Suction head and storage box for wound healing experiment

Abstract

The utility model relates to the technical field of bioengineering, in particular to a suction head and a storage box for a wound healing experiment (also called scratch experiment), the suction head comprises a tube body and a suction cup structure, the tube body is of a hollow columnar structure with a thick upper part and a thin lower part, and the suction cup is fixed at the bottom of the tube body. The suction head for the wound healing experiment can generate a uniform circular cell-free area, and is suitable for the wound healing experiment. Compared with two common modes in the current scratch experiment, the suction head of the device can generate a circular cell-free area with the diameter of 1mm or 1.5 mm, and the process does not cause mechanical damage to cells to influence the cell migration effect, so that the device is more in line with the movement mode of the cells, and is convenient for microscopic observation of cell migration conditions and statistics; the experiment operation is more convenient and efficient; the suction head is placed in the sterile box, so that pollution is avoided to the greatest extent, and use and mass production are facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of bioengineering, in particular to a pipette tip and a storage box for wound healing experiments. Background Art

[0002] The wound healing assay, also known as the cell scratch assay, is a standard in vitro assay method for analyzing two-dimensional cell collective migration. Its operation and monitoring are simple, and it allows the study of cell behavior under strictly controlled conditions. Cell migration is a key process involved in many biological processes, including embryonic development, tissue formation, wound healing, immune defense or inflammation, and cancer progression. In this process, cells must be able to change in a specific environment and migrate to a suitable position to perform their functions. The principle of the wound healing assay is to create a cell-free area in a confluent monolayer of cells by physical barrier or by mechanical, thermal, or chemical damage. The exposure of the cell-free area induces cells to migrate into the gap.

[0003] There are mainly two traditional methods. One is that when the cell density reaches 100% and a monolayer of cells is formed, a sterile pipette tip is used to create a scratch on the cell surface. However, it is difficult to ensure the consistency of the scratch width and the straightness of the scratch with this method. There are large differences between groups, poor repeatability, and it is easy to cause cell contamination. When observing under the microscope after the experiment, it is not easy to locate. When conducting experiments on some culture well plates that require special coating, the coating on the surface of the well plate may be scratched, thus affecting the experimental results. The other is to use a culture insert. The insert is expensive and not reusable, and its usage frequency in scientific research is low. The insert is small in volume. During operation, due to the separate sample addition treatment in the partition compartments, it is easy to cause inconsistent results. These two methods have their own advantages and disadvantages. The common point is that both finally produce vertical or horizontal rectangular blank areas. However, according to the latest research, the movement pattern of population cells under circular geometric constraints is spiral.

[0004] Therefore, the utility model provides a pipette tip and a storage box for wound healing experiments, which are simple and fast to operate. On the premise of not damaging cells and the culture well plate, a circular cell-free area with uniform size is formed. Only the suction cup at the tail of the pipette tip contacts the cells, maximizing the avoidance of cell contamination. The circular cell-free area is more in line with the movement pattern of cells, and the cell migration situation can be better observed under the microscope. Moreover, the price is controllable, which is conducive to scientific research promotion and use. Summary of the Utility Model

[0005] Aiming at the above deficiencies in the prior art, the utility model provides a pipette tip and a storage box for wound healing experiments, with a practical structure and convenient for promotion.

[0006] A pipette tip for wound healing experiments, comprising a tube body and a suction cup. The tube body is a hollow columnar structure that is thicker at the top and thinner at the bottom, and the suction cup is fixed to the bottom of the tube body.

[0007] Preferably, the suction cup is made of biocompatible silica gel that is non-toxic to cells, and the tube body is made of a plastic that is resistant to high temperature and high pressure.

[0008] Preferably, the diameter of the circular area generated by the suction cup is 1 mm or 1.5 mm, the length of the tube body is 49 mm, and the top diameter of the tube body is 6.5 mm.

[0009] A storage box, comprising a box body and a box cover. A plurality of placement holes are provided in the box body for placing pipette tips for wound healing experiments.

[0010] The beneficial effects of the present utility model are as follows:

[0011] The pipette tip for wound healing experiments of the present utility model can generate a uniform circular cell-free area and is suitable for wound healing experiments. Compared with the two common methods currently used in wound healing experiments, the pipette tip of this device can generate a circular cell-free area with a diameter of 1 mm or 1.5 mm. This process will not cause mechanical damage to the cells and affect the cell migration effect, which is more in line with the movement pattern of the cells, facilitating microscopic observation of cell migration and statistics; the experimental operation is more convenient and efficient; the pipette tips are placed in a sterile box, minimizing contamination and facilitating use and mass production. Description of the Drawings

[0012] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the following will briefly introduce the drawings required for use in the embodiments. It should be understood that the following drawings only show certain embodiments of the present utility model and should not be regarded as limiting the scope. For those of ordinary skill in the art, other related drawings can be obtained based on these drawings without creative efforts.

[0013] Figure 1 It is a perspective view of a pipette tip for wound healing experiments;

[0014] Figure 2 It is a front view of a pipette tip for wound healing experiments;

[0015] Figure 3 It is a perspective view of a storage box;

[0016] 1 - tube body, 2 - suction cup, 3 - placement hole, 4 - box body, 5 - box cover. Detailed Embodiments

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model. Components of the embodiments of the present utility model described and illustrated in the accompanying drawings here can be arranged and designed in various different configurations.

[0018] In the description of the embodiments of the present utility model, it should be noted that if terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are used to indicate the orientation or positional relationship, it is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of this utility model is usually placed when in use. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model. In addition, terms such as "first", "second", "third", etc. are only used for distinguishing descriptions and cannot be understood as indicating or implying relative importance.

[0019] In addition, if terms such as "horizontal", "vertical", "hanging" are used, it does not mean that the component is required to be absolutely horizontal or hanging, but can be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0020] As shown in the accompanying drawings, a pipette tip for wound healing experiments includes a tube body 1 and a suction cup 2. The tube body 1 is a hollow columnar structure that is thicker at the top and thinner at the bottom. The suction cup 2 is fixed to the bottom of the tube body 1. The diameter of the suction cup 2 is 1 mm or 1.5 mm, the length of the tube body 1 is 49 mm, and the diameter of the top of the tube body 1 is 6.5 mm.

[0021] The tube body 1 is made of a plastic that is resistant to high temperature and high pressure, and the suction cup 2 is made of a biocompatible silicone that is non-toxic to cells.

[0022] A storage box includes a box body 4 and a box cover 5. A number of placement holes 3 are provided in the box body 4 for placing pipette tips for wound healing experiments. The storage box is sterile and free of RNase and DNase. After the pipette tips are placed in the storage box, the height of the box cover exceeds the top of the pipette tips, and the bottom of the pipette tips does not contact the bottom of the storage box.

[0023] Before use, place the whole box of pipette tips for wound healing experiments in the ultra-clean bench for ultraviolet irradiation. When using, align the conical part of the pipettor with the nozzle of the pipette tip for wound healing experiments, gently press down vertically with force to make it fit tightly, lift out the pipette tip, move it above the cell layer, press down appropriately to make the suction cup fully adhere to the surface of the cell layer for several seconds, and then lift it vertically. A circular cell-free area with a diameter of 1 mm or 1.5 mm can be seen on the cell layer, indicating that the operation is completed. Press the pipettor ejection button firmly down to the end to safely remove the pipette tip. During experimental statistics, the migration ability of cells is reflected by the area change of the circular area. (To ensure the experimental effect, the cell culture medium needs to be aspirated before the operation, and the phosphate buffer solution is used to rinse after the operation to remove the fallen cells)

[0024] The above are only the preferred embodiments of the utility model patent, and are not intended to limit the utility model patent. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the utility model patent shall be included within the protection scope of the utility model patent.

Claims

1. A suction tip for wound healing experiments, characterized in that: It comprises a tube body (1) and a suction cup (2); the tube body (1) is a hollow columnar structure that is thick at the top and thin at the bottom; and the suction cup (2) is fixed to the bottom of the tube body (1).

2. A wound healing experiment suction tip according to claim 1, characterized in that: The suction cup (2) is made of biocompatible silicone that is non-toxic to cells, and the tube body (1) is made of a plastic material that is resistant to high temperature and high pressure.

3. A wound healing experiment suction tip according to claim 1, characterized in that: The diameter of the circular area produced by the suction cup (2) is 1 mm or 1.5 mm; the length of the tube body (1) is 49 mm, and the top diameter of the tube body (1) is 6.5 mm.

4. A storage box, characterized in that: The invention comprises a box body (4) and a box cover (5), wherein the box body (4) is provided with a plurality of placement holes (3) for placing the suction tip for wound healing experiment as claimed in any one of claims 1 to 3.

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