Automatic sampling and dosing device of BCA protein quantitative kit

Abstract

The utility model discloses an automatic sampling and dosing device for a BCA protein quantitative kit, and relates to the technical field of sampling and dosing, the automatic sampling and dosing device comprises a rack and a translation mechanism, the top end of the rack is connected with a movable block in a sliding manner, the rack is fixedly provided with a placing rack, and a protective structure is arranged between the rack and the placing rack; the protection structure comprises two guide grooves, the two guide grooves are formed in the two opposite sides of the interior of the rack, a protection plate is slidably installed between the two guide grooves, and the movable block is connected with the protection plate through a transmission assembly. According to the invention, a sample, a standard working solution and a working solution for BCA protein detection which are placed in an open manner are sealed and protected during automatic sampling and dosing, bacterial contamination is avoided, a good protection effect is achieved, and the problems that all reagents are placed in an open manner in an experiment process and are easily contaminated by bacteria, and the detection efficiency is low are solved. Therefore, the stability of the reagent and the proceeding of the reaction are influenced.

Description

Technical Field

[0001] The utility model relates to the technical field of automatic sampling and dosing, in particular to an automatic sampling and dosing device for a BCA protein quantification kit. Background Art

[0002] The BCA protein quantification reagent is a reagent used to detect the protein content of a sample by the BCA protein quantification method. The BCA protein quantification method is a reagent for detecting the protein content in a sample based on a colorimetric method, and the protein content of the sample is confirmed by a standard curve drawn by a standard working solution.

[0003] In the existing BCA protein quantification method, an automatic pipetting station is usually used for automatic sampling and dosing. During the use process, there is a lack of protection for the sample, the standard working solution, and the working solution for BCA protein detection. All the reagents are placed with their openings exposed, making them easily contaminated by bacteria, which affects the stability of the reagents and the progress of the reaction. Summary of the Utility Model

[0004] The main purpose of the utility model is to provide an automatic sampling and dosing device for a BCA protein quantification kit, which can effectively solve the technical problems in the background art.

[0005] To achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0006] An automatic sampling and dosing device for a BCA protein quantification kit includes a frame and a translation mechanism. A movable block is slidably connected to the top of the frame. A placement rack is fixedly installed on the frame. A protection structure is arranged between the frame and the placement rack.

[0007] The protection structure includes two guide grooves. The two guide grooves are opened on the opposite sides inside the frame. A protection plate is slidably installed between the two guide grooves. The movable block is connected to the protection plate through a transmission component.

[0008] As a further solution of the utility model, the transmission component includes two driven racks. The two driven racks are respectively fixedly installed at both ends on one side of the protection plate. The driven racks are meshed with driven gears. The top of the driven gears is meshed with a driving gear. The top of the driving gear is meshed with a driving rack. The driving rack is fixedly connected to the movable block. A moving block is fixedly connected to the side of the driving rack close to the protection plate. A limiting hook is fixedly connected to the rear end of the moving block. Clamping blocks are fixedly connected to both sides at the top of the protection plate.

[0009] As a further solution of the utility model, a plurality of through holes are opened on the placement rack. The through holes are arranged in an array. A solution tank is opened at the top of the placement rack.

[0010] As a further solution of the present utility model, a groove adaptively matched with the limiting hook is provided at the top end of the clamping block.

[0011] As a further solution of the present utility model, an anti - detachment structure is provided on the placement rack. The anti - detachment structure includes a fixed rod and a sliding groove. The fixed rod is detachably connected to the position near the rear end of the top of the placement rack. The sliding groove is opened on one side inside the through - hole of the placement rack. An arc - shaped clamping plate is slidably installed in the sliding groove, and the arc - shaped clamping plate is elastically connected to the sliding groove through a spring.

[0012] As a further solution of the present utility model, an automatic telescopic rod is fixedly installed on the side of the movable block near the inside of the frame, and a pipette is fixedly installed on the side of the automatic telescopic rod near the inside of the frame.

[0013] The beneficial effects of the present utility model are as follows:

[0014] By setting the protection structure, after each sampling of the pipette, the protection plate can seal and protect the samples, standard working solutions, and working solutions for BCA protein detection placed open - mouthed on the placement rack, avoiding bacterial contamination. And when sampling, the protection plate automatically rises and moves, without affecting sampling, effectively enhancing the safety of the device.

[0015] By setting the anti - detachment structure, it can prevent the sealing cap of the test tube from rebounding and affecting sampling, and can fix the test tubes containing samples and standard working solutions, thus avoiding the displacement of the test tubes driven by the rising of the protection plate, effectively improving the stability of the pipetting process. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of an automatic sampling and dosing device for a BCA protein quantification kit of the present utility model;

[0017] Figure 2 It is a schematic diagram of the cross - sectional structure of an automatic sampling and dosing device for a BCA protein quantification kit of the present utility model;

[0018] Figure 3 It is a schematic diagram of the overall protection structure of an automatic sampling and dosing device for a BCA protein quantification kit of the present utility model;

[0019] Figure 4 It is a schematic diagram of the cross - sectional structure of the anti - detachment structure of an automatic sampling and dosing device for a BCA protein quantification kit of the present utility model.

[0020] In the figure: 1, frame; 2, translation mechanism; 3, movable block; 4, automatic telescopic rod; 5, pipettor; 6, placement rack; 7, protection structure; 8, anti - detachment structure; 201, motor; 202, lead screw; 203, limit rod; 701, guide groove; 702, driving rack; 703, moving block; 704, limit hook; 705, driving gear; 706, driven gear; 707, protection plate; 708, driven rack; 709, clamping block; 801, fixed rod; 802, arc - shaped clamping plate; 803, sliding groove; 804, spring. Specific embodiments

[0021] To make the technical means, creative features, achieved purposes and functions of the present utility model easy to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0022] As Figures 1-4 shown, an automatic sampling and dosing device for a BCA protein quantification kit includes a frame 1 and a translation mechanism 2. A movable block 3 is slidably connected to the top end of the frame 1. A placement rack 6 is arranged at the rear end of the frame 1. A microplate can be placed at the front end of the frame 1. The horizontal translation can be realized by means of the translation mechanism 2. The translation mechanism 2 includes a motor 201. The motor 201 is fixedly installed at the rear - end position on one side of the top end of the frame 1. A lead screw 202 and a limit rod 203 are respectively rotatably and fixedly connected to both sides of the top end of the frame 1. The lead screw 202 is fixedly connected to the output shaft of the motor 201. The lead screw 202 and the limit rod 203 are respectively thread - connected and slidably connected to the movable block 3. When the motor 201 drives the lead screw 202 to rotate, the movable block 3 can be driven to translate. The limit rod 203 plays a role in limiting and stabilizing. A protection structure 7 is arranged between the placement rack 6 and the frame 1. The protection structure 7 includes two guide grooves 701. The two guide grooves 701 are opened on the opposite sides inside the frame 1. A protection plate 707 is slidably installed between the two guide grooves 701. The movable block 3 is connected to the protection plate 707 through a transmission component. The protection structure 7 can protect the samples and reagents.

[0023] In this embodiment, the transmission assembly includes two driven racks 708. The two driven racks 708 are respectively fixedly installed at both ends on one side of the protection plate 707. The driven rack 708 is meshed with a driven gear 706. The top of the driven gear 706 is meshed with a driving gear 705. The top of the driving gear 705 is meshed with a driving rack 702. The driving rack 702 is fixedly connected to the movable block 3. The driving rack 702 is slidably connected to the guide groove 701. A moving block 703 is fixedly connected to the side of the driving rack 702 close to the protection plate 707. The moving block 703 is slidably connected to the guide groove 701. A limiting hook 704 is fixedly connected to the rear end of the moving block 703. Clamping blocks 709 are fixedly connected to both sides of the top of the protection plate 707. The transmission assembly enables the protection plate 707 to automatically move and reset during the sampling and medicine adding processes, so that the protection plate 707 does not affect sampling and medicine adding. At the same time, the guide groove 701 conforms to the movement trajectories of the transmission assembly and the protection plate 707, and can limit the protection plate 707 and the transmission assembly, making the movement of the protection plate 707 and the transmission assembly more stable and preventing them from disengaging from the guide groove 701.

[0024] In this embodiment, a plurality of through holes are formed in the penetration placement rack 6. The through holes are distributed in an array. The through holes are used to place test tubes containing standard working fluid and samples. A solution groove starts at the front end of the top of the placement rack 6. The solution groove is used to place the prepared working fluid, and the cross-section of the solution groove is an isosceles trapezoid with a larger opening at the upper end. The trapezoidal structure facilitates the transfer of the working fluid by the pipette 5.

[0025] In this embodiment, a groove adaptively matched with the limiting hook 704 is formed at the top of the clamping block 709. The limiting hook 704 has a certain elasticity, enabling the limiting hook 704 to pass through the clamping block 709 and be clamped on the rear surface of the clamping block 709, thereby driving the protection plate 707 to return during the return stroke.

[0026] In this embodiment, an anti-disengagement structure 8 is provided on the placement rack 6. The anti-disengagement structure 8 includes a fixed rod 801 and a sliding groove 803. One end of the fixed rod 801 is rotatably connected to the placement rack 6, and the other end is fixed to the placement rack 6 by a bolt, thus facilitating the disassembly and assembly of one end of the fixed rod 801. The fixed rod 801 can fix the sealing cap of the test tube to prevent the sealing cap from rebounding and affecting sampling. The sliding groove 803 is formed in the through hole of the placement rack 6. An arc-shaped clamping plate 802 is slidably installed in the sliding groove 803. A spring 804 is fixedly connected to the side of the arc-shaped clamping plate 802 close to the sliding groove 803. The other end of the spring 804 is fixedly connected to the inner wall of the sliding groove 803. The spring 804 applies pressure to the arc-shaped clamping plate 802, causing the arc-shaped clamping plate 802 to tightly press against the test tube. When the protection plate 707 disengages from the test tube, it will not drive the test tube to disengage from the placement rack 6, improving the stability during operation.

[0027] In this embodiment, an automatic telescopic rod 4 is fixedly installed on the inner side of the movable block 3 close to the frame 1, and a pipette 5 is fixedly installed on the inner side of the automatic telescopic rod 4 close to the frame 1. The pipette 5 is a multi-channel pipette, which can reduce the operation time. The automatic telescopic rod 4 enables the pipette 5 to achieve displacement in the vertical direction, and in cooperation with the translation mechanism 2, it can achieve the functions of sampling and adding medicine.

[0028] It should be noted that the present utility model is an automatic sampling and adding medicine device for a BCA protein quantification kit. When in use, the microplate is fixed in the frame 1, and then the diluted samples and standard working solutions are placed in the openings of the placement rack 6. The fixing rod 801 is opened to fix the sealing caps of all test tubes to prevent rebound from affecting sampling. Then, the prepared working solution is added into the grooves of the placement rack 6. By starting the motor 201, the motor 201 drives the lead screw 202 to rotate, thereby driving the displacement of the movable block 3, and further driving the pipette 5 to move horizontally. The limiting rod 203 plays a limiting role. The automatic telescopic rod 4 can drive the pipette 5 to move up and down, so as to automatically perform liquid transfer, thereby realizing the automatic sampling and adding medicine of the BCA protein quantification kit.

[0029] During sampling, the movable block 3 moves backward, driving the active rack 702 to slide along the guide groove 701. After the active rack 702 meshes with the active gear 705, it drives the active gear 705 to rotate. The active gear 705 drives the driven gear 706 to rotate, and the driven gear 706 drives the driven rack 708 to rotate, thereby driving the protective plate 707 to move upward. When the protective plate 707 moves upward, it will drive the test tube to have an upward movement trend. At this time, under the action of the elastic force of the spring 804, the arc-shaped clamping plate 802 displaces along the sliding groove 803, so as to tightly abut against the surface of the test tube, playing a role in fastening the test tube. Under the combined action of the fixing rod 801, the test tube is prevented from moving upward. When the movable block 3 continues to move backward, the moving block 703 contacts and abuts against the clamping block 709, and the limiting hook 704 passes through the clamping block 709 and abuts against the other end of the clamping block 709, thereby driving the protective plate 707 to move backward, without affecting sampling. When the transfer is carried out after sampling, the limiting hook 704 will drive the protective plate 707 to move to the top of the placement rack 6, and under the reverse action of the active rack 702, the protective plate 707 is reset to play a protective role.

[0030] The above shows and describes the basic principles, main features and advantages of the present utility model. Those skilled in the art of this industry should understand that the present utility model is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed. The scope of protection claimed by the present utility model is defined by the appended claims and their equivalents.

Claims

1. An automatic sampling and dosing device for a BCA protein quantification kit, comprising a frame (1) and a translation mechanism (2). A movable block (3) is slidably connected to the top of the frame (1), and a placement rack (6) is fixedly installed on the frame (1). It is characterized in that: A protective structure (7) is provided between the frame (1) and the placement rack (6); The protective structure (7) includes two guide grooves (701). The two guide grooves (701) are opened on the opposite sides inside the frame (1). A protective plate (707) is slidably installed between the two guide grooves (701). The movable block (3) is connected to the protective plate (707) through a transmission assembly.

2. The automatic sampling and drug adding device for a BCA protein quantification kit according to claim 1, characterized in that: The transmission assembly includes two driven racks (708). The two driven racks (708) are respectively fixedly installed at both ends on one side of the protective plate (707). The driven rack (708) is meshed with a driven gear (706). The top of the driven gear (706) is meshed with a driving gear (705). The top of the driving gear (705) is meshed with a driving rack (702). The driving rack (702) is fixedly connected to the movable block (3). A moving block (703) is fixedly connected to the side of the driving rack (702) close to the protective plate (707). A limiting hook (704) is fixedly connected to the rear end of the moving block (703). Clamping blocks (709) are fixedly connected to both sides at the top of the protective plate (707).

3. The automatic sampling and drug addition device for a BCA protein quantification kit according to claim 1, characterized in that: A number of through holes are opened on the placement rack (6). The through holes are distributed in an array. A solution tank is opened at the top of the placement rack (6).

4. The automatic sampling and drug adding device for a BCA protein quantification kit according to claim 2, characterized in that: A groove adapted to the limiting hook (704) is opened at the top of the clamping block (709).

5. The automatic sampling and drug adding device for a BCA protein quantification kit according to claim 1, characterized in that: An anti - detachment structure (8) is provided on the placement rack (6). The anti - detachment structure (8) includes a fixed rod (801) and a sliding groove (803). The fixed rod (801) is detachably connected to the position near the rear end of the top of the placement rack (6). The sliding groove (803) is opened on one side inside the through hole of the placement rack (6). An arc - shaped clamping plate (802) is slidably installed in the sliding groove (803). The arc - shaped clamping plate (802) is elastically connected to the sliding groove (803) through a spring (804).

6. The automatic sampling and drug adding device for a BCA protein quantification kit according to claim 1, characterized in that: An automatic telescopic rod (4) is fixedly installed on the side of the movable block (3) close to the inside of the frame (1). A pipette (5) is fixedly installed on the side of the automatic telescopic rod (4) close to the inside of the frame (1).

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