Transfer film box for protein imprinting method

Abstract

The utility model belongs to the technical field of biochemical experimental equipment, and discloses a transfer film box for a protein imprinting method, which comprises a rectangular transfer film groove, an electrode a and an electrode b are respectively arranged on two sides in the transfer film groove and are used for providing an electric field in the transfer film process, and light lamps are uniformly distributed on the top of the opposite inner walls of the transfer film groove and are used for supplying light to the transfer film groove. A switch is arranged in the middle of the top of the inner wall of the long edge of one side of the membrane transfer tank, and rubber stabilizing pads are arranged at four corners of the bottom of the membrane transfer tank, so that the temperature in the membrane transfer process is reduced, the influence of heat on separation glue and a PVDF membrane is reduced, and the accuracy and the stability of an experimental result are ensured; the influence of room temperature change is avoided during repeated experiments, and the homogeneity of the repeated experiments is ensured.

Description

Technical Field

[0001] This application relates to the technical field of biochemical experimental equipment, and more specifically, to a membrane transfer cassette for protein blotting. Background Art

[0002] In molecular biology experiments, the protein blotting (Western Blot) is a commonly used technique for detecting protein expression. Among them, the membrane transfer step is a crucial step in transferring the proteins separated by electrophoresis from the gel to a solid-phase carrier (such as a PVDF membrane or an NC membrane). However, traditional membrane transfer methods are usually affected by the heat generated by the current, resulting in changes in the properties and performance of the separating gel and the PVDF membrane (or NC membrane), thereby affecting the experimental results.

[0003] To overcome the above problems, the present invention provides a membrane transfer cassette for protein blotting, which reduces the temperature during the membrane transfer process, reduces the influence of heat on the separating gel and the PVDF membrane (or NC membrane), and ensures the accuracy and stability of the experimental results. To solve the above problems, this application provides a membrane transfer cassette for protein blotting. Utility Model Content

[0004] The membrane transfer cassette for protein blotting provided by this application adopts the following technical solutions:

[0005] A membrane transfer cassette for protein blotting includes a rectangular membrane transfer tank. Electrodes a and b are respectively arranged on both inner sides of the membrane transfer tank for providing an electric field during the membrane transfer process. Light lamps are evenly distributed at the top between the opposite inner walls of the membrane transfer tank. A switch is arranged at the middle position of the top of the inner wall of one long side of the membrane transfer tank. Rubber stabilizing pads are arranged at the four corners of the bottom of the membrane transfer tank.

[0006] Furthermore, ice bath tanks a and b are respectively arranged on both outer sides of the membrane transfer tank. Ice bath tanks a and b jointly surround the membrane transfer tank to form a hollow "rectangular" structure for reducing the temperature during the membrane transfer process.

[0007] Furthermore, a water outlet pipe a with a valve is arranged on the outer side of ice bath tank a, and a water outlet pipe b with a valve is arranged on the outer side of ice bath tank b for discharging the ice bath liquid.

[0008] Furthermore, two convex columns are vertically arranged on both inner sides of the membrane transfer tank. Electrodes a and b are respectively arranged inside the two convex columns located in the middle position to ensure the uniform transfer of proteins from the gel to the PVDF membrane.

[0009] Furthermore, ice bath tanks a and b are filled with an ice bath liquid, which is a mixture of ice and water or any liquid suitable for reducing the temperature to maintain a lower temperature environment during the membrane transfer process.

[0010] Furthermore, the membrane transfer cassette further includes a top cover for covering the membrane transfer tank to enhance the sealing performance and cooling effect during the membrane transfer process.

[0011] Furthermore, the membrane transfer cassette is made of a material with good low-temperature resistance, corrosion resistance, and insulation performance to ensure stability and safety in a low-temperature environment.

[0012] In summary, the present application includes the following beneficial technical effects:

[0013] Reduce the temperature during the membrane transfer process, minimize the impact of heat on the separation gel and PVDF membrane, ensure the accuracy and stability of experimental results. The design of the ice bath tank keeps the transfer buffer at a low and stable temperature, unaffected by room temperature changes during repeated experiments, ensuring the homogeneity of repeated experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a schematic diagram of the overall structure of the present application;

[0015] Figure 2 is a schematic diagram of the bottom view structure of the present application.

[0016] Explanation of the reference numerals in the drawings:

[0017] 1. Membrane transfer tank; 2. Electrode a; 3. Electrode b; 4. Ice bath tank a; 5. Ice bath tank b; 6. Water outlet pipe a; 7. Water outlet pipe b; 8. Convex column; 9. Light lamp; 10. Switch; 11. Rubber stabilizing pad. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0018] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0019] In the description of the present application, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer", "top / bottom end", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0020] In the description of the present application, it should be noted that unless otherwise clearly defined and limited, terms such as "installation", "equipped with", "sheathed / connected", "connection", etc. should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0021] Embodiment:

[0022] An embodiment of the present application discloses a membrane transfer cassette for Western blotting. Please refer to Figure 1 - Figure 2 , which includes a rectangular membrane transfer tank 1. On both sides inside the membrane transfer tank, there are respectively an electrode a 2 and an electrode b 3 for providing an electric field during the membrane transfer process. On the top between the opposite inner walls of the membrane transfer tank 1, there are evenly distributed light lamps 9. In the middle position at the top of the inner wall on one long side of the membrane transfer tank 1, there is a switch 10. At the four corners of the bottom of the membrane transfer tank 1, there are rubber stabilizing pads 11. On both sides inside the membrane transfer tank 1, there are respectively an electrode a 2 and an electrode b 3. These two electrodes provide an electric field during the membrane transfer process, which helps the effective transfer or treatment of the membrane. On the top between the opposite inner walls of the membrane transfer tank 1, there are evenly distributed light lamps 9. These light lamps provide necessary illumination for the membrane transfer process, enabling the operator to clearly observe the membrane transfer process, and may also help monitor the effect of the membrane transfer. In the middle position at the top of the inner wall on one long side of the membrane transfer tank 1, there is a switch 10. This switch may be used to control the opening and closing of the electric field and the light lamps, providing a convenient operation interface for the operator. At the four corners of the bottom of the membrane transfer tank 1, there are rubber stabilizing pads 11. These stabilizing pads increase the friction between the membrane transfer tank and the placement surface, effectively preventing the membrane transfer tank from sliding or moving during the operation, and ensuring the stability of the membrane transfer process.

[0023] On both outer sides of the membrane transfer tank 1, there are respectively an ice bath tank a 4 and an ice bath tank b 5. The ice bath tank a 4 and the ice bath tank b 5 jointly surround the membrane transfer tank 1 to form a hollow "rectangular" structure for reducing the temperature during the membrane transfer process. On both outer sides of the membrane transfer tank 1, there are respectively an ice bath tank a 4 and an ice bath tank b 5. These two ice bath tanks jointly surround the membrane transfer tank 1 to form a hollow "rectangular" structure. Such a design can reduce the temperature during the membrane transfer process, provide a constant low-temperature environment for the membrane transfer process, help maintain the stability during the membrane transfer process, and may improve the membrane transfer efficiency.

[0024] On the outer side of the ice bath tank a 4, there is a water outlet pipe a 6 with a valve, and on the outer side of the ice bath tank b 5, there is a water outlet pipe b 7 with a valve for discharging the ice bath liquid.

[0025] On both inner sides of the membrane transfer tank 1, two convex columns 8 are vertically provided. Electrodes a2 and b3 are respectively arranged inside the two convex columns located in the middle position to ensure the uniform transfer of proteins from the gel to the PVDF membrane or NC membrane.

[0026] The ice bath tank a4 and the ice bath tank b5 are filled with an ice bath liquid, which is an ice-water mixture or any liquid suitable for reducing the temperature, so as to maintain a low-temperature environment during the membrane transfer process. The membrane transfer box further includes a top cover for covering the membrane transfer tank 1 to increase the sealing performance and cooling effect during the membrane transfer process.

[0027] The membrane transfer box is made of a material with good low-temperature resistance, corrosion resistance and insulation performance to ensure stability and safety in a low-temperature environment. The overall design of the membrane transfer tank comprehensively considers multiple aspects of the membrane transfer process, including electric field supply, temperature control, lighting requirements, operation convenience and stability. These design elements together ensure the efficiency, safety and stability of the membrane transfer process, and at the same time provide flexibility and scalability for the diverse needs of experiments or production.

[0028] The implementation principle of this embodiment is as follows: Place the gel containing proteins between the electrodes of the membrane transfer tank, cover the PVDF membrane (or NC membrane) on the gel, add the membrane transfer liquid, open the valve of the ice bath tank to add the ice bath liquid, close the valve and turn on the power supply to complete the transfer of proteins, and discharge the ice bath liquid through the water outlet pipe.

[0029] The above are all the preferred embodiments of this application, and the protection scope of this application is not limited accordingly. Therefore, all equivalent changes made according to the structure, shape and principle of this application shall be covered within the protection scope of this application.

Claims

1. A membrane transfer cassette for Western blotting, characterized in that, It includes a rectangular membrane transfer tank (1). On both inner sides of the membrane transfer tank, there are electrode a (2) and electrode b (3) respectively, which are used to provide an electric field during the membrane transfer process. On the top between the opposite inner walls of the membrane transfer tank (1), there are evenly distributed light lamps (9). In the middle position at the top of the inner wall of one long side of the membrane transfer tank (1), there is a switch (10). At the four corners of the bottom of the membrane transfer tank (1), there are rubber stabilizing pads (11).

2. The membrane transfer cassette for Western blotting according to claim 1, characterized in that, On both outer sides of the membrane transfer tank (1), there are ice bath tank a (4) and ice bath tank b (5) respectively. Ice bath tank a (4) and ice bath tank b (5) jointly surround the membrane transfer tank (1) on all sides, forming a hollow "rectangular" structure, which is used to reduce the temperature during the membrane transfer process.

3. The transfer cassette for Western blotting according to claim 2, wherein On the outer side of ice bath tank a (4), there is a water outlet pipe a (6) with a valve. On the outer side of ice bath tank b (5), there is a water outlet pipe b (7) with a valve, which is used to discharge the ice bath liquid.

4. A membrane transfer cassette for Western blotting according to claim 3, wherein, On both inner sides of the membrane transfer tank (1), there are two convex columns (8) vertically arranged. Inside the two convex columns located in the middle position, there are electrode a (2) and electrode b (3) respectively, ensuring the uniform transfer of proteins from the gel to the PVDF membrane or NC membrane.

5. A membrane transfer cassette for Western blotting according to claim 4, wherein, Ice bath tank a (4) and ice bath tank b (5) are filled with an ice bath liquid, which is an ice - water mixture or any liquid suitable for reducing the temperature, so as to maintain a low - temperature environment during the membrane transfer process.

6. A membrane transfer cassette for Western blotting according to claim 5, wherein, This membrane transfer box also includes a top cover used to cover the membrane transfer tank (1), so as to increase the sealing performance and cooling effect during the membrane transfer process.

7. A membrane transfer cassette for Western blotting according to claim 6, wherein, This membrane transfer box is made of a material that is resistant to low temperature, corrosion - resistant and insulating, so as to ensure stability and safety in a low - temperature environment.

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