Plate coating device for experiment

Abstract

The utility model discloses a plate coating device for experiments, which comprises a base on which a sample loading unit, a sampling unit and a coating unit are arranged, the sample carrying unit comprises a rotating column, a horizontal carrying plate is fixedly connected to the rotating column, insertion holes are uniformly distributed in the horizontal carrying plate, and liquid storage pipes are arranged in the insertion holes; the sampling unit comprises a lifting column, a rotating seat is arranged at the lifting end of the lifting column, inserting rods are uniformly distributed on the side wall of the rotating seat, sampling rods are inserted into the inserting rods, the outer ends of the sampling rods are vertically bent downwards, and the lower ends of the sampling rods are connected with sampling heads; the coating unit comprises a support, a coating barrel is erected on the support and composed of a cylindrical barrel body and a conical barrel body, an adaptive opening is formed in the middle of the cylindrical barrel body, an air blower is arranged at the top of the cylindrical barrel body, a telescopic rod is arranged on the portion, under the conical barrel body, of the base, and a chuck is arranged at the upper end of the telescopic rod. The device has the advantages that automatic plate coating operation is easily realized, uniform coating of the culture dishes is realized, labor force is reduced, and plate coating efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiments, in particular to a plate coating device for experiments. Background Art

[0002] Plate coating is to evenly coat tiny living organisms such as cells and bacteria into a culture dish, and plate coating operations are often required in biological experiments. For example, during plasmid transformation, the plasmid needs to be mixed with the competent peptide and then coated onto an agarose culture dish with a diameter of 10 cm. Then, after culturing at 37°C for about 12 hours, single Escherichia coli colonies are formed in the culture dish. Finally, one of the colonies is selected for plasmid amplification. To ensure the singularity of subsequent plasmid amplification, the plasmid in the mixture needs to be evenly dispersed and distributed in the culture dish during plate coating, avoiding local aggregation that affects the formation and selection of Escherichia coli colonies. Currently, usually, the plasmid mixture is dropped onto the surfaces of several glass beads, and then the glass beads are shaken in the culture dish so that the plasmid mixture on their surfaces is smeared onto the culture dish. This operation method often cannot ensure that the plasmid mixture is evenly coated in the culture dish. There will be no plasmid in the places where the glass beads do not pass, while there will be an excessive amount of plasmid in the places where the glass beads pass multiple times. Another example is that during cell cloning, the cells need to be coated as evenly as possible to ensure that the cells are singly dispersed into the culture dish, facilitating clear observation of the size of monoclonal formation after about 2 weeks of cell growth. To ensure even coating of the cells, currently, usually, the cells are mixed into the culture medium, then dropped into the culture dish, and the culture dish is rotated for mixing. During the rotation process, the cells often gather in the center of the culture dish and cannot be evenly distributed throughout the culture dish. Summary of the Invention

[0003] The purpose of the utility model is to provide a plate coating device for experiments aiming at the defects of the prior art, which can easily complete the even plate coating of the culture dish.

[0004] To achieve the above purpose, the utility model can adopt the following technical solutions:

[0005] The plate coating device for experiments of the utility model includes a base, on which a sample loading unit, a sampling unit, and a coating unit are arranged;

[0006] Among them, the sample loading unit includes a rotating column vertically arranged on the base, a horizontal loading plate is fixedly connected to the rotating column, a plurality of jacks are evenly distributed along the circumferential direction on the horizontal loading plate, and a liquid storage tube for containing the sample liquid is arranged in the jacks;

[0007] The sampling unit includes a lifting column vertically arranged on the base. A rotating seat that rotates horizontally is arranged at the lifting end of the lifting column. A plurality of insertion rods are circumferentially and uniformly distributed on the side wall of the rotating seat. A sampling rod is inserted into the insertion rod. The outer end of the sampling rod is bent vertically downward, and a sampling head for extending into the liquid storage tube to dip the sample liquid is connected to the lower end thereof.

[0008] The coating unit includes a bracket vertically arranged on the base. A coating cylinder is mounted on the bracket. The coating cylinder is composed of a cylindrical barrel in the upper part and a conical barrel in the lower part. An adaptation port matching the sampling head is opened in the middle of the cylindrical barrel. A hair dryer with a downward air outlet is arranged at the top of the cylindrical barrel. A telescopic rod is vertically arranged on the base directly below the conical barrel. A chuck for horizontally clamping the culture dish to be coated is arranged at the upper end of the telescopic rod.

[0009] Further, the sampling head includes a horizontal ring fixedly connected to the lower end of the sampling rod. An annular upper gasket and an annular lower gasket are clamped in the horizontal ring. A liquid-absorbing sponge pad is clamped between the annular upper gasket and the annular lower gasket. The chuck includes a clamping seat fixedly connected to the upper end of the telescopic rod. A pair of horizontally sliding clamping arms facing each other are connected to the top surface of the clamping seat through springs. Arc-shaped clamping plates for clamping the outer edge of the culture dish to be coated are arranged at the outer ends of the two horizontal clamping arms.

[0010] Further, a spray pipe with spray holes is circumferentially arranged on the inner wall of the cylindrical barrel above the adaptation port to clean the inner wall of the coating cylinder. At this time, a waste liquid recovery tank is arranged on the base directly below the conical barrel. The bracket and the telescopic rod are both located in the waste liquid recovery tank. A drain pipe extending out of the base is arranged on the bottom wall of the waste liquid recovery tank to collect the waste liquid after cleaning through the waste liquid recovery tank and quickly discharge it through the drain pipe.

[0011] The advantages of the present utility model are that through the sample loading unit, sampling unit and coating unit arranged on the base, the temporary storage of the sample liquid, the dipping of the sample liquid and the coating of the sample liquid can form a set of coherent operations, easily realizing the automatic plate coating operation. At the same time, by using the method of blowing the sampling head with the hair dryer to complete the plate coating, the sample liquid on the liquid-absorbing sponge pad dipped on the sampling head can be more evenly coated on the culture dish, realizing the uniform coating of the culture dish, reducing the labor force, solving the problem of uneven coating caused by manually shaking or rotating the culture dish, improving the standardization and unification of the plate coating operation, and improving the plate coating efficiency. Description of the Drawings

[0012] Figure 1 is a schematic structural diagram of the present utility model.

[0013] Figure 2 isFigure 1 Top view (with the liquid storage tube hidden).

[0014] Figure 3 is Figure 1 Longitudinal sectional schematic diagram of the sampling head in (exploded state).

[0015] Figure 4 is Figure 1 Longitudinal sectional schematic diagram of the coating cylinder in.

[0016] Figure 5 is Figure 4 Axonometric view (with the hair dryer hidden).

[0017] Figure 6 is Figure 1 Enlarged view of the chuck in.

[0018] Figure 7 is Figure 6 Top view. Specific implementation manner

[0019] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present utility model.

[0020] As Figure 1 、 2 shown, the experimental coating plate device described in the present utility model includes a base 1, and a sample loading unit, a sampling unit, and a coating unit that are sequentially connected are arranged on the horizontal plane at the top thereof.

[0021] Among them, the sample loading unit includes a rotating column 2 vertically arranged on the base 1. The rotating column 2 is an electric rotating column body, and a horizontal loading plate 3 is fixedly connected thereto. The horizontal loading plate 3 is a disc-shaped plate body, and its axis is consistent with the axis of the rotating column 2 and is driven by the rotating column 2 to rotate horizontally; a plurality of jacks 4 are evenly distributed along the circumference on the horizontal loading plate 3, and a liquid storage tube 5 is correspondingly arranged in each jack 4. The liquid storage tube 5 is used to hold the sample liquid to be coated, and its opening is vertically inserted upward into the jack 4 so as to dip the sample liquid from top to bottom into the liquid storage tube 5.

[0022] The sampling unit includes a lifting column 6 vertically arranged on the base. The lifting column 6 is an electric lifting column body, and its lifting end is provided with a rotating seat 7 that rotates horizontally. The rotating seat 7 can be controlled either electrically or manually. A plurality of insertion rods 8 are evenly distributed along the circumferential direction on the side wall of the rotating seat 7. The insertion rods 8 are horizontally arranged, and a sampling rod 9 is inserted therein. The outer end of the sampling rod 9 is bent vertically downward to form an inverted L-shaped structure. At the lower end of the downward-bent section of the sampling rod 9, a sampling head 10 for extending into the liquid storage tube 5 to dip the sample liquid is connected. Specifically, as Figure 3 shown, the sampling head 10 includes a horizontal ring 10.1 fixedly connected to the lower end of the sampling rod 9. The outer diameter of the horizontal ring 10.1 is smaller than the lumen diameter of the liquid storage tube 5. An annular upper gasket 10.2 and an annular lower gasket 10.3 made of rubber or silica gel are clamped inside the horizontal ring 10.1. A liquid-absorbing sponge pad 10.4 capable of adsorbing the sample liquid is clamped between the annular upper gasket 10.2 and the annular lower gasket 10.3. When one group of sampling rods 9 is driven by the rotating seat 7 to rotate to directly above one of the liquid storage tubes 5, it can be horizontally lowered by the lifting column 6 and extended into the liquid storage tube 5, so as to use the liquid-absorbing sponge pad 10.4 on the sampling head 10 to absorb the sample liquid and realize the operation of dipping the sample liquid by the sampling head.

[0023] The coating unit includes a bracket 11 vertically arranged on the base 1, and a coating cylinder 12 is mounted on the bracket 11. As Figure 4 、 5 shown, the coating cylinder 12 is composed of a cylindrical barrel 12.1 in the upper part and a conical barrel 12.2 in the lower part. An adaptation port 13 is opened in the middle of the cylindrical barrel 12.1. The adaptation port 13 matches the sampling head 10 (that is, the adaptation port 13 is the same size as the horizontal ring 10.1 that constitutes the sampling head 10), so that the sampling head 10 can be horizontally inserted into the adaptation port 13 and form a sealed fit between the horizontal ring 10.1 and the outer wall of the cylindrical barrel 12.1. A hair dryer 14 with a downward-facing air outlet is also arranged at the top of the cylindrical barrel 12.1. The hair dryer 14 can blow vertically downward on the sampling head 10 inserted into the adaptation port 13. In addition, a telescopic rod 15 is vertically arranged on the base 1 directly below the conical barrel 12.2. A chuck 16 for horizontally clamping the culture dish to be coated is arranged at the upper end of the telescopic rod 15. Specifically, as Figure 6 、 7 shown, the chuck 16 includes a clamping seat 16.1 fixedly connected to the upper end of the telescopic rod 15. The top surface of the clamping seat 16.1 is horizontal and is used to stably support the culture dish to be coated. A pair of horizontally sliding clamping arms 16.3 are connected to the top surface of the clamping seat 16.1 through springs 16.2. Arc-shaped clamping plates 16.4 for clamping the outer edge of the culture dish to be coated are arranged at the outer ends of the two horizontally clamping arms 16.3.

[0024] In use, first, the liquid storage tubes 5 containing the sample liquid to be coated are inserted one by one into each jack 4 on the horizontal carrier plate 3. Then, the rotating column 2 is controlled to rotate, so that the horizontal carrier plate 3 drives the liquid storage tubes 5 to rotate. When one of the liquid storage tubes 5 rotates to the closest position to the lifting column 6 of the sampling unit, the rotating column 2 stops. At this time, this liquid storage tube 5 reaches the working position.

[0025] Then, the rotating seat 7 drives the insertion rod 8 to rotate manually or electrically, so that the sampling heads 10 on the sampling rod 9 rotate one by one to directly above the liquid storage tube 5 that reaches the working position. And when one of the sampling heads 10 rotates to directly above the liquid storage tube 5 that reaches the working position, the remaining sampling heads 10 will just be staggered with the coating unit. At this time, when the lifting column 6 descends, the sampling head 10 located directly above the liquid storage tube 5 can be translated downward and inserted into the liquid storage tube 5 to dip the sample liquid. After the dipping is completed, the lifting column 6 rises again to move the sampling head 10 out of the liquid storage tube 5.

[0026] After that, the rotating seat 7 is rotated again to horizontally insert the sampling head 10 that has completed dipping into the fitting port 13. When the sampling head 10 that has completed dipping is horizontally inserted into the fitting port 13, the telescopic rod 15 below the coating cylinder 12 rises, pushing the chuck 16 that clamps the culture dish to be coated upward, so that the upper mouth edge of the culture dish to be coated abuts against the inner wall of the conical cylinder 12.2, forming a sealed chamber above the culture dish to be coated. At this time, the hair dryer 14 is started to blow the sampling head 10 inserted into the fitting port 13, so as to evenly blow the sample liquid adsorbed by the liquid absorption sponge pad 10.4 on the sampling head 10 onto the culture dish to be coated, completing the plate coating operation of the culture dish, realizing the uniform coating of the culture dish, reducing the labor force, solving the problem of uneven coating caused by manually shaking or rotating the culture dish, improving the standardization and uniformity of the plate coating operation, and improving the plate coating efficiency.

[0027] In addition, in order to be able to clean the inner wall of the coating cylinder 12 after the plate coating is completed, a spray pipe 17 with spray holes can also be circumferentially arranged along the inner wall of the cylindrical cylinder 12.1 above the fitting port 13. The liquid inlet end of the spray pipe 17 is hermetically passed through the outside of the coating cylinder 12, and the liquid inlet end of the spray pipe 17 is connected to an external cleaning pump, so that the inner wall of the coating cylinder 12 can be quickly rinsed through the spray pipe 17. In addition, in order to prevent the waste liquid after cleaning from flowing out and causing pollution, a waste liquid recovery tank 18 can also be provided on the base 1 directly opposite to the lower part of the conical cylinder 12.2, and a drain pipe 19 extending through the outside of the base 1 is provided on the bottom wall of the waste liquid recovery tank 18. The waste liquid after cleaning is collected through the waste liquid recovery tank 18 and quickly discharged through the drain pipe 19. At this time, the bracket 11 and the telescopic rod 15 should both be located in the waste liquid recovery tank 18, and an annular rubber sealing gasket is provided at the telescopic socket of the telescopic rod 15, which can prevent the liquid from entering the telescopic rod 15 and ensure its normal operation.

[0028] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present utility model are only used to explain the relative positional relationship, movement conditions, etc. between components in a certain specific posture (as shown in the attached drawings). If the specific posture changes, the directional indications will also change accordingly.

[0029] In addition, in the present utility model, unless otherwise clearly specified and limited, terms such as "connection" and "fixation" should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal connection of two components or the interaction relationship between two components, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

Claims

1. An experimental coating plate device, characterized in that: It includes a base, on which a sample loading unit, a sampling unit and a coating unit are provided; Among them, the sample loading unit includes a rotating column vertically arranged on the base. A horizontal carrier plate is fixedly connected to the rotating column. A plurality of jacks are evenly distributed along the circumference on the horizontal carrier plate. A liquid storage tube for containing sample liquid is arranged in the jack; The sampling unit includes a lifting column vertically arranged on the base. A rotating seat that rotates horizontally is arranged at the lifting end of the lifting column. A plurality of insertion rods are evenly distributed along the circumference on the side wall of the rotating seat. A sampling rod is inserted into the insertion rod. The outer end of the sampling rod bends vertically downward, and a sampling head for extending into the liquid storage tube to dip the sample liquid is connected to its lower end; The coating unit includes a bracket vertically arranged on the base. A coating cylinder is mounted on the bracket. The coating cylinder is composed of a cylindrical barrel in the upper part and a conical barrel in the lower part. An adaptation port matching the sampling head is opened in the middle of the cylindrical barrel. A hair dryer with a downward air outlet is arranged at the top of the cylindrical barrel. A telescopic rod is vertically arranged on the base directly below the conical barrel. A chuck for horizontally clamping the culture dish to be coated is arranged at the upper end of the telescopic rod.

2. The experimental coating plate device according to claim 1, characterized in that: The sampling head includes a horizontal ring fixedly connected to the lower end of the sampling rod. An annular upper gasket and an annular lower gasket are clamped in the horizontal ring. A liquid absorption sponge pad is clamped between the annular upper gasket and the annular lower gasket.

3. The experimental coating plate device according to claim 1, characterized in that: The chuck includes a clamping seat fixedly connected to the upper end of the telescopic rod. A pair of horizontally sliding clamping arms are connected to the top surface of the clamping seat through springs. Arc-shaped clamping plates for clamping the outer edge of the culture dish to be coated are arranged at the outer ends of the two horizontal clamping arms.

4. The experimental coating plate device according to claim 1, characterized in that: A spray pipe with spray holes is arranged along the circumference on the inner wall of the cylindrical barrel above the adaptation port.

5. The experimental coating plate device according to claim 1, characterized in that: A waste liquid recovery tank is arranged on the base directly below the conical barrel. The bracket and the telescopic rod are both located in the waste liquid recovery tank. A drain pipe extending out of the base is arranged on the bottom wall of the waste liquid recovery tank.

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