Collection and detection device for oligomer in macroporous adsorption resin

Abstract

The utility model relates to the technical field of detection devices, and discloses a device for collecting and detecting oligomers in macroporous adsorption resin, which comprises a flask, a peristaltic pump, a chromatographic column and a microporous filter, the flask is used for containing water; the other end of the peristaltic pump is connected with a water inlet at the bottom end of the chromatographic column, and a water outlet at the top end of the chromatographic column is communicated with the microporous filter; the bottom end of the microporous filter is communicated with the flask; the chromatographic column is used for washing resin, and a filter membrane for intercepting particles is arranged in the microporous filter. According to the utility model, water is conveyed into the chromatographic column through the peristaltic pump for washing, particles washed out of the chromatographic column are intercepted through the filter membrane, the mass of the particles can be obtained by comparing the mass of the dried filter membrane with the mass of the filter membrane before filtration, and the content of the particles in resin per unit mass can be calculated.

Description

Technical Field

[0001] The utility model relates to the technical field of detection devices, and particularly relates to a device for collecting and detecting oligomers in macroporous adsorption resins. Background Art

[0002] There are a large number of micropores in the resin for hemoperfusion, and a large number of substances with low degree of polymerization exist in the micropores. The substances with low degree of polymerization will continuously precipitate in the micropores. These substances with low degree of polymerization have a risk of sensitization during the clinical process and may endanger the lives of patients. There is a lack of a device for detecting the content of such substances with low degree of polymerization in the resin in the prior art. Summary of the Utility Model

[0003] Aiming at the problems existing in the prior art, the purpose of the utility model is to provide a device for collecting and detecting oligomers in macroporous adsorption resins, so as to solve the problems that there is a lack of a device for detecting the content of substances with low degree of polymerization in the resin in the prior art, and the substances with low degree of polymerization have a risk of sensitization during the clinical process.

[0004] A device for collecting and detecting oligomers in macroporous adsorption resins includes a flask, a peristaltic pump, a chromatography column and a microporous filter.

[0005] The flask is used for holding water; one end of the peristaltic pump is communicated with the flask, and the other end is connected to the water inlet at the bottom of the chromatography column. The water outlet at the top of the chromatography column is communicated with the microporous filter; the bottom of the microporous filter is communicated with the flask; the chromatography column is used for flushing the resin, and a filter membrane for intercepting microparticles is arranged in the microporous filter.

[0006] Further, the detection device further includes a constant temperature water bath, which is used for heating the water in the flask.

[0007] Further, the microporous filter includes a first funnel, a second funnel, a filter membrane, a dust-proof cover and a fixing clip.

[0008] A dust-proof cover is arranged at the top of the first funnel, and the dust-proof cover is connected to the water outlet at the top of the chromatography column; the bottom of the first funnel and the top of the second funnel clamp the filter membrane; the fixing clip is used for connecting the first funnel and the second funnel; the bottom end of the second funnel is inserted into the flask.

[0009] Further, the flask is a round-bottom flask.

[0010] Further, a first port is arranged at the top of the flask, and the bottom end of the second funnel is inserted into the first port.

[0011] Further, a rubber stopper is sleeved at the bottom end of the second funnel; the outer diameter of the rubber stopper is adapted to the inner diameter of the first port.

[0012] Further, second ports are provided on both sides of the flask. One second port is for one end of the peristaltic pump to extend into the flask, and the other second port is provided with a plug.

[0013] Beneficial effects: In the present utility model, water is transported into the chromatography column through the peristaltic pump for flushing, and the particles flushed out of the chromatography column are intercepted by the filter membrane. By comparing the mass of the filter membrane after drying with the mass of the filter membrane before filtration, the mass of the particles can be obtained, and the content of particles in the resin per unit mass can be calculated. Resin with a low particle content is selected, thereby reducing the risk of sensitization. Description of the Drawings

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model, the drawings required to be used in the specification will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings.

[0015] Figure 1 It is a schematic diagram of the overall structure of the embodiment of the present utility model.

[0016] In the figure: 1, constant temperature water bath; 2, peristaltic pump; 3, valve; 4, chromatography column; 5, microporous filter; 501, first funnel; 502, second funnel; 503, dust cover; 504, fixing clip; 6, rubber stopper; 7, flask; 8, plug. Detailed Embodiments

[0017] The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the drawings in the embodiments of the present utility model. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model. For ease of description, the terms "vertical", "horizontal", "left", "right", "up", "down", "inside", "outside", "bottom", etc. used in this specification indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for facilitating the description of the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation of the present application.

[0018] It should be noted that the embodiments and the features involved in the embodiments of the present utility model can be combined with each other without conflict. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present utility model.

[0019] Such as Figure 1A device for collecting and detecting oligomers in macroporous adsorption resin shown in the figure, including a constant temperature water bath 1, a flask 7, a peristaltic pump 2, a chromatography column 4 and a microporous filter 5;

[0020] The flask 7 is used to hold water; one end of the peristaltic pump 2 is connected to the flask 7, and the other end is connected to the water inlet at the bottom of the chromatography column 4. The peristaltic pump 2 is used to transfer the water in the flask 7 into the chromatography column 4 and wash the resin arranged in the chromatography column 4 with water; the water outlet at the top of the chromatography column 4 is connected to the microporous filter 5. A filter membrane is detachably installed in the microporous filter 5, and the filter membrane is used to intercept oligomers; the bottom of the microporous filter 5 is connected to the flask 7. The water contained in the flask 7 is purified water, and the purified water is prior art.

[0021] In this embodiment, the peristaltic pump 2 is started, and the peristaltic pump 2 moves the water in the flask 7 into the chromatography column 4 to wash the resin located in the chromatography column 4, flushes the fine particles in the resin out of the chromatography column 4 and drives the fine particles into the microporous filter 5 by water. The water in the microporous filter 5 moves into the flask 7 under the action of gravity; the water circulates and moves in the flask 7, the peristaltic pump 2, the chromatography column 4 and the microporous filter 5. After repeatedly washing the resin in the chromatography column 4 a certain number of times, the peristaltic pump 2 is shut down, the filter membrane in the microporous filter 5 is removed, and the filter membrane is dried. The mass of the dried filter membrane is calculated with the mass of the filter membrane before filtration, and the mass of the washed-out oligomers can be obtained.

[0022] A valve 3 is arranged at one end of the peristaltic pump 2 close to the washing column.

[0023] In this embodiment, the valve 3 is used to control the flow rate of the water input into the chromatography column 4.

[0024] The microporous filter 5 includes a first funnel 501, a second funnel 502, a dust-proof cover 503, a fixing clip 504 and a filter membrane; a dust-proof cover 503 is arranged at the top end of the first funnel 501, and the dust-proof cover 503 is connected to the chromatography column 4; the filter membrane is arranged between the first funnel 501 and the second funnel 502, and the bottom of the first funnel 501 and the top of the second funnel 502 clamp the filter membrane; the fixing clip 504 is used to connect the first funnel 501 and the second funnel 502; the bottom end of the second funnel 502 is inserted into the first port of the flask 7.

[0025] In this embodiment, the structure of the microporous filter 5 is simple, which is convenient for installation and disassembly, and convenient for the installation and removal of the filter membrane.

[0026] The flask 7 is a round-bottom flask in the prior art. A first port is arranged at the top of the flask 7, and second ports are arranged on both sides of the flask 7. One of the second ports is blocked by a plug 8, and the other second port is used for one end of the peristaltic pump 2 to extend into the flask 7. A rubber plug 6 is sleeved at the bottom of the second funnel 502, and the outer diameter of the rubber plug 6 is adapted to the inner diameter of the first port. The plug and the rubber plug 6 are prior art and will not be elaborated here.

[0027] In this embodiment, the flask 7 has a simple structure and is easy to manufacture. It can accelerate the downward penetration of water in the microporous filter 5, accelerate the water circulation in the device, and improve the filtration speed of the filter membrane. Rubber stoppers 6 and plugs are correspondingly arranged at the first port and the second port of the flask 7, which can be sealed to further enhance the osmosis effect. The second port provided with a plug can also be used as a water inlet to add water into the flask 7 during the water circulation process.

[0028] The detection and collection device further includes a constant temperature water bath 1, which is used to heat the flask 7, and the heating temperature is between 50 and 60 °C.

[0029] In this embodiment, through the heating of the constant temperature water bath 1, the flushing efficiency in the chromatography column 4 can be improved.

[0030] Working principle: Install the device, remove the plug at the second port on one side of the flask 7, and inject purified water into the flask 7, or directly install the flask 7 filled with purified water onto the device; start the constant temperature water bath 1 to heat the water in the flask 7; start the peristaltic pump 2. One end of the peristaltic pump 2 inserted into the flask 7 sucks the water in the flask 7 and transports the water to the chromatography column 4. The water flushes the resin in the chromatography column 4 and washes the particles into the microporous filter 5. The water circulates in the device. After a period of time, stop the peristaltic pump 2 and the constant temperature water bath 1, and remove the filter membrane in the microporous filter 5; compare the weight of the dried filter membrane with that of the filter membrane before filtration to obtain the mass of the particles.

[0031] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications once they know the basic creative concept. Therefore, the appended claims are intended to be construed to include the preferred embodiments as well as all changes and modifications falling within the scope of the present invention. Obviously, those skilled in the art can make various changes and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention also intends to include these modifications and variations.

Claims

1. An apparatus for collecting and detecting oligomers in macroporous adsorption resin, characterized in that, It includes a flask, a peristaltic pump, a chromatography column and a microporous filter; The flask is used for holding water; one end of the peristaltic pump is connected to the flask, and the other end is connected to the water inlet at the bottom of the chromatography column. The water outlet at the top of the chromatography column is connected to the microporous filter; the bottom end of the microporous filter is connected to the flask; the chromatography column is used for flushing the resin, and a filter membrane for intercepting particles is arranged in the microporous filter.

2. The oligomer collection and detection device for macroporous adsorption resin according to claim 1, characterized in that, The detection device further includes a constant temperature water bath, which is used for heating the water in the flask.

3. The oligomer collection and detection device for macroporous adsorption resin according to claim 1, characterized in that, The microporous filter includes a first funnel, a second funnel, a filter membrane, a dust-proof cover and a fixing clip; A dust-proof cover is arranged at the top of the first funnel, and the dust-proof cover is connected to the water outlet at the top of the chromatography column; the bottom of the first funnel and the top of the second funnel clamp the filter membrane; the fixing clip is used for connecting the first funnel and the second funnel; the bottom end of the second funnel is inserted into the flask.

4. The oligomer collection and detection device for macroporous adsorption resin according to claim 1, wherein The flask is a round-bottom flask.

5. The oligomer collection and detection device for macroporous adsorption resin according to claim 3, characterized in that, A first port is arranged at the top of the flask, and the bottom end of the second funnel is inserted into the first port.

6. The oligomer collection and detection device for macroporous adsorption resin according to claim 5, characterized in that, A rubber stopper is sleeved at the bottom end of the second funnel; the outer diameter of the rubber stopper is adapted to the inner diameter of the first port.

7. The oligomer collection and detection device for macroporous adsorption resin according to claim 5, characterized in that, Two second ports are arranged on both sides of the flask. One second port is for one end of the peristaltic pump to extend into the flask, and the other second port is provided with a plug.

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