Brain tissue pathology sampling device

Abstract

The utility model discloses a brain tissue pathology sampling device which comprises a refrigeration groove with a first cavity and a second cavity, the first cavity is located below the second cavity, refrigerant is arranged in the first cavity, artificial cerebrospinal fluid is arranged in the second cavity, and a liquid inlet and a liquid outlet are formed in the side wall of the second cavity. The activity of tissue cells can be kept through the low-temperature environment and the artificial cerebrospinal fluid, and then the single cell sequencing quality is improved.

Description

Technical Field

[0001] The utility model relates to a pathological sampling device, in particular to a pathological sampling device for brain tissue. Background Art

[0002] At present, the pathological sampling of brain tissue is completed manually, and there are the following problems: 1. The fresh brain specimen is soft in texture, and the conventional manual cutting operation has a long cycle, which affects the integrity and activity of cells, and this is not conducive to subsequent single-cell sequencing. 2. The nerve cells in the brain tissue are very sensitive to the operating conditions, and the manual cutting process may lead to an increase in cell mortality, thus affecting the sequencing quality. Therefore, special measures are needed to ensure their activity. 3. Manual cutting often results in irregular edges, and it may not be possible to maintain the original spatial distribution and heterogeneity of cells in the tissue, which is very important for understanding the interaction and function between cells. Therefore, this application aims to provide a pathological sampling device for brain tissue that can overcome the above defects. Summary of the Utility Model

[0003] The purpose of the utility model is to overcome the shortcomings of the prior art and provide a pathological sampling device for brain tissue.

[0004] The purpose of the utility model is achieved by the following technical solutions: A pathological sampling device for brain tissue includes a refrigeration tank having a first cavity and a second cavity, the first cavity is located below the second cavity, a refrigerant is provided in the first cavity, artificial cerebrospinal fluid is provided in the second cavity, and an inlet and a drain are provided on the side wall of the second cavity.

[0005] Preferably, the pathological sampling device for brain tissue further includes a tissue cutting platform, the tissue cutting platform includes a first mounting plate and a plurality of cutting blades, the plurality of cutting blades are arranged at intervals parallel to each other on the first mounting plate, and a concave notch is provided on the cutting blade.

[0006] Preferably, the pathological sampling device for brain tissue further includes a slicing assembly, the slicing assembly includes a second mounting plate and a plurality of slices, the plurality of slices are arranged at intervals parallel to each other on the second mounting plate, and when the slicing assembly is inserted into the first cavity, the slices can be embedded in the gap formed by two cutting blades.

[0007] Preferably, a plurality of guide posts are provided on the refrigeration tank, a plurality of guide holes are provided on the second mounting plate, and the guide posts can be nested in the guide holes.

[0008] Preferably, a slice serial number is provided on each slice. Preferably, a storage box is detachably provided on the side of the refrigeration tank.

[0009] The utility model has the following advantages:

[0010] (1) The activity of tissue cells can be maintained through a low-temperature environment and artificial cerebrospinal fluid, thereby improving the quality of single-cell sequencing.

[0011] (2) In the prior art, pathological tissues are usually placed on a cutting platform for cutting, and the blade cuts the pathological tissues from top to bottom. After cutting, the pathological tissues need to be collected to make sections. The cutting platform of this application consists of cutting blades. After the section is pressed from top to bottom, the pathological tissue can directly remain between the two sections. Multiple cuts can be achieved with only one downward pressure during the cutting process, and the required cut edges are directly formed after cutting. The whole process is convenient and fast. Description of the Drawings

[0012] Figure 1 It is a schematic structural diagram of a brain tissue pathological sampling device in the front view state;

[0013] Figure 2 It is a schematic structural diagram of a brain tissue pathological sampling device in the top view state;

[0014] Figure 3 It is a schematic diagram of the setting method of the notch;

[0015] In the figure, 1 - refrigeration tank, 1a - first cavity, 1b - second cavity, 2 - liquid inlet, 3 - liquid outlet, 4 - tissue cutting platform, 4a - first mounting plate, 4b - cutting blade, 5 - notch, 6 - section assembly, 6a - second mounting plate, 6b - section, 7 - guide hole, 8 - guide post, 9 - accommodation box. Detailed Embodiments

[0016] The following further describes the present utility model with reference to the drawings. The protection scope of the present utility model is not limited to the following:

[0017] As Figures 1 to 3 shown, this application provides a brain tissue pathological sampling device, including a refrigeration tank 1 having a first cavity 1a and a second cavity 1b. The first cavity 1a is located below the second cavity 1b. A refrigerant is provided in the first cavity 1a, and artificial cerebrospinal fluid is provided in the second cavity 1b. Among them, a liquid inlet 2 and a liquid outlet 3 are provided on the side wall of the second cavity 1b. The refrigerant can be ice cubes, which can provide a low-temperature environment for the second cavity 1b. At the same time, the artificial cerebrospinal fluid can be continuously injected into the second cavity 1b through the liquid inlet 2 and the liquid outlet 3. The activity of tissue cells can be maintained through a low-temperature environment and artificial cerebrospinal fluid, thereby improving the quality of single-cell sequencing.

[0018] Preferably, the brain tissue pathological sampling device further includes a tissue cutting platform 4. The tissue cutting platform 4 includes a first mounting plate 4a and a plurality of cutting blades 4b. The plurality of cutting blades 4b are arranged at intervals parallel to each other on the first mounting plate 4a. Among them, a concave notch 5 is provided on the cutting blade 4b. A plurality of notches can form a large groove, so as to facilitate placing the pathological tissue to be cut. Specifically, the first mounting plate 4a can be integrally formed with the refrigeration tank 1. Plug holes can be provided on the first mounting plate 4a, and plug posts can be provided on the cutting blade 4b. The cutting blade can be detachably mounted on the first mounting plate by inserting the plug post into the plug hole, so that the section thickness can be adjusted by adjusting the distance between the cutting blades according to the sectioning requirements.

[0019] Preferably, the brain tissue pathological sampling device further includes a sectioning assembly 6. The sectioning assembly 6 includes a second mounting plate 6a and a plurality of sections 6b. The plurality of sections 6b are arranged at intervals parallel to each other on the second mounting plate 6a. Among them, when the sectioning assembly 6 is inserted into the first cavity 1a, the section 6b can be embedded in the gap formed by two cutting blades 4b. A plurality of sliding grooves can be provided on the second mounting plate 6a, and protrusions having the same shape as the sliding grooves can be provided at the ends of the sections. The sections can be detachably mounted on the second mounting plate by inserting the protrusions into the sliding grooves from the side. Similarly, the distance between the sections can also be adjusted correspondingly to meet the requirements of different section thicknesses. A plurality of guide posts 8 are provided on the refrigeration tank 1, and a plurality of guide holes 7 are provided on the second mounting plate 6a. The guide posts 8 can be nested in the guide holes 7. In actual use, after the pathological tissue to be cut is placed on the cutting blade, the sectioning assembly 6 is pressed down from top to bottom, and the cutting of the pathological tissue can be achieved. The cut pathological tissue can automatically remain between the two sections to form a required pathological section. In the prior art, the pathological tissue is usually placed on the cutting platform for cutting, and the blade cuts the pathological tissue from top to bottom. After cutting, the pathological tissue needs to be collected to make a section. The cutting platform of the present application is composed of cutting blades. After the section is pressed down from top to bottom, the pathological tissue can directly remain between the two sections. The cutting process only needs to be pressed down once to achieve multiple cuts, and at the same time, the required cut edge is directly formed after cutting. The whole process is convenient and fast.

[0020] Preferably, a section serial number is provided on each section 6b. The section serial number can be directly engraved on the side of the section by engraving. The position of each section tissue in the original pathological tissue can be quickly located through the section serial number. The section serial number can be composed of, for example, Arabic numerals "1, 2, 3", etc.

[0021] Preferably, a receiving box 9 is detachably arranged on the side of the refrigeration tank 1. For example, a T-shaped clamping groove can be arranged on the side of the refrigeration tank 1, and a T-shaped clamping protrusion is arranged on the side wall of the receiving box 9. The detachable connection between the two can be realized by embedding the clamping protrusion into the clamping groove. The receiving box is used to store artificial cerebrospinal fluid. It can be understood that a liquid discharge hole can be arranged on the side wall of the receiving box 9, and a driving device such as a small circulation pump can be arranged in the receiving box 9, so as to inject the artificial cerebrospinal fluid in the receiving box into the second cavity. The receiving box 9 and the liquid inlet 2 can be connected through a connecting hose.

[0022] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A device for collecting brain tissue pathology samples, characterized in that: The invention comprises a refrigeration tank (1) having a first cavity (1a) and a second cavity (1b), wherein the first cavity (1a) is located below the second cavity (1b), a refrigerant is arranged in the first cavity (1a), and artificial cerebrospinal fluid is arranged in the second cavity (1b), wherein a liquid inlet (2) and a liquid outlet (3) are arranged on the side wall of the second cavity (1b).

2. The brain tissue pathology sampling device according to claim 1, characterized in that: The brain tissue pathology sampling device also includes a tissue cutting platform (4), the tissue cutting platform (4) includes a first mounting plate (4a) and a plurality of cutting blades (4b), the plurality of cutting blades (4b) are arranged on the first mounting plate (4a) in parallel and at intervals, wherein the cutting blade (4b) is provided with a concave notch (5).

3. The device for obtaining brain tissue pathology samples according to claim 2, characterized in that: The brain tissue pathology sampling device also includes a slicing assembly (6), the slicing assembly (6) includes a second mounting plate (6a) and a plurality of slices (6b), the plurality of slices (6b) are arranged on the second mounting plate (6a) in parallel and spaced apart from each other, wherein when the slicing assembly (6) is inserted into the first cavity (1a), the slices (6b) can be embedded in the gap formed by the two cutting blades (4b).

4. The brain tissue pathology sampling device according to claim 3, characterized in that: A plurality of guide columns (8) are arranged on the refrigeration groove (1), and a plurality of guide holes (7) are arranged on the second mounting plate (6a), wherein the guide columns (8) can be nested in the guide holes (7).

5. The brain tissue pathology sampling device according to claim 3, characterized in that: Each of the slices (6b) is provided with a slice sequence number.

6. The device for obtaining brain tissue pathology samples according to claim 1, characterized in that: A detachable accommodation box (9) is provided on the side of the refrigeration tank (1).

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