Reagent card for immunoblotting allergen specificity IgE antibody detection

Abstract

The utility model discloses a reagent card for detecting allergen-specific IgE (Immunoglobulin E) antibodies by using an immunoblotting method, and relates to the technical field of IgE antibody detection. The reagent card comprises a reagent card body, the reagent card body comprises a reaction membrane strip and an incubation plate, grabbing mechanisms are arranged on the two sides of the top of the reaction membrane strip, each grabbing mechanism comprises an extension part, a supporting part is arranged on the top of each extension part, a grabbing part is arranged on the top of each supporting part, and the reaction membrane strip is arranged on the reaction membrane strip. According to the incubation plate disclosed by the utility model, the extension part, the supporting part and the grabbing part are arranged, the supporting part and the grabbing part are fixed on the extension part, and when a reaction film strip is placed, the whole grabbing part extends out of the object placing groove and protrudes out of the surface of the incubation plate, so that a detector can conveniently take out the reaction film strip from the object placing groove; the whole grabbing part does not make contact with a solution in the incubation process and is kept dry, meanwhile, when a detector takes out the reaction film strip, the detector does not make contact with the reaction film strip, and the accuracy of the detection result can be improved to a certain degree.

Description

Technical Field

[0001] The utility model relates to the technical field of IgE antibody detection, in particular to a reagent card for detecting allergen-specific IgE antibodies by immunoblotting method. Background Technique

[0002] Immunoblotting is a common technique for detecting allergen-specific IgE antibodies, which combines high-resolution gel electrophoresis and immunochemical analysis techniques. Protein samples such as antigens are electrophoretically separated in a gel after treatment, and then transferred to a membrane. Specific antibodies react with the proteins on the membrane, and then an enzyme-labeled secondary antibody reacts with a substrate to produce a color reaction, thereby detecting specific allergen-specific IgE antibodies. Reagent cards for detecting allergen-specific IgE antibodies usually include reaction membrane strips, incubation plates, and various solutions, such as concentrated washing solutions, enzyme working solutions, substrate solutions, and color comparison cards for comparison.

[0003] When the existing reagent cards for detecting allergen-specific IgE antibodies are in use, after placing the numbers on the reaction membrane strip corresponding to the numbers on the incubation plate, they are washed, and a sample that completely submerges the reaction membrane strip is added for multiple oscillatory incubations. During multiple incubations, an appropriate amount of IgE antibody, the corresponding enzyme working solution, and substrate working solution are added. Finally, after the washing is completed, the reaction membrane strip is taken out and dried for color comparison. During the whole process, the reaction membrane strip will come into contact with various solutions, and the solutions need to completely infiltrate the reaction membrane strip, which requires the placement slots on the incubation plate to have sufficient depth. After the reaction membrane strip is placed in a sufficiently deep placement slot, it is not easy to take out, and when taking it out of the placement slot, it is accompanied by the direct contact of the tester's hand with the reaction membrane strip, and the accuracy of the detection result needs to be further improved. Summary of the Utility Model

[0004] Based on this, the purpose of the utility model is to provide a reagent card for detecting allergen-specific IgE antibodies by immunoblotting method, so as to solve the technical problems that in the use process of the existing reagent cards for detecting allergen-specific IgE antibodies by immunoblotting method, the reaction membrane strip is not easy to take out, and the accuracy of the detection result needs to be further improved.

[0005] To achieve the above purpose, the utility model provides the following technical solution: A reagent card for detecting allergen-specific IgE antibodies by immunoblotting method, including a reagent card main body, the reagent card main body includes a reaction membrane strip and an incubation plate, grasping mechanisms are arranged on both sides of the top of the reaction membrane strip, the grasping mechanisms include extension parts, a supporting part is arranged on the top of the extension parts, a grasping part is arranged on the top of the supporting part, clamping blocks are arranged on both sides of the grasping part, a card seat is arranged on the top of the incubation plate, a card slot is opened on the card seat, and a placement slot is opened on the incubation plate.

[0006] By adopting the above technical solution, the setting of the grasping mechanism enables the tester to take out the reaction membrane strip from the storage slot of the incubation plate through the grasping mechanism after the reaction of the reaction membrane strip is completed.

[0007] Furthermore, there are two groups of the grasping mechanisms, and the two groups of the grasping mechanisms are symmetrically arranged. There are two groups of the supporting parts, and each group of the supporting parts has two. There is a through groove between the two supporting parts.

[0008] By adopting the above technical solution, the symmetrical setting of the grasping mechanism, especially its extension part, supporting part and grasping part, provides a stable grasping method for the experimenter, making the grasping of the reagent card more convenient, balanced and stable, preventing possible tilting or slipping during the operation, and improving the safety and reliability of the operation.

[0009] Furthermore, there are multiple storage slots, and the multiple storage slots are evenly and equidistantly distributed. There are multiple groups of the card seats, and each group of the card seats has four. Two of the card seats are symmetrically arranged with the other two card seats along the storage slot.

[0010] By adopting the above technical solution, the multiple evenly and equidistantly distributed storage slots can process multiple samples simultaneously, improving the efficiency of the experiment.

[0011] Furthermore, the extension part is fixedly connected to the reaction membrane strip, the extension part is fixedly connected to the supporting part, the supporting part is fixedly connected to the grasping part, and the grasping part is fixedly connected to the block.

[0012] By adopting the above technical solution, the fixed connection method between the extension part, the supporting part and the grasping part ensures the stability and durability of the structure of the reagent card.

[0013] Furthermore, the card seat is fixedly connected to the incubation plate, and the block is engaged and slidable with the card slot.

[0014] By adopting the above technical solution, fixing the card seat on the incubation plate can utilize the incubation plate to provide sufficient supporting force for it.

[0015] Furthermore, both the reaction membrane strip and the extension part are adapted to the storage slot, and the sum of the thickness of the reaction membrane strip and the height of the supporting part is equal to the depth of the storage slot.

[0016] By adopting the above technical solution, the high adaptability of the reaction membrane strip and the extension part to the storage slot enables the whole formed by the reaction membrane strip and the extension part to be smoothly placed in the storage slot.

[0017] Furthermore, anti-overflow grooves are formed on both sides of the top of the incubation plate. The anti-overflow grooves are located on both sides of the storage slot and are communicated with the storage slot.

[0018] By adopting the above technical solution, when the reaction membrane strip is oscillated and incubated multiple times and the entire incubation plate tilts with the detection device, the solution submerging the reaction membrane strip can enter the anti-overflow tank from the through groove over the arc portion at the top of the baffle block.

[0019] Furthermore, a baffle block is provided at the bottom of the connection between the anti-overflow tank and the placement tank, and the baffle block is closely attached to the bottom of the connection between the anti-overflow tank and the placement tank.

[0020] By adopting the above technical solution, the setting of the baffle block can prompt the amount of solution added during the detection process, avoiding unnecessary waste caused by excessive addition of the solution.

[0021] Furthermore, an arc portion is provided at the top of the baffle block, and the arc portion has a semi-circular structure.

[0022] By adopting the above technical solution, the setting of the arc portion can guide the excess part of the solution to smoothly flow into the anti-overflow tank due to tilting during the oscillating incubation process.

[0023] Furthermore, a number is provided on one side of the top of the incubation plate, and the numbers on the number increase sequentially.

[0024] By adopting the above technical solution, the design of the number facilitates the management and marking of multiple reaction membrane strips, avoiding confusion or errors during the experiment.

[0025] In summary, the present utility model mainly has the following beneficial effects:

[0026] 1. By providing an extension part, a support part and a grasping part, the support part and the grasping part are fixed on the extension part, and when placing the reaction membrane strip, the whole grasping part protrudes from the placement tank and protrudes above the surface of the incubation plate, facilitating the detection personnel to take out the reaction membrane strip from the placement tank. The whole grasping part will not contact the solution at all during the incubation process and remains dry. At the same time, when the detection personnel take out the reaction membrane strip, they will not touch the reaction membrane strip itself, which can improve the accuracy of the detection result to a certain extent.

[0027] 2. By providing a through groove, a baffle block, an arc portion and an anti-overflow tank, when the reaction membrane strip is oscillated and incubated multiple times and the entire incubation plate tilts with the detection device, the solution submerging the reaction membrane strip can enter the anti-overflow tank from the through groove over the arc portion at the top of the baffle block, avoiding the solution flowing out due to oscillation during incubation and causing pollution of the detection device. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a three-dimensional structural schematic diagram of the present utility model;

[0029] Figure 2Schematic top-down three-dimensional structure diagram of the present utility model;

[0030] Figure 3 Schematic three-dimensional structure diagram of the reaction membrane strip of the present utility model;

[0031] Figure 4 For the present utility model Figure 2 Enlarged structure diagram at position A in the present utility model.

[0032] In the figure: 1. Reagent card main body; 11. Reaction membrane strip; 12. Incubation plate; 2. Gripping mechanism; 21. Extension part; 22. Support part; 23. Through groove; 24. Gripping part; 25. Clamping block; 26. Clamping seat; 27. Clamping groove; 3. Placing groove; 4. Anti-overflow groove; 5. Block; 6. Arc part; 7. Number. Specific embodiments

[0033] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. The embodiments described below by referring to the accompanying drawings are exemplary and are only used to explain the present utility model and should not be construed as limiting the present utility model.

[0034] Next, the embodiments of the present utility model will be described according to the overall structure of the present utility model.

[0035] Embodiment 1:

[0036] A reagent card for detecting allergen-specific IgE antibodies by immunoblotting method, as Figures 1 - 4 shown, includes a reagent card main body 1. The reagent card main body 1 includes a reaction membrane strip 11 and an incubation plate 12. Gripping mechanisms 2 are provided on both sides of the top of the reaction membrane strip 11. The gripping mechanisms 2 include extension parts 21. Support parts 22 are provided on the tops of the extension parts 21. Gripping parts 24 are provided on the tops of the support parts 22. Clamping blocks 25 are provided on both sides of the gripping parts 24. Clamping seats 26 are provided on the top of the incubation plate 12. Clamping grooves 27 are opened on the clamping seats 26. A placing groove 3 is opened on the incubation plate 12. The setting of the gripping mechanisms 2 enables the tester to take out the reaction membrane strip 11 from the placing groove 3 of the incubation plate 12 through the gripping mechanisms 2 after the reaction of the reaction membrane strip 11 is completed. Compared with the method of the tester directly reaching into the deep and narrow placing groove 3 to take out the reaction membrane strip 11, this method is simpler and faster, and the hand will not directly contact the reaction membrane strip 11, which can simplify the extraction process of the reaction membrane strip 11 to a certain extent and improve the accuracy of the detection result.

[0037] Refer to Figure 1 、 Figure 2 、 Figure 3, there are two sets of the grasping mechanisms 2, and the two sets of the grasping mechanisms 2 are symmetrically arranged. There are two sets of the supporting parts 22, and each set of the supporting parts 22 has two. There is a through groove 23 between the two supporting parts 22. The symmetrical arrangement of the grasping mechanism 2, especially its extending part 21, supporting part 22 and grasping part 24, provides a stable grasping method for the experimenter, making the grasping of the reagent card more convenient, balanced and stable, preventing tilting or slipping that may occur during the operation, and improving the safety and reliability of the operation. The setting of the through groove 23 enables the surplus part of the solution that wets the reaction membrane strip 11 to flow into the anti-overflow groove 4 through the through groove 23 temporarily over the arc part 6 at the top of the baffle 5 when the reaction membrane strip 11 and the incubation plate 12 are tilted during the oscillating incubation, preventing the solution from flowing out.

[0038] Refer to Figure 1 、 Figure 2 , there are multiple placement grooves 3, and the multiple placement grooves 3 are evenly and equidistantly distributed. There are multiple groups of the card seats 26, and each group of the card seats 26 has four. Two of the card seats 26 and the other two card seats 26 are symmetrically arranged along the placement groove 3. The multiple evenly and equidistantly distributed placement grooves 3 can process multiple samples simultaneously, improving the efficiency of the experiment. The setting of the card seats 26 and the card blocks 25 can conveniently fix the reaction membrane strip 11, preventing the incubation plate 12 from tilting during the oscillating incubation, and avoiding the overall movement of the reaction membrane strip 11 and the grasping mechanism 2 when the surplus solution impacts the supporting part 22.

[0039] Refer to Figure 1 、 Figure 2 、 Figure 3 , the extending part 21 is fixedly connected to the reaction membrane strip 11, the extending part 21 is fixedly connected to the supporting part 22, the supporting part 22 is fixedly connected to the grasping part 24, and the grasping part 24 is fixedly connected to the card block 25. The fixed connection method between the extending part 21, the supporting part 22 and the grasping part 24 ensures the stability and durability of the reagent card structure. At the same time, when the surplus solution impacts the supporting part 22 due to the tilting of the incubation plate 12 during the oscillating incubation, the connection relationship between the card block 25 and the card slot 27 is used to keep the reaction membrane strip 11 and other components of the grasping mechanism 2 in a stable position.

[0040] Refer to Figure 1 、 Figure 2 、 Figure 3 、 Figure 4, the card seat 26 is fixedly connected to the incubation plate 12, and the card block 25 is engaged and slid with the card slot 27 to fix the card seat 26 on the incubation plate 12. The incubation plate 12 can provide sufficient supporting force for it. The design of the card block 25 engaged with the card slot 27 not only enables the reaction membrane strip 11 to be conveniently and stably fixed on the incubation plate 12, but also simplifies the experimental operation and avoids accidental movement of the reaction membrane strip 11 during the experiment.

[0041] Refer to Figure 1 , Figure 2 , the reaction membrane strip 11 and the extension part 21 are both adapted to the storage groove 3, and the sum of the thickness of the reaction membrane strip 11 and the height of the support part 22 is equal to the depth of the storage groove 3. The high adaptability of the reaction membrane strip 11 and the extension part 21 to the storage groove 3 enables the whole formed by the reaction membrane strip 11 and the extension part 21 to be smoothly placed in the storage groove 3. The setting of the depth of the storage groove 3 enables the grasping part 24 to be located above the storage groove 3 when the reaction membrane strip 11 is placed in the storage groove 3, avoiding the solution from contacting the grasping part 24.

[0042] Embodiment 2:

[0043] Refer to Figure 1 , Figure 2 , Figure 4 , both sides of the top of the incubation plate 12 are provided with anti-overflow grooves 4. The anti-overflow grooves 4 are located on both sides of the storage groove 3 and are communicated with the storage groove 3. When the reaction membrane strip 11 is incubated by oscillation for many times and the whole incubation plate 12 tilts with the detection device, the solution submerging the reaction membrane strip 11 can enter the anti-overflow groove 4 from the through groove 23 over the arc part 6 at the top of the baffle 5, effectively preventing the solution from overflowing during the incubation process and ensuring the cleanliness and accuracy of the experiment.

[0044] Refer to Figure 1 , Figure 2 , Figure 4 , a baffle 5 is arranged at the bottom of the connection between the anti-overflow groove 4 and the storage groove 3. The baffle 5 is closely attached to the bottom of the connection between the anti-overflow groove 4 and the storage groove 3. The setting of the baffle 5 can prompt the amount of solution added during the detection process, avoid unnecessary waste caused by excessive solution addition, and at the same time, the baffle 5 is closely attached to the bottom of the connection between the anti-overflow groove 4 and the storage groove 3 to ensure that when the solution wets the reaction membrane strip 11, it will not leak from the bottom of the storage groove 3 to the anti-overflow groove 4.

[0045] Refer to Figure 1 , Figure 2 , Figure 4, an arc portion 6 is provided at the top of the stopper 5. The arc portion 6 has a semi-circular structure. The setting of the arc portion 6 can guide the excess solution to smoothly flow into the anti-overflow tank 4 due to the inclination during the oscillating incubation process, avoiding the accumulation of the solution on the top of the stopper 5, thus ensuring the anti-overflow effect and better guaranteeing the cleanliness of the detection equipment.

[0046] Refer to Figure 1 , Figure 2 , a number 7 is provided on one side of the top of the incubation plate 12. The numbers on the number 7 increase sequentially. The design of the number 7 facilitates the management and marking of multiple reaction membrane strips 11, avoiding confusion or errors during the detection process, further improving the accuracy and efficiency of the experiment. The sequential increase of the numbers on the number 7 also facilitates the experimenter to sort and search for the reaction membrane strips 11.

[0047] The implementation principle of the present utility model is as follows: First, the tester prepares the required solution, takes out the reaction membrane strip 11, holds the gripping portion 24 at the top of the extension portion 21 and the supporting portion 22, aligns the clamping block 25 with the card slot 27 on the card seat 26, and places the corresponding reaction membrane strip 11 into the placement slot 3 corresponding to the number 7. At this time, the corresponding clamping block 25 just completely inserts into the card slot 27; Second, place the incubation plate 12 with the reaction membrane strip 11 placed in the corresponding position of the detection equipment, and use the detection equipment to inject cleaning liquid or other reaction solutions according to the corresponding process. The solution needs to completely soak the reaction membrane strip 11 and perform oscillating incubation. During the oscillating incubation process, the incubation plate 12 will tilt. If too much solution for soaking the reaction membrane strip 11 is injected, when tilting, the excess part will cross the arc portion 6 at the top of the stopper 5 and enter the anti-overflow tank 4, preventing the solution from flowing out of the incubation plate 12 due to oscillation; Finally, when all the processes are completed, the tester holds the gripping portion 24, pulls the clamping block 25 out of the card slot 27 of the card seat 26, and further takes out the reaction membrane strip 11 from the deeper placement slot 3. The taking-out process is simple and fast, and the tester's hand does not contact the reaction membrane strip 11 at all. After that, perform colorimetry after it dries.

[0048] Parts not involved in the present utility model are the same as or can be implemented by the prior art, and will not be elaborated here.

[0049] Although the embodiments of the present utility model have been shown and described, the specific embodiments are only explanations of the present utility model and not limitations thereof. The specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art can make modifications, substitutions and variations without creative contributions to the embodiments according to needs, but as long as they are within the scope of the claims of the present utility model, they are protected by the patent law.

Claims

1. A reagent card for detecting allergen-specific IgE antibodies by immunoblotting, characterized in that: It includes a reagent card body (1), the reagent card body (1) includes a reaction membrane strip (11) and an incubation plate (12), gripping mechanisms (2) are arranged on both sides of the top of the reaction membrane strip (11), the gripping mechanisms (2) include extension parts (21), a support part (22) is arranged on the top of the extension part (21), a gripping part (24) is arranged on the top of the support part (22), clamping blocks (25) are arranged on both sides of the gripping part (24), a card seat (26) is arranged on the top of the incubation plate (12), a card slot (27) is formed in the card seat (26), and a storage groove (3) is formed in the incubation plate (12).

2. The reagent card for detecting allergen-specific IgE antibodies by immunoblotting according to claim 1, characterized in that: Two groups of the gripping mechanisms (2) are provided, and the two groups of the gripping mechanisms (2) are symmetrically arranged. Two groups of the support parts (22) are provided, and each group of the support parts (22) has two. A through groove (23) is arranged between the two support parts (22).

3. The reagent card for detecting allergen-specific IgE antibodies by immunoblotting according to claim 1, characterized in that: A plurality of the storage grooves (3) are provided, and the plurality of the storage grooves (3) are evenly and equidistantly distributed. A plurality of groups of the card seats (26) are provided, and each group of the card seats (26) has four. Two of the card seats (26) and the other two card seats (26) are symmetrically arranged along the storage groove (3).

4. The reagent card for detecting allergen-specific IgE antibody by immunoblotting method according to claim 1, characterized in that: The extension part (21) is fixedly connected to the reaction membrane strip (11), the extension part (21) is fixedly connected to the support part (22), the support part (22) is fixedly connected to the gripping part (24), and the gripping part (24) is fixedly connected to the clamping block (25).

5. The reagent card for detecting allergen-specific IgE antibodies by immunoblotting according to claim 1, characterized in that: The card seat (26) is fixedly connected to the incubation plate (12), and the clamping block (25) is engaged and slidable with the card slot (27).

6. The reagent card for detecting allergen-specific IgE antibodies by immunoblotting according to claim 1, characterized in that: The reaction membrane strip (11) and the extension part (21) are both adapted to the storage groove (3), and the sum of the thickness of the reaction membrane strip (11) and the height of the support part (22) is equal to the depth of the storage groove (3).

7. The reagent card for detecting allergen-specific IgE antibodies by immunoblotting according to claim 1, characterized in that: Overflow prevention grooves (4) are formed on both sides of the top of the incubation plate (12), the overflow prevention grooves (4) are located on both sides of the storage groove (3), and the overflow prevention grooves (4) are communicated with the storage groove (3).

8. The reagent card for detecting allergen-specific IgE antibodies by immunoblotting according to claim 7, wherein: A blocking block (5) is arranged at the bottom of the connection between the overflow prevention groove (4) and the storage groove (3), and the blocking block (5) is closely attached to the bottom of the connection between the overflow prevention groove (4) and the storage groove (3).

9. The reagent card for detecting allergen-specific IgE antibodies by immunoblotting according to claim 8, wherein: An arc part (6) is arranged on the top of the blocking block (5), and the arc part (6) has a semi-circular structure.

10. The reagent card for detecting allergen-specific IgE antibodies by immunoblotting according to claim 1, characterized in that: A number (7) is arranged on one side of the top of the incubation plate (12), and the numbers on the number (7) increase in sequence.

Images