Immunohistochemical staining instrument

Abstract

The immunohistochemical staining instrument comprises a workbench, a conveying device, a heating box and a sealing device, the conveying device, the heating box and the sealing device are all connected to the workbench, a reagent containing bin and a first opening are formed in the workbench, the heating box is arranged at the first opening and used for heating a glass slide, and the sealing device is arranged on the workbench. The conveying device is used for conveying a reagent in the reagent placing bin to the glass slide, the sealing device is used for sealing the first opening, the heating box is provided with a plurality of heating grooves corresponding to the first opening, and a heating element is embedded in each heating groove. In this way, each glass slide reacts with the staining reagent in a specific temperature environment, and the staining quality of the glass slides is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to an immunohistochemical staining instrument. Background Art

[0002] The immunohistochemical staining instrument is used in medical diagnosis and biological research to detect specific proteins or molecules in tissue sections or cell samples. This technique relies on the antigen-antibody reaction. By dropping specific reagents onto the glass slide and heating it, researchers and doctors can observe the target cells in the sample under a microscope. Currently, when the immunohistochemical staining instrument heats multiple glass slides, the heating device can only heat multiple glass slides at a fixed temperature simultaneously, unable to meet the specific temperature requirements of different samples, resulting in poor staining effects of the glass slides. Content of the Utility Model

[0003] The utility model provides an immunohistochemical staining instrument, which is used to solve the technical problem that the heating device can only heat multiple glass slides at a fixed temperature simultaneously, unable to meet the specific temperature requirements of different samples, resulting in poor staining effects of the glass slides.

[0004] The utility model is realized through the following technical solutions:

[0005] The utility model provides an immunohistochemical staining instrument, including: a workbench, a conveying device, a heating box and a sealing device, all of which are connected to the workbench. A reagent placement bin and a first opening are provided on the workbench. The heating box is arranged at the first opening and is used to heat the glass slide. The conveying device is used to convey the reagent in the reagent placement bin to the glass slide. The sealing device is used to seal the first opening. Among them, a plurality of heating grooves are provided corresponding to the first opening of the heating box, and a heating element is embedded in each heating groove.

[0006] Further, the sealing device includes: a support assembly, a fourth driving assembly connected to the support assembly, and a sealing plate connected to the fourth driving assembly. The fourth driving assembly drives the sealing plate to open or close, so as to open or seal the first opening.

[0007] Further, the support assembly includes: a first support plate provided with a lifting groove thereon; the fourth driving assembly includes: a second connecting member and a driving motor; the sealing plate is connected to the second connecting member, the driving motor is connected to the first support plate, the second connecting member is in transmission connection with the driving motor, the second connecting member is provided with a first guiding post and a second guiding post, both the first guiding post and the second guiding post are inserted into the lifting groove, and when the driving motor drives the second connecting member to rise or fall, both the first guiding post and the second guiding post slide in the lifting groove, so that the sealing plate opens or seals the first opening.

[0008] Further, the lifting groove includes: a first guiding groove, a second guiding groove and a guiding portion, the first guiding groove communicates with the second guiding groove, the guiding portion is arranged at the connection of the first guiding groove and the second guiding groove, when the driving motor drives the second connecting member to rise, the sealing plate is synchronously driven to rise, when the first guiding post abuts against the top end of the first guiding groove, the second guiding post abuts against the guiding portion, and when the driving motor continues to drive the second connecting member to rotate, the guiding portion guides the movement of the second guiding post, and synchronously drives the sealing plate to turn upwards, so as to open the first opening.

[0009] Further, the conveying device includes: a first driving assembly, a second driving assembly, a third driving assembly and a liquid transfer assembly; the first driving assembly is connected to the mobile end of the second driving assembly, the third driving assembly is connected to the mobile end of the second driving assembly, the liquid transfer assembly is connected to the third driving assembly, and the liquid transfer assembly is used for transferring the reagent in the reagent placement bin to the glass slide.

[0010] Further, the liquid transfer assembly includes: a fixing plate, a driving cylinder, a liquid transfer device and a first connecting member; the fixing plate is connected to the third driving assembly, the driving cylinder is connected to the fixing plate, the piston rod of the liquid transfer device is connected to the driving end of the driving cylinder, one end of the first connecting member is connected to the liquid transfer device, and the other end of the first connecting member is connected to the fixing plate.

[0011] Further, the immunohistochemical staining instrument further includes: a locking device, the heating box is connected to the workbench through the locking device, pushing the heating box, the heating box enters the workbench, and the locking device locks the heating box to the workbench; when the heating box is pushed again, the locking device is unlocked, and the heating box is separated from the workbench.

[0012] Further, the locking device includes a latch body and a locking hook. The latch body is fixedly connected to the workbench, and the locking hook is fixedly connected to the heating box.

[0013] Further, the latch body includes a fixed seat, an opening and closing jaw disposed in the fixed seat, a sliding member connected to the opening and closing jaw, a pull rod controlled by the sliding member to move, an elastic member abutted against the sliding member, and an upper cover. A sliding groove is provided on the fixed seat, a second opening is provided at the upper end of the sliding groove, the top of the sliding member is connected to the opening and closing jaw, one end of the elastic member abuts against the sliding member, and the other end abuts against the groove wall of the sliding groove. The upper end of the pull rod is swingably connected to the sliding member, and a protruding limit post is further provided at the upper end of the pull rod. The upper cover is connected to the fixed seat, a limit groove is provided on the upper cover, the limit post is inserted into the limit groove, and the limit groove includes a locking groove and an unlocking groove.

[0014] Further, the locking device further includes a sliding assembly. The sliding assembly includes a support frame connected to the workbench, a slide rail connected to the support frame, and a slider connected to the slide rail. The slider is connected to the side of the heating box.

[0015] Advantages of the present utility model:

[0016] Compared with the prior art, the immunohistochemical staining instrument proposed in this embodiment has multiple independent heating slots in the heating box, and each heating slot is embedded with a heating element, which can control the heating temperature of each glass slide, ensuring that each glass slide reacts with the staining reagent in a specific temperature environment, and improving the staining quality of the glass slide.

[0017] The above description is only an overview of the technical solution of the present utility model. In order to be able to understand the technical means of the present utility model more clearly, it can be implemented according to the content of the specification. And in order to make the above and other purposes, features and advantages of the present utility model more obvious and understandable, the following preferred embodiments are specifically described in detail as follows. Description of the Drawings

[0018] Figure 1 It is a schematic diagram of the overall structure of an immunohistochemical staining instrument of the present utility model;

[0019] Figure 2 It is a schematic diagram of the internal structure of an immunohistochemical staining instrument of the present utility model;

[0020] Figure 3 It is a schematic diagram of the overall structure of the pipetting assembly in an immunohistochemical staining instrument of the present utility model;

[0021] Figure 4Schematic diagram of the overall structure of the sealing device in an immunohistochemical staining instrument of the present utility model;

[0022] Figure 5 Schematic diagram of the side view structure of the sealing device in an immunohistochemical staining instrument of the present utility model;

[0023] Figure 6 Schematic diagram of the connection relationship structure between the locking device and the heating box in an immunohistochemical staining instrument of the present utility model;

[0024] Figure 7 Schematic diagram of the sliding component structure in an immunohistochemical staining instrument of the present utility model;

[0025] Figure 8 Front view structure schematic diagram of the lock body and the lock hook in the locking device of an immunohistochemical staining instrument of the present utility model;

[0026] Figure 9 Internal structure schematic diagram of the lock body and the lock hook in the locking device of an immunohistochemical staining instrument of the present utility model;

[0027] Figure 10 Workflow diagram of an immunohistochemical staining instrument of the present utility model.

[0028] Explanation of reference numerals:

[0029] 1. Workbench; 11. Reagent placement bin; 111. Reagent placement grid; 12. First storage bin; 13. Second storage bin; 2. Transport device; 21. First drive assembly; 22. Second drive assembly; 23. Third drive assembly; 24. Pipetting assembly; 241. Fixed plate; 242. Driving cylinder; 243. Liquid transfer device; 244. First connecting piece; 3. Heating box; 31. Heating groove; 4. Sealing device; 41. Support assembly; 411. First support plate; 412. Lifting groove; 4121. First guiding groove; 4122. Second guiding groove; 4123. Guiding part; 413. Second support plate; 42. Fourth drive assembly; 421. Second connecting piece; 422. First guiding column; 423. Second guiding column; 424. Connecting rod; 425. Third connecting piece; 426. Fourth connecting piece; 427. Fifth connecting piece; 428. Sixth connecting piece; 429. Driving motor; 43. Sealing plate; 5. Locking device; 51. Lock body; 511. Fixed seat; 5111. Side plate; 5112. Sliding groove; 5113. Second opening; 512. Opening and closing jaws; 513. Sliding part; 514. Elastic part; 515. Pull rod; 5151. Limit post; 516. Upper cover; 5161. Limit groove; 51611. Locking groove; 51612. Unlocking groove; 51613. Limiting part; 52. Lock hook; 53. Sliding component; 531. Support frame; 532. Slide rail; 533. Slide block; 6. Console. Detailed implementation manners

[0030] In order to make the objectives, technical solutions and advantages of the present utility model clearer and more understandable, the present utility model will be further described in detail below with reference to the accompanying drawings and specific implementation manners.

[0031] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without making creative efforts belong to the scope of protection of the present utility model.

[0032] In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.

[0033] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, "a plurality" means two or more unless otherwise specifically defined.

[0034] In the present utility model, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected to", "fixed", etc. should be understood in a broad sense. For example, it may be a connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the internal connection of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0035] In the present utility model, unless otherwise clearly defined and limited, the first feature being "above" or "below" the second feature may include the direct contact between the first and second features, or may include the situation where the first and second features are not in direct contact but in contact through additional features therebetween. Moreover, the first feature being "above", "over" and "on top of" the second feature includes that the first feature is directly above and obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "below", "beneath" and "under" the second feature includes that the first feature is directly below and obliquely below the second feature, or merely indicates that the horizontal height of the first feature is lower than that of the second feature.

[0036] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples", etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic expressions of the above terms should not be understood as necessarily referring to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine the different embodiments or examples described in this specification.

[0037] Refer to Figures 1 to 9 , an immunohistochemical staining instrument is proposed in an embodiment of the present utility model, which includes: a workbench 1, a conveying device 2, a heating box 3 and a sealing device 4 that are all connected to the workbench 1. A reagent placement bin 11 and a first opening (not marked in the figure) are provided on the workbench 1. The heating box 3 is arranged at the first opening. The heating box 3 is used to heat the glass slide. The conveying device 2 is used to convey the reagent in the reagent placement bin 11 to the glass slide. The sealing device 4 is used to seal the first opening. Among them, a plurality of heating grooves 31 are provided in the heating box 3, and a heating element (not marked in the figure) is embedded in each heating groove 31.

[0038] It should be specifically noted that a control console 6 is installed on the workbench 1, and the heating element (not marked in the figure), the sealing device 4 and the conveying device 2 are all controlled by the control console 6.

[0039] In this embodiment, a first storage bin 12 and a second storage bin 13 are provided on the workbench 1. The first storage bin 12 is used to store the cleaning agent, and the second storage bin 13 is used to store the waste liquid generated after cleaning the liquid transfer device 243 in the cleaning pipetting assembly 24. A plurality of reagent placement grids 111 are provided in the reagent placement bin 11.

[0040] In this embodiment, the heating element is embedded in the bottom of the heating tank 31, so that the heat is conducted upward from the bottom of the heating tank 31, so that the bottom of the slide is heated first, and then the temperature in the entire heating tank 31 is evenly increased, avoiding local overheating or uneven heating.

[0041] In this embodiment, the heating element may be any one of a resistance wire, a ceramic heating plate, a PTC heater, and an electric heating coil.

[0042] In one embodiment, heating elements are embedded in the bottom and side walls of the heating tank 31, so that heat is transferred to the slide in the heating tank 31 from multiple directions at the same time, reducing the temperature gradient, making the sample heated more evenly, and avoiding local overheating or cooling.

[0043] To apply the technical solution of this embodiment, first, the operator places the glass slide in the heating tank 31, and the transport device 2 transports the staining reagent from the reagent placement chamber 11 to the glass slide; secondly, the sealing device 4 seals the first opening (not shown in the figure) to prevent the reagent from evaporating; finally, according to the chemical reaction characteristics of the staining reagent and the glass slide, the operating console 6 sets the heating temperature of the corresponding heating tank 31 to heat the glass slide.

[0044] The immunohistochemical staining instrument proposed in this embodiment has a plurality of independent heating slots 31 in the heating box 3. Each heating slot 31 is embedded with a heating element, which can control the heating temperature of each glass slide, ensuring that each glass slide reacts with the staining reagent under a specific temperature environment, thereby improving the staining quality of the glass slide.

[0045] See also Figure 4 and Figure 5 The sealing device 4 includes: a supporting component 41, a fourth driving component 42 connected to the supporting component 41 and a sealing plate 43 connected to the fourth driving component 42, and the fourth driving component 42 drives the sealing plate 43 to open or close, so as to open or seal the first opening.

[0046] Please refer again Figure 4 and Figure 5 The support assembly 41 includes: a first support plate 411, and a lifting slot 412 is provided on the first support plate 411; the fourth driving assembly 42 includes: a second connecting member 421 and a driving motor 429; the sealing plate 43 is connected to the second connecting member 421, and the driving motor 429 is connected to the first support plate 411. The second connecting member 421 is transmission-connected with the driving motor 429, and the second connecting member 421 is provided with a first guide column 422 and a second guide column 423. The first guide column 422 and the second guide column 423 are both inserted in the lifting slot 412. When the driving motor 429 drives the second connecting member 421 to rise or fall, the first guide column 422 and the second guide column 423 both slide in the lifting slot 412, so that the sealing plate 43 opens or seals the first opening.

[0047] In this embodiment, the fourth driving assembly 42 further includes: a connecting rod 424, a fourth connecting member 426, a fifth connecting member 427, and a sixth connecting member 428. One end of the connecting rod 424 is connected to the second connecting member 421, and the other end of the connecting rod 424 is connected to the fourth connecting member 426. One end of the fifth connecting member 427 is connected to the fourth connecting member 426, and the other end of the fifth connecting member 427 is connected to the output end of the driving motor 429. The driving motor 429 is connected to the first support plate 411 through the sixth connecting member 428. During use, the driving motor 429 drives the fourth connecting member 426 to rotate, causing the second connecting member 421 to rise or fall, and synchronously driving the first guide post 422 and the second guide post 423 to slide in the lifting groove 412, so that the sealing plate 43 opens or seals the first opening.

[0048] In another embodiment, the support assembly 41 further includes: a second support plate 413. The first support plate 411 and the second support plate 413 are oppositely arranged and have the same structure. The fourth driving assembly 42 further includes: a third connecting member 425. The third connecting member 425 is oppositely arranged to the second connecting member 421 and has the same structure. The second connecting member 421 is connected to one side of the sealing plate 43, and the third connecting member 425 is connected to the other side of the sealing plate 43. One end of the connecting rod 424 is connected to the second connecting member 421, and the other end of the connecting rod 424 is connected to the third connecting member 425. One end of the fourth connecting member 426 is connected to the rod body of the connecting rod 424. The first guide post 422 and the second guide post 423 are also provided on the third connecting member 425. The first guide post 422 and the second guide post 423 provided on the third connecting member 425 are evenly inserted into the lifting groove 412 of the second support plate 413. During use, the driving motor 429 drives the fourth connecting member 426 to rotate, causing the second connecting member 421 and the third connecting member 425 to rise or fall, and synchronously driving the first guide post 422 and the second guide post 423 to slide in the lifting groove 412, so that the sealing plate 43 opens or seals the first opening. By connecting the second connecting member 421 and the third connecting member 425 to both sides of the sealing plate 43 respectively, when the sealing plate 43 opens or seals the first opening, the movement of the sealing plate 43 is stable.

[0049] Please refer to again Figure 4 and Figure 5The lifting groove 412 includes: a first guide groove 4121, a second guide groove 4122 and a guide portion 4123. The first guide groove 4121 is connected to the second guide groove 4122. The guide portion 4123 is arranged at the connection between the first guide groove 4121 and the second guide groove 4122. When the driving motor 429 drives the second connecting member 421 to rise, the sealing plate 43 is driven to rise synchronously. When the first guide column 422 abuts against the top end of the first guide groove 4121, the second guide column 423 abuts against the guide portion 4123. When the driving motor 429 continues to drive the second connecting member 421 to rotate, the guide portion 4123 moves and guides the second guide column 423, and synchronously drives the sealing plate 43 to flip upward, so as to open the first opening. When sealing the first opening, the driving motor 429 drives the second connecting member 421 to rotate, and synchronously drives the sealing plate 43 to flip downward. When the second guide column 423 abuts against the guide part 4123, when the driving motor 429 continues to drive the second connecting member 421 to descend, the guide part 4123 moves and guides the second guide column 423, and synchronously drives the sealing plate 43 to descend, thereby sealing the first opening.

[0050] See also Figure 2 The transport device 2 includes: a first drive component 21, a second drive component 22, a third drive component 23 and a pipetting component 24; the first drive component 21 is connected to the moving end of the second drive component 22, the third drive component 23 is connected to the moving end of the second drive component 22, the pipetting component 24 is connected to the third drive component 23, and the pipetting component 24 is used to transfer the reagent in the reagent placement chamber 11 to the slide.

[0051] Specifically, the third driving component 23 drives the pipetting component 24 to move in the Z direction, so that the pipetting component 24 absorbs the dye from the reagent placement compartment 11 or delivers the absorbed dye to the slide. The first driving component 21 drives the pipetting component 24 to move in the X direction, so that the pipetting component 24 reciprocates between the heating box 3 and the position where the reagent placement compartment 11 is located. The second driving component 22 drives the pipetting component 24 to move in the Y direction, and moves the pipetting component 24 to the position where the reagent placement grid 111 or the heating tank 31 is located in the Y direction, and the pipetting component 24 absorbs the dye in the reagent placement grid 111 or delivers the absorbed dye to the slide in the heating tank 31.

[0052] It should be particularly noted that the first drive assembly 21 , the second drive assembly 22 and the third drive assembly 23 have the same structure, and all include: a motor and a linear module connected to the motor.

[0053] See also Figure 3, the pipetting assembly 24 includes: a fixing plate 241, a driving cylinder 242, a liquid transfer device 243 and a first connecting member 244; the fixing plate 241 is connected to the third driving assembly 23, the driving cylinder 242 is connected to the fixing plate 241, the piston rod of the liquid transfer device 243 is connected to the driving end of the driving cylinder 242, one end of the first connecting member 244 is connected to the liquid transfer device 243, and the other end of the first connecting member 244 is connected to the fixing plate 241.

[0054] Specifically, when the driving end of the driving cylinder 242 moves upward, the piston rod of the liquid transfer device 243 moves upward synchronously, sucking the staining reagent in the reagent placement grid 111 into the liquid transfer device 243; conversely, when the driving end of the driving cylinder 242 moves downward, the staining reagent is pushed into the liquid transfer device 243.

[0055] Please refer to Figure 2 and Figure 7 , the immunohistochemical staining instrument further includes: a locking device 5, the heating box 3 is connected to the workbench 1 through the locking device 5, pushing the heating box 3, the heating box 3 enters into the workbench 1, and the locking device 5 locks the heating box 3 to the workbench 1; when the heating box 3 is pushed again, the locking device 5 is unlocked, and the heating box 3 is separated from the workbench 1.

[0056] Please refer to Figure 6 , the locking device 5 includes: a lock body 51 and a lock hook 52, the lock body 51 is fixedly connected to the workbench 1, and the lock hook 52 is fixedly connected to the heating box 3.

[0057] Please refer to Figures 8 to 9, the buckle body 51 includes: a fixed seat 511, an opening and closing jaw 512 disposed within the fixed seat 511, a sliding member 513 connected to the opening and closing jaw 512, a pull rod 515 controlled to move by the sliding member 513, an elastic member 514 abutting against the sliding member 513, and an upper cover 516; the opening and closing jaw 512 is provided with a position corresponding to the locking hook 52, and when the opening and closing jaw 512 is closed, the locking hook 52 is locked in the position. The fixed seat 511 is provided with a sliding groove 5112, and the sliding groove 5112 is enclosed by side plates 5111 vertically bent upward from a pair of opposite sides of the fixed seat 511. The upper end of the sliding groove 5112 is provided with a second opening 5113 for the opening and closing jaw 512 to extend or retract. The top of the sliding member 513 is connected to the opening and closing jaw 512, one end of the elastic member 514 abuts against the sliding member 513, and the other end abuts against the groove wall of the sliding groove 5112. The acting direction of the elastic member 514 on the sliding member 513 is the same as the telescopic direction of the opening and closing jaw 512. The upper end of the pull rod 515 is swingably connected to the sliding member 513, and a protruding limit post 5151 is further provided at the upper end of the pull rod 515. The upper cover 516 is connected to the fixed seat 511, and a limit groove 5161 is provided on the upper cover 516. The limit post 5151 is inserted into the limit groove 5161, and the limit groove 5161 is used to lock different strokes of the limit post 5151, synchronously realizing the switching of the opening, closing, extending, and retracting states of the clamping mouth of the opening and closing jaw 512.

[0058] Please refer to Figure 8 , the limit groove 5161 includes a locking groove 51611 and an unlocking groove 51612. The locking groove 51611 is in communication with the unlocking groove 51612, and a limit portion 51613 is provided at the junction of the locking groove 51611 and the unlocking groove 51612. When the limit post 5151 is located in the unlocking groove 51612, the opening and closing jaw 512 extends out of the sliding groove 5112, and the clamping mouth of the opening and closing jaw 512 is in an open state. At this time, the spring is in a natural state or a slightly compressed state, and at the same time, the heating box 3 is located outside the workbench 1; when the locking hook 52 is inserted into the clamping mouth of the opening and closing jaw 512 and simultaneously drives the opening and closing jaw 512 to move downward along the sliding groove 5112, the limit post 5151 moves downward along the guiding of the unlocking groove 51612. Under the action of the limit portion 51613, the limit post 5151 enters the locking groove 51611. At this time, the locking device 5 is in a locked state, and at the same time, the heating box 3 enters the workbench 1. Conversely, when the heating box 3 is pushed again, the limit post 5151 exits the locking groove 51611 and enters the unlocking groove 51612, realizing the separation of the heating box 3 from the workbench 1.

[0059] Please refer to Figure 7, the locking device 5 further includes: a sliding assembly 53; the sliding assembly 53 includes: a support frame 531 connected to the workbench 1, a slide rail 532 connected to the support frame 531, and a slider 533 connected to the slide rail 532, and the slider 533 is connected to the side of the heating box 3.

[0060] Specifically, when performing immunohistochemical reaction on the glass slide, first place the glass slide in the heating tank 31, and then the operator pushes the heating box 3 along the slide rail 532. When the heating box 3 reaches the designated position. At this time, the locking device 5 locks the heating box 3 to the workbench 1; when the immunohistochemical reaction of the glass slide is completed, the operator pushes the heating box 3 again to make the locking device 5 in the unlocked state, and then through the sliding of the slider 533 on the slide rail 532, the heating box 3 smoothly moves from the inside of the workbench 1 to the outside.

[0061] Please refer to Figure 10 , the following briefly describes the working process of the immunohistochemical staining instrument proposed in this embodiment:

[0062] Step S1, the operator pushes the heating box 3 to move it from inside the workbench 1 to the outside;

[0063] Step S2, the operator places the glass slide in the heating tank 31 inside the heating box 3, and then pushes the heating box 3 to make it enter the workbench 1;

[0064] Step S3, the operator operates the console 6 to make the conveying device 2 convey the staining reagent to the side of the first opening;

[0065] Step S4, the operator operates the console 6 to make the sealing device 4 open the first opening;

[0066] Step S5, the operator operates the console 6 to make the conveying device 2 convey the stain to the corresponding glass slide;

[0067] Step S6, the operator operates the console 6 to make the sealing device 4 seal the first opening;

[0068] Step S7, the operator sets the heating temperature of the corresponding heating tank 31 according to the chemical reaction characteristics of the staining reagent and the glass slide, and heats the glass slide.

[0069] Compared with the prior art, in the immunohistochemical staining instrument proposed in this embodiment, a plurality of independent heating tanks 31 are provided in the heating box 3, and each heating tank 31 is embedded with a heating element, which can control the heating temperature of each glass slide, ensure that each glass slide reacts with the staining reagent in a specific temperature environment, and improve the staining quality of the glass slide.

[0070] The above are only specific embodiments of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model can easily think of various equivalent modifications or substitutions, and these modifications or substitutions should all be covered within the protection scope of the present utility model. Therefore, the protection scope of the present utility model shall be subject to the protection scope of the claims.

Claims

1. An immunohistochemical staining instrument, characterized in that: include: A workbench and a conveying device, a heating box and a sealing device all connected to the workbench, wherein the workbench is provided with a reagent placement chamber and a first opening, the heating box is provided at the first opening, the heating box is used to heat the glass slide, the conveying device is used to convey the reagent in the reagent placement chamber to the glass slide, and the sealing device is used to seal the first opening, wherein the heating box is provided with a plurality of heating grooves corresponding to the first opening, and each of the heating grooves is embedded with a heating element.

2. The immunohistochemical staining instrument according to claim 1, characterized in that: The sealing device includes: a supporting assembly, a fourth driving assembly connected to the supporting assembly, and a sealing plate connected to the fourth driving assembly. The fourth driving assembly drives the sealing plate to open or close, so as to open or seal the first opening.

3. The immunohistochemical staining instrument according to claim 2, characterized in that: The support assembly includes: a first support plate, on which a lifting slot is provided; the fourth drive assembly includes: a second connecting member and a drive motor; the sealing plate is connected to the second connecting member, the drive motor is connected to the first support plate, the second connecting member is transmission-connected to the drive motor, the second connecting member is provided with a first guide column and a second guide column, the first guide column and the second guide column are both inserted in the lifting slot, when the drive motor drives the second connecting member to rise or fall, the first guide column and the second guide column both slide in the lifting slot, so that the sealing plate opens or seals the first opening.

4. The immunohistochemical staining instrument according to claim 3, characterized in that: The lifting groove includes: a first guide groove, a second guide groove and a guide portion, the first guide groove is connected to the second guide groove, the guide portion is arranged at the connection between the first guide groove and the second guide groove, when the driving motor drives the second connecting member to rise, the sealing plate is synchronously driven to rise, when the first guide column abuts against the top end of the first guide groove, the second guide column abuts against the guide portion, when the driving motor continues to drive the second connecting member to rotate, the guide portion moves and guides the second guide column, synchronously drives the sealing plate to flip upward, so as to open the first opening.

5. The immunohistochemical staining instrument according to claim 1, characterized in that: The transport device includes: a first drive component, a second drive component, a third drive component and a pipetting component; the first drive component is connected to the moving end of the second drive component, the third drive component is connected to the moving end of the second drive component, the pipetting component is connected to the third drive component, and the pipetting component is used to transfer the reagent in the reagent placement chamber to the glass slide.

6. The immunohistochemical staining instrument according to claim 5, characterized in that: The pipetting assembly includes: a fixed plate, a driving cylinder, a liquid transferor and a first connecting member; the fixed plate is connected to the third driving assembly, the driving cylinder is connected to the fixed plate, the piston rod of the liquid transferor is connected to the driving end of the driving cylinder, one end of the first connecting member is connected to the liquid transferor, and the other end of the first connecting member is connected to the fixed plate.

7. The immunohistochemical staining instrument according to claim 1, characterized in that: The immunohistochemical staining instrument also includes: a locking device, through which the heating box is connected to the workbench. When the heating box is pushed, the heating box enters the workbench, and the locking device locks the heating box to the workbench; when the heating box is pushed again, the locking device is unlocked, and the heating box is separated from the workbench.

8. The immunohistochemical staining instrument according to claim 7, characterized in that: The locking device comprises: a lock body and a lock hook, wherein the lock body is fixedly connected to the workbench, and the lock hook is fixedly connected to the heating box.

9. The immunohistochemical staining instrument according to claim 8, characterized in that: The lock body includes: a fixed seat, an opening and closing jaw arranged in the fixed seat, a sliding piece connected to the opening and closing jaw, a pull rod controlled by the movement of the sliding piece, an elastic piece abutting against the sliding piece, and an upper cover; the fixed seat is provided with a sliding groove, the upper end of the sliding groove is provided with a second opening, the top of the sliding piece is connected to the opening and closing jaw, one end of the elastic piece abuts against the sliding piece, and the other end abuts against the groove wall of the sliding groove, the upper end of the pull rod is swingably connected to the sliding piece, and the upper end of the pull rod is also provided with a raised limiting column; the upper cover is connected to the fixed seat, the upper cover is provided with a limiting groove, the limiting column is inserted in the limiting groove, and the limiting groove includes a locking groove and an unlocking groove.

10. The immunohistochemical staining instrument according to claim 8, characterized in that: The locking device also includes: a sliding assembly; the sliding assembly includes: a support frame connected to the workbench, a slide rail connected to the support frame and a slider connected to the slide rail, and the slider is connected to the side of the heating box.

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