Device for measuring free radical scavenging effect of magnolia sieboldii essential oil

Abstract

The utility model discloses a measuring device for a free radical scavenging effect of magnolia sieboldii essential oil, and belongs to the technical field of essential oil measurement. Comprising a bottom plate, a supporting frame is fixedly connected to the top of the bottom plate, and a supporting disc is fixedly connected to the top of the supporting frame; and the rotating assembly comprises a motor fixedly connected into the supporting disc, the top of the motor is fixedly connected with a first rotating shaft, and the top of the first rotating shaft is fixedly connected with a first gear. According to the utility model, the motor, the clamping plate and the cuvette are arranged, and the motor drives the first rotating shaft and the first gear to synchronously rotate, so that the essential oil and the free radical reagent can be fully mixed to react, a plurality of groups of data can be simultaneously measured, and then the free radical clearance rate of the magnolia sieboldii essential oil can be respectively calculated; according to the device, the measuring efficiency is high, meanwhile, errors are avoided, and the accuracy of the measuring result of the device is further guaranteed.

Description

Technical Field

[0001] The utility model belongs to the technical field of essential oil determination, and particularly relates to a device for determining the free radical scavenging effect of Magnolia sieboldii essential oil. Background Technique

[0002] The device for determining the free radical scavenging effect of Magnolia sieboldii essential oil is a device used in the laboratory to test the ability of Magnolia sieboldii essential oil to scavenge free radicals. Free radicals are unstable molecules in living organisms, which can cause cell damage and diseases. Therefore, substances with free radical scavenging ability have important applications in the fields of health care and medicine;

[0003] Some utility model patents in the technical field of essential oil determination are disclosed in the prior art. Among them, the utility model patent with the application number CN219518847U discloses a solution reaction box for determining the DPPH free radical scavenging efficiency, including a reaction box body. A device frame is installed at the top of the reaction box body, and conduits are symmetrically installed at the top of the reaction box body. Rubber blocks are arranged at the tops of the two conduits. A stirring component is arranged inside the reaction box body. The stirring component includes a driving motor, a connecting rod, a sleeve, a first stirring blade, and a second stirring blade. In this utility model, through the arranged stirring component, the reagent is injected into the inside of the liquid storage barrel through the conduit. The driving motor runs to drive the connecting rod to rotate. The first bevel gear on the connecting rod drives the second bevel gear to rotate. When the second bevel gear rotates, it drives the third bevel gear to rotate. The third bevel gear drives the sleeve to rotate outside the connecting rod. The sleeve and the connecting rod rotate in different directions. The first stirring blade and the second stirring blade rotate in different directions, stirring and mixing the reagent inside the liquid storage barrel, making the stirring more uniform and rapid.

[0004] The disadvantages of the prior art are that when determining the free radical scavenging effect of Magnolia sieboldii essential oil, generally, multiple repeated experiments are required for measurement. However, the existing device can only measure reagents of one concentration at the same time. While the measurement efficiency is low, errors are also prone to occur, resulting in inaccurate results.

[0005] Based on this, the utility model designs a device for determining the free radical scavenging effect of Magnolia sieboldii essential oil to solve the above problems. Summary of the Utility Model

[0006] The purpose of the utility model is to provide a device for determining the free radical scavenging effect of Magnolia sieboldii essential oil to solve the problem that the existing device can only measure reagents of one concentration at the same time, with low measurement efficiency and prone to errors, resulting in inaccurate results.

[0007] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0008] An apparatus for measuring the free radical scavenging effect of Magnolia sieboldii K. Koch essential oil, comprising a bottom plate, wherein a support frame is fixedly connected to the top of the bottom plate, and a support disc is fixedly connected to the top of the support frame;

[0009] A rotating assembly, the rotating assembly includes a motor fixedly connected in the support disc, a first rotating shaft is fixedly connected to the top of the motor, a first gear is fixedly connected to the top of the first rotating shaft, six second gears are externally engaged with the first gear, and a second rotating shaft is fixedly connected in each of the six second gears;

[0010] A fixing assembly, the fixing assembly includes a fixing disc fixedly connected to the top of the second rotating shaft, a fixing frame is fixedly connected to the top of the fixing disc, electric push rods are fixedly connected to both the left and right sides of the fixing frame, and clamping plates are fixedly connected to the ends of the two electric push rods close to each other.

[0011] As a further description of the above technical solution:

[0012] The same colorimetric dish is abutted inside the two clamping plates, and a dish cover is hinged to the top of the colorimetric dish.

[0013] As a further description of the above technical solution:

[0014] A handle is arranged outside the dish cover, and a spring is fixedly connected to the top of the fixing disc.

[0015] As a further description of the above technical solution:

[0016] The top of the spring is fixedly connected to a buffer plate, and the buffer plate abuts against the bottom of the colorimetric dish.

[0017] As a further description of the above technical solution:

[0018] All six second rotating shafts are rotatably connected in the support disc, and the support disc is circular in shape.

[0019] As a further description of the above technical solution:

[0020] Friction pads are arranged inside the clamping plates, and the clamping plates are arc-shaped.

[0021] As a further description of the above technical solution:

[0022] The six second gears are evenly distributed outside the first gear, and the fixing frame is in a hollow shape.

[0023] As a further description of the above technical solution:

[0024] The buffer plate is concave in shape and fits the bottom of the colorimetric dish, and the outer surface of the colorimetric dish is transparent.

[0025] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present utility model are as follows:

[0026] 1. In the present utility model, by setting a motor, a second gear, a fixing bracket, an electric push rod, a clamping plate and a colorimetric cuvette, the motor drives the first rotating shaft and the first gear to rotate synchronously, thereby driving six second gears, second rotating shafts, a fixing disk, a fixing bracket, an electric push rod, a clamping plate and a colorimetric cuvette to rotate synchronously, enabling the device to fully mix and react the essential oil with the free radical reagent, and capable of measuring multiple groups of data simultaneously, then respectively calculating the scavenging rate of Magnolia sieboldii essential oil on free radicals, and finally calculating the average value, which not only has high measurement efficiency but also avoids easy occurrence of errors, further ensuring the accuracy of the measurement results of the device.

[0027] 2. In the present utility model, by setting a colorimetric cuvette, an electric push rod, a clamping plate, a spring and a buffer plate, the colorimetric cuvette is attached to the buffer plate, and the buffer plate can buffer the buffer plate under the action of the self-elastic force of the spring, and the electric push rod can push the two clamping plates to limit the two sides of the colorimetric cuvette, not only ensuring the stability of the colorimetric cuvette during rotation but also avoiding the situation that it is easily damaged due to shaking. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a three-dimensional structural schematic diagram of a device for measuring the free radical scavenging effect of Magnolia sieboldii essential oil proposed by the present utility model;

[0029] Figure 2 is a three-dimensional structural schematic diagram of the colorimetric cuvette of a device for measuring the free radical scavenging effect of Magnolia sieboldii essential oil proposed by the present utility model;

[0030] Figure 3 is a three-dimensional structural schematic diagram of the fixing disk of a device for measuring the free radical scavenging effect of Magnolia sieboldii essential oil proposed by the present utility model;

[0031] Figure 4 is a three-dimensional structural schematic diagram of the electric push rod of a device for measuring the free radical scavenging effect of Magnolia sieboldii essential oil proposed by the present utility model;

[0032] Figure 5 is a three-dimensional structural schematic diagram of the fixing bracket of a device for measuring the free radical scavenging effect of Magnolia sieboldii essential oil proposed by the present utility model.

[0033] Legend Explanation:

[0034] 1. Base plate; 2. Support frame; 3. Support disc; 4. Motor; 5. First rotating shaft; 6. First gear; 7. Second gear; 8. Second rotating shaft; 9. Fixed disc; 10. Fixed frame; 11. Electric push rod; 12. Clamping plate; 13. Colorimetric cuvette; 14. Cuvette cover; 15. Handle; 16. Spring; 17. Buffer plate. Detailed implementation manner

[0035] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0036] Please refer to Figures 1-5 , the first embodiment:

[0037] The present invention provides a technical solution:

[0038] A device for measuring the free radical scavenging effect of Magnolia sieboldii essential oil, comprising a base plate 1, a support frame 2 fixedly connected to the top of the base plate 1, and a support disc 3 fixedly connected to the top of the support frame 2;

[0039] A rotating assembly, the rotating assembly includes a motor 4 fixedly connected inside the support disc 3, a first rotating shaft 5 fixedly connected to the top of the motor 4, a first gear 6 fixedly connected to the top of the first rotating shaft 5, and six second gears 7 externally meshed with the first gear 6, and a second rotating shaft 8 is fixedly connected inside each of the six second gears 7;

[0040] A fixing assembly, the fixing assembly includes a fixed disc 9 fixedly connected to the top of the second rotating shaft 8, a fixed frame 10 fixedly connected to the top of the fixed disc 9, electric push rods 11 fixedly connected to both the left and right sides of the fixed frame 10, and clamping plates 12 fixedly connected to the ends of the two electric push rods 11 close to each other.

[0041] All six second rotating shafts 8 are rotatably connected inside the support disc 3, the support disc 3 is circular in shape, a friction pad is provided inside the clamping plate 12, the clamping plate 12 is arc-shaped, the six second gears 7 are evenly distributed outside the first gear 6, the fixed frame 10 is in a hollow shape, the buffer plate 17 is concave and fits the bottom of the colorimetric cuvette 13, and the outer surface of the colorimetric cuvette 13 is transparent.

[0042] By setting up the motor 4, the second gear 7, the fixing bracket 10, the electric push rod 11, the clamping plate 12 and the colorimetric cuvette 13, the device can enable the essential oil to fully mix and react with the free radical reagent, and can measure multiple groups of data simultaneously. Then, the scavenging rate of the magnolia sieboldii essential oil on free radicals is calculated respectively, and finally the average value is calculated. While the measurement efficiency is high, it also avoids easy occurrence of errors, further ensuring the accuracy of the measurement results of the device.

[0043] Second Embodiment:

[0044] Specifically, as Figures 2-4 shown, the same colorimetric cuvette 13 is abutted inside two clamping plates 12. The top of the colorimetric cuvette 13 is hinged with a cuvette cover 14, and a handle 15 is arranged outside the cuvette cover 14. The top of the fixed disk 9 is fixedly connected with a spring 16, and the top of the spring 16 is fixedly connected with a buffer plate 17, and the buffer plate 17 abuts against the bottom of the colorimetric cuvette 13.

[0045] By setting up the colorimetric cuvette 13, the electric push rod 11, the clamping plate 12, the spring 16 and the buffer plate 17, not only the stability of the colorimetric cuvette 13 during rotation is ensured, but also the situation that it is easily damaged due to shaking is avoided.

[0046] Working principle: When in use, first use an external pipette to accurately measure a certain concentration of free radical reagent. After opening the cuvette cover 14, put it into different colorimetric cuvettes 13 respectively. Then place the colorimetric cuvettes 13 in the corresponding fixing brackets 10 and fit them with the buffer plate 17, so that the spring 16 is in a compressed state. Then start the corresponding two electric push rods 11, which can push the two clamping plates 12 to limit the two sides of the colorimetric cuvette 13. Then add the magnolia sieboldii essential oil into the free radical reagent in the colorimetric cuvette 13 according to the same certain proportion respectively. Then start the motor 4, and the motor 4 drives the first rotating shaft 5 and the first gear 6 to rotate synchronously, thereby driving six second gears 7, second rotating shafts 8, fixed disks 9 and fixing brackets 10 to rotate synchronously, and driving the corresponding electric push rods 11, clamping plates 12 and colorimetric cuvettes 13 to rotate continuously, so that the essential oil and the free radical reagent can fully mix and react, and make the colorimetric cuvette 13 in a centrifugal state. After a short time, turn off the motor 4, open the cuvette cover 14, and then use an external simple photometer to measure the absorbance of the supernatant after centrifugation. According to the change of the absorbance, calculate the scavenging rate of the magnolia sieboldii essential oil on free radicals respectively, and record the data and calculate the average value.

[0047] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and the inventive concept of the present invention, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present invention.

Claims

1. An apparatus for measuring the free radical scavenging effect of Magnolia sieboldii K. Koch essential oil, comprising a bottom plate (1), characterized in that, The top of the bottom plate (1) is fixedly connected with a support frame (2), and the top of the support frame (2) is fixedly connected with a support disc (3); A rotating assembly, the rotating assembly includes a motor (4) fixedly connected in the support disc (3), the top of the motor (4) is fixedly connected with a first rotating shaft (5), the top of the first rotating shaft (5) is fixedly connected with a first gear (6), and six second gears (7) are externally engaged with the first gear (6), and a second rotating shaft (8) is fixedly connected in each of the six second gears (7); A fixing assembly, the fixing assembly includes a fixing disc (9) fixedly connected to the top of the second rotating shaft (8), a fixing frame (10) is fixedly connected to the top of the fixing disc (9), electric push rods (11) are fixedly connected to both the left and right sides of the fixing frame (10), and clamping plates (12) are fixedly connected to the ends of the two electric push rods (11) close to each other.

2. The device for measuring the free radical scavenging effect of Magnolia sieboldii K. Koch essential oil according to claim 1, wherein, A same colorimetric cuvette (13) is abutted inside the two clamping plates (12), and a cuvette cover (14) is hinged to the top of the colorimetric cuvette (13).

3. An apparatus for measuring the free radical scavenging effect of magnolia sieboldii oil according to claim 2, wherein, A handle (15) is arranged outside the cuvette cover (14), and a spring (16) is fixedly connected to the top of the fixing disc (9).

4. The determination device for the free radical scavenging effect of Magnolia sieboldii K. Koch essential oil according to claim 3, wherein, The top of the spring (16) is fixedly connected with a buffer plate (17), and the buffer plate (17) abuts against the bottom of the colorimetric cuvette (13).

5. The determination device for the free radical scavenging effect of magnolia sieboldii oil according to claim 1, characterized in that, All six second rotating shafts (8) are rotatably connected in the support disc (3), and the support disc (3) is circular in shape.

6. The apparatus for measuring the free radical scavenging effect of Magnolia sieboldii K. Koch essential oil according to claim 1, wherein A friction pad is arranged inside the clamping plate (12), and the clamping plate (12) is arc-shaped.

7. The apparatus for measuring the free radical scavenging effect of magnolia sieboldii oil according to claim 1, wherein, The six second gears (7) are evenly distributed outside the first gear (6), and the fixing frame (10) is in a hollow shape.

8. The determination device for the free radical scavenging effect of Magnolia sieboldii K. Koch essential oil according to claim 4, characterized in that, The buffer plate (17) is concave in shape and fits the bottom of the colorimetric cuvette (13), and the outer surface of the colorimetric cuvette (13) is transparent.

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