Simple condensation element titer detection device
By using technical means such as reverse osmosis membrane and scale marks in the cold agglutinin titer detection device, the fusion problem between the NaCL solution layer and the red blood cell layer and the inaccurate measurement of solution volume are solved, and the accuracy and accuracy of the detection results are achieved.
Patent Information
- Application Number
- CN202422016174.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-20
- Publication Date
- 2025-06-27
- Estimated Expiration
- 2034-08-20
AI Technical Summary
When used in the existing cold agglutinin titer detection device, the NaCL solution layer is prone to fusing with pre-packed ‘O’ red blood cells, resulting in distortion of the result and it is difficult to accurately measure the amount of solution entering the next level, resulting in inaccurate detection results.
A simple cold agglutinin titer detection device was designed to separate the prefabricated ‘O’ red cell layer from the NaCL solution layer through the reverse osmosis membrane to prevent the damage to the red cell by the NaCL solution layer, and accurately measure and confirm it through the scale and check the test results to ensure the accuracy of the detection results.
It effectively avoids rupture or deformation of the cell membrane of red blood cells, improves the accuracy of the detection results, and ensures accurate measurement and confirmation of solution content.
Smart Images
Figure CN223037957U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of medical treatment, in particular to a simple cold agglutinin titer detection device. Background Art
[0002] The normal reference value of cold agglutinin titer is less than 1:16 (4°C). This reference value applies to the IgM cold agglutinin against red blood cell antigens in the normal human serum layer. In medicine, the determination of cold agglutinin titer is of great significance for the diagnosis of certain diseases. For example, in cases such as mycoplasma pneumonia, infectious mononucleosis, malaria, cirrhosis, lymphoma, and multiple myeloma, the cold agglutinin titer may increase, but usually does not exceed 1:1000. In addition, the results of the cold agglutinin test can also be used to monitor the activity and treatment effect of diseases. Cold agglutinin is an antibody that easily binds to red blood cell membrane antigens under low-temperature conditions (such as 0°C to 5°C), belonging to the IgM type, and has a strong ability to agglutinate red blood cells. Although a small amount of cold agglutinin is contained in the normal human serum layer and the low titer generally has no clinical significance, in some pathological conditions, such as mycoplasma pneumonia and cirrhosis, there may be high-titer cold agglutinin, which may lead to the distortion of test items. The most common influence is the abnormal results of blood cell analysis, difficult blood type identification, and cross-matching.
[0003] When the existing technical solutions are used,
[0004] (1) During the process of pressing down the piston, the NaCl solution layer is liable to fuse with the pre-coated "O" type red blood cells, which is likely to affect the results;
[0005] (2) It is difficult to simply measure the amount of solution entering the next stage, which is likely to lead to inaccurate test results.
[0006] In view of the above problems, the utility model provides a simple cold agglutinin titer detection device. Content of the Utility Model
[0007] The purpose of the utility model is to provide a simple cold agglutinin titer detection device. The utility model can separate the pre-coated "O" type red blood cell layer from the NaCl solution layer through a reverse osmosis membrane, can block the NaCl solution layer, avoid damaging the cell membrane of red blood cells, resulting in cell rupture or deformation, etc., and can accurately confirm the content of the NaCl solution layer and the serum layer entering the next stage through scale lines, and accurately measure the content of the solution through the remaining residue inside the device body, thereby solving the problems in the background art.
[0008] To achieve the above object, the present utility model provides the following technical solution: A simple cold agglutinin titer detection device, including a device body, wherein the inner side wall of the device body is fixedly connected with dextran gel, the top of the dextran gel is fixedly connected with a pre-coated "O" type red blood cell layer, the top of the pre-coated "O" type red blood cell layer is fixedly connected with a reverse osmosis membrane, one side of the device body is fixedly connected with a scale line, the other side of the device body is fixedly connected with a connecting pipe, and a one-way valve assembly is fixedly connected inside the connecting pipe.
[0009] Further, the one-way valve assembly includes a one-way valve fixedly connected to the inner side wall of the connecting pipe, and a valve is rotatably connected to the top of the one-way valve, achieving a control effect.
[0010] Further, a piston is slidably connected to the inner side wall of the device body, and a push rod is fixedly connected to the top of the piston, achieving a pushing effect.
[0011] Further, a 200UL 0.95% NaCl solution layer is fixedly connected to the top of the reverse osmosis membrane, and a 200UL serum layer is fixedly connected to the top of the 200ul 0.95% NaCl solution layer, achieving a detection effect.
[0012] Further, a liquid inlet is fixedly connected to the front surface of the device body, and a dust-proof and leak-proof membrane is fixedly connected to the inner side wall of the liquid inlet, achieving a liquid inlet effect.
[0013] Further, a stabilizing block is fixedly connected to the bottom of the device body, and an anti-slip pad is fixedly connected to the top of the stabilizing block, achieving an anti-slip effect.
[0014] Compared with the prior art, the beneficial effects of the present utility model are as follows:
[0015] A simple cold agglutinin titer detection device provided by the present utility model
[0016] (1) Through the setting of the reverse osmosis membrane and the pre-coated "O" type red blood cell layer, a separation effect is achieved during use. The reverse osmosis membrane can separate the pre-coated "O" type red blood cell layer from the NaCl solution layer, blocking the NaCl solution layer and avoiding damage to the cell membrane of red blood cells, resulting in cell rupture or deformation, etc.
[0017] (2) Through the setting of the scale line and the one-way valve assembly, a metering effect is achieved during use. The scale line can accurately confirm the content of the NaCl solution layer and the serum layer entering the next stage, and the remaining residue inside the device body is used to accurately measure the content of the solution. The one-way valve can prevent the solution from flowing back, improving the accuracy of the detection result. Description of the Drawings
[0018] Figure 1 Schematic diagram of the device body of the present utility model;
[0019] Figure 2 Schematic diagram of the scale line of the present utility model;
[0020] Figure 3 Schematic diagram of the pre-coated "O" type red blood cell layer of the present utility model;
[0021] Figure 4 Schematic diagram of the liquid inlet of the present utility model.
[0022] In the figure: 1. Device body; 2. Dextran gel; 3. Pre-coated "O" type red blood cell layer; 4. Reverse osmosis membrane; 5. Scale line; 6. Connecting pipe; 7. Check valve assembly; 701. Check valve; 702. Valve; 8. Piston; 9. Push rod; 10. NaCL solution layer; 11. Serum layer; 12. Liquid inlet; 13. Dust-proof and leak-proof membrane; 14. Stabilizing block; 15. Anti-slip pad. Specific embodiments
[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.
[0024] To solve the technical problem of how to effectively position and adjust, as Figures 1-4 shown, the following preferred technical solutions are provided:
[0025] A simple cold agglutinin titer detection device includes a device body 1. The inner side wall of the device body 1 is fixedly connected with a dextran gel 2. Through the setting of the dextran gel 2, it plays a role in separating and purifying substances during detection, thereby achieving the detection effect. The top of the dextran gel 2 is fixedly connected with a pre-coated "O"-type red blood cell layer 3. Through the setting of the pre-coated "O"-type red blood cell layer 3, it can avoid interference caused by antigen-antibody reactions and can more accurately reflect the interaction between the substance studied in the experiment and red blood cells, thereby achieving the protection effect. The top of the pre-coated "O"-type red blood cell layer 3 is fixedly connected with a reverse osmosis membrane 4. Through the setting of the reverse osmosis membrane 4, the pre-coated "O"-type red blood cell layer 3 can be separated from the NaCl solution layer 10 through the reverse osmosis membrane 4, and the NaCl solution layer 10 can be blocked to avoid damaging the cell membrane of red blood cells, resulting in cell rupture or deformation, etc., thereby achieving the effect of smooth detection. One side of the device body 1 is fixedly connected with a scale line 5, and the other side of the device body 1 is fixedly connected with a connecting pipe 6. A one-way valve assembly 7 is fixedly connected inside the connecting pipe 6. Through the settings of the scale line 5 and the one-way valve assembly 7, the content of the NaCl solution layer 10 and the serum layer 11 entering the next stage can be accurately confirmed through the scale line 5, and the content of the solution can be accurately measured through the remaining residue inside the device body 1. The one-way valve 701 can prevent the solution from flowing back, improving the accuracy of the detection result.
[0026] Specifically, the reverse osmosis membrane 4 at the top of the pre-coated "O"-type red blood cell layer 3 can separate the pre-coated "O"-type red blood cell layer 3 from the NaCl solution layer 10 through the reverse osmosis membrane 4, and the NaCl solution layer 10 can be blocked to avoid damaging the cell membrane of red blood cells, resulting in cell rupture or deformation, etc.
[0027] Further, as Figure 2 shown, the following preferred technical solutions are provided:
[0028] The one-way valve assembly 7 includes a one-way valve 701 fixedly connected to the inner side wall of the connecting pipe 6. The top of the one-way valve 701 is rotatably connected with a valve 702. The purpose of this design is to control the one-way valve 701 through the valve 702, thereby achieving the control effect.
[0029] Further, as Figure 2 shown, the following preferred technical solutions are provided:
[0030] The inner side wall of the device body 1 is slidably connected with a piston 8. The top of the piston 8 is fixedly connected with a push rod 9. The purpose of this design is to control the piston 8 through the push rod 9, thereby achieving the control effect.
[0031] Further, as Figure 3As shown in the figure, the following preferred technical solutions are provided:
[0032] At the top of the reverse osmosis membrane 4, a 200 UL 0.95% NaCl solution layer 10 is fixedly connected. At the top of the 200 ul 0.95% NaCl solution layer 10, a 200 UL serum layer 11 is fixedly connected. The purpose of this design is to maintain the osmotic pressure balance and stable ionic strength of the solution through the NaCl solution layer 10, thus achieving a stable effect.
[0033] Furthermore, as Figure 2 shown in the figure, the following preferred technical solutions are provided:
[0034] A liquid inlet 12 is fixedly connected to the front of the device body 1. A dust-proof and leak-proof membrane 13 is fixedly connected to the inner side wall of the liquid inlet 12. The purpose of this design is to protect the liquid inlet 12 of the device body 1 and the internal solution through the dust-proof and leak-proof membrane 13, thus achieving the effect of dust-proof and leak-proof.
[0035] Furthermore, as Figure 1 shown in the figure, the following preferred technical solutions are provided:
[0036] A stabilizing block 14 is fixedly connected to the bottom of the device body 1. An anti-slip pad 15 is fixedly connected to the top of the stabilizing block 14. The purpose of this design is to protect and fix the device body 1 through the stabilizing block 14, preventing the device body 1 from tilting, etc., thus achieving the protective effect.
[0037] In summary:
[0038] 1. Inside the device body 1, a 200 UL 0.95% NaCl solution layer 10 is pre-embedded. Medical staff can add a 200 UL serum layer 11 into the inside of the device body 1 from the liquid inlet 12. A dust-proof and leak-proof membrane 13 is fixedly connected between the liquid inlet 12 and the inside of the device body 1, which can protect the liquid inlet 12, etc.;
[0039] 2. The reverse osmosis membrane 4 at the top of the pre-coated "O" type red blood cell layer 3 can separate the pre-coated "O" type red blood cell layer 3 from the NaCl solution layer 10 through the reverse osmosis membrane 4, and can block the NaCl solution layer 10 to prevent the destruction of the red blood cell membrane, resulting in cell rupture or deformation, etc.;
[0040] 3. By pushing the piston 8 with the push rod 9, under the action of the piston 8, the solution will enter the inside of the next-stage device body 1 through the connecting pipe 6. A one-way valve 701 is fixedly connected to the inside of the connecting pipe 6, which can prevent the reverse flow of the solution.
[0041] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.
[0042] Although embodiments of the present utility model have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.
Claims
1. A simple cold agglutinin titer detection device, comprising a device body (1), characterized in that: The inner wall of the device body (1) is fixedly connected to a dextran gel (2), the top of the dextran gel (2) is fixedly connected to a prefabricated "O" type red blood cell layer (3), the top of the prefabricated "O" type red blood cell layer (3) is fixedly connected to a reverse osmosis membrane (4), one side of the device body (1) is fixedly connected to a scale line (5), the other side of the device body (1) is fixedly connected to a connecting tube (6), and the inside of the connecting tube (6) is fixedly connected to a one-way valve assembly (7).
2. A simple cold agglutinin titer detection device according to claim 1, characterized in that: The one-way valve assembly (7) comprises a one-way valve (701) fixedly connected to the inner wall of the connecting pipe (6), and a valve (702) is rotatably connected to the top of the one-way valve (701).
3. A simple cold agglutinin titer detection device according to claim 1, characterized in that: The inner side wall of the device body (1) is slidably connected to a piston (8), and the top of the piston (8) is fixedly connected to a push rod (9).
4. A simple cold agglutinin titer detection device according to claim 1, characterized in that: A 200 UL 0.95% NaCL solution layer (10) is fixedly connected to the top of the reverse osmosis membrane (4), and a 200 UL serum layer (11) is fixedly connected to the top of the 200 ul 0.95% NaCL solution layer (10).
5. A simple cold agglutinin titer detection device according to claim 1, characterized in that: A liquid inlet (12) is fixedly connected to the front of the device body (1), and a dust and leak-proof membrane (13) is fixedly connected to the inner wall of the liquid inlet (12).
6. A simple cold agglutinin titer detection device according to claim 1, characterized in that: A stabilizing block (14) is fixedly connected to the bottom of the device body (1), and an anti-slip pad (15) is fixedly connected to the top of the stabilizing block (14).