end of the first rotating support rod (761) is rotatably connected to the second rotating support rod (762) through a pin. The other end of the second rotating support rod (762) is rotatably connected to the connecting push plate (7342) through a pin.