Bursaphelenchus xylophilus multichannel speed-up separation and collection

Abstract

The utility model relates to the technical field of plant quarantine, in particular to a multi-channel speed-up separation and collection device for pine wood nematodes. Comprising an oscillating rotating table and a plurality of separating and collecting units connected to the oscillating rotating table, each separating and collecting unit comprises a sample basket and a nematode collecting pipe, a vertical sample cavity is formed in each sample basket, the upper end of each sample cavity is connected with a sample cavity cover, and the inside and the outside of each sample cavity are connected with an internal heater and an external heater respectively. The bottom of the sample cavity is connected with a nematode collecting pipe and a drain valve, and the bottom of the nematode collecting pipe inclines outwards. The device is reasonable in structure and ingenious in design, pine wood nematodes can be extracted from eight batches of wood samples at the same time, meanwhile, the nematodes in the wood samples can be rapidly extracted within 2 hours, due to the research and development of the device, the nematodes in multiple batches of wood samples can be rapidly and efficiently extracted, the device is simple, convenient and easy to operate, and the device has important significance on rapid customs clearance of customs wood packages.

Description

Technical Field

[0001] The utility model relates to the technical field of plant quarantine, in particular to a multi-channel speed-up separation and collection device for Bursaphelenchus xylophilus. Background Art

[0002] Bursaphelenchus xylophilus can cause pine wilt disease, known as "pine cancer", which is one of the most serious forestry disasters in China. Timely and accurate diagnosis of infected wood, clearing of infected wood in the forest, and prohibiting the circulation of infected wood are still the key links in the prevention and control of this disease. How to quickly detect wooden packages has become an urgent problem to be solved.

[0003] At present, the vast majority of methods for detecting wooden packages at ports are to separate nematodes first and then identify them. When separating nematodes, the wood samples to be tested need to be split into small strips as thick as chopsticks and then placed in containers such as funnels or large beakers. After 12 - 24 hours, a nematode separation solution is obtained. Using the traditional Baermann funnel method for separation takes more than 12 hours. Content of the Utility Model

[0004] The utility model aims at the problems existing in the above-mentioned prior art and provides a multi-channel speed-up separation and collection device for Bursaphelenchus xylophilus.

[0005] The technical solution adopted by the utility model to achieve the above purpose is: a multi-channel speed-up separation and collection device for Bursaphelenchus xylophilus, which is characterized by comprising an oscillating rotating table and a plurality of separation and collection units connected to the table. The separation and collection unit includes a sample basket and a nematode collection tube. The inside of the sample basket is a vertical sample cavity. The upper end of the sample cavity is connected to a sample cavity cover. An internal heater and an external heater are respectively connected inside and outside the sample cavity. The bottom of the sample cavity is respectively connected to a nematode collection tube and a drain valve. The bottom of the nematode collection tube inclines outwards.

[0006] The oscillating rotating table is connected to a main motor.

[0007] The nematode collection tube is detachably connected, the central axis forms an angle of 45 degrees with the horizontal plane, and its maximum volume is 12 ml.

[0008] There are a total of eight separation and collection units, which are arranged in a circle. The oscillating rotating table is located at the center of the circle and is connected to a main motor below. The bottom of the whole device is connected to legs.

[0009] The structure of the utility model is reasonable and ingeniously designed. It can be used to extract Bursaphelenchus xylophilus from eight batches of wood samples at the same time, and can also achieve the rapid extraction of nematodes in the wood samples within 2 hours. The research and development of this device realizes the rapid and efficient extraction of nematodes from multiple batches of wood samples, and is simple and easy to operate. Description of the Drawings

[0010] Figure 1 This is the top view of the utility model (removing the internal and external heaters);

[0011] Figure 2 is Figure 1 the A-A sectional view of;

[0012] In the figure: 1 - sample chamber cover, 2 - external heater, 3 - sample basket, 4 - sample chamber, 5 - internal heater, 6 - oscillating rotary table, 7 - nematode collection tube, 8 - main motor, 9 - drain valve, 10 - leg, α - the angle between the central axis of the nematode collection tube and the horizontal plane. Specific implementation mode Embodiment

[0013] As Figures 1-2 shown in the multi-path speed-up separation and collection device for pine wood nematodes, which includes an oscillating rotary table 6 and a plurality of separation and collection units connected to the table. The separation and collection unit includes a sample basket 3 and a nematode collection tube 7. The inside of the sample basket 3 is a vertical sample chamber 4. The upper end of the sample chamber 4 is connected to a sample chamber cover 1. The inside and outside of the sample chamber 4 are respectively connected to an internal heater 5 and an external heater 2. Both heaters are jointly controlled by a temperature adjustment button. The bottom of the sample chamber 4 is respectively connected to a nematode collection tube 7 and a drain valve 9. The bottom of the nematode collection tube 7 is inclined outwards.

[0014] The oscillating rotary table 6 is connected to a main motor 8, and its rotation centrifugal speed is adjusted and controlled by a centrifugal button. The maximum centrifugal speed is 2000 r / min.

[0015] The nematode collection tube 7 is detachably connected. The angle α between the central axis and the horizontal plane is 45 degrees, and its maximum volume is 12 ml.

[0016] There are a total of eight separation and collection units. The cross-section of each chamber is fan-shaped and arranged in a circle. The oscillating rotary table 6 is located at the center of the circle, and the main motor 8 is connected below. The bottom of the whole device is connected to legs 10.

[0017] The usage method of the utility model: Put the sampled planed wood chips into the sample chamber 4, turn on the temperature adjustment button, and adjust the temperature to 29 - 32 degrees. Both heaters control the temperature simultaneously. Let it stand for 35 min, then oscillate for 10 min, then turn on the centrifugal button, adjust the centrifugal speed to 2000 r / min, and centrifuge for 10 min. At this time, the nematodes are collected into the nematode collection tube 7. Open the drain valve 9 to drain the water in the sample chamber 4, and then unscrew the nematode collection tube 7. At this time, the nematodes are successfully collected.

[0018] This device can simultaneously perform nematode extraction operations on 8 batches of wood samples, greatly improving the nematode separation efficiency.

Claims

1. The multi-channel speed-up separation and collection device for Bursaphelenchus xylophilus is characterized in that It includes an oscillating rotary table and a plurality of separation and collection units connected to the table. The separation and collection unit includes a sample basket and a nematode collection tube. The interior of the sample basket is a vertical sample cavity. The upper end of the sample cavity is connected to a sample cavity cover. An internal heater and an external heater are respectively connected to the inside and outside of the sample cavity. The bottom of the sample cavity is respectively connected to a nematode collection tube and a drain valve. The bottom of the nematode collection tube slopes outward.

2. The pine wood nematode multi-channel speed-up separation and collection device according to claim 1, characterized in that, The oscillating rotary table is connected to a main motor.

3. The pine wood nematode multi-channel speed-up separation and collection device according to claim 1, characterized in that, The nematode collection tube is detachably connected, with the central axis at an angle of 45 degrees to the horizontal plane, and its maximum volume is 12 ml.

4. The pine wood nematode multi-channel speed-up separation and collection device according to any one of claims 1 to 3, characterized in that, There are a total of eight separation and collection units, arranged in a circle. The oscillating rotary table is located at the center of the circle and is connected to the main motor below. The bottom of the whole device is connected to legs.

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