Cell protein extraction device

Abstract

The utility model relates to the field of cell protein extraction and discloses a cell protein extraction device which comprises an ice storage box, a fixing plate is fixedly mounted at the top in the ice storage box, a plurality of first fixing holes are formed in the upper surface of the fixing plate in a penetrating mode, and a plurality of ice baffles are welded to the positions, close to the outer surfaces of the first fixing holes, of the bottom end of the fixing plate. Supporting columns are fixedly installed at the positions, close to the four corners, of the inner wall of the ice storage box, movable plates are movably installed on the outer surfaces of the supporting columns, a plurality of second fixing holes are formed in the positions, corresponding to the first fixing holes, of the upper surfaces of the movable plates in a penetrating mode, and EP pipes are placed in the second fixing holes; the movable plate is matched with the fixed plate and the ice baffle to support the EP pipe, so that the effect of keeping the EP pipe stably placed in the crushed ice is achieved, the overall low-temperature cooling effect of the EP pipe is improved, the problem that the EP pipe inclines when being inserted into the crushed ice is solved, and the overall experimental efficiency of cell protein extraction is improved.

Description

Technical Field

[0001] The present application relates to the field of cell protein extraction, and particularly to a cell protein extraction device. Background Art

[0002] Cell protein extraction is a common operation step in the laboratory, used to extract proteins from cells for subsequent analysis, purification, or detection. During the experiment, first, the EP tube containing the sample needs to be pre-cooled to facilitate the preservation of cell proteins, and a lysis buffer containing an enzyme inhibitor is added to the sample during the experiment, usually used to protect proteins, nucleic acids, or other biomolecules in the sample from degradation by endogenous enzymes. The lysed sample is centrifuged to enrich the proteins in the supernatant, and then the supernatant is extracted into a new centrifuge tube.

[0003] Since during the experiment, the EP tube containing the sample needs to be cooled at low temperature multiple times. Generally, a certain amount of crushed ice is placed in a box body, and then the EP tube containing the sample is inserted into the crushed ice. However, the stability of the EP tube when placed in the crushed ice is insufficient, and the problem of tilting and toppling may occur. The tilted EP tube is not convenient for the experimenter to pick up and place, which has a certain impact on the experiment operation.

[0004] Regarding the above related technologies, the inventor believes that there is a defect that the EP tube is tilted when inserted into the crushed ice during the cooling of the EP tube in the cell protein extraction experiment. Therefore, a cell protein extraction device is proposed to solve the above problems. Utility Model Content

[0005] In order to solve the problem that the EP tube is tilted when inserted into the crushed ice during the cooling of the EP tube in the cell protein extraction experiment, the present application provides a cell protein extraction device.

[0006] The cell protein extraction device provided by the present application adopts the following technical solution:

[0007] A cell protein extraction device includes a refrigerator. A fixing plate is fixedly installed on the inner top of the refrigerator. A plurality of first fixing holes are formed through the upper surface of the fixing plate. A plurality of ice blocking plates are welded to the outer surface of the bottom end of the fixing plate near the first fixing holes. Support columns are fixedly installed at the four corners of the inner wall of the refrigerator. A movable plate is movably installed on the outer surface of the support columns. A plurality of second fixing holes are formed through the upper surface of the movable plate at positions corresponding to the first fixing holes. EP tubes are placed inside the second fixing holes. Fixing frames are fixedly installed on both side edges of the top end of the movable plate. A fixing groove is formed on the upper surface of the fixing frame. A limiting mechanism is further provided inside the fixing groove for fixing the movable plate at a specific position on the outer surface of the support column.

[0008] Preferably, the limiting mechanism includes a movable block, the movable block is symmetrically and movably installed inside the fixed groove, a spring is installed between the two movable blocks, one side of the movable block passes through the outside of the fixed frame and is welded with a limiting rod, the top edge of the movable block extends above the fixed groove and is fixedly connected with a fixed connecting rod, the outer surfaces of the two fixed connecting rods on the same side are sleeved with movable sleeve plates at the top, and a limiting hole is penetrated and opened at the top of one side of the outer surface of the support column.

[0009] Preferably, the cross-section of the ice baffle is an L-shaped structure, the outer diameter of the EP tube is smaller than the inner diameters of the second fixing hole and the first fixing hole, and the bottom end of the EP tube passes through the movable plate and the fixing plate and overlaps on the bottom end of the inner wall of the ice baffle.

[0010] Preferably, through holes are penetrated and opened at the four corners of the upper surface of the movable plate, the outer diameter of the support column is smaller than the inner diameter of the through hole, and one side of the outer surface of the support column is located inside the through hole.

[0011] Preferably, circular holes are penetrated and opened at the front and rear ends of the inner wall of the fixed groove, the outer diameter of the limiting rod is smaller than the inner diameters of the limiting hole and the circular hole, one end of the limiting rod passes through the circular hole and is placed inside the limiting hole, and the limiting rod and the limiting hole are mutually engaged.

[0012] In summary, the present application includes the following beneficial technical effects:

[0013] By pulling the fixed connecting rod to contract the limiting rod into the fixed frame, the movable plate is lifted to a certain height, the limiting rod is reset and movably limited into the limiting hole, and then the movable plate is fixed. The EP tube containing the sample is placed in the second fixing hole, and at the same time, broken ice is added to the refrigerator, so that the limiting rod cancels the limit on the movable plate, and the movable plate drives the EP tube to slide down through the fixing plate and be placed in the ice baffle; compared with the prior art, this solution has the effect of facilitating the stable placement of the EP tube in the broken ice, improving the overall low-temperature cooling effect of the EP tube, solving the problem of the EP tube being skewed when inserted into the broken ice for placement, and improving the overall experimental efficiency of cell protein extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is the overall structural schematic diagram of the application embodiment;

[0015] Figure 2 is the cross-sectional structural schematic diagram of the movable plate in the application embodiment;

[0016] Figure 3 is the cross-sectional structural schematic diagram of the fixed frame in the application embodiment;

[0017] Figure 4 is the application embodiment Figure 3 is the partial enlarged structural schematic diagram of A in the middle;

[0018] Description of the reference numerals: 1, refrigerator; 2, fixed plate; 3, first fixing hole; 4, ice baffle; 5, support column; 6, movable plate; 7, second fixing hole; 8, EP tube; 9, fixing bracket; 10, fixing groove; 11, movable block; 12, spring; 13, limiting rod; 14, fixed connecting rod; 15, movable sleeve plate; 16, limiting hole. Specific embodiments

[0019] The following will further describe the present application in detail with reference to the Figures 1 - 4 accompanying drawings.

[0020] An embodiment of the present application discloses a cell protein extraction device. Refer to Figures 1 - 4 FIG., a cell protein extraction device, including a refrigerator 1, a fixed plate 2 is fixedly installed on the inner top of the refrigerator 1, a plurality of first fixing holes 3 are penetrated and opened on the upper surface of the fixed plate 2, a plurality of ice baffles 4 are welded at the outer surface of the bottom end of the fixed plate 2 near the first fixing holes 3, support columns 5 are fixedly installed at the inner wall of the refrigerator 1 near the four corners, a movable plate 6 is movably installed on the outer surface of the support column 5, through holes are penetrated and opened at the four corners of the upper surface of the movable plate 6, the outer diameter of the support column 5 is smaller than the inner diameter of the through hole, one side of the outer surface of the support column 5 is located inside the through hole, a plurality of second fixing holes 7 are penetrated and opened at the corresponding positions of the upper surface of the movable plate 6 and the first fixing holes 3, an EP tube 8 is placed inside the second fixing hole 7, the cross section of the ice baffle 4 is an L-shaped structure, the outer diameter of the EP tube 8 is smaller than the inner diameters of the second fixing hole 7 and the first fixing hole 3, the bottom end of the EP tube 8 passes through the movable plate 6 and the fixed plate 2 and is lapped on the bottom end of the inner wall of the ice baffle 4, fixing brackets 9 are fixedly installed on both side edges of the top end of the movable plate 6, a fixing groove 10 is opened on the upper surface of the fixing bracket 9, and a limiting mechanism is further provided inside the fixing groove 10 for fixing the movable plate 6 at a specific position on the outer surface of the support column 5.

[0021] By pulling the movable sleeve plate 15 to lift the movable plate 6 along the outer surface of the support column 5 to a certain height, a plurality of EP tubes 8 containing samples are placed in the second fixing holes 7. At the same time, the refrigerator 1 is filled with crushed ice, so that the movable plate 6 drives the EP tube 8 to move downward, and the EP tube 8 slides down through the fixed plate 2 and is placed in the ice baffle 4, so that the debris stored on the outer surface of the ice baffle 4 cools the EP tube 8, reflecting the limiting and supporting effect of the ice baffle 4 on the EP tube 8 in the crushed ice.

[0022] Refer to Figure 3 and Figure 4, the limiting mechanism includes a movable block 11, the movable block 11 is symmetrically and movably installed inside the fixed groove 10, a spring 12 is installed between the two movable blocks 11, one side of the movable block 11 passes through the outside of the fixed frame 9 and is welded with a limiting rod 13, the top edge of the movable block 11 extends above the fixed groove 10 and is fixedly connected with a fixed connecting rod 14, the outer surfaces of the two fixed connecting rods 14 on the same side are sleeved with movable sleeve plates 15 at the top, a limiting hole 16 is penetrated and opened at the top of one side of the outer surface of the support column 5, round holes are penetrated and opened at both the front and rear ends of the inner wall of the fixed groove 10, the outer diameter of the limiting rod 13 is smaller than the inner diameters of the limiting hole 16 and the round hole, one end of the limiting rod 13 passes through the round hole and is placed inside the limiting hole 16, and the limiting rod 13 and the limiting hole 16 are engaged with each other.

[0023] By pulling the fixed connecting rods 14 to approach each other along the inside of the movable sleeve plate 15, the limiting rod 13 is contracted into the fixed frame 9, the movable block 11 is pushed to compress the spring 12, the movable plate 6 is pulled to lift a certain height along the outer surface of the support column 5, the fixed connecting rods 14 are released, the movable block 11 is reset and moves under the action of the spring 12, the limiting rod 13 moves through the fixed frame 9 and is limited in the limiting hole 16, and then the movable plate 6 is fixed on the outer surface of the support column 5, reflecting the limiting and fixing effect of the movable plate 6 on the outer surface of the support column 5.

[0024] The implementation principle of the cell protein extraction device in the embodiment of the present application is as follows: by pulling the fixed connecting rods 14 to approach each other along the inside of the movable sleeve plate 15, the limiting rod 13 is contracted into the fixed frame 9, the movable block 11 is pushed to compress the spring 12, the movable plate 6 is pulled to lift a certain height along the outer surface of the support column 5, the fixed connecting rods 14 are released, the movable block 11 is reset and moves under the action of the spring 12, the limiting rod 13 moves through the fixed frame 9 and is limited in the limiting hole 16, and then the movable plate 6 is fixed on the outer surface of the support column 5. A plurality of EP tubes 8 containing samples are placed in the second fixed holes 7, and at the same time, the storage refrigerator 1 is filled with crushed ice. The fixed connecting rods 14 are pulled again to cancel the limitation of the limiting rod 13 on the movable plate 6, the movable plate 6 drives the EP tubes 8 to move downward, the EP tubes 8 slide down through the fixing plate 2 and are placed in the ice baffle 4, and the debris stored on the outer surface of the ice baffle 4 cools the EP tubes 8; compared with the prior art, this solution has the effect of facilitating the stable placement of the EP tubes 8 in the crushed ice, improving the overall low-temperature cooling effect of the EP tubes 8, solving the problem of the EP tubes 8 being skewed when inserted into the crushed ice, and improving the overall experimental efficiency of cell protein extraction.

[0025] The following points should be noted: First, in the description of this application, it should be noted that unless otherwise specified and defined, the terms "installed", "connected", and "coupled" should be understood in a broad sense. It can be a mechanical connection or an electrical connection, or it can be the internal communication of two components. It can be directly connected. "Upper", "lower", "left", "right", etc. are only used to represent relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may change;

[0026] Second, in the drawings of the disclosed embodiments of the present utility model, only the structures related to the disclosed embodiments are involved. For other structures, reference can be made to the usual designs. Without conflict, the same embodiment and different embodiments of the present utility model can be combined with each other;

[0027] Finally, the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.

[0028] The above are all the preferred embodiments of this application and do not limit the protection scope of this application accordingly. Therefore, all equivalent changes made according to the structure, shape, and principle of this application shall be covered by the protection scope of this application.

Claims

1. A cell protein extraction device, comprising a refrigerator (1), characterized in that: A fixed plate (2) is fixedly installed on the inner top of the refrigerator (1). A number of first fixing holes (3) are penetrated and opened on the upper surface of the fixed plate (2). A number of ice baffle plates (4) are welded to the outer surface of the bottom end of the fixed plate (2) near the first fixing holes (3). Support columns (5) are fixedly installed at the four corners near the inner wall of the refrigerator (1). A movable plate (6) is movably installed on the outer surface of the support column (5). A number of second fixing holes (7) are penetrated and opened at positions corresponding to the first fixing holes (3) on the upper surface of the movable plate (6). An EP tube (8) is placed inside the second fixing holes (7). Fixed frames (9) are fixedly installed on both side edges at the top end of the movable plate (6). A fixing groove (10) is opened on the upper surface of the fixed frame (9). A limiting mechanism is further provided inside the fixing groove (10) for fixing the movable plate (6) at a specific position on the outer surface of the support column (5).

2. The cell protein extraction device according to claim 1, wherein: The limiting mechanism includes movable blocks (11). The movable blocks (11) are symmetrically and movably installed inside the fixing groove (10). A spring (12) is installed between the two movable blocks (11). One side of the movable block (11) passes through the outside of the fixed frame (9) and is welded with a limiting rod (13). The top edge of the movable block (11) extends above the fixing groove (10) and is fixedly connected with a fixed connecting rod (14). Movable sleeve plates (15) are sleeved on the outer surfaces of the two fixed connecting rods (14) on the same side at the top. A limiting hole (16) is penetrated and opened at the top of one side of the outer surface of the support column (5).

3. The cell protein extraction device according to claim 1, wherein: The cross-section of the ice baffle plate (4) is an L-shaped structure. The outer diameter of the EP tube (8) is smaller than the inner diameters of the second fixing holes (7) and the first fixing holes (3). The bottom end of the EP tube (8) passes through the movable plate (6) and the fixed plate (2) and overlaps on the inner bottom end of the ice baffle plate (4).

4. The cell protein extraction device according to claim 1, wherein: Through holes are penetrated and opened at the four corners near the upper surface of the movable plate (6). The outer diameter of the support column (5) is smaller than the inner diameter of the through holes. One side of the outer surface of the support column (5) is located inside the through holes.

5. The cell protein extraction device according to claim 2, wherein: Round holes are penetrated and opened at the front and rear ends of the inner wall of the fixing groove (10). The outer diameter of the limiting rod (13) is smaller than the inner diameters of the limiting hole (16) and the round holes. One end of the limiting rod (13) passes through the round hole and is placed inside the limiting hole (16). The limiting rod (13) and the limiting hole (16) are mutually engaged.

Images