Sampling and detecting device for pathogenic bacteria

Abstract

The utility model discloses a pathogenic bacterium sampling and detecting device which comprises a sterile protective cover, a support frame, a sterile diluent storage tank, a culture dish, a liquid guide pipe and a liquid conveying pipe, a supporting frame is installed in the sterile protection cover, a sterile diluent storage tank is arranged on the supporting frame, a sealing cover is arranged at an opening in the bottom of the sterile diluent storage tank, a puncture outfit is arranged in the center of the sealing cover, a liquid guide pipe is fixed to the bottom of the puncture outfit, and the bottom end of the liquid guide pipe is connected with three infusion pipes through a three-way pipe. Drip cups are respectively arranged on the three infusion tubes; the bottom of the drip cup is connected with the culture dish. The device is provided with a sterile protection cover, is convenient to carry, can be used in a general operation area and a quasi-clean area, and is easy to operate, visual in identification, low in cost and high in universality; a prefabricated identification culture medium is arranged in the culture dish, and a result can be quickly obtained after sampling is completed, so that the sampling time and the sample circulation time are saved, and the working efficiency is improved.

Description

Technical Field

[0001] The utility model relates to a pathogenic bacteria sampling and detection device, belonging to the technical field of food detection. Background Art

[0002] Pathogenic bacteria are microorganisms that can cause diseases, also known as pathogenic microorganisms, which include bacteria, viruses, spirochetes, rickettsiae, chlamydiae, mycoplasmas, fungi, actinomycetes, etc. Foodborne diseases caused by foodborne pathogenic bacteria have become one of the most concerned public health problems in the world today. In recent years, due to various reasons, there are various unsafe factors in food, and the number of food safety problems has increased rapidly, and food safety accidents occur frequently. The relevant national government departments attach great importance to food safety issues.

[0003] During the processing and production of food, due to factors such as unqualified sanitation or improper operation, it is easy to cause the risk of pathogenic bacteria contamination. To ensure food safety, it is necessary to detect pathogenic bacteria in food. After retrieval, Chinese patent CN217521119U discloses an instant food pathogenic bacteria rapid detection device, which drives the rotation of the rotating shaft through a motor, and the rotating shaft drives the Escherichia coli detector to rotate synchronously. The detection of multiple foods can be completed by rotating the rotating shaft one week. However, when detecting, the food is exposed to the air and is easily affected by the external environment. Therefore, a new type of pathogenic bacteria detection device is proposed. Summary of the Utility Model

[0004] Aiming at the deficiency that the food is exposed to the air and is easily affected by the external environment during food detection in the prior art, the utility model provides a pathogenic bacteria sampling and detection device; it is mainly used in the environment outside the laboratory, saving the time for transporting the sample back to the laboratory for detection.

[0005] The technical solution adopted by the utility model is as follows:

[0006] A pathogenic bacteria sampling and detection device includes a sterile protective cover, a support frame, a sterile diluent storage tank, a puncture device, a three-way pipe, a stop flow clamp, a drip kettle, a sterile protective cap, a culture dish, a sealing cover, a liquid guide pipe, a sealing door, a handle, a support leg, an infusion pipe;

[0007] The support frame is fixedly installed inside the sterile protective cover, and the sterile diluent storage tank is arranged on the support frame. A sealing cover is arranged at the bottom opening of the sterile diluent storage tank, and a puncture device is arranged at the center of the sealing cover. A liquid guide pipe is fixedly installed at the bottom of the puncture device, and a three-way pipe is arranged at the bottom end of the liquid guide pipe. The three-way pipe is respectively connected to three infusion pipes, and drip kettles are respectively arranged on the three infusion pipes; the bottom of the drip kettle is connected to the culture dish.

[0008] Preferably, three groups of the infusion tubes are arranged in parallel in the vertical direction, and a stop clamp and a drip chamber are successively provided on each infusion tube, and the number of the stop clamps and the drip chambers are both three groups.

[0009] Preferably, the support frame and the sterile diluent storage tank are matched. The opening of the sterile diluent storage tank faces downward, and it is placed upside down and just stuck on the support ring of the support frame. During use, inserting the puncture device into the sealing cover belongs to normal medical means.

[0010] Preferably, the culture dish is provided with a sterile protective cap, and the number of the sterile protective caps and the culture dishes are both three groups. During sampling, the culture dish is connected to the infusion tube; before and after sampling, the culture dish is sealed with the sterile protective cap. Specifically, before sampling, there is a pre-prepared identification culture medium in the culture dish, and the culture dish cover is sealed with the sterile protective cap; during sampling and testing, the sterile protective cap is removed, and the culture dish is connected to the infusion tube; after sampling is completed, the culture dish is removed, the sterile protective cap is put on the culture dish to seal it, and wait for the test result.

[0011] Preferably, a sealing door is provided on the front surface of the sterile protective cover, and a lockable doorknob is provided on the front surface of the sealing door.

[0012] Preferably, a handle is fixedly installed on the top surface of the sterile protective cover, and an anti-slip sleeve is provided on the surface of the handle.

[0013] Preferably, support legs are fixedly installed on the bottom surface of the sterile protective cover, and the number of the support legs is four groups.

[0014] The sterile protective cover and the support frame are fixedly connected, and the two are connected by threading or welding.

[0015] The using process of the pathogenic bacteria sampling and detecting device of the present utility model is as follows: by fixedly placing the sterile diluent storage tank on the support frame, inserting the puncture device into the sealing cover, adjusting the stop clamp, under the action of gravity, the liquid in the sterile diluent storage tank flows into the drip chamber, connecting the drip chamber outlet to the culture dish, connecting all the culture dishes in sequence, and squeezing the drip chamber to make the internal liquid flow into the culture dish, and observing the test result.

[0016] The pathogenic bacteria detecting device provided by the present utility model brings the following beneficial effects:

[0017] (1) The device is provided with a sterile protective cover, which is convenient to carry, and can be used in general working areas and semi-clean areas, is easy to operate, has intuitive identification, low cost, and strong universality.

[0018] (2) There is a pre-prepared identification culture medium in the culture dish, and the result can be obtained quickly after sampling is completed, saving the sampling time and the sample transfer time, and improving the work efficiency.

[0019] The present utility model will be further described below in conjunction with the accompanying drawings and embodiments. Description of the Drawings

[0020] Figure 1 It is the external structure diagram of the present utility model;

[0021] Figure 2 It is the schematic diagram of the internal structure of the present utility model;

[0022] Figure 3 It is the schematic diagram of the structure of the support frame of the present utility model;

[0023] Figure 4 It is the internal structure diagram of the present utility model;

[0024] Figure 5 It is the schematic diagram of the structure of the culture dish of the present utility model.

[0025] In the figure: 1, aseptic protective cover; 2, support frame; 3, aseptic diluent storage tank; 4, puncture device; 5, three-way pipe; 6, flow stop clamp; 7, drip kettle; 8, aseptic protective cap; 9, culture dish; 10, sealing cover; 11, liquid guide pipe; 12, sealing door; 13, handle; 14, support leg; 15, infusion tube. Detailed Embodiment Embodiment

[0026] As Figures 1 to 5 shown, the present utility model provides a pathogenic bacteria sampling and detection device, including an aseptic protective cover 1, a support frame 2, an aseptic diluent storage tank 3, a puncture device 4, a three-way pipe 5, a flow stop clamp 6, a drip kettle 7, an aseptic protective cap 8, a culture dish 9, a sealing cover 10, a liquid guide pipe 11, a sealing door 12, a handle 13, a support leg 14, and an infusion tube 15;

[0027] A support frame 2 is fixedly installed inside the aseptic protective cover 1, an aseptic diluent storage tank 3 is arranged on the support frame 2, a sealing cover 10 is arranged at the bottom opening of the aseptic diluent storage tank 3, a puncture device 4 is arranged at the center of the sealing cover 10, a liquid guide pipe 11 is fixedly installed at the bottom of the puncture device 4, a three-way pipe 5 is arranged at the bottom end of the liquid guide pipe 11, the three-way pipe is respectively connected to three infusion tubes 15, and drip kettles 7 are respectively arranged on the three infusion tubes; the bottom of the drip kettle is connected to the culture dish 9.

[0028] Preferably, three groups of the infusion tubes 15 are arranged in parallel in the vertical direction, a flow stop clamp 6 and a drip kettle 7 are sequentially arranged on each infusion tube, and the number of the flow stop clamps and the drip kettles is three groups. Through the arrangement of the flow stop clamp 6, the liquid flow rate in the infusion tube 15 can be adjusted, and excessive liquid outflow can be avoided, which affects the detection result.

[0029] Preferably, the support frame 2 is matched with the sterile diluent storage tank 3. The opening of the sterile diluent storage tank faces downward, and when it is placed upside down on the support ring of the support frame, it is just stuck. When in use, inserting the puncture device into the sealing cover belongs to normal medical means.

[0030] Preferably, the culture dish 9 is provided with a sterile protective cap 8, and the number of both the sterile protective cap and the culture dish is three groups. Through the setting of the sterile protective cap 8, the culture dish 9 can be sealed to prevent external environmental pollution of the culture dish 9. When sampling, the culture dish 9 is connected to the infusion tube 15; before and after sampling, the culture dish 9 is sealed with the sterile protective cap 8. Specifically, before sampling, there is a pre-prepared identification medium in the culture dish, and the culture dish cover is sealed with the sterile protective cap; when sampling and detecting, the sterile protective cap is removed, and the culture dish is connected to the infusion tube; after sampling is completed, the culture dish is removed, and the sterile protective cap is put on the culture dish to seal it, waiting for the test results.

[0031] Preferably, a sealing door 12 is provided on the front surface of the sterile protective cover 1, and a lockable doorknob is provided on the front surface of the sealing door. Through the setting of the sealing door 12, the sterile protective cover 1 is sealed, reducing the entry of bacteria in the environment into the interior of the sterile protective cover 1.

[0032] Preferably, a handle 13 is fixedly installed on the top surface of the sterile protective cover, and an anti-slip sleeve is provided on the surface of the handle. The setting of the handle 13 facilitates the tester to move this device.

[0033] Preferably, support legs 14 are fixedly installed on the bottom surface of the sterile protective cover, and the number of the support legs is four groups. The setting of the support legs 14 provides support for this device, preventing the bottom surface of the sterile protective cover 1 from directly contacting the ground or the tabletop and reducing the wear of the bottom surface of the sterile protective cover 1.

[0034] Preferably, the sterile protective cover and the support frame are fixedly connected, and the two are connected by threading or welding.

[0035] When in use: Inject ethylene oxide into the interior of the sterile protective cover 1 for sterilization. After sterilization is completed, first weigh the test sample and mix it in the sterile diluent in the sterile diluent storage tank 3. Open the sealing door 12, fixedly place the sterile diluent storage tank 3 on the support frame 2, insert the puncture device 4 into the sealing cover 10, adjust the stop clamp 6, and under the action of gravity, the liquid in the sterile diluent storage tank 3 flows into the drip chamber 7. Remove the sterile protective cap 8 on the culture dish 9, connect the outlet of the drip chamber 7 to the culture dish 9, connect all the culture dishes 9 in sequence, and squeeze the drip chamber 7 to make the internal liquid flow into the culture dish 9 for culturing and observing the results. This utility model is provided with a sterile protective cover 1, which is convenient to carry and use in different cleanliness areas, saves sampling time and sample transfer time, is easy to operate, has intuitive identification, low cost, and strong universality.

[0036] Compared with the existing rapid pathogen detection devices, when the utility model is in use, first weigh 25 g of the test sample, place it in 225 mL of sterile diluent and mix evenly, and then let the diluent flow into multiple pre-prepared pathogen identification culture dishes by its own weight (auxiliary pressure), 1 mL for each dish, spread evenly for cultivation, and observe the results. It can also be used as a disposable product, avoiding cleaning and maintenance.

[0037] It should be noted that in this text, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

[0038] Although the embodiments of the present utility model have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A pathogenic bacteria sampling and detection device, characterized in that: Including sterile protective cover, support frame, sterile diluent storage tank, puncture device, three-way tube, stop clamp, drip pot, sterile protective cap, culture dish, sealing cover, catheter, sealing door, handle, support leg, infusion tube; A support frame is fixedly installed inside the sterile protective cover, a sterile diluent storage tank is arranged on the support frame, a sealing cover is arranged at the bottom opening of the sterile diluent storage tank, a puncture device is arranged at the center of the sealing cover, a catheter is fixedly installed at the bottom of the puncture device, a three-way pipe is arranged at the bottom end of the catheter, the three-way pipe is respectively connected to three infusion tubes, and drip pots are respectively arranged on the three infusion tubes; the bottom of the drip pot is connected to the culture dish.

2. The pathogenic bacteria sampling and detection device according to claim 1, characterized in that: The infusion tubes are arranged in three groups in parallel in the vertical direction, and each infusion tube is provided with a flow stop clamp and a drip pot in sequence, and the number of the flow stop clamps and the drip pot is three groups.

3. The pathogenic bacteria sampling and detection device according to claim 1, characterized in that: The support frame matches the sterile diluent storage tank, the opening of the sterile diluent storage tank faces downward, and the tank is placed upside down on the support ring of the support frame and is just stuck.

4. The pathogenic bacteria sampling and detection device according to claim 1, characterized in that: The culture dish is equipped with a sterile protective cap, and the number of the sterile protective cap and the culture dish is three sets; when sampling, the culture dish is connected to the infusion tube; before and after sampling, the culture dish is sealed with the sterile protective cap.

5. The pathogenic bacteria sampling and detection device according to claim 1, characterized in that: A sealed door is arranged on the front of the sterile protective cover, and a locked door handle is arranged on the front of the sealed door.

6. The pathogenic bacteria sampling and detection device according to claim 1, characterized in that: A handle is fixedly mounted on the top surface of the sterile protective cover, and an anti-slip cover is arranged on the surface of the handle.

7. The pathogenic bacteria sampling and detection device according to claim 1, characterized in that: The bottom surface of the sterile protective cover is fixedly mounted with supporting legs, and the number of the supporting legs is four groups.

8. The pathogenic bacteria sampling and detection device according to claim 1, characterized in that: The sterile protective cover and the support frame are connected by threads or welding.

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