Oocyte in-vitro operation device

Abstract

The utility model discloses an oocyte in vitro operating device, relates to embryo biotechnology field, including suction bulb tube and jacket, suction bulb tube includes tube body and suction bulb, the suction bulb is provided on the tube body and is communicated with the inside of tube body, the jacket can be fixed cover outside suction bulb tube, the side wall of jacket has first opening, first opening right faces suction bulb, and the suction bulb is provided with second opening right facing suction bulb. And the jacket does not deform after long-time operation. According to the oocyte in-vitro operation device, the control mode that the palm serves as a supporting point and the fingers and the palm cooperate integrally is achieved, the operation stability is greatly improved, meanwhile, the operation durability is remarkably enhanced, and the operation fatigue is reduced.

Description

Technical Field

[0001] The utility model relates to the field of embryo biotechnology, in particular to an in-vitro operation device for oocytes. Background Art

[0002] The development of embryo biotechnology has not only made great contributions to the field of human assisted reproduction, but also played an important role in animal cloning, transgenic breeding and other aspects. There are many technical links in embryo biotechnology, all of which require a tool that can freely suck and release oocytes to complete operations such as cleaning, selecting and transferring oocytes.

[0003] The in-vitro operation of oocytes is a common experimental step. In these operations, the transfer speed and stability of oocytes are crucial for shortening the in-vitro operation time and improving the embryo development efficiency. Usually, these operations are carried out by directly connecting a suction bulb to a Pasteur pipette for oocyte transfer. However, in long-term or large-scale operations of oocytes, this method may cause operator fatigue and oocyte loss. The existing patent (CN210419911U) discloses a device for sucking oocytes or embryos. By pinching the rubber cap with the thumb and index finger of the right or left hand and assisting with the middle finger for fixation, the tightness of the thumb pinching the rubber cap can be used to perform the sucking operation on oocytes. When operating oocytes in the above patent for a long time or in large quantities, it may cause the operator to only hold the suction bulb with the index finger and thumb during operation, lacking stability, and there is no support point on the palm, resulting in unstable operation and finger fatigue, leading to the loss of oocytes during the operation process. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an in-vitro operation device for oocytes to solve the problems existing in the above-mentioned prior art, improve the operation stability and reduce the operation fatigue.

[0005] To achieve the above purpose, the utility model provides the following scheme:

[0006] The utility model provides an in-vitro operation device for oocytes, which includes a suction bulb tube and a jacket. The suction bulb tube includes a tube body and a suction bulb. The suction bulb is arranged on the tube body and is in internal communication with the tube body. The jacket can be fixedly sleeved outside the suction bulb tube. The side wall of the jacket has a first opening, and the first opening is directly opposite to the suction bulb. The jacket will not deform during long-term operation.

[0007] Preferably, the end face of the first end of the outer sleeve is closed, and the end face of the second end of the outer sleeve has a second opening. The second opening faces the pipe orifice of the suction bulb tube near the second opening and can intercept the suction bulb tube. The length of the outer sleeve is the same as that of the suction bulb tube. One end face of the suction bulb tube away from the second opening contacts the end face of the first end, and the other end face of the suction bulb tube contacts the end face of the second end to fix the suction bulb tube.

[0008] Preferably, the distance from the suction bulb to the second opening is less than the distance from the suction bulb to the end face of the first end.

[0009] Preferably, the suction bulb does not protrude from the surface of the outer sleeve.

[0010] Preferably, the pipe body includes a first pipe body and a second pipe body. The first pipe body, the suction bulb, and the second pipe body are detachably connected and communicated in sequence.

[0011] Preferably, the outer sleeve includes a first outer sleeve and a second outer sleeve. The first outer sleeve and the second outer sleeve are detachably connected and communicated, and the first opening is arranged on the side wall of the first outer sleeve.

[0012] Preferably, the suction bulb tube is a hollow columnar body, and one end of the suction bulb tube away from the second opening is closed.

[0013] Preferably, one end of the suction bulb tube contacting the second opening is connected to a Pasteur pipette, and the outer diameter of the Pasteur pipette is equal to the diameter of the second opening.

[0014] Preferably, the Pasteur pipette is a hollow columnar body, the material of the Pasteur pipette is silicon dioxide, and the outer sleeve and the suction bulb tube are both made of sterile PVC material.

[0015] The present utility model has achieved the following technical effects compared with the prior art:

[0016] The present utility model provides an in vitro oocyte manipulation device. The outer sleeve is covered outside the suction bulb tube. The first opening on the outer sleeve is directly opposite to the suction bulb in the pipe body of the suction bulb tube. By pressing the suction bulb at the first opening of the outer sleeve, the transfer of cells can be completed. The outer sleeve is made of hard material and will not deform during long-term operation, which is convenient for operation. Moreover, it can separate the suction bulb from the palm to avoid misoperation. The device can be operated with a skilled movement similar to daily writing, realizing a control mode with the palm as the support point and the overall cooperation of fingers and palm, rather than simply relying on the independent work of the thumb and index finger. This not only greatly improves the operation stability but also significantly enhances the operation durability and reduces operation fatigue. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0018] Figure 1 It is a schematic structural diagram of an in vitro operation device for oocytes in an embodiment of the present invention;

[0019] Wherein: 1 - outer sleeve; 2 - suction bulb; 3 - suction bulb tube; 4 - Pasteur pipette; 5 - first opening; 6 - second opening. Specific embodiments

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0021] To make the above objects, features, and advantages of the present invention more obvious and understandable, the present invention will be further described in detail below with reference to the drawings and specific embodiments.

[0022] The present invention provides an in vitro operation device for oocytes, as Figure 1 shown, which includes a suction bulb tube 3 and an outer sleeve 1. The suction bulb tube 3 includes a tube body and a suction bulb 2. The suction bulb 2 is arranged on the tube body and is in communication with the inside of the tube body. The outer sleeve 1 can be fixedly sleeved outside the suction bulb tube 3. The side wall of the outer sleeve 1 has a first opening 5, and the first opening 5 is directly opposite to the suction bulb 2. The outer sleeve 1 will not deform during long-term operation. By pressing the suction bulb 2 at the first opening 5 of the outer sleeve 1, the transfer of cells can be completed. The outer sleeve 1 is made of a rigid material, such as rigid PVC material, polypropylene material, etc., but not limited thereto. It will not deform during long-term operation, is convenient for operation, and can separate the suction bulb from the palm to avoid misoperation. The in vitro operation device for cells provided by the present invention is particularly suitable for oocytes or embryonic cells, but not limited thereto. It can also be used for the in vitro operation of other types of cells; this device operates with a skilled movement similar to daily writing, realizing a control mode with the palm as the support point and the overall cooperation of the fingers and the palm, rather than simply relying on the independent work of the thumb and index finger, improving the stability of the operation, and at the same time significantly enhancing the durability of the operation and reducing operation fatigue.

[0023] In a more preferred embodiment of the present utility model, the end face of the first end of the outer sleeve 1 is closed, and there is a second opening 6 at the end face of the second end of the outer sleeve 1. The second opening 6 faces the pipe orifice of the pipette bulb tube 3 close to the second opening 6 and can intercept the pipette bulb tube 3. The length of the outer sleeve 1 is the same as the length of the pipette bulb tube 3. The pipette bulb tube 3 is fixed inside the outer sleeve 1, which can enable the in vitro operation experiment of cells to proceed normally without affecting the experiment.

[0024] In a more preferred embodiment of the present utility model, one end face of the pipette bulb tube 3 away from the second opening 6 contacts the end face of the first end, and the other end face of the pipette bulb tube 3 contacts the end face of the second end to fix the pipette bulb tube 3. This can prevent the up and down movement of the pipette bulb tube 3 and improve the accuracy of experimental operations.

[0025] In a more preferred embodiment of the present utility model, the distance from the pipette bulb 2 to the second opening 6 is less than the distance from the pipette bulb 2 to the end face of the first end. The position of the pipette bulb 2 is at the lower middle of the pipette bulb tube 3, which is more convenient for the operator to operate with a skilled movement similar to daily writing, and realize the pen-holding type of pressing the pen core pipette bulb for the in vitro operation of cells.

[0026] In a more preferred embodiment of the present utility model, the pipette bulb 2 does not protrude from the surface of the outer sleeve 1. This can effectively avoid unnecessary operations caused by accidentally touching the pipette bulb 2 and affecting the experimental results.

[0027] In a more preferred embodiment of the present utility model, the tube body includes a first tube body and a second tube body. The first tube body, the pipette bulb 2, and the second tube body are detachably connected and communicated in sequence. The first tube body, the pipette bulb 2, and the second tube body can be disassembled and cleaned separately, which is convenient for cleaning the tube body and the pipette bulb.

[0028] In a more preferred embodiment of the present utility model, the outer sleeve 1 includes a first outer sleeve and a second outer sleeve. The first outer sleeve and the second outer sleeve are detachably connected and communicated, and the first opening 5 is arranged on the side wall of the first outer sleeve; the first outer sleeve and the second outer sleeve are detachably connected, which is convenient for the pipette bulb tube 3 to be inserted into the outer sleeve 1 or taken out from the outer sleeve 1.

[0029] In a more preferred embodiment of the present utility model, the pipette bulb tube 3 is a hollow columnar body, and one end of the pipette bulb tube 3 away from the second opening 6 is closed. After the pipette bulb tube 3 sucks in cells, a closed space is formed inside the pipette bulb tube 3, so that the cells will not flow out of the pipette bulb tube 3, thus completing the in vitro operation of cells.

[0030] In a more preferred embodiment of the present utility model, one end of the pipette bulb tube 3 contacting the second opening 6 is connected to the Pasteur pipette 4, and the outer diameter of the Pasteur pipette 4 is equal to the diameter of the second opening 6. Pressing the pipette bulb 2 can enable the Pasteur pipette to suck in cells and complete the in vitro operation of cells.

[0031] In a more preferred embodiment of the present utility model, the Pasteur pipette 4 is a hollow columnar body, the material of the Pasteur pipette 4 is silica, and both the outer sleeve 1 and the pipette bulb 3 are made of sterile PVC material. Silica has a certain elasticity and high stability, which can enable the Pasteur pipette 4 to be inserted into the pipette bulb 3 and keep the Pasteur pipette 4 durable and unchanged in shape; the outer sleeve 1 is made of rigid PVC material so that the outer sleeve 1 will not deform during long-term operation, and the pipette bulb 3 is made of soft PVC material, which can enable the Pasteur pipette 4 to be firmly inserted into the pipette bulb 3, and the sterile PVC material can effectively prevent the penetration and attachment of microorganisms, bacteria and other pollutants, thereby providing a clean and sterile working environment for in vitro cell manipulation.

[0032] Specific examples are applied in the present utility model to elaborate on the principle and implementation manner of the present utility model. The description of the above embodiments is only used to help understand the method and its core idea of the present utility model; at the same time, for those of ordinary skill in the art, according to the idea of the present utility model, there will be changes in the specific implementation manner and application scope. In summary, the content of this specification should not be construed as a limitation to the present utility model.

Claims

1. An in vitro manipulation device for oocytes, characterized in that: It includes a suction bulb tube and an outer sleeve. The suction bulb tube includes a tube body and a suction bulb. The suction bulb is arranged on the tube body and is in communication with the inside of the tube body. The outer sleeve can be fixedly covered outside the suction bulb tube. The side wall of the outer sleeve has a first opening, and the first opening faces the suction bulb directly. The outer sleeve is made of a rigid material.

2. The oocyte in vitro manipulation device according to claim 1, characterized in that: The end face of the first end of the outer sleeve is closed, and the end face of the second end of the outer sleeve has a second opening. The second opening is opposite to the tube opening of the suction bulb tube close to the second opening and can intercept the suction bulb tube. The length of the outer sleeve is the same as the length of the suction bulb tube. One end face of the suction bulb tube far from the second opening contacts the end face of the first end, and the other end face of the suction bulb tube contacts the end face of the second end to fix the suction bulb tube.

3. The oocyte in vitro manipulation device according to claim 2, characterized in that: The distance from the suction bulb to the second opening is less than the distance from the suction bulb to the end face of the first end.

4. The oocyte in vitro manipulation device according to claim 1, wherein: The suction bulb does not protrude from the surface of the outer sleeve.

5. The oocyte in vitro manipulation device according to claim 1, characterized in that: The tube body includes a first tube body and a second tube body. The first tube body, the suction bulb, and the second tube body are detachably connected and communicated in sequence.

6. The oocyte in vitro manipulation device according to claim 1, characterized in that: The outer sleeve includes a first outer sleeve and a second outer sleeve. The first outer sleeve and the second outer sleeve are detachably connected and communicated, and the first opening is arranged on the side wall of the first outer sleeve.

7. The oocyte in vitro manipulation device according to claim 2, characterized in that: The suction bulb tube is a hollow columnar body, and one end of the suction bulb tube far from the second opening is closed.

8. The oocyte in vitro manipulation device according to claim 7, characterized in that: One end of the suction bulb tube contacting the second opening is connected to a Pasteur tube, and the outer diameter of the Pasteur tube is equal to the diameter of the second opening.

9. The oocyte in vitro manipulation device according to claim 8, wherein: The Pasteur tube is a hollow columnar body, the material of the Pasteur tube is silica, and the outer sleeve and the suction bulb tube are both made of sterile PVC material.

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